AT4G12190

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018729
Physical Location & Seq
Reverse (-)
7278545 .. 7278760
216 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12190.1

Sequence Viewer

Length: 216 bp
ATGGTTCTGCTAGGGAGAATAAAAACAGAGGAATTGAAGAGTTTGAAGATGGAAACAGAGCCGTGTTCCATCTGTCTCGAGAGTCTAGTCTCCGGTCCTAAACCTAGGGACATCACACGCATGACTTGTTCTCATGTCTTCCACAATGGCTGTCTTTTAGAGTGGCTCAAGCGTAAGAACACTTGTCCCCTTTGTCGGACTGAGCTCTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

71

Amino Acids

8.2

Weight (kDa)

8.27

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 19 - 68 6.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 22 - 66 1.9e-14 Ring finger domain
zf-C3HC4 PF00097 22 - 65 1.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 22 - 65 3.2e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 22 - 63 2.9e-08 RING-type zinc-finger
zf-RING_11 PF17123 22 - 52 2.2e-07 RING-like zinc finger
zf-RING_5 PF14634 22 - 67 1.4e-07 zinc-RING finger domain
zf-RING_Vps41 PF23555 22 - 60 1.1e-06 Vps41 C-terminal RING finger domain
zf-rbx1 PF12678 36 - 66 1.8e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05350 AT4G12140 AT4G12150 AT4G12190 AT4G12210 AT4G12220 AT4G18110
rosa_chinensis RchiOBHm_Chr4g0400421 RchiOBHm_Chr4g0400491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 195
AgsI TTSAA 2 cut(s) 37, 46
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 2 cut(s) 80, 94
Ama87I CYCGRG 1 cut(s) 77
AspA2I CCTAGG 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 1 cut(s) 95
AvrII CCTAGG 1 cut(s) 104
BanII GRGCYC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 207
BccI CCATC 2 cut(s) 43, 77
BceAI ACGGC 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 80, 94
BfaI CTAG 3 cut(s) 11, 86, 105
BlnI CCTAGG 1 cut(s) 104
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 104
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 122, 171
BslI CCNNNNNNNGG 1 cut(s) 195
BsmAI GTCTC 2 cut(s) 80, 94
BsmFI GGGAC 2 cut(s) 122, 171
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 104
BssT1I CCWWGG 1 cut(s) 104
Bst6I CTCTTC 1 cut(s) 32
BstDEI CTNAG 1 cut(s) 201
BstMAI GTCTC 2 cut(s) 80, 94
BstV2I GAAGAC 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 95
CviAII CATG 2 cut(s) 121, 134
CviJI RGCY 4 cut(s) 61, 150, 166, 205
CviKI_1 RGCY 4 cut(s) 61, 150, 166, 205
DdeI CTNAG 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 104
Eco24I GRGCYC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 104
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 2 cut(s) 124, 137
FaiI YATR 2 cut(s) 122, 135
FaqI GGGAC 2 cut(s) 122, 171
FatI CATG 2 cut(s) 120, 133
FriOI GRGCYC 1 cut(s) 207
FspBI CTAG 3 cut(s) 11, 86, 105
HapII CCGG 1 cut(s) 93
Hin1II CATG 2 cut(s) 124, 137
HinfI GANTC 1 cut(s) 82
HpaII CCGG 1 cut(s) 93
Hpy188I TCNGA 1 cut(s) 198
Hpy188III TCNNGA 2 cut(s) 77, 79
HpyF3I CTNAG 1 cut(s) 201
Hsp92II CATG 2 cut(s) 124, 137
LpnPI CCDG 1 cut(s) 106
MaeI CTAG 3 cut(s) 11, 86, 105
MboII GAAGA 3 cut(s) 49, 58, 130
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 1 cut(s) 32
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 1 cut(s) 22
MslI CAYNNNNRTG 1 cut(s) 119
MspI CCGG 1 cut(s) 93
NlaIII CATG 2 cut(s) 124, 137
PaeR7I CTCGAG 1 cut(s) 77
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 207
PspPI GGNCC 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 119
SacI GAGCTC 1 cut(s) 207
Sau96I GGNCC 1 cut(s) 95
SchI GAGTC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 207
SetI ASST 2 cut(s) 106, 207
Sfr274I CTCGAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 77, 167
SmoI CTYRAG 2 cut(s) 77, 167
Sse9I AATT 1 cut(s) 32
SspMI CTAG 3 cut(s) 11, 86, 105
SstI GAGCTC 1 cut(s) 207
StyI CCWWGG 1 cut(s) 104
TaqI TCGA 1 cut(s) 78
TasI AATT 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 77
XmaJI CCTAGG 1 cut(s) 104
XspI CTAG 3 cut(s) 11, 86, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.