AT4G05180

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012961
Physical Location & Seq
Reverse (-)
2671523 .. 2673243
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05180.1

Sequence Viewer

Length: 693 bp
ATGGCTCAAGCAGTGACTTCGATGGCTGGCTTACGTGGAGCATCTCAGGCTGTCCTTGAAGGAAGTTTACAGATCAACGGCTCAAACCGTTTGAACATCTCAAGAGTCTCGGTTGGGTCTCAGAGAACCGGACTTGTGATCAGGGCTCAGCAGAACGTGTCAGTACCAGAAAGTAGTCGCCGGTCAGTGATTGGACTCGTGGCGGCTGGTTTAGCCGGTGGTTCATTCGTTAAAGCTGTTTTCGCCGAAGCTATTCCGATCAAAGTTGGTGGTCCTCCACTTCCTTCCGGTGGCCTACCTGGAACAGATAACTCAGACCAAGCAAGAGACTTTTCATTGGCATTGAAAGATAGATTTTACATACAACCATTGTCACCAACAGAAGCTGCAGCTAGAGCCAAAGATTCTGCTAAAGAGATCATCAACGTTAAGTCATTTATCGACAAAAAAGCTTGGCCCTATGTTCAGAACGATCTCCGTTTAAGAGCATCGTACCTCCGTTACGATCTCAACACCGTTATCTCCGCTAAGCCTAAGGAAGAGAAGCAAAGCCTTAAAGATCTCACCGCAAAGCTTTTCCAAACCATTGACAACTTGGACTATGCGGCGAGATCAAAGAGTAGCCCAGATGCTGAGAAGTATTACTCAGAAACTGTCTCGAGTTTGAACAATGTTCTTGCCAAGCTCGGTTAA

Protein Analysis

230

Amino Acids

24.64

Weight (kDa)

9.72

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbQ PF05757 1 - 230 9.1e-78 Oxygen evolving enhancer protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012961)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05180 AT4G05180
fragaria_vesca FvH4_3g02920
malus_domestica MD05G1336200.v1.1 MD10G1311700.v1.1
prunus_persica Prupe.4G029300_v2.0.a1
pyrus_communis pycom05g30770 pycom10g26290
rosa_chinensis RchiOBHm_Chr5g0004501
rosa_laevigata RLG00000031234
rosa_multiflora Rmu_sc0000485.1_g000003
rosa_roxburghii Rroxscaffold_1G00071020
rosa_rugosa Rorug04G0412200
rosa_samantha Rh5AG039900 Rh5BG038900 Rh5CG043000 Rh5DG038800
rosa_wichuraiana Rw5G003810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 203, 525, 567, 605
AclI AACGTT 1 cut(s) 426
AfaI GTAC 2 cut(s) 165, 494
AfiI CCNNNNNNNGG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 4 cut(s) 59, 94, 346, 667
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 7 cut(s) 236, 251, 386, 392, 452, 574, 685
AluI AGCT 7 cut(s) 236, 251, 386, 392, 452, 574, 685
Alw26I GTCTC 4 cut(s) 112, 123, 321, 661
AlwNI CAGNNNCTG 3 cut(s) 386, 632, 653
Ama87I CYCGRG 1 cut(s) 658
AoxI GGCC 2 cut(s) 292, 455
ApeKI GCWGC 2 cut(s) 386, 389
Asp700I GAANNNNTTC 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 272, 456
AsuHPI GGTGA 2 cut(s) 366, 556
AvaI CYCGRG 1 cut(s) 658
AvaII GGWCC 1 cut(s) 272
AxyI CCTNAGG 1 cut(s) 534
BanII GRGCYC 1 cut(s) 148
BarI GAAGNNNNNNTAC 2 cut(s) 51, 83
BauI CACGAG 1 cut(s) 197
BbvI GCAGC 2 cut(s) 373, 401
BccI CCATC 1 cut(s) 16
BceAI ACGGC 1 cut(s) 94
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 300
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 4 cut(s) 112, 123, 321, 661
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 387
BglII AGATCT 1 cut(s) 559
BisI GCNGC 4 cut(s) 204, 387, 390, 606
BlpI GCTNAGC 2 cut(s) 147, 528
BlsI GCNGC 4 cut(s) 205, 388, 391, 607
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 658
BmgT120I GGNCC 2 cut(s) 272, 456
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 3 cut(s) 50, 497, 619
Bpu1102I GCTNAGC 2 cut(s) 147, 528
BpuEI CTTGAG 1 cut(s) 85
BsaAI YACGTR 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 123
BsaWI WCCGGW 2 cut(s) 128, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse118I RCCGGY 2 cut(s) 180, 215
Bse21I CCTNAGG 1 cut(s) 534
BseBI CCWGG 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 290
BseMII CTCAG 6 cut(s) 59, 134, 161, 327, 624, 660
BseXI GCAGC 2 cut(s) 373, 401
BshFI GGCC 2 cut(s) 294, 457
BsiHKCI CYCGRG 1 cut(s) 658
BsiSI CCGG 4 cut(s) 129, 181, 216, 288
BslI CCNNNNNNNGG 1 cut(s) 290
BsmAI GTCTC 4 cut(s) 112, 123, 321, 661
BsnI GGCC 2 cut(s) 294, 457
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 658
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
Bsp1720I GCTNAGC 2 cut(s) 147, 528
BspACI CCGC 4 cut(s) 203, 525, 567, 605
BspANI GGCC 2 cut(s) 294, 457
BspCNI CTCAG 6 cut(s) 58, 133, 160, 326, 625, 659
BspMAI CTGCAG 1 cut(s) 391
BspTNI GGTCTC 1 cut(s) 123
BsrFI RCCGGY 2 cut(s) 180, 215
BssAI RCCGGY 2 cut(s) 180, 215
BssMI GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
BssSI CACGAG 1 cut(s) 197
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 3 cut(s) 89, 517, 655
Bst6I CTCTTC 1 cut(s) 534
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 8 cut(s) 45, 120, 147, 313, 528, 534, 633, 646
BstKTI GATC 8 cut(s) 75, 141, 261, 420, 475, 508, 562, 614
BstMAI GTCTC 4 cut(s) 112, 123, 321, 661
BstMBI GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
BstMWI GCNNNNNNNGC 4 cut(s) 47, 212, 242, 395
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 2 cut(s) 373, 401
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 2 cut(s) 294, 457
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 18, 192
Cac8I GCNNGC 1 cut(s) 28
CaiI CAGNNNCTG 3 cut(s) 386, 632, 653
Cfr10I RCCGGY 2 cut(s) 180, 215
Cfr13I GGNCC 2 cut(s) 272, 456
Csp6I GTAC 2 cut(s) 164, 493
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviQI GTAC 2 cut(s) 164, 493
DdeI CTNAG 8 cut(s) 45, 120, 147, 313, 528, 534, 633, 646
DpnI GATC 8 cut(s) 74, 140, 260, 419, 474, 507, 561, 613
DpnII GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 148
FaiI YATR 3 cut(s) 362, 462, 603
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 204, 387, 390, 606
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 4 cut(s) 204, 387, 390, 606
FspBI CTAG 1 cut(s) 393
GluI GCNGC 4 cut(s) 204, 387, 390, 606
HaeIII GGCC 2 cut(s) 294, 457
HapII CCGG 4 cut(s) 129, 181, 216, 288
HindIII AAGCTT 2 cut(s) 450, 572
HinfI GANTC 3 cut(s) 105, 195, 404
HpaII CCGG 4 cut(s) 129, 181, 216, 288
HphI GGTGA 2 cut(s) 366, 556
Hpy166II GTNNAC 1 cut(s) 68
Hpy188I TCNGA 5 cut(s) 123, 258, 316, 468, 649
Hpy188III TCNNGA 2 cut(s) 102, 658
Hpy8I GTNNAC 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 53, 294
HpyCH4III ACNGT 3 cut(s) 89, 517, 655
HpyCH4IV ACGT 3 cut(s) 34, 156, 426
HpyCH4V TGCA 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 212, 242, 395
HpyF3I CTNAG 8 cut(s) 45, 120, 147, 313, 528, 534, 633, 646
HpySE526I ACGT 3 cut(s) 34, 156, 426
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 2 cut(s) 373, 401
LweI GCATC 3 cut(s) 50, 497, 619
MaeI CTAG 1 cut(s) 393
MaeII ACGT 3 cut(s) 34, 156, 426
MaeIII GTNAC 3 cut(s) 13, 372, 500
MalI GATC 8 cut(s) 74, 140, 260, 419, 474, 507, 561, 613
MboI GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
MboII GAAGA 1 cut(s) 551
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 148
MlyI GAGTC 2 cut(s) 114, 189
MnlI CCTC 2 cut(s) 285, 506
MroXI GAANNNNTTC 1 cut(s) 252
MseI TTAA 5 cut(s) 231, 429, 482, 555, 691
MspI CCGG 4 cut(s) 129, 181, 216, 288
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 4 cut(s) 47, 212, 242, 395
NdeII GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
NmuCI GTSAC 2 cut(s) 13, 372
PaeR7I CTCGAG 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 4 cut(s) 205, 388, 391, 607
PleI GAGTC 2 cut(s) 113, 189
PpsI GAGTC 2 cut(s) 113, 189
Ppu21I YACGTR 1 cut(s) 35
Psp1406I AACGTT 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspPI GGNCC 2 cut(s) 272, 456
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 3 cut(s) 386, 632, 653
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 2 cut(s) 165, 494
RsaNI GTAC 2 cut(s) 164, 493
SaqAI TTAA 5 cut(s) 231, 429, 482, 555, 691
SatI GCNGC 4 cut(s) 204, 387, 390, 606
Sau3AI GATC 8 cut(s) 72, 138, 258, 417, 472, 505, 559, 611
Sau96I GGNCC 2 cut(s) 272, 456
SchI GAGTC 2 cut(s) 114, 189
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 3 cut(s) 50, 497, 619
SfcI CTRYAG 1 cut(s) 387
Sfr274I CTCGAG 1 cut(s) 658
SinI GGWCC 1 cut(s) 272
SlaI CTCGAG 1 cut(s) 658
SmlI CTYRAG 3 cut(s) 6, 100, 658
SmoI CTYRAG 3 cut(s) 6, 100, 658
SsiI CCGC 4 cut(s) 203, 525, 567, 605
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 298
TaaI ACNGT 3 cut(s) 89, 517, 655
TaiI ACGT 3 cut(s) 37, 159, 429
TaqI TCGA 3 cut(s) 20, 441, 659
TauI GCSGC 2 cut(s) 206, 608
TfiI GAWTC 1 cut(s) 404
Tru1I TTAA 5 cut(s) 231, 429, 482, 555, 691
Tru9I TTAA 5 cut(s) 231, 429, 482, 555, 691
TscAI CASTG 2 cut(s) 18, 192
TseFI GTSAC 2 cut(s) 13, 372
TseI GCWGC 2 cut(s) 386, 389
Tsp45I GTSAC 2 cut(s) 13, 372
TspDTI ATGAA 2 cut(s) 213, 324
TspGWI ACGGA 2 cut(s) 467, 488
TspRI CASTG 2 cut(s) 18, 192
VpaK11BI GGWCC 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.