AT4G12210

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018729
Physical Location & Seq
Reverse (-)
7281308 .. 7281919
612 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12210.1

Sequence Viewer

Length: 612 bp
ATGAATTCTCGCAAAGTGGAATTTCGCTTCACCAATCTTCAAAGGAAACTCTCTTCTCCATCACATGCAAACTCAGTCATCGTTGAGATCGATACCAAATCTGAAGAGATCTTTGTAAACCAAACAACCGATCACCAAACCCGCCGGTCCACTCCGGCTACTCTATCTTGTCTCGACACTATCAATCTCAGTAGTTCTAGTCACGACCACATCCGATCACTTCTCCGTACCCGATTCGCTAGCCACCATTGGTTGTGTGATGACCTAGCCTCAGAGATCTCAACGGCGGCTATCGACTTAGGTTTTGGACGAAACGGTTTTACCATCACCGTGGTCGTCACAGTTACTTACCGGACTGTCTCGGTAACGTCAAACAACAAGAAATCTTTGAGGATAGTTCTACTAGGAAGGATAAAAGCAGAGGAATTGAAGAGTTTGAAGATGGAAACAGAGTCTTGTTCAATCTGTCTTGAGAATCTAGTCTCCGGTCCAAAACCTAGCGACCTCACACGCATGACTTGTTCTCATGTCTTCCACAATCCCTGTCTCTTGGAGTGGTTCATGCGTAAAAACACTTGTCCCCTGTGTCGGACGGAGCTCTACGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

203

Amino Acids

23.04

Weight (kDa)

8.83

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 152 - 197 5.1e-13 Ring finger domain
zf-C3HC4 PF00097 153 - 196 9.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 153 - 196 4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 153 - 183 1.8e-06 RING-like zinc finger
zf-RING_5 PF14634 153 - 198 2.6e-06 zinc-RING finger domain
zf-RING_Vps41 PF23555 153 - 191 9.8e-06 Vps41 C-terminal RING finger domain
zf-rbx1 PF12678 167 - 197 8.7e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018729)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05350 AT4G12140 AT4G12150 AT4G12190 AT4G12210 AT4G12220 AT4G18110
rosa_chinensis RchiOBHm_Chr4g0400421 RchiOBHm_Chr4g0400491

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 142, 287
AcsI RAATTY 2 cut(s) 4, 20
AcuI CTGAAG 1 cut(s) 123
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 588
AgsI TTSAA 4 cut(s) 41, 430, 439, 462
AluBI AGCT 1 cut(s) 598
AluI AGCT 1 cut(s) 598
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 4 cut(s) 176, 364, 487, 551
ApoI RAATTY 2 cut(s) 4, 20
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AspS9I GGNCC 2 cut(s) 147, 488
AsuHPI GGTGA 3 cut(s) 22, 125, 319
AsuNHI GCTAGC 1 cut(s) 239
AvaII GGWCC 2 cut(s) 147, 488
BanII GRGCYC 1 cut(s) 600
BbsI GAAGAC 1 cut(s) 523
Bbv12I GWGCWC 1 cut(s) 600
BccI CCATC 3 cut(s) 67, 332, 436
BceAI ACGGC 1 cut(s) 300
BcoDI GTCTC 4 cut(s) 176, 364, 487, 551
BfaI CTAG 6 cut(s) 198, 240, 266, 404, 479, 498
BglII AGATCT 2 cut(s) 108, 276
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme18I GGWCC 2 cut(s) 147, 488
BmgT120I GGNCC 2 cut(s) 147, 488
BmtI GCTAGC 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 491
Bsa29I ATCGAT 2 cut(s) 90, 607
BsaJI CCNNGG 1 cut(s) 330
BsaWI WCCGGW 2 cut(s) 351, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse118I RCCGGY 1 cut(s) 144
BseCI ATCGAT 2 cut(s) 90, 607
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 3 cut(s) 87, 202, 285
Bsh1285I CGRYCG 1 cut(s) 607
BshVI ATCGAT 2 cut(s) 90, 607
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 600
BsiSI CCGG 4 cut(s) 145, 155, 352, 486
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 4 cut(s) 176, 364, 487, 551
BsmFI GGGAC 1 cut(s) 564
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 6 cut(s) 87, 108, 130, 215, 276, 604
BspACI CCGC 2 cut(s) 142, 287
BspCNI CTCAG 3 cut(s) 86, 201, 284
BspDI ATCGAT 2 cut(s) 90, 607
BspOI GCTAGC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 6 cut(s) 87, 108, 130, 215, 276, 604
Bst4CI ACNGT 4 cut(s) 317, 331, 343, 358
Bst6I CTCTTC 3 cut(s) 58, 99, 425
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 4 cut(s) 73, 188, 271, 298
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 210
BstKTI GATC 6 cut(s) 90, 111, 133, 218, 279, 607
BstMAI GTCTC 4 cut(s) 176, 364, 487, 551
BstMBI GATC 6 cut(s) 87, 108, 130, 215, 276, 604
BstMCI CGRYCG 1 cut(s) 607
BstNSI RCATGY 1 cut(s) 68
BstV2I GAAGAC 1 cut(s) 523
BstX2I RGATCY 2 cut(s) 108, 276
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 2 cut(s) 108, 276
Bsu15I ATCGAT 2 cut(s) 90, 607
BsuTUI ATCGAT 2 cut(s) 90, 607
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 147, 488
ClaI ATCGAT 2 cut(s) 90, 607
Csp6I GTAC 1 cut(s) 228
CviAII CATG 4 cut(s) 65, 514, 527, 562
CviJI RGCY 5 cut(s) 158, 243, 269, 290, 598
CviKI_1 RGCY 5 cut(s) 158, 243, 269, 290, 598
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 4 cut(s) 73, 188, 271, 298
DpnI GATC 6 cut(s) 89, 110, 132, 217, 278, 606
DpnII GATC 6 cut(s) 87, 108, 130, 215, 276, 604
Eam1104I CTCTTC 3 cut(s) 58, 99, 425
EarI CTCTTC 3 cut(s) 58, 99, 425
Ecl136II GAGCTC 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 147, 488
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 123
EcoICRI GAGCTC 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 4
EcoT38I GRGCYC 1 cut(s) 600
FaeI CATG 4 cut(s) 68, 517, 530, 565
FaiI YATR 4 cut(s) 66, 515, 528, 563
FaqI GGGAC 1 cut(s) 564
FatI CATG 4 cut(s) 64, 513, 526, 561
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 6 cut(s) 198, 240, 266, 404, 479, 498
GluI GCNGC 1 cut(s) 288
HapII CCGG 4 cut(s) 145, 155, 352, 486
Hin1II CATG 4 cut(s) 68, 517, 530, 565
HinfI GANTC 3 cut(s) 234, 452, 475
HpaII CCGG 4 cut(s) 145, 155, 352, 486
HphI GGTGA 3 cut(s) 22, 125, 319
Hpy166II GTNNAC 2 cut(s) 118, 150
Hpy188I TCNGA 4 cut(s) 103, 215, 274, 591
Hpy188III TCNNGA 3 cut(s) 173, 203, 470
Hpy8I GTNNAC 2 cut(s) 118, 150
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 4 cut(s) 317, 331, 343, 358
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 1 cut(s) 68
HpyF3I CTNAG 4 cut(s) 73, 188, 271, 298
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 4 cut(s) 68, 517, 530, 565
Kzo9I GATC 6 cut(s) 87, 108, 130, 215, 276, 604
LmnI GCTCC 1 cut(s) 595
LpnPI CCDG 6 cut(s) 158, 168, 365, 499, 556, 596
MaeI CTAG 6 cut(s) 198, 240, 266, 404, 479, 498
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 4 cut(s) 200, 337, 343, 364
MalI GATC 6 cut(s) 89, 110, 132, 217, 278, 606
MboI GATC 6 cut(s) 87, 108, 130, 215, 276, 604
MboII GAAGA 6 cut(s) 29, 45, 116, 442, 451, 523
MflI RGATCY 2 cut(s) 108, 276
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 3 cut(s) 4, 20, 425
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 569
MnlI CCTC 4 cut(s) 280, 384, 415, 515
MslI CAYNNNNRTG 2 cut(s) 329, 512
MspI CCGG 4 cut(s) 145, 155, 352, 486
NdeII GATC 6 cut(s) 87, 108, 130, 215, 276, 604
NheI GCTAGC 1 cut(s) 239
NlaIII CATG 4 cut(s) 68, 517, 530, 565
NmuCI GTSAC 2 cut(s) 200, 337
NspI RCATGY 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 2 cut(s) 234, 475
PkrI GCNGC 1 cut(s) 289
Ple19I CGATCG 1 cut(s) 607
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 600
PspPI GGNCC 2 cut(s) 147, 488
PsuI RGATCY 2 cut(s) 108, 276
PvuI CGATCG 1 cut(s) 607
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 2 cut(s) 329, 512
SacI GAGCTC 1 cut(s) 600
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 6 cut(s) 87, 108, 130, 215, 276, 604
Sau96I GGNCC 2 cut(s) 147, 488
SchI GAGTC 1 cut(s) 461
SduI GDGCHC 1 cut(s) 600
SetI ASST 6 cut(s) 267, 304, 371, 499, 507, 600
SinI GGWCC 2 cut(s) 147, 488
SmiMI CAYNNNNRTG 2 cut(s) 329, 512
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 3 cut(s) 4, 20, 425
SsiI CCGC 2 cut(s) 142, 287
SspMI CTAG 6 cut(s) 198, 240, 266, 404, 479, 498
SstI GAGCTC 1 cut(s) 600
TaaI ACNGT 4 cut(s) 317, 331, 343, 358
TaiI ACGT 1 cut(s) 371
TaqI TCGA 4 cut(s) 90, 174, 294, 607
TasI AATT 3 cut(s) 4, 20, 425
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 2 cut(s) 234, 475
TseFI GTSAC 2 cut(s) 200, 337
Tsp45I GTSAC 2 cut(s) 200, 337
TspDTI ATGAA 2 cut(s) 17, 550
TspGWI ACGGA 2 cut(s) 215, 608
VpaK11BI GGWCC 2 cut(s) 147, 488
XapI RAATTY 2 cut(s) 4, 20
XceI RCATGY 1 cut(s) 68
XspI CTAG 6 cut(s) 198, 240, 266, 404, 479, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.