AT4G18060

Variant SH3 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010268
Physical Location & Seq
Reverse (-)
10026650 .. 10030033
3384 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18060.1

Sequence Viewer

Length: 1056 bp
ATGGATGCGTTTAGAAGACAAGCGAGTAAGCTTAGAGACCAAGTCGCAAAGCAGCAACTGGCTGTGATAAAGCAATTCAGTGGGACTGGTTATGAAAGCTCAGATGTAATGGTGATTGATGAGCTTGAAATGCAAAGGCATCATCAACTCGATAAACTTTACCGCTCTACTCGTTCTGCAAAGGAGTTCCAGAGAGATATTGTTAAAGCAGCTGAGGCATTTACCACGATCGGGCTCAGACACATTGAAGCAGGAACCAAGTTGTCTGAAGACTGTTGTAGGTATGGGAACGAAAATAGTCAGAACATTGATGAGAATATACTGGCCAAGGCTGCAGCTATATACGGTGATGCACGGAAACATGTAGACAAAGAACAAGAAGACTTCAACAAGCTTTTGGCTTCTCAGGTTTTGGATCCTCTAAGAGCTATGGTTGCCGGTTCTCCTCTTGAAGATGCTCGTCATCTTGCTCAACGTTACAGCCGCATGAGACAAGAAGCAGAGACACATGCAACGGAAGTCTCTAGACGGCAAGCACGTGTAAGAGAAGCTCCGATTCCAGAAAACGTAGCAAAGCTTCAACTAGCGGAAGCAAAAATGCAAGAGCTAAAAGCAAATATGGCAGTCCTTGGTAAAGAAGCAACTGCTGCATTAGCCGCTGTTGAATCACAGCAACATAGACTCACTTTCCAGAGGCTTGTGGCTATGGTTGAAGGGGAGAAGAATTATCACTTAAGAATAGCAGCCATCCTCAGTGACATTGAAGCCGAGATGGTAACCGAGAAACAACACAAAGAATCTGCTCCTCCTGCGATTCCAACAGAGAACGGCTCAGAGAAAACGTCATACTTTCTTGCTGAAGTGATTCATCCATTTTCCGCTGCTTCAGAGAAAGAGTTAGACTTGGACAAAGGAGATTACATCGTTGTCCGAAAGGTGAGCCAAACCGGTTGGGCTGAAGGAGAATGCAAAGGCAAAGCTGGTTGGTTTCCTATGGCTTACATTGAGAAGCGACAGAGACTTCCCACTACCAACTTTGCTGCTGAAGTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SH3_1 SH3_9

Protein Analysis

351

Amino Acids

39.53

Weight (kDa)

6.4

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_9 PF14604 288 - 336 2.6e-09 Variant SH3 domain
SH3_1 PF00018 288 - 331 9.4e-09 SH3 domain
SH3_2 PF07653 294 - 337 3.7e-07 Variant SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18060
fragaria_vesca FvH4_3g06560 FvH4_3g06560 FvH4_3g06560 FvH4_3g33220
malus_domestica MD05G1292300.v1.1 MD10G1268100.v1.1
prunus_persica Prupe.4G073100_v2.0.a1
pyrus_communis pycom05g26860 pycom10g22440
rosa_chinensis RchiOBHm_Chr5g0012601
rosa_laevigata RLG00000031928
rosa_multiflora Rmu_sc0003577.1_g000008
rosa_roxburghii Rroxscaffold_1G00064070
rosa_rugosa Rorug05G0002800 Rorug05G0002900
rosa_samantha Rh5AG096700 Rh5BG093000 Rh5CG105800 Rh5DG092400
rosa_wichuraiana Rw5G008470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 165
AccI GTMKAC 1 cut(s) 366
AciI CCGC 5 cut(s) 163, 484, 587, 657, 879
AclI AACGTT 1 cut(s) 475
AclWI GGATC 2 cut(s) 410, 423
AcoI YGGCCR 1 cut(s) 324
AcuI CTGAAG 4 cut(s) 288, 870, 879, 978
AcvI CACGTG 1 cut(s) 539
AfiI CCNNNNNNNGG 1 cut(s) 231
AflII CTTAAG 1 cut(s) 733
AflIII ACRYGT 2 cut(s) 361, 538
AgeI ACCGGT 1 cut(s) 947
AgsI TTSAA 8 cut(s) 128, 248, 388, 452, 581, 665, 713, 764
Alw26I GTCTC 5 cut(s) 30, 484, 497, 526, 1012
AlwI GGATC 2 cut(s) 410, 423
AlwNI CAGNNNCTG 1 cut(s) 58
AoxI GGCC 1 cut(s) 324
ApeKI GCWGC 8 cut(s) 52, 209, 332, 335, 647, 743, 881, 1040
AsiGI ACCGGT 1 cut(s) 947
Asp700I GAANNNNTTC 1 cut(s) 864
AsuHPI GGTGA 3 cut(s) 124, 359, 949
BalI TGGCCA 1 cut(s) 326
BamHI GGATCC 1 cut(s) 415
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 561, 593
BbrPI CACGTG 1 cut(s) 539
BbsI GAAGAC 3 cut(s) 22, 276, 387
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 8 cut(s) 64, 221, 319, 347, 634, 755, 868, 1027
BccI CCATC 2 cut(s) 755, 766
BceAI ACGGC 2 cut(s) 545, 844
BcoDI GTCTC 5 cut(s) 30, 484, 497, 526, 1012
BfaI CTAG 2 cut(s) 525, 584
BfmI CTRYAG 1 cut(s) 333
BfrI CTTAAG 1 cut(s) 733
BmiI GGNNCC 2 cut(s) 256, 417
BmsI GCATC 3 cut(s) 148, 340, 445
BpiI GAAGAC 3 cut(s) 22, 276, 387
BplI GAGNNNNNCTC 2 cut(s) 815, 847
Bpu10I CCTNAGC 1 cut(s) 213
BsaAI YACGTR 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 327, 628
BsaWI WCCGGW 1 cut(s) 947
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse118I RCCGGY 2 cut(s) 437, 947
Bse1I ACTGG 3 cut(s) 63, 91, 327
BseDI CCNNGG 2 cut(s) 327, 628
BseGI GGATG 3 cut(s) 10, 747, 868
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMII CTCAG 6 cut(s) 114, 204, 250, 419, 766, 846
BseNI ACTGG 3 cut(s) 63, 91, 327
BseRI GAGGAG 2 cut(s) 435, 795
BseXI GCAGC 8 cut(s) 64, 221, 319, 347, 634, 755, 868, 1027
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 1 cut(s) 326
BshTI ACCGGT 1 cut(s) 947
BsiEI CGRYCG 1 cut(s) 231
BsiSI CCGG 2 cut(s) 438, 948
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 5 cut(s) 30, 484, 497, 526, 1012
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 971
BsnI GGCC 1 cut(s) 326
Bso31I GGTCTC 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 228, 415
BspACI CCGC 5 cut(s) 163, 484, 587, 657, 879
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 6 cut(s) 113, 205, 249, 418, 765, 845
BspLI GGNNCC 2 cut(s) 256, 417
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 2 cut(s) 410, 423
BspTI CTTAAG 1 cut(s) 733
BspTNI GGTCTC 1 cut(s) 30
BsrBI CCGCTC 1 cut(s) 165
BsrFI RCCGGY 2 cut(s) 437, 947
BsrI ACTGG 3 cut(s) 63, 91, 327
BssAI RCCGGY 2 cut(s) 437, 947
BssECI CCNNGG 2 cut(s) 327, 628
BssMI GATC 2 cut(s) 228, 415
BssT1I CCWWGG 2 cut(s) 327, 628
Bst4CI ACNGT 2 cut(s) 275, 347
BstAFI CTTAAG 1 cut(s) 733
BstAPI GCANNNNNTGC 1 cut(s) 647
BstBAI YACGTR 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 534
BstDEI CTNAG 8 cut(s) 32, 100, 213, 236, 405, 422, 752, 832
BstEII GGTNACC 1 cut(s) 775
BstF5I GGATG 3 cut(s) 10, 747, 868
BstKTI GATC 2 cut(s) 231, 418
BstMAI GTCTC 5 cut(s) 30, 484, 497, 526, 1012
BstMBI GATC 2 cut(s) 228, 415
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 9 cut(s) 130, 215, 332, 434, 620, 647, 653, 656, 809
BstNSI RCATGY 2 cut(s) 365, 512
BstPI GGTNACC 1 cut(s) 775
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 8 cut(s) 64, 221, 319, 347, 634, 755, 868, 1027
BstV2I GAAGAC 3 cut(s) 22, 276, 387
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 1 cut(s) 326
BtsCI GGATG 3 cut(s) 10, 747, 868
BtsIMutI CAGTG 2 cut(s) 85, 760
Cac8I GCNNGC 1 cut(s) 534
CaiI CAGNNNCTG 1 cut(s) 58
Cfr10I RCCGGY 2 cut(s) 437, 947
CspAI ACCGGT 1 cut(s) 947
CviAII CATG 3 cut(s) 362, 487, 509
DdeI CTNAG 8 cut(s) 32, 100, 213, 236, 405, 422, 752, 832
DpnI GATC 2 cut(s) 230, 417
DpnII GATC 2 cut(s) 228, 415
EaeI YGGCCR 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 327, 628
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 1 cut(s) 30
Eco57I CTGAAG 4 cut(s) 288, 870, 879, 978
Eco72I CACGTG 1 cut(s) 539
Eco91I GGTNACC 1 cut(s) 775
EcoO65I GGTNACC 1 cut(s) 775
EcoT14I CCWWGG 2 cut(s) 327, 628
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 2 cut(s) 327, 628
FaeI CATG 3 cut(s) 365, 490, 512
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 361, 486, 508
FblI GTMKAC 1 cut(s) 366
FokI GGATG 3 cut(s) 17, 734, 855
FriOI GRGCYC 1 cut(s) 237
FspBI CTAG 2 cut(s) 525, 584
HaeIII GGCC 1 cut(s) 326
HapII CCGG 2 cut(s) 438, 948
Hin1II CATG 3 cut(s) 365, 490, 512
HindIII AAGCTT 3 cut(s) 29, 392, 575
HinfI GANTC 6 cut(s) 556, 665, 681, 797, 814, 865
HpaII CCGG 2 cut(s) 438, 948
HphI GGTGA 3 cut(s) 124, 359, 949
Hpy166II GTNNAC 2 cut(s) 367, 1051
Hpy188I TCNGA 8 cut(s) 103, 239, 268, 303, 555, 835, 889, 932
Hpy188III TCNNGA 5 cut(s) 190, 449, 525, 560, 691
Hpy8I GTNNAC 2 cut(s) 367, 1051
HpyAV CCTTC 2 cut(s) 707, 953
HpyCH4III ACNGT 2 cut(s) 275, 347
HpyCH4IV ACGT 4 cut(s) 475, 538, 567, 842
HpyCH4V TGCA 8 cut(s) 133, 179, 335, 353, 512, 601, 650, 969
HpyF10VI GCNNNNNNNGC 9 cut(s) 130, 215, 332, 434, 620, 647, 653, 656, 809
HpyF3I CTNAG 8 cut(s) 32, 100, 213, 236, 405, 422, 752, 832
HpySE526I ACGT 4 cut(s) 475, 538, 567, 842
Hsp92II CATG 3 cut(s) 365, 490, 512
Kzo9I GATC 2 cut(s) 228, 415
LmnI GCTCC 2 cut(s) 556, 808
Lsp1109I GCAGC 8 cut(s) 64, 221, 319, 347, 634, 755, 868, 1027
LweI GCATC 3 cut(s) 148, 340, 445
MaeI CTAG 2 cut(s) 525, 584
MaeII ACGT 4 cut(s) 475, 538, 567, 842
MaeIII GTNAC 3 cut(s) 476, 755, 775
MalI GATC 2 cut(s) 230, 417
MbiI CCGCTC 1 cut(s) 165
MboI GATC 2 cut(s) 228, 415
MboII GAAGA 5 cut(s) 27, 281, 392, 464, 733
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 237
MlsI TGGCCA 1 cut(s) 326
MluCI AATT 2 cut(s) 74, 724
MluNI TGGCCA 1 cut(s) 326
MlyI GAGTC 1 cut(s) 675
MmeI TCCRAC 1 cut(s) 842
MnlI CCTC 6 cut(s) 208, 429, 456, 687, 761, 816
Mox20I TGGCCA 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 864
MscI TGGCCA 1 cut(s) 326
MseI TTAA 2 cut(s) 204, 734
Msp20I TGGCCA 1 cut(s) 326
MspA1I CMGCKG 3 cut(s) 212, 659, 881
MspCI CTTAAG 1 cut(s) 733
MspI CCGG 2 cut(s) 438, 948
Mva1269I GAATGC 1 cut(s) 971
MwoI GCNNNNNNNGC 9 cut(s) 130, 215, 332, 434, 620, 647, 653, 656, 809
NdeII GATC 2 cut(s) 228, 415
NlaIII CATG 3 cut(s) 365, 490, 512
NlaIV GGNNCC 2 cut(s) 256, 417
NmeAIII GCCGAG 1 cut(s) 793
NmuCI GTSAC 1 cut(s) 755
NspI RCATGY 2 cut(s) 365, 512
PciI ACATGT 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 535
PctI GAATGC 1 cut(s) 971
PdmI GAANNNNTTC 1 cut(s) 864
PfeI GAWTC 5 cut(s) 556, 665, 797, 814, 865
PflFI GACNNNGTC 1 cut(s) 41
PinAI ACCGGT 1 cut(s) 947
Ple19I CGATCG 1 cut(s) 231
PleI GAGTC 1 cut(s) 675
PmaCI CACGTG 1 cut(s) 539
PmlI CACGTG 1 cut(s) 539
PpsI GAGTC 1 cut(s) 675
Ppu21I YACGTR 1 cut(s) 539
PscI ACATGT 1 cut(s) 361
Psp1406I AACGTT 1 cut(s) 475
PspCI CACGTG 1 cut(s) 539
PspEI GGTNACC 1 cut(s) 775
PspN4I GGNNCC 2 cut(s) 256, 417
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 58
PsuI RGATCY 1 cut(s) 415
PsyI GACNNNGTC 1 cut(s) 41
PvuI CGATCG 1 cut(s) 231
PvuII CAGCTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 204, 734
Sau3AI GATC 2 cut(s) 228, 415
SchI GAGTC 1 cut(s) 675
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 3 cut(s) 148, 340, 445
SfcI CTRYAG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 733
SmoI CTYRAG 1 cut(s) 733
Sse9I AATT 2 cut(s) 74, 724
SsiI CCGC 5 cut(s) 163, 484, 587, 657, 879
SspMI CTAG 2 cut(s) 525, 584
StyI CCWWGG 2 cut(s) 327, 628
TaaI ACNGT 2 cut(s) 275, 347
TaiI ACGT 4 cut(s) 478, 541, 570, 845
TaqI TCGA 1 cut(s) 150
TasI AATT 2 cut(s) 74, 724
TauI GCSGC 2 cut(s) 486, 659
TfiI GAWTC 5 cut(s) 556, 665, 797, 814, 865
Tru1I TTAA 2 cut(s) 204, 734
Tru9I TTAA 2 cut(s) 204, 734
TscAI CASTG 2 cut(s) 85, 760
TseFI GTSAC 1 cut(s) 755
TseI GCWGC 8 cut(s) 52, 209, 332, 335, 647, 743, 881, 1040
Tsp45I GTSAC 1 cut(s) 755
TspDTI ATGAA 2 cut(s) 108, 857
TspGWI ACGGA 2 cut(s) 370, 530
TspRI CASTG 2 cut(s) 85, 760
Tth111I GACNNNGTC 1 cut(s) 41
Vha464I CTTAAG 1 cut(s) 733
XbaI TCTAGA 1 cut(s) 524
XceI RCATGY 2 cut(s) 365, 512
XmiI GTMKAC 1 cut(s) 366
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 2 cut(s) 525, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.