RLG00000016171

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011025
Physical Location & Seq
Forward (+)
4423641 .. 4428429
4789 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016171

Sequence Viewer

Length: 1071 bp
ATGCCGCCGACCACCACCGTCTCCACTCTCACGGCCGTCACCATATCATACACGGAGCTCCAAGACAAGACGACGGACTTGTCGAGGAAGATTGAAGAAGGATTTGGGCCGAATGGGTTAGGGATTCTATCCGTCACTGATGTTCCGGGATTTTCATCCTTGCGCCAAAATCTTCTACATCTGTCACCTAGGTTGGCCAATCTTCCTGAAGAGGTGAAGAAGGAACTCGAAGATCCTCATAGTAGGTATAATTTTGGATGGAGTCATGGAAAGGAGAAGTTGGAATCTGGAAAGCCAGATATGTTAAAAGGATCTTTCTACGCAAATCCTATATTAGATAGACCAACAACAGATGAGGCTCTAATTCAAAGGTACCCATCGTATTGTGGATCAAATATATGGCCTAAGCGTGAGTTGCCAGAACTTGAAGTGGCCTTCAAAGCTCTGGGAAAGCTGATCCTTGAAGTTGGGCTTATGGTGGCATTTCACTGTGATAGATATGTATCAAAAGCAATTGAAATGCGTGAGGATGAAGGCCTTGAACAAGTACTTTTTCATTCTCGGTGTCATAAAGGGCGTCTGCTTTATTACTTTCCCACAAAAGAGAGCACTCCTACTGAAGATAGCGAAAACATGTCTTCTTGGTGTGGATGGCATACAGACCATGGTTCACTGACAGGTCTGACCTGTGCCATGTTCATGAGAGATGCTGTTGAGATACCTTGCCCTGATAGTGCTGCTGGCCTTTATATTAGGACAAGAACTGATCAGATTGTCAGAGTTGTCTTTGGAGAAGATGAAATAGCATACCAAATTGGTGAAACCACAGAAGTCATGTCCAGAGGCTATCTTTGTGCAACACCTCATTGTGTCCGGGCACCCAAGGGAGAGGAAGCTTCTGGCCTTGACCGTTCAACATTTGCTTTCTTTATGCAGCCAGATTGGGATGAAAAGCTTAATTTTCCTGAAGAAGTTCATATCCATAAAGAGTTGATCCCACCAAATGGTGCTCTCACCTTTGGAGAGTACACAGAGAAGCTCCTCGACAAATACTACCACCTCAAAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

357

Amino Acids

40.3

Weight (kDa)

5.31

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 22 - 136 9e-06 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13400 AT4G13400
fragaria_vesca FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370
malus_domestica MD02G1062200.v1.1
prunus_persica Prupe.7G227200_v2.0.a1
pyrus_communis pycom02g04380
rosa_chinensis RchiOBHm_Chr2g0091051
rosa_laevigata RLG00000016171
rosa_multiflora Rmu_sc0005171.1_g000013
rosa_roxburghii Rroxscaffold_2G00150650
rosa_rugosa Rorug02G0011800 Rorug02G0011900
rosa_samantha Rh2AG058200 Rh2CG058800
rosa_wichuraiana Rw2G005100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 79
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 2 cut(s) 372, 877
AccB7I CCANNNNNTGG 1 cut(s) 1004
AciI CCGC 1 cut(s) 5
AclWI GGATC 5 cut(s) 227, 319, 397, 451, 988
AcoI YGGCCR 2 cut(s) 33, 195
AcuI CTGAAG 3 cut(s) 228, 639, 987
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 3 cut(s) 374, 549, 1028
AfiI CCNNNNNNNGG 1 cut(s) 1004
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 8 cut(s) 95, 368, 428, 439, 464, 518, 542, 915
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 6 cut(s) 58, 443, 454, 896, 955, 1039
AluI AGCT 6 cut(s) 58, 443, 454, 896, 955, 1039
Alw21I GWGCWC 3 cut(s) 60, 611, 1012
Alw26I GTCTC 1 cut(s) 25
AlwI GGATC 5 cut(s) 227, 319, 397, 451, 988
AoxI GGCC 8 cut(s) 33, 107, 195, 401, 432, 535, 742, 901
ApeKI GCWGC 2 cut(s) 737, 934
Asp700I GAANNNNTTC 1 cut(s) 972
Asp718I GGTACC 1 cut(s) 372
AspA2I CCTAGG 1 cut(s) 188
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 2 cut(s) 147, 875
AsuHPI GGTGA 5 cut(s) 31, 177, 226, 830, 1006
AvrII CCTAGG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 880
BalI TGGCCA 1 cut(s) 197
BanI GGYRCC 2 cut(s) 372, 877
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 3 cut(s) 60, 611, 1012
BbvI GCAGC 2 cut(s) 724, 946
BccI CCATC 3 cut(s) 252, 385, 645
BceAI ACGGC 2 cut(s) 20, 48
BclI TGATCA 1 cut(s) 766
BcnI CCSGG 2 cut(s) 147, 875
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 1 cut(s) 189
BisI GCNGC 3 cut(s) 5, 738, 935
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 3 cut(s) 6, 739, 936
BmcAI AGTACT 1 cut(s) 549
Bme1390I CCNGG 2 cut(s) 147, 875
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 2 cut(s) 374, 879
BmrFI CCNGG 2 cut(s) 147, 875
BmsI GCATC 1 cut(s) 697
BpiI GAAGAC 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 2 cut(s) 147, 875
BsaBI GATNNNNATC 2 cut(s) 154, 502
BsaHI GRCGYC 1 cut(s) 577
BsaJI CCNNGG 3 cut(s) 188, 664, 882
Bsc4I CCNNNNNNNGG 1 cut(s) 1004
Bse8I GATNNNNATC 2 cut(s) 154, 502
BseDI CCNNGG 3 cut(s) 188, 664, 882
BseGI GGATG 5 cut(s) 155, 263, 535, 656, 952
BseJI GATNNNNATC 2 cut(s) 154, 502
BseLI CCNNNNNNNGG 1 cut(s) 1004
BseRI GAGGAG 1 cut(s) 1031
BseSI GKGCMC 1 cut(s) 880
BseX3I CGGCCG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 724, 946
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
BshNI GGYRCC 2 cut(s) 372, 877
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 3 cut(s) 60, 611, 1012
BsiSI CCGG 2 cut(s) 146, 874
BslI CCNNNNNNNGG 1 cut(s) 1004
BsmAI GTCTC 1 cut(s) 25
BsmBI CGTCTC 1 cut(s) 25
BsnI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
Bsp1286I GDGCHC 4 cut(s) 60, 611, 880, 1012
Bsp143I GATC 6 cut(s) 232, 311, 389, 456, 766, 993
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 1 cut(s) 5
BspANI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 2 cut(s) 374, 879
BspPI GGATC 5 cut(s) 227, 319, 397, 451, 988
BspT107I GGYRCC 2 cut(s) 372, 877
BssECI CCNNGG 3 cut(s) 188, 664, 882
BssMI GATC 6 cut(s) 232, 311, 389, 456, 766, 993
BssNI GRCGYC 1 cut(s) 577
BssT1I CCWWGG 3 cut(s) 188, 664, 882
Bst4CI ACNGT 3 cut(s) 19, 491, 911
Bst6I CTCTTC 1 cut(s) 204
BstACI GRCGYC 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 742
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 1 cut(s) 664
BstF5I GGATG 5 cut(s) 155, 263, 535, 656, 952
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 6 cut(s) 235, 314, 392, 459, 769, 996
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 6 cut(s) 232, 311, 389, 456, 766, 993
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 2 cut(s) 415, 440
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 2 cut(s) 145, 873
BstSLI GKGCMC 1 cut(s) 880
BstV1I GCAGC 2 cut(s) 724, 946
BstV2I GAAGAC 1 cut(s) 630
BstX2I RGATCY 2 cut(s) 232, 311
BstYI RGATCY 2 cut(s) 232, 311
BstZI CGGCCG 1 cut(s) 33
BsuRI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
BtgI CCRYGG 1 cut(s) 664
BtsCI GGATG 5 cut(s) 155, 263, 535, 656, 952
BtsIMutI CAGTG 3 cut(s) 135, 487, 671
Cac8I GCNNGC 1 cut(s) 742
CciI TCATGA 1 cut(s) 699
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 107
CseI GACGC 1 cut(s) 566
Csp6I GTAC 3 cut(s) 373, 548, 1027
CviAII CATG 6 cut(s) 266, 634, 665, 694, 700, 835
CviQI GTAC 3 cut(s) 373, 548, 1027
DdeI CTNAG 1 cut(s) 405
DpnI GATC 6 cut(s) 234, 313, 391, 458, 768, 995
DpnII GATC 6 cut(s) 232, 311, 389, 456, 766, 993
DrdI GACNNNNNNGTC 1 cut(s) 79
DseDI GACNNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 2 cut(s) 33, 195
EagI CGGCCG 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 58
EclXI CGGCCG 1 cut(s) 33
Eco130I CCWWGG 3 cut(s) 188, 664, 882
Eco147I AGGCCT 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 60
Eco52I CGGCCG 1 cut(s) 33
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 228, 639, 987
EcoICRI GAGCTC 1 cut(s) 58
EcoT14I CCWWGG 3 cut(s) 188, 664, 882
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 3 cut(s) 188, 664, 882
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 6 cut(s) 269, 637, 668, 697, 703, 838
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FatI CATG 6 cut(s) 265, 633, 664, 693, 699, 834
FbaI TGATCA 1 cut(s) 766
Fnu4HI GCNGC 3 cut(s) 5, 738, 935
FokI GGATG 5 cut(s) 142, 270, 542, 663, 959
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 3 cut(s) 5, 738, 935
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 164
GluI GCNGC 3 cut(s) 5, 738, 935
HaeIII GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
HapII CCGG 2 cut(s) 146, 874
HgaI GACGC 1 cut(s) 566
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 6 cut(s) 269, 637, 668, 697, 703, 838
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HindIII AAGCTT 2 cut(s) 894, 953
HinfI GANTC 3 cut(s) 124, 262, 284
HpaII CCGG 2 cut(s) 146, 874
HphI GGTGA 5 cut(s) 31, 177, 226, 830, 1006
Hpy166II GTNNAC 2 cut(s) 671, 1029
Hpy188I TCNGA 3 cut(s) 684, 771, 779
Hpy188III TCNNGA 5 cut(s) 206, 288, 700, 840, 965
Hpy8I GTNNAC 2 cut(s) 671, 1029
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 4 cut(s) 92, 214, 445, 527
HpyCH4III ACNGT 3 cut(s) 19, 491, 911
HpyCH4V TGCA 2 cut(s) 857, 934
HpyF10VI GCNNNNNNNGC 2 cut(s) 415, 440
HpyF3I CTNAG 1 cut(s) 405
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 6 cut(s) 269, 637, 668, 697, 703, 838
HspAI GCGC 1 cut(s) 163
KpnI GGTACC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 6 cut(s) 232, 311, 389, 456, 766, 993
LmnI GCTCC 3 cut(s) 55, 63, 1044
Lsp1109I GCAGC 2 cut(s) 724, 946
LweI GCATC 1 cut(s) 697
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 37, 133, 183
MalI GATC 6 cut(s) 234, 313, 391, 458, 768, 995
MboI GATC 6 cut(s) 232, 311, 389, 456, 766, 993
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 232, 311
MhlI GDGCHC 4 cut(s) 60, 611, 880, 1012
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 5 cut(s) 250, 363, 513, 813, 958
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 261
Mox20I TGGCCA 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 972
MscI TGGCCA 1 cut(s) 197
MseI TTAA 2 cut(s) 305, 957
MslI CAYNNNNRTG 1 cut(s) 698
Msp20I TGGCCA 1 cut(s) 197
MspI CCGG 2 cut(s) 146, 874
MspR9I CCNGG 2 cut(s) 147, 875
MunI CAATTG 1 cut(s) 513
MwoI GCNNNNNNNGC 2 cut(s) 415, 440
NciI CCSGG 2 cut(s) 147, 875
NcoI CCATGG 1 cut(s) 664
NdeII GATC 6 cut(s) 232, 311, 389, 456, 766, 993
NlaIII CATG 6 cut(s) 269, 637, 668, 697, 703, 838
NlaIV GGNNCC 2 cut(s) 374, 879
NmuCI GTSAC 3 cut(s) 37, 133, 183
NspI RCATGY 1 cut(s) 637
PagI TCATGA 1 cut(s) 699
PceI AGGCCT 1 cut(s) 537
PciI ACATGT 1 cut(s) 633
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 2 cut(s) 124, 284
PflMI CCANNNNNTGG 1 cut(s) 1004
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 3 cut(s) 6, 739, 936
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PscI ACATGT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 60
PspN4I GGNNCC 2 cut(s) 374, 879
PspPI GGNCC 1 cut(s) 107
PsuI RGATCY 2 cut(s) 232, 311
RsaI GTAC 3 cut(s) 374, 549, 1028
RsaNI GTAC 3 cut(s) 373, 548, 1027
RseI CAYNNNNRTG 1 cut(s) 698
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 305, 957
SatI GCNGC 3 cut(s) 5, 738, 935
Sau3AI GATC 6 cut(s) 232, 311, 389, 456, 766, 993
Sau96I GGNCC 1 cut(s) 107
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 2 cut(s) 147, 875
SduI GDGCHC 4 cut(s) 60, 611, 880, 1012
SfaNI GCATC 1 cut(s) 697
SmiMI CAYNNNNRTG 1 cut(s) 698
Sse9I AATT 5 cut(s) 250, 363, 513, 813, 958
SseBI AGGCCT 1 cut(s) 537
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 189
SstI GAGCTC 1 cut(s) 60
StuI AGGCCT 1 cut(s) 537
StyD4I CCNGG 2 cut(s) 145, 873
StyI CCWWGG 3 cut(s) 188, 664, 882
TaaI ACNGT 3 cut(s) 19, 491, 911
TaqI TCGA 3 cut(s) 83, 228, 1044
TasI AATT 5 cut(s) 250, 363, 513, 813, 958
TatI WGTACW 2 cut(s) 547, 1026
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 2 cut(s) 124, 284
Tru1I TTAA 2 cut(s) 305, 957
Tru9I TTAA 2 cut(s) 305, 957
TscAI CASTG 3 cut(s) 142, 494, 678
TseFI GTSAC 3 cut(s) 37, 133, 183
TseI GCWGC 2 cut(s) 737, 934
Tsp45I GTSAC 3 cut(s) 37, 133, 183
TspDTI ATGAA 7 cut(s) 144, 545, 546, 688, 813, 963, 965
TspGWI ACGGA 3 cut(s) 68, 89, 121
TspRI CASTG 3 cut(s) 142, 494, 678
Van91I CCANNNNNTGG 1 cut(s) 1004
XceI RCATGY 1 cut(s) 637
XmaJI CCTAGG 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 1 cut(s) 189
ZrmI AGTACT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.