MD02G1062200.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011025
Physical Location & Seq
Forward (+)
4998576 .. 5003034
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1062200.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGCCTCCGTCCTCCGACCCCGCCGTCCCCACCGTCACACCAGTCACCATATCCTACTCCGAGCTCCAAGACAGGGACAAAGATTTGTCAGTGAAGATTGAAGAAGGATTTGGACCAAATGGGTTGGGAATCCTATCAATCACTCAAGTTCCGGGATATTCTTCCTTGCGCGGGAATCTTCTAGGCTTGTCACCTAGATTAGCCAATCTGCCCGAAGAGGTGAAGAAGGAACTTGAAGATCCTCATAGTAGGTACAATTTTGGATGGAGTCATGGAAAAGAGAAGCTGGAATCTGGAAAGCCTGATACGTTAAAAGGCTCGTTTTATGCAAATCCAATATTGGATAGCCCTACAACAGATGAATCTCTAATTCAAAGGTATCCATCATACTGTGGTTCAAATATATGGCCCAATAGTGAATTGCCAGAACTAGAAGTCGCCTTCAAAGCTCTTGGAAAGCTGATCCTTGGAGTTGGGCTTATGGTTGCATATCACTGTGACAGATACGTGTCAAAAGCAATCAAAATGCGTGAGGATGAAGGCCTTGAACAAATACTGTTTCGATCTCGCTGTCATAAAGGGCGTCTACTTTATTATTTTCCAACAAAAGAAAGTAATCCTACTGAAGATAATAGAGACATGTCTTCTTGGTGTGGATGGCATACAGACCATGGTTCACTGACAGGTCTGACCTGTGCCATGTTTACGCGAGATGCTGCTGAAATACCTTGCCCTGACAGTGGTGCTGGCCTTTATATTAGGACACGAACTGATCAGATTGTCAAAGTCGTTTTTGGAGAAGATGCAATAGCATACCAAATTGGCGAAACCACTGAAGTTCTGTCAAGAGGCTATCTTTGTGCAACGCCTCATTGTGTCCGGGCACCCAAGGGAGCGGAGGCTTCTGGCCTTGATCGTTCAACATTTGCCTTATTCATGCAACCAGACTGGGACGAAAAGCTTAATTTTCCGGAGGAGGTTCATATCCATAAAGAGTTAATTCCACCAAACGGTGCTCTTACCTTCGGAGAGTACACAGAGAAGCTCCTTGACAAATACTACCACCTGAAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

359

Amino Acids

39.97

Weight (kDa)

5.38

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13400 AT4G13400
fragaria_vesca FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370
malus_domestica MD02G1062200.v1.1
prunus_persica Prupe.7G227200_v2.0.a1
pyrus_communis pycom02g04380
rosa_chinensis RchiOBHm_Chr2g0091051
rosa_laevigata RLG00000016171
rosa_multiflora Rmu_sc0005171.1_g000013
rosa_roxburghii Rroxscaffold_2G00150650
rosa_rugosa Rorug02G0011800 Rorug02G0011900
rosa_samantha Rh2AG058200 Rh2CG058800
rosa_wichuraiana Rw2G005100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 883
AccBSI CCGCTC 1 cut(s) 896
AccI GTMKAC 1 cut(s) 586
AccII CGCG 2 cut(s) 171, 709
AccIII TCCGGA 1 cut(s) 970
AciI CCGC 3 cut(s) 21, 171, 896
AclWI GGATC 2 cut(s) 233, 457
AcuI CTGAAG 2 cut(s) 645, 855
AcyI GRCGYC 1 cut(s) 583
AfaI GTAC 2 cut(s) 254, 1034
AfiI CCNNNNNNNGG 4 cut(s) 73, 171, 740, 1010
AflIII ACRYGT 2 cut(s) 507, 639
AgsI TTSAA 7 cut(s) 101, 236, 374, 399, 445, 548, 921
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
AluBI AGCT 6 cut(s) 64, 286, 449, 460, 961, 1045
AluI AGCT 6 cut(s) 64, 286, 449, 460, 961, 1045
Alw21I GWGCWC 2 cut(s) 66, 1018
Alw26I GTCTC 1 cut(s) 630
AlwI GGATC 2 cut(s) 233, 457
Aor13HI TCCGGA 1 cut(s) 970
AoxI GGCC 4 cut(s) 407, 541, 748, 907
ApeKI GCWGC 1 cut(s) 716
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 2 cut(s) 113, 408
AsuC2I CCSGG 2 cut(s) 153, 881
AsuHPI GGTGA 3 cut(s) 37, 183, 232
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 886
BanI GGYRCC 1 cut(s) 883
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 2 cut(s) 66, 1018
BbvI GCAGC 1 cut(s) 703
BccI CCATC 3 cut(s) 258, 391, 651
BceAI ACGGC 1 cut(s) 8
BciVI GTATCC 1 cut(s) 390
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 2 cut(s) 153, 881
BcoDI GTCTC 1 cut(s) 630
BfaI CTAG 3 cut(s) 182, 195, 431
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 1 cut(s) 717
BlsI GCNGC 1 cut(s) 718
Bme1390I CCNGG 2 cut(s) 153, 881
Bme18I GGWCC 1 cut(s) 113
BmgT120I GGNCC 2 cut(s) 113, 408
BmiI GGNNCC 1 cut(s) 885
BmrFI CCNGG 2 cut(s) 153, 881
BmrI ACTGGG 1 cut(s) 958
BmsI GCATC 2 cut(s) 703, 793
BmuI ACTGGG 1 cut(s) 958
BpiI GAAGAC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 129
BpuMI CCSGG 2 cut(s) 153, 881
BsaAI YACGTR 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 583
BsaJI CCNNGG 3 cut(s) 466, 670, 888
BsaWI WCCGGW 1 cut(s) 970
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 171, 740, 1010
Bse1I ACTGG 2 cut(s) 41, 953
BseAI TCCGGA 1 cut(s) 970
BseDI CCNNGG 3 cut(s) 466, 670, 888
BseGI GGATG 3 cut(s) 269, 541, 662
BseLI CCNNNNNNNGG 4 cut(s) 73, 171, 740, 1010
BseNI ACTGG 2 cut(s) 41, 953
BseRI GAGGAG 1 cut(s) 989
BseSI GKGCMC 1 cut(s) 886
BseXI GCAGC 1 cut(s) 703
Bsh1236I CGCG 2 cut(s) 171, 709
BshFI GGCC 4 cut(s) 409, 543, 750, 909
BshNI GGYRCC 1 cut(s) 883
BsiHKAI GWGCWC 2 cut(s) 66, 1018
BsiSI CCGG 3 cut(s) 152, 880, 971
BslFI GGGAC 3 cut(s) 11, 89, 965
BslI CCNNNNNNNGG 4 cut(s) 73, 171, 740, 1010
BsmAI GTCTC 1 cut(s) 630
BsmFI GGGAC 3 cut(s) 11, 89, 965
BsnI GGCC 4 cut(s) 409, 543, 750, 909
Bsp1286I GDGCHC 3 cut(s) 66, 886, 1018
Bsp13I TCCGGA 1 cut(s) 970
Bsp143I GATC 5 cut(s) 238, 462, 563, 772, 913
Bsp19I CCATGG 1 cut(s) 670
BspACI CCGC 3 cut(s) 21, 171, 896
BspANI GGCC 4 cut(s) 409, 543, 750, 909
BspEI TCCGGA 1 cut(s) 970
BspFNI CGCG 2 cut(s) 171, 709
BspLI GGNNCC 1 cut(s) 885
BspPI GGATC 2 cut(s) 233, 457
BspT107I GGYRCC 1 cut(s) 883
BsrBI CCGCTC 1 cut(s) 896
BsrI ACTGG 2 cut(s) 41, 953
BssECI CCNNGG 3 cut(s) 466, 670, 888
BssMI GATC 5 cut(s) 238, 462, 563, 772, 913
BssNI GRCGYC 1 cut(s) 583
BssT1I CCWWGG 3 cut(s) 466, 670, 888
Bst4CI ACNGT 6 cut(s) 34, 392, 497, 558, 740, 1013
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 583
BstBAI YACGTR 1 cut(s) 508
BstC8I GCNNGC 1 cut(s) 748
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 3 cut(s) 269, 541, 662
BstFNI CGCG 2 cut(s) 171, 709
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 5 cut(s) 241, 465, 566, 775, 916
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 5 cut(s) 238, 462, 563, 772, 913
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 2 cut(s) 151, 879
BstSLI GKGCMC 1 cut(s) 886
BstUI CGCG 2 cut(s) 171, 709
BstV1I GCAGC 1 cut(s) 703
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 4 cut(s) 409, 543, 750, 909
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 3 cut(s) 269, 541, 662
BtsIMutI CAGTG 5 cut(s) 96, 493, 677, 745, 831
Cac8I GCNNGC 1 cut(s) 748
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 113, 408
CseI GACGC 1 cut(s) 572
Csp6I GTAC 2 cut(s) 253, 1033
CviAII CATG 5 cut(s) 272, 640, 671, 700, 937
CviQI GTAC 2 cut(s) 253, 1033
DpnI GATC 5 cut(s) 240, 464, 565, 774, 915
DpnII GATC 5 cut(s) 238, 462, 563, 772, 913
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 466, 670, 888
Eco147I AGGCCT 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 645, 855
EcoICRI GAGCTC 1 cut(s) 64
EcoT14I CCWWGG 3 cut(s) 466, 670, 888
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 3 cut(s) 466, 670, 888
FaeI CATG 5 cut(s) 275, 643, 674, 703, 940
FaqI GGGAC 3 cut(s) 11, 89, 965
FatI CATG 5 cut(s) 271, 639, 670, 699, 936
FauI CCCGC 2 cut(s) 28, 164
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 1 cut(s) 586
Fnu4HI GCNGC 1 cut(s) 717
FokI GGATG 3 cut(s) 276, 548, 669
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 717
FspBI CTAG 3 cut(s) 182, 195, 431
GlaI GCGC 1 cut(s) 170
GluI GCNGC 1 cut(s) 717
HaeIII GGCC 4 cut(s) 409, 543, 750, 909
HapII CCGG 3 cut(s) 152, 880, 971
HgaI GACGC 1 cut(s) 572
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 1 cut(s) 583
Hin1II CATG 5 cut(s) 275, 643, 674, 703, 940
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 5 cut(s) 129, 175, 268, 290, 362
HpaII CCGG 3 cut(s) 152, 880, 971
HphI GGTGA 3 cut(s) 37, 183, 232
Hpy166II GTNNAC 4 cut(s) 587, 677, 705, 1035
Hpy188I TCNGA 5 cut(s) 16, 61, 690, 777, 1028
Hpy188III TCNNGA 3 cut(s) 294, 846, 971
Hpy8I GTNNAC 4 cut(s) 587, 677, 705, 1035
HpyAV CCTTC 5 cut(s) 98, 220, 451, 533, 1033
HpyCH4III ACNGT 6 cut(s) 34, 392, 497, 558, 740, 1013
HpyCH4IV ACGT 2 cut(s) 308, 507
HpyCH4V TGCA 5 cut(s) 329, 488, 806, 863, 940
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpySE526I ACGT 2 cut(s) 308, 507
Hsp92I GRCGYC 1 cut(s) 583
Hsp92II CATG 5 cut(s) 275, 643, 674, 703, 940
HspAI GCGC 1 cut(s) 169
Kpn2I TCCGGA 1 cut(s) 970
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 5 cut(s) 238, 462, 563, 772, 913
LmnI GCTCC 3 cut(s) 69, 893, 1050
Lsp1109I GCAGC 1 cut(s) 703
LweI GCATC 2 cut(s) 703, 793
MaeI CTAG 3 cut(s) 182, 195, 431
MaeII ACGT 2 cut(s) 308, 507
MaeIII GTNAC 4 cut(s) 34, 43, 189, 497
MalI GATC 5 cut(s) 240, 464, 565, 774, 915
MbiI CCGCTC 1 cut(s) 896
MboI GATC 5 cut(s) 238, 462, 563, 772, 913
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 3 cut(s) 66, 886, 1018
MluCI AATT 6 cut(s) 256, 369, 419, 819, 964, 999
MlyI GAGTC 1 cut(s) 277
MmeI TCCRAC 2 cut(s) 39, 626
MroI TCCGGA 1 cut(s) 970
MseI TTAA 3 cut(s) 311, 963, 998
MspI CCGG 3 cut(s) 152, 880, 971
MspR9I CCNGG 2 cut(s) 153, 881
MvnI CGCG 2 cut(s) 171, 709
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 2 cut(s) 153, 881
NcoI CCATGG 1 cut(s) 670
NdeII GATC 5 cut(s) 238, 462, 563, 772, 913
NlaIII CATG 5 cut(s) 275, 643, 674, 703, 940
NlaIV GGNNCC 1 cut(s) 885
NmuCI GTSAC 4 cut(s) 34, 43, 189, 497
NspI RCATGY 1 cut(s) 643
PceI AGGCCT 1 cut(s) 543
PciI ACATGT 1 cut(s) 639
PfeI GAWTC 4 cut(s) 129, 175, 290, 362
PfoI TCCNGGA 1 cut(s) 151
PkrI GCNGC 1 cut(s) 718
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Ppu21I YACGTR 1 cut(s) 508
PscI ACATGT 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 885
PspPI GGNCC 2 cut(s) 113, 408
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 2 cut(s) 254, 1034
RsaNI GTAC 2 cut(s) 253, 1033
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 3 cut(s) 311, 963, 998
SatI GCNGC 1 cut(s) 717
Sau3AI GATC 5 cut(s) 238, 462, 563, 772, 913
Sau96I GGNCC 2 cut(s) 113, 408
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 2 cut(s) 153, 881
SduI GDGCHC 3 cut(s) 66, 886, 1018
SfaNI GCATC 2 cut(s) 703, 793
SinI GGWCC 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
Sse9I AATT 6 cut(s) 256, 369, 419, 819, 964, 999
SseBI AGGCCT 1 cut(s) 543
SsiI CCGC 3 cut(s) 21, 171, 896
SspI AATATT 1 cut(s) 339
SspMI CTAG 3 cut(s) 182, 195, 431
SstI GAGCTC 1 cut(s) 66
StuI AGGCCT 1 cut(s) 543
StyD4I CCNGG 2 cut(s) 151, 879
StyI CCWWGG 3 cut(s) 466, 670, 888
TaaI ACNGT 6 cut(s) 34, 392, 497, 558, 740, 1013
TaiI ACGT 2 cut(s) 311, 510
TaqI TCGA 1 cut(s) 562
TasI AATT 6 cut(s) 256, 369, 419, 819, 964, 999
TatI WGTACW 1 cut(s) 1032
TfiI GAWTC 4 cut(s) 129, 175, 290, 362
Tru1I TTAA 3 cut(s) 311, 963, 998
Tru9I TTAA 3 cut(s) 311, 963, 998
TscAI CASTG 5 cut(s) 96, 500, 684, 745, 838
TseFI GTSAC 4 cut(s) 34, 43, 189, 497
TseI GCWGC 1 cut(s) 716
Tsp45I GTSAC 4 cut(s) 34, 43, 189, 497
TspDTI ATGAA 4 cut(s) 375, 552, 925, 971
TspRI CASTG 5 cut(s) 96, 500, 684, 745, 838
VpaK11BI GGWCC 1 cut(s) 113
XceI RCATGY 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 586
XspI CTAG 3 cut(s) 182, 195, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.