AT4G14695

Mediates the uptake of pyruvate into mitochondria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005252
Physical Location & Seq
Forward (+)
8419570 .. 8421070
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14695.2

Sequence Viewer

Length: 330 bp
ATGGCGACGTCTAAGCTTCAAGCTCTCTGGAATCACCCGGCAGGACCAAAGACAATTCATTTCTGGGCTCCAACATTTAAGTGGGGTATAAGCATTGCTAACATTGCAGATTTCCAGAAACCTCCAGAAAATATTTCGTACCTTCAGCAAATTGCTGTGACATGCACTGGAATGATTTGGTGTCGTTGCAGCACTATAATTACTCCTAAAAACTGGAACCTGTTTAGTGTTAATGTTGCTATGGCAGCTACAGGGATATACCAACTTACTCGCAAAATAAAGTATGATTATGTATCTGAAGCAGAGGCTGCTGTTGAAATTGAAGGATGA

Protein Analysis

109

Amino Acids

12.22

Weight (kDa)

8.49

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MPC PF03650 19 - 86 1.3e-20 Mitochondrial pyruvate carriers
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 11
AcuI CTGAAG 2 cut(s) 128, 318
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 1 cut(s) 140
AgsI TTSAA 3 cut(s) 20, 317, 323
AluBI AGCT 3 cut(s) 16, 23, 248
AluI AGCT 3 cut(s) 16, 23, 248
AlwNI CAGNNNCTG 1 cut(s) 308
ApeKI GCWGC 3 cut(s) 189, 245, 308
AspS9I GGNCC 1 cut(s) 44
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 70
BbvI GCAGC 3 cut(s) 201, 257, 295
BcnI CCSGG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 249
BisI GCNGC 3 cut(s) 190, 246, 309
BlsI GCNGC 3 cut(s) 191, 247, 310
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 69, 218
BmrFI CCNGG 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 108
BpuMI CCSGG 1 cut(s) 38
BsaHI GRCGYC 1 cut(s) 8
Bse1I ACTGG 2 cut(s) 172, 218
Bse3DI GCAATG 2 cut(s) 93, 102
BseMI GCAATG 2 cut(s) 93, 102
BseNI ACTGG 2 cut(s) 172, 218
BseXI GCAGC 3 cut(s) 201, 257, 295
BsiSI CCGG 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 70
BspLI GGNNCC 2 cut(s) 69, 218
BsrDI GCAATG 2 cut(s) 93, 102
BsrI ACTGG 2 cut(s) 172, 218
BssNI GRCGYC 1 cut(s) 8
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 104, 245, 308
BstNSI RCATGY 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 249
BstV1I GCAGC 3 cut(s) 201, 257, 295
BtsIMutI CAGTG 1 cut(s) 165
CaiI CAGNNNCTG 1 cut(s) 308
Cfr13I GGNCC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 139
CviAII CATG 1 cut(s) 162
CviJI RGCY 5 cut(s) 16, 23, 68, 248, 308
CviKI_1 RGCY 5 cut(s) 16, 23, 68, 248, 308
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 128, 318
EcoT38I GRGCYC 1 cut(s) 70
FaeI CATG 1 cut(s) 165
FaiI YATR 7 cut(s) 89, 163, 197, 242, 259, 285, 291
FatI CATG 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 190, 246, 309
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 3 cut(s) 190, 246, 309
GluI GCNGC 3 cut(s) 190, 246, 309
GsuI CTGGAG 1 cut(s) 108
HapII CCGG 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 1 cut(s) 31
HpaII CCGG 1 cut(s) 38
HphI GGTGA 1 cut(s) 26
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 3 cut(s) 28, 115, 125
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 152, 317
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 3 cut(s) 107, 165, 189
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 245, 308
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 1 cut(s) 8
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 1 cut(s) 165
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 3 cut(s) 201, 257, 295
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 4 cut(s) 54, 150, 198, 318
MmeI TCCRAC 1 cut(s) 95
MnlI CCTC 2 cut(s) 132, 298
MseI TTAA 2 cut(s) 78, 231
MslI CAYNNNNRTG 2 cut(s) 79, 170
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 104, 245, 308
NciI CCSGG 1 cut(s) 38
NlaIII CATG 1 cut(s) 165
NlaIV GGNNCC 2 cut(s) 69, 218
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 165
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 3 cut(s) 191, 247, 310
PspN4I GGNNCC 2 cut(s) 69, 218
PspPI GGNCC 1 cut(s) 44
PstNI CAGNNNCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 2 cut(s) 79, 170
SaqAI TTAA 2 cut(s) 78, 231
SatI GCNGC 3 cut(s) 190, 246, 309
Sau96I GGNCC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 70
SetI ASST 7 cut(s) 11, 18, 25, 124, 144, 222, 250
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 1 cut(s) 44
SmiMI CAYNNNNRTG 2 cut(s) 79, 170
Sse9I AATT 4 cut(s) 54, 150, 198, 318
SspI AATATT 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 36
TaiI ACGT 1 cut(s) 11
TasI AATT 4 cut(s) 54, 150, 198, 318
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 2 cut(s) 78, 231
Tru9I TTAA 2 cut(s) 78, 231
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 189, 245, 308
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 47
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 44
XceI RCATGY 1 cut(s) 165
XcmI CCANNNNNNNNNTGG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.