Rroxscaffold_2G00150650

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011025
Physical Location & Seq
Reverse (-)
88019000 .. 88024571
5572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00150650.1

Sequence Viewer

Length: 1428 bp
ATGCCGCCGACCACCGCCACCGTCCCCACTCTCACGGCCGTCACCATATCATACACGGAGCTCCAAGACAAGAAGACGGACTTGTCGAGGAAGATTGAACAAGGATTTGGGCCGAATGGGTTAGGGATTCTATCCGTCACTGACGTTCCGGGATTTTCATCCTTGCGCCGAAATCTTCTACATCTGTCACCTAGGTTGGCCAATCTTCCTGAAGAGGTGAAGAAGGAACTCGAAGATCCTCATAAGATATATAACTTGAGTACTCTTTATCTCCATCCATTTGCAGATATGTTAAAAGGATCTTTCTACGCAAATCCTATATTAGATAGACCAACAACAGATGAGGTTCTAATTCAAAGGTACCCATCGTATTGTGGATCAAATATATGGCCTAAGCGTGAGTTGCCAGAACTTGAAGTGGCCTTCAAAGCTCTGGGAAAGCTGATCCTTGAAGTTGGGCTAATGGTGGCATTTCACTGTGATAGATATGTATCAAAAGCAATTGAAATGCGTGAGGATGAAGGCCTTGAACAAGTACTTTTTCATTCTCGGTGTCATAAAGGGCGTCTGCTTTATTACTTTCCCACAAAAGAGAGCACTTCTACTGAAGACAGCGAAAACATGTCTTCTTGGTGTGGATGGCATACAGACCATGGTTCACTGACAGGTCTGACCTGTGCCGTGTTCATGAGAGATGCTGTTGAGATACCTTGCCCTGATAGTGCTGCTGGCCTTTATATTAGGACAAGAACTGATCAGATTGTCAGAGTTGTCTTTGGAGAAGATGAAATAGCATACCAAATTGGTGAAACCACAGAAGTTATGTCCAGAGGCTATCTTTGTGCAACACCTCATTGTGTCCGGGCACCCAAGGGAGAGGAAGCTTCTGGCCTTGACCGTTCAACATTTGCTTTCTTTATGCAACCAGACTGGGATGAAAAGCTTAATTTTCCTGAAGAAGTTCATATCCATAAAGAGTTGCATAGTGACCCTGAAGTAGGTACAAGATGCGTGAGGGATAGGAATGTAAACTTGGGGAAAGGAGATATGGATGGTGTGACATCGATGTCCAAGAGGGATGTGCCTATAAATGGGAGTGGACAAAACCAGAACCTCTTGAAGTTGAGTTCTTCAGCACTAAACTATCAAAATCCATTTTGTTTTTGTTTCCCACCAATCTTTGCCGGCTGCAACTATGTCGTCAGTTTCTTATTTATCTCAAGCACGGTCACACCAGAGCCGCCCCAAAAGCCACCGGAAAATCGGGTTCCGAAAGCGATGTGTGATGATGGCAAAGCACCAGAAAACAAGGTTCTACATCCTCGGACGCTGCGTTTCGATGCTCCTCTGCTGGCACGATCACTCCATATCGGATTAGAAATTGCGAAAAGTGCAAGTTCAGGAATCAGATCATGTGATAGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

475

Amino Acids

53.15

Weight (kDa)

5.83

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13400 AT4G13400
fragaria_vesca FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370
malus_domestica MD02G1062200.v1.1
prunus_persica Prupe.7G227200_v2.0.a1
pyrus_communis pycom02g04380
rosa_chinensis RchiOBHm_Chr2g0091051
rosa_laevigata RLG00000016171
rosa_multiflora Rmu_sc0005171.1_g000013
rosa_roxburghii Rroxscaffold_2G00150650
rosa_rugosa Rorug02G0011800 Rorug02G0011900
rosa_samantha Rh2AG058200 Rh2CG058800
rosa_wichuraiana Rw2G005100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 82, 1066
Acc65I GGTACC 1 cut(s) 360
AccB1I GGYRCC 2 cut(s) 360, 865
AciI CCGC 3 cut(s) 5, 15, 1241
AclWI GGATC 4 cut(s) 230, 307, 385, 439
AcoI YGGCCR 2 cut(s) 36, 198
AcuI CTGAAG 5 cut(s) 231, 627, 975, 1014, 1116
AcyI GRCGYC 1 cut(s) 565
AfaI GTAC 4 cut(s) 262, 362, 537, 1003
AfiI CCNNNNNNNGG 2 cut(s) 998, 1091
AflIII ACRYGT 1 cut(s) 621
AgsI TTSAA 9 cut(s) 98, 356, 416, 427, 452, 506, 530, 903, 1120
AjuI GAANNNNNNNTTGG 4 cut(s) 90, 122, 1016, 1048
AluBI AGCT 5 cut(s) 61, 431, 442, 884, 943
AluI AGCT 5 cut(s) 61, 431, 442, 884, 943
Alw21I GWGCWC 2 cut(s) 63, 599
AlwI GGATC 4 cut(s) 230, 307, 385, 439
AoxI GGCC 8 cut(s) 36, 110, 198, 389, 420, 523, 730, 889
ApeKI GCWGC 3 cut(s) 725, 1188, 1330
Asp700I GAANNNNTTC 1 cut(s) 960
Asp718I GGTACC 1 cut(s) 360
AspA2I CCTAGG 1 cut(s) 191
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 2 cut(s) 150, 863
AsuHPI GGTGA 4 cut(s) 34, 180, 229, 818
AvrII CCTAGG 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 868
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 2 cut(s) 360, 865
BanII GRGCYC 1 cut(s) 63
BbsI GAAGAC 3 cut(s) 80, 615, 618
Bbv12I GWGCWC 2 cut(s) 63, 599
BbvI GCAGC 3 cut(s) 712, 1175, 1317
BccI CCATC 5 cut(s) 282, 373, 633, 1046, 1283
BceAI ACGGC 3 cut(s) 23, 51, 665
BcgI CGANNNNNNTGC 2 cut(s) 1180, 1214
BclI TGATCA 1 cut(s) 754
BcnI CCSGG 2 cut(s) 150, 863
BfaI CTAG 1 cut(s) 192
BisI GCNGC 5 cut(s) 5, 726, 1189, 1241, 1331
BlnI CCTAGG 1 cut(s) 191
BlsI GCNGC 5 cut(s) 6, 727, 1190, 1242, 1332
BmcAI AGTACT 2 cut(s) 262, 537
Bme1390I CCNGG 2 cut(s) 150, 863
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 3 cut(s) 362, 867, 1269
BmrFI CCNGG 2 cut(s) 150, 863
BmrI ACTGGG 1 cut(s) 940
BmsI GCATC 3 cut(s) 685, 998, 1330
BmuI ACTGGG 1 cut(s) 940
BpiI GAAGAC 3 cut(s) 80, 615, 618
Bpu10I CCTNAGC 1 cut(s) 393
BpuEI CTTGAG 2 cut(s) 277, 1204
BpuMI CCSGG 2 cut(s) 150, 863
Bsa29I ATCGAT 1 cut(s) 1064
BsaBI GATNNNNATC 2 cut(s) 157, 490
BsaHI GRCGYC 1 cut(s) 565
BsaJI CCNNGG 4 cut(s) 191, 652, 870, 1322
BsaWI WCCGGW 1 cut(s) 1255
BsaXI ACNNNNNCTCC 2 cut(s) 1347, 1377
Bsc4I CCNNNNNNNGG 2 cut(s) 998, 1091
Bse118I RCCGGY 1 cut(s) 1184
Bse1I ACTGG 1 cut(s) 935
Bse8I GATNNNNATC 2 cut(s) 157, 490
BseCI ATCGAT 1 cut(s) 1064
BseDI CCNNGG 4 cut(s) 191, 652, 870, 1322
BseGI GGATG 8 cut(s) 158, 274, 523, 644, 940, 1057, 1084, 1318
BseJI GATNNNNATC 2 cut(s) 157, 490
BseLI CCNNNNNNNGG 2 cut(s) 998, 1091
BseNI ACTGG 1 cut(s) 935
BseRI GAGGAG 1 cut(s) 1335
BseSI GKGCMC 1 cut(s) 868
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 3 cut(s) 712, 1175, 1317
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 8 cut(s) 38, 112, 200, 391, 422, 525, 732, 891
BshNI GGYRCC 2 cut(s) 360, 865
BshVI ATCGAT 1 cut(s) 1064
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 2 cut(s) 63, 599
BsiSI CCGG 4 cut(s) 149, 862, 1185, 1256
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 2 cut(s) 998, 1091
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 8 cut(s) 38, 112, 200, 391, 422, 525, 732, 891
Bsp1286I GDGCHC 3 cut(s) 63, 599, 868
Bsp143I GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
Bsp19I CCATGG 1 cut(s) 652
BspACI CCGC 3 cut(s) 5, 15, 1241
BspANI GGCC 8 cut(s) 38, 112, 200, 391, 422, 525, 732, 891
BspDI ATCGAT 1 cut(s) 1064
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 3 cut(s) 362, 867, 1269
BspPI GGATC 4 cut(s) 230, 307, 385, 439
BspT107I GGYRCC 2 cut(s) 360, 865
BsrFI RCCGGY 1 cut(s) 1184
BsrI ACTGG 1 cut(s) 935
BssAI RCCGGY 1 cut(s) 1184
BssECI CCNNGG 4 cut(s) 191, 652, 870, 1322
BssMI GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
BssNI GRCGYC 1 cut(s) 565
BssT1I CCWWGG 3 cut(s) 191, 652, 870
Bst4CI ACNGT 4 cut(s) 22, 479, 899, 1228
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 565
BstC8I GCNNGC 3 cut(s) 730, 1186, 1353
BstDEI CTNAG 1 cut(s) 393
BstDSI CCRYGG 1 cut(s) 652
BstENI CCTNNNNNAGG 1 cut(s) 996
BstF5I GGATG 8 cut(s) 158, 274, 523, 644, 940, 1057, 1084, 1318
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 7 cut(s) 238, 302, 380, 447, 757, 1361, 1412
BstMBI GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 4 cut(s) 403, 428, 1249, 1391
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 2 cut(s) 148, 861
BstSLI GKGCMC 1 cut(s) 868
BstV1I GCAGC 3 cut(s) 712, 1175, 1317
BstV2I GAAGAC 3 cut(s) 80, 615, 618
BstX2I RGATCY 2 cut(s) 235, 299
BstYI RGATCY 2 cut(s) 235, 299
BstZI CGGCCG 1 cut(s) 36
Bsu15I ATCGAT 1 cut(s) 1064
BsuRI GGCC 8 cut(s) 38, 112, 200, 391, 422, 525, 732, 891
BsuTUI ATCGAT 1 cut(s) 1064
BtgI CCRYGG 1 cut(s) 652
BtgZI GCGATG 1 cut(s) 1292
BtsCI GGATG 8 cut(s) 158, 274, 523, 644, 940, 1057, 1084, 1318
BtsIMutI CAGTG 3 cut(s) 138, 475, 659
Cac8I GCNNGC 3 cut(s) 730, 1186, 1353
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 1184
Cfr13I GGNCC 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 1064
CseI GACGC 2 cut(s) 554, 1336
Csp6I GTAC 4 cut(s) 261, 361, 536, 1002
CviAII CATG 4 cut(s) 622, 653, 688, 1413
CviQI GTAC 4 cut(s) 261, 361, 536, 1002
DdeI CTNAG 1 cut(s) 393
DpnI GATC 7 cut(s) 237, 301, 379, 446, 756, 1360, 1411
DpnII GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
DrdI GACNNNNNNGTC 2 cut(s) 82, 1066
DseDI GACNNNNNNGTC 2 cut(s) 82, 1066
EaeI YGGCCR 2 cut(s) 36, 198
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 3 cut(s) 191, 652, 870
Eco147I AGGCCT 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 63
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 5 cut(s) 231, 627, 975, 1014, 1116
EcoICRI GAGCTC 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 996
EcoT14I CCWWGG 3 cut(s) 191, 652, 870
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 3 cut(s) 191, 652, 870
FaeI CATG 4 cut(s) 625, 656, 691, 1416
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 1 cut(s) 8
FatI CATG 4 cut(s) 621, 652, 687, 1412
FbaI TGATCA 1 cut(s) 754
Fnu4HI GCNGC 5 cut(s) 5, 726, 1189, 1241, 1331
FokI GGATG 8 cut(s) 145, 261, 530, 651, 947, 1064, 1091, 1305
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 5 cut(s) 5, 726, 1189, 1241, 1331
FspBI CTAG 1 cut(s) 192
GlaI GCGC 1 cut(s) 167
GluI GCNGC 5 cut(s) 5, 726, 1189, 1241, 1331
HaeIII GGCC 8 cut(s) 38, 112, 200, 391, 422, 525, 732, 891
HapII CCGG 4 cut(s) 149, 862, 1185, 1256
HgaI GACGC 2 cut(s) 554, 1336
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 565
Hin1II CATG 4 cut(s) 625, 656, 691, 1416
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HindIII AAGCTT 2 cut(s) 882, 941
HinfI GANTC 2 cut(s) 127, 1404
HpaII CCGG 4 cut(s) 149, 862, 1185, 1256
HphI GGTGA 4 cut(s) 34, 180, 229, 818
Hpy166II GTNNAC 3 cut(s) 659, 1030, 1100
Hpy188I TCNGA 7 cut(s) 672, 759, 767, 1272, 1326, 1373, 1409
Hpy188III TCNNGA 6 cut(s) 209, 688, 828, 953, 1117, 1401
Hpy8I GTNNAC 3 cut(s) 659, 1030, 1100
HpyAV CCTTC 3 cut(s) 217, 433, 515
HpyCH4III ACNGT 4 cut(s) 22, 479, 899, 1228
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 6 cut(s) 284, 845, 922, 982, 1191, 1394
HpyF10VI GCNNNNNNNGC 4 cut(s) 403, 428, 1249, 1391
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 565
Hsp92II CATG 4 cut(s) 625, 656, 691, 1416
HspAI GCGC 1 cut(s) 166
KpnI GGTACC 1 cut(s) 364
KroI GCCGGC 1 cut(s) 1184
KroNI GCCGGC 1 cut(s) 1186
Ksp22I TGATCA 1 cut(s) 754
Kzo9I GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
LmnI GCTCC 3 cut(s) 58, 66, 1348
Lsp1109I GCAGC 3 cut(s) 712, 1175, 1317
LweI GCATC 3 cut(s) 685, 998, 1330
MaeI CTAG 1 cut(s) 192
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 6 cut(s) 40, 136, 186, 986, 1057, 1228
MalI GATC 7 cut(s) 237, 301, 379, 446, 756, 1360, 1411
MboI GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 2 cut(s) 235, 299
MhlI GDGCHC 3 cut(s) 63, 599, 868
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 5 cut(s) 351, 501, 801, 946, 1380
MluNI TGGCCA 1 cut(s) 200
Mox20I TGGCCA 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 1184
MroXI GAANNNNTTC 1 cut(s) 960
MscI TGGCCA 1 cut(s) 200
MseI TTAA 2 cut(s) 293, 945
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 4 cut(s) 149, 862, 1185, 1256
MspR9I CCNGG 2 cut(s) 150, 863
MunI CAATTG 1 cut(s) 501
MwoI GCNNNNNNNGC 4 cut(s) 403, 428, 1249, 1391
NaeI GCCGGC 1 cut(s) 1186
NciI CCSGG 2 cut(s) 150, 863
NcoI CCATGG 1 cut(s) 652
NdeII GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
NgoMIV GCCGGC 1 cut(s) 1184
NlaIII CATG 4 cut(s) 625, 656, 691, 1416
NlaIV GGNNCC 3 cut(s) 362, 867, 1269
NmuCI GTSAC 6 cut(s) 40, 136, 186, 986, 1057, 1228
NspI RCATGY 1 cut(s) 625
PagI TCATGA 1 cut(s) 687
PceI AGGCCT 1 cut(s) 525
PciI ACATGT 1 cut(s) 621
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 1330
PdiI GCCGGC 1 cut(s) 1186
PdmI GAANNNNTTC 1 cut(s) 960
PfeI GAWTC 2 cut(s) 127, 1404
PfoI TCCNGGA 1 cut(s) 148
PkrI GCNGC 5 cut(s) 6, 727, 1190, 1242, 1332
PscI ACATGT 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 63
PspN4I GGNNCC 3 cut(s) 362, 867, 1269
PspPI GGNCC 1 cut(s) 110
PsuI RGATCY 2 cut(s) 235, 299
RsaI GTAC 4 cut(s) 262, 362, 537, 1003
RsaNI GTAC 4 cut(s) 261, 361, 536, 1002
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 2 cut(s) 293, 945
SatI GCNGC 5 cut(s) 5, 726, 1189, 1241, 1331
Sau3AI GATC 7 cut(s) 235, 299, 377, 444, 754, 1358, 1409
Sau96I GGNCC 1 cut(s) 110
ScaI AGTACT 2 cut(s) 262, 537
ScrFI CCNGG 2 cut(s) 150, 863
SduI GDGCHC 3 cut(s) 63, 599, 868
SfaNI GCATC 3 cut(s) 685, 998, 1330
SmlI CTYRAG 2 cut(s) 256, 1219
SmoI CTYRAG 2 cut(s) 256, 1219
Sse9I AATT 5 cut(s) 351, 501, 801, 946, 1380
SseBI AGGCCT 1 cut(s) 525
SsiI CCGC 3 cut(s) 5, 15, 1241
SspMI CTAG 1 cut(s) 192
SstI GAGCTC 1 cut(s) 63
StuI AGGCCT 1 cut(s) 525
StyD4I CCNGG 2 cut(s) 148, 861
StyI CCWWGG 3 cut(s) 191, 652, 870
TaaI ACNGT 4 cut(s) 22, 479, 899, 1228
TaiI ACGT 1 cut(s) 147
TaqI TCGA 4 cut(s) 86, 231, 1064, 1338
TasI AATT 5 cut(s) 351, 501, 801, 946, 1380
TatI WGTACW 2 cut(s) 260, 535
TauI GCSGC 2 cut(s) 7, 1243
TfiI GAWTC 2 cut(s) 127, 1404
Tru1I TTAA 2 cut(s) 293, 945
Tru9I TTAA 2 cut(s) 293, 945
TscAI CASTG 3 cut(s) 145, 482, 666
TseFI GTSAC 6 cut(s) 40, 136, 186, 986, 1057, 1228
TseI GCWGC 3 cut(s) 725, 1188, 1330
Tsp45I GTSAC 6 cut(s) 40, 136, 186, 986, 1057, 1228
TspDTI ATGAA 7 cut(s) 147, 533, 534, 676, 801, 951, 953
TspGWI ACGGA 3 cut(s) 71, 92, 124
TspRI CASTG 3 cut(s) 145, 482, 666
XagI CCTNNNNNAGG 1 cut(s) 996
XceI RCATGY 1 cut(s) 625
XmaJI CCTAGG 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 960
XspI CTAG 1 cut(s) 192
ZrmI AGTACT 2 cut(s) 262, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.