MD00G1132300.v1.1

Decapping nuclease DXO homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008690
Physical Location & Seq
Forward (+)
28647123 .. 28653010
5888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1132300.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGATTTTTCAGAACACCACAAAAATATCTTCGGCCCAGACTACGATGACGACGATGATGGTAATAAACAAGATGGGGCCCACTCATCCTCACCGTCTTCCTCATCGTCGCCTTCGACGTCGTCGTCGTCGTCTTCGTCATCGTCGGCTTCTTCTTCATCCAACGAAGGAGGGGAGTCAAGCAGTGGGAATAGCCCGAGCGCAAGCAGTGGCGGTGGCGCCGGCGGTGGAGGTGAAGAAGAAGTAGAGAATGGCGAAGAGGCTGATTATTCAAACGCTGCCGAGGACGACAATGAGTTCAGCAATATGGGTTACTTTGGGGAGGAAGACAGGGATCTGTTTGGCTCTGACAATGAAGATTACTGTAAAACCCCTGCTACTAGTCCTTTCCCTATTCCTGTATTACCTGTCATACGCAGTACGAATAATCAGGGTAGAGGGAATCCAGGGCGTGGTCGTCGGCAATCTGGACGTCCAAATGATAGAGGCGCAGGCATCCTTGGTCGAGGCGGACCTTTTCAACAGAGGCAAAATTTCGGATATGGTGGCTCTAATGGTTATCGTGATGAACGTTTTATCTCGGAATTAAAACTACTTTCAAAGGGTGAAGAAACACTGTCAAGAAAGGCCGTTGCATTTCAGGAACCCTGTGAGCTTGCTTGCTATAGTCGGGTAGAAGGTGGAGATGTCAGCTTTGACGATACCAGCTTGAGGCTTTTCAAGCGTCTTATTACTGAAGAAATTGGAAGAGATCTTAATGAAGGTTATGATACTTTTATTGCGAAGAAAGATTTAGGCTCTCAAGGTTTTGGTGATCTTCTCGCCTGCATAAGAGACAAGAATATTCCGCTTCAAAACATTCATTTTGTGACTTATCGCAACAATCTAAATAAGATATTGGCCACTTTTTATAACCAAAGGGATCCTTGGGAGATGGGAGTACATAAAAGAAATGGGGTTGTCTATCTTGATGTGCATAAACTACCTGAAAGGCCACAGACTGAGTTGGATCGAAGAAGGTGTTACTGGGGGTATTGTTTTGAGAGTCTTGCCACTGAAGATCCAGGAAGAGGCGATGGACCGATACATCATGTTGATGCCAATGTTGAATACTGTTCTGTGATCAAAACAAAATTAGGGGCTCATCGTATTCTGATGGGTGCTGAAATGGATTGCTGTGATTCAACTGATGATGGAAGGAGGTTTTATGTGGAATTAAAGACAAGCCATGAGTTGGACAACCATAAGATAGAGTACGAGTTTGAGAAAGAAAAGCTCCTCAAATTTTGGATTCAATCATTCTTAGCGGGTGTTCCCTATATCGTCATTGGATTTAGGAACGATGCAGGTCAACTTGTCCGCACAGAGAGACTAAGAACCAAAGATATAACACACAGAGTGAAAATGAAGAACTACTGGCAGGGAGGAGTTTGCTTGGCATTTGCTGATGAGGTATTATGCTGGCTATATGGGACTGTAAAAGAGAATGAAGACTACATATTGCAGTTTACTCATCCTTTCCAACGTTTGGAGCTTTTACAAGCCCAATCTTGCCCAGAAGAAATCACTAACCATGTTCAGCAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAI1

Protein Analysis

530

Amino Acids

59.12

Weight (kDa)

5.05

Isoelectric Point (pI)

57.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAI1 PF08652 214 - 516 2e-85 RAI1 like PD-(D/E)XK nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 912
AasI GACNNNNNNGTC 1 cut(s) 124
AatII GACGTC 2 cut(s) 122, 475
Acc36I ACCTGC 1 cut(s) 1337
AccB1I GGYRCC 1 cut(s) 218
AccB7I CCANNNNNTGG 3 cut(s) 452, 1234, 1528
AciI CCGC 6 cut(s) 213, 225, 510, 848, 1307, 1360
AclI AACGTT 2 cut(s) 571, 1525
AclWI GGATC 5 cut(s) 342, 917, 930, 1017, 1055
AcoI YGGCCR 1 cut(s) 900
AcsI RAATTY 2 cut(s) 532, 1283
AcuI CTGAAG 2 cut(s) 756, 1077
AcyI GRCGYC 3 cut(s) 119, 219, 472
AdeI CACNNNGTG 1 cut(s) 1399
AfaI GTAC 3 cut(s) 421, 942, 1256
AfiI CCNNNNNNNGG 5 cut(s) 452, 711, 1070, 1234, 1528
AgsI TTSAA 8 cut(s) 273, 521, 600, 721, 854, 1109, 1185, 1295
AhlI ACTAGT 1 cut(s) 380
AjnI CCWGG 2 cut(s) 445, 1063
AluBI AGCT 5 cut(s) 655, 693, 708, 1276, 1534
AluI AGCT 5 cut(s) 655, 693, 708, 1276, 1534
Alw26I GTCTC 2 cut(s) 828, 1363
AlwI GGATC 5 cut(s) 342, 917, 930, 1017, 1055
AlwNI CAGNNNCTG 1 cut(s) 1585
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 5 cut(s) 34, 78, 627, 900, 992
ApaI GGGCCC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 2 cut(s) 532, 1283
AspLEI GCGC 3 cut(s) 203, 221, 491
AspS9I GGNCC 5 cut(s) 35, 78, 79, 512, 1079
AsuHPI GGTGA 4 cut(s) 84, 245, 617, 824
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 2 cut(s) 512, 1079
BaeGI GKGCMC 1 cut(s) 82
BalI TGGCCA 1 cut(s) 902
BamHI GGATCC 1 cut(s) 922
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 2 cut(s) 82, 1144
BbsI GAAGAC 4 cut(s) 90, 126, 333, 1497
BbvI GCAGC 1 cut(s) 265
BccI CCATC 6 cut(s) 53, 68, 928, 1070, 1150, 1187
BceAI ACGGC 1 cut(s) 614
BciT130I CCWGG 2 cut(s) 447, 1065
BclI TGATCA 1 cut(s) 1122
BcoDI GTCTC 2 cut(s) 828, 1363
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 2 cut(s) 664, 1586
BfoI RGCGCY 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 1337
BglII AGATCT 1 cut(s) 751
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 447, 1065
Bme18I GGWCC 2 cut(s) 512, 1079
BmeT110I CYCGRG 1 cut(s) 196
BmgT120I GGNCC 5 cut(s) 35, 78, 79, 512, 1079
BmiI GGNNCC 5 cut(s) 79, 80, 220, 645, 924
BmrFI CCNGG 2 cut(s) 447, 1065
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 3 cut(s) 504, 1087, 1333
BmuI ACTGGG 1 cut(s) 1036
BpiI GAAGAC 4 cut(s) 90, 126, 333, 1497
BpuEI CTTGAG 2 cut(s) 730, 786
BsaHI GRCGYC 3 cut(s) 119, 219, 472
BsaJI CCNNGG 4 cut(s) 282, 446, 499, 926
Bsc4I CCNNNNNNNGG 5 cut(s) 452, 711, 1070, 1234, 1528
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 2 cut(s) 1031, 1421
BseBI CCWGG 2 cut(s) 447, 1065
BseDI CCNNGG 4 cut(s) 282, 446, 499, 926
BseGI GGATG 4 cut(s) 86, 158, 495, 1513
BseLI CCNNNNNNNGG 5 cut(s) 452, 711, 1070, 1234, 1528
BseMII CTCAG 1 cut(s) 993
BseNI ACTGG 2 cut(s) 1031, 1421
BseRI GAGGAG 2 cut(s) 1268, 1440
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 5 cut(s) 36, 80, 629, 902, 994
BshNI GGYRCC 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 1486
BslI CCNNNNNNNGG 5 cut(s) 452, 711, 1070, 1234, 1528
BsmAI GTCTC 2 cut(s) 828, 1363
BsmFI GGGAC 1 cut(s) 1486
BsnI GGCC 5 cut(s) 36, 80, 629, 902, 994
BsoBI CYCGRG 1 cut(s) 196
Bsp120I GGGCCC 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 82, 1144
Bsp143I GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
BspACI CCGC 6 cut(s) 213, 225, 510, 848, 1307, 1360
BspANI GGCC 5 cut(s) 36, 80, 629, 902, 994
BspCNI CTCAG 1 cut(s) 994
BspLI GGNNCC 5 cut(s) 79, 80, 220, 645, 924
BspMI ACCTGC 1 cut(s) 1337
BspPI GGATC 5 cut(s) 342, 917, 930, 1017, 1055
BspT107I GGYRCC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 2 cut(s) 1031, 1421
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 4 cut(s) 282, 446, 499, 926
BssMI GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
BssNI GRCGYC 3 cut(s) 119, 219, 472
BssT1I CCWWGG 2 cut(s) 499, 926
Bst2UI CCWGG 2 cut(s) 447, 1065
Bst4CI ACNGT 6 cut(s) 96, 365, 618, 1115, 1477, 1587
Bst6I CTCTTC 3 cut(s) 252, 742, 1063
BstACI GRCGYC 3 cut(s) 119, 219, 472
BstC8I GCNNGC 7 cut(s) 205, 223, 493, 657, 661, 826, 1463
BstDEI CTNAG 3 cut(s) 1002, 1303, 1373
BstF5I GGATG 4 cut(s) 86, 158, 495, 1513
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 3 cut(s) 203, 221, 491
BstKTI GATC 7 cut(s) 337, 754, 817, 925, 1012, 1063, 1125
BstMAI GTCTC 2 cut(s) 828, 1363
BstMBI GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
BstMWI GCNNNNNNNGC 1 cut(s) 721
BstNI CCWGG 2 cut(s) 447, 1065
BstSCI CCNGG 2 cut(s) 445, 1063
BstSFI CTRYAG 2 cut(s) 664, 1586
BstSLI GKGCMC 1 cut(s) 82
BstV1I GCAGC 1 cut(s) 265
BstV2I GAAGAC 4 cut(s) 90, 126, 333, 1497
BstX2I RGATCY 4 cut(s) 334, 751, 922, 1060
BstYI RGATCY 4 cut(s) 334, 751, 922, 1060
BsuRI GGCC 5 cut(s) 36, 80, 629, 902, 994
BtgZI GCGATG 1 cut(s) 1089
BtsCI GGATG 4 cut(s) 86, 158, 495, 1513
BtsI GCAGTG 2 cut(s) 190, 214
BtsIMutI CAGTG 4 cut(s) 190, 214, 614, 1053
BveI ACCTGC 1 cut(s) 1337
Cac8I GCNNGC 7 cut(s) 205, 223, 493, 657, 661, 826, 1463
CaiI CAGNNNCTG 1 cut(s) 1585
CfoI GCGC 3 cut(s) 203, 221, 491
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 5 cut(s) 35, 78, 79, 512, 1079
CseI GACGC 1 cut(s) 713
Csp6I GTAC 3 cut(s) 420, 941, 1255
CviAII CATG 3 cut(s) 1091, 1229, 1574
CviQI GTAC 3 cut(s) 420, 941, 1255
DdeI CTNAG 3 cut(s) 1002, 1303, 1373
DinI GGCGCC 1 cut(s) 220
DpnI GATC 7 cut(s) 336, 753, 816, 924, 1011, 1062, 1124
DpnII GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
DraIII CACNNNGTG 1 cut(s) 1399
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 900
Eam1104I CTCTTC 3 cut(s) 252, 742, 1063
EarI CTCTTC 3 cut(s) 252, 742, 1063
EciI GGCGGA 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 499, 926
Eco24I GRGCYC 2 cut(s) 82, 1144
Eco47I GGWCC 2 cut(s) 512, 1079
Eco57I CTGAAG 2 cut(s) 756, 1077
Eco88I CYCGRG 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 445, 1063
EcoT14I CCWWGG 2 cut(s) 499, 926
EcoT38I GRGCYC 2 cut(s) 82, 1144
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 2 cut(s) 499, 926
FaeI CATG 3 cut(s) 1094, 1232, 1577
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 1 cut(s) 1486
FatI CATG 3 cut(s) 1090, 1228, 1573
FauI CCCGC 1 cut(s) 1300
FbaI TGATCA 1 cut(s) 1122
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 4 cut(s) 73, 145, 482, 1500
FriOI GRGCYC 2 cut(s) 82, 1144
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 1 cut(s) 381
GlaI GCGC 3 cut(s) 202, 220, 490
GluI GCNGC 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 5 cut(s) 36, 80, 629, 902, 994
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 713
HhaI GCGC 3 cut(s) 203, 221, 491
Hin1I GRCGYC 3 cut(s) 119, 219, 472
Hin1II CATG 3 cut(s) 1094, 1232, 1577
Hin6I GCGC 3 cut(s) 201, 219, 489
HinP1I GCGC 3 cut(s) 201, 219, 489
HincII GTYRAC 1 cut(s) 1352
HindII GTYRAC 1 cut(s) 1352
HinfI GANTC 5 cut(s) 176, 442, 1045, 1181, 1291
HpaII CCGG 1 cut(s) 222
HphI GGTGA 4 cut(s) 84, 245, 617, 824
Hpy166II GTNNAC 2 cut(s) 1352, 1509
Hpy188I TCNGA 5 cut(s) 13, 349, 539, 583, 1155
Hpy188III TCNNGA 5 cut(s) 468, 563, 621, 641, 968
Hpy8I GTNNAC 2 cut(s) 1352, 1509
Hpy99I CGWCG 9 cut(s) 56, 112, 121, 124, 127, 130, 133, 148, 462
HpyAV CCTTC 6 cut(s) 123, 161, 671, 755, 1011, 1191
HpyCH4III ACNGT 6 cut(s) 96, 365, 618, 1115, 1477, 1587
HpyCH4IV ACGT 4 cut(s) 119, 472, 571, 1525
HpyCH4V TGCA 5 cut(s) 635, 828, 976, 1346, 1504
HpyF10VI GCNNNNNNNGC 1 cut(s) 721
HpyF3I CTNAG 3 cut(s) 1002, 1303, 1373
HpySE526I ACGT 4 cut(s) 119, 472, 571, 1525
Hsp92I GRCGYC 3 cut(s) 119, 219, 472
Hsp92II CATG 3 cut(s) 1094, 1232, 1577
HspAI GCGC 3 cut(s) 201, 219, 489
KasI GGCGCC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 1122
Kzo9I GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
LmnI GCTCC 2 cut(s) 1281, 1531
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 3 cut(s) 504, 1087, 1333
MaeI CTAG 1 cut(s) 381
MaeII ACGT 4 cut(s) 119, 472, 571, 1525
MaeIII GTNAC 3 cut(s) 311, 868, 1022
MalI GATC 7 cut(s) 336, 753, 816, 924, 1011, 1062, 1124
MboI GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
MflI RGATCY 4 cut(s) 334, 751, 922, 1060
MhlI GDGCHC 2 cut(s) 82, 1144
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 6 cut(s) 532, 584, 741, 1133, 1214, 1283
MluNI TGGCCA 1 cut(s) 902
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 2 cut(s) 185, 1054
MmeI TCCRAC 4 cut(s) 186, 987, 1215, 1546
Mox20I TGGCCA 1 cut(s) 902
MreI CGCCGGCG 1 cut(s) 221
MroNI GCCGGC 1 cut(s) 221
MscI TGGCCA 1 cut(s) 902
MseI TTAA 3 cut(s) 587, 756, 1217
MslI CAYNNNNRTG 1 cut(s) 1095
Msp20I TGGCCA 1 cut(s) 902
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 447, 1065
MvaI CCWGG 2 cut(s) 447, 1065
MwoI GCNNNNNNNGC 1 cut(s) 721
NaeI GCCGGC 1 cut(s) 223
NarI GGCGCC 1 cut(s) 219
NdeII GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
NgoMIV GCCGGC 1 cut(s) 221
NlaIII CATG 3 cut(s) 1094, 1232, 1577
NlaIV GGNNCC 5 cut(s) 79, 80, 220, 645, 924
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 868
PcsI WCGNNNNNNNCGW 8 cut(s) 51, 113, 116, 122, 125, 128, 134, 568
PdiI GCCGGC 1 cut(s) 223
PfeI GAWTC 3 cut(s) 442, 1181, 1291
PflFI GACNNNGTC 1 cut(s) 121
PflMI CCANNNNNTGG 3 cut(s) 452, 1234, 1528
PfoI TCCNGGA 1 cut(s) 1063
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 2 cut(s) 184, 1053
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 2 cut(s) 184, 1053
PsiI TTATAA 1 cut(s) 912
Psp1406I AACGTT 2 cut(s) 571, 1525
Psp6I CCWGG 2 cut(s) 445, 1063
PspGI CCWGG 2 cut(s) 445, 1063
PspN4I GGNNCC 5 cut(s) 79, 80, 220, 645, 924
PspOMI GGGCCC 1 cut(s) 78
PspPI GGNCC 5 cut(s) 35, 78, 79, 512, 1079
PstNI CAGNNNCTG 1 cut(s) 1585
PsuI RGATCY 4 cut(s) 334, 751, 922, 1060
PsyI GACNNNGTC 1 cut(s) 121
RsaI GTAC 3 cut(s) 421, 942, 1256
RsaNI GTAC 3 cut(s) 420, 941, 1255
RseI CAYNNNNRTG 1 cut(s) 1095
SaqAI TTAA 3 cut(s) 587, 756, 1217
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 7 cut(s) 334, 751, 814, 922, 1009, 1060, 1122
Sau96I GGNCC 5 cut(s) 35, 78, 79, 512, 1079
SchI GAGTC 2 cut(s) 185, 1054
ScrFI CCNGG 2 cut(s) 447, 1065
SduI GDGCHC 2 cut(s) 82, 1144
SfaNI GCATC 3 cut(s) 504, 1087, 1333
SfcI CTRYAG 2 cut(s) 664, 1586
SfoI GGCGCC 1 cut(s) 220
SgrAI CRCCGGYG 1 cut(s) 221
SinI GGWCC 2 cut(s) 512, 1079
SmiMI CAYNNNNRTG 1 cut(s) 1095
SmlI CTYRAG 2 cut(s) 709, 801
SmoI CTYRAG 2 cut(s) 709, 801
SpeI ACTAGT 1 cut(s) 380
Sse9I AATT 6 cut(s) 532, 584, 741, 1133, 1214, 1283
SsiI CCGC 6 cut(s) 213, 225, 510, 848, 1307, 1360
SspDI GGCGCC 1 cut(s) 218
SspI AATATT 1 cut(s) 844
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 2 cut(s) 445, 1063
StyI CCWWGG 2 cut(s) 499, 926
TaaI ACNGT 6 cut(s) 96, 365, 618, 1115, 1477, 1587
TaiI ACGT 4 cut(s) 122, 475, 574, 1528
TaqI TCGA 3 cut(s) 116, 505, 1012
TaqII GACCGA 1 cut(s) 1096
TasI AATT 6 cut(s) 532, 584, 741, 1133, 1214, 1283
TatI WGTACW 1 cut(s) 940
TfiI GAWTC 3 cut(s) 442, 1181, 1291
Tru1I TTAA 3 cut(s) 587, 756, 1217
Tru9I TTAA 3 cut(s) 587, 756, 1217
TscAI CASTG 4 cut(s) 190, 214, 621, 1060
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 7 cut(s) 147, 369, 582, 774, 851, 1421, 1503
TspRI CASTG 4 cut(s) 190, 214, 621, 1060
Tth111I GACNNNGTC 1 cut(s) 121
Van91I CCANNNNNTGG 3 cut(s) 452, 1234, 1528
VpaK11BI GGWCC 2 cut(s) 512, 1079
XapI RAATTY 2 cut(s) 532, 1283
XspI CTAG 1 cut(s) 381
ZraI GACGTC 2 cut(s) 120, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.