AT4G12140

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018729
Physical Location & Seq
Reverse (-)
7266546 .. 7267154
609 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12140.1

Sequence Viewer

Length: 609 bp
ATGAATGATCCCATTCCCAAACCCATAGTGAGCGTTCAGGTCCAAGCTGTGAAAGTCTCTACAAACTCGATCTTCCTCATCGTCAATACCATTAAGGATGAGATCCTTATAAACCCTGTCACTGGCCATCGAGCCGAAACGTCCACTCCGCTTTCCTATAAATCACTTCCCATCAATTTCAGTCTCCCTAGTTGTAGTCACCACCACATCCAATCGTTACTCCACGATCAACTCCATAGCGACGACAATTGGTTGTGCGATCACTTAGTCCCAAAGATCTCAACGGCTGCAATCAACGCAGGTTTTATGAACAACGGCGTTGAATTGACCGTTGGCGTCACAGTTACTTACCAGTATGTCCATGTCGAGGAGACATCATCAAAGGAATCGAGGATGGTTCGACTAGGGAGCATGAAAGCAGAGGAATTGAAGAGTTTTAACATGGAAACAGATTCGTGTTCCATCTGTCTACAGAGTCTAGTCTCCAGTTCGAAAACAGGACCCACTCGCATGAGTTGTTCCCATGTCTTCCATAGTAGCTGTCTCGTGGAGTGGCTCAAGAGAAAAAACACTTGCCCGATGTGTCGGACTGTGCTCTACGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

202

Amino Acids

22.52

Weight (kDa)

7.12

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 151 - 196 1.4e-13 Ring finger domain
zf-C3HC4_2 PF13923 153 - 195 2.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 153 - 195 1.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 153 - 197 2.4e-07 zinc-RING finger domain
zf-RING_UBOX PF13445 153 - 193 2e-06 RING-type zinc-finger
zf-RING_11 PF17123 153 - 182 2e-06 RING-like zinc finger
zf-rbx1 PF12678 161 - 196 9.7e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05350 AT4G12140 AT4G12150 AT4G12190 AT4G12210 AT4G12220 AT4G18110
rosa_chinensis RchiOBHm_Chr4g0400421 RchiOBHm_Chr4g0400491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 290
AccI GTMKAC 1 cut(s) 469
AciI CCGC 1 cut(s) 149
AclWI GGATC 2 cut(s) 2, 97
AcoI YGGCCR 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 336
AfiI CCNNNNNNNGG 2 cut(s) 122, 367
AgsI TTSAA 2 cut(s) 323, 430
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AluBI AGCT 2 cut(s) 47, 540
AluI AGCT 2 cut(s) 47, 540
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 5 cut(s) 61, 188, 365, 487, 548
AlwI GGATC 2 cut(s) 2, 97
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 40, 500
AsuHPI GGTGA 1 cut(s) 191
AsuII TTCGAA 1 cut(s) 491
AvaII GGWCC 2 cut(s) 40, 500
BalI TGGCCA 1 cut(s) 126
BauI CACGAG 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 520
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 274
BccI CCATC 4 cut(s) 135, 179, 388, 470
BceAI ACGGC 2 cut(s) 300, 331
BcoDI GTCTC 5 cut(s) 61, 188, 365, 487, 548
BfaI CTAG 3 cut(s) 189, 404, 479
BfmI CTRYAG 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 290
BglII AGATCT 1 cut(s) 276
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme18I GGWCC 2 cut(s) 40, 500
BmgT120I GGNCC 2 cut(s) 40, 500
BmiI GGNNCC 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 520
BpmI CTGGAG 1 cut(s) 469
Bpu14I TTCGAA 1 cut(s) 491
BpuEI CTTGAG 1 cut(s) 542
Bsa29I ATCGAT 1 cut(s) 604
BsaHI GRCGYC 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 367
Bse1I ACTGG 3 cut(s) 127, 352, 486
BseCI ATCGAT 1 cut(s) 604
BseGI GGATG 3 cut(s) 103, 207, 399
BseLI CCNNNNNNNGG 2 cut(s) 122, 367
BseNI ACTGG 3 cut(s) 127, 352, 486
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 1 cut(s) 274
Bsh1285I CGRYCG 1 cut(s) 604
BshFI GGCC 1 cut(s) 126
BshVI ATCGAT 1 cut(s) 604
BsiEI CGRYCG 1 cut(s) 604
BsiHKAI GWGCWC 1 cut(s) 597
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 122, 367
BsmAI GTCTC 5 cut(s) 61, 188, 365, 487, 548
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
BspACI CCGC 1 cut(s) 149
BspANI GGCC 1 cut(s) 126
BspDI ATCGAT 1 cut(s) 604
BspLI GGNNCC 1 cut(s) 502
BspMI ACCTGC 1 cut(s) 290
BspPI GGATC 2 cut(s) 2, 97
BspT104I TTCGAA 1 cut(s) 491
BsrI ACTGG 3 cut(s) 127, 352, 486
BssMI GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 545
Bst2BI CACGAG 1 cut(s) 545
Bst4CI ACNGT 3 cut(s) 331, 343, 592
Bst6I CTCTTC 1 cut(s) 425
BstACI GRCGYC 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 491
BstDEI CTNAG 1 cut(s) 265
BstF5I GGATG 3 cut(s) 103, 207, 399
BstKTI GATC 7 cut(s) 10, 72, 105, 229, 262, 279, 604
BstMAI GTCTC 5 cut(s) 61, 188, 365, 487, 548
BstMBI GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
BstMCI CGRYCG 1 cut(s) 604
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 470
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 520
BstX2I RGATCY 2 cut(s) 102, 276
BstYI RGATCY 2 cut(s) 102, 276
Bsu15I ATCGAT 1 cut(s) 604
BsuRI GGCC 1 cut(s) 126
BsuTUI ATCGAT 1 cut(s) 604
BtsCI GGATG 3 cut(s) 103, 207, 399
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 290
Cfr13I GGNCC 2 cut(s) 40, 500
ClaI ATCGAT 1 cut(s) 604
CseI GACGC 1 cut(s) 325
CviAII CATG 5 cut(s) 362, 412, 442, 511, 524
CviJI RGCY 6 cut(s) 47, 126, 134, 287, 540, 556
CviKI_1 RGCY 6 cut(s) 47, 126, 134, 287, 540, 556
DdeI CTNAG 1 cut(s) 265
DpnI GATC 7 cut(s) 9, 71, 104, 228, 261, 278, 603
DpnII GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Eco47I GGWCC 2 cut(s) 40, 500
EcoO109I RGGNCCY 1 cut(s) 500
FaeI CATG 5 cut(s) 365, 415, 445, 514, 527
FaqI GGGAC 1 cut(s) 254
FatI CATG 5 cut(s) 361, 411, 441, 510, 523
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 3 cut(s) 110, 194, 406
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 3 cut(s) 189, 404, 479
GluI GCNGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 1 cut(s) 126
HgaI GACGC 1 cut(s) 325
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 5 cut(s) 365, 415, 445, 514, 527
HinfI GANTC 3 cut(s) 386, 452, 475
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 2 cut(s) 144, 470
Hpy188I TCNGA 1 cut(s) 588
Hpy188III TCNNGA 1 cut(s) 559
Hpy8I GTNNAC 2 cut(s) 144, 470
Hpy99I CGWCG 1 cut(s) 245
HpyCH4III ACNGT 3 cut(s) 331, 343, 592
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 1 cut(s) 265
HpySE526I ACGT 1 cut(s) 140
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 5 cut(s) 365, 415, 445, 514, 527
Kzo9I GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
LmnI GCTCC 1 cut(s) 408
LpnPI CCDG 7 cut(s) 23, 108, 129, 285, 365, 483, 499
Lsp1109I GCAGC 1 cut(s) 274
MaeI CTAG 3 cut(s) 189, 404, 479
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 5 cut(s) 118, 197, 216, 337, 343
MalI GATC 7 cut(s) 9, 71, 104, 228, 261, 278, 603
MboI GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
MboII GAAGA 3 cut(s) 64, 442, 520
MfeI CAATTG 1 cut(s) 247
MflI RGATCY 2 cut(s) 102, 276
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 4 cut(s) 175, 247, 323, 425
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 1 cut(s) 484
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 4 cut(s) 86, 361, 384, 415
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
MseI TTAA 2 cut(s) 93, 438
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 126
MunI CAATTG 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
NlaIII CATG 5 cut(s) 365, 415, 445, 514, 527
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 3 cut(s) 118, 197, 337
NspV TTCGAA 1 cut(s) 491
PfeI GAWTC 2 cut(s) 386, 452
PkrI GCNGC 1 cut(s) 289
Ple19I CGATCG 1 cut(s) 604
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 500
PsiI TTATAA 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 500
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 2 cut(s) 40, 500
PspPPI RGGWCCY 1 cut(s) 500
PsuI RGATCY 2 cut(s) 102, 276
PvuI CGATCG 1 cut(s) 604
RseI CAYNNNNRTG 1 cut(s) 509
SaqAI TTAA 2 cut(s) 93, 438
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 7 cut(s) 7, 69, 102, 226, 259, 276, 601
Sau96I GGNCC 2 cut(s) 40, 500
SchI GAGTC 1 cut(s) 484
SduI GDGCHC 1 cut(s) 597
SetI ASST 5 cut(s) 42, 49, 143, 304, 542
SfcI CTRYAG 1 cut(s) 470
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 2 cut(s) 40, 500
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 4 cut(s) 175, 247, 323, 425
SsiI CCGC 1 cut(s) 149
SspMI CTAG 3 cut(s) 189, 404, 479
TaaI ACNGT 3 cut(s) 331, 343, 592
TaiI ACGT 1 cut(s) 143
TaqI TCGA 7 cut(s) 68, 130, 366, 389, 400, 491, 604
TasI AATT 4 cut(s) 175, 247, 323, 425
TfiI GAWTC 2 cut(s) 386, 452
Tru1I TTAA 2 cut(s) 93, 438
Tru9I TTAA 2 cut(s) 93, 438
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 3 cut(s) 118, 197, 337
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 3 cut(s) 118, 197, 337
TspDTI ATGAA 3 cut(s) 17, 323, 428
TspRI CASTG 1 cut(s) 127
VpaK11BI GGWCC 2 cut(s) 40, 500
XmiI GTMKAC 1 cut(s) 469
XspI CTAG 3 cut(s) 189, 404, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.