RchiOBHm_Chr4g0400421

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018729
Physical Location & Seq
Reverse (-)
18004337 .. 18004753
417 bp
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UTR
Exon/CDS
Intron
PRQ37246

Sequence Viewer

Length: 417 bp
ATGAGACATGATAGCTTGGAAGAAGAAACTAGAACGTGTGTTGGTGTTGGTGTCGGTGAAGGTGGTGTCAGTGACGTCGGTGAGGAGGACGAGGACGACAATGACGATGTGGTTGAGACAAGTCCTGCAAGTGAATCAGCCATAGAGGAGTTGGAGGAAATGAGAGATGATAGCTTGGAAGAAGAAACTAGAACATGTGTTATATGTCTTGAGGAGGTTGCTGGAAAAGCTACTCGGTTGCCTTGCTTACACCTTTTTCATGAAGCTTGTATTGAAGAGTGGTTAAAGATCAAACATATGTGTCCCTTGTGCCGTTACCCAATACCTTGCGATGCAAACCAAGGTAGTTTGTGGTGGTTTTGCCTTTTAAGTTTCTTTCGACTTTTTTTCAATTACCACCTGCATACTATATATTAA

Protein Analysis

138

Amino Acids

15.83

Weight (kDa)

4.35

Isoelectric Point (pI)

56.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 65 - 105 1.9e-12 Ring finger domain
zf-rbx1 PF12678 65 - 105 8.7e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 66 - 104 7.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 66 - 104 1.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 66 - 91 7.6e-07 RING-like zinc finger
zf-RING_UBOX PF13445 66 - 102 5.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05350 AT4G12140 AT4G12150 AT4G12190 AT4G12210 AT4G12220 AT4G18110
rosa_chinensis RchiOBHm_Chr4g0400421 RchiOBHm_Chr4g0400491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 408
AatII GACGTC 1 cut(s) 78
Acc36I ACCTGC 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 75
AflIII ACRYGT 2 cut(s) 35, 194
AgsI TTSAA 2 cut(s) 275, 391
AluBI AGCT 4 cut(s) 15, 174, 230, 266
AluI AGCT 4 cut(s) 15, 174, 230, 266
Alw26I GTCTC 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 68, 92
BceAI ACGGC 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 2 cut(s) 30, 189
BfuAI ACCTGC 1 cut(s) 408
BmsI GCATC 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 340
BseRI GAGGAG 3 cut(s) 98, 161, 227
BslFI GGGAC 1 cut(s) 288
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 288
Bsp143I GATC 1 cut(s) 288
BspHI TCATGA 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 1 cut(s) 288
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 340
Bst6I CTCTTC 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 75
BstKTI GATC 1 cut(s) 291
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 288
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 76
BveI ACCTGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 259
CviAII CATG 3 cut(s) 8, 195, 260
CviJI RGCY 5 cut(s) 15, 140, 174, 230, 266
CviKI_1 RGCY 5 cut(s) 15, 140, 174, 230, 266
DpnI GATC 1 cut(s) 290
DpnII GATC 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 3 cut(s) 11, 198, 263
FaqI GGGAC 1 cut(s) 288
FatI CATG 3 cut(s) 7, 194, 259
FauNDI CATATG 1 cut(s) 297
FspBI CTAG 2 cut(s) 30, 189
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 3 cut(s) 11, 198, 263
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 1 cut(s) 134
HphI GGTGA 2 cut(s) 68, 92
Hpy188III TCNNGA 2 cut(s) 209, 260
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 53
HpyCH4IV ACGT 2 cut(s) 35, 75
HpyCH4V TGCA 3 cut(s) 128, 335, 403
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpySE526I ACGT 2 cut(s) 35, 75
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 3 cut(s) 11, 198, 263
Kzo9I GATC 1 cut(s) 288
LpnPI CCDG 3 cut(s) 138, 207, 413
LweI GCATC 1 cut(s) 322
MaeI CTAG 2 cut(s) 30, 189
MaeII ACGT 2 cut(s) 35, 75
MaeIII GTNAC 2 cut(s) 71, 314
MalI GATC 1 cut(s) 290
MboI GATC 1 cut(s) 288
MboII GAAGA 5 cut(s) 32, 35, 191, 194, 287
MluCI AATT 1 cut(s) 391
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 7 cut(s) 76, 79, 85, 139, 148, 205, 208
MseI TTAA 3 cut(s) 284, 368, 415
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeI CATATG 1 cut(s) 297
NdeII GATC 1 cut(s) 288
NlaIII CATG 3 cut(s) 11, 198, 263
NmuCI GTSAC 1 cut(s) 71
NspI RCATGY 1 cut(s) 198
PagI TCATGA 1 cut(s) 259
PaqCI CACCTGC 1 cut(s) 408
PciI ACATGT 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 1 cut(s) 134
PscI ACATGT 1 cut(s) 194
SaqAI TTAA 3 cut(s) 284, 368, 415
Sau3AI GATC 1 cut(s) 288
SfaNI GCATC 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 391
SspMI CTAG 2 cut(s) 30, 189
StyI CCWWGG 1 cut(s) 340
TaiI ACGT 2 cut(s) 38, 78
TaqI TCGA 1 cut(s) 379
TasI AATT 1 cut(s) 391
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 3 cut(s) 284, 368, 415
Tru9I TTAA 3 cut(s) 284, 368, 415
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 2 cut(s) 248, 276
TspRI CASTG 1 cut(s) 76
XceI RCATGY 1 cut(s) 198
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XspI CTAG 2 cut(s) 30, 189
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.