AT4G10150
ERF Family

RING U-box superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013126
Physical Location & Seq
Forward (+)
6327917 .. 6329751
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10150.1

Sequence Viewer

Length: 711 bp
ATGTCTTACAGTGACCCAAACCAAAACCCGATTCCAGAAACTTACGCTCCATCAAACTCAACTGAATCAGAGAAACTGAAACTTTACCAAGCTTTTATCTTCTCTGTTCCAATCTGTTTCACATTCATCGTTCTCTTCGTTCTTTACGTCATTTACCTTCGTCGAAACAGCACCACCAATGTTGATTGGTCTTCTCTTGGCATGCGTGGTGGTACCTTTGTCCCCACCAACAACAATCTCTCAACGGCTGAATTAGGACTGAGCAAAGATATTAGAGAGATGCTTCCTGTTGTCATCTACAAGGAGAGTTTCATAGTCAAAGATTCACAATGTTCAGTGTGTCTTGGGGACTACCAAGCAGAAGAGAAACTCCAACAAATGCCATCATGTGGGCACACTTTTCACATGGAATGTATTGATCTATGGCTCACATCACACACAACATGCCCTCTTTGTCGTCTCTCTCTTATCCCCAAACCGTCCCTAGACCTGTCCCATCAAAGCACAGAGATTGTCTCTTCCATAGAAAACTCTAATGGAGGAGAAGCTTCAACTCAACCAGATTCCCAGTCAGCAACCGAAGCAATAAGTCACACCGATGATGTCGAAGAAGGTAACCGAGATAGTCAAGAGGTCTCTAAGGAGACGGAAGAGAATGACCGAAACAGCGTAGGGACATCTGATGGTTGTTGTACTTGTAGACTTGGTTAA

Protein Analysis

236

Amino Acids

26.16

Weight (kDa)

4.58

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 110 - 153 1.2e-14 Ring finger domain
zf-RING_11 PF17123 110 - 139 1.1e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 110 - 152 4.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 111 - 150 5e-07 RING-type zinc-finger
zf-C3HC4 PF00097 111 - 152 4e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 113 - 153 1.2e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33480 AT4G10150 AT4G10160
fragaria_vesca FvH4_2g00300
malus_domestica MD05G1006000.v1.1
prunus_persica Prupe.8G002900_v2.0.a1
pyrus_communis pycom05g00120
rosa_chinensis RchiOBHm_Chr6g0249581
rosa_laevigata RLG00000015560
rosa_multiflora Rmu_sc0006936.1_g000008 Rmu_sc0013555.1_g000005
rosa_roxburghii Rroxscaffold_7G00217530
rosa_rugosa Rorug05G0493800
rosa_samantha Rh6AG003600 Rh6BG006600 Rh6CG002700 Rh6DG002500
rosa_wichuraiana Rw6G000400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 212
AccB7I CCANNNNNTGG 1 cut(s) 389
AccI GTMKAC 1 cut(s) 700
AfaI GTAC 2 cut(s) 214, 694
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 1 cut(s) 552
AjuI GAANNNNNNNTTGG 2 cut(s) 15, 47
AluBI AGCT 2 cut(s) 92, 548
AluI AGCT 2 cut(s) 92, 548
Alw26I GTCTC 4 cut(s) 464, 520, 638, 640
Asp718I GGTACC 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 396
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanI GGYRCC 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 183
BccI CCATC 4 cut(s) 58, 391, 504, 677
BceAI ACGGC 1 cut(s) 261
BcoDI GTCTC 4 cut(s) 464, 520, 638, 640
BfaI CTAG 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 214
BmrI ACTGGG 1 cut(s) 562
BmsI GCATC 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 562
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BsaI GGTCTC 1 cut(s) 640
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 1 cut(s) 389
Bse1I ACTGG 1 cut(s) 568
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 1 cut(s) 555
BseSI GKGCMC 1 cut(s) 396
BshNI GGYRCC 1 cut(s) 212
BslFI GGGAC 5 cut(s) 206, 362, 466, 478, 688
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 4 cut(s) 464, 520, 638, 640
BsmBI CGTCTC 2 cut(s) 464, 638
BsmFI GGGAC 5 cut(s) 206, 362, 466, 478, 688
Bso31I GGTCTC 1 cut(s) 640
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 1 cut(s) 418
BspCNI CTCAG 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 640
BsrI ACTGG 1 cut(s) 568
BssMI GATC 1 cut(s) 418
Bst4CI ACNGT 2 cut(s) 11, 480
Bst6I CTCTTC 4 cut(s) 140, 357, 523, 645
BstC8I GCNNGC 1 cut(s) 203
BstDEI CTNAG 2 cut(s) 260, 639
BstEII GGTNACC 1 cut(s) 614
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 4 cut(s) 464, 520, 638, 640
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 1 cut(s) 581
BstNSI RCATGY 2 cut(s) 205, 447
BstPI GGTNACC 1 cut(s) 614
BstSLI GKGCMC 1 cut(s) 396
BstV2I GAAGAC 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 16, 342
Cac8I GCNNGC 1 cut(s) 203
Csp6I GTAC 2 cut(s) 213, 693
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 4 cut(s) 202, 387, 406, 444
CviJI RGCY 4 cut(s) 92, 248, 427, 548
CviKI_1 RGCY 4 cut(s) 92, 248, 427, 548
CviQI GTAC 2 cut(s) 213, 693
DdeI CTNAG 2 cut(s) 260, 639
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eam1104I CTCTTC 4 cut(s) 140, 357, 523, 645
EarI CTCTTC 4 cut(s) 140, 357, 523, 645
Eco31I GGTCTC 1 cut(s) 640
Eco91I GGTNACC 1 cut(s) 614
EcoO65I GGTNACC 1 cut(s) 614
Esp3I CGTCTC 2 cut(s) 464, 638
FaeI CATG 4 cut(s) 205, 390, 409, 447
FaiI YATR 7 cut(s) 203, 314, 388, 407, 424, 445, 524
FaqI GGGAC 5 cut(s) 206, 362, 466, 478, 688
FatI CATG 4 cut(s) 201, 386, 405, 443
FblI GTMKAC 1 cut(s) 700
FspBI CTAG 1 cut(s) 485
Hin1II CATG 4 cut(s) 205, 390, 409, 447
HindIII AAGCTT 2 cut(s) 90, 546
HinfI GANTC 4 cut(s) 31, 65, 323, 563
Hpy166II GTNNAC 1 cut(s) 701
Hpy188I TCNGA 2 cut(s) 70, 682
Hpy188III TCNNGA 2 cut(s) 35, 629
Hpy8I GTNNAC 1 cut(s) 701
Hpy99I CGWCG 1 cut(s) 165
HpyAV CCTTC 2 cut(s) 167, 605
HpyCH4III ACNGT 2 cut(s) 11, 480
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 581
HpyF3I CTNAG 2 cut(s) 260, 639
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 4 cut(s) 205, 390, 409, 447
KpnI GGTACC 1 cut(s) 216
Kzo9I GATC 1 cut(s) 418
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 5 cut(s) 48, 300, 503, 573, 581
LweI GCATC 1 cut(s) 270
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 11, 590, 614
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 7 cut(s) 91, 127, 183, 374, 510, 620, 662
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 3 cut(s) 459, 533, 625
MseI TTAA 1 cut(s) 709
MslI CAYNNNNRTG 1 cut(s) 597
MwoI GCNNNNNNNGC 1 cut(s) 581
NdeII GATC 1 cut(s) 418
NlaIII CATG 4 cut(s) 205, 390, 409, 447
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 2 cut(s) 11, 590
NspI RCATGY 2 cut(s) 205, 447
PaeI GCATGC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 144
PfeI GAWTC 4 cut(s) 31, 65, 323, 563
PflMI CCANNNNNTGG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 614
PspN4I GGNNCC 1 cut(s) 214
RsaI GTAC 2 cut(s) 214, 694
RsaNI GTAC 2 cut(s) 213, 693
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 1 cut(s) 709
Sau3AI GATC 1 cut(s) 418
SduI GDGCHC 1 cut(s) 396
SetI ASST 8 cut(s) 94, 150, 159, 218, 492, 550, 616, 636
SfaNI GCATC 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 597
SphI GCATGC 1 cut(s) 205
Sse9I AATT 1 cut(s) 251
SspMI CTAG 1 cut(s) 485
TaaI ACNGT 2 cut(s) 11, 480
TaiI ACGT 1 cut(s) 150
TaqI TCGA 2 cut(s) 163, 606
TaqII GACCGA 1 cut(s) 675
TasI AATT 1 cut(s) 251
TatI WGTACW 1 cut(s) 692
TfiI GAWTC 4 cut(s) 31, 65, 323, 563
Tru1I TTAA 1 cut(s) 709
Tru9I TTAA 1 cut(s) 709
TscAI CASTG 2 cut(s) 16, 342
TseFI GTSAC 2 cut(s) 11, 590
Tsp45I GTSAC 2 cut(s) 11, 590
TspDTI ATGAA 2 cut(s) 115, 301
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 2 cut(s) 16, 342
Van91I CCANNNNNTGG 1 cut(s) 389
XceI RCATGY 2 cut(s) 205, 447
XmiI GTMKAC 1 cut(s) 700
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.