AT4G17620

RAI1 like PD-(D/E)XK nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008690
Physical Location & Seq
Forward (+)
9821874 .. 9825039
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G17620.1

Sequence Viewer

Length: 1635 bp
ATGGATTCACCGCCGAAGAAAAATCCTATGGATGATTTGTTCGGCGAAGACTCCGACAACGACTCACGATCTTCGCGTTCGAAATCGTCTTCTTCCGGTAATGCTTCTTCGTCTTCTTCCTCCTCCTCGTCTTCTTCGTCTTCGTCTTCTGCAGCGGGAGGAGATGGAGAGGGAGATGGAGGTGGTGCGGATTCTGGCAGCGCAAGCGATAGCGGAAGTGGAAGTAGCGGCGGGAAAGAGGAGCATGGAGATGATAAAGTAGAGAGCTATAGAAGCAATGATAATGGAGAAAGTGGAGTTTATCCTTATGAAGAGGAGGAAGAAGAAGAAGAAGATGAGAAAGATCTTTTTGGGTCTGATAATGAAGAGTATACTAAGACACCTGCAATTAGCACTTACTCAATTCCAGTTTTGCCTGCGGGATGGAGCAATGATAACCATGGTGGGAGAGGGGGAATGGGACGTGGTCGTTGGTCAAATGGGAGAGGAGGACCTGGGCTTCTTCCTCGGCCTGGACCTTACCCTGGACGTGGTGGTAATCGTGGTGGTTTTGGCGGGAGATACCAGAATTATCAGCGTGATGAACGTTTTGTTTCTGAGCTTAAGCTTTCGAAAAGCAAAGAAACGTTGGCTAGAAAACAAACCAACTTTCAGGAGCCATGTGAGCTTACTTGCTACAGTCGAGTAGAAGGTGGAGAAGTAATCTACGATGACCAAGGATTGAGGCTTTTCAAACGTCATATTGGTGAAGAGATTGGAGCGGATTTAAATCAAGGTTATGATACTTTTATTGAGAAAAAAGATCTGGGCTCTGAGGGCTTTGGTGACCTTCTTGGCAGTATTAGAGCAAAAAACATTTCGCTGGACAACATTCATTTTGTGACTTTTCGTAATAATCTAAACAAGATATTGGGCGCTGCTTACAATCGGCATGAACCGTGGGAAATGGGCGTCCATAAGAGAAATGGAACCATATATCTAGATGTGCATAAACTGCCTGAAAGGCCGCAGAGTGACTTAGATCGCCGCAGATGTTACTGGGGTTATTGTTTTGAAAGCCTTGCTACGGAAGATCCAGGAAGAGCTTATGGAGAAGAAATACATCATGTGGATGCAAATGTTGAATTTTGTTCTGTTGTCAGGACTAAACTAGGAGCTCACCGAGTTATGATGGGTGCTGAAATGGACTGCTGTGATGTATCTGATAAGGGAAAAAGGTTTTACGTAGAGTTGAAAACAACTCGTGAGTTGGATGATCGTACCGTTGACAGATTTGAAAGAGAGAAATTGCTTAAATTCTGGATACAATCATTCGTAGCAGGAGTTCCATACATTGTCGTTGGATTTAGAGATGATGGAGGAAGACTAGTACGCACAGAGAGACTAACAACGAGAGATATTGCGCATAGAGCCAGATTGAAAAACTATTGGCAGGGAGGAGTGTGCTTGGCATTTGCCGATGAAGTTCTTTGCTGGCTATACGGCACTGTTAAAGAAAACGAAGATTACATACTTCAGTTCGTGCACCCTTTCATGAGACTAGAGCTTCTTCAAGCTCAATCTTGTCCTGATGCCATCACTAATCATGTTCACCTTCTACAAAATCCTGCATCACCACCACCACCACCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAI1

Protein Analysis

544

Amino Acids

60.38

Weight (kDa)

5.23

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAI1 PF08652 218 - 520 3.3e-84 RAI1 like PD-(D/E)XK nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc16I TGCGCA 1 cut(s) 1404
Acc36I ACCTGC 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 761
AccI GTMKAC 1 cut(s) 371
AccII CGCG 1 cut(s) 76
AclI AACGTT 2 cut(s) 586, 626
AclWI GGATC 1 cut(s) 1067
AcsI RAATTY 2 cut(s) 1124, 1295
AcuI CTGAAG 1 cut(s) 1499
AcyI GRCGYC 1 cut(s) 951
AfaI GTAC 2 cut(s) 1261, 1371
AfiI CCNNNNNNNGG 4 cut(s) 512, 524, 530, 1066
AflII CTTAAG 1 cut(s) 602
AgsI TTSAA 7 cut(s) 733, 1055, 1124, 1234, 1277, 1420, 1553
AhlI ACTAGT 1 cut(s) 1366
AjiI CACGTC 2 cut(s) 464, 530
AjnI CCWGG 4 cut(s) 493, 511, 523, 1075
AloI GAACNNNNNNTCC 2 cut(s) 23, 55
AluBI AGCT 8 cut(s) 267, 601, 607, 667, 1085, 1157, 1546, 1556
AluI AGCT 8 cut(s) 267, 601, 607, 667, 1085, 1157, 1546, 1556
Alw21I GWGCWC 2 cut(s) 1159, 1527
Alw26I GTCTC 2 cut(s) 1375, 1531
Alw44I GTGCAC 1 cut(s) 1523
AlwI GGATC 1 cut(s) 1067
AoxI GGCC 2 cut(s) 509, 1004
ApaLI GTGCAC 1 cut(s) 1523
ApeKI GCWGC 3 cut(s) 152, 198, 917
ApoI RAATTY 2 cut(s) 1124, 1295
AspLEI GCGC 3 cut(s) 203, 917, 1405
AspS9I GGNCC 2 cut(s) 491, 515
AsuHPI GGTGA 5 cut(s) 758, 836, 1151, 1583, 1605
AsuII TTCGAA 2 cut(s) 80, 611
AvaII GGWCC 2 cut(s) 491, 515
BaeGI GKGCMC 1 cut(s) 1527
BanII GRGCYC 2 cut(s) 812, 1159
BarI GAAGNNNNNNTAC 4 cut(s) 91, 123, 1494, 1526
BauI CACGAG 1 cut(s) 1242
BbsI GAAGAC 7 cut(s) 54, 81, 105, 123, 132, 138, 1369
Bbv12I GWGCWC 2 cut(s) 1159, 1527
BbvI GCAGC 3 cut(s) 164, 210, 904
BccI CCATC 6 cut(s) 158, 170, 417, 1165, 1349, 1583
BceAI ACGGC 1 cut(s) 1498
BciT130I CCWGG 4 cut(s) 495, 513, 525, 1077
BciVI GTATCC 1 cut(s) 1296
BcoDI GTCTC 2 cut(s) 1375, 1531
BcuI ACTAGT 1 cut(s) 1366
BfaI CTAG 5 cut(s) 633, 980, 1151, 1367, 1541
BfmI CTRYAG 3 cut(s) 150, 268, 676
BfoI RGCGCY 1 cut(s) 918
BfrI CTTAAG 1 cut(s) 602
BfuAI ACCTGC 1 cut(s) 391
BfuI GTATCC 1 cut(s) 1296
BglI GCCNNNNNGGC 1 cut(s) 1003
BglII AGATCT 2 cut(s) 343, 802
BisI GCNGC 6 cut(s) 153, 199, 229, 918, 1007, 1027
BlsI GCNGC 6 cut(s) 154, 200, 230, 919, 1008, 1028
Bme1390I CCNGG 4 cut(s) 495, 513, 525, 1077
Bme18I GGWCC 2 cut(s) 491, 515
BmgBI CACGTC 2 cut(s) 464, 530
BmgT120I GGNCC 2 cut(s) 491, 515
BmiI GGNNCC 2 cut(s) 657, 970
BmrFI CCNGG 4 cut(s) 495, 513, 525, 1077
BmrI ACTGGG 1 cut(s) 1048
BmsI GCATC 3 cut(s) 1102, 1561, 1619
BmuI ACTGGG 1 cut(s) 1048
BpiI GAAGAC 7 cut(s) 54, 81, 105, 123, 132, 138, 1369
Bpu14I TTCGAA 2 cut(s) 80, 611
BsaAI YACGTR 1 cut(s) 1225
BsaBI GATNNNNATC 1 cut(s) 768
BsaHI GRCGYC 1 cut(s) 951
BsaJI CCNNGG 6 cut(s) 439, 494, 506, 523, 715, 938
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 4 cut(s) 512, 524, 530, 1066
Bse1I ACTGG 2 cut(s) 407, 1043
Bse3DI GCAATG 2 cut(s) 283, 436
Bse8I GATNNNNATC 1 cut(s) 768
BseBI CCWGG 4 cut(s) 495, 513, 525, 1077
BseDI CCNNGG 6 cut(s) 439, 494, 506, 523, 715, 938
BseGI GGATG 4 cut(s) 37, 428, 1117, 1258
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 4 cut(s) 512, 524, 530, 1066
BseMI GCAATG 2 cut(s) 283, 436
BseMII CTCAG 2 cut(s) 588, 804
BseNI ACTGG 2 cut(s) 407, 1043
BseRI GAGGAG 7 cut(s) 112, 115, 174, 254, 329, 501, 1452
BseSI GKGCMC 1 cut(s) 1527
BseXI GCAGC 3 cut(s) 164, 210, 904
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 2 cut(s) 511, 1006
BsiHKAI GWGCWC 2 cut(s) 1159, 1527
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 4 cut(s) 512, 524, 530, 1066
BsmAI GTCTC 2 cut(s) 1375, 1531
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 2 cut(s) 511, 1006
Bsp119I TTCGAA 2 cut(s) 80, 611
Bsp1286I GDGCHC 3 cut(s) 812, 1159, 1527
Bsp143I GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
Bsp19I CCATGG 1 cut(s) 439
BspANI GGCC 2 cut(s) 511, 1006
BspCNI CTCAG 2 cut(s) 589, 805
BspFNI CGCG 1 cut(s) 76
BspHI TCATGA 1 cut(s) 1533
BspLI GGNNCC 2 cut(s) 657, 970
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 1 cut(s) 1067
BspQI GCTCTTC 1 cut(s) 1075
BspT104I TTCGAA 2 cut(s) 80, 611
BspTI CTTAAG 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 761
BsrDI GCAATG 2 cut(s) 283, 436
BsrI ACTGG 2 cut(s) 407, 1043
BssECI CCNNGG 6 cut(s) 439, 494, 506, 523, 715, 938
BssMI GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
BssNAI GTATAC 1 cut(s) 372
BssNI GRCGYC 1 cut(s) 951
BssSI CACGAG 1 cut(s) 1242
BssT1I CCWWGG 2 cut(s) 439, 715
Bst1107I GTATAC 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 1242
Bst2UI CCWGG 4 cut(s) 495, 513, 525, 1077
Bst4CI ACNGT 4 cut(s) 680, 939, 1264, 1489
Bst6I CTCTTC 4 cut(s) 306, 360, 744, 1075
BstACI GRCGYC 1 cut(s) 951
BstAFI CTTAAG 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 994
BstBAI YACGTR 1 cut(s) 1225
BstBI TTCGAA 2 cut(s) 80, 611
BstC8I GCNNGC 3 cut(s) 205, 417, 1475
BstDEI CTNAG 5 cut(s) 375, 597, 813, 1018, 1629
BstDSI CCRYGG 2 cut(s) 439, 938
BstEII GGTNACC 1 cut(s) 824
BstF5I GGATG 4 cut(s) 37, 428, 1117, 1258
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 918
BstHHI GCGC 3 cut(s) 203, 917, 1405
BstKTI GATC 6 cut(s) 71, 346, 805, 1024, 1075, 1258
BstMAI GTCTC 2 cut(s) 1375, 1531
BstMBI GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
BstMWI GCNNNNNNNGC 7 cut(s) 204, 273, 664, 816, 994, 1003, 1409
BstNI CCWGG 4 cut(s) 495, 513, 525, 1077
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 4 cut(s) 493, 511, 523, 1075
BstSFI CTRYAG 3 cut(s) 150, 268, 676
BstSLI GKGCMC 1 cut(s) 1527
BstSNI TACGTA 1 cut(s) 1225
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 3 cut(s) 164, 210, 904
BstV2I GAAGAC 7 cut(s) 54, 81, 105, 123, 132, 138, 1369
BstX2I RGATCY 3 cut(s) 343, 802, 1072
BstYI RGATCY 3 cut(s) 343, 802, 1072
BstZ17I GTATAC 1 cut(s) 372
BsuI GTATCC 1 cut(s) 1296
BsuRI GGCC 2 cut(s) 511, 1006
BtgI CCRYGG 2 cut(s) 439, 938
BtrI CACGTC 2 cut(s) 464, 530
BtsCI GGATG 4 cut(s) 37, 428, 1117, 1258
BtsIMutI CAGTG 1 cut(s) 1485
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 3 cut(s) 205, 417, 1475
CciI TCATGA 1 cut(s) 1533
CfoI GCGC 3 cut(s) 203, 917, 1405
Cfr13I GGNCC 2 cut(s) 491, 515
CseI GACGC 1 cut(s) 940
Csp6I GTAC 2 cut(s) 1260, 1370
CviAII CATG 7 cut(s) 245, 440, 660, 932, 1106, 1534, 1586
CviQI GTAC 2 cut(s) 1260, 1370
DdeI CTNAG 5 cut(s) 375, 597, 813, 1018, 1629
DpnI GATC 6 cut(s) 70, 345, 804, 1023, 1074, 1257
DpnII GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
DraI TTTAAA 1 cut(s) 768
Eam1104I CTCTTC 4 cut(s) 306, 360, 744, 1075
EarI CTCTTC 4 cut(s) 306, 360, 744, 1075
Ecl136II GAGCTC 1 cut(s) 1157
Eco105I TACGTA 1 cut(s) 1225
Eco130I CCWWGG 2 cut(s) 439, 715
Eco24I GRGCYC 2 cut(s) 812, 1159
Eco47I GGWCC 2 cut(s) 491, 515
Eco53kI GAGCTC 1 cut(s) 1157
Eco57I CTGAAG 1 cut(s) 1499
Eco91I GGTNACC 1 cut(s) 824
EcoICRI GAGCTC 1 cut(s) 1157
EcoO109I RGGNCCY 1 cut(s) 491
EcoO65I GGTNACC 1 cut(s) 824
EcoRII CCWGG 4 cut(s) 493, 511, 523, 1075
EcoT14I CCWWGG 2 cut(s) 439, 715
EcoT38I GRGCYC 2 cut(s) 812, 1159
ErhI CCWWGG 2 cut(s) 439, 715
FaeI CATG 7 cut(s) 248, 443, 663, 935, 1109, 1537, 1589
FaqI GGGAC 1 cut(s) 474
FatI CATG 7 cut(s) 244, 439, 659, 931, 1105, 1533, 1585
FauI CCCGC 4 cut(s) 148, 224, 412, 548
FblI GTMKAC 1 cut(s) 371
Fnu4HI GCNGC 6 cut(s) 153, 199, 229, 918, 1007, 1027
FokI GGATG 4 cut(s) 44, 435, 1124, 1265
FriOI GRGCYC 2 cut(s) 812, 1159
Fsp4HI GCNGC 6 cut(s) 153, 199, 229, 918, 1007, 1027
FspBI CTAG 5 cut(s) 633, 980, 1151, 1367, 1541
FspI TGCGCA 1 cut(s) 1404
GlaI GCGC 3 cut(s) 202, 916, 1404
GluI GCNGC 6 cut(s) 153, 199, 229, 918, 1007, 1027
HaeII RGCGCY 1 cut(s) 918
HaeIII GGCC 2 cut(s) 511, 1006
HapII CCGG 1 cut(s) 96
HgaI GACGC 1 cut(s) 940
HhaI GCGC 3 cut(s) 203, 917, 1405
Hin1I GRCGYC 1 cut(s) 951
Hin1II CATG 7 cut(s) 248, 443, 663, 935, 1109, 1537, 1589
Hin6I GCGC 3 cut(s) 201, 915, 1403
HinP1I GCGC 3 cut(s) 201, 915, 1403
HincII GTYRAC 1 cut(s) 1267
HindII GTYRAC 1 cut(s) 1267
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 4 cut(s) 5, 50, 62, 191
HpaII CCGG 1 cut(s) 96
HphI GGTGA 5 cut(s) 758, 836, 1151, 1583, 1605
Hpy166II GTNNAC 4 cut(s) 372, 1267, 1525, 1591
Hpy188I TCNGA 5 cut(s) 55, 358, 598, 814, 1204
Hpy188III TCNNGA 8 cut(s) 66, 653, 980, 1141, 1244, 1300, 1534, 1568
Hpy8I GTNNAC 4 cut(s) 372, 1267, 1525, 1591
HpyAV CCTTC 3 cut(s) 683, 839, 1604
HpyCH4III ACNGT 4 cut(s) 680, 939, 1264, 1489
HpyCH4IV ACGT 6 cut(s) 463, 529, 586, 626, 736, 1224
HpyCH4V TGCA 6 cut(s) 152, 386, 988, 1115, 1525, 1610
HpyF10VI GCNNNNNNNGC 7 cut(s) 204, 273, 664, 816, 994, 1003, 1409
HpyF3I CTNAG 5 cut(s) 375, 597, 813, 1018, 1629
HpySE526I ACGT 6 cut(s) 463, 529, 586, 626, 736, 1224
Hsp92I GRCGYC 1 cut(s) 951
Hsp92II CATG 7 cut(s) 248, 443, 663, 935, 1109, 1537, 1589
HspAI GCGC 3 cut(s) 201, 915, 1403
Kzo9I GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
LguI GCTCTTC 1 cut(s) 1075
LmnI GCTCC 5 cut(s) 241, 426, 655, 758, 1154
Lsp1109I GCAGC 3 cut(s) 164, 210, 904
LweI GCATC 3 cut(s) 1102, 1561, 1619
MaeI CTAG 5 cut(s) 633, 980, 1151, 1367, 1541
MaeII ACGT 6 cut(s) 463, 529, 586, 626, 736, 1224
MaeIII GTNAC 4 cut(s) 824, 880, 1013, 1034
MalI GATC 6 cut(s) 70, 345, 804, 1023, 1074, 1257
MbiI CCGCTC 1 cut(s) 761
MboI GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
MflI RGATCY 3 cut(s) 343, 802, 1072
MhlI GDGCHC 3 cut(s) 812, 1159, 1527
MluCI AATT 6 cut(s) 387, 402, 568, 1124, 1286, 1295
MlyI GAGTC 2 cut(s) 44, 56
MmeI TCCRAC 3 cut(s) 78, 1230, 1321
MseI TTAA 4 cut(s) 603, 767, 1293, 1491
MslI CAYNNNNRTG 3 cut(s) 249, 744, 1110
MspA1I CMGCKG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 4 cut(s) 495, 513, 525, 1077
MvaI CCWGG 4 cut(s) 495, 513, 525, 1077
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 7 cut(s) 204, 273, 664, 816, 994, 1003, 1409
NcoI CCATGG 1 cut(s) 439
NdeII GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
NlaIII CATG 7 cut(s) 248, 443, 663, 935, 1109, 1537, 1589
NlaIV GGNNCC 2 cut(s) 657, 970
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 3 cut(s) 824, 880, 1013
NsbI TGCGCA 1 cut(s) 1404
NspV TTCGAA 2 cut(s) 80, 611
PagI TCATGA 1 cut(s) 1533
PaqCI CACCTGC 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 1075
PcsI WCGNNNNNNNCGW 2 cut(s) 73, 134
PfeI GAWTC 2 cut(s) 5, 191
PflFI GACNNNGTC 1 cut(s) 465
PfoI TCCNGGA 1 cut(s) 1075
PkrI GCNGC 6 cut(s) 154, 200, 230, 919, 1008, 1028
PleI GAGTC 2 cut(s) 44, 56
PpsI GAGTC 2 cut(s) 44, 56
Ppu21I YACGTR 1 cut(s) 1225
PpuMI RGGWCCY 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 1159
Psp1406I AACGTT 2 cut(s) 586, 626
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 4 cut(s) 493, 511, 523, 1075
PspEI GGTNACC 1 cut(s) 824
PspGI CCWGG 4 cut(s) 493, 511, 523, 1075
PspN4I GGNNCC 2 cut(s) 657, 970
PspPI GGNCC 2 cut(s) 491, 515
PspPPI RGGWCCY 1 cut(s) 491
PsrI GAACNNNNNNTAC 2 cut(s) 1308, 1340
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 3 cut(s) 343, 802, 1072
PsyI GACNNNGTC 1 cut(s) 465
RsaI GTAC 2 cut(s) 1261, 1371
RsaNI GTAC 2 cut(s) 1260, 1370
RseI CAYNNNNRTG 3 cut(s) 249, 744, 1110
SacI GAGCTC 1 cut(s) 1159
SapI GCTCTTC 1 cut(s) 1075
SaqAI TTAA 4 cut(s) 603, 767, 1293, 1491
SatI GCNGC 6 cut(s) 153, 199, 229, 918, 1007, 1027
Sau3AI GATC 6 cut(s) 68, 343, 802, 1021, 1072, 1255
Sau96I GGNCC 2 cut(s) 491, 515
SchI GAGTC 2 cut(s) 44, 56
ScrFI CCNGG 4 cut(s) 495, 513, 525, 1077
SduI GDGCHC 3 cut(s) 812, 1159, 1527
SfaNI GCATC 3 cut(s) 1102, 1561, 1619
SfcI CTRYAG 3 cut(s) 150, 268, 676
SfuI TTCGAA 2 cut(s) 80, 611
SinI GGWCC 2 cut(s) 491, 515
SmiI ATTTAAAT 1 cut(s) 768
SmiMI CAYNNNNRTG 3 cut(s) 249, 744, 1110
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SnaBI TACGTA 1 cut(s) 1225
SpeI ACTAGT 1 cut(s) 1366
Sse9I AATT 6 cut(s) 387, 402, 568, 1124, 1286, 1295
SspMI CTAG 5 cut(s) 633, 980, 1151, 1367, 1541
SstI GAGCTC 1 cut(s) 1159
StyD4I CCNGG 4 cut(s) 493, 511, 523, 1075
StyI CCWWGG 2 cut(s) 439, 715
SwaI ATTTAAAT 1 cut(s) 768
TaaI ACNGT 4 cut(s) 680, 939, 1264, 1489
TaiI ACGT 6 cut(s) 466, 532, 589, 629, 739, 1227
TaqI TCGA 3 cut(s) 80, 611, 682
TasI AATT 6 cut(s) 387, 402, 568, 1124, 1286, 1295
TauI GCSGC 3 cut(s) 231, 1009, 1029
TfiI GAWTC 2 cut(s) 5, 191
Tru1I TTAA 4 cut(s) 603, 767, 1293, 1491
Tru9I TTAA 4 cut(s) 603, 767, 1293, 1491
TscAI CASTG 1 cut(s) 1492
TseFI GTSAC 3 cut(s) 824, 880, 1013
TseI GCWGC 3 cut(s) 152, 198, 917
Tsp45I GTSAC 3 cut(s) 824, 880, 1013
TspDTI ATGAA 7 cut(s) 324, 378, 597, 863, 948, 1476, 1522
TspGWI ACGGA 1 cut(s) 1082
TspRI CASTG 1 cut(s) 1492
Tth111I GACNNNGTC 1 cut(s) 465
Vha464I CTTAAG 1 cut(s) 602
VneI GTGCAC 1 cut(s) 1523
VpaK11BI GGWCC 2 cut(s) 491, 515
XapI RAATTY 2 cut(s) 1124, 1295
XbaI TCTAGA 1 cut(s) 979
XcmI CCANNNNNNNNNTGG 1 cut(s) 962
XmiI GTMKAC 1 cut(s) 371
XspI CTAG 5 cut(s) 633, 980, 1151, 1367, 1541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.