Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Reverse (-)

11160721 .. 11170071

Gene Structure 9351 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G20850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4143 bp

ATGGATCTGAGTCTGCAGCTGCAGATTCACGGCGCGTTAATCAACAAAGGCCCGTCGTGTACTTCTTATTGGGCTTCTTCTTCTTCACTTTCTCTTCCTCGCGATTTCATTTCTTCTTCTACGTTTCTTCTTCATCGAAGACTCAGAAGAAGAAGCTGCTCTCGTTCTCGTGGAATCCGATTAAGACGGAGTGGTTTCTCGGCGATGCCTTGTTCTTCTTCTGATACTCTTACGGCTTCTCGCGTCGGTTGTGGCGGAGGAGGTGGTGGTGGAGCCGTTGGCGGTGGAGCTGAGAATGCTTCTGTCGCTAATTTCAAGCTGAACGAATCTACCTTTATTGCTTCTCTCATGCCTAAGAAAGAAATCCGTGCGGATTGCTTCATCGAAGCTCATCCTGAGTACGACGGTCGTGGCGTTGTTATAGCTATCTTTGATTCTGGTTTTGATCCTTCTGCTGCTGGCTTGCATGTAACTTCTGATGGAAAACCCAAGGTTTTGGACGTCATTGACTGCACTGGAAGTGGAGATATAGATACATCCACAGTGGTGAAAGCCAATGAAGATGGTCATATTCGTGGAGCATCAGGGGCAACTTTGGTTGTCAATTCTTCATGGAAAAATCCCACTGGAGAGTGGCGTGTAGGTTCCAAGTTGGTTTATCAGTTATTTACAGATGACTTGACTTCTCGTGTGAAGAAAGAGAGAAGGAAATCCTGGGATGAAAAGAATCAGGAGGAAATTGCCAAGGCTGTAAATAATCTGTATGATTTCGATCAGAAGCACAGCAAGGTGGAAGATGCGAAATTGAAGAAAACTCGTGAAGACCTCCAAAGCAAAGTTGATTTTCTGAAAAAACAAGCTGATAAATATGAAGACAAAGGGCCTGTTATTGATGCTGTTGTGTGGCATGATGGAGAAGTATGGAGGGTTGCACTTGATACACAGAGTCTGGAAGAGGATCCAGATAGCGGGAAACTAGCAGACTTCTCCCCACTGACTAACTACCGAATTGAACGGAAGTATGGCGTGTTTAGCCGACTAGATGCATGCTCCTTTGTTGCTAACGTGTATGATGAGGGAAAGGTTCTAAGCATTGTAACTGATAGTTCTCCTCACGGCACTCATGTTGCTGGAATAGCTACTGCACACCACCCTGAGGAGCATTTGTTAAATGGTGTGGCACCTGGTGCACAAATAATATCTTGCAAAATTGGAGACTCGCGATTAGGTTCAATGGAGACCGGAACTGGATTGACTCGGGCATTGATAGCTGCCCTAGAGCATAACTGTGATCTTGTCAACATGAGCTATGGAGAGCCAGCATTACTTCCAGACTATGGGCGCTTTGTTGATCTTGTTACTGAAGCTGTGAACAAGAGACGTCTAATTTTTGTAAGTAGTGCTGGCAACAGTGGTCCAGCTTTGACAACAGTCGGAGCACCGGGTGGAACAACGTCCAGTATCATTGGGGTTGGTGCGTATGTTTCTCCTGCAATGGCAGCAGGTGCCCATTCAGTGGTGGAACCTCCCAGTGAGGGGCTTGAATACACTTGGTCAAGCCGGGGGCCAACATCTGATGGAGATCTTGGTGTCTGCATAAGTGCACCTGGTGGGGCTGTTGCTCCTGTTCCTACATGGACGCTTCAAAGACGCATGCTCATGAATGGAACATCAATGGCATCTCCTTCTGCCTGTGGGGCAATTGCGTTACTTCTAAGTGCTATGAAGGCTGAGGGCATCCCAGTTAGCCCGTACAGTGTGAGAAGGGCCCTTGAAAATACATCTACACCAGTAGGTGATCTACCTGAAGATAAACTAACTACTGGACAAGGGCTTATGCAAGTTGATAAGGCATATGAATACTTAAAACAGTTTCAGGACTATCCATGCGTATTTTATCAGATAAAGGTCAATCTCTCCGGTAAAACAATTCCAACATCACGAGGCATCTACCTGCGGGAGGGTACTGCTTGCAGACAGTCTACTGAGTGGACAATACAAGTTGATCCGAAATTCCATGAAGGTGCAAGTAATTTAAAGGAACTTGTCCCTTTCGAGGAATGTCTTGAGTTGCACTCCACAGATGAAGGAGTTGTACGGGTTCCTGACTATCTCCTTCTCACTAATAATGGACGTGGCTTCAACGTTGTTGTGGATCCCACAAATCTTGGGGATGGTGTGCATTACTTTGAAGTTTATGGGATTGATTGCAAAGCACCAGAGCGTGGTCCCCTTTTCAGAATCCCAGTAACAATAATAATTCCCAAGACTGTGGCAAATCAACCTCCAGTTATTTCATTTCAGCAAATGTCTTTCATCTCAGGCCACATTGAACGAAGATATATCGAGGTACCACATGGAGCAACATGGGCTGAGGCTACAATGCGAACTTCGGGGTTTGATACTACACGACGATTTTACATTGATACACTTCAGGTTTGCCCATTGCGAAGGCCTATCAAGTGGGAGAGCGCACCAACTTTCGCATCTCCGTCTGCTAAAAGCTTTGTGTTCCCTGTGGTTAGTGGTCAAACGATGGAACTAGCTATAGCTCAATTTTGGTCAAGTGGCCTCGGAAGTCGTGAACCAACTATCGTTGACTTTGAGATTGAATTCCATGGAGTTGGTGTCGATAAAGAAGAATTGTTACTTGATGGAAGTGAAGCACCAATAAAAGTTGAAGCTGAAGCACTATTGGCATCTGAAAAACTTGTTCCCATAGCTGTCCTGAACAAGATACGAGTTCCATATCAGCCTATTGATGCACAACTGAAGACTCTATCAACTGGCCGTGATAGACTACTGTCGGGCAAACAGATACTAGCATTGACCTTAACCTACAAGTTCAAATTAGAAGATTCAGCTGAAGTGAAACCGTATATACCATTATTGAACAATCGCATATATGACACAAAATTTGAGTCCCAGTTTTTTATGATCTCTGATACTAACAAGCGTGTATATGCAATGGGTGACGTTTATCCTGAGTCTTCAAAACTTCCCAAGGGAGAGTATAAACTTCAGCTGTATCTGAGGCATGAAAATGTGGAGCTGCTGGAAAAGTTGAAGCAGCTCACTGTGTTTATTGAAAGAAATATGGGGGAGATTCGGCTGAATTTACATTCTGAACCAGATGGTCCGTTCACTGGAAATGGTGCCTTTAAGTCCTCTGTTCTAATGCCAGGGGTGAAGGAAGCATTTTATCTGGGCCCACCAACAAAGGACAAGCTACCCAAGAATACCCCCCAAGGATCTATGTTGGTGGGAGAAATCTCATATGGGAAATTGTCATTTGATGAAAAAGAAGGGAAGAATCCAAAGGATAATCCGGTATCTTATCCGATATCATATGTGGTTCCACCAAATAAGCCTGAGGAAGATAAGAAGGCAGCTTCTGCCCCTACTTGCTCTAAATCAGTATCAGAACGCTTGGAACAAGAGGTTCGCGACACAAAGATCAAATTTCTTGGGAATCTAAAACAAGAAACTGAGGAAGAGCGTTCAGAGTGGAGAAAGTTGTGTACCTGTCTCAAGTCTGAATACCCAGACTATACTCCATTACTAGCTAAGATCCTGGAAGGTTTACTGTCTCGGTCTGATGCTGGGGACAAAATCAGCCATCACGAAGAGATTATAGAAGCTGCAAACGAAGTAGTACGCAGTGTTGACGTAGATGAGCTAGCAAGATTCTTGCTAGACAAAACTGAACCAGAAGATGATGAAGCAGAGAAATTGAAGAAAAAAATGGAGGTGACACGTGATCAACTGGCGGATGCACTATACCAGAAAGGGTTAGCCATGGCGAGAATCGAGAATCTGAAGGGTGAGAAGGAAGGTGAAGGAGAAGAAGAAAGTAGCCAGAAGGATAAATTCGAAGAGAATTTCAAAGAACTGACAAAATGGGTAGACGTGAAATCATCTAAATACGGTACTCTCACTGTTCTAAGAGAGAAACGGCTATCACGACTCGGGACCGCATTGAAGGTACTTGATGACTTGATCCAAAACGAGAACGAGACTGCGAATAAGAAACTCTACGAACTTAAACTGGATTTGCTGGAGGAAATAGGTTGGAGCCATTTGGTTACGTATGAGAAGCAATGGATGCAGGTTCGTTTCCCTAAAAGCCTACCCCTCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1380

Amino Acids

152.37

Weight (kDa)

5.77

Isoelectric Point (pI)

42.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	136 - 600	6.9e-47	Subtilase family
TPPII_Ig-like-1	PF21223	646 - 753	2e-24	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	774 - 856	1.2e-12	TPPII, galactose-binding domain-like
TPPII	PF12580	898 - 1081	4.7e-61	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1493
AatII	GACGTC	2 cut(s)	504, 1384
Acc36I	ACCTGC	3 cut(s)	1493, 1962, 4099
Acc65I	GGTACC	1 cut(s)	2350
AccB1I	GGYRCC	4 cut(s)	1180, 1505, 2350, 3155
AccB7I	CCANNNNNTGG	3 cut(s)	1337, 1516, 2225
AccI	GTMKAC	2 cut(s)	1982, 3906
AccII	CGCG	5 cut(s)	35, 102, 243, 1222, 3447
AciI	CCGC	7 cut(s)	255, 282, 371, 969, 1957, 3770, 3975
AclI	AACGTT	1 cut(s)	2145
AcoI	YGGCCR	1 cut(s)	2788
AcsI	RAATTY	7 cut(s)	2012, 2612, 2915, 3115, 3461, 3869, 3880
AcuI	CTGAAG	8 cut(s)	1383, 1827, 2417, 2706, 2792, 2886, 3005, 3839
AcvI	CACGTG	1 cut(s)	3758
AcyI	GRCGYC	2 cut(s)	501, 1381
AdeI	CACNNNGTG	3 cut(s)	1187, 1445, 1610
AflIII	ACRYGT	2 cut(s)	1065, 3755
AjiI	CACGTC	2 cut(s)	2135, 3910
AjnI	CCWGG	5 cut(s)	713, 1183, 1606, 3181, 3573
AleI	CACNNNNGTG	1 cut(s)	545
Alw21I	GWGCWC	3 cut(s)	1192, 1441, 1606
Alw26I	GTCTC	6 cut(s)	1209, 1232, 1372, 3533, 3594, 4010
Alw44I	GTGCAC	2 cut(s)	1188, 1602
AlwNI	CAGNNNCTG	2 cut(s)	949, 3395
Ama87I	CYCGRG	2 cut(s)	1257, 3968
AoxI	GGCC	9 cut(s)	49, 881, 1565, 1767, 2323, 2453, 2569, 2788, 3208
ApaI	GGGCCC	2 cut(s)	1771, 3212
ApaLI	GTGCAC	2 cut(s)	1188, 1602
ApoI	RAATTY	7 cut(s)	2012, 2612, 2915, 3115, 3461, 3869, 3880
ArsI	GACNNNNNNTTYG	4 cut(s)	2900, 2906, 2932, 2938
Asp718I	GGTACC	1 cut(s)	2350
AspLEI	GCGC	3 cut(s)	35, 1344, 2474
AsuC2I	CCSGG	2 cut(s)	1443, 1562
AsuHPI	GGTGA	7 cut(s)	559, 1808, 2984, 3199, 3763, 3836, 3848
AsuII	TTCGAA	1 cut(s)	3873
AsuNHI	GCTAGC	1 cut(s)	3679
AvaI	CYCGRG	2 cut(s)	1257, 3968
AvaII	GGWCC	4 cut(s)	1415, 2228, 3137, 3972
AxyI	CCTNAGG	2 cut(s)	1155, 3372
BaeGI	GKGCMC	5 cut(s)	1192, 1510, 1606, 1771, 3212
BamHI	GGATCC	2 cut(s)	958, 2155
BanI	GGYRCC	4 cut(s)	1180, 1505, 2350, 3155
BanII	GRGCYC	2 cut(s)	1771, 3212
BarI	GAAGNNNNNNTAC	4 cut(s)	2079, 2111, 2631, 2663
BauI	CACGAG	4 cut(s)	168, 687, 816, 1941
BbrPI	CACGTG	1 cut(s)	3758
BbsI	GAAGAC	5 cut(s)	145, 828, 879, 2780, 2982
Bbv12I	GWGCWC	3 cut(s)	1192, 1441, 1606
BbvCI	CCTCAGC	2 cut(s)	1731, 2373
BccI	CCATC	9 cut(s)	473, 557, 905, 1571, 2168, 2530, 2648, 3128, 3627
BceAI	ACGGC	6 cut(s)	46, 249, 260, 1132, 2775, 3971
BciT130I	CCWGG	5 cut(s)	715, 1185, 1608, 3183, 3575
BclI	TGATCA	1 cut(s)	3760
BcnI	CCSGG	2 cut(s)	1443, 1562
BcoDI	GTCTC	6 cut(s)	1209, 1232, 1372, 3533, 3594, 4010
BfaI	CTAG	8 cut(s)	977, 1040, 1277, 2543, 2822, 3563, 3680, 3695
BfmI	CTRYAG	3 cut(s)	14, 20, 2547
BfoI	RGCGCY	1 cut(s)	1345
BfuAI	ACCTGC	3 cut(s)	1493, 1962, 4099
BglI	GCCNNNNNGGC	1 cut(s)	1697
BglII	AGATCT	1 cut(s)	1582
Bme1390I	CCNGG	7 cut(s)	715, 1185, 1443, 1562, 1608, 3183, 3575
Bme18I	GGWCC	4 cut(s)	1415, 2228, 3137, 3972
BmeT110I	CYCGRG	2 cut(s)	1257, 3968
BmgBI	CACGTC	2 cut(s)	2135, 3910
BmrFI	CCNGG	7 cut(s)	715, 1185, 1443, 1562, 1608, 3183, 3575
BmrI	ACTGGG	4 cut(s)	1524, 1736, 2240, 2920
BmtI	GCTAGC	1 cut(s)	3683
BmuI	ACTGGG	4 cut(s)	1524, 1736, 2240, 2920
BoxI	GACNNNNGTC	2 cut(s)	1430, 2803
BpiI	GAAGAC	5 cut(s)	145, 828, 879, 2780, 2982
BplI	GAGNNNNNCTC	2 cut(s)	2060, 2092
BpmI	CTGGAG	3 cut(s)	648, 2271, 4079
Bpu10I	CCTNAGC	2 cut(s)	1731, 2373
Bpu14I	TTCGAA	1 cut(s)	3873
BpuEI	CTTGAG	2 cut(s)	2087, 3515
BpuMI	CCSGG	2 cut(s)	1443, 1562
BsaAI	YACGTR	2 cut(s)	3758, 4089
BsaBI	GATNNNNATC	2 cut(s)	771, 1581
BsaHI	GRCGYC	2 cut(s)	501, 1381
BsaI	GGTCTC	1 cut(s)	1232
BsaWI	WCCGGW	3 cut(s)	1241, 1919, 3328
BsaXI	ACNNNNNCTCC	4 cut(s)	1151, 1181, 2613, 2643
Bse21I	CCTNAGG	2 cut(s)	1155, 3372
Bse3DI	GCAATG	4 cut(s)	1500, 2444, 2973, 4106
Bse8I	GATNNNNATC	2 cut(s)	771, 1581
BseBI	CCWGG	5 cut(s)	715, 1185, 1608, 3183, 3575
BseGI	GGATG	7 cut(s)	391, 536, 724, 1737, 2179, 3778, 4110
BseJI	GATNNNNATC	2 cut(s)	771, 1581
BseMI	GCAATG	4 cut(s)	1500, 2444, 2973, 4106
BseRI	GAGGAG	3 cut(s)	273, 1101, 1172
BseSI	GKGCMC	5 cut(s)	1192, 1510, 1606, 1771, 3212
BseYI	CCCAGC	1 cut(s)	3602
BsgI	GTGCAG	2 cut(s)	496, 1128
Bsh1236I	CGCG	5 cut(s)	35, 102, 243, 1222, 3447
Bsh1285I	CGRYCG	1 cut(s)	409
BshFI	GGCC	9 cut(s)	51, 883, 1567, 1769, 2325, 2455, 2571, 2790, 3210
BshNI	GGYRCC	4 cut(s)	1180, 1505, 2350, 3155
BsiEI	CGRYCG	1 cut(s)	409
BsiHKAI	GWGCWC	3 cut(s)	1192, 1441, 1606
BsiHKCI	CYCGRG	2 cut(s)	1257, 3968
BsiSI	CCGG	5 cut(s)	1242, 1442, 1561, 1920, 3329
BslFI	GGGAC	5 cut(s)	2033, 2214, 2908, 3620, 3985
BsmAI	GTCTC	6 cut(s)	1209, 1232, 1372, 3533, 3594, 4010
BsmBI	CGTCTC	1 cut(s)	1372
BsmFI	GGGAC	5 cut(s)	2033, 2214, 2908, 3620, 3985
BsmI	GAATGC	1 cut(s)	301
BsnI	GGCC	9 cut(s)	51, 883, 1567, 1769, 2325, 2455, 2571, 2790, 3210
Bso31I	GGTCTC	1 cut(s)	1232
BsoBI	CYCGRG	2 cut(s)	1257, 3968
Bsp119I	TTCGAA	1 cut(s)	3873
Bsp120I	GGGCCC	2 cut(s)	1767, 3208
Bsp1286I	GDGCHC	6 cut(s)	1192, 1441, 1510, 1606, 1771, 3212
Bsp19I	CCATGG	2 cut(s)	2617, 3798
Bsp68I	TCGCGA	3 cut(s)	102, 1222, 3447
BspACI	CCGC	7 cut(s)	255, 282, 371, 969, 1957, 3770, 3975
BspANI	GGCC	9 cut(s)	51, 883, 1567, 1769, 2325, 2455, 2571, 2790, 3210
BspFNI	CGCG	5 cut(s)	35, 102, 243, 1222, 3447
BspHI	TCATGA	1 cut(s)	1659
BspMAI	CTGCAG	2 cut(s)	18, 24
BspMI	ACCTGC	3 cut(s)	1493, 1962, 4099
BspOI	GCTAGC	1 cut(s)	3683
BspQI	GCTCTTC	1 cut(s)	3489
BspT104I	TTCGAA	1 cut(s)	3873
BspT107I	GGYRCC	4 cut(s)	1180, 1505, 2350, 3155
BspTNI	GGTCTC	1 cut(s)	1232
BsrDI	GCAATG	4 cut(s)	1500, 2444, 2973, 4106
BssNI	GRCGYC	2 cut(s)	501, 1381
BssSI	CACGAG	4 cut(s)	168, 687, 816, 1941
BssT1I	CCWWGG	6 cut(s)	489, 744, 2617, 3003, 3247, 3798
Bst2BI	CACGAG	4 cut(s)	168, 687, 816, 1941
Bst2UI	CCWGG	5 cut(s)	715, 1185, 1608, 3183, 3575
Bst6I	CTCTTC	5 cut(s)	99, 948, 3489, 3621, 3870
BstACI	GRCGYC	2 cut(s)	501, 1381
BstAPI	GCANNNNNTGC	4 cut(s)	1187, 1505, 3395, 4105
BstBAI	YACGTR	2 cut(s)	3758, 4089
BstBI	TTCGAA	1 cut(s)	3873
BstC8I	GCNNGC	8 cut(s)	460, 464, 1048, 1320, 1405, 1655, 1972, 3681
BstDSI	CCRYGG	2 cut(s)	2617, 3798
BstENI	CCTNNNNNAGG	1 cut(s)	1958
BstF5I	GGATG	7 cut(s)	391, 536, 724, 1737, 2179, 3778, 4110
BstFNI	CGCG	5 cut(s)	35, 102, 243, 1222, 3447
BstH2I	RGCGCY	1 cut(s)	1345
BstHHI	GCGC	3 cut(s)	35, 1344, 2474
BstMAI	GTCTC	6 cut(s)	1209, 1232, 1372, 3533, 3594, 4010
BstMCI	CGRYCG	1 cut(s)	409
BstNI	CCWGG	5 cut(s)	715, 1185, 1608, 3183, 3575
BstNSI	RCATGY	3 cut(s)	470, 1050, 1657
BstPAI	GACNNNNGTC	2 cut(s)	1430, 2803
BstSCI	CCNGG	7 cut(s)	713, 1183, 1441, 1560, 1606, 3181, 3573
BstSFI	CTRYAG	3 cut(s)	14, 20, 2547
BstSLI	GKGCMC	5 cut(s)	1192, 1510, 1606, 1771, 3212
BstSNI	TACGTA	1 cut(s)	4089
BstUI	CGCG	5 cut(s)	35, 102, 243, 1222, 3447
BstV2I	GAAGAC	5 cut(s)	145, 828, 879, 2780, 2982
BstX2I	RGATCY	6 cut(s)	4, 958, 1582, 2155, 3251, 3570
BstXI	CCANNNNNNTGG	3 cut(s)	496, 2272, 2624
BstYI	RGATCY	6 cut(s)	4, 958, 1582, 2155, 3251, 3570
Bsu36I	CCTNAGG	2 cut(s)	1155, 3372
BsuRI	GGCC	9 cut(s)	51, 883, 1567, 1769, 2325, 2455, 2571, 2790, 3210
BtgI	CCRYGG	2 cut(s)	2617, 3798
BtgZI	GCGATG	1 cut(s)	218
BtrI	CACGTC	2 cut(s)	2135, 3910
BtsCI	GGATG	7 cut(s)	391, 536, 724, 1737, 2179, 3778, 4110
BtsI	GCAGTG	1 cut(s)	3667
BtuMI	TCGCGA	3 cut(s)	102, 1222, 3447
BveI	ACCTGC	3 cut(s)	1493, 1962, 4099
Cac8I	GCNNGC	8 cut(s)	460, 464, 1048, 1320, 1405, 1655, 1972, 3681
CaiI	CAGNNNCTG	2 cut(s)	949, 3395
CciI	TCATGA	1 cut(s)	1659
CfoI	GCGC	3 cut(s)	35, 1344, 2474
CseI	GACGC	3 cut(s)	232, 1648, 1659
CsiI	ACCWGGT	2 cut(s)	1183, 1606
CspCI	CAANNNNNGTGG	2 cut(s)	2149, 2184
DraI	TTTAAA	1 cut(s)	2037
DraIII	CACNNNGTG	3 cut(s)	1187, 1445, 1610
EaeI	YGGCCR	1 cut(s)	2788
Eam1104I	CTCTTC	5 cut(s)	99, 948, 3489, 3621, 3870
EarI	CTCTTC	5 cut(s)	99, 948, 3489, 3621, 3870
EciI	GGCGGA	2 cut(s)	270, 3785
Eco105I	TACGTA	1 cut(s)	4089
Eco130I	CCWWGG	6 cut(s)	489, 744, 2617, 3003, 3247, 3798
Eco147I	AGGCCT	1 cut(s)	2455
Eco24I	GRGCYC	2 cut(s)	1771, 3212
Eco31I	GGTCTC	1 cut(s)	1232
Eco32I	GATATC	1 cut(s)	3345
Eco47I	GGWCC	4 cut(s)	1415, 2228, 3137, 3972
Eco57I	CTGAAG	8 cut(s)	1383, 1827, 2417, 2706, 2792, 2886, 3005, 3839
Eco72I	CACGTG	1 cut(s)	3758
Eco81I	CCTNAGG	2 cut(s)	1155, 3372
Eco88I	CYCGRG	2 cut(s)	1257, 3968
EcoNI	CCTNNNNNAGG	1 cut(s)	1958
EcoO109I	RGGNCCY	3 cut(s)	881, 1767, 1768
EcoRI	GAATTC	1 cut(s)	2612
EcoRII	CCWGG	5 cut(s)	713, 1183, 1606, 3181, 3573
EcoRV	GATATC	1 cut(s)	3345
EcoT14I	CCWWGG	6 cut(s)	489, 744, 2617, 3003, 3247, 3798
EcoT22I	ATGCAT	1 cut(s)	1048
EcoT38I	GRGCYC	2 cut(s)	1771, 3212
ErhI	CCWWGG	6 cut(s)	489, 744, 2617, 3003, 3247, 3798
Esp3I	CGTCTC	1 cut(s)	1372
FaqI	GGGAC	5 cut(s)	2033, 2214, 2908, 3620, 3985
FauI	CCCGC	2 cut(s)	962, 1950
FauNDI	CATATG	3 cut(s)	1855, 3277, 3349
FbaI	TGATCA	1 cut(s)	3760
FblI	GTMKAC	2 cut(s)	1982, 3906
FokI	GGATG	7 cut(s)	378, 523, 731, 1724, 2186, 3785, 4117
FriOI	GRGCYC	2 cut(s)	1771, 3212
FspBI	CTAG	8 cut(s)	977, 1040, 1277, 2543, 2822, 3563, 3680, 3695
GlaI	GCGC	3 cut(s)	34, 1343, 2473
GsaI	CCCAGC	1 cut(s)	3606
GsuI	CTGGAG	3 cut(s)	648, 2271, 4079
HaeII	RGCGCY	1 cut(s)	1345
HaeIII	GGCC	9 cut(s)	51, 883, 1567, 1769, 2325, 2455, 2571, 2790, 3210
HapII	CCGG	5 cut(s)	1242, 1442, 1561, 1920, 3329
HgaI	GACGC	3 cut(s)	232, 1648, 1659
HhaI	GCGC	3 cut(s)	35, 1344, 2474
Hin1I	GRCGYC	2 cut(s)	501, 1381
Hin6I	GCGC	3 cut(s)	33, 1342, 2472
HinP1I	GCGC	3 cut(s)	33, 1342, 2472
HincII	GTYRAC	3 cut(s)	1300, 2599, 3667
HindII	GTYRAC	3 cut(s)	1300, 2599, 3667
HindIII	AAGCTT	1 cut(s)	2503
HpaII	CCGG	5 cut(s)	1242, 1442, 1561, 1920, 3329
HphI	GGTGA	7 cut(s)	559, 1808, 2984, 3199, 3763, 3836, 3848
Hpy99I	CGWCG	4 cut(s)	58, 248, 407, 2415
Hsp92I	GRCGYC	2 cut(s)	501, 1381
HspAI	GCGC	3 cut(s)	33, 1342, 2472
KpnI	GGTACC	1 cut(s)	2354
Ksp22I	TGATCA	1 cut(s)	3760
LguI	GCTCTTC	1 cut(s)	3489
MabI	ACCWGGT	2 cut(s)	1183, 1606
MaeI	CTAG	8 cut(s)	977, 1040, 1277, 2543, 2822, 3563, 3680, 3695
MaeIII	GTNAC	9 cut(s)	469, 1096, 1357, 1707, 2248, 2646, 2972, 3751, 4084
MfeI	CAATTG	1 cut(s)	1701
MflI	RGATCY	6 cut(s)	4, 958, 1582, 2155, 3251, 3570
MhlI	GDGCHC	6 cut(s)	1192, 1441, 1510, 1606, 1771, 3212
MlyI	GAGTC	9 cut(s)	19, 135, 955, 1211, 1249, 2770, 2930, 2996, 3960
MmeI	TCCRAC	3 cut(s)	1414, 1958, 4052
Mph1103I	ATGCAT	1 cut(s)	1048
MseI	TTAA	8 cut(s)	38, 182, 1169, 1865, 2036, 2834, 3162, 4044
MslI	CAYNNNNRTG	6 cut(s)	545, 573, 1287, 1658, 2022, 3042
MspA1I	CMGCKG	3 cut(s)	19, 2864, 3025
MspI	CCGG	5 cut(s)	1242, 1442, 1561, 1920, 3329
MspR9I	CCNGG	7 cut(s)	715, 1185, 1443, 1562, 1608, 3183, 3575
MunI	CAATTG	1 cut(s)	1701
Mva1269I	GAATGC	1 cut(s)	301
MvaI	CCWGG	5 cut(s)	715, 1185, 1608, 3183, 3575
MvnI	CGCG	5 cut(s)	35, 102, 243, 1222, 3447
NciI	CCSGG	2 cut(s)	1443, 1562
NcoI	CCATGG	2 cut(s)	2617, 3798
NdeI	CATATG	3 cut(s)	1855, 3277, 3349
NheI	GCTAGC	1 cut(s)	3679
NmeAIII	GCCGAG	1 cut(s)	179
NmuCI	GTSAC	2 cut(s)	2972, 3751
NruI	TCGCGA	3 cut(s)	102, 1222, 3447
NsiI	ATGCAT	1 cut(s)	1048
NspI	RCATGY	3 cut(s)	470, 1050, 1657
NspV	TTCGAA	1 cut(s)	3873
OliI	CACNNNNGTG	1 cut(s)	545
PaeI	GCATGC	2 cut(s)	1050, 1657
PagI	TCATGA	1 cut(s)	1659
PaqCI	CACCTGC	1 cut(s)	1493
PceI	AGGCCT	1 cut(s)	2455
PciSI	GCTCTTC	1 cut(s)	3489
PcsI	WCGNNNNNNNCGW	3 cut(s)	175, 411, 3961
PctI	GAATGC	1 cut(s)	301
PflMI	CCANNNNNTGG	3 cut(s)	1337, 1516, 2225
PfoI	TCCNGGA	1 cut(s)	3573
PleI	GAGTC	9 cut(s)	18, 135, 954, 1211, 1249, 2770, 2929, 2995, 3960
PmaCI	CACGTG	1 cut(s)	3758
PmlI	CACGTG	1 cut(s)	3758
PpsI	GAGTC	9 cut(s)	18, 135, 954, 1211, 1249, 2770, 2929, 2995, 3960
Ppu21I	YACGTR	2 cut(s)	3758, 4089
PshAI	GACNNNNGTC	2 cut(s)	1430, 2803
Psp1406I	AACGTT	1 cut(s)	2145
Psp6I	CCWGG	5 cut(s)	713, 1183, 1606, 3181, 3573
PspCI	CACGTG	1 cut(s)	3758
PspFI	CCCAGC	1 cut(s)	3602
PspGI	CCWGG	5 cut(s)	713, 1183, 1606, 3181, 3573
PspOMI	GGGCCC	2 cut(s)	1767, 3208
PstI	CTGCAG	2 cut(s)	18, 24
PstNI	CAGNNNCTG	2 cut(s)	949, 3395
PsuI	RGATCY	6 cut(s)	4, 958, 1582, 2155, 3251, 3570
PvuII	CAGCTG	3 cut(s)	19, 2864, 3025
RruI	TCGCGA	3 cut(s)	102, 1222, 3447
RseI	CAYNNNNRTG	6 cut(s)	545, 573, 1287, 1658, 2022, 3042
SapI	GCTCTTC	1 cut(s)	3489
SaqAI	TTAA	8 cut(s)	38, 182, 1169, 1865, 2036, 2834, 3162, 4044
SchI	GAGTC	9 cut(s)	19, 135, 955, 1211, 1249, 2770, 2930, 2996, 3960
ScrFI	CCNGG	7 cut(s)	715, 1185, 1443, 1562, 1608, 3183, 3575
SduI	GDGCHC	6 cut(s)	1192, 1441, 1510, 1606, 1771, 3212
SexAI	ACCWGGT	2 cut(s)	1183, 1606
SfcI	CTRYAG	3 cut(s)	14, 20, 2547
SfuI	TTCGAA	1 cut(s)	3873
SinI	GGWCC	4 cut(s)	1415, 2228, 3137, 3972
SmiMI	CAYNNNNRTG	6 cut(s)	545, 573, 1287, 1658, 2022, 3042
SmlI	CTYRAG	2 cut(s)	2066, 3530
SmoI	CTYRAG	2 cut(s)	2066, 3530
SnaBI	TACGTA	1 cut(s)	4089
SphI	GCATGC	2 cut(s)	1050, 1657
SseBI	AGGCCT	1 cut(s)	2455
SsiI	CCGC	7 cut(s)	255, 282, 371, 969, 1957, 3770, 3975
SspMI	CTAG	8 cut(s)	977, 1040, 1277, 2543, 2822, 3563, 3680, 3695
StuI	AGGCCT	1 cut(s)	2455
StyD4I	CCNGG	7 cut(s)	713, 1183, 1441, 1560, 1606, 3181, 3573
StyI	CCWWGG	6 cut(s)	489, 744, 2617, 3003, 3247, 3798
TaqI	TCGA	8 cut(s)	136, 384, 771, 2055, 2346, 2631, 3810, 3873
TaqII	GACCGA	1 cut(s)	3582
TatI	WGTACW	1 cut(s)	59
Tru1I	TTAA	8 cut(s)	38, 182, 1169, 1865, 2036, 2834, 3162, 4044
Tru9I	TTAA	8 cut(s)	38, 182, 1169, 1865, 2036, 2834, 3162, 4044
TseFI	GTSAC	2 cut(s)	2972, 3751
Tsp45I	GTSAC	2 cut(s)	2972, 3751
TspGWI	ACGGA	5 cut(s)	202, 356, 1030, 2481, 3129
Van91I	CCANNNNNTGG	3 cut(s)	1337, 1516, 2225
VneI	GTGCAC	2 cut(s)	1188, 1602
VpaK11BI	GGWCC	4 cut(s)	1415, 2228, 3137, 3972
XagI	CCTNNNNNAGG	1 cut(s)	1958
XapI	RAATTY	7 cut(s)	2012, 2612, 2915, 3115, 3461, 3869, 3880
XceI	RCATGY	3 cut(s)	470, 1050, 1657
XmiI	GTMKAC	2 cut(s)	1982, 3906
XspI	CTAG	8 cut(s)	977, 1040, 1277, 2543, 2822, 3563, 3680, 3695
ZraI	GACGTC	2 cut(s)	502, 1382
Zsp2I	ATGCAT	1 cut(s)	1048

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.