MD03G1193700.v1.1

Mitochondrial pyruvate carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005252
Physical Location & Seq
Reverse (-)
26602340 .. 26605125
2786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1193700.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGCAGCTTCCAAGGTCCAAGCTTTCTGGAACCACCCCGCAGGCCCTAAAACAATTCACTTCTGGGCTCCAACTTTTAAATGGGGTATTAGCATAGCAAATATTGCTGATTTCTCAAAGCCACCGGAGACGCTGTCTTATCCTCAGCAACTAGCTGTTACATGCACTGGACTTATTTGGTCTCGTTACAGTATGGTGATTACTCCGAAAAACTGGAATCTGTTTAGTGTCAATGTTGCAATGGCTGGGACAGGCTTGTATCAACTTTCCCGCAAAATCACGCACGACTACTTTTCCGACACTGAAGCAGCTGTAGCAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPC

Protein Analysis

109

Amino Acids

12.07

Weight (kDa)

7.9

Isoelectric Point (pI)

28.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MPC PF03650 6 - 104 6e-42 Mitochondrial pyruvate carriers
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 271
AcuI CTGAAG 1 cut(s) 324
AluBI AGCT 4 cut(s) 8, 23, 155, 311
AluI AGCT 4 cut(s) 8, 23, 155, 311
Alw26I GTCTC 2 cut(s) 122, 186
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 2 cut(s) 5, 308
AspS9I GGNCC 2 cut(s) 16, 44
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 70
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 2 cut(s) 17, 320
BcoDI GTCTC 2 cut(s) 122, 186
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 2 cut(s) 6, 309
BlsI GCNGC 2 cut(s) 7, 310
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 44
BmiI GGNNCC 2 cut(s) 32, 69
Bpu10I CCTNAGC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 124
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bse1I ACTGG 2 cut(s) 172, 218
Bse3DI GCAATG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 12
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 2 cut(s) 172, 218
BseXI GCAGC 2 cut(s) 17, 320
BseYI CCCAGC 1 cut(s) 245
BshFI GGCC 1 cut(s) 45
BsiSI CCGG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 262
BsmAI GTCTC 2 cut(s) 122, 186
BsmBI CGTCTC 1 cut(s) 122
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 1 cut(s) 45
Bso31I GGTCTC 1 cut(s) 186
Bsp1286I GDGCHC 1 cut(s) 70
BspACI CCGC 2 cut(s) 39, 271
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 32, 69
BspTNI GGTCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 246
BsrI ACTGG 2 cut(s) 172, 218
BssECI CCNNGG 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 191
Bst6I CTCTTC 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 144
BstMAI GTCTC 2 cut(s) 122, 186
BstMWI GCNNNNNNNGC 2 cut(s) 104, 314
BstNSI RCATGY 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 2 cut(s) 17, 320
BsuRI GGCC 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 165, 300
Cac8I GCNNGC 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 16, 44
CseI GACGC 1 cut(s) 139
CviAII CATG 1 cut(s) 162
CviJI RGCY 9 cut(s) 8, 23, 45, 68, 121, 155, 245, 255, 311
CviKI_1 RGCY 9 cut(s) 8, 23, 45, 68, 121, 155, 245, 255, 311
DdeI CTNAG 1 cut(s) 144
DraI TTTAAA 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 16
Eco57I CTGAAG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 44
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 12
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 1 cut(s) 165
FaiI YATR 3 cut(s) 95, 163, 194
FaqI GGGAC 1 cut(s) 262
FatI CATG 1 cut(s) 161
FauI CCCGC 2 cut(s) 46, 278
Fnu4HI GCNGC 2 cut(s) 6, 309
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 2 cut(s) 6, 309
FspBI CTAG 1 cut(s) 152
GluI GCNGC 2 cut(s) 6, 309
GsaI CCCAGC 1 cut(s) 249
HaeIII GGCC 1 cut(s) 45
HapII CCGG 1 cut(s) 125
HgaI GACGC 1 cut(s) 139
Hin1II CATG 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 1 cut(s) 217
HpaII CCGG 1 cut(s) 125
HphI GGTGA 1 cut(s) 208
Hpy188I TCNGA 2 cut(s) 207, 298
Hpy188III TCNNGA 1 cut(s) 28
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4V TGCA 2 cut(s) 165, 239
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 314
HpyF3I CTNAG 1 cut(s) 144
Hsp92II CATG 1 cut(s) 165
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 8 cut(s) 13, 27, 49, 138, 153, 199, 231, 237
Lsp1109I GCAGC 2 cut(s) 17, 320
MaeI CTAG 1 cut(s) 152
MaeIII GTNAC 2 cut(s) 157, 185
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 95, 321
MnlI CCTC 1 cut(s) 153
MseI TTAA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 104, 314
NlaIII CATG 1 cut(s) 165
NlaIV GGNNCC 2 cut(s) 32, 69
NspI RCATGY 1 cut(s) 165
PfeI GAWTC 1 cut(s) 217
PflFI GACNNNGTC 1 cut(s) 133
PkrI GCNGC 2 cut(s) 7, 310
PspFI CCCAGC 1 cut(s) 245
PspN4I GGNNCC 2 cut(s) 32, 69
PspPI GGNCC 2 cut(s) 16, 44
PsyI GACNNNGTC 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 311
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 2 cut(s) 6, 309
Sau96I GGNCC 2 cut(s) 16, 44
SduI GDGCHC 1 cut(s) 70
SetI ASST 5 cut(s) 10, 18, 25, 157, 313
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 16
Sse9I AATT 1 cut(s) 54
SsiI CCGC 2 cut(s) 39, 271
SspI AATATT 1 cut(s) 103
SspMI CTAG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 191
TasI AATT 1 cut(s) 54
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 2 cut(s) 172, 307
TseI GCWGC 2 cut(s) 5, 308
TspRI CASTG 2 cut(s) 172, 307
Tth111I GACNNNGTC 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 16
XceI RCATGY 1 cut(s) 165
XcmI CCANNNNNNNNNTGG 1 cut(s) 78
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.