MD05G1340700.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015045
Physical Location & Seq
Reverse (-)
46109596 .. 46110279
684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1340700.v1.1.491

Sequence Viewer

Length: 684 bp
ATGTACAACTCCGACCGGTTGCCGATCCAGTACTCCACCACCCCAGAGCAACAACGGCCCATGAGGCGTCAACACACCGCCCGGTACTACGCCCACCGCGTCCGCGAAAGCCTCACCACCCGGGTCTCCAAGCTCATATGCACCATTTTCTTAAGCCTCCTCCTCATCCTTGGCATGATAACGTTCATACTATGGCTCAGCCTACGTCCCCACCGACCTAGATTTCACATCCATGCATTTTCAGTTCCGGGTTTGGGTCAAGAAACCGGGTTCGCAAATGCGGGGATAATGTTCAATGCCACGGCCCGTAACGCGAACCATAATATAGGTATATATTATGATTCGATGGACGCGACGGTGTATTATAGGGACCAACGGGTTGGGTCAATCACGGGTTTACTGGACCCGTTTTATCAGGGCCCAAAGAATACGACGATCGTGATGGGGTCGTTTCAGGGGGCCACCTTAACGGTGAATAACCAGCGTTGGATGGAGTTCACGAATGATCGTAAAAAAGGAACGGTCGTGTTTCGTCTAGAATTTACGGCTACGATTAGATTTAGGATCCACGCGTGGGATAGCAGGCGTCATCGGATGCACGCCAACTGTGATGTTGATGTGGGCCAGGACGGCTTGATCTTGCCCATTTCTAAAGATAGGAGATGCCCTGTGTATTTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

228

Amino Acids

26.35

Weight (kDa)

10.16

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 101 - 203 4.3e-12 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05220
fragaria_vesca FvH4_3g02710
malus_domestica MD05G1340700.v1.1 MD10G1314200.v1.1
prunus_persica Prupe.4G026800_v2.0.a1
pyrus_communis pycom05g30990 pycom10g26620
rosa_chinensis RchiOBHm_Chr5g0004071
rosa_laevigata RLG00000031201
rosa_multiflora Rmu_sc0000547.1_g000030
rosa_rugosa Rorug04G0409800
rosa_samantha Rh5AG037300 Rh5BG036300 Rh5CG040000 Rh5DG035800
rosa_wichuraiana Rw5G003370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 99, 105, 314, 353, 572
AciI CCGC 4 cut(s) 78, 97, 103, 281
AclI AACGTT 1 cut(s) 182
AclWI GGATC 3 cut(s) 19, 559, 572
AcsI RAATTY 1 cut(s) 539
AcyI GRCGYC 2 cut(s) 67, 586
AfaI GTAC 3 cut(s) 5, 32, 86
AfiI CCNNNNNNNGG 2 cut(s) 254, 574
AflII CTTAAG 1 cut(s) 151
AflIII ACRYGT 1 cut(s) 570
AgeI ACCGGT 1 cut(s) 15
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 1 cut(s) 624
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 3 cut(s) 19, 559, 572
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 5 cut(s) 56, 303, 418, 459, 622
ApaI GGGCCC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 539
AsiGI ACCGGT 1 cut(s) 15
AspS9I GGNCC 8 cut(s) 57, 304, 370, 403, 418, 419, 459, 622
AsuC2I CCSGG 5 cut(s) 82, 121, 122, 249, 268
AsuHPI GGTGA 3 cut(s) 106, 484, 670
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 2 cut(s) 370, 403
BaeGI GKGCMC 1 cut(s) 422
BamHI GGATCC 1 cut(s) 564
BanII GRGCYC 1 cut(s) 422
BccI CCATC 3 cut(s) 340, 436, 484
BceAI ACGGC 4 cut(s) 71, 318, 561, 646
BciT130I CCWGG 1 cut(s) 626
BcnI CCSGG 5 cut(s) 82, 121, 122, 249, 268
BcoDI GTCTC 1 cut(s) 130
BfaI CTAG 2 cut(s) 219, 536
BfrI CTTAAG 1 cut(s) 151
BglI GCCNNNNNGGC 2 cut(s) 64, 630
BlpI GCTNAGC 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 32
Bme1390I CCNGG 6 cut(s) 82, 121, 122, 249, 268, 626
Bme18I GGWCC 2 cut(s) 370, 403
BmeT110I CYCGRG 1 cut(s) 120
BmgT120I GGNCC 8 cut(s) 57, 304, 370, 403, 418, 419, 459, 622
BmiI GGNNCC 5 cut(s) 371, 405, 420, 460, 566
BmrFI CCNGG 6 cut(s) 82, 121, 122, 249, 268, 626
BmsI GCATC 2 cut(s) 585, 653
Bpu1102I GCTNAGC 1 cut(s) 197
BpuMI CCSGG 5 cut(s) 82, 121, 122, 249, 268
BsaHI GRCGYC 2 cut(s) 67, 586
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 120, 169, 300
BsaWI WCCGGW 1 cut(s) 15
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 574
Bse118I RCCGGY 1 cut(s) 15
Bse1I ACTGG 2 cut(s) 28, 405
BseBI CCWGG 1 cut(s) 626
BseDI CCNNGG 3 cut(s) 120, 169, 300
BseGI GGATG 4 cut(s) 165, 228, 495, 600
BseLI CCNNNNNNNGG 2 cut(s) 254, 574
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 2 cut(s) 28, 405
BseRI GAGGAG 2 cut(s) 149, 152
BseSI GKGCMC 1 cut(s) 422
Bsh1236I CGCG 5 cut(s) 99, 105, 314, 353, 572
Bsh1285I CGRYCG 3 cut(s) 16, 438, 525
BshFI GGCC 5 cut(s) 58, 305, 420, 461, 624
BshTI ACCGGT 1 cut(s) 15
BsiEI CGRYCG 3 cut(s) 16, 438, 525
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 5 cut(s) 16, 82, 121, 248, 267
BslFI GGGAC 2 cut(s) 192, 383
BslI CCNNNNNNNGG 2 cut(s) 254, 574
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 2 cut(s) 192, 383
BsnI GGCC 5 cut(s) 58, 305, 420, 461, 624
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 120
Bsp120I GGGCCC 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 422
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 24, 435, 505, 564, 636
Bsp1720I GCTNAGC 1 cut(s) 197
BspACI CCGC 4 cut(s) 78, 97, 103, 281
BspANI GGCC 5 cut(s) 58, 305, 420, 461, 624
BspCNI CTCAG 1 cut(s) 210
BspFNI CGCG 5 cut(s) 99, 105, 314, 353, 572
BspLI GGNNCC 5 cut(s) 371, 405, 420, 460, 566
BspPI GGATC 3 cut(s) 19, 559, 572
BspTI CTTAAG 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 15
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 28, 405
BssAI RCCGGY 1 cut(s) 15
BssECI CCNNGG 3 cut(s) 120, 169, 300
BssMI GATC 5 cut(s) 24, 435, 505, 564, 636
BssNI GRCGYC 2 cut(s) 67, 586
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 4 cut(s) 358, 472, 523, 608
BstACI GRCGYC 2 cut(s) 67, 586
BstAFI CTTAAG 1 cut(s) 151
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 584, 600
BstDEI CTNAG 1 cut(s) 197
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 4 cut(s) 165, 228, 495, 600
BstFNI CGCG 5 cut(s) 99, 105, 314, 353, 572
BstKTI GATC 5 cut(s) 27, 438, 508, 567, 639
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 5 cut(s) 24, 435, 505, 564, 636
BstMCI CGRYCG 3 cut(s) 16, 438, 525
BstMWI GCNNNNNNNGC 4 cut(s) 55, 64, 311, 630
BstNI CCWGG 1 cut(s) 626
BstSCI CCNGG 6 cut(s) 80, 119, 120, 247, 266, 624
BstSLI GKGCMC 1 cut(s) 422
BstUI CGCG 5 cut(s) 99, 105, 314, 353, 572
BstX2I RGATCY 1 cut(s) 564
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 1 cut(s) 564
BsuRI GGCC 5 cut(s) 58, 305, 420, 461, 624
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 4 cut(s) 165, 228, 495, 600
Cac8I GCNNGC 2 cut(s) 584, 600
Cfr10I RCCGGY 1 cut(s) 15
Cfr13I GGNCC 8 cut(s) 57, 304, 370, 403, 418, 419, 459, 622
Cfr9I CCCGGG 1 cut(s) 120
CseI GACGC 4 cut(s) 56, 88, 359, 575
Csp6I GTAC 3 cut(s) 4, 31, 85
CspAI ACCGGT 1 cut(s) 15
CviAII CATG 3 cut(s) 61, 175, 233
CviQI GTAC 3 cut(s) 4, 31, 85
DdeI CTNAG 1 cut(s) 197
DpnI GATC 5 cut(s) 26, 437, 507, 566, 638
DpnII GATC 5 cut(s) 24, 435, 505, 564, 636
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 422
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 370, 403
Eco88I CYCGRG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 624
EcoT14I CCWWGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 3 cut(s) 64, 178, 236
FaqI GGGAC 2 cut(s) 192, 383
FatI CATG 3 cut(s) 60, 174, 232
FauI CCCGC 1 cut(s) 274
FauNDI CATATG 1 cut(s) 137
FokI GGATG 4 cut(s) 152, 215, 502, 607
FriOI GRGCYC 1 cut(s) 422
FspBI CTAG 2 cut(s) 219, 536
HaeIII GGCC 5 cut(s) 58, 305, 420, 461, 624
HapII CCGG 5 cut(s) 16, 82, 121, 248, 267
HgaI GACGC 4 cut(s) 56, 88, 359, 575
Hin1I GRCGYC 2 cut(s) 67, 586
Hin1II CATG 3 cut(s) 64, 178, 236
HincII GTYRAC 1 cut(s) 71
HindII GTYRAC 1 cut(s) 71
HinfI GANTC 1 cut(s) 341
HpaII CCGG 5 cut(s) 16, 82, 121, 248, 267
HphI GGTGA 3 cut(s) 106, 484, 670
Hpy166II GTNNAC 3 cut(s) 71, 398, 498
Hpy188I TCNGA 2 cut(s) 13, 594
Hpy188III TCNNGA 4 cut(s) 260, 439, 499, 536
Hpy8I GTNNAC 3 cut(s) 71, 398, 498
Hpy99I CGWCG 2 cut(s) 358, 436
HpyCH4III ACNGT 4 cut(s) 358, 472, 523, 608
HpyCH4IV ACGT 2 cut(s) 182, 205
HpyCH4V TGCA 3 cut(s) 141, 236, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 64, 311, 630
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 2 cut(s) 182, 205
Hsp92I GRCGYC 2 cut(s) 67, 586
Hsp92II CATG 3 cut(s) 64, 178, 236
Kzo9I GATC 5 cut(s) 24, 435, 505, 564, 636
LweI GCATC 2 cut(s) 585, 653
MaeI CTAG 2 cut(s) 219, 536
MaeII ACGT 2 cut(s) 182, 205
MaeIII GTNAC 1 cut(s) 308
MalI GATC 5 cut(s) 26, 437, 507, 566, 638
MboI GATC 5 cut(s) 24, 435, 505, 564, 636
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 422
MluCI AATT 1 cut(s) 539
MluI ACGCGT 1 cut(s) 570
MmeI TCCRAC 2 cut(s) 36, 467
MnlI CCTC 5 cut(s) 57, 122, 167, 170, 173
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 2 cut(s) 152, 467
MslI CAYNNNNRTG 1 cut(s) 231
MspCI CTTAAG 1 cut(s) 151
MspI CCGG 5 cut(s) 16, 82, 121, 248, 267
MspR9I CCNGG 6 cut(s) 82, 121, 122, 249, 268, 626
MvaI CCWGG 1 cut(s) 626
MvnI CGCG 5 cut(s) 99, 105, 314, 353, 572
MwoI GCNNNNNNNGC 4 cut(s) 55, 64, 311, 630
NciI CCSGG 5 cut(s) 82, 121, 122, 249, 268
NdeI CATATG 1 cut(s) 137
NdeII GATC 5 cut(s) 24, 435, 505, 564, 636
NlaIII CATG 3 cut(s) 64, 178, 236
NlaIV GGNNCC 5 cut(s) 371, 405, 420, 460, 566
NsiI ATGCAT 1 cut(s) 238
PcsI WCGNNNNNNNCGW 3 cut(s) 96, 211, 350
PfeI GAWTC 1 cut(s) 341
PinAI ACCGGT 1 cut(s) 15
Ple19I CGATCG 1 cut(s) 438
Psp1406I AACGTT 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 624
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 5 cut(s) 371, 405, 420, 460, 566
PspOMI GGGCCC 1 cut(s) 418
PspPI GGNCC 8 cut(s) 57, 304, 370, 403, 418, 419, 459, 622
PsuI RGATCY 1 cut(s) 564
PvuI CGATCG 1 cut(s) 438
RsaI GTAC 3 cut(s) 5, 32, 86
RsaNI GTAC 3 cut(s) 4, 31, 85
RseI CAYNNNNRTG 1 cut(s) 231
SaqAI TTAA 2 cut(s) 152, 467
Sau3AI GATC 5 cut(s) 24, 435, 505, 564, 636
Sau96I GGNCC 8 cut(s) 57, 304, 370, 403, 418, 419, 459, 622
ScaI AGTACT 1 cut(s) 32
ScrFI CCNGG 6 cut(s) 82, 121, 122, 249, 268, 626
SduI GDGCHC 1 cut(s) 422
SetI ASST 7 cut(s) 135, 185, 208, 220, 331, 467, 683
SfaNI GCATC 2 cut(s) 585, 653
SinI GGWCC 2 cut(s) 370, 403
SmaI CCCGGG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 1 cut(s) 539
SsiI CCGC 4 cut(s) 78, 97, 103, 281
SspMI CTAG 2 cut(s) 219, 536
StyD4I CCNGG 6 cut(s) 80, 119, 120, 247, 266, 624
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 4 cut(s) 358, 472, 523, 608
TaiI ACGT 2 cut(s) 185, 208
TaqI TCGA 1 cut(s) 344
TasI AATT 1 cut(s) 539
TatI WGTACW 2 cut(s) 3, 30
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 2 cut(s) 152, 467
Tru9I TTAA 2 cut(s) 152, 467
TspDTI ATGAA 1 cut(s) 175
TspMI CCCGGG 1 cut(s) 120
Vha464I CTTAAG 1 cut(s) 151
VpaK11BI GGWCC 2 cut(s) 370, 403
XapI RAATTY 1 cut(s) 539
XbaI TCTAGA 1 cut(s) 535
XmaI CCCGGG 1 cut(s) 120
XspI CTAG 2 cut(s) 219, 536
ZrmI AGTACT 1 cut(s) 32
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.