MD10G1314200.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015045
Physical Location & Seq
Reverse (-)
39837962 .. 39838642
681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1314200.v1.1.491

Sequence Viewer

Length: 681 bp
ATGTACAACTCCGACCAGTTGCCGATAGAGTACGCCACCCCAGAGCAACAACGACCCATGAAGCGTCAACACACCGCCCGGTACTACGCCCACCGCGTCCGCGAAAGTCTCACCACCCGGGTCTCCAAGGTCATATGCACCATTTTCTTAGGCCTCCTCCTCATCGTTGGCATCATAGCGTTCATACTATGGCTCAGTCTACGTCCGCACCGCCCAAGATTTCATATCCTTGCATTTTCGGTTCCGGGTTTGGCTCAAGAATCCGGGTTCGAAAATGCTGAGATAACGTTCAATGCCACGGCCCGTAACGCGAACCATAATATTGGCATATATTATGGTTCTATGGACGGGACGGTGTACTATAGGGACCAACGGATCGGGTCAATAACGGGTTTACTTCAACCGTTTTATCAGGATCCGAAGAATACGACGATCGTGATGGGGTCGTTTAGGGGGGCCACCTTAAATGTTAATAACCAGCGTTGGACGGAGTTCTTGAATGATCGTAAAAGAGGAACGGTTGTGTTTCGGCTTGAATTTACGGCCGCGATCAGATTTAAGATTCGCGCTTGGTACAGCAAGCGCCATCGGATGCACGCCACCTGTGATGTTGATGTGGGCCCGGACGGCTTGATTTTGCCCATTTCTAAAGATAGGAGATGCCCTGTGTATTTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

227

Amino Acids

26.15

Weight (kDa)

10.0

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 100 - 202 2.5e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05220
fragaria_vesca FvH4_3g02710
malus_domestica MD05G1340700.v1.1 MD10G1314200.v1.1
prunus_persica Prupe.4G026800_v2.0.a1
pyrus_communis pycom05g30990 pycom10g26620
rosa_chinensis RchiOBHm_Chr5g0004071
rosa_laevigata RLG00000031201
rosa_multiflora Rmu_sc0000547.1_g000030
rosa_rugosa Rorug04G0409800
rosa_samantha Rh5AG037300 Rh5BG036300 Rh5CG040000 Rh5DG035800
rosa_wichuraiana Rw5G003370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 199
AccII CGCG 5 cut(s) 96, 102, 311, 548, 567
AciI CCGC 6 cut(s) 75, 94, 100, 206, 211, 546
AclI AACGTT 1 cut(s) 287
AclWI GGATC 3 cut(s) 383, 410, 423
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 1 cut(s) 536
AfaI GTAC 5 cut(s) 5, 32, 83, 359, 575
AgsI TTSAA 4 cut(s) 292, 401, 499, 536
Alw26I GTCTC 2 cut(s) 113, 127
AlwI GGATC 3 cut(s) 383, 410, 423
Ama87I CYCGRG 1 cut(s) 117
AoxI GGCC 5 cut(s) 151, 300, 456, 543, 619
ApaI GGGCCC 1 cut(s) 623
ApoI RAATTY 1 cut(s) 536
AspLEI GCGC 2 cut(s) 569, 585
AspS9I GGNCC 5 cut(s) 301, 367, 456, 619, 620
AsuC2I CCSGG 6 cut(s) 79, 118, 119, 246, 265, 623
AsuHPI GGTGA 2 cut(s) 103, 667
AsuII TTCGAA 1 cut(s) 270
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 623
BamHI GGATCC 1 cut(s) 415
BanII GRGCYC 1 cut(s) 623
BccI CCATC 2 cut(s) 433, 594
BceAI ACGGC 3 cut(s) 315, 558, 643
BcnI CCSGG 6 cut(s) 79, 118, 119, 246, 265, 623
BcoDI GTCTC 2 cut(s) 113, 127
BfmI CTRYAG 1 cut(s) 361
BfoI RGCGCY 1 cut(s) 586
BglI GCCNNNNNGGC 1 cut(s) 627
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 6 cut(s) 79, 118, 119, 246, 265, 623
Bme18I GGWCC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 5 cut(s) 301, 367, 456, 619, 620
BmiI GGNNCC 5 cut(s) 243, 368, 417, 457, 621
BmrFI CCNGG 6 cut(s) 79, 118, 119, 246, 265, 623
BmsI GCATC 3 cut(s) 180, 582, 650
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 240
BpuMI CCSGG 6 cut(s) 79, 118, 119, 246, 265, 623
BsaI GGTCTC 1 cut(s) 127
BsaJI CCNNGG 3 cut(s) 117, 126, 297
Bse1I ACTGG 1 cut(s) 16
BseDI CCNNGG 3 cut(s) 117, 126, 297
BseGI GGATG 1 cut(s) 597
BseMII CTCAG 2 cut(s) 208, 270
BseNI ACTGG 1 cut(s) 16
BseRI GAGGAG 2 cut(s) 146, 149
BseSI GKGCMC 1 cut(s) 623
BseX3I CGGCCG 1 cut(s) 543
Bsh1236I CGCG 5 cut(s) 96, 102, 311, 548, 567
Bsh1285I CGRYCG 2 cut(s) 435, 546
BshFI GGCC 5 cut(s) 153, 302, 458, 545, 621
BsiEI CGRYCG 2 cut(s) 435, 546
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 5 cut(s) 79, 118, 245, 264, 623
BslFI GGGAC 2 cut(s) 364, 380
BsmAI GTCTC 2 cut(s) 113, 127
BsmFI GGGAC 2 cut(s) 364, 380
BsnI GGCC 5 cut(s) 153, 302, 458, 545, 621
Bso31I GGTCTC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 270
Bsp120I GGGCCC 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 623
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 375, 415, 432, 502, 549
BspACI CCGC 6 cut(s) 75, 94, 100, 206, 211, 546
BspANI GGCC 5 cut(s) 153, 302, 458, 545, 621
BspCNI CTCAG 2 cut(s) 207, 271
BspFNI CGCG 5 cut(s) 96, 102, 311, 548, 567
BspLI GGNNCC 5 cut(s) 243, 368, 417, 457, 621
BspPI GGATC 3 cut(s) 383, 410, 423
BspT104I TTCGAA 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 127
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 117, 126, 297
BssMI GATC 5 cut(s) 375, 415, 432, 502, 549
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 3 cut(s) 355, 405, 520
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 581, 597
BstDEI CTNAG 3 cut(s) 148, 194, 279
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 1 cut(s) 597
BstFNI CGCG 5 cut(s) 96, 102, 311, 548, 567
BstH2I RGCGCY 1 cut(s) 586
BstHHI GCGC 2 cut(s) 569, 585
BstKTI GATC 5 cut(s) 378, 418, 435, 505, 552
BstMAI GTCTC 2 cut(s) 113, 127
BstMBI GATC 5 cut(s) 375, 415, 432, 502, 549
BstMCI CGRYCG 2 cut(s) 435, 546
BstMWI GCNNNNNNNGC 2 cut(s) 308, 627
BstSCI CCNGG 6 cut(s) 77, 116, 117, 244, 263, 621
BstSFI CTRYAG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 623
BstUI CGCG 5 cut(s) 96, 102, 311, 548, 567
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 323
BstYI RGATCY 1 cut(s) 415
BstZI CGGCCG 1 cut(s) 543
BsuRI GGCC 5 cut(s) 153, 302, 458, 545, 621
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 597
Cac8I GCNNGC 2 cut(s) 581, 597
CfoI GCGC 2 cut(s) 569, 585
Cfr13I GGNCC 5 cut(s) 301, 367, 456, 619, 620
Cfr9I CCCGGG 1 cut(s) 117
CseI GACGC 2 cut(s) 53, 85
Csp6I GTAC 5 cut(s) 4, 31, 82, 358, 574
CviAII CATG 1 cut(s) 58
CviJI RGCY 9 cut(s) 153, 193, 254, 302, 458, 532, 545, 621, 630
CviKI_1 RGCY 9 cut(s) 153, 193, 254, 302, 458, 532, 545, 621, 630
CviQI GTAC 5 cut(s) 4, 31, 82, 358, 574
DdeI CTNAG 3 cut(s) 148, 194, 279
DpnI GATC 5 cut(s) 377, 417, 434, 504, 551
DpnII GATC 5 cut(s) 375, 415, 432, 502, 549
EaeI YGGCCR 1 cut(s) 543
EagI CGGCCG 1 cut(s) 543
EclXI CGGCCG 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 126
Eco147I AGGCCT 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 623
Eco31I GGTCTC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 367
Eco52I CGGCCG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 623
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 1 cut(s) 61
FaqI GGGAC 2 cut(s) 364, 380
FatI CATG 1 cut(s) 57
FauNDI CATATG 1 cut(s) 134
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 1 cut(s) 604
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 1 cut(s) 546
GlaI GCGC 2 cut(s) 568, 584
GluI GCNGC 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 586
HaeIII GGCC 5 cut(s) 153, 302, 458, 545, 621
HapII CCGG 5 cut(s) 79, 118, 245, 264, 623
HgaI GACGC 2 cut(s) 53, 85
HhaI GCGC 2 cut(s) 569, 585
Hin1II CATG 1 cut(s) 61
Hin6I GCGC 2 cut(s) 567, 583
HinP1I GCGC 2 cut(s) 567, 583
HincII GTYRAC 1 cut(s) 68
HindII GTYRAC 1 cut(s) 68
HinfI GANTC 2 cut(s) 260, 562
HpaII CCGG 5 cut(s) 79, 118, 245, 264, 623
HphI GGTGA 2 cut(s) 103, 667
Hpy166II GTNNAC 4 cut(s) 68, 200, 358, 395
Hpy188I TCNGA 4 cut(s) 13, 420, 554, 591
Hpy188III TCNNGA 4 cut(s) 257, 413, 436, 496
Hpy8I GTNNAC 4 cut(s) 68, 200, 358, 395
Hpy99I CGWCG 1 cut(s) 433
HpyCH4III ACNGT 3 cut(s) 355, 405, 520
HpyCH4IV ACGT 2 cut(s) 202, 287
HpyCH4V TGCA 3 cut(s) 138, 233, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 627
HpyF3I CTNAG 3 cut(s) 148, 194, 279
HpySE526I ACGT 2 cut(s) 202, 287
Hsp92II CATG 1 cut(s) 61
HspAI GCGC 2 cut(s) 567, 583
Kzo9I GATC 5 cut(s) 375, 415, 432, 502, 549
LweI GCATC 3 cut(s) 180, 582, 650
MaeII ACGT 2 cut(s) 202, 287
MaeIII GTNAC 1 cut(s) 305
MalI GATC 5 cut(s) 377, 417, 434, 504, 551
MboI GATC 5 cut(s) 375, 415, 432, 502, 549
MboII GAAGA 1 cut(s) 433
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 623
MluCI AATT 1 cut(s) 536
MmeI TCCRAC 2 cut(s) 36, 464
MnlI CCTC 4 cut(s) 164, 167, 170, 506
MseI TTAA 3 cut(s) 464, 471, 558
MspI CCGG 5 cut(s) 79, 118, 245, 264, 623
MspR9I CCNGG 6 cut(s) 79, 118, 119, 246, 265, 623
MvnI CGCG 5 cut(s) 96, 102, 311, 548, 567
MwoI GCNNNNNNNGC 2 cut(s) 308, 627
NciI CCSGG 6 cut(s) 79, 118, 119, 246, 265, 623
NdeI CATATG 1 cut(s) 134
NdeII GATC 5 cut(s) 375, 415, 432, 502, 549
NlaIII CATG 1 cut(s) 61
NlaIV GGNNCC 5 cut(s) 243, 368, 417, 457, 621
NspV TTCGAA 1 cut(s) 270
PceI AGGCCT 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 2 cut(s) 260, 562
PkrI GCNGC 1 cut(s) 547
Ple19I CGATCG 1 cut(s) 435
Psp1406I AACGTT 1 cut(s) 287
PspN4I GGNNCC 5 cut(s) 243, 368, 417, 457, 621
PspOMI GGGCCC 1 cut(s) 619
PspPI GGNCC 5 cut(s) 301, 367, 456, 619, 620
PsuI RGATCY 1 cut(s) 415
PvuI CGATCG 1 cut(s) 435
RsaI GTAC 5 cut(s) 5, 32, 83, 359, 575
RsaNI GTAC 5 cut(s) 4, 31, 82, 358, 574
SaqAI TTAA 3 cut(s) 464, 471, 558
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 5 cut(s) 375, 415, 432, 502, 549
Sau96I GGNCC 5 cut(s) 301, 367, 456, 619, 620
ScrFI CCNGG 6 cut(s) 79, 118, 119, 246, 265, 623
SduI GDGCHC 1 cut(s) 623
SetI ASST 6 cut(s) 132, 205, 290, 464, 605, 680
SfaNI GCATC 3 cut(s) 180, 582, 650
SfcI CTRYAG 1 cut(s) 361
SfuI TTCGAA 1 cut(s) 270
SinI GGWCC 1 cut(s) 367
SmaI CCCGGG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 153
SsiI CCGC 6 cut(s) 75, 94, 100, 206, 211, 546
SspI AATATT 1 cut(s) 322
StuI AGGCCT 1 cut(s) 153
StyD4I CCNGG 6 cut(s) 77, 116, 117, 244, 263, 621
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 3 cut(s) 355, 405, 520
TaiI ACGT 2 cut(s) 205, 290
TaqI TCGA 1 cut(s) 270
TasI AATT 1 cut(s) 536
TatI WGTACW 2 cut(s) 3, 357
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 2 cut(s) 260, 562
Tru1I TTAA 3 cut(s) 464, 471, 558
Tru9I TTAA 3 cut(s) 464, 471, 558
TspDTI ATGAA 3 cut(s) 74, 172, 212
TspGWI ACGGA 2 cut(s) 388, 503
TspMI CCCGGG 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 1 cut(s) 536
XmaI CCCGGG 1 cut(s) 117
XmiI GTMKAC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.