AT4G05220

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015045
Physical Location & Seq
Reverse (-)
2684997 .. 2685793
797 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05220.1

Sequence Viewer

Length: 681 bp
ATGACACCAGACCGAACAACTATCCCGATCCGCACCAGTCCCGTACCCCGAGCCCAACCCATGAAGCGTCACCATTCCGCTAGCTACTACGCTCACCGTGTCAGAGAAAGCCTCTCAACCCGGATATCGAAATTCATCTGCGCAATGTTTCTCTTGGTTTTGTTCTTTGTAGGCGTCATTGCTTTCATACTTTGGCTCAGTTTACGCCCTCATAGACCTCGGTTCCATATCCAAGACTTCGTGGTACAAGGTCTAGACCAGCCTACCGGAGTCGAAAACGCAAGAATTGCTTTCAATGTAACCATACTTAACCCTAACCAACATATGGGTGTTTACTTTGATTCCATGGAAGGTTCCATTTACTACAAAGATCAACGGGTCGGATTGATCCCATTGCTAAACCCGTTTTTCCAGCAACCGACTAACACAACAATTGTGACCGGGACGCTTACAGGAGCTTCCTTAACGGTAAACAGTAACCGCTGGACCGAGTTTAGTAATGATCGGGCTCAAGGAACCGTGGGTTTCAGGCTCGATATAGTTTCGACTATTCGGTTTAAACTCCATAGGTGGATCAGTAAGCATCATAGGATGCACGCTAATTGCAACATCGTGGTTGGAAGAGACGGGTTGATCTTGCCCAAGTTTAACCATAAACGGTGTCCAGTCTACTTCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

226

Amino Acids

26.03

Weight (kDa)

10.59

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 100 - 202 2.3e-13 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05220
fragaria_vesca FvH4_3g02710
malus_domestica MD05G1340700.v1.1 MD10G1314200.v1.1
prunus_persica Prupe.4G026800_v2.0.a1
pyrus_communis pycom05g30990 pycom10g26620
rosa_chinensis RchiOBHm_Chr5g0004071
rosa_laevigata RLG00000031201
rosa_multiflora Rmu_sc0000547.1_g000030
rosa_rugosa Rorug04G0409800
rosa_samantha Rh5AG037300 Rh5BG036300 Rh5CG040000 Rh5DG035800
rosa_wichuraiana Rw5G003370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 142
AccB7I CCANNNNNTGG 1 cut(s) 325
AccI GTMKAC 1 cut(s) 669
AciI CCGC 3 cut(s) 31, 78, 481
AclWI GGATC 3 cut(s) 22, 382, 581
AcsI RAATTY 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 2 cut(s) 45, 246
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 1 cut(s) 295
AluBI AGCT 2 cut(s) 84, 458
AluI AGCT 2 cut(s) 84, 458
Alw26I GTCTC 1 cut(s) 618
AlwI GGATC 3 cut(s) 22, 382, 581
Ama87I CYCGRG 1 cut(s) 48
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 486
AsuC2I CCSGG 2 cut(s) 121, 442
AsuHPI GGTGA 2 cut(s) 62, 86
AsuNHI GCTAGC 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 48
AvaII GGWCC 1 cut(s) 486
BanII GRGCYC 2 cut(s) 55, 511
BcnI CCSGG 2 cut(s) 121, 442
BcoDI GTCTC 1 cut(s) 618
BfaI CTAG 2 cut(s) 81, 254
Bme1390I CCNGG 2 cut(s) 121, 442
Bme18I GGWCC 1 cut(s) 486
BmeT110I CYCGRG 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 486
BmiI GGNNCC 3 cut(s) 224, 355, 517
BmrFI CCNGG 2 cut(s) 121, 442
BmsI GCATC 2 cut(s) 582, 592
BmtI GCTAGC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BpuEI CTTGAG 1 cut(s) 495
BpuMI CCSGG 2 cut(s) 121, 442
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 3 cut(s) 218, 345, 519
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 36, 665
Bse3DI GCAATG 3 cut(s) 150, 177, 392
BseDI CCNNGG 3 cut(s) 218, 345, 519
BseGI GGATG 1 cut(s) 597
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 3 cut(s) 150, 177, 392
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 2 cut(s) 36, 665
BsiHKCI CYCGRG 1 cut(s) 48
BsiSI CCGG 3 cut(s) 121, 267, 441
BslFI GGGAC 2 cut(s) 24, 457
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 1 cut(s) 618
BsmBI CGTCTC 1 cut(s) 618
BsmFI GGGAC 2 cut(s) 24, 457
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 2 cut(s) 55, 511
Bsp143I GATC 6 cut(s) 27, 370, 387, 502, 573, 633
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 3 cut(s) 31, 78, 481
BspCNI CTCAG 1 cut(s) 210
BspLI GGNNCC 3 cut(s) 224, 355, 517
BspOI GCTAGC 1 cut(s) 84
BspPI GGATC 3 cut(s) 22, 382, 581
BsrDI GCAATG 3 cut(s) 150, 177, 392
BsrI ACTGG 2 cut(s) 36, 665
BssECI CCNNGG 3 cut(s) 218, 345, 519
BssMI GATC 6 cut(s) 27, 370, 387, 502, 573, 633
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 345
Bst4CI ACNGT 5 cut(s) 98, 469, 476, 520, 660
Bst6I CTCTTC 1 cut(s) 616
BstACI GRCGYC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 82, 597
BstDEI CTNAG 2 cut(s) 197, 678
BstDSI CCRYGG 2 cut(s) 345, 519
BstF5I GGATG 1 cut(s) 597
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 6 cut(s) 30, 373, 390, 505, 576, 636
BstMAI GTCTC 1 cut(s) 618
BstMBI GATC 6 cut(s) 27, 370, 387, 502, 573, 633
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 119, 440
BtgI CCRYGG 2 cut(s) 345, 519
BtsCI GGATG 1 cut(s) 597
Cac8I GCNNGC 2 cut(s) 82, 597
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 486
CseI GACGC 3 cut(s) 56, 163, 454
Csp6I GTAC 2 cut(s) 44, 245
CviAII CATG 2 cut(s) 61, 346
CviJI RGCY 8 cut(s) 53, 84, 111, 196, 262, 458, 509, 532
CviKI_1 RGCY 8 cut(s) 53, 84, 111, 196, 262, 458, 509, 532
CviQI GTAC 2 cut(s) 44, 245
DdeI CTNAG 2 cut(s) 197, 678
DpnI GATC 6 cut(s) 29, 372, 389, 504, 575, 635
DpnII GATC 6 cut(s) 27, 370, 387, 502, 573, 633
DraI TTTAAA 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 616
EarI CTCTTC 1 cut(s) 616
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 2 cut(s) 55, 511
Eco32I GATATC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 48
EcoRV GATATC 1 cut(s) 126
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 2 cut(s) 55, 511
ErhI CCWWGG 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 2 cut(s) 64, 349
FalI AAGNNNNNCTT 2 cut(s) 274, 306
FaqI GGGAC 2 cut(s) 24, 457
FatI CATG 2 cut(s) 60, 345
FauNDI CATATG 1 cut(s) 324
FblI GTMKAC 1 cut(s) 669
FokI GGATG 1 cut(s) 604
FriOI GRGCYC 2 cut(s) 55, 511
FspBI CTAG 2 cut(s) 81, 254
FspI TGCGCA 1 cut(s) 142
GlaI GCGC 1 cut(s) 142
HapII CCGG 3 cut(s) 121, 267, 441
HgaI GACGC 3 cut(s) 56, 163, 454
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 2 cut(s) 64, 349
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HinfI GANTC 2 cut(s) 270, 341
HpaII CCGG 3 cut(s) 121, 267, 441
HphI GGTGA 2 cut(s) 62, 86
Hpy166II GTNNAC 4 cut(s) 203, 334, 472, 670
Hpy188I TCNGA 2 cut(s) 104, 383
Hpy188III TCNNGA 2 cut(s) 25, 254
Hpy8I GTNNAC 4 cut(s) 203, 334, 472, 670
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 5 cut(s) 98, 469, 476, 520, 660
HpyCH4V TGCA 2 cut(s) 595, 606
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 2 cut(s) 197, 678
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 2 cut(s) 64, 349
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 6 cut(s) 27, 370, 387, 502, 573, 633
LmnI GCTCC 1 cut(s) 455
LweI GCATC 2 cut(s) 582, 592
MaeI CTAG 2 cut(s) 81, 254
MaeIII GTNAC 4 cut(s) 68, 298, 436, 476
MalI GATC 6 cut(s) 29, 372, 389, 504, 575, 635
MboI GATC 6 cut(s) 27, 370, 387, 502, 573, 633
MboII GAAGA 1 cut(s) 633
MfeI CAATTG 1 cut(s) 432
MhlI GDGCHC 2 cut(s) 55, 511
MluCI AATT 4 cut(s) 131, 285, 432, 601
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 361, 598
MnlI CCTC 3 cut(s) 122, 219, 228
MseI TTAA 4 cut(s) 309, 464, 558, 648
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 483
MspI CCGG 3 cut(s) 121, 267, 441
MspR9I CCNGG 2 cut(s) 121, 442
MssI GTTTAAAC 1 cut(s) 559
MunI CAATTG 1 cut(s) 432
MwoI GCNNNNNNNGC 1 cut(s) 287
NciI CCSGG 2 cut(s) 121, 442
NcoI CCATGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 324
NdeII GATC 6 cut(s) 27, 370, 387, 502, 573, 633
NheI GCTAGC 1 cut(s) 80
NlaIII CATG 2 cut(s) 64, 349
NlaIV GGNNCC 3 cut(s) 224, 355, 517
NmuCI GTSAC 2 cut(s) 68, 436
NsbI TGCGCA 1 cut(s) 142
PfeI GAWTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 325
PleI GAGTC 1 cut(s) 278
PmeI GTTTAAAC 1 cut(s) 559
PpsI GAGTC 1 cut(s) 278
PspN4I GGNNCC 3 cut(s) 224, 355, 517
PspPI GGNCC 1 cut(s) 486
RsaI GTAC 2 cut(s) 45, 246
RsaNI GTAC 2 cut(s) 44, 245
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 4 cut(s) 309, 464, 558, 648
Sau3AI GATC 6 cut(s) 27, 370, 387, 502, 573, 633
Sau96I GGNCC 1 cut(s) 486
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 2 cut(s) 121, 442
SduI GDGCHC 2 cut(s) 55, 511
SetI ASST 6 cut(s) 86, 220, 253, 355, 460, 572
SfaNI GCATC 2 cut(s) 582, 592
SinI GGWCC 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
Sse9I AATT 4 cut(s) 131, 285, 432, 601
SsiI CCGC 3 cut(s) 31, 78, 481
SspMI CTAG 2 cut(s) 81, 254
StyD4I CCNGG 2 cut(s) 119, 440
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 5 cut(s) 98, 469, 476, 520, 660
TaqI TCGA 4 cut(s) 128, 273, 534, 545
TaqII GACCGA 2 cut(s) 27, 503
TasI AATT 4 cut(s) 131, 285, 432, 601
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 4 cut(s) 309, 464, 558, 648
Tru9I TTAA 4 cut(s) 309, 464, 558, 648
TseFI GTSAC 2 cut(s) 68, 436
Tsp45I GTSAC 2 cut(s) 68, 436
TspDTI ATGAA 3 cut(s) 77, 124, 175
Van91I CCANNNNNTGG 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 486
XapI RAATTY 1 cut(s) 131
XbaI TCTAGA 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 669
XspI CTAG 2 cut(s) 81, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.