AT4G12220

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018729
Physical Location & Seq
Reverse (-)
7283248 .. 7283739
492 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12220.1

Sequence Viewer

Length: 492 bp
ATGAAATATTGCAAAGTGGAATTACGCTTCATCAACCAAAGGAAACTCTCTCCATCACAACCAAACTCAGTTCTCGTTTTCATCGAAACCAAATCTGAAGAGATCTTTGTAAACCCAACAACCGGTCACCAAACCAGCCGGTCCACTCCGGCTTCACCATCTTTTTTCGATACTATCAATCTCTGTAGTTCTAGTCACCACCACATCCGATCACTTCTCCGTAGTCGGTTCGTTAGCTACCATTTTTTGTGTGATATCTTAGCCCCAGAGATCGCGACGGAGGCAATCAACTTAGGTTTTGGACGAAACGGCTTTACCTTCACCGTGGTCGTCACAGTTACTAACCGGACCGTCTCCGTTACTTCAAACGACACAGAAATCATTGAGAATGGTTCTACTAGAGAGGATAAAAGCAGAGGAATTAAAGAGTTCGAACATGGAAACAGAGTCGTGTTCCATCTGTTTCCACGATCTCTCCAGTCCGAAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

163

Amino Acids

18.47

Weight (kDa)

8.54

Isoelectric Point (pI)

60.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05350 AT4G12140 AT4G12150 AT4G12190 AT4G12210 AT4G12220 AT4G18110
rosa_chinensis RchiOBHm_Chr4g0400421 RchiOBHm_Chr4g0400491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 117
AfiI CCNNNNNNNGG 1 cut(s) 122
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 1 cut(s) 366
AluBI AGCT 1 cut(s) 237
AluI AGCT 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 358
AsiGI ACCGGT 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 141, 348
AsuHPI GGTGA 4 cut(s) 119, 147, 188, 313
AsuII TTCGAA 1 cut(s) 432
AvaII GGWCC 2 cut(s) 141, 348
BccI CCATC 3 cut(s) 61, 166, 465
BceAI ACGGC 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 3 cut(s) 192, 399, 490
BfmI CTRYAG 1 cut(s) 184
BglII AGATCT 1 cut(s) 102
Bme18I GGWCC 2 cut(s) 141, 348
BmgT120I GGNCC 2 cut(s) 141, 348
BpmI CTGGAG 1 cut(s) 461
Bpu14I TTCGAA 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 2 cut(s) 122, 345
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse118I RCCGGY 2 cut(s) 122, 138
Bse1I ACTGG 1 cut(s) 478
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 275
BshTI ACCGGT 1 cut(s) 122
BsiSI CCGG 4 cut(s) 123, 139, 149, 346
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 358
BsmBI CGTCTC 1 cut(s) 358
Bsp119I TTCGAA 1 cut(s) 432
Bsp143I GATC 4 cut(s) 102, 209, 270, 470
Bsp68I TCGCGA 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 80
BspFNI CGCG 1 cut(s) 275
BspT104I TTCGAA 1 cut(s) 432
BsrFI RCCGGY 2 cut(s) 122, 138
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 2 cut(s) 122, 138
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 4 cut(s) 102, 209, 270, 470
Bst4CI ACNGT 3 cut(s) 325, 337, 352
Bst6I CTCTTC 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 432
BstDEI CTNAG 3 cut(s) 67, 259, 292
BstDSI CCRYGG 1 cut(s) 324
BstEII GGTNACC 1 cut(s) 125
BstF5I GGATG 1 cut(s) 204
BstFNI CGCG 1 cut(s) 275
BstKTI GATC 4 cut(s) 105, 212, 273, 473
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 4 cut(s) 102, 209, 270, 470
BstMWI GCNNNNNNNGC 1 cut(s) 281
BstPI GGTNACC 1 cut(s) 125
BstSFI CTRYAG 1 cut(s) 184
BstUI CGCG 1 cut(s) 275
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 1 cut(s) 204
BtuMI TCGCGA 1 cut(s) 275
Cfr10I RCCGGY 2 cut(s) 122, 138
Cfr13I GGNCC 2 cut(s) 141, 348
CpoI CGGWCCG 1 cut(s) 348
CspAI ACCGGT 1 cut(s) 122
CspI CGGWCCG 1 cut(s) 348
CviAII CATG 1 cut(s) 437
CviJI RGCY 5 cut(s) 138, 152, 237, 263, 312
CviKI_1 RGCY 5 cut(s) 138, 152, 237, 263, 312
DdeI CTNAG 3 cut(s) 67, 259, 292
DpnI GATC 4 cut(s) 104, 211, 272, 472
DpnII GATC 4 cut(s) 102, 209, 270, 470
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 2 cut(s) 141, 348
Eco57I CTGAAG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 125
EcoO65I GGTNACC 1 cut(s) 125
EcoRV GATATC 1 cut(s) 256
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 1 cut(s) 440
FaiI YATR 1 cut(s) 438
FatI CATG 1 cut(s) 436
FokI GGATG 1 cut(s) 191
FspBI CTAG 3 cut(s) 192, 399, 490
GsuI CTGGAG 1 cut(s) 461
HapII CCGG 4 cut(s) 123, 139, 149, 346
Hin1II CATG 1 cut(s) 440
HinfI GANTC 1 cut(s) 447
HpaII CCGG 4 cut(s) 123, 139, 149, 346
HphI GGTGA 4 cut(s) 119, 147, 188, 313
Hpy166II GTNNAC 2 cut(s) 112, 144
Hpy188I TCNGA 3 cut(s) 97, 209, 484
Hpy188III TCNNGA 1 cut(s) 274
Hpy8I GTNNAC 2 cut(s) 112, 144
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 3 cut(s) 325, 337, 352
HpyCH4V TGCA 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 1 cut(s) 281
HpyF3I CTNAG 3 cut(s) 67, 259, 292
Hsp92II CATG 1 cut(s) 440
Kzo9I GATC 4 cut(s) 102, 209, 270, 470
LpnPI CCDG 6 cut(s) 136, 148, 152, 162, 279, 359
MaeI CTAG 3 cut(s) 192, 399, 490
MaeIII GTNAC 5 cut(s) 125, 194, 331, 337, 358
MalI GATC 4 cut(s) 104, 211, 272, 472
MboI GATC 4 cut(s) 102, 209, 270, 470
MboII GAAGA 1 cut(s) 110
MflI RGATCY 1 cut(s) 102
MluCI AATT 2 cut(s) 20, 420
MlyI GAGTC 1 cut(s) 456
MnlI CCTC 3 cut(s) 274, 397, 410
MseI TTAA 1 cut(s) 423
MspI CCGG 4 cut(s) 123, 139, 149, 346
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 1 cut(s) 281
NdeII GATC 4 cut(s) 102, 209, 270, 470
NlaIII CATG 1 cut(s) 440
NmuCI GTSAC 3 cut(s) 125, 194, 331
NruI TCGCGA 1 cut(s) 275
NspV TTCGAA 1 cut(s) 432
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PinAI ACCGGT 1 cut(s) 122
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
PspEI GGTNACC 1 cut(s) 125
PspPI GGNCC 2 cut(s) 141, 348
PsuI RGATCY 1 cut(s) 102
RruI TCGCGA 1 cut(s) 275
Rsr2I CGGWCCG 1 cut(s) 348
RsrII CGGWCCG 1 cut(s) 348
SaqAI TTAA 1 cut(s) 423
Sau3AI GATC 4 cut(s) 102, 209, 270, 470
Sau96I GGNCC 2 cut(s) 141, 348
SchI GAGTC 1 cut(s) 456
SetI ASST 4 cut(s) 239, 298, 320, 491
SfcI CTRYAG 1 cut(s) 184
SfuI TTCGAA 1 cut(s) 432
SinI GGWCC 2 cut(s) 141, 348
Sse9I AATT 2 cut(s) 20, 420
SspI AATATT 1 cut(s) 8
SspMI CTAG 3 cut(s) 192, 399, 490
TaaI ACNGT 3 cut(s) 325, 337, 352
TaqI TCGA 3 cut(s) 84, 168, 432
TasI AATT 2 cut(s) 20, 420
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseFI GTSAC 3 cut(s) 125, 194, 331
Tsp45I GTSAC 3 cut(s) 125, 194, 331
TspDTI ATGAA 3 cut(s) 17, 19, 70
TspGWI ACGGA 3 cut(s) 209, 293, 346
VpaK11BI GGWCC 2 cut(s) 141, 348
XspI CTAG 3 cut(s) 192, 399, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.