Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Forward (+)

65614254 .. 65626707

Gene Structure 12454 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG428700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4125 bp

ATGCAAAACGCCGTCGTCTTATTCAAATTACCTTATTTGCCACCGTCACGTCTCCTCCCACTTCATTATCACCATCACTCGCTAATCATTAGACCCAAAAAACCGAGCAGAGAGAGACGAAGACCAAGAGAGACCGAGTGGAGTGATCGCAGTAGAACCAGTAGCGTTAGGATTAGGGCAATGCCTTGCTCTGCGATTAGCGGCGGCGGTAGCGACGGTAATGGTTCTCTGCGTAATTTCAAGCTCAACGAGTCCACGTTCTTAGCCTCGCTCATGCCGAAGAAGGAGATCGGCGCCGATCGATTCGTCGAGGCTCATCCGAATTACGACGGCCGCGGCGTCGTTATTGCTATCTTCGATTCTGGAGTGGACCCGGCTGCTGCTGGATTACAGGTTACGTCAGATGGAAAGCCGAAAATTCTAGATGTTCTTGACTGCACTGGGAGTGGAGATGTTGATACTTCAAAGGTGGTGAAGGCCGATGAGAACGGTTGCATACCTGGGGCTTCAGGGGCATCTCTGCACGTCAACTCTTCATGGAAAAATCCTTCTGGTGAATGGCATGTGGGTTATAAGTTGGTTTACGAGCTATTTACTGGCACATTGACTTCCCGCTTGAAGAGAGAAAGAAGGAAAAAATGGGACGAACAAAACCAAGAGGAAATTGCTAAGGCTGTTAAGCATCTTCATGAATTTGACCAGAAACACATTAGAGCGGAGGATGCTAACTTGAAAAGGGCTCGGGAGGACCTGCAAAACAGAGTTGATTACCTACAAAAGCAAGCTGAAAGCTATGATGACAAAGGGCCTGTCATAGATGCTGTTGTATGGCATGATGGAGAAGTATGGAGGGTTGCCATTGACACACGGACTCTTGAGGATGACCCTGATTGCGGAAAACTCGCTGACTTTGTGCCCCTAACTAATTATAGGATAGAACGAAAGTATGGCGTTTTCAGCAAATTGGATGCCTGTACGTTTGTTGTTAATGTGTATGATGAAGGGAAGATATTGAGTATTGTAACAGATTGCTCTCCCCATGGCACACATGTTGCTGGTATTGCTACTGCTTTCCATGCGAAGGAGCCCATGTTGAATGGAGTTGCACCTGGAGCACAAGTTATATCTTGTAAAATTGGGGATGCACGCTTAGGTTCAATGGAGACAGGAACTGGGCTGACTCGAGCTTTGATTGCTGCTGTGGAGCATAAATGTGATCTGATCAACATGAGCTATGGAGAACCGGCATTACTGCCAAACTATGGGCGCTTTGTTGACCTCGTTAATGAAGCTGTGAACAAGCACCGTCTGGTATTTGTGAGTAGTGCCGGCAATAGTGGGCCGGCATTGAGCACTGTTGGGGCACCTGGTGGTACCACATCAAGCATTATAGGAGTTGGTGCATATGTATCTCCTGCAATGGCTGCTGGTGCTCATTGTGTAGTTGAAGCCCCCGGTGAAGGTCTTGAATACACTTGGTCTAGCCGAGGACCAGCTGCCGATGGAGATCTTGGTGTCTGTATTAGTGCTCCTGGTGCAGCTATTGCTCCTGTTCCTACGTGGACCCTCCAACGACGGATGCTCATGAATGGAACATCAATGGCATCCCCATCTGCTTGTGGTGGAATTGCACTACTCATAAGTGCATTGAAGGCTGAGGGTATTTCTGTGAGTCCATATAGTGTAAGGAAGGCTCTTGAAAATACATCTGTTCCTGTAGGCAGTTTGCCAGAAGATAAATTATCAACTGGGCAAGGACTGATGCAAGTTGACAAGGCACTTGAATACCTAAAGCAGTCTCGGGATGTACCGTCTGTCTGGTATCAGATAAAGATAAACCAATCTGGTAAAACAACTCCTACATCACGAGGTATTTACTTAAGGGAGGCTAGTGCTTGCCAACAATCTACCGAGTGGACAGTGCAAGTTGAACCAAAATTTCATGAGGGTGCGAGTAATTTAGAAGAATTGGTTCCTTTTGAGGAGTGTATTGAGCTATGTTCCACTGACAATGCAGTTGTGAGAGCTCCTGAGTTTCTCCTTCTTACTCATAATGGCCGTAGCTTCAACATAGTTGTAAATCCTACCAACCTTAGTGAAGGTCTGCACTATTATGAACTATATGGTGTTGATTGCAAAGCACCTTGGCGCGGCCCTCTTTTCAGAATCCCAATTACTATAACAAAGCCAATCACTGTCATAAGTCGACCTCCACTATATTCATTTTCGAGGTTGTCTTTTTTGCCAGGCCACATAGAAAGGAGATTTATTGAAGTACCTCTTGGTGCCACATGGGTTGAAGCAACCATGCAAACATCAGGATTTGATACAACACGAAGATTTTTTGTTGACTCTGTTCAGCTTTGTCCCTTACAAAGACCTCGTAAGTGGGAGAGTGTTGTAACATTCTCTTCGCCTGCTGCCAAAAGCTTTTCATTTCCTGTTGTGGGTGGTCAGACAATGGAATTAGCTATCGCTCAGTTTTGGTCAAGTGGCATAGGAAGCAACGAAACAACTTGTGTGGATTTTGAGATTGTGTTTCATGGGATTAATGTTAATAAAGAGGAACTAGTGCTTGATGGAAGTGAGGCACCAGTAAGAATTGAAGCTGAAGCTCTACTAGCATCCGAGAAACTTGCACCTGCTGCTACTCTAAGCAAGATCAGAATTCCATATCGACCAGTTGCGGCTGAACTTTGTTCTCTTCCAACTGATCGTGACAAACTACCCTCAGAAAAACGTATTTTGGCGCTGACATTAACTTACAAGTTCAAATTGGAAGATGGAGCTGAAGTAAAACCTCAAGTTCCATTACTTAACGATCGCATATATGATACTAAATTTGAGTCTCAGTTTTATATGATCTCTGATGCAAACAAGCGTGTATATGCAACGGGTGAAGCTTATCCAAGCTCTTCAAAACTGCCCAAAGGCGAATACACTTTACAGCTATATCTTAGGCACGAAAACATGCAATATCTGGAAAAATTGAAGCAGCTGGTTTTGTTCATTGAAAGAAAATTGGAAGAGAAGGATGTAATGCGTTTGAGTTTTTTCTCTCAACCGGATGGCCCTGTGATGGGAAATGGTGCTTATAAGTCCTCAGTTTTAGTTCCAGGGAAAAAAGAAGCAATCTATTTGGGCCCACCATCCAAAGACAAGCTCCTAAAGTTTTCTCCGCAAGGATCTGTTTTGCTTGGAGCTATCTCCTATGGGAAGCTATCATATGCTGAAAAAGGACAAGGGAAAGATCCGCAAAAGAACCCGGTGTCTTATCAGATTTCTTATATTGTGCCACCGAATAAGATGGATGAAGACAAAGGAAAAGGTTCTTCTGCTTCCACCAAAGCTGTTTCTGAACGTTTAGAAGAAGAGGTTCGAGATGCGAAGATTAAAGTTCTTACAAGCCTCAAACAAGACAATGAAGAAGAACGCTCAGAGTGGAACAAATTATCTACCTCTCTTAAGTCTGAATACCCAAAACACACCCCATTGCTTGCAAAGATTTTGGAAGGTTTGCTTTCCCGAAACAGTGTCGAGGACAAAATCTGTCATGACAAAGAGGTTATTGATGCAGCAAATGAGGTTGTTGATAGTATTGACAGAGATGAGCTAGCGAAATTTTTTTCACTTAGAACTGATCCTGAAGATGAGGAGGCAGAGAAAAATAAAAAGAAGATGGAGACGACTCGTGATCAGTTAGCAGAGGCATTGTACCAAAAAGGACTAGCACTGGCAGATATTTTGTCTTTACAGGGGGATAAGCCTTCGGCGTCGGAAGTAACTGAAGGTTCAAAAGAGGCAGACAAGACTGCTGACTCTGGACCAGGCTCTGGTGGGCTGCTTGACCGCTTTGAAGACACTTTTAAAGAACTTCAAAAATGGGTCGAAGTGAAATCCTCTAAATACGGAATCCTCTCAGTAACACGTGAGAAACATCATGGAAGGCTTGGAACAGCACTCAAGGTCTTGAATGACATAATTCAAGAGAATACGGAGCCTCCGAAGAAGAAGCTTTACGAAGAAAAGCTGTCTCTGCTTGAAGAGATCGGGTGGCAGCACTTGGTGACATATGAGAGACAATGGATGCATGTGCGCTTTCCACCAAGTTTACCTCTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1374

Amino Acids

151.81

Weight (kDa)

6.36

Isoelectric Point (pI)

41.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	111 - 574	2.5e-44	Subtilase family
TPPII_Ig-like-1	PF21223	621 - 727	1e-22	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	747 - 831	2.1e-15	TPPII, galactose-binding domain-like
TPPII	PF12580	873 - 1057	1.2e-59	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	573, 3096
AarI	CACCTGC	1 cut(s)	2650
Acc36I	ACCTGC	2 cut(s)	759, 2650
Acc65I	GGTACC	1 cut(s)	1373
AccB1I	GGYRCC	5 cut(s)	293, 1363, 1373, 2285, 2590
AccB7I	CCANNNNNTGG	2 cut(s)	1262, 3833
AccBSI	CCGCTC	1 cut(s)	716
AccI	GTMKAC	1 cut(s)	2206
AccII	CGCG	2 cut(s)	336, 2151
AclI	AACGTT	1 cut(s)	3361
AclWI	GGATC	3 cut(s)	3193, 3245, 3635
AcoI	YGGCCR	2 cut(s)	331, 2056
AcsI	RAATTY	6 cut(s)	417, 692, 1937, 2667, 2840, 3620
AcuI	CTGAAG	5 cut(s)	492, 2631, 2811, 3666, 3807
AcvI	CACGTG	1 cut(s)	3929
AcyI	GRCGYC	3 cut(s)	294, 339, 3773
AdeI	CACNNNGTG	2 cut(s)	1370, 4066
AfaI	GTAC	5 cut(s)	976, 1375, 1809, 2277, 3716
AfiI	CCNNNNNNNGG	9 cut(s)	893, 1081, 1262, 1460, 2150, 2447, 3079, 3080, 3833
AflII	CTTAAG	2 cut(s)	1879, 3464
AflIII	ACRYGT	2 cut(s)	1048, 3926
AhlI	ACTAGT	1 cut(s)	2569
AjiI	CACGTC	2 cut(s)	50, 526
AjnI	CCWGG	7 cut(s)	499, 1108, 1366, 1531, 2245, 3115, 3826
AjuI	GAANNNNNNNTTGG	2 cut(s)	2265, 2297
Alw21I	GWGCWC	5 cut(s)	1117, 1355, 1435, 1531, 2029
Alw26I	GTCTC	9 cut(s)	56, 109, 125, 1157, 1803, 2853, 3677, 4038, 4072
AlwI	GGATC	3 cut(s)	3193, 3245, 3635
AlwNI	CAGNNNCTG	2 cut(s)	1172, 3833
Ama87I	CYCGRG	3 cut(s)	741, 1182, 1800
AoxI	GGCC	9 cut(s)	331, 477, 806, 1340, 2056, 2152, 2248, 3070, 3142
ApaI	GGGCCC	1 cut(s)	3146
ApoI	RAATTY	6 cut(s)	417, 692, 1937, 2667, 2840, 3620
ArsI	GACNNNNNNTTYG	4 cut(s)	795, 827, 1171, 1203
AseI	ATTAAT	1 cut(s)	2550
Asp700I	GAANNNNTTC	3 cut(s)	1971, 3328, 3375
Asp718I	GGTACC	1 cut(s)	1373
AspLEI	GCGC	5 cut(s)	296, 1269, 2151, 2752, 4098
AsuC2I	CCSGG	3 cut(s)	374, 1455, 3266
AsuHPI	GGTGA	6 cut(s)	62, 484, 566, 1469, 2909, 4078
AsuNHI	GCTAGC	1 cut(s)	3613
AvaI	CYCGRG	3 cut(s)	741, 1182, 1800
AvaII	GGWCC	5 cut(s)	370, 748, 1490, 1563, 3824
BaeGI	GKGCMC	3 cut(s)	918, 1366, 3146
BanI	GGYRCC	5 cut(s)	293, 1363, 1373, 2285, 2590
BanII	GRGCYC	4 cut(s)	742, 1089, 2029, 3146
BarI	GAAGNNNNNNTAC	2 cut(s)	4001, 4033
BauI	CACGAG	2 cut(s)	1866, 3690
BbrPI	CACGTG	1 cut(s)	3929
BbsI	GAAGAC	3 cut(s)	127, 3321, 3864
Bbv12I	GWGCWC	5 cut(s)	1117, 1355, 1435, 1531, 2029
BbvCI	CCTCAGC	1 cut(s)	1656
BceAI	ACGGC	2 cut(s)	346, 2043
BcgI	CGANNNNNNTGC	2 cut(s)	2618, 2652
BciT130I	CCWGG	7 cut(s)	501, 1110, 1368, 1533, 2247, 3117, 3828
BclI	TGATCA	2 cut(s)	1221, 3694
BcnI	CCSGG	3 cut(s)	374, 1455, 3266
BcoDI	GTCTC	9 cut(s)	56, 109, 125, 1157, 1803, 2853, 3677, 4038, 4072
BcuI	ACTAGT	1 cut(s)	2569
BfaI	CTAG	7 cut(s)	422, 1482, 1890, 2570, 2621, 3614, 3728
BfmI	CTRYAG	1 cut(s)	1716
BfoI	RGCGCY	3 cut(s)	297, 1270, 2753
BfrI	CTTAAG	2 cut(s)	1879, 3464
BfuAI	ACCTGC	2 cut(s)	759, 2650
BglII	AGATCT	1 cut(s)	1507
Bme18I	GGWCC	5 cut(s)	370, 748, 1490, 1563, 3824
BmeT110I	CYCGRG	3 cut(s)	741, 1182, 1800
BmgBI	CACGTC	2 cut(s)	50, 526
BmrI	ACTGGG	3 cut(s)	450, 1182, 1758
BmtI	GCTAGC	1 cut(s)	3617
BmuI	ACTGGG	3 cut(s)	450, 1182, 1758
BpiI	GAAGAC	3 cut(s)	127, 3321, 3864
BpmI	CTGGAG	2 cut(s)	384, 1131
Bpu10I	CCTNAGC	3 cut(s)	669, 1150, 1656
BpuEI	CTTGAG	3 cut(s)	896, 2787, 3947
BpuMI	CCSGG	3 cut(s)	374, 1455, 3266
Bsa29I	ATCGAT	1 cut(s)	301
BsaAI	YACGTR	2 cut(s)	1560, 3929
BsaBI	GATNNNNATC	1 cut(s)	1506
BsaHI	GRCGYC	3 cut(s)	294, 339, 3773
BsaI	GGTCTC	1 cut(s)	125
BsaJI	CCNNGG	7 cut(s)	334, 500, 1039, 1453, 1486, 2144, 3116
BsaWI	WCCGGW	1 cut(s)	3064
Bsc4I	CCNNNNNNNGG	9 cut(s)	893, 1081, 1262, 1460, 2150, 2447, 3079, 3080, 3833
Bse118I	RCCGGY	3 cut(s)	1243, 1328, 1342
Bse1I	ACTGG	8 cut(s)	159, 445, 601, 1177, 1753, 2594, 2681, 3738
Bse3DI	GCAATG	3 cut(s)	186, 1425, 3491
Bse8I	GATNNNNATC	1 cut(s)	1506
BseBI	CCWGG	7 cut(s)	501, 1110, 1368, 1533, 2247, 3117, 3828
BseCI	ATCGAT	1 cut(s)	301
BseDI	CCNNGG	7 cut(s)	334, 500, 1039, 1453, 1486, 2144, 3116
BseJI	GATNNNNATC	1 cut(s)	1506
BseLI	CCNNNNNNNGG	9 cut(s)	893, 1081, 1262, 1460, 2150, 2447, 3079, 3080, 3833
BseMI	GCAATG	3 cut(s)	186, 1425, 3491
BseMII	CTCAG	8 cut(s)	1647, 2022, 2492, 2745, 2864, 3117, 3450, 3933
BseNI	ACTGG	8 cut(s)	159, 445, 601, 1177, 1753, 2594, 2681, 3738
BseRI	GAGGAG	3 cut(s)	44, 1997, 3668
BseSI	GKGCMC	3 cut(s)	918, 1366, 3146
BseX3I	CGGCCG	1 cut(s)	331
BsgI	GTGCAG	4 cut(s)	421, 506, 1557, 2090
Bsh1236I	CGCG	2 cut(s)	336, 2151
Bsh1285I	CGRYCG	3 cut(s)	301, 334, 2824
BshFI	GGCC	9 cut(s)	333, 479, 808, 1342, 2058, 2154, 2250, 3072, 3144
BshNI	GGYRCC	5 cut(s)	293, 1363, 1373, 2285, 2590
BshVI	ATCGAT	1 cut(s)	301
BsiEI	CGRYCG	3 cut(s)	301, 334, 2824
BsiHKAI	GWGCWC	5 cut(s)	1117, 1355, 1435, 1531, 2029
BsiHKCI	CYCGRG	3 cut(s)	741, 1182, 1800
BsiSI	CCGG	7 cut(s)	374, 1244, 1329, 1343, 1455, 3065, 3266
BslFI	GGGAC	2 cut(s)	656, 2352
BslI	CCNNNNNNNGG	9 cut(s)	893, 1081, 1262, 1460, 2150, 2447, 3079, 3080, 3833
BsmAI	GTCTC	9 cut(s)	56, 109, 125, 1157, 1803, 2853, 3677, 4038, 4072
BsmBI	CGTCTC	3 cut(s)	56, 109, 3677
BsmFI	GGGAC	2 cut(s)	656, 2352
BsnI	GGCC	9 cut(s)	333, 479, 808, 1342, 2058, 2154, 2250, 3072, 3144
Bso31I	GGTCTC	1 cut(s)	125
BsoBI	CYCGRG	3 cut(s)	741, 1182, 1800
Bsp120I	GGGCCC	1 cut(s)	3142
Bsp19I	CCATGG	1 cut(s)	1039
BspANI	GGCC	9 cut(s)	333, 479, 808, 1342, 2058, 2154, 2250, 3072, 3144
BspCNI	CTCAG	8 cut(s)	1648, 2023, 2491, 2744, 2863, 3116, 3449, 3932
BspDI	ATCGAT	1 cut(s)	301
BspFNI	CGCG	2 cut(s)	336, 2151
BspHI	TCATGA	4 cut(s)	688, 1584, 1942, 3553
BspMI	ACCTGC	2 cut(s)	759, 2650
BspOI	GCTAGC	1 cut(s)	3617
BspPI	GGATC	3 cut(s)	3193, 3245, 3635
BspQI	GCTCTTC	1 cut(s)	2920
BspT107I	GGYRCC	5 cut(s)	293, 1363, 1373, 2285, 2590
BspTI	CTTAAG	2 cut(s)	1879, 3464
BspTNI	GGTCTC	1 cut(s)	125
BsrBI	CCGCTC	1 cut(s)	716
BsrDI	GCAATG	3 cut(s)	186, 1425, 3491
BsrFI	RCCGGY	3 cut(s)	1243, 1328, 1342
BsrI	ACTGG	8 cut(s)	159, 445, 601, 1177, 1753, 2594, 2681, 3738
BssAI	RCCGGY	3 cut(s)	1243, 1328, 1342
BssECI	CCNNGG	7 cut(s)	334, 500, 1039, 1453, 1486, 2144, 3116
BssNI	GRCGYC	3 cut(s)	294, 339, 3773
BssSI	CACGAG	2 cut(s)	1866, 3690
BssT1I	CCWWGG	2 cut(s)	1039, 2144
Bst2BI	CACGAG	2 cut(s)	1866, 3690
Bst2UI	CCWGG	7 cut(s)	501, 1110, 1368, 1533, 2247, 3117, 3828
Bst4CI	ACNGT	9 cut(s)	45, 218, 491, 1307, 1357, 1812, 1921, 2197, 3533
Bst6I	CTCTTC	8 cut(s)	538, 614, 2416, 2709, 2920, 3021, 3366, 4038
BstACI	GRCGYC	3 cut(s)	294, 339, 3773
BstAFI	CTTAAG	2 cut(s)	1879, 3464
BstAPI	GCANNNNNTGC	3 cut(s)	1424, 1544, 2645
BstBAI	YACGTR	2 cut(s)	1560, 3929
BstC8I	GCNNGC	8 cut(s)	783, 1147, 1330, 1344, 1897, 2418, 3498, 3615
BstDSI	CCRYGG	2 cut(s)	334, 1039
BstFNI	CGCG	2 cut(s)	336, 2151
BstH2I	RGCGCY	3 cut(s)	297, 1270, 2753
BstHHI	GCGC	5 cut(s)	296, 1269, 2151, 2752, 4098
BstMAI	GTCTC	9 cut(s)	56, 109, 125, 1157, 1803, 2853, 3677, 4038, 4072
BstMCI	CGRYCG	3 cut(s)	301, 334, 2824
BstNI	CCWGG	7 cut(s)	501, 1110, 1368, 1533, 2247, 3117, 3828
BstNSI	RCATGY	4 cut(s)	566, 1052, 2974, 4094
BstSFI	CTRYAG	1 cut(s)	1716
BstSLI	GKGCMC	3 cut(s)	918, 1366, 3146
BstUI	CGCG	2 cut(s)	336, 2151
BstV2I	GAAGAC	3 cut(s)	127, 3321, 3864
BstX2I	RGATCY	3 cut(s)	1507, 3185, 3250
BstYI	RGATCY	3 cut(s)	1507, 3185, 3250
BstZI	CGGCCG	1 cut(s)	331
Bsu15I	ATCGAT	1 cut(s)	301
BsuRI	GGCC	9 cut(s)	333, 479, 808, 1342, 2058, 2154, 2250, 3072, 3144
BsuTUI	ATCGAT	1 cut(s)	301
BtgI	CCRYGG	2 cut(s)	334, 1039
BtrI	CACGTC	2 cut(s)	50, 526
BtsIMutI	CAGTG	7 cut(s)	438, 1353, 1926, 2004, 2193, 3538, 3731
BveI	ACCTGC	2 cut(s)	759, 2650
Cac8I	GCNNGC	8 cut(s)	783, 1147, 1330, 1344, 1897, 2418, 3498, 3615
CaiI	CAGNNNCTG	2 cut(s)	1172, 3833
CciI	TCATGA	4 cut(s)	688, 1584, 1942, 3553
CfoI	GCGC	5 cut(s)	296, 1269, 2151, 2752, 4098
Cfr10I	RCCGGY	3 cut(s)	1243, 1328, 1342
Cfr42I	CCGCGG	1 cut(s)	337
ClaI	ATCGAT	1 cut(s)	301
CseI	GACGC	2 cut(s)	328, 3762
CsiI	ACCWGGT	1 cut(s)	1366
Csp6I	GTAC	5 cut(s)	975, 1374, 1808, 2276, 3715
CspCI	CAANNNNNGTGG	4 cut(s)	2278, 2313, 2502, 2537
CviQI	GTAC	5 cut(s)	975, 1374, 1808, 2276, 3715
DinI	GGCGCC	1 cut(s)	295
DraI	TTTAAA	1 cut(s)	3868
DraIII	CACNNNGTG	2 cut(s)	1370, 4066
EaeI	YGGCCR	2 cut(s)	331, 2056
EagI	CGGCCG	1 cut(s)	331
Eam1104I	CTCTTC	8 cut(s)	538, 614, 2416, 2709, 2920, 3021, 3366, 4038
EarI	CTCTTC	8 cut(s)	538, 614, 2416, 2709, 2920, 3021, 3366, 4038
Ecl136II	GAGCTC	1 cut(s)	2027
EclXI	CGGCCG	1 cut(s)	331
Eco130I	CCWWGG	2 cut(s)	1039, 2144
Eco24I	GRGCYC	4 cut(s)	742, 1089, 2029, 3146
Eco31I	GGTCTC	1 cut(s)	125
Eco47I	GGWCC	5 cut(s)	370, 748, 1490, 1563, 3824
Eco52I	CGGCCG	1 cut(s)	331
Eco53kI	GAGCTC	1 cut(s)	2027
Eco57I	CTGAAG	5 cut(s)	492, 2631, 2811, 3666, 3807
Eco72I	CACGTG	1 cut(s)	3929
Eco88I	CYCGRG	3 cut(s)	741, 1182, 1800
EcoICRI	GAGCTC	1 cut(s)	2027
EcoO109I	RGGNCCY	2 cut(s)	748, 806
EcoRI	GAATTC	1 cut(s)	2667
EcoRII	CCWGG	7 cut(s)	499, 1108, 1366, 1531, 2245, 3115, 3826
EcoT14I	CCWWGG	2 cut(s)	1039, 2144
EcoT22I	ATGCAT	1 cut(s)	4092
EcoT38I	GRGCYC	4 cut(s)	742, 1089, 2029, 3146
EgeI	GGCGCC	1 cut(s)	295
EheI	GGCGCC	1 cut(s)	295
ErhI	CCWWGG	2 cut(s)	1039, 2144
Esp3I	CGTCTC	3 cut(s)	56, 109, 3677
FalI	AAGNNNNNCTT	4 cut(s)	2265, 2297, 3504, 3536
FaqI	GGGAC	2 cut(s)	656, 2352
FauI	CCCGC	1 cut(s)	620
FauNDI	CATATG	3 cut(s)	1405, 3226, 4072
FbaI	TGATCA	2 cut(s)	1221, 3694
FblI	GTMKAC	1 cut(s)	2206
FriOI	GRGCYC	4 cut(s)	742, 1089, 2029, 3146
FspBI	CTAG	7 cut(s)	422, 1482, 1890, 2570, 2621, 3614, 3728
GlaI	GCGC	5 cut(s)	295, 1268, 2150, 2751, 4097
GsuI	CTGGAG	2 cut(s)	384, 1131
HaeII	RGCGCY	3 cut(s)	297, 1270, 2753
HaeIII	GGCC	9 cut(s)	333, 479, 808, 1342, 2058, 2154, 2250, 3072, 3144
HapII	CCGG	7 cut(s)	374, 1244, 1329, 1343, 1455, 3065, 3266
HgaI	GACGC	2 cut(s)	328, 3762
HhaI	GCGC	5 cut(s)	296, 1269, 2151, 2752, 4098
Hin1I	GRCGYC	3 cut(s)	294, 339, 3773
Hin6I	GCGC	5 cut(s)	294, 1267, 2149, 2750, 4096
HinP1I	GCGC	5 cut(s)	294, 1267, 2149, 2750, 4096
HincII	GTYRAC	5 cut(s)	529, 1276, 1771, 2207, 2350
HindII	GTYRAC	5 cut(s)	529, 1276, 1771, 2207, 2350
HindIII	AAGCTT	3 cut(s)	2428, 2901, 4013
HpaII	CCGG	7 cut(s)	374, 1244, 1329, 1343, 1455, 3065, 3266
HphI	GGTGA	6 cut(s)	62, 484, 566, 1469, 2909, 4078
Hpy99I	CGWCG	7 cut(s)	17, 218, 311, 332, 344, 1578, 3778
HpyCH4III	ACNGT	9 cut(s)	45, 218, 491, 1307, 1357, 1812, 1921, 2197, 3533
HpyCH4IV	ACGT	9 cut(s)	49, 257, 398, 525, 977, 1559, 2740, 3361, 3928
HpySE526I	ACGT	9 cut(s)	49, 257, 398, 525, 977, 1559, 2740, 3361, 3928
Hsp92I	GRCGYC	3 cut(s)	294, 339, 3773
HspAI	GCGC	5 cut(s)	294, 1267, 2149, 2750, 4096
KasI	GGCGCC	1 cut(s)	293
KpnI	GGTACC	1 cut(s)	1377
KroI	GCCGGC	2 cut(s)	1328, 1342
KroNI	GCCGGC	2 cut(s)	1330, 1344
Ksp22I	TGATCA	2 cut(s)	1221, 3694
KspI	CCGCGG	1 cut(s)	337
LguI	GCTCTTC	1 cut(s)	2920
MabI	ACCWGGT	1 cut(s)	1366
MaeI	CTAG	7 cut(s)	422, 1482, 1890, 2570, 2621, 3614, 3728
MaeII	ACGT	9 cut(s)	49, 257, 398, 525, 977, 1559, 2740, 3361, 3928
MaeIII	GTNAC	8 cut(s)	45, 394, 1021, 2401, 2717, 3781, 3922, 4066
MbiI	CCGCTC	1 cut(s)	716
MflI	RGATCY	3 cut(s)	1507, 3185, 3250
Mly113I	GGCGCC	1 cut(s)	294
MlyI	GAGTC	8 cut(s)	260, 865, 1174, 1681, 2345, 2855, 3682, 3812
MmeI	TCCRAC	3 cut(s)	1594, 2732, 3756
Mph1103I	ATGCAT	1 cut(s)	4092
MroNI	GCCGGC	2 cut(s)	1328, 1342
MroXI	GAANNNNTTC	3 cut(s)	1971, 3328, 3375
MslI	CAYNNNNRTG	4 cut(s)	687, 1212, 1947, 2112
MspA1I	CMGCKG	3 cut(s)	336, 1496, 2998
MspCI	CTTAAG	2 cut(s)	1879, 3464
MspI	CCGG	7 cut(s)	374, 1244, 1329, 1343, 1455, 3065, 3266
MvaI	CCWGG	7 cut(s)	501, 1110, 1368, 1533, 2247, 3117, 3828
MvnI	CGCG	2 cut(s)	336, 2151
NaeI	GCCGGC	2 cut(s)	1330, 1344
NarI	GGCGCC	1 cut(s)	294
NciI	CCSGG	3 cut(s)	374, 1455, 3266
NcoI	CCATGG	1 cut(s)	1039
NdeI	CATATG	3 cut(s)	1405, 3226, 4072
NgoMIV	GCCGGC	2 cut(s)	1328, 1342
NheI	GCTAGC	1 cut(s)	3613
NmeAIII	GCCGAG	1 cut(s)	1511
NmuCI	GTSAC	3 cut(s)	45, 2717, 4066
NsiI	ATGCAT	1 cut(s)	4092
NspI	RCATGY	4 cut(s)	566, 1052, 2974, 4094
PaeR7I	CTCGAG	1 cut(s)	1182
PagI	TCATGA	4 cut(s)	688, 1584, 1942, 3553
PaqCI	CACCTGC	1 cut(s)	2650
PciI	ACATGT	1 cut(s)	1048
PciSI	GCTCTTC	1 cut(s)	2920
PcsI	WCGNNNNNNNCGW	3 cut(s)	275, 336, 4001
PdiI	GCCGGC	2 cut(s)	1330, 1344
PdmI	GAANNNNTTC	3 cut(s)	1971, 3328, 3375
PfeI	GAWTC	4 cut(s)	303, 359, 2166, 3912
PflMI	CCANNNNNTGG	2 cut(s)	1262, 3833
Ple19I	CGATCG	2 cut(s)	301, 2824
PleI	GAGTC	8 cut(s)	259, 865, 1174, 1680, 2345, 2854, 3682, 3812
PluTI	GGCGCC	1 cut(s)	297
PmaCI	CACGTG	1 cut(s)	3929
PmlI	CACGTG	1 cut(s)	3929
PpsI	GAGTC	8 cut(s)	259, 865, 1174, 1680, 2345, 2854, 3682, 3812
Ppu21I	YACGTR	2 cut(s)	1560, 3929
PpuMI	RGGWCCY	1 cut(s)	748
PscI	ACATGT	1 cut(s)	1048
PshBI	ATTAAT	1 cut(s)	2550
PsiI	TTATAA	2 cut(s)	573, 3096
Psp124BI	GAGCTC	1 cut(s)	2029
Psp1406I	AACGTT	1 cut(s)	3361
Psp5II	RGGWCCY	1 cut(s)	748
Psp6I	CCWGG	7 cut(s)	499, 1108, 1366, 1531, 2245, 3115, 3826
PspCI	CACGTG	1 cut(s)	3929
PspGI	CCWGG	7 cut(s)	499, 1108, 1366, 1531, 2245, 3115, 3826
PspOMI	GGGCCC	1 cut(s)	3142
PspPPI	RGGWCCY	1 cut(s)	748
PspXI	VCTCGAGB	1 cut(s)	1182
PsrI	GAACNNNNNNTAC	2 cut(s)	1233, 1265
PstNI	CAGNNNCTG	2 cut(s)	1172, 3833
PsuI	RGATCY	3 cut(s)	1507, 3185, 3250
PvuI	CGATCG	2 cut(s)	301, 2824
PvuII	CAGCTG	2 cut(s)	1496, 2998
RsaI	GTAC	5 cut(s)	976, 1375, 1809, 2277, 3716
RsaNI	GTAC	5 cut(s)	975, 1374, 1808, 2276, 3715
RseI	CAYNNNNRTG	4 cut(s)	687, 1212, 1947, 2112
SacI	GAGCTC	1 cut(s)	2029
SacII	CCGCGG	1 cut(s)	337
SalI	GTCGAC	1 cut(s)	2205
SapI	GCTCTTC	1 cut(s)	2920
SchI	GAGTC	8 cut(s)	260, 865, 1174, 1681, 2345, 2855, 3682, 3812
SexAI	ACCWGGT	1 cut(s)	1366
SfcI	CTRYAG	1 cut(s)	1716
SfoI	GGCGCC	1 cut(s)	295
Sfr274I	CTCGAG	1 cut(s)	1182
Sfr303I	CCGCGG	1 cut(s)	337
SgrBI	CCGCGG	1 cut(s)	337
SinI	GGWCC	5 cut(s)	370, 748, 1490, 1563, 3824
SlaI	CTCGAG	1 cut(s)	1182
SmiMI	CAYNNNNRTG	4 cut(s)	687, 1212, 1947, 2112
SmlI	CTYRAG	6 cut(s)	875, 1182, 1879, 2802, 3464, 3962
SmoI	CTYRAG	6 cut(s)	875, 1182, 1879, 2802, 3464, 3962
SpeI	ACTAGT	1 cut(s)	2569
SspDI	GGCGCC	1 cut(s)	293
SspMI	CTAG	7 cut(s)	422, 1482, 1890, 2570, 2621, 3614, 3728
SstI	GAGCTC	1 cut(s)	2029
StyI	CCWWGG	2 cut(s)	1039, 2144
TaaI	ACNGT	9 cut(s)	45, 218, 491, 1307, 1357, 1812, 1921, 2197, 3533
TaiI	ACGT	9 cut(s)	52, 260, 401, 528, 980, 1562, 2743, 3364, 3931
TaqII	GACCGA	1 cut(s)	149
TauI	GCSGC	6 cut(s)	204, 207, 336, 339, 2154, 2690
TfiI	GAWTC	4 cut(s)	303, 359, 2166, 3912
TscAI	CASTG	7 cut(s)	445, 1360, 1926, 2011, 2200, 3538, 3738
TseFI	GTSAC	3 cut(s)	45, 2717, 4066
Tsp45I	GTSAC	3 cut(s)	45, 2717, 4066
TspGWI	ACGGA	4 cut(s)	883, 1591, 3924, 4010
TspRI	CASTG	7 cut(s)	445, 1360, 1926, 2011, 2200, 3538, 3738
Van91I	CCANNNNNTGG	2 cut(s)	1262, 3833
Vha464I	CTTAAG	2 cut(s)	1879, 3464
VpaK11BI	GGWCC	5 cut(s)	370, 748, 1490, 1563, 3824
VspI	ATTAAT	1 cut(s)	2550
XapI	RAATTY	6 cut(s)	417, 692, 1937, 2667, 2840, 3620
XbaI	TCTAGA	1 cut(s)	421
XceI	RCATGY	4 cut(s)	566, 1052, 2974, 4094
XhoI	CTCGAG	1 cut(s)	1182
XmiI	GTMKAC	1 cut(s)	2206
XmnI	GAANNNNTTC	3 cut(s)	1971, 3328, 3375
XspI	CTAG	7 cut(s)	422, 1482, 1890, 2570, 2621, 3614, 3728
Zsp2I	ATGCAT	1 cut(s)	4092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.