AT4G13400

non-haem dioxygenase in morphine synthesis N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011025
Physical Location & Seq
Reverse (-)
7787622 .. 7791292
3671 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13400.2

Sequence Viewer

Length: 1074 bp
ATGTCTCTCTCGTCGGACTCCACCGTTCCTACTGTTTCCACGGTCACCATATCCTACTCAGAGCTCAAGGAGAGCAACATAGATTTGTCTGCAAGGATTGAAGAAGGATTTGGACCTAATGGACTTGGAATCCTCTCTGTTAAAGATGTTCCTGGATATTCTGCTTTAAGACAGAATCTACTTCAGCTTGCACCTAGGTTAGCTGGTCTTCCTGAAGAGGTGAAGAGGGAGCTTGAAGATCCTCATAGTAGGTATAATTTTGGTTGGAGTCATGGCAAGGAGAAACTTGAATCCGGGAAGCTTGACATGCTGAAGGGTTCTTACTATGCTAATCCTCTACAAGATGTACCTACGAGTAATTCATATGAAATACAGCGGTATCCATCGTATTGTGGATCAAATATATGGCCAAGGAATTCTTTACCGGAACTTGAAGGAGCTTTCAAAGCTCTTGGTAAACTGATGTTCGAAGTTGGGCTAATGGTAGCTTACCACTGTGATCAATATGTGTCAAAGGGTATAAAACAACATGAAAAGCAAAATCTTGAAAAGATACTACTTGGCTCTCGGTGTCACAAAGGACGGCTGCTTTATTATTTCCCTGCACAAGAGAGCTCTACGCATGACAACGATTCCATCTCATCTTGGTGTGGATGGCATACAGACCATGGTTCACTCACAGGTCTTACTCGAGCAATATTTTCTAGAGATTCAGTTGAAGTACCTTGCCCAGATCCTGCTTCTGGCTTATACATACAAACACGATCAGGTCAGATCGTTAAGGTTGTGTATGGGGAAGATGAAATAGCATACCAAATAGGCGAGACAACATCAATTCTGTCTAGTGGTTATCTTTGTGCTACACCTCATTGTGTTCGGGCGCCACAAGGAGAAGAGGCGCGTGGTCTAGAGCGATCGACATTCGCGTTGTTCATGCAACCTGACTGGGACCAGAAGCTTACATTCCCAAAAGAAGTTACAATACATGAAGAGTTATGTCTCTCTGATGAAGTCTTGACGTTTGGAGAATATACAGAGAAACTGCTCAACAAATACTATGATACAAAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

357

Amino Acids

39.99

Weight (kDa)

5.37

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13400 AT4G13400
fragaria_vesca FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370
malus_domestica MD02G1062200.v1.1
prunus_persica Prupe.7G227200_v2.0.a1
pyrus_communis pycom02g04380
rosa_chinensis RchiOBHm_Chr2g0091051
rosa_laevigata RLG00000016171
rosa_multiflora Rmu_sc0005171.1_g000013
rosa_roxburghii Rroxscaffold_2G00150650
rosa_rugosa Rorug02G0011800 Rorug02G0011900
rosa_samantha Rh2AG058200 Rh2CG058800
rosa_wichuraiana Rw2G005100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 880
AccII CGCG 2 cut(s) 901, 926
AciI CCGC 1 cut(s) 376
AclWI GGATC 3 cut(s) 233, 403, 728
AcoI YGGCCR 1 cut(s) 407
AcsI RAATTY 1 cut(s) 415
AcuI CTGAAG 3 cut(s) 167, 234, 332
AcyI GRCGYC 1 cut(s) 881
AfaI GTAC 2 cut(s) 348, 723
AfiI CCNNNNNNNGG 1 cut(s) 743
AgsI TTSAA 7 cut(s) 101, 236, 290, 434, 445, 548, 719
AjnI CCWGG 1 cut(s) 151
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
Alw21I GWGCWC 2 cut(s) 66, 617
Alw26I GTCTC 3 cut(s) 9, 818, 1004
AlwI GGATC 3 cut(s) 233, 403, 728
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 690
AoxI GGCC 1 cut(s) 407
ApeKI GCWGC 1 cut(s) 586
ApoI RAATTY 1 cut(s) 415
AspA2I CCTAGG 1 cut(s) 194
AspLEI GCGC 2 cut(s) 883, 901
AspS9I GGNCC 2 cut(s) 113, 949
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 37, 232
AsuII TTCGAA 1 cut(s) 468
AvaI CYCGRG 1 cut(s) 690
AvaII GGWCC 2 cut(s) 113, 949
AvrII CCTAGG 1 cut(s) 194
BaeI ACNNNNGTAYC 2 cut(s) 362, 395
BalI TGGCCA 1 cut(s) 409
BanI GGYRCC 1 cut(s) 880
BanII GRGCYC 2 cut(s) 66, 617
BarI GAAGNNNNNNTAC 4 cut(s) 305, 337, 966, 998
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 2 cut(s) 66, 617
BbvI GCAGC 1 cut(s) 573
BccI CCATC 3 cut(s) 391, 644, 648
BceAI ACGGC 1 cut(s) 599
BciT130I CCWGG 1 cut(s) 153
BciVI GTATCC 1 cut(s) 390
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 1 cut(s) 295
BcoDI GTCTC 3 cut(s) 9, 818, 1004
BfaI CTAG 4 cut(s) 195, 705, 843, 908
BfoI RGCGCY 1 cut(s) 884
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 1 cut(s) 587
BlnI CCTAGG 1 cut(s) 194
BlsI GCNGC 1 cut(s) 588
Bme1390I CCNGG 2 cut(s) 153, 295
Bme18I GGWCC 2 cut(s) 113, 949
BmeT110I CYCGRG 1 cut(s) 690
BmgT120I GGNCC 2 cut(s) 113, 949
BmiI GGNNCC 2 cut(s) 882, 950
BmrFI CCNGG 2 cut(s) 153, 295
BmrI ACTGGG 1 cut(s) 955
BmuI ACTGGG 1 cut(s) 955
BpiI GAAGAC 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 881
BsaJI CCNNGG 4 cut(s) 39, 194, 410, 667
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 1 cut(s) 743
Bse1I ACTGG 1 cut(s) 950
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 4 cut(s) 39, 194, 410, 667
BseGI GGATG 1 cut(s) 659
BseLI CCNNNNNNNGG 1 cut(s) 743
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 1 cut(s) 950
BseXI GCAGC 1 cut(s) 573
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 2 cut(s) 901, 926
Bsh1285I CGRYCG 1 cut(s) 917
BshFI GGCC 1 cut(s) 409
BshNI GGYRCC 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 917
BsiHKAI GWGCWC 2 cut(s) 66, 617
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 2 cut(s) 294, 425
BslFI GGGAC 1 cut(s) 962
BslI CCNNNNNNNGG 1 cut(s) 743
BsmAI GTCTC 3 cut(s) 9, 818, 1004
BsmFI GGGAC 1 cut(s) 962
BsnI GGCC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 690
Bsp119I TTCGAA 1 cut(s) 468
Bsp1286I GDGCHC 2 cut(s) 66, 617
Bsp143I GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 1 cut(s) 376
BspANI GGCC 1 cut(s) 409
BspCNI CTCAG 1 cut(s) 71
BspFNI CGCG 2 cut(s) 901, 926
BspLI GGNNCC 2 cut(s) 882, 950
BspPI GGATC 3 cut(s) 233, 403, 728
BspT104I TTCGAA 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 880
BsrI ACTGG 1 cut(s) 950
BssECI CCNNGG 4 cut(s) 39, 194, 410, 667
BssMI GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
BssNI GRCGYC 1 cut(s) 881
BssT1I CCWWGG 3 cut(s) 194, 410, 667
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 4 cut(s) 25, 34, 43, 497
Bst6I CTCTTC 4 cut(s) 210, 218, 888, 984
BstACI GRCGYC 1 cut(s) 881
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 2 cut(s) 58, 1071
BstDSI CCRYGG 2 cut(s) 39, 667
BstEII GGTNACC 1 cut(s) 43
BstF5I GGATG 1 cut(s) 659
BstFNI CGCG 2 cut(s) 901, 926
BstH2I RGCGCY 1 cut(s) 884
BstHHI GCGC 2 cut(s) 883, 901
BstKTI GATC 7 cut(s) 241, 398, 502, 736, 767, 777, 917
BstMAI GTCTC 3 cut(s) 9, 818, 1004
BstMBI GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
BstMCI CGRYCG 1 cut(s) 917
BstMWI GCNNNNNNNGC 2 cut(s) 307, 446
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 1 cut(s) 310
BstPI GGTNACC 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 151, 293
BstUI CGCG 2 cut(s) 901, 926
BstV1I GCAGC 1 cut(s) 573
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 2 cut(s) 238, 733
BstYI RGATCY 2 cut(s) 238, 733
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 409
BtgI CCRYGG 2 cut(s) 39, 667
BtsCI GGATG 1 cut(s) 659
BtsIMutI CAGTG 1 cut(s) 493
Cac8I GCNNGC 1 cut(s) 189
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 2 cut(s) 883, 901
Cfr13I GGNCC 2 cut(s) 113, 949
Csp6I GTAC 2 cut(s) 347, 722
CviAII CATG 7 cut(s) 272, 307, 530, 623, 668, 934, 986
CviQI GTAC 2 cut(s) 347, 722
DdeI CTNAG 2 cut(s) 58, 1071
DinI GGCGCC 1 cut(s) 882
DpnI GATC 7 cut(s) 240, 397, 501, 735, 766, 776, 916
DpnII GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
EaeI YGGCCR 1 cut(s) 407
Eam1104I CTCTTC 4 cut(s) 210, 218, 888, 984
EarI CTCTTC 4 cut(s) 210, 218, 888, 984
Ecl136II GAGCTC 2 cut(s) 64, 615
Eco130I CCWWGG 3 cut(s) 194, 410, 667
Eco24I GRGCYC 2 cut(s) 66, 617
Eco47I GGWCC 2 cut(s) 113, 949
Eco53kI GAGCTC 2 cut(s) 64, 615
Eco57I CTGAAG 3 cut(s) 167, 234, 332
Eco88I CYCGRG 1 cut(s) 690
Eco91I GGTNACC 1 cut(s) 43
EcoICRI GAGCTC 2 cut(s) 64, 615
EcoO65I GGTNACC 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 3 cut(s) 194, 410, 667
EcoT38I GRGCYC 2 cut(s) 66, 617
EgeI GGCGCC 1 cut(s) 882
EheI GGCGCC 1 cut(s) 882
ErhI CCWWGG 3 cut(s) 194, 410, 667
FaeI CATG 7 cut(s) 275, 310, 533, 626, 671, 937, 989
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FaqI GGGAC 1 cut(s) 962
FatI CATG 7 cut(s) 271, 306, 529, 622, 667, 933, 985
FauNDI CATATG 1 cut(s) 364
FbaI TGATCA 1 cut(s) 499
Fnu4HI GCNGC 1 cut(s) 587
FokI GGATG 1 cut(s) 666
FriOI GRGCYC 2 cut(s) 66, 617
Fsp4HI GCNGC 1 cut(s) 587
FspBI CTAG 4 cut(s) 195, 705, 843, 908
GlaI GCGC 2 cut(s) 882, 900
GluI GCNGC 1 cut(s) 587
HaeII RGCGCY 1 cut(s) 884
HaeIII GGCC 1 cut(s) 409
HapII CCGG 2 cut(s) 294, 425
HhaI GCGC 2 cut(s) 883, 901
Hin1I GRCGYC 1 cut(s) 881
Hin1II CATG 7 cut(s) 275, 310, 533, 626, 671, 937, 989
Hin6I GCGC 2 cut(s) 881, 899
HinP1I GCGC 2 cut(s) 881, 899
HindIII AAGCTT 2 cut(s) 299, 956
HinfI GANTC 7 cut(s) 17, 129, 175, 268, 290, 632, 710
HpaII CCGG 2 cut(s) 294, 425
HphI GGTGA 2 cut(s) 37, 232
Hpy166II GTNNAC 2 cut(s) 458, 674
Hpy188I TCNGA 4 cut(s) 16, 61, 774, 1006
Hpy188III TCNNGA 5 cut(s) 212, 545, 705, 908, 1015
Hpy8I GTNNAC 2 cut(s) 458, 674
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 3 cut(s) 98, 307, 428
HpyCH4III ACNGT 4 cut(s) 25, 34, 43, 497
HpyCH4IV ACGT 1 cut(s) 1019
HpyCH4V TGCA 4 cut(s) 92, 191, 605, 937
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 446
HpyF3I CTNAG 2 cut(s) 58, 1071
HpySE526I ACGT 1 cut(s) 1019
Hsp92I GRCGYC 1 cut(s) 881
Hsp92II CATG 7 cut(s) 275, 310, 533, 626, 671, 937, 989
HspAI GCGC 2 cut(s) 881, 899
KasI GGCGCC 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
LmnI GCTCC 2 cut(s) 229, 437
Lsp1109I GCAGC 1 cut(s) 573
MaeI CTAG 4 cut(s) 195, 705, 843, 908
MaeII ACGT 1 cut(s) 1019
MaeIII GTNAC 3 cut(s) 43, 572, 976
MalI GATC 7 cut(s) 240, 397, 501, 735, 766, 776, 916
MboI GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
MboII GAAGA 8 cut(s) 113, 200, 227, 235, 248, 809, 905, 1001
MflI RGATCY 2 cut(s) 238, 733
MhlI GDGCHC 2 cut(s) 66, 617
MlsI TGGCCA 1 cut(s) 409
MluCI AATT 4 cut(s) 256, 358, 415, 834
MluNI TGGCCA 1 cut(s) 409
Mly113I GGCGCC 1 cut(s) 881
MlyI GAGTC 2 cut(s) 11, 277
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 7 cut(s) 143, 211, 219, 252, 345, 876, 889
Mox20I TGGCCA 1 cut(s) 409
MscI TGGCCA 1 cut(s) 409
MseI TTAA 3 cut(s) 141, 167, 780
MslI CAYNNNNRTG 1 cut(s) 646
Msp20I TGGCCA 1 cut(s) 409
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 2 cut(s) 294, 425
MspR9I CCNGG 2 cut(s) 153, 295
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 2 cut(s) 901, 926
MwoI GCNNNNNNNGC 2 cut(s) 307, 446
NarI GGCGCC 1 cut(s) 881
NciI CCSGG 1 cut(s) 295
NcoI CCATGG 1 cut(s) 667
NdeI CATATG 1 cut(s) 364
NdeII GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
NlaIII CATG 7 cut(s) 275, 310, 533, 626, 671, 937, 989
NlaIV GGNNCC 2 cut(s) 882, 950
NmuCI GTSAC 2 cut(s) 43, 572
NspI RCATGY 1 cut(s) 310
NspV TTCGAA 1 cut(s) 468
PaeR7I CTCGAG 1 cut(s) 690
PcsI WCGNNNNNNNCGW 1 cut(s) 923
PfeI GAWTC 5 cut(s) 129, 175, 290, 632, 710
PfoI TCCNGGA 2 cut(s) 151, 293
PkrI GCNGC 1 cut(s) 588
Ple19I CGATCG 1 cut(s) 917
PleI GAGTC 2 cut(s) 11, 276
PluTI GGCGCC 1 cut(s) 884
PpsI GAGTC 2 cut(s) 11, 276
Psp124BI GAGCTC 2 cut(s) 66, 617
Psp6I CCWGG 1 cut(s) 151
PspEI GGTNACC 1 cut(s) 43
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 2 cut(s) 882, 950
PspPI GGNCC 2 cut(s) 113, 949
PspXI VCTCGAGB 1 cut(s) 690
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 2 cut(s) 238, 733
PvuI CGATCG 1 cut(s) 917
RsaI GTAC 2 cut(s) 348, 723
RsaNI GTAC 2 cut(s) 347, 722
RseI CAYNNNNRTG 1 cut(s) 646
SacI GAGCTC 2 cut(s) 66, 617
SaqAI TTAA 3 cut(s) 141, 167, 780
SatI GCNGC 1 cut(s) 587
Sau3AI GATC 7 cut(s) 238, 395, 499, 733, 764, 774, 914
Sau96I GGNCC 2 cut(s) 113, 949
SchI GAGTC 2 cut(s) 11, 277
ScrFI CCNGG 2 cut(s) 153, 295
SduI GDGCHC 2 cut(s) 66, 617
SfoI GGCGCC 1 cut(s) 882
Sfr274I CTCGAG 1 cut(s) 690
SfuI TTCGAA 1 cut(s) 468
SinI GGWCC 2 cut(s) 113, 949
SlaI CTCGAG 1 cut(s) 690
SmiMI CAYNNNNRTG 1 cut(s) 646
SmlI CTYRAG 2 cut(s) 65, 690
SmoI CTYRAG 2 cut(s) 65, 690
Sse9I AATT 4 cut(s) 256, 358, 415, 834
SsiI CCGC 1 cut(s) 376
SspDI GGCGCC 1 cut(s) 880
SspI AATATT 1 cut(s) 699
SspMI CTAG 4 cut(s) 195, 705, 843, 908
SstI GAGCTC 2 cut(s) 66, 617
StyD4I CCNGG 2 cut(s) 151, 293
StyI CCWWGG 3 cut(s) 194, 410, 667
TaaI ACNGT 4 cut(s) 25, 34, 43, 497
TaiI ACGT 1 cut(s) 1022
TaqI TCGA 3 cut(s) 468, 691, 917
TasI AATT 4 cut(s) 256, 358, 415, 834
TfiI GAWTC 5 cut(s) 129, 175, 290, 632, 710
Tru1I TTAA 3 cut(s) 141, 167, 780
Tru9I TTAA 3 cut(s) 141, 167, 780
TscAI CASTG 1 cut(s) 500
TseFI GTSAC 2 cut(s) 43, 572
TseI GCWGC 1 cut(s) 586
Tsp45I GTSAC 2 cut(s) 43, 572
TspDTI ATGAA 7 cut(s) 351, 381, 546, 816, 922, 1002, 1023
TspRI CASTG 1 cut(s) 500
VpaK11BI GGWCC 2 cut(s) 113, 949
XapI RAATTY 1 cut(s) 415
XbaI TCTAGA 2 cut(s) 704, 907
XceI RCATGY 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 690
XmaJI CCTAGG 1 cut(s) 194
XspI CTAG 4 cut(s) 195, 705, 843, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.