RchiOBHm_Chr6g0249581

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013126
Physical Location & Seq
Reverse (-)
5541832 .. 5545088
3257 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22376

Sequence Viewer

Length: 765 bp
ATGTCTTACAATCACGTCTATCCCAGATGTTGCACACTCTCTCCAGAACCCAACAAGCCAAACGACAAACCTTCGGGTTCGGGTGCAGGTGAATTCAAGCTCTACCAATTTTTCGTCTTTTGTGTCCCTATCTTCTTCACTTTCATCCTCCTCTTTGTCTTCTACTTGTTCTATCTCCGCCCTCGACGTGTCAATTGGTCCTCTATTCGAATGAGGACGGCTCCACAAAATGATGACATTAATGACATTGCCACAGTTGAATTGGGCTTGCAAAAAGAACACAGAGAGATGCTACCCATTGTTGTATATAACGAAAGCTTCTCTGTCAGAGATACACAATGTGCAGTATGCCTGGGGGACTATCAATCAGATGATAGGCTTCAACAAATACCAGCCTGTGGCCACACGTTTCACATTGCTTGCATTGATAGCTGGCTATCCATGCACACCACCTGTCCACTCTGCCGCTTATCCCTCCTTTCGTCTCATAAATCCCAAAATGAATCACCTGATGTAACTCTAGAATTGAGTCATGCGTCTTCTGTTGCAGAGAATGATGCACCATCTCTTGCACCGACACCTCAAGCTTGTGAAGAAACAGAAGCTTCACAGTTCTCTGGGTTAAGGAATGGGGAACCAAGAACTGTCCAAAACAGTTCTGAAGAAGATGCAAGAGGTTGTCAATGTGCAGATCACACCAGAGAATTAAGGGATGCCAGAATTGAAAATGAAGTGCATGAGCATAGTGGTGGATCTTCTGCCTGA

Protein Analysis

254

Amino Acids

28.57

Weight (kDa)

5.24

Isoelectric Point (pI)

57.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 113 - 156 6.7e-14 Ring finger domain
zf-RING_11 PF17123 113 - 142 9.7e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 113 - 155 4.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 114 - 155 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 126 - 156 2.2e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33480 AT4G10150 AT4G10160
fragaria_vesca FvH4_2g00300
malus_domestica MD05G1006000.v1.1
prunus_persica Prupe.8G002900_v2.0.a1
pyrus_communis pycom05g00120
rosa_chinensis RchiOBHm_Chr6g0249581
rosa_laevigata RLG00000015560
rosa_multiflora Rmu_sc0006936.1_g000008 Rmu_sc0013555.1_g000005
rosa_roxburghii Rroxscaffold_7G00217530
rosa_rugosa Rorug05G0493800
rosa_samantha Rh6AG003600 Rh6BG006600 Rh6CG002700 Rh6DG002500
rosa_wichuraiana Rw6G000400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 398
AciI CCGC 2 cut(s) 178, 466
AclWI GGATC 1 cut(s) 760
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 681
AdeI CACNNNGTG 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 398
AflIII ACRYGT 2 cut(s) 187, 405
AgsI TTSAA 4 cut(s) 97, 260, 383, 725
AjiI CACGTC 2 cut(s) 16, 188
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 5 cut(s) 100, 318, 432, 587, 605
AluI AGCT 5 cut(s) 100, 318, 432, 587, 605
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 1 cut(s) 760
AoxI GGCC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 92
AseI ATTAAT 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 198
AsuHPI GGTGA 2 cut(s) 101, 498
AsuII TTCGAA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 198
BalI TGGCCA 1 cut(s) 402
BbsI GAAGAC 2 cut(s) 151, 531
BccI CCATC 1 cut(s) 571
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 1 cut(s) 521
BfuAI ACCTGC 1 cut(s) 77
BisI GCNGC 1 cut(s) 466
BlsI GCNGC 1 cut(s) 467
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 198
BmgBI CACGTC 2 cut(s) 16, 188
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 2 cut(s) 222, 636
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 4 cut(s) 279, 547, 658, 703
BpiI GAAGAC 2 cut(s) 151, 531
BplI GAGNNNNNCTC 2 cut(s) 205, 237
BpmI CTGGAG 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse3DI GCAATG 2 cut(s) 246, 414
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 2 cut(s) 144, 718
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMI GCAATG 2 cut(s) 246, 414
BseRI GAGGAG 1 cut(s) 140
BsgI GTGCAG 3 cut(s) 105, 363, 708
BshFI GGCC 1 cut(s) 402
BslFI GGGAC 2 cut(s) 110, 371
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 489
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 2 cut(s) 110, 371
BsnI GGCC 1 cut(s) 402
Bsp119I TTCGAA 1 cut(s) 208
Bsp143I GATC 2 cut(s) 691, 752
BspACI CCGC 2 cut(s) 178, 466
BspANI GGCC 1 cut(s) 402
BspLI GGNNCC 2 cut(s) 222, 636
BspMI ACCTGC 1 cut(s) 77
BspPI GGATC 1 cut(s) 760
BspT104I TTCGAA 1 cut(s) 208
BsrDI GCAATG 2 cut(s) 246, 414
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 2 cut(s) 691, 752
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 4 cut(s) 256, 612, 646, 656
BstBI TTCGAA 1 cut(s) 208
BstC8I GCNNGC 3 cut(s) 269, 421, 434
BstF5I GGATG 2 cut(s) 144, 718
BstKTI GATC 2 cut(s) 694, 755
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 2 cut(s) 691, 752
BstMWI GCNNNNNNNGC 2 cut(s) 429, 442
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 351
BstV2I GAAGAC 2 cut(s) 151, 531
BstX2I RGATCY 1 cut(s) 752
BstYI RGATCY 1 cut(s) 752
BsuRI GGCC 1 cut(s) 402
BtrI CACGTC 2 cut(s) 16, 188
BtsCI GGATG 2 cut(s) 144, 718
BveI ACCTGC 1 cut(s) 77
Cac8I GCNNGC 3 cut(s) 269, 421, 434
Cfr13I GGNCC 1 cut(s) 198
CseI GACGC 1 cut(s) 525
CviAII CATG 3 cut(s) 442, 533, 737
DpnI GATC 2 cut(s) 693, 754
DpnII GATC 2 cut(s) 691, 752
DraIII CACNNNGTG 1 cut(s) 341
EaeI YGGCCR 1 cut(s) 400
EciI GGCGGA 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 198
Eco57I CTGAAG 1 cut(s) 681
EcoRI GAATTC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 351
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 3 cut(s) 445, 536, 740
FaiI YATR 8 cut(s) 307, 309, 349, 443, 489, 534, 738, 744
FaqI GGGAC 2 cut(s) 110, 371
FatI CATG 3 cut(s) 441, 532, 736
Fnu4HI GCNGC 1 cut(s) 466
FokI GGATG 2 cut(s) 131, 725
Fsp4HI GCNGC 1 cut(s) 466
FspBI CTAG 1 cut(s) 521
GluI GCNGC 1 cut(s) 466
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 402
HgaI GACGC 1 cut(s) 525
Hin1II CATG 3 cut(s) 445, 536, 740
HindIII AAGCTT 3 cut(s) 316, 585, 603
HinfI GANTC 2 cut(s) 503, 529
HphI GGTGA 2 cut(s) 101, 498
Hpy166II GTNNAC 1 cut(s) 458
Hpy188I TCNGA 3 cut(s) 329, 370, 661
Hpy188III TCNNGA 2 cut(s) 44, 521
Hpy8I GTNNAC 1 cut(s) 458
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 81
HpyCH4III ACNGT 4 cut(s) 256, 612, 646, 656
HpyCH4IV ACGT 3 cut(s) 15, 187, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 429, 442
HpySE526I ACGT 3 cut(s) 15, 187, 407
Hsp92II CATG 3 cut(s) 445, 536, 740
Kzo9I GATC 2 cut(s) 691, 752
LmnI GCTCC 1 cut(s) 226
LweI GCATC 4 cut(s) 279, 547, 658, 703
MaeI CTAG 1 cut(s) 521
MaeII ACGT 3 cut(s) 15, 187, 407
MaeIII GTNAC 1 cut(s) 514
MalI GATC 2 cut(s) 693, 754
MboI GATC 2 cut(s) 691, 752
MboII GAAGA 8 cut(s) 124, 127, 151, 531, 605, 674, 677, 747
MfeI CAATTG 1 cut(s) 193
MflI RGATCY 1 cut(s) 752
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 7 cut(s) 92, 107, 193, 260, 524, 704, 720
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 1 cut(s) 538
MnlI CCTC 8 cut(s) 158, 161, 192, 207, 211, 485, 591, 668
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 3 cut(s) 240, 623, 707
MslI CAYNNNNRTG 1 cut(s) 747
Msp20I TGGCCA 1 cut(s) 402
MspR9I CCNGG 1 cut(s) 353
MunI CAATTG 1 cut(s) 193
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 429, 442
NdeII GATC 2 cut(s) 691, 752
NlaIII CATG 3 cut(s) 445, 536, 740
NlaIV GGNNCC 2 cut(s) 222, 636
NspV TTCGAA 1 cut(s) 208
PaqCI CACCTGC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 503
PflMI CCANNNNNTGG 1 cut(s) 398
PkrI GCNGC 1 cut(s) 467
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PshBI ATTAAT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 222, 636
PspPI GGNCC 1 cut(s) 198
PsuI RGATCY 1 cut(s) 752
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 3 cut(s) 240, 623, 707
SatI GCNGC 1 cut(s) 466
Sau3AI GATC 2 cut(s) 691, 752
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 1 cut(s) 538
ScrFI CCNGG 1 cut(s) 353
SfaNI GCATC 4 cut(s) 279, 547, 658, 703
SfuI TTCGAA 1 cut(s) 208
SinI GGWCC 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 7 cut(s) 92, 107, 193, 260, 524, 704, 720
SsiI CCGC 2 cut(s) 178, 466
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 1 cut(s) 351
TaaI ACNGT 4 cut(s) 256, 612, 646, 656
TaiI ACGT 3 cut(s) 18, 190, 410
TaqI TCGA 2 cut(s) 184, 208
TasI AATT 7 cut(s) 92, 107, 193, 260, 524, 704, 720
TauI GCSGC 1 cut(s) 468
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 3 cut(s) 240, 623, 707
Tru9I TTAA 3 cut(s) 240, 623, 707
TspDTI ATGAA 3 cut(s) 133, 516, 744
Van91I CCANNNNNTGG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 198
VspI ATTAAT 1 cut(s) 240
XapI RAATTY 1 cut(s) 92
XbaI TCTAGA 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.