Rh7CG243500

RAI1 like PD-(D/E)XK nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008690
Physical Location & Seq
Reverse (-)
22777688 .. 22795238
17551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG243500.1

Sequence Viewer

Length: 1296 bp
ATGGATTTCGCAAAGCAGCACGCCGATATCTTCGGCGAGGACGAAGACGATGAACCTAACAACCAAGATGGGACCCATTCCTCCTCCCCCTCCTCATCCTCATCCTCCTCCTCCTCCTCTTCTTCCTCGTCGTCTGCTGCTTCGTCTTCCGGTAGCGAGAAAGGCGAGTCCAGCAGCGGCAATAGCCGAAGCGGCGCCACCACTGGCGGTGGTGGTGCTGGTGACGAAGAGGAGGAGAACGGTGAAGAGGCCACCACTAATAATGAGTACCACGATAGGGGCGATTTTGGGGACGACGATAAGGATCTGTTTGGCTCTGATAATGAAGACTACACTAGAACCCCTGCTACTAGCCCTTTCCTTATTCCTGTATTGCCTGTGGTACGGAATAATAATCATAGTCAGGGTAGAGGGAGTTTTGGGCGTGGTCGTTGGCAAGCGGGGCATCAAAATGACAGAGGTGCTGGCATCCTTCCCCGACCTGGACCTTATCCGCAGAGGGGGCATAATTACGGTTATGGAAAGTTCTCAAATGGTCGTCATGACGAACGTTTCGTTTCTGAACTTAGACTGTCAAAGAGTGAAGAAACGTTATCAAGAAAGGTTATTCAGATTCAGGAACCCTGTGAACTTGCTTGCTATAGTCGGGTAGAAGGTGGAGATGTCAACTTTGATGAGAGCAGCTTGAGGCTTTTCAAGCGGCTTATTACTGAAGACATTGGAGCCGATCTTAATCAAGGCTTTGATACTTTCGTAGCAAAGAAAGATTTAGGCTCTGAAGGTTTTGGTGACCTTCTTGCCTGCATCAGAGACAAGAATATTCCACTTCAAAATATTCATTTTGTGACTTTTCGCAACAATCTCAATAAGATATTGGCCACTGCTTATATTCGGCATGAGCCTTGGGAAATGGGAGTGCATAAAAGGAATGGGGTTGTCTATCTTGATGTACATAAACTTCCGGAAAGGCCACAAAATGAGATGGATCGTCGAAGGTGTTACTGGGGATACTGTTTTGAGAGTCTTGCAACTGAAGATCCAGGAAGAGGTGATGGAGAGTTGATACATCACGTCGATGCCAATATTGAATACTGTTCAGTGATCAAAACCAAATTAGGGGCTCATCGTATTCTGATGGGCGCTGAAATGGATTGCTGTGATTCAACTGATGATGGAAGGAGATTTTACGTGGAGTTAAAGACAAGTCGTGAGTTGGAGTACCATACTGAGGAAAGATATGAGAGAGAGAAGCTCCTCAAATTTTGGGTACAGTCAGTTCTTATGAAGACCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAI1

Protein Analysis

431

Amino Acids

48.17

Weight (kDa)

5.15

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAI1 PF08652 206 - 428 3.9e-59 RAI1 like PD-(D/E)XK nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccIII TCCGGA 1 cut(s) 961
AciI CCGC 6 cut(s) 177, 192, 207, 440, 494, 700
AclI AACGTT 2 cut(s) 550, 590
AclWI GGATC 3 cut(s) 312, 993, 1031
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 1 cut(s) 1259
AcuI CTGAAG 3 cut(s) 732, 798, 1053
AcyI GRCGYC 1 cut(s) 195
AfaI GTAC 5 cut(s) 269, 384, 951, 1220, 1269
AfiI CCNNNNNNNGG 6 cut(s) 277, 482, 500, 1046, 1116, 1228
AgsI TTSAA 4 cut(s) 697, 830, 1088, 1164
AjiI CACGTC 1 cut(s) 1072
AjnI CCWGG 2 cut(s) 481, 1039
AluBI AGCT 2 cut(s) 684, 1252
AluI AGCT 2 cut(s) 684, 1252
Alw26I GTCTC 1 cut(s) 804
AlwI GGATC 3 cut(s) 312, 993, 1031
Aor13HI TCCGGA 1 cut(s) 961
AoxI GGCC 3 cut(s) 249, 876, 968
ApeKI GCWGC 4 cut(s) 16, 137, 174, 681
ApoI RAATTY 1 cut(s) 1259
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AspLEI GCGC 2 cut(s) 197, 1142
AspS9I GGNCC 2 cut(s) 72, 485
AsuHPI GGTGA 4 cut(s) 233, 254, 800, 1061
AvaII GGWCC 2 cut(s) 72, 485
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BalI TGGCCA 1 cut(s) 878
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 1123
BarI GAAGNNNNNNTAC 2 cut(s) 942, 974
BbsI GAAGAC 5 cut(s) 51, 138, 333, 720, 1292
BbvI GCAGC 4 cut(s) 28, 124, 186, 693
BccI CCATC 5 cut(s) 62, 976, 1046, 1129, 1166
BciT130I CCWGG 2 cut(s) 483, 1041
BciVI GTATCC 1 cut(s) 1001
BclI TGATCA 1 cut(s) 1101
BcoDI GTCTC 1 cut(s) 804
BfaI CTAG 2 cut(s) 336, 351
BfmI CTRYAG 1 cut(s) 640
BfoI RGCGCY 2 cut(s) 198, 1143
BfuI GTATCC 1 cut(s) 1001
BglI GCCNNNNNGGC 1 cut(s) 192
BisI GCNGC 7 cut(s) 17, 138, 175, 178, 193, 682, 701
BlsI GCNGC 7 cut(s) 18, 139, 176, 179, 194, 683, 702
Bme1390I CCNGG 2 cut(s) 483, 1041
Bme18I GGWCC 2 cut(s) 72, 485
BmgBI CACGTC 1 cut(s) 1072
BmgT120I GGNCC 2 cut(s) 72, 485
BmiI GGNNCC 5 cut(s) 73, 74, 196, 621, 724
BmrFI CCNGG 2 cut(s) 483, 1041
BmrI ACTGGG 1 cut(s) 1012
BmsI GCATC 4 cut(s) 454, 477, 813, 1066
BmuI ACTGGG 1 cut(s) 1012
BpiI GAAGAC 5 cut(s) 51, 138, 333, 720, 1292
BplI GAGNNNNNCTC 2 cut(s) 1236, 1268
BpuEI CTTGAG 1 cut(s) 706
BsaAI YACGTR 1 cut(s) 1189
BsaBI GATNNNNATC 2 cut(s) 303, 732
BsaHI GRCGYC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 902
BsaWI WCCGGW 2 cut(s) 149, 961
Bsc4I CCNNNNNNNGG 6 cut(s) 277, 482, 500, 1046, 1116, 1228
Bse1I ACTGG 2 cut(s) 208, 1007
Bse8I GATNNNNATC 2 cut(s) 303, 732
BseAI TCCGGA 1 cut(s) 961
BseBI CCWGG 2 cut(s) 483, 1041
BseDI CCNNGG 1 cut(s) 902
BseGI GGATG 3 cut(s) 95, 101, 468
BseJI GATNNNNATC 2 cut(s) 303, 732
BseLI CCNNNNNNNGG 6 cut(s) 277, 482, 500, 1046, 1116, 1228
BseMII CTCAG 1 cut(s) 1218
BseNI ACTGG 2 cut(s) 208, 1007
BseRI GAGGAG 9 cut(s) 73, 82, 97, 100, 103, 106, 245, 248, 1244
BseXI GCAGC 4 cut(s) 28, 124, 186, 693
BshFI GGCC 3 cut(s) 251, 878, 970
BshNI GGYRCC 1 cut(s) 194
BsiSI CCGG 2 cut(s) 150, 962
BslFI GGGAC 2 cut(s) 85, 305
BslI CCNNNNNNNGG 6 cut(s) 277, 482, 500, 1046, 1116, 1228
BsmAI GTCTC 1 cut(s) 804
BsmFI GGGAC 2 cut(s) 85, 305
BsnI GGCC 3 cut(s) 251, 878, 970
Bsp1286I GDGCHC 1 cut(s) 1123
Bsp13I TCCGGA 1 cut(s) 961
Bsp1407I TGTACA 1 cut(s) 949
Bsp143I GATC 5 cut(s) 304, 727, 985, 1036, 1101
BspACI CCGC 6 cut(s) 177, 192, 207, 440, 494, 700
BspANI GGCC 3 cut(s) 251, 878, 970
BspCNI CTCAG 1 cut(s) 1219
BspEI TCCGGA 1 cut(s) 961
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 5 cut(s) 73, 74, 196, 621, 724
BspPI GGATC 3 cut(s) 312, 993, 1031
BspT107I GGYRCC 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 949
BsrI ACTGG 2 cut(s) 208, 1007
BssECI CCNNGG 1 cut(s) 902
BssMI GATC 5 cut(s) 304, 727, 985, 1036, 1101
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 902
Bst2UI CCWGG 2 cut(s) 483, 1041
Bst4CI ACNGT 6 cut(s) 242, 515, 573, 1013, 1094, 1272
Bst6I CTCTTC 4 cut(s) 124, 222, 240, 1039
BstACI GRCGYC 1 cut(s) 195
BstAUI TGTACA 1 cut(s) 949
BstBAI YACGTR 1 cut(s) 1189
BstC8I GCNNGC 5 cut(s) 21, 438, 466, 637, 802
BstDEI CTNAG 2 cut(s) 566, 1227
BstEII GGTNACC 1 cut(s) 788
BstF5I GGATG 3 cut(s) 95, 101, 468
BstH2I RGCGCY 2 cut(s) 198, 1143
BstHHI GCGC 2 cut(s) 197, 1142
BstKTI GATC 5 cut(s) 307, 730, 988, 1039, 1104
BstMAI GTCTC 1 cut(s) 804
BstMBI GATC 5 cut(s) 304, 727, 985, 1036, 1101
BstMWI GCNNNNNNNGC 7 cut(s) 162, 171, 183, 192, 442, 502, 697
BstNI CCWGG 2 cut(s) 483, 1041
BstPI GGTNACC 1 cut(s) 788
BstSCI CCNGG 2 cut(s) 481, 1039
BstSFI CTRYAG 1 cut(s) 640
BstV1I GCAGC 4 cut(s) 28, 124, 186, 693
BstV2I GAAGAC 5 cut(s) 51, 138, 333, 720, 1292
BstX2I RGATCY 2 cut(s) 304, 1036
BstYI RGATCY 2 cut(s) 304, 1036
BsuI GTATCC 1 cut(s) 1001
BsuRI GGCC 3 cut(s) 251, 878, 970
BtrI CACGTC 1 cut(s) 1072
BtsCI GGATG 3 cut(s) 95, 101, 468
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 3 cut(s) 201, 879, 1104
Cac8I GCNNGC 5 cut(s) 21, 438, 466, 637, 802
CciI TCATGA 1 cut(s) 541
CfoI GCGC 2 cut(s) 197, 1142
Cfr13I GGNCC 2 cut(s) 72, 485
Csp6I GTAC 5 cut(s) 268, 383, 950, 1219, 1268
CviAII CATG 2 cut(s) 542, 896
CviQI GTAC 5 cut(s) 268, 383, 950, 1219, 1268
DdeI CTNAG 2 cut(s) 566, 1227
DinI GGCGCC 1 cut(s) 196
DpnI GATC 5 cut(s) 306, 729, 987, 1038, 1103
DpnII GATC 5 cut(s) 304, 727, 985, 1036, 1101
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 4 cut(s) 124, 222, 240, 1039
EarI CTCTTC 4 cut(s) 124, 222, 240, 1039
Eco130I CCWWGG 1 cut(s) 902
Eco24I GRGCYC 1 cut(s) 1123
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 2 cut(s) 72, 485
Eco57I CTGAAG 3 cut(s) 732, 798, 1053
Eco91I GGTNACC 1 cut(s) 788
EcoO109I RGGNCCY 1 cut(s) 72
EcoO65I GGTNACC 1 cut(s) 788
EcoRII CCWGG 2 cut(s) 481, 1039
EcoRV GATATC 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 902
EcoT38I GRGCYC 1 cut(s) 1123
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 902
FaeI CATG 2 cut(s) 545, 899
FaqI GGGAC 2 cut(s) 85, 305
FatI CATG 2 cut(s) 541, 895
FauI CCCGC 1 cut(s) 433
FbaI TGATCA 1 cut(s) 1101
Fnu4HI GCNGC 7 cut(s) 17, 138, 175, 178, 193, 682, 701
FokI GGATG 3 cut(s) 82, 88, 455
FriOI GRGCYC 1 cut(s) 1123
Fsp4HI GCNGC 7 cut(s) 17, 138, 175, 178, 193, 682, 701
FspBI CTAG 2 cut(s) 336, 351
GlaI GCGC 2 cut(s) 196, 1141
GluI GCNGC 7 cut(s) 17, 138, 175, 178, 193, 682, 701
HaeII RGCGCY 2 cut(s) 198, 1143
HaeIII GGCC 3 cut(s) 251, 878, 970
HapII CCGG 2 cut(s) 150, 962
HhaI GCGC 2 cut(s) 197, 1142
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 2 cut(s) 545, 899
Hin6I GCGC 2 cut(s) 195, 1140
HinP1I GCGC 2 cut(s) 195, 1140
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 4 cut(s) 167, 613, 1021, 1160
HpaII CCGG 2 cut(s) 150, 962
HphI GGTGA 4 cut(s) 233, 254, 800, 1061
Hpy166II GTNNAC 2 cut(s) 629, 667
Hpy188I TCNGA 6 cut(s) 319, 562, 612, 778, 809, 1134
Hpy188III TCNNGA 6 cut(s) 542, 597, 617, 944, 962, 1208
Hpy8I GTNNAC 2 cut(s) 629, 667
Hpy99I CGWCG 4 cut(s) 133, 299, 993, 1076
HpyAV CCTTC 6 cut(s) 482, 647, 773, 803, 987, 1170
HpyCH4III ACNGT 6 cut(s) 242, 515, 573, 1013, 1094, 1272
HpyCH4IV ACGT 4 cut(s) 550, 590, 1071, 1188
HpyCH4V TGCA 3 cut(s) 804, 919, 1028
HpyF10VI GCNNNNNNNGC 7 cut(s) 162, 171, 183, 192, 442, 502, 697
HpyF3I CTNAG 2 cut(s) 566, 1227
HpySE526I ACGT 4 cut(s) 550, 590, 1071, 1188
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 2 cut(s) 545, 899
HspAI GCGC 2 cut(s) 195, 1140
KasI GGCGCC 1 cut(s) 194
KflI GGGWCCC 1 cut(s) 72
Kpn2I TCCGGA 1 cut(s) 961
Ksp22I TGATCA 1 cut(s) 1101
Kzo9I GATC 5 cut(s) 304, 727, 985, 1036, 1101
LmnI GCTCC 2 cut(s) 722, 1257
Lsp1109I GCAGC 4 cut(s) 28, 124, 186, 693
LweI GCATC 4 cut(s) 454, 477, 813, 1066
MaeI CTAG 2 cut(s) 336, 351
MaeII ACGT 4 cut(s) 550, 590, 1071, 1188
MaeIII GTNAC 4 cut(s) 221, 788, 844, 998
MalI GATC 5 cut(s) 306, 729, 987, 1038, 1103
MboI GATC 5 cut(s) 304, 727, 985, 1036, 1101
MflI RGATCY 2 cut(s) 304, 1036
MhlI GDGCHC 1 cut(s) 1123
MlsI TGGCCA 1 cut(s) 878
MluCI AATT 3 cut(s) 508, 1112, 1259
MluNI TGGCCA 1 cut(s) 878
Mly113I GGCGCC 1 cut(s) 195
MlyI GAGTC 2 cut(s) 176, 1030
MmeI TCCRAC 1 cut(s) 1194
Mox20I TGGCCA 1 cut(s) 878
MroI TCCGGA 1 cut(s) 961
MscI TGGCCA 1 cut(s) 878
MseI TTAA 2 cut(s) 732, 1196
MslI CAYNNNNRTG 2 cut(s) 450, 1074
Msp20I TGGCCA 1 cut(s) 878
MspA1I CMGCKG 1 cut(s) 177
MspI CCGG 2 cut(s) 150, 962
MspR9I CCNGG 2 cut(s) 483, 1041
MvaI CCWGG 2 cut(s) 483, 1041
MwoI GCNNNNNNNGC 7 cut(s) 162, 171, 183, 192, 442, 502, 697
NarI GGCGCC 1 cut(s) 195
NdeII GATC 5 cut(s) 304, 727, 985, 1036, 1101
NlaIII CATG 2 cut(s) 545, 899
NlaIV GGNNCC 5 cut(s) 73, 74, 196, 621, 724
NmuCI GTSAC 3 cut(s) 221, 788, 844
PagI TCATGA 1 cut(s) 541
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 279, 552
PfeI GAWTC 2 cut(s) 613, 1160
PfoI TCCNGGA 1 cut(s) 1039
PkrI GCNGC 7 cut(s) 18, 139, 176, 179, 194, 683, 702
PleI GAGTC 2 cut(s) 175, 1029
PluTI GGCGCC 1 cut(s) 198
PpsI GAGTC 2 cut(s) 175, 1029
Ppu21I YACGTR 1 cut(s) 1189
PpuMI RGGWCCY 1 cut(s) 72
Psp1406I AACGTT 2 cut(s) 550, 590
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 481, 1039
PspEI GGTNACC 1 cut(s) 788
PspGI CCWGG 2 cut(s) 481, 1039
PspN4I GGNNCC 5 cut(s) 73, 74, 196, 621, 724
PspPI GGNCC 2 cut(s) 72, 485
PspPPI RGGWCCY 1 cut(s) 72
PsrI GAACNNNNNNTAC 4 cut(s) 331, 363, 1260, 1292
PsuI RGATCY 2 cut(s) 304, 1036
RsaI GTAC 5 cut(s) 269, 384, 951, 1220, 1269
RsaNI GTAC 5 cut(s) 268, 383, 950, 1219, 1268
RseI CAYNNNNRTG 2 cut(s) 450, 1074
SaqAI TTAA 2 cut(s) 732, 1196
SatI GCNGC 7 cut(s) 17, 138, 175, 178, 193, 682, 701
Sau3AI GATC 5 cut(s) 304, 727, 985, 1036, 1101
Sau96I GGNCC 2 cut(s) 72, 485
SchI GAGTC 2 cut(s) 176, 1030
ScrFI CCNGG 2 cut(s) 483, 1041
SduI GDGCHC 1 cut(s) 1123
SfaNI GCATC 4 cut(s) 454, 477, 813, 1066
SfcI CTRYAG 1 cut(s) 640
SfoI GGCGCC 1 cut(s) 196
SinI GGWCC 2 cut(s) 72, 485
SmiMI CAYNNNNRTG 2 cut(s) 450, 1074
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
Sse9I AATT 3 cut(s) 508, 1112, 1259
SsiI CCGC 6 cut(s) 177, 192, 207, 440, 494, 700
SspDI GGCGCC 1 cut(s) 194
SspI AATATT 3 cut(s) 820, 835, 1084
SspMI CTAG 2 cut(s) 336, 351
StyD4I CCNGG 2 cut(s) 481, 1039
StyI CCWWGG 1 cut(s) 902
TaaI ACNGT 6 cut(s) 242, 515, 573, 1013, 1094, 1272
TaiI ACGT 4 cut(s) 553, 593, 1074, 1191
TaqI TCGA 2 cut(s) 991, 1074
TasI AATT 3 cut(s) 508, 1112, 1259
TatI WGTACW 1 cut(s) 949
TauI GCSGC 3 cut(s) 180, 195, 703
TfiI GAWTC 2 cut(s) 613, 1160
Tru1I TTAA 2 cut(s) 732, 1196
Tru9I TTAA 2 cut(s) 732, 1196
TscAI CASTG 3 cut(s) 208, 886, 1104
TseFI GTSAC 3 cut(s) 221, 788, 844
TseI GCWGC 4 cut(s) 16, 137, 174, 681
Tsp45I GTSAC 3 cut(s) 221, 788, 844
TspDTI ATGAA 3 cut(s) 66, 339, 827
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 3 cut(s) 208, 886, 1104
VpaK11BI GGWCC 2 cut(s) 72, 485
XapI RAATTY 1 cut(s) 1259
XspI CTAG 2 cut(s) 336, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.