AT4G05350

zinc-RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018729
Physical Location & Seq
Reverse (-)
2726910 .. 2727530
621 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05350.1

Sequence Viewer

Length: 621 bp
ATGAGTATGAGTTCTCGTCCTGAAGTGGAAGTTCACGTCACCAACGGAAGGAAACTCTCTCCATCAGAACCAAACTCAGTCATCATACGCCTCAAAACCAAATCTGTAGAGATCATTATTGAAAACCAAACAACCGGTCGCCAAAACCGCCGGACTCAGGTTTCACCACCTTTTCTCATCGATATCAATCTCCGTAGCTCTAGTCACGACCACATCCGAACGCTTCTCCACGATCGATTAGTCGGCTACCCCTTTGAGAGTTACTTGTGCGATGACTTAGCCCCAAAGATCTCAACGGCTGCAATAGACTTAGGTTTTGGGCACAACGGCTTTACCTTGACCATGGCCGCTACAGTTACTTACCGGACCGTCTCAGTTATGTCAAACGACGAGAAATCATTGAGGATGGTTCTGCTAGGGAGAATGAAAGCAGAGGAATTCAAGAGTTTGAAGATGGAAACAGAGCCGTGTTCCATATGTCTCGAGAGTCTAGTCTCCGGTCCGAAACCTAGGGACGTCACACGCATGACTTGTTCTCATGTCTTCCACAATGGCTGTCTTTTGGAGTGGCTCAAGCGTAAGAACACTTGTCCCCTGTGTCGTACTGAGATCTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

206

Amino Acids

23.31

Weight (kDa)

8.69

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 154 - 204 7.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 157 - 201 1.5e-13 Ring finger domain
zf-C3HC4_2 PF13923 157 - 200 2.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 157 - 200 4.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 157 - 198 1e-06 RING-type zinc-finger
zf-RING_11 PF17123 157 - 187 1.4e-06 RING-like zinc finger
zf-RING_5 PF14634 157 - 202 1.2e-06 zinc-RING finger domain
zf-RING_Vps41 PF23555 157 - 194 9.2e-06 Vps41 C-terminal RING finger domain
zf-rbx1 PF12678 172 - 201 1.2e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05350 AT4G12140 AT4G12150 AT4G12190 AT4G12210 AT4G12220 AT4G18110
rosa_chinensis RchiOBHm_Chr4g0400421 RchiOBHm_Chr4g0400491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 519
AciI CCGC 2 cut(s) 148, 348
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 2 cut(s) 48, 157
AgeI ACCGGT 1 cut(s) 134
AgsI TTSAA 3 cut(s) 122, 442, 451
AjiI CACGTC 1 cut(s) 37
AluBI AGCT 1 cut(s) 198
AluI AGCT 1 cut(s) 198
Alw26I GTCTC 3 cut(s) 376, 485, 499
Ama87I CYCGRG 1 cut(s) 482
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 1 cut(s) 437
ArsI GACNNNNNNTTYG 4 cut(s) 121, 153, 299, 331
AsiGI ACCGGT 1 cut(s) 134
AspA2I CCTAGG 1 cut(s) 509
AspS9I GGNCC 2 cut(s) 366, 500
AsuHPI GGTGA 2 cut(s) 31, 156
AvaI CYCGRG 1 cut(s) 482
AvaII GGWCC 2 cut(s) 366, 500
AvrII CCTAGG 1 cut(s) 509
BaeGI GKGCMC 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 286
BccI CCATC 3 cut(s) 70, 400, 448
BceAI ACGGC 3 cut(s) 312, 343, 451
BcoDI GTCTC 3 cut(s) 376, 485, 499
BfaI CTAG 4 cut(s) 201, 416, 491, 510
BfmI CTRYAG 2 cut(s) 105, 351
BglII AGATCT 2 cut(s) 288, 609
BisI GCNGC 2 cut(s) 300, 348
BlnI CCTAGG 1 cut(s) 509
BlsI GCNGC 2 cut(s) 301, 349
Bme18I GGWCC 2 cut(s) 366, 500
BmeT110I CYCGRG 1 cut(s) 482
BmgBI CACGTC 1 cut(s) 37
BmgT120I GGNCC 2 cut(s) 366, 500
BpiI GAAGAC 1 cut(s) 535
BpuEI CTTGAG 1 cut(s) 557
Bsa29I ATCGAT 2 cut(s) 180, 235
BsaBI GATNNNNATC 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 2 cut(s) 342, 509
BsaWI WCCGGW 3 cut(s) 134, 363, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 157
Bse118I RCCGGY 1 cut(s) 134
Bse8I GATNNNNATC 1 cut(s) 186
BseCI ATCGAT 2 cut(s) 180, 235
BseDI CCNNGG 2 cut(s) 342, 509
BseGI GGATG 2 cut(s) 213, 411
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 2 cut(s) 48, 157
BseMII CTCAG 4 cut(s) 90, 170, 387, 597
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 1 cut(s) 286
Bsh1285I CGRYCG 2 cut(s) 139, 235
BshFI GGCC 1 cut(s) 347
BshTI ACCGGT 1 cut(s) 134
BshVI ATCGAT 2 cut(s) 180, 235
BsiEI CGRYCG 2 cut(s) 139, 235
BsiHKCI CYCGRG 1 cut(s) 482
BsiSI CCGG 4 cut(s) 135, 151, 364, 498
BslFI GGGAC 2 cut(s) 527, 576
BslI CCNNNNNNNGG 2 cut(s) 48, 157
BsmAI GTCTC 3 cut(s) 376, 485, 499
BsmBI CGTCTC 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 527, 576
BsnI GGCC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 482
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 4 cut(s) 111, 232, 288, 609
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 2 cut(s) 148, 348
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 4 cut(s) 89, 169, 386, 598
BspDI ATCGAT 2 cut(s) 180, 235
BsrFI RCCGGY 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 2 cut(s) 342, 509
BssMI GATC 4 cut(s) 111, 232, 288, 609
BssNI GRCGYC 1 cut(s) 516
BssT1I CCWWGG 2 cut(s) 342, 509
Bst4CI ACNGT 2 cut(s) 355, 370
BstACI GRCGYC 1 cut(s) 516
BstDEI CTNAG 6 cut(s) 76, 156, 277, 310, 373, 606
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 2 cut(s) 213, 411
BstKTI GATC 4 cut(s) 114, 235, 291, 612
BstMAI GTCTC 3 cut(s) 376, 485, 499
BstMBI GATC 4 cut(s) 111, 232, 288, 609
BstMCI CGRYCG 2 cut(s) 139, 235
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstSFI CTRYAG 2 cut(s) 105, 351
BstSLI GKGCMC 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 535
BstX2I RGATCY 2 cut(s) 288, 609
BstYI RGATCY 2 cut(s) 288, 609
Bsu15I ATCGAT 2 cut(s) 180, 235
BsuRI GGCC 1 cut(s) 347
BsuTUI ATCGAT 2 cut(s) 180, 235
BtgI CCRYGG 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 285
BtrI CACGTC 1 cut(s) 37
BtsCI GGATG 2 cut(s) 213, 411
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 366, 500
ClaI ATCGAT 2 cut(s) 180, 235
CpoI CGGWCCG 2 cut(s) 366, 500
Csp6I GTAC 1 cut(s) 603
CspAI ACCGGT 1 cut(s) 134
CspI CGGWCCG 2 cut(s) 366, 500
CviAII CATG 3 cut(s) 343, 526, 539
CviJI RGCY 9 cut(s) 198, 246, 281, 299, 330, 347, 466, 555, 571
CviKI_1 RGCY 9 cut(s) 198, 246, 281, 299, 330, 347, 466, 555, 571
CviQI GTAC 1 cut(s) 603
DdeI CTNAG 6 cut(s) 76, 156, 277, 310, 373, 606
DpnI GATC 4 cut(s) 113, 234, 290, 611
DpnII GATC 4 cut(s) 111, 232, 288, 609
EaeI YGGCCR 1 cut(s) 345
Eco130I CCWWGG 2 cut(s) 342, 509
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 366, 500
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 482
EcoRI GAATTC 1 cut(s) 437
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 2 cut(s) 342, 509
ErhI CCWWGG 2 cut(s) 342, 509
Esp3I CGTCTC 1 cut(s) 376
FaeI CATG 3 cut(s) 346, 529, 542
FaiI YATR 8 cut(s) 8, 86, 344, 380, 476, 478, 527, 540
FaqI GGGAC 2 cut(s) 527, 576
FatI CATG 3 cut(s) 342, 525, 538
FauNDI CATATG 1 cut(s) 476
Fnu4HI GCNGC 2 cut(s) 300, 348
FokI GGATG 2 cut(s) 200, 418
Fsp4HI GCNGC 2 cut(s) 300, 348
FspBI CTAG 4 cut(s) 201, 416, 491, 510
GluI GCNGC 2 cut(s) 300, 348
HaeIII GGCC 1 cut(s) 347
HapII CCGG 4 cut(s) 135, 151, 364, 498
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 3 cut(s) 346, 529, 542
HinfI GANTC 2 cut(s) 154, 487
HpaII CCGG 4 cut(s) 135, 151, 364, 498
HphI GGTGA 2 cut(s) 31, 156
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 3 cut(s) 67, 218, 504
Hpy188III TCNNGA 5 cut(s) 20, 206, 442, 482, 484
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 42
HpyCH4III ACNGT 2 cut(s) 355, 370
HpyCH4IV ACGT 2 cut(s) 36, 516
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpyF3I CTNAG 6 cut(s) 76, 156, 277, 310, 373, 606
HpySE526I ACGT 2 cut(s) 36, 516
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 3 cut(s) 346, 529, 542
Kzo9I GATC 4 cut(s) 111, 232, 288, 609
LpnPI CCDG 7 cut(s) 33, 143, 148, 164, 377, 511, 608
Lsp1109I GCAGC 1 cut(s) 286
MaeI CTAG 4 cut(s) 201, 416, 491, 510
MaeII ACGT 2 cut(s) 36, 516
MaeIII GTNAC 5 cut(s) 37, 203, 260, 355, 517
MalI GATC 4 cut(s) 113, 234, 290, 611
MboI GATC 4 cut(s) 111, 232, 288, 609
MboII GAAGA 2 cut(s) 463, 535
MflI RGATCY 2 cut(s) 288, 609
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 1 cut(s) 437
MlyI GAGTC 2 cut(s) 148, 496
MnlI CCTC 3 cut(s) 101, 396, 427
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 4 cut(s) 135, 151, 364, 498
MwoI GCNNNNNNNGC 1 cut(s) 147
NcoI CCATGG 1 cut(s) 342
NdeI CATATG 1 cut(s) 476
NdeII GATC 4 cut(s) 111, 232, 288, 609
NlaIII CATG 3 cut(s) 346, 529, 542
NmuCI GTSAC 3 cut(s) 37, 203, 517
PaeR7I CTCGAG 1 cut(s) 482
PinAI ACCGGT 1 cut(s) 134
PkrI GCNGC 2 cut(s) 301, 349
Ple19I CGATCG 1 cut(s) 235
PleI GAGTC 2 cut(s) 148, 495
PpsI GAGTC 2 cut(s) 148, 495
PspPI GGNCC 2 cut(s) 366, 500
PsuI RGATCY 2 cut(s) 288, 609
PvuI CGATCG 1 cut(s) 235
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
RseI CAYNNNNRTG 1 cut(s) 524
Rsr2I CGGWCCG 2 cut(s) 366, 500
RsrII CGGWCCG 2 cut(s) 366, 500
SatI GCNGC 2 cut(s) 300, 348
Sau3AI GATC 4 cut(s) 111, 232, 288, 609
Sau96I GGNCC 2 cut(s) 366, 500
SchI GAGTC 2 cut(s) 148, 496
SduI GDGCHC 1 cut(s) 324
SetI ASST 8 cut(s) 39, 162, 172, 200, 316, 338, 511, 519
SfcI CTRYAG 2 cut(s) 105, 351
Sfr274I CTCGAG 1 cut(s) 482
SinI GGWCC 2 cut(s) 366, 500
SlaI CTCGAG 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 2 cut(s) 482, 572
SmoI CTYRAG 2 cut(s) 482, 572
Sse9I AATT 1 cut(s) 437
SsiI CCGC 2 cut(s) 148, 348
SspMI CTAG 4 cut(s) 201, 416, 491, 510
StyI CCWWGG 2 cut(s) 342, 509
TaaI ACNGT 2 cut(s) 355, 370
TaiI ACGT 2 cut(s) 39, 519
TaqI TCGA 3 cut(s) 180, 235, 483
TasI AATT 1 cut(s) 437
TauI GCSGC 1 cut(s) 350
TseFI GTSAC 3 cut(s) 37, 203, 517
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 3 cut(s) 37, 203, 517
TspDTI ATGAA 1 cut(s) 440
TspGWI ACGGA 2 cut(s) 60, 182
VpaK11BI GGWCC 2 cut(s) 366, 500
XapI RAATTY 1 cut(s) 437
XhoI CTCGAG 1 cut(s) 482
XmaJI CCTAGG 1 cut(s) 509
XspI CTAG 4 cut(s) 201, 416, 491, 510
ZraI GACGTC 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.