FvH4_2g00300

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013126
Physical Location & Seq
Reverse (-)
403450 .. 406106
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00300.t1

Sequence Viewer

Length: 708 bp
ATGTCTTACAATCATGTCAGATCTCCAGAACCCAACAACAAGTCAAAACCTTCAGGTTCAGGTTCAGGTGCATTCAAGCTCTACCAATTTTGTGTCCCTATCTTGTTCACTTTTATCCTCCTCTTTGTCTTCTACTTGTTCTATTTTCGTCCCCGCCGTCTCAATTGGTCCTCTCTTCGAACGAGGAACGACAGCAATGGCATTGCCGTAGTTGAATCGGGCTTGAAAAAAGAACACAGGGAGATGCTACCCATTGTTGTGTATAACGAAAGCTTCTTTGTCAGGGATACACAATGTGCAGTCTGCCTGGGGGACTATCAAGCAGATGATAGGCTTCAACAAATACCAGCATGTGGTCACACATTTCACATTGATTGCATTGATAGCTGGCTATCCATGCACACTACCTGTCCACTCTGCCGCTTATCCCTCCTTTCCTCTCATAAATCTCAAAATGAATCACCTGATGTACCTCTAGAATTGAGTCATGCGTCTTCTGTTGCAGAGAATGATGTACCATCTTTTTCACCAACACTTCAAGCTTGCGAAGAAACAGAAGCTTCACAGTTCTCTGGGTTAAGAAATGGGGAACCAACAACTTTCCAGAACTGTTCTGAAGAAGATGCAAGAGGTTGTCAATGTGCAGATCCAGCGAGAGAATTAAGGGATGCGAGAATTGGAAATGAAGTGCATGAGCATACTGGTTGA

Protein Analysis

236

Amino Acids

26.45

Weight (kDa)

5.57

Isoelectric Point (pI)

57.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 98 - 141 3.2e-14 Ring finger domain
zf-RING_11 PF17123 98 - 127 5.5e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 98 - 140 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 99 - 140 7.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 99 - 138 5.5e-06 RING-type zinc-finger
zf-rbx1 PF12678 111 - 141 2.9e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33480 AT4G10150 AT4G10160
fragaria_vesca FvH4_2g00300
malus_domestica MD05G1006000.v1.1
prunus_persica Prupe.8G002900_v2.0.a1
pyrus_communis pycom05g00120
rosa_chinensis RchiOBHm_Chr6g0249581
rosa_laevigata RLG00000015560
rosa_multiflora Rmu_sc0006936.1_g000008 Rmu_sc0013555.1_g000005
rosa_roxburghii Rroxscaffold_7G00217530
rosa_rugosa Rorug05G0493800
rosa_samantha Rh6AG003600 Rh6BG006600 Rh6CG002700 Rh6DG002500
rosa_wichuraiana Rw6G000400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AciI CCGC 2 cut(s) 154, 421
AclWI GGATC 1 cut(s) 641
AcuI CTGAAG 2 cut(s) 36, 636
AdeI CACNNNGTG 1 cut(s) 296
AfaI GTAC 2 cut(s) 471, 516
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 5 cut(s) 76, 215, 226, 338, 539
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 5 cut(s) 79, 273, 387, 542, 560
AluI AGCT 5 cut(s) 79, 273, 387, 542, 560
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 453, 519
AsuII TTCGAA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 168
BbsI GAAGAC 2 cut(s) 121, 486
BccI CCATC 1 cut(s) 526
BceAI ACGGC 2 cut(s) 141, 191
BciT130I CCWGG 1 cut(s) 308
BciVI GTATCC 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 1 cut(s) 476
BfuI GTATCC 1 cut(s) 280
BglII AGATCT 1 cut(s) 20
BisI GCNGC 1 cut(s) 421
BlsI GCNGC 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 308
Bme18I GGWCC 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 1 cut(s) 308
BmsI GCATC 3 cut(s) 234, 613, 658
BpiI GAAGAC 2 cut(s) 121, 486
BpmI CTGGAG 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 307
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 706
Bse3DI GCAATG 2 cut(s) 201, 202
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMI GCAATG 2 cut(s) 201, 202
BseNI ACTGG 1 cut(s) 706
BseRI GAGGAG 1 cut(s) 110
BsgI GTGCAG 2 cut(s) 318, 663
BslFI GGGAC 3 cut(s) 80, 135, 326
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 1 cut(s) 164
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 3 cut(s) 80, 135, 326
BsmI GAATGC 1 cut(s) 71
Bsp119I TTCGAA 1 cut(s) 178
Bsp143I GATC 2 cut(s) 20, 646
BspACI CCGC 2 cut(s) 154, 421
BspLI GGNNCC 1 cut(s) 591
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 178
BsrDI GCAATG 2 cut(s) 201, 202
BsrI ACTGG 1 cut(s) 706
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 2 cut(s) 20, 646
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 567, 611
Bst6I CTCTTC 1 cut(s) 180
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 389, 544
BstF5I GGATG 1 cut(s) 673
BstKTI GATC 2 cut(s) 23, 649
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 20, 646
BstMWI GCNNNNNNNGC 3 cut(s) 384, 397, 650
BstNI CCWGG 1 cut(s) 308
BstNSI RCATGY 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 306
BstV2I GAAGAC 2 cut(s) 121, 486
BstX2I RGATCY 2 cut(s) 20, 646
BstYI RGATCY 2 cut(s) 20, 646
BsuI GTATCC 1 cut(s) 280
BtsCI GGATG 1 cut(s) 673
Cac8I GCNNGC 2 cut(s) 389, 544
Cfr13I GGNCC 1 cut(s) 168
CseI GACGC 1 cut(s) 480
Csp6I GTAC 2 cut(s) 470, 515
CviAII CATG 5 cut(s) 14, 351, 397, 488, 692
CviJI RGCY 8 cut(s) 79, 222, 273, 334, 387, 391, 542, 560
CviKI_1 RGCY 8 cut(s) 79, 222, 273, 334, 387, 391, 542, 560
CviQI GTAC 2 cut(s) 470, 515
DpnI GATC 2 cut(s) 22, 648
DpnII GATC 2 cut(s) 20, 646
DraIII CACNNNGTG 1 cut(s) 296
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 36, 636
EcoRII CCWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 5 cut(s) 17, 354, 400, 491, 695
FaiI YATR 8 cut(s) 15, 264, 352, 398, 444, 489, 693, 699
FaqI GGGAC 3 cut(s) 80, 135, 326
FatI CATG 5 cut(s) 13, 350, 396, 487, 691
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 1 cut(s) 421
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 1 cut(s) 421
FspBI CTAG 1 cut(s) 476
GluI GCNGC 1 cut(s) 421
GsuI CTGGAG 1 cut(s) 9
HgaI GACGC 1 cut(s) 480
Hin1II CATG 5 cut(s) 17, 354, 400, 491, 695
HindIII AAGCTT 3 cut(s) 271, 540, 558
HinfI GANTC 3 cut(s) 215, 458, 484
HphI GGTGA 2 cut(s) 453, 519
Hpy166II GTNNAC 2 cut(s) 108, 413
Hpy188I TCNGA 2 cut(s) 20, 616
Hpy188III TCNNGA 3 cut(s) 26, 476, 604
Hpy8I GTNNAC 2 cut(s) 108, 413
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 2 cut(s) 567, 611
HpyCH4V TGCA 8 cut(s) 71, 299, 378, 400, 503, 626, 644, 691
HpyF10VI GCNNNNNNNGC 3 cut(s) 384, 397, 650
Hsp92II CATG 5 cut(s) 17, 354, 400, 491, 695
Kzo9I GATC 2 cut(s) 20, 646
LweI GCATC 3 cut(s) 234, 613, 658
MaeI CTAG 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 356
MalI GATC 2 cut(s) 22, 648
MboI GATC 2 cut(s) 20, 646
MboII GAAGA 6 cut(s) 121, 167, 486, 560, 629, 632
MfeI CAATTG 1 cut(s) 163
MflI RGATCY 2 cut(s) 20, 646
MluCI AATT 5 cut(s) 86, 163, 479, 659, 675
MlyI GAGTC 1 cut(s) 493
MnlI CCTC 8 cut(s) 128, 131, 177, 181, 440, 448, 483, 623
MseI TTAA 2 cut(s) 578, 662
MslI CAYNNNNRTG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 308
MunI CAATTG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 1 cut(s) 308
MwoI GCNNNNNNNGC 3 cut(s) 384, 397, 650
NdeII GATC 2 cut(s) 20, 646
NlaIII CATG 5 cut(s) 17, 354, 400, 491, 695
NlaIV GGNNCC 1 cut(s) 591
NmuCI GTSAC 1 cut(s) 356
NspI RCATGY 1 cut(s) 354
NspV TTCGAA 1 cut(s) 178
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 2 cut(s) 215, 458
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 1 cut(s) 422
PleI GAGTC 1 cut(s) 492
PpsI GAGTC 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 306
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 1 cut(s) 168
PsuI RGATCY 2 cut(s) 20, 646
RsaI GTAC 2 cut(s) 471, 516
RsaNI GTAC 2 cut(s) 470, 515
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 2 cut(s) 578, 662
SatI GCNGC 1 cut(s) 421
Sau3AI GATC 2 cut(s) 20, 646
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 493
ScrFI CCNGG 1 cut(s) 308
SfaNI GCATC 3 cut(s) 234, 613, 658
SfuI TTCGAA 1 cut(s) 178
SinI GGWCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 257
Sse9I AATT 5 cut(s) 86, 163, 479, 659, 675
SsiI CCGC 2 cut(s) 154, 421
SspMI CTAG 1 cut(s) 476
StyD4I CCNGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 567, 611
TaqI TCGA 1 cut(s) 178
TasI AATT 5 cut(s) 86, 163, 479, 659, 675
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 2 cut(s) 215, 458
Tru1I TTAA 2 cut(s) 578, 662
Tru9I TTAA 2 cut(s) 578, 662
TseFI GTSAC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 2 cut(s) 471, 699
Van91I CCANNNNNTGG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 168
XbaI TCTAGA 1 cut(s) 475
XceI RCATGY 1 cut(s) 354
XspI CTAG 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.