FvH4_5g20680

RAI1 like PD-(D/E)XK nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008690
Physical Location & Seq
Reverse (-)
12277860 .. 12283203
5344 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20680.t1

Sequence Viewer

Length: 1572 bp
ATGGATTTCGCAAAGCAGCACGCAGATATCTTCGGCGAGGACGATGACGATGAACCCACCAACCAAGACGGAGGGACCCATTCCTCCTCCCCCTCCTCCTCCTCATCCTCCTCCTCTTCCTCGTCTTCTTCATCCTCCGCCGCTTCCTCTTCCGGCAGCGAGAAAGCCCAGTCCAGCAGCGGCAATAGCCGAAGCGGGGCCACCACCGGCGGCTGTGGCGCCGCTGAGGATGAGGAGGAGCACGGCGAAGAGGCCACCACGAACAATGAGTATCACGATAGGGCCGATTTTGGGGACGATGATAAGGATCTGTTTGGTTCTGATAATGAAGAATACACTAGAACCCCTGCTACTAGCCCTTTCCTTATTCCTGTGTTGCCTGTGATACGGAATAATAATCATAATCAGGGAAGAGGGGGTTTTGGGCGTGGTGGTCGATGGCAACCCGGGCATCAGAATGACAGAGGAGCTGGCATTCTTCCCCGACCTGGACCTTATCCGCAGAGGGGGCATAATTATGGCTATGGGAAGTTCTCGAATGGTCGTCATGATGAACGTTTCGTTTCTGAGCTGAGACTGTCAAAGAGTGAAGAAACGCTGTCGAGAAAGGTTATTCAGATTCAGGAACCATGTGAACTTGCTTGCTATAGTCGGGTAGAAGGGGGAGATGTCATCTTTGATGAGAGCAGCTTGAGGCTTTTCAAGCGGCTTATTACTGAAGATATTGGAGCCGATCTTAATCAAGGCTTTGATACTTTCACAGCAAAGAATGATTTGGGCTCTCAAGGTTTTGGTGATCTTCTTGCCTGCATCAGAGACAAGAATATTCCACTTCAAAATATTCATTTTGTGACTTATCGCAACAACCTTAATAAGATATTAGCCACTGCTTATATTCGGAATGAGCCTTGGGAAATGGGAGTGCATAAAAGGAATGGGGTTGTCTATCTTGATGTACATAAACTTCCAGAAAGGCCACAAAATGAAATGGATCGTCGAAGGTGTTACTGGGGATACTGTTTTGAGAGTCTTGCCACTGAAGATCCAGGAAGAGGTGATGGAGAGTTGATACATCACGTAGATGCCAATATTGAATACTGTTCAGTAATTAAAACCAAATTGGGGGCTCATCGTATTCTGATGGGCGCTGAAATGGATTGCTGTGATTCAACTGATGATGGAAGGAGATTTTACGTGGAGTTGAAGACAAGTCGTGAGTTGGAGTATCATACCGAGGAAAGATTCGAGAGAGAGAAGCTCCTCAAGTTTTGGATTCAATCATTCTTAGCAGGCGTGCCCTATATTGTAATTGGATTTAGGGATGATTCAGGTAGACTTGTCCGCACAGAGAGACTAAGAACCAAAGATATAACACAGAGAGTGAAAACGAAGAACTACTGGCAGGGAGGAGTTTGCTTGGCATTTGCTGATGAGGTATTATGCTGGCTGTATGGAACAGTTAAAGAGAATGAAGATTACATATTGCAGTTTGCTCCTCCTTTCCAGCGTTTGGAGCTTCTACAAGCTCAATCTTGCCCAGAGGCAATCACTAACCATGTAGAGCAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAI1

Protein Analysis

524

Amino Acids

58.78

Weight (kDa)

5.2

Isoelectric Point (pI)

55.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAI1 PF08652 208 - 510 4.3e-85 RAI1 like PD-(D/E)XK nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1570
AccB1I GGYRCC 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 1510
AccI GTMKAC 1 cut(s) 1333
AciI CCGC 9 cut(s) 138, 141, 180, 195, 210, 222, 500, 706, 1342
AclI AACGTT 1 cut(s) 556
AclWI GGATC 3 cut(s) 315, 999, 1037
AcuI CTGAAG 2 cut(s) 738, 1059
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 1 cut(s) 957
AfiI CCNNNNNNNGG 7 cut(s) 196, 291, 488, 506, 1052, 1122, 1510
AgsI TTSAA 6 cut(s) 703, 836, 1094, 1170, 1204, 1277
AjnI CCWGG 2 cut(s) 487, 1045
AluBI AGCT 6 cut(s) 470, 571, 690, 1258, 1516, 1526
AluI AGCT 6 cut(s) 470, 571, 690, 1258, 1516, 1526
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 3 cut(s) 568, 810, 1345
AlwI GGATC 3 cut(s) 315, 999, 1037
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 4 cut(s) 198, 252, 282, 974
ApeKI GCWGC 4 cut(s) 16, 156, 177, 687
AspLEI GCGC 2 cut(s) 221, 1148
AspS9I GGNCC 4 cut(s) 75, 198, 282, 491
AsuC2I CCSGG 2 cut(s) 447, 448
AsuHPI GGTGA 2 cut(s) 806, 1067
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 2 cut(s) 75, 491
BaeGI GKGCMC 1 cut(s) 1299
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 2 cut(s) 782, 1129
BarI GAAGNNNNNNTAC 2 cut(s) 948, 980
BbsI GAAGAC 2 cut(s) 117, 1211
Bbv12I GWGCWC 1 cut(s) 243
BbvCI CCTCAGC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 28, 168, 189, 699
BccI CCATC 4 cut(s) 432, 1052, 1135, 1172
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 2 cut(s) 489, 1047
BciVI GTATCC 1 cut(s) 1007
BcnI CCSGG 2 cut(s) 447, 448
BcoDI GTCTC 3 cut(s) 568, 810, 1345
BfaI CTAG 2 cut(s) 339, 354
BfmI CTRYAG 1 cut(s) 646
BfoI RGCGCY 2 cut(s) 222, 1149
BfuI GTATCC 1 cut(s) 1007
BisI GCNGC 9 cut(s) 17, 141, 157, 178, 181, 211, 222, 688, 707
BlsI GCNGC 9 cut(s) 18, 142, 158, 179, 182, 212, 223, 689, 708
Bme1390I CCNGG 4 cut(s) 447, 448, 489, 1047
Bme18I GGWCC 2 cut(s) 75, 491
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 4 cut(s) 75, 198, 282, 491
BmiI GGNNCC 6 cut(s) 76, 77, 199, 220, 627, 730
BmrFI CCNGG 4 cut(s) 447, 448, 489, 1047
BmrI ACTGGG 2 cut(s) 163, 1018
BmsI GCATC 3 cut(s) 460, 819, 1072
BmuI ACTGGG 2 cut(s) 163, 1018
BpiI GAAGAC 2 cut(s) 117, 1211
BplI GAGNNNNNCTC 2 cut(s) 1242, 1274
Bpu10I CCTNAGC 1 cut(s) 225
BpuEI CTTGAG 3 cut(s) 712, 768, 1247
BpuMI CCSGG 2 cut(s) 447, 448
BsaAI YACGTR 2 cut(s) 1078, 1195
BsaBI GATNNNNATC 2 cut(s) 306, 738
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 446, 908, 1233
Bsc4I CCNNNNNNNGG 7 cut(s) 196, 291, 488, 506, 1052, 1122, 1510
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 169, 1013, 1403
Bse8I GATNNNNATC 2 cut(s) 306, 738
BseBI CCWGG 2 cut(s) 489, 1047
BseDI CCNNGG 3 cut(s) 446, 908, 1233
BseGI GGATG 4 cut(s) 104, 131, 235, 1327
BseJI GATNNNNATC 2 cut(s) 306, 738
BseLI CCNNNNNNNGG 7 cut(s) 196, 291, 488, 506, 1052, 1122, 1510
BseMII CTCAG 3 cut(s) 216, 558, 563
BseNI ACTGG 3 cut(s) 169, 1013, 1403
BseSI GKGCMC 1 cut(s) 1299
BseXI GCAGC 4 cut(s) 28, 168, 189, 699
BshFI GGCC 4 cut(s) 200, 254, 284, 976
BshNI GGYRCC 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 3 cut(s) 153, 207, 447
BslFI GGGAC 2 cut(s) 88, 308
BslI CCNNNNNNNGG 7 cut(s) 196, 291, 488, 506, 1052, 1122, 1510
BsmAI GTCTC 3 cut(s) 568, 810, 1345
BsmFI GGGAC 2 cut(s) 88, 308
BsmI GAATGC 1 cut(s) 474
BsnI GGCC 4 cut(s) 200, 254, 284, 976
BsoBI CYCGRG 1 cut(s) 446
Bsp1286I GDGCHC 4 cut(s) 243, 782, 1129, 1299
Bsp1407I TGTACA 1 cut(s) 955
Bsp143I GATC 5 cut(s) 307, 733, 796, 991, 1042
BspACI CCGC 9 cut(s) 138, 141, 180, 195, 210, 222, 500, 706, 1342
BspANI GGCC 4 cut(s) 200, 254, 284, 976
BspCNI CTCAG 3 cut(s) 217, 559, 564
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 6 cut(s) 76, 77, 199, 220, 627, 730
BspPI GGATC 3 cut(s) 315, 999, 1037
BspT107I GGYRCC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 206
BsrGI TGTACA 1 cut(s) 955
BsrI ACTGG 3 cut(s) 169, 1013, 1403
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 3 cut(s) 446, 908, 1233
BssMI GATC 5 cut(s) 307, 733, 796, 991, 1042
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 908
Bst2UI CCWGG 2 cut(s) 489, 1047
Bst4CI ACNGT 4 cut(s) 579, 1019, 1100, 1459
Bst6I CTCTTC 5 cut(s) 121, 154, 243, 406, 1045
BstACI GRCGYC 1 cut(s) 219
BstAUI TGTACA 1 cut(s) 955
BstBAI YACGTR 2 cut(s) 1078, 1195
BstC8I GCNNGC 7 cut(s) 21, 472, 643, 808, 1291, 1295, 1445
BstDEI CTNAG 5 cut(s) 225, 567, 572, 1285, 1355
BstF5I GGATG 4 cut(s) 104, 131, 235, 1327
BstH2I RGCGCY 2 cut(s) 222, 1149
BstHHI GCGC 2 cut(s) 221, 1148
BstKTI GATC 5 cut(s) 310, 736, 799, 994, 1045
BstMAI GTCTC 3 cut(s) 568, 810, 1345
BstMBI GATC 5 cut(s) 307, 733, 796, 991, 1042
BstMWI GCNNNNNNNGC 6 cut(s) 186, 216, 448, 508, 703, 1513
BstNI CCWGG 2 cut(s) 489, 1047
BstSCI CCNGG 4 cut(s) 445, 446, 487, 1045
BstSFI CTRYAG 1 cut(s) 646
BstSLI GKGCMC 1 cut(s) 1299
BstV1I GCAGC 4 cut(s) 28, 168, 189, 699
BstV2I GAAGAC 2 cut(s) 117, 1211
BstX2I RGATCY 2 cut(s) 307, 1042
BstYI RGATCY 2 cut(s) 307, 1042
BsuI GTATCC 1 cut(s) 1007
BsuRI GGCC 4 cut(s) 200, 254, 284, 976
BtsCI GGATG 4 cut(s) 104, 131, 235, 1327
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 2 cut(s) 885, 1035
Cac8I GCNNGC 7 cut(s) 21, 472, 643, 808, 1291, 1295, 1445
CciI TCATGA 1 cut(s) 547
CfoI GCGC 2 cut(s) 221, 1148
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 4 cut(s) 75, 198, 282, 491
Cfr9I CCCGGG 1 cut(s) 446
Csp6I GTAC 1 cut(s) 956
CviAII CATG 3 cut(s) 548, 630, 1556
CviQI GTAC 1 cut(s) 956
DdeI CTNAG 5 cut(s) 225, 567, 572, 1285, 1355
DinI GGCGCC 1 cut(s) 220
DpnI GATC 5 cut(s) 309, 735, 798, 993, 1044
DpnII GATC 5 cut(s) 307, 733, 796, 991, 1042
Eam1104I CTCTTC 5 cut(s) 121, 154, 243, 406, 1045
EarI CTCTTC 5 cut(s) 121, 154, 243, 406, 1045
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 908
Eco24I GRGCYC 2 cut(s) 782, 1129
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 2 cut(s) 75, 491
Eco57I CTGAAG 2 cut(s) 738, 1059
Eco88I CYCGRG 1 cut(s) 446
EcoO109I RGGNCCY 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 487, 1045
EcoRV GATATC 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 908
EcoT38I GRGCYC 2 cut(s) 782, 1129
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 908
FaeI CATG 3 cut(s) 551, 633, 1559
FaqI GGGAC 2 cut(s) 88, 308
FatI CATG 3 cut(s) 547, 629, 1555
FauI CCCGC 1 cut(s) 188
FblI GTMKAC 1 cut(s) 1333
Fnu4HI GCNGC 9 cut(s) 17, 141, 157, 178, 181, 211, 222, 688, 707
FokI GGATG 4 cut(s) 91, 118, 242, 1334
FriOI GRGCYC 2 cut(s) 782, 1129
Fsp4HI GCNGC 9 cut(s) 17, 141, 157, 178, 181, 211, 222, 688, 707
FspBI CTAG 2 cut(s) 339, 354
GlaI GCGC 2 cut(s) 220, 1147
GluI GCNGC 9 cut(s) 17, 141, 157, 178, 181, 211, 222, 688, 707
HaeII RGCGCY 2 cut(s) 222, 1149
HaeIII GGCC 4 cut(s) 200, 254, 284, 976
HapII CCGG 3 cut(s) 153, 207, 447
HhaI GCGC 2 cut(s) 221, 1148
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 3 cut(s) 551, 633, 1559
Hin6I GCGC 2 cut(s) 219, 1146
HinP1I GCGC 2 cut(s) 219, 1146
HinfI GANTC 6 cut(s) 619, 1027, 1166, 1242, 1273, 1325
HpaII CCGG 3 cut(s) 153, 207, 447
HphI GGTGA 2 cut(s) 806, 1067
Hpy166II GTNNAC 2 cut(s) 635, 1334
Hpy188I TCNGA 7 cut(s) 322, 456, 568, 618, 815, 900, 1140
Hpy188III TCNNGA 9 cut(s) 275, 535, 548, 603, 623, 950, 968, 1214, 1246
Hpy8I GTNNAC 2 cut(s) 635, 1334
Hpy99I CGWCG 1 cut(s) 999
HpyAV CCTTC 3 cut(s) 653, 993, 1176
HpyCH4III ACNGT 4 cut(s) 579, 1019, 1100, 1459
HpyCH4IV ACGT 3 cut(s) 556, 1077, 1194
HpyCH4V TGCA 3 cut(s) 810, 925, 1486
HpyF10VI GCNNNNNNNGC 6 cut(s) 186, 216, 448, 508, 703, 1513
HpyF3I CTNAG 5 cut(s) 225, 567, 572, 1285, 1355
HpySE526I ACGT 3 cut(s) 556, 1077, 1194
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 3 cut(s) 551, 633, 1559
HspAI GCGC 2 cut(s) 219, 1146
KasI GGCGCC 1 cut(s) 218
KflI GGGWCCC 1 cut(s) 75
Kzo9I GATC 5 cut(s) 307, 733, 796, 991, 1042
LmnI GCTCC 6 cut(s) 238, 467, 728, 1263, 1498, 1513
Lsp1109I GCAGC 4 cut(s) 28, 168, 189, 699
LweI GCATC 3 cut(s) 460, 819, 1072
MaeI CTAG 2 cut(s) 339, 354
MaeII ACGT 3 cut(s) 556, 1077, 1194
MaeIII GTNAC 2 cut(s) 850, 1004
MalI GATC 5 cut(s) 309, 735, 798, 993, 1044
MboI GATC 5 cut(s) 307, 733, 796, 991, 1042
MflI RGATCY 2 cut(s) 307, 1042
MhlI GDGCHC 4 cut(s) 243, 782, 1129, 1299
MluCI AATT 5 cut(s) 514, 1107, 1118, 1308, 1565
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 1 cut(s) 1036
MmeI TCCRAC 1 cut(s) 1200
MseI TTAA 4 cut(s) 738, 870, 1110, 1461
MslI CAYNNNNRTG 3 cut(s) 456, 516, 1080
MspA1I CMGCKG 2 cut(s) 180, 224
MspI CCGG 3 cut(s) 153, 207, 447
MspR9I CCNGG 4 cut(s) 447, 448, 489, 1047
Mva1269I GAATGC 1 cut(s) 474
MvaI CCWGG 2 cut(s) 489, 1047
MwoI GCNNNNNNNGC 6 cut(s) 186, 216, 448, 508, 703, 1513
NarI GGCGCC 1 cut(s) 219
NciI CCSGG 2 cut(s) 447, 448
NdeII GATC 5 cut(s) 307, 733, 796, 991, 1042
NlaIII CATG 3 cut(s) 551, 633, 1559
NlaIV GGNNCC 6 cut(s) 76, 77, 199, 220, 627, 730
NmuCI GTSAC 1 cut(s) 850
PagI TCATGA 1 cut(s) 547
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 282
PctI GAATGC 1 cut(s) 474
PfeI GAWTC 5 cut(s) 619, 1166, 1242, 1273, 1325
PflMI CCANNNNNTGG 1 cut(s) 1510
PfoI TCCNGGA 1 cut(s) 1045
PkrI GCNGC 9 cut(s) 18, 142, 158, 179, 182, 212, 223, 689, 708
PleI GAGTC 1 cut(s) 1035
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 1035
Ppu21I YACGTR 2 cut(s) 1078, 1195
PpuMI RGGWCCY 1 cut(s) 75
PsiI TTATAA 1 cut(s) 1570
Psp1406I AACGTT 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 487, 1045
PspGI CCWGG 2 cut(s) 487, 1045
PspN4I GGNNCC 6 cut(s) 76, 77, 199, 220, 627, 730
PspPI GGNCC 4 cut(s) 75, 198, 282, 491
PspPPI RGGWCCY 1 cut(s) 75
PsrI GAACNNNNNNTAC 2 cut(s) 334, 366
PsuI RGATCY 2 cut(s) 307, 1042
RsaI GTAC 1 cut(s) 957
RsaNI GTAC 1 cut(s) 956
RseI CAYNNNNRTG 3 cut(s) 456, 516, 1080
SaqAI TTAA 4 cut(s) 738, 870, 1110, 1461
SatI GCNGC 9 cut(s) 17, 141, 157, 178, 181, 211, 222, 688, 707
Sau3AI GATC 5 cut(s) 307, 733, 796, 991, 1042
Sau96I GGNCC 4 cut(s) 75, 198, 282, 491
SchI GAGTC 1 cut(s) 1036
ScrFI CCNGG 4 cut(s) 447, 448, 489, 1047
SduI GDGCHC 4 cut(s) 243, 782, 1129, 1299
SfaNI GCATC 3 cut(s) 460, 819, 1072
SfcI CTRYAG 1 cut(s) 646
SfoI GGCGCC 1 cut(s) 220
SgrAI CRCCGGYG 1 cut(s) 206
SinI GGWCC 2 cut(s) 75, 491
SmaI CCCGGG 1 cut(s) 448
SmiMI CAYNNNNRTG 3 cut(s) 456, 516, 1080
SmlI CTYRAG 3 cut(s) 691, 783, 1262
SmoI CTYRAG 3 cut(s) 691, 783, 1262
Sse9I AATT 5 cut(s) 514, 1107, 1118, 1308, 1565
SsiI CCGC 9 cut(s) 138, 141, 180, 195, 210, 222, 500, 706, 1342
SspDI GGCGCC 1 cut(s) 218
SspI AATATT 3 cut(s) 826, 841, 1090
SspMI CTAG 2 cut(s) 339, 354
StyD4I CCNGG 4 cut(s) 445, 446, 487, 1045
StyI CCWWGG 1 cut(s) 908
TaaI ACNGT 4 cut(s) 579, 1019, 1100, 1459
TaiI ACGT 3 cut(s) 559, 1080, 1197
TaqI TCGA 5 cut(s) 436, 536, 602, 997, 1245
TasI AATT 5 cut(s) 514, 1107, 1118, 1308, 1565
TatI WGTACW 1 cut(s) 955
TauI GCSGC 5 cut(s) 143, 183, 213, 224, 709
TfiI GAWTC 5 cut(s) 619, 1166, 1242, 1273, 1325
Tru1I TTAA 4 cut(s) 738, 870, 1110, 1461
Tru9I TTAA 4 cut(s) 738, 870, 1110, 1461
TscAI CASTG 2 cut(s) 892, 1042
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 4 cut(s) 16, 156, 177, 687
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 7 cut(s) 66, 120, 342, 567, 833, 999, 1485
TspGWI ACGGA 2 cut(s) 84, 403
TspMI CCCGGG 1 cut(s) 446
TspRI CASTG 2 cut(s) 892, 1042
Van91I CCANNNNNTGG 1 cut(s) 1510
VpaK11BI GGWCC 2 cut(s) 75, 491
XmaI CCCGGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 1333
XspI CTAG 2 cut(s) 339, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.