Rh2AG058200

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0011025
Physical Location & Seq
Forward (+)
4705908 .. 4710365
4458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG058200.1

Sequence Viewer

Length: 948 bp
ATGCCGCCGACCACCGCGGTCCCCACTCTCACGGCCGTCACCATATCATACACGGAGCTCCAAGACAAGAAGACGGACTTGTCGAGGAAGATTGAACAAGGATTTGGGCCGAATGGGTTAGGGATTCTATCCGTCACTGATGTTCCGGGATTTTCATCCTTGCGCCAAAATCTTCTACATCTGTCACCTAGGTTGGCCAATCTTCCTGAAGAGGTGAAGAAGGAACTCGAAGATCCTCATAGTAGGTATAATTTTGGATGGAGTCATGGAAAGGAGAAGTTGGAATCTGGAAAGCCAGATATGTTAAAAGGATCTTTCTACGCTAATCCTATATTAGATAGACCAACAACAGATGAGGCTCTAATTCAAAGGTACCCATCGTATTGTGGATCAAATATATGGCCTAAGCGTGAGTTGCCAGAACTTGAAGTGGCCTTCAAAGCTCTGGGAAAGCTGATCCTTGAAGTTGGGCTTATGGTGGCATTTCACTGTGATAGATATGTATCAAAAGCAATTGAAATGCGTGAGGATGAAGGCCTTGAACAAGTACTTTTTCATTCTCGGTGTCATAAAGGGCGTCTGCTTTATTACTTTCCCACAAAAGAGAGCACTCCTACTGAAGATAGCGAAAACATGTCTTCTTGGTGTGGATGGCATACAGACCATGGTTCACTGACAGGTCTGACCTGTGCCATGTTCATGAGAGATGCTGTTGAGATACCTTGCCCTGATAGTGCTGCTGGCCTTTATATTAGGACAAGAACTGATCAGATTGTCAGAGTTATCTTTGGAGAAGATGAAATAGCATACCAAATTGGTGAAACCACAGAAGTTATGTCCAGAGGCTATCTTTGTGCAACACCTCATTGTGTCCGGGCACCCAAGGGAGAGGAAGCTTCTGGCCTTGACCTTTCAACATTTGCTTTCTTTATGCAACCAGACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

315

Amino Acids

35.34

Weight (kDa)

5.36

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 21 - 136 2.9e-06 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13400 AT4G13400
fragaria_vesca FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370 FvH4_1g05370
malus_domestica MD02G1062200.v1.1
prunus_persica Prupe.7G227200_v2.0.a1
pyrus_communis pycom02g04380
rosa_chinensis RchiOBHm_Chr2g0091051
rosa_laevigata RLG00000016171
rosa_multiflora Rmu_sc0005171.1_g000013
rosa_roxburghii Rroxscaffold_2G00150650
rosa_rugosa Rorug02G0011800 Rorug02G0011900
rosa_samantha Rh2AG058200 Rh2CG058800
rosa_wichuraiana Rw2G005100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 79
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 2 cut(s) 372, 877
AccII CGCG 1 cut(s) 17
AciI CCGC 3 cut(s) 5, 15, 17
AclWI GGATC 4 cut(s) 227, 319, 397, 451
AcoI YGGCCR 2 cut(s) 33, 195
AcuI CTGAAG 2 cut(s) 228, 639
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 2 cut(s) 374, 549
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 8 cut(s) 95, 368, 428, 439, 464, 518, 542, 915
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 4 cut(s) 58, 443, 454, 896
AluI AGCT 4 cut(s) 58, 443, 454, 896
Alw21I GWGCWC 2 cut(s) 60, 611
AlwI GGATC 4 cut(s) 227, 319, 397, 451
AoxI GGCC 8 cut(s) 33, 107, 195, 401, 432, 535, 742, 901
ApeKI GCWGC 1 cut(s) 737
Asp718I GGTACC 1 cut(s) 372
AspA2I CCTAGG 1 cut(s) 188
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 19, 107
AsuC2I CCSGG 2 cut(s) 147, 875
AsuHPI GGTGA 4 cut(s) 31, 177, 226, 830
AvaII GGWCC 1 cut(s) 19
AvrII CCTAGG 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 880
BalI TGGCCA 1 cut(s) 197
BanI GGYRCC 2 cut(s) 372, 877
BanII GRGCYC 1 cut(s) 60
BbsI GAAGAC 2 cut(s) 77, 630
Bbv12I GWGCWC 2 cut(s) 60, 611
BbvI GCAGC 1 cut(s) 724
BccI CCATC 3 cut(s) 252, 385, 645
BceAI ACGGC 2 cut(s) 20, 48
BclI TGATCA 1 cut(s) 766
BcnI CCSGG 2 cut(s) 147, 875
BfaI CTAG 1 cut(s) 189
BisI GCNGC 2 cut(s) 5, 738
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 2 cut(s) 6, 739
BmcAI AGTACT 1 cut(s) 549
Bme1390I CCNGG 2 cut(s) 147, 875
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 19, 107
BmiI GGNNCC 3 cut(s) 21, 374, 879
BmrFI CCNGG 2 cut(s) 147, 875
BmsI GCATC 1 cut(s) 697
BpiI GAAGAC 2 cut(s) 77, 630
Bpu10I CCTNAGC 1 cut(s) 405
BpuMI CCSGG 2 cut(s) 147, 875
BsaBI GATNNNNATC 2 cut(s) 154, 502
BsaHI GRCGYC 1 cut(s) 577
BsaJI CCNNGG 4 cut(s) 15, 188, 664, 882
Bse8I GATNNNNATC 2 cut(s) 154, 502
BseDI CCNNGG 4 cut(s) 15, 188, 664, 882
BseGI GGATG 4 cut(s) 155, 263, 535, 656
BseJI GATNNNNATC 2 cut(s) 154, 502
BseSI GKGCMC 1 cut(s) 880
BseX3I CGGCCG 1 cut(s) 33
BseXI GCAGC 1 cut(s) 724
Bsh1236I CGCG 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
BshNI GGYRCC 2 cut(s) 372, 877
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 60, 611
BsiSI CCGG 2 cut(s) 146, 874
BslFI GGGAC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 5
BsnI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
Bsp1286I GDGCHC 3 cut(s) 60, 611, 880
Bsp143I GATC 5 cut(s) 232, 311, 389, 456, 766
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 3 cut(s) 5, 15, 17
BspANI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 3 cut(s) 21, 374, 879
BspPI GGATC 4 cut(s) 227, 319, 397, 451
BspT107I GGYRCC 2 cut(s) 372, 877
BssECI CCNNGG 4 cut(s) 15, 188, 664, 882
BssMI GATC 5 cut(s) 232, 311, 389, 456, 766
BssNI GRCGYC 1 cut(s) 577
BssT1I CCWWGG 3 cut(s) 188, 664, 882
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 1 cut(s) 204
BstACI GRCGYC 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 742
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 2 cut(s) 15, 664
BstF5I GGATG 4 cut(s) 155, 263, 535, 656
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 5 cut(s) 235, 314, 392, 459, 769
BstMBI GATC 5 cut(s) 232, 311, 389, 456, 766
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 2 cut(s) 415, 440
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 2 cut(s) 145, 873
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 724
BstV2I GAAGAC 2 cut(s) 77, 630
BstX2I RGATCY 2 cut(s) 232, 311
BstYI RGATCY 2 cut(s) 232, 311
BstZI CGGCCG 1 cut(s) 33
BsuRI GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
BtgI CCRYGG 2 cut(s) 15, 664
BtsCI GGATG 4 cut(s) 155, 263, 535, 656
BtsIMutI CAGTG 3 cut(s) 135, 487, 671
Cac8I GCNNGC 1 cut(s) 742
CciI TCATGA 1 cut(s) 699
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 19, 107
Cfr42I CCGCGG 1 cut(s) 18
CseI GACGC 1 cut(s) 566
Csp6I GTAC 2 cut(s) 373, 548
CviAII CATG 5 cut(s) 266, 634, 665, 694, 700
CviQI GTAC 2 cut(s) 373, 548
DdeI CTNAG 1 cut(s) 405
DpnI GATC 5 cut(s) 234, 313, 391, 458, 768
DpnII GATC 5 cut(s) 232, 311, 389, 456, 766
DrdI GACNNNNNNGTC 1 cut(s) 79
DseDI GACNNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 2 cut(s) 33, 195
EagI CGGCCG 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 58
EclXI CGGCCG 1 cut(s) 33
Eco130I CCWWGG 3 cut(s) 188, 664, 882
Eco147I AGGCCT 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 19
Eco52I CGGCCG 1 cut(s) 33
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 2 cut(s) 228, 639
EcoICRI GAGCTC 1 cut(s) 58
EcoT14I CCWWGG 3 cut(s) 188, 664, 882
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 3 cut(s) 188, 664, 882
FaeI CATG 5 cut(s) 269, 637, 668, 697, 703
FalI AAGNNNNNCTT 4 cut(s) 62, 94, 456, 488
FaqI GGGAC 1 cut(s) 5
FatI CATG 5 cut(s) 265, 633, 664, 693, 699
FbaI TGATCA 1 cut(s) 766
Fnu4HI GCNGC 2 cut(s) 5, 738
FokI GGATG 4 cut(s) 142, 270, 542, 663
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 2 cut(s) 5, 738
FspBI CTAG 1 cut(s) 189
GlaI GCGC 1 cut(s) 164
GluI GCNGC 2 cut(s) 5, 738
HaeIII GGCC 8 cut(s) 35, 109, 197, 403, 434, 537, 744, 903
HapII CCGG 2 cut(s) 146, 874
HgaI GACGC 1 cut(s) 566
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 5 cut(s) 269, 637, 668, 697, 703
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HindIII AAGCTT 1 cut(s) 894
HinfI GANTC 3 cut(s) 124, 262, 284
HpaII CCGG 2 cut(s) 146, 874
HphI GGTGA 4 cut(s) 31, 177, 226, 830
Hpy166II GTNNAC 1 cut(s) 671
Hpy188I TCNGA 3 cut(s) 684, 771, 779
Hpy188III TCNNGA 4 cut(s) 206, 288, 700, 840
Hpy8I GTNNAC 1 cut(s) 671
HpyAV CCTTC 3 cut(s) 214, 445, 527
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4V TGCA 2 cut(s) 857, 934
HpyF10VI GCNNNNNNNGC 2 cut(s) 415, 440
HpyF3I CTNAG 1 cut(s) 405
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 5 cut(s) 269, 637, 668, 697, 703
HspAI GCGC 1 cut(s) 163
KpnI GGTACC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 766
KspI CCGCGG 1 cut(s) 18
Kzo9I GATC 5 cut(s) 232, 311, 389, 456, 766
LmnI GCTCC 2 cut(s) 55, 63
Lsp1109I GCAGC 1 cut(s) 724
LweI GCATC 1 cut(s) 697
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 37, 133, 183
MalI GATC 5 cut(s) 234, 313, 391, 458, 768
MboI GATC 5 cut(s) 232, 311, 389, 456, 766
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 232, 311
MhlI GDGCHC 3 cut(s) 60, 611, 880
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 4 cut(s) 250, 363, 513, 813
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 8 cut(s) 78, 205, 246, 349, 520, 836, 873, 883
Mox20I TGGCCA 1 cut(s) 197
MscI TGGCCA 1 cut(s) 197
MseI TTAA 1 cut(s) 305
MslI CAYNNNNRTG 1 cut(s) 698
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 146, 874
MspR9I CCNGG 2 cut(s) 147, 875
MunI CAATTG 1 cut(s) 513
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 415, 440
NciI CCSGG 2 cut(s) 147, 875
NcoI CCATGG 1 cut(s) 664
NdeII GATC 5 cut(s) 232, 311, 389, 456, 766
NlaIII CATG 5 cut(s) 269, 637, 668, 697, 703
NlaIV GGNNCC 3 cut(s) 21, 374, 879
NmuCI GTSAC 3 cut(s) 37, 133, 183
NspI RCATGY 1 cut(s) 637
PagI TCATGA 1 cut(s) 699
PceI AGGCCT 1 cut(s) 537
PciI ACATGT 1 cut(s) 633
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PfeI GAWTC 2 cut(s) 124, 284
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 2 cut(s) 6, 739
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PscI ACATGT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 60
PspN4I GGNNCC 3 cut(s) 21, 374, 879
PspPI GGNCC 2 cut(s) 19, 107
PsuI RGATCY 2 cut(s) 232, 311
RsaI GTAC 2 cut(s) 374, 549
RsaNI GTAC 2 cut(s) 373, 548
RseI CAYNNNNRTG 1 cut(s) 698
SacI GAGCTC 1 cut(s) 60
SacII CCGCGG 1 cut(s) 18
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 2 cut(s) 5, 738
Sau3AI GATC 5 cut(s) 232, 311, 389, 456, 766
Sau96I GGNCC 2 cut(s) 19, 107
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 2 cut(s) 147, 875
SduI GDGCHC 3 cut(s) 60, 611, 880
SfaNI GCATC 1 cut(s) 697
Sfr303I CCGCGG 1 cut(s) 18
SgrBI CCGCGG 1 cut(s) 18
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 698
Sse9I AATT 4 cut(s) 250, 363, 513, 813
SseBI AGGCCT 1 cut(s) 537
SsiI CCGC 3 cut(s) 5, 15, 17
SspMI CTAG 1 cut(s) 189
SstI GAGCTC 1 cut(s) 60
StuI AGGCCT 1 cut(s) 537
StyD4I CCNGG 2 cut(s) 145, 873
StyI CCWWGG 3 cut(s) 188, 664, 882
TaaI ACNGT 1 cut(s) 491
TaqI TCGA 2 cut(s) 83, 228
TasI AATT 4 cut(s) 250, 363, 513, 813
TatI WGTACW 1 cut(s) 547
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 2 cut(s) 124, 284
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 3 cut(s) 142, 494, 678
TseFI GTSAC 3 cut(s) 37, 133, 183
TseI GCWGC 1 cut(s) 737
Tsp45I GTSAC 3 cut(s) 37, 133, 183
TspDTI ATGAA 5 cut(s) 144, 545, 546, 688, 813
TspGWI ACGGA 3 cut(s) 68, 89, 121
TspRI CASTG 3 cut(s) 142, 494, 678
VpaK11BI GGWCC 1 cut(s) 19
XceI RCATGY 1 cut(s) 637
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 1 cut(s) 189
ZrmI AGTACT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.