AT4G12150

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018729
Physical Location & Seq
Reverse (-)
7268837 .. 7270267
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12150.1

Sequence Viewer

Length: 1074 bp
ATGAATGATCCCATTCCCAAAGCCGTAGTGAGCGTTCAGGTCCAAGCTGAGAGAGTCTCTCGCTCTCCATCACAAGGAAATACAAACTCGATCTTCCTCATCGTCAATACCATTAAGGATGAGATCCTTATAAACCCTGGCACTGGCCATCGAACCGCAACGTCCACTCCGCTTTCCTATAAATCACTTCCCATCAATTACACTCTCCCTAGTTGTAGTCACCACCACATCCAATCGTTACTCCATGATCGACTCCATAGAGACGACCATTGGTTGTGCGATCACTTAGTCCCAAAGATCTCAAGTGCTATCAGCTCAGGTTTTGTTGACTTGACCGTGAGCGTCGCGGTTACTTACAAGTACGTCCGTGTCGACGAGGCAGCATTAAAGATATCGAGGATGGTTCTACAAGGGTTCATGAGCGTAGAGGAAACAAAGAGTTTGAACGCAGAGTCGTGTTCCATTTGTCTCCAGAGTCTAGTCTCCAGTTCGAAAACAGCACCCACTCGCATGAGTTGTTCCCATGTCTTCCACAATGGTTGTCTCGTGGAGTGGCTAAACCGCAAAAACACTTGCCCGATGTTTCACATGCAACTTATAGACTCATCAATGTTTTCCTCAACAGACTACACAGCTTATATTGTAGAACATTGCAAAAGACATGACTTCTCCTCAAGTGGAAGTACACATGTCCGTTCACGTTATATCAATATTTGTCGTTCTACTCACGGCCACATCCGAAAGCTTCTCCACAATCAATTCCTATCCACAATCAGCAACCCTTGGCGTTCCTTGTGCGATTTCTTATCCCCAAAGATCTCAACGGAGTTTATCAACTTAGGTTTTGGACGCAACGGCTTTACCTTGACCATGGACGCTAAAGTTACTTACCGGACCGTCTCCGTTACGTCAAACGACGAGAAATCATTGAGGACAATTCTAGTCGGGAAAATAAAAGCAGAGGAATTGAAGAGTTTGAAGATCGAAACAGAGCGGTGTTCCATCTGTCTCGAGAGTCTAGTCTCCGGTCCGAAACCTATCGGCTTAACACGAATGCCATGTTTCATCTATTGA

Protein Analysis

357

Amino Acids

40.03

Weight (kDa)

9.0

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 152 - 194 5.4e-11 Ring finger domain
zf-C3HC4_2 PF13923 153 - 194 3.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 153 - 194 3.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 153 - 182 5.5e-06 RING-like zinc finger
zf-RING_UBOX PF13445 153 - 193 6.5e-06 RING-type zinc-finger
zf-rbx1 PF12678 156 - 194 2.1e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05350 AT4G12140 AT4G12150 AT4G12190 AT4G12210 AT4G12220 AT4G18110
rosa_chinensis RchiOBHm_Chr4g0400421 RchiOBHm_Chr4g0400491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AccBSI CCGCTC 1 cut(s) 994
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 347
AciI CCGC 5 cut(s) 156, 170, 347, 562, 994
AclWI GGATC 2 cut(s) 2, 118
AcoI YGGCCR 2 cut(s) 145, 730
AfaI GTAC 2 cut(s) 362, 685
AfiI CCNNNNNNNGG 2 cut(s) 74, 143
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 3 cut(s) 445, 970, 979
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 4 cut(s) 47, 315, 635, 745
AluI AGCT 4 cut(s) 47, 315, 635, 745
Alw26I GTCTC 8 cut(s) 61, 255, 473, 487, 548, 904, 1013, 1027
AlwI GGATC 2 cut(s) 2, 118
Ama87I CYCGRG 1 cut(s) 1010
AoxI GGCC 2 cut(s) 145, 730
ApeKI GCWGC 1 cut(s) 380
AspS9I GGNCC 3 cut(s) 40, 894, 1028
AsuHPI GGTGA 1 cut(s) 212
AsuII TTCGAA 1 cut(s) 491
AvaI CYCGRG 1 cut(s) 1010
AvaII GGWCC 3 cut(s) 40, 894, 1028
BalI TGGCCA 1 cut(s) 147
BauI CACGAG 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 520
BbvI GCAGC 1 cut(s) 392
BccI CCATC 5 cut(s) 76, 156, 200, 394, 1010
BceAI ACGGC 3 cut(s) 8, 745, 871
BciT130I CCWGG 1 cut(s) 138
BcoDI GTCTC 8 cut(s) 61, 255, 473, 487, 548, 904, 1013, 1027
BfaI CTAG 4 cut(s) 210, 479, 941, 1019
BglII AGATCT 2 cut(s) 297, 816
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 3 cut(s) 40, 894, 1028
BmeT110I CYCGRG 1 cut(s) 1010
BmgT120I GGNCC 3 cut(s) 40, 894, 1028
BmrFI CCNGG 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 520
BplI GAGNNNNNCTC 4 cut(s) 41, 73, 43, 75
BpmI CTGGAG 2 cut(s) 455, 469
Bpu10I CCTNAGC 1 cut(s) 316
Bpu14I TTCGAA 1 cut(s) 491
BpuEI CTTGAG 2 cut(s) 286, 658
BsaJI CCNNGG 3 cut(s) 136, 782, 870
BsaWI WCCGGW 2 cut(s) 891, 1025
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 143
Bse1I ACTGG 2 cut(s) 148, 486
Bse3DI GCAATG 1 cut(s) 649
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 136, 782, 870
BseGI GGATG 4 cut(s) 124, 228, 405, 735
BseLI CCNNNNNNNGG 2 cut(s) 74, 143
BseMI GCAATG 1 cut(s) 649
BseMII CTCAG 2 cut(s) 39, 330
BseNI ACTGG 2 cut(s) 148, 486
BseRI GAGGAG 1 cut(s) 661
BseXI GCAGC 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 147, 732
BsiHKCI CYCGRG 1 cut(s) 1010
BsiSI CCGG 2 cut(s) 892, 1026
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 2 cut(s) 74, 143
BsmAI GTCTC 8 cut(s) 61, 255, 473, 487, 548, 904, 1013, 1027
BsmBI CGTCTC 2 cut(s) 255, 904
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 2 cut(s) 147, 732
BsoBI CYCGRG 1 cut(s) 1010
Bsp119I TTCGAA 1 cut(s) 491
Bsp143I GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
Bsp19I CCATGG 1 cut(s) 870
BspACI CCGC 5 cut(s) 156, 170, 347, 562, 994
BspANI GGCC 2 cut(s) 147, 732
BspCNI CTCAG 2 cut(s) 40, 329
BspFNI CGCG 1 cut(s) 347
BspHI TCATGA 1 cut(s) 417
BspPI GGATC 2 cut(s) 2, 118
BspT104I TTCGAA 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 994
BsrDI GCAATG 1 cut(s) 649
BsrI ACTGG 2 cut(s) 148, 486
BssECI CCNNGG 3 cut(s) 136, 782, 870
BssMI GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
BssSI CACGAG 1 cut(s) 545
BssT1I CCWWGG 2 cut(s) 782, 870
Bst2BI CACGAG 1 cut(s) 545
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 337, 898
Bst6I CTCTTC 1 cut(s) 965
BstBI TTCGAA 1 cut(s) 491
BstDEI CTNAG 4 cut(s) 48, 286, 316, 838
BstDSI CCRYGG 1 cut(s) 870
BstF5I GGATG 4 cut(s) 124, 228, 405, 735
BstFNI CGCG 1 cut(s) 347
BstKTI GATC 8 cut(s) 10, 93, 126, 250, 283, 300, 819, 984
BstMAI GTCTC 8 cut(s) 61, 255, 473, 487, 548, 904, 1013, 1027
BstMBI GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
BstNI CCWGG 1 cut(s) 138
BstNSI RCATGY 2 cut(s) 592, 692
BstSCI CCNGG 1 cut(s) 136
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 520
BstX2I RGATCY 3 cut(s) 123, 297, 816
BstYI RGATCY 3 cut(s) 123, 297, 816
BsuRI GGCC 2 cut(s) 147, 732
BtgI CCRYGG 1 cut(s) 870
BtsCI GGATG 4 cut(s) 124, 228, 405, 735
BtsIMutI CAGTG 1 cut(s) 141
CciI TCATGA 1 cut(s) 417
Cfr13I GGNCC 3 cut(s) 40, 894, 1028
CpoI CGGWCCG 2 cut(s) 894, 1028
CseI GACGC 3 cut(s) 331, 858, 884
Csp6I GTAC 2 cut(s) 361, 684
CspCI CAANNNNNGTGG 2 cut(s) 521, 556
CspI CGGWCCG 2 cut(s) 894, 1028
CviAII CATG 9 cut(s) 245, 418, 511, 524, 589, 662, 689, 871, 1059
CviQI GTAC 2 cut(s) 361, 684
DdeI CTNAG 4 cut(s) 48, 286, 316, 838
DpnI GATC 8 cut(s) 9, 92, 125, 249, 282, 299, 818, 983
DpnII GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
EaeI YGGCCR 2 cut(s) 145, 730
Eam1104I CTCTTC 1 cut(s) 965
EarI CTCTTC 1 cut(s) 965
Eco130I CCWWGG 2 cut(s) 782, 870
Eco32I GATATC 1 cut(s) 393
Eco47I GGWCC 3 cut(s) 40, 894, 1028
Eco88I CYCGRG 1 cut(s) 1010
EcoRII CCWGG 1 cut(s) 136
EcoRV GATATC 1 cut(s) 393
EcoT14I CCWWGG 2 cut(s) 782, 870
ErhI CCWWGG 2 cut(s) 782, 870
Esp3I CGTCTC 2 cut(s) 255, 904
FaeI CATG 9 cut(s) 248, 421, 514, 527, 592, 665, 692, 874, 1062
FaqI GGGAC 1 cut(s) 275
FatI CATG 9 cut(s) 244, 417, 510, 523, 588, 661, 688, 870, 1058
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 4 cut(s) 131, 215, 412, 722
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 4 cut(s) 210, 479, 941, 1019
GluI GCNGC 1 cut(s) 381
GsuI CTGGAG 2 cut(s) 455, 469
HaeIII GGCC 2 cut(s) 147, 732
HapII CCGG 2 cut(s) 892, 1026
HgaI GACGC 3 cut(s) 331, 858, 884
Hin1II CATG 9 cut(s) 248, 421, 514, 527, 592, 665, 692, 874, 1062
HincII GTYRAC 2 cut(s) 328, 373
HindII GTYRAC 2 cut(s) 328, 373
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 6 cut(s) 54, 252, 452, 475, 602, 1015
HpaII CCGG 2 cut(s) 892, 1026
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 5 cut(s) 165, 328, 373, 686, 698
Hpy188I TCNGA 2 cut(s) 740, 1032
Hpy188III TCNNGA 5 cut(s) 418, 472, 946, 1010, 1012
Hpy8I GTNNAC 5 cut(s) 165, 328, 373, 686, 698
Hpy99I CGWCG 3 cut(s) 347, 377, 920
HpyCH4III ACNGT 2 cut(s) 337, 898
HpyCH4IV ACGT 4 cut(s) 161, 363, 700, 908
HpyCH4V TGCA 2 cut(s) 592, 654
HpyF3I CTNAG 4 cut(s) 48, 286, 316, 838
HpySE526I ACGT 4 cut(s) 161, 363, 700, 908
Hsp92II CATG 9 cut(s) 248, 421, 514, 527, 592, 665, 692, 874, 1062
Kzo9I GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
LpnPI CCDG 9 cut(s) 23, 123, 129, 150, 303, 485, 499, 905, 1039
Lsp1109I GCAGC 1 cut(s) 392
MaeI CTAG 4 cut(s) 210, 479, 941, 1019
MaeII ACGT 4 cut(s) 161, 363, 700, 908
MaeIII GTNAC 5 cut(s) 218, 237, 349, 883, 904
MalI GATC 8 cut(s) 9, 92, 125, 249, 282, 299, 818, 983
MbiI CCGCTC 1 cut(s) 994
MboI GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
MboII GAAGA 4 cut(s) 85, 520, 982, 991
MflI RGATCY 3 cut(s) 123, 297, 816
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 4 cut(s) 196, 758, 936, 965
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 6 cut(s) 63, 246, 461, 484, 596, 1024
MnlI CCTC 8 cut(s) 107, 370, 390, 421, 628, 682, 924, 955
Mox20I TGGCCA 1 cut(s) 147
MscI TGGCCA 1 cut(s) 147
MseI TTAA 3 cut(s) 114, 386, 1046
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 147
MspI CCGG 2 cut(s) 892, 1026
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 1059
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 1 cut(s) 347
NcoI CCATGG 1 cut(s) 870
NdeII GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
NlaIII CATG 9 cut(s) 248, 421, 514, 527, 592, 665, 692, 874, 1062
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 2 cut(s) 592, 692
NspV TTCGAA 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 1010
PagI TCATGA 1 cut(s) 417
PciI ACATGT 1 cut(s) 688
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PctI GAATGC 1 cut(s) 1059
PkrI GCNGC 1 cut(s) 382
PleI GAGTC 6 cut(s) 62, 246, 460, 483, 596, 1023
PpsI GAGTC 6 cut(s) 62, 246, 460, 483, 596, 1023
PscI ACATGT 1 cut(s) 688
PsiI TTATAA 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspPI GGNCC 3 cut(s) 40, 894, 1028
PsrI GAACNNNNNNTAC 2 cut(s) 18, 50
PsuI RGATCY 3 cut(s) 123, 297, 816
RsaI GTAC 2 cut(s) 362, 685
RsaNI GTAC 2 cut(s) 361, 684
RseI CAYNNNNRTG 1 cut(s) 509
Rsr2I CGGWCCG 2 cut(s) 894, 1028
RsrII CGGWCCG 2 cut(s) 894, 1028
SalI GTCGAC 1 cut(s) 371
SaqAI TTAA 3 cut(s) 114, 386, 1046
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 8 cut(s) 7, 90, 123, 247, 280, 297, 816, 981
Sau96I GGNCC 3 cut(s) 40, 894, 1028
SchI GAGTC 6 cut(s) 63, 246, 461, 484, 596, 1024
ScrFI CCNGG 1 cut(s) 138
Sfr274I CTCGAG 1 cut(s) 1010
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 3 cut(s) 40, 894, 1028
SlaI CTCGAG 1 cut(s) 1010
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 3 cut(s) 301, 673, 1010
SmoI CTYRAG 3 cut(s) 301, 673, 1010
Sse9I AATT 4 cut(s) 196, 758, 936, 965
SsiI CCGC 5 cut(s) 156, 170, 347, 562, 994
SspI AATATT 1 cut(s) 712
SspMI CTAG 4 cut(s) 210, 479, 941, 1019
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 2 cut(s) 782, 870
TaaI ACNGT 2 cut(s) 337, 898
TaiI ACGT 4 cut(s) 164, 366, 703, 911
TaqI TCGA 8 cut(s) 89, 151, 250, 372, 395, 491, 984, 1011
TasI AATT 4 cut(s) 196, 758, 936, 965
TatI WGTACW 1 cut(s) 683
Tru1I TTAA 3 cut(s) 114, 386, 1046
Tru9I TTAA 3 cut(s) 114, 386, 1046
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 3 cut(s) 17, 406, 1054
TspGWI ACGGA 4 cut(s) 356, 683, 839, 892
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 3 cut(s) 40, 894, 1028
XceI RCATGY 2 cut(s) 592, 692
XhoI CTCGAG 1 cut(s) 1010
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 4 cut(s) 210, 479, 941, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.