Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Reverse (-)

29902666 .. 29920044

Gene Structure 17379 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1203100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3936 bp

ATGCCTTGCTCTGCAATCGGCGGTGCTGGCGGAGGCGGTGGGGACAGTAATGGTGCTTTGAGCAATTTCAAGCTCAACGAGTCCACGTTCTTGGCCTCCCTCATGCCGAAGAAAGAGATTGGCGCCGATCGATTCATCGATGCGCATCCCAACTACGACGGCCGCGGCGCCCTGATCGCTATCTTCGATACTGGAGTGGATCCAGCTGCTTCTGGATTACAAGTTACGTCAGACGGAAAGCCGAAAATCCTAGATGTTCTTGATTGCACCGGGAGTGGTGATGTTGATACTTCGAGGGTAGTCAAGGCCGATGACGATGGTTGTATTTGTGGAGCTTCGGGAGCATCTCTGTTTGTCAACTCTTCATGGAAAAATCCTTCCGGTGAATGGCGTGTCGGCTATAAATTGCTATATGAGCTATTTACGGATACGTTGACTTCTCGCTTGAAGAGAGAAAGAAGGAAAAAATGGGATAAACAAAACCAAGAAGAAATTGCCAAGGCTGTTAAACATCTTCAAGAATTTGATCAGAAACACAGCAAATTGGATGATGTTAACTTGAAAAGAGCTCGGGAGGACCTGCAAAATAGAGTTGATTACCTACAAAAGCAAGCTGATAGCTATGATGACAAAGGGCCTGTCATAGATGCTGTTGTATGGCATGATGGAGAAGTATGGAGGGTTGCCCTTGACACACAGACTCTTGAGGATAACCCAGATTGTGGAAAACTTGCAAACTTTGTGCCCCTAACTAATTATAGGATAGAACGAAAATATGGTGTTTTTAGCAAATTAGACGCCTGTACATTTGTTGCTAATGTGTACGATGAAGGGAATATTCTAAGTATCGTAACAGATTGCCATCCACATGGCACTCATGTTGCTGGTATCGCTACTGCTTTCCACCCGAAGGAACCCCTGTTGAATGGAGTTGCACCTGGAGCACAAGTCATATCTTGTAAGATTGGAGACTCACGTTTAGGTTCAATAGAGACTGGAACTGGGTTGACTCGAGCCTTGATTGCTGCTGTGGAGCATAAATGTGATTTGATCAACATGAGTTATGGAGAACCTACACTGCTGCCAGACTATGGGCGTTTTGTTGACCTCGTTAATGAAGCTGTGAACAAGCACCGTCTGATATTTGTGAGCAGTGCTGGCAATAATGGGCCAGCATTGAGCACTGTTGGAGCACCTGGTGGTACCACATCAAGCATTATAGGAGTTGGTGCATATGTCTCTCCTGCAATGGCTGCTGGTGCTCATTGTGTAGTTGAAGCTCCAGGCGAAGGGCTTGAGTACACTTGGTCTAGCCGCGGGCCAACCAGTGATGGAGCTCTTGGTGTCTGTATAAGTGCTCCTGGTGCAGCTGTTGCTCCTGTTCCTACATGGACTCTTCAGCGACGCATGCTCATGAATGGAACATCAATGTCATCTCCATCTGCTTGTGGGGGAATTGCATTGCTTGTTAGTGCAATGAAGGCTGAGGGTATTCCTGTGAGTCCATACAGTGTAAGGAAGGCTCTTGAGAATACATCTGTTCCTATAGGCTGTTTGCCTGAAGATAAACTATCAACTGGACAAGGGCTTATGCAAGTTGACAAGGCACATGAATATCTAAGGCAGAGCCGGGATGTACCAAGTGTTTGGTATCAGATAAAGATAAATCAATCTGGTAAAACAACTTCTACAACACGAGGAATCTACTTAAGGGAGGCTAGTGCTTTCCAACAATCTACCGAGTGGACGGTGCAAGTTGAACCAAAATTTCATGAAGGTGCTAGTAATTTGGAAGATTTGGTTCCTTTTGAAGAGTGTATTGAGCTACATTCCAGTGAGAAGGCTATTTTGAGGGCTCCTGATTTTCTCCTTCTTACCCATAATGGGCGTAGCTTCAACATAGTAGTGGATCCCACCAACCTTAGTGAAGGTCTGCACTATTTCGAACTGTATGGTGTTGATTGTAAAGCACCATGGCGCGGTCCTCTTTTCAGAATCCCTGTTACCATAACAAAGCCTATAGCTGTGATAAGTCGACCTCCATTGTTGTCATTCTCTAGGATATCATTTTTGCCAGGCCACATAGAAAGGAGATTTATAGAAGTGCCTCTTGGTGCTACATGGGTTGAAGCAACTATGCAAACATCAGGATTTGATACAGCAAGAAGATTTTTTATTGACTCTGTTCAGCTATGTCCCCTGCAAAGGCCTCTTAAATGGGAGAGTGTTGTAATATTCTCGTCTCCTGCTGCCAAAAGCTTTTCATTTCCTGTTGTGGGTGGTCAAACAATGGAATTAGCTATTGCTCAATTTTGGTCAAGTGGCATAGGAAGCCATGAAACAACTATTGTGGATTTTGAGATTGTGTTTCATGGAATTAGCATTAATAAAGATGAAGTAGTGCTTGATGGAAGTGAAGGGCCAACCAGAATTGAAGCTGAAGCTCTACTGGCTTCTGAGACACTTGCACCGGTTGCTATTCTAAACAAGATAAGAATTCCATACCGGCCGGTTGAGTCTAAACTTTGTTCACTCTCAACAGATCGTGACAAACTACCTTCAGAAAAACGTATTATGGCACTTACTTTAACCTACAAGATCAAATTGGAAGACGGGGCTGAAGTGAAGCCTCAAGTTCCATTACTTAACAATCGCGTATATGACACCAAATTGGAGTCTCAATTTTATATGATCTCTGATGCAAACAAGCGTATATATGCAATGGGTGACATATATCCAAGTAAATCAAGACTGCCCAAGGGAGATTACAATTTACGGCTTTATCTAAGACATGACAATGTGCAGTACTTGGAAAAACTGAAGCAGCTGGTGTTGTTCATCGAGAGAAATTTGGAAGAGAAGGATGTAATCCGGTTGAGCTTCTTCTCCCAACCTGATGGCCCTTTGATGGGAAATGGTAGTTTTAAGTCCTCAGTATTAGTCCCAGGGAAAAAAGAAGCATTCTATTTGGGTCCACCATCCAAAGACAAGCTTCTAAAGTTTTCTTCACAAGGATCTGTATTACTTGGAGCTATCTCGTACGGGAAGCTGTCATATGTTGATAAGGGAGAGGGAAAAAATCCACTAAAGAACCCTGTGTCTTATCAGATTTCTTATATAGTGCCACCCAATAAGATGGATGAGGACAAAGAAAAAGGTTCTTCTACTTGCACCAAGCCTGTTTCTGAGCGGTTAAAGGAAGAGGTTCGAGATGCAAAGATTAAAGTTCTTGCAAGCCTGAAGCAAGATACTGAAGAAGATTGTTTGGAGTGGAAAAAGTTATCGTCTTCTCTTAAGTCTGAATATCCTAAATACACCCCACTGTTTGCAAAGATCTTGGAAGCTGTACTTTCTCGGAGCAATGACAAGGACAAAGTCTGCCATGAAAAAGAGGTAATTGATGCAGCGAATGAGGTGGTTGACAGTGTTGATAGAGACGAGCTGGCAAAATTTTTTGCACTAAGAAGTGATCCCGATGATGAAGAGGCTGAGAAAATTAAAAAGAAGATGGAGACAACTCGTGATCAATTAGCAGAGGCATTGTACCAGAAAGGACTGGCACTGGCAGAGATTGAATCTTTGCAGGGTGAGAAGCCTGCAAAGGCTGAAGGTGCAGAAGGGGGGGAGAAGATTGAAGATCCTTTGAAACCAGGCTCAGATTTGTTTGAAAGCAACTTTAAAGAACTTCAAAAATGGGTTGAAGTGAAATCCTCCAAATACGGAACTCTCTCAGTACTACGTGAGAAACGTTCTGGAAGGCTTGGAACAGCACTGAAGGTTCTGAATGATGTAATTCAAGACGATGGGGAGCCTCCCAAGAAGAAGTTATACGACCTGACGATTTCCTTGCTCGATGAGATTGGCTGGCAACATTTGGCGGCACACGAGAGGCAATGGATGCATGTCCGCTTTCCACCAAGTTTACCTCTTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1312

Amino Acids

144.33

Weight (kDa)

5.91

Isoelectric Point (pI)

40.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	54 - 513	2.4e-44	Subtilase family
TPPII_Ig-like-1	PF21223	562 - 670	8.3e-23	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	690 - 774	6e-14	TPPII, galactose-binding domain-like
TPPII	PF12580	816 - 1000	9.2e-60	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	144
Acc36I	ACCTGC	1 cut(s)	588
Acc65I	GGTACC	1 cut(s)	1202
AccB1I	GGYRCC	3 cut(s)	122, 167, 1202
AccB7I	CCANNNNNTGG	2 cut(s)	722, 1091
AccBSI	CCGCTC	1 cut(s)	3190
AccI	GTMKAC	1 cut(s)	2035
AccII	CGCG	4 cut(s)	165, 1317, 1980, 2655
AclI	AACGTT	1 cut(s)	3748
AclWI	GGATC	7 cut(s)	194, 207, 1904, 1917, 3022, 3464, 3632
AcoI	YGGCCR	2 cut(s)	160, 2507
AcsI	RAATTY	5 cut(s)	521, 1766, 2496, 2848, 3449
AcyI	GRCGYC	3 cut(s)	123, 168, 798
AdeI	CACNNNGTG	1 cut(s)	1199
AflII	CTTAAG	2 cut(s)	1708, 3293
AgeI	ACCGGT	1 cut(s)	2470
AjnI	CCWGG	7 cut(s)	937, 1195, 1282, 1360, 2074, 2944, 3649
AjuI	GAANNNNNNNTTGG	2 cut(s)	2094, 2126
Alw21I	GWGCWC	7 cut(s)	571, 946, 1184, 1195, 1264, 1339, 1360
Alw26I	GTCTC	8 cut(s)	963, 986, 1243, 2247, 2453, 2682, 3429, 3506
AlwI	GGATC	7 cut(s)	194, 207, 1904, 1917, 3022, 3464, 3632
Ama87I	CYCGRG	2 cut(s)	570, 1011
ApeKI	GCWGC	8 cut(s)	206, 1025, 1081, 1253, 1367, 2249, 2824, 3404
ApoI	RAATTY	5 cut(s)	521, 1766, 2496, 2848, 3449
ArsI	GACNNNNNNTTYG	2 cut(s)	624, 656
AseI	ATTAAT	1 cut(s)	2385
AsiGI	ACCGGT	1 cut(s)	2470
Asp700I	GAANNNNTTC	1 cut(s)	3204
Asp718I	GGTACC	1 cut(s)	1202
AspLEI	GCGC	4 cut(s)	125, 145, 170, 1980
AspS9I	GGNCC	8 cut(s)	577, 635, 1169, 1319, 1982, 2420, 2900, 2972
AsuC2I	CCSGG	2 cut(s)	271, 1631
AsuHPI	GGTGA	4 cut(s)	290, 395, 2738, 3599
AsuII	TTCGAA	1 cut(s)	1944
AvaI	CYCGRG	2 cut(s)	570, 1011
AvaII	GGWCC	3 cut(s)	577, 1982, 2972
BaeGI	GKGCMC	1 cut(s)	747
BaeI	ACNNNNGTAYC	2 cut(s)	180, 213
BamHI	GGATCC	2 cut(s)	199, 1909
BanI	GGYRCC	3 cut(s)	122, 167, 1202
BanII	GRGCYC	3 cut(s)	571, 1339, 1858
BarI	GAAGNNNNNNTAC	4 cut(s)	1669, 1701, 3812, 3844
BauI	CACGAG	3 cut(s)	1695, 3519, 3884
BbsI	GAAGAC	2 cut(s)	2616, 3279
Bbv12I	GWGCWC	7 cut(s)	571, 946, 1184, 1195, 1264, 1339, 1360
BbvCI	CCTCAGC	1 cut(s)	1485
BbvI	GCAGC	8 cut(s)	193, 1012, 1068, 1240, 1379, 2236, 2836, 3416
BceAI	ACGGC	2 cut(s)	175, 2792
BcgI	CGANNNNNNTGC	2 cut(s)	3829, 3863
BciT130I	CCWGG	7 cut(s)	939, 1197, 1284, 1362, 2076, 2946, 3651
BciVI	GTATCC	1 cut(s)	421
BclI	TGATCA	3 cut(s)	526, 1050, 3523
BcnI	CCSGG	2 cut(s)	271, 1631
BcoDI	GTCTC	8 cut(s)	963, 986, 1243, 2247, 2453, 2682, 3429, 3506
BfaI	CTAG	5 cut(s)	251, 1311, 1719, 1782, 2058
BfmI	CTRYAG	2 cut(s)	1545, 2019
BfoI	RGCGCY	2 cut(s)	126, 171
BfrI	CTTAAG	2 cut(s)	1708, 3293
BfuAI	ACCTGC	1 cut(s)	588
BfuI	GTATCC	1 cut(s)	421
BglII	AGATCT	1 cut(s)	3333
BmcAI	AGTACT	2 cut(s)	2807, 3735
Bme1390I	CCNGG	9 cut(s)	271, 939, 1197, 1284, 1362, 1631, 2076, 2946, 3651
Bme18I	GGWCC	3 cut(s)	577, 1982, 2972
BmeT110I	CYCGRG	2 cut(s)	570, 1011
BmgT120I	GGNCC	8 cut(s)	577, 635, 1169, 1319, 1982, 2420, 2900, 2972
BmrFI	CCNGG	9 cut(s)	271, 939, 1197, 1284, 1362, 1631, 2076, 2946, 3651
BmrI	ACTGGG	1 cut(s)	1011
BmsI	GCATC	8 cut(s)	130, 154, 353, 637, 2689, 3202, 3391, 3888
BmuI	ACTGGG	1 cut(s)	1011
BpiI	GAAGAC	2 cut(s)	2616, 3279
BpmI	CTGGAG	3 cut(s)	213, 960, 1266
Bpu10I	CCTNAGC	1 cut(s)	1485
Bpu14I	TTCGAA	1 cut(s)	1944
BpuEI	CTTGAG	4 cut(s)	725, 1316, 1547, 2616
BpuMI	CCSGG	2 cut(s)	271, 1631
Bsa29I	ATCGAT	2 cut(s)	130, 138
BsaAI	YACGTR	1 cut(s)	3740
BsaBI	GATNNNNATC	3 cut(s)	144, 179, 861
BsaHI	GRCGYC	3 cut(s)	123, 168, 798
BsaJI	CCNNGG	7 cut(s)	163, 498, 1315, 1973, 2757, 2944, 2945
BsaWI	WCCGGW	3 cut(s)	380, 2470, 2871
BsaXI	ACNNNNNCTCC	2 cut(s)	933, 963
Bse118I	RCCGGY	3 cut(s)	2470, 2505, 2509
Bse1I	ACTGG	9 cut(s)	196, 1000, 1006, 1326, 1582, 1833, 2454, 3561, 3567
Bse3DI	GCAATG	6 cut(s)	1254, 1460, 1482, 2727, 3367, 3899
Bse8I	GATNNNNATC	3 cut(s)	144, 179, 861
BseBI	CCWGG	7 cut(s)	939, 1197, 1284, 1362, 2076, 2946, 3651
BseCI	ATCGAT	2 cut(s)	130, 138
BseDI	CCNNGG	7 cut(s)	163, 498, 1315, 1973, 2757, 2944, 2945
BseGI	GGATG	8 cut(s)	145, 553, 862, 1639, 2869, 2978, 3145, 3903
BseJI	GATNNNNATC	3 cut(s)	144, 179, 861
BseMI	GCAATG	6 cut(s)	1254, 1460, 1482, 2727, 3367, 3899
BseMII	CTCAG	7 cut(s)	1476, 2448, 2946, 3177, 3480, 3669, 3744
BseNI	ACTGG	9 cut(s)	196, 1000, 1006, 1326, 1582, 1833, 2454, 3561, 3567
BseSI	GKGCMC	1 cut(s)	747
BseX3I	CGGCCG	2 cut(s)	160, 2507
BseXI	GCAGC	8 cut(s)	193, 1012, 1068, 1240, 1379, 2236, 2836, 3416
BsgI	GTGCAG	4 cut(s)	1386, 1919, 2822, 3633
Bsh1236I	CGCG	4 cut(s)	165, 1317, 1980, 2655
Bsh1285I	CGRYCG	3 cut(s)	130, 163, 2510
BshNI	GGYRCC	3 cut(s)	122, 167, 1202
BshTI	ACCGGT	1 cut(s)	2470
BshVI	ATCGAT	2 cut(s)	130, 138
BsiEI	CGRYCG	3 cut(s)	130, 163, 2510
BsiHKAI	GWGCWC	7 cut(s)	571, 946, 1184, 1195, 1264, 1339, 1360
BsiHKCI	CYCGRG	2 cut(s)	570, 1011
BsiSI	CCGG	7 cut(s)	270, 381, 1630, 2471, 2506, 2510, 2872
BsiWI	CGTACG	1 cut(s)	3039
BslFI	GGGAC	3 cut(s)	56, 2181, 2927
BsmAI	GTCTC	8 cut(s)	963, 986, 1243, 2247, 2453, 2682, 3429, 3506
BsmBI	CGTCTC	2 cut(s)	2247, 3429
BsmFI	GGGAC	3 cut(s)	56, 2181, 2927
BsmI	GAATGC	1 cut(s)	2960
BsoBI	CYCGRG	2 cut(s)	570, 1011
Bsp119I	TTCGAA	1 cut(s)	1944
Bsp1286I	GDGCHC	9 cut(s)	571, 747, 946, 1184, 1195, 1264, 1339, 1360, 1858
Bsp1407I	TGTACA	1 cut(s)	803
Bsp19I	CCATGG	1 cut(s)	1973
BspCNI	CTCAG	7 cut(s)	1477, 2449, 2945, 3178, 3481, 3668, 3743
BspDI	ATCGAT	2 cut(s)	130, 138
BspFNI	CGCG	4 cut(s)	165, 1317, 1980, 2655
BspHI	TCATGA	2 cut(s)	1413, 1771
BspMI	ACCTGC	1 cut(s)	588
BspPI	GGATC	7 cut(s)	194, 207, 1904, 1917, 3022, 3464, 3632
BspT104I	TTCGAA	1 cut(s)	1944
BspT107I	GGYRCC	3 cut(s)	122, 167, 1202
BspTI	CTTAAG	2 cut(s)	1708, 3293
BsrBI	CCGCTC	1 cut(s)	3190
BsrDI	GCAATG	6 cut(s)	1254, 1460, 1482, 2727, 3367, 3899
BsrFI	RCCGGY	3 cut(s)	2470, 2505, 2509
BsrGI	TGTACA	1 cut(s)	803
BsrI	ACTGG	9 cut(s)	196, 1000, 1006, 1326, 1582, 1833, 2454, 3561, 3567
BssAI	RCCGGY	3 cut(s)	2470, 2505, 2509
BssECI	CCNNGG	7 cut(s)	163, 498, 1315, 1973, 2757, 2944, 2945
BssNI	GRCGYC	3 cut(s)	123, 168, 798
BssSI	CACGAG	3 cut(s)	1695, 3519, 3884
BssT1I	CCWWGG	3 cut(s)	498, 1973, 2757
Bst2BI	CACGAG	3 cut(s)	1695, 3519, 3884
Bst2UI	CCWGG	7 cut(s)	939, 1197, 1284, 1362, 2076, 2946, 3651
Bst4CI	ACNGT	8 cut(s)	47, 1136, 1186, 1511, 1750, 1950, 3324, 3425
Bst6I	CTCTTC	7 cut(s)	367, 443, 1401, 1806, 2850, 3195, 3477
BstACI	GRCGYC	3 cut(s)	123, 168, 798
BstAFI	CTTAAG	2 cut(s)	1708, 3293
BstAPI	GCANNNNNTGC	4 cut(s)	1253, 1373, 2474, 3898
BstAUI	TGTACA	1 cut(s)	803
BstBAI	YACGTR	1 cut(s)	3740
BstBI	TTCGAA	1 cut(s)	1944
BstDSI	CCRYGG	3 cut(s)	163, 1315, 1973
BstF5I	GGATG	8 cut(s)	145, 553, 862, 1639, 2869, 2978, 3145, 3903
BstFNI	CGCG	4 cut(s)	165, 1317, 1980, 2655
BstH2I	RGCGCY	2 cut(s)	126, 171
BstHHI	GCGC	4 cut(s)	125, 145, 170, 1980
BstMAI	GTCTC	8 cut(s)	963, 986, 1243, 2247, 2453, 2682, 3429, 3506
BstMCI	CGRYCG	3 cut(s)	130, 163, 2510
BstNI	CCWGG	7 cut(s)	939, 1197, 1284, 1362, 2076, 2946, 3651
BstNSI	RCATGY	2 cut(s)	1411, 3905
BstSCI	CCNGG	9 cut(s)	269, 937, 1195, 1282, 1360, 1629, 2074, 2944, 3649
BstSFI	CTRYAG	2 cut(s)	1545, 2019
BstSLI	GKGCMC	1 cut(s)	747
BstUI	CGCG	4 cut(s)	165, 1317, 1980, 2655
BstV1I	GCAGC	8 cut(s)	193, 1012, 1068, 1240, 1379, 2236, 2836, 3416
BstV2I	GAAGAC	2 cut(s)	2616, 3279
BstX2I	RGATCY	5 cut(s)	199, 1909, 3014, 3333, 3637
BstXI	CCANNNNNNTGG	5 cut(s)	91, 869, 1647, 2897, 3136
BstYI	RGATCY	5 cut(s)	199, 1909, 3014, 3333, 3637
BstZI	CGGCCG	2 cut(s)	160, 2507
Bsu15I	ATCGAT	2 cut(s)	130, 138
BsuI	GTATCC	1 cut(s)	421
BsuTUI	ATCGAT	2 cut(s)	130, 138
BtgI	CCRYGG	3 cut(s)	163, 1315, 1973
BtsCI	GGATG	8 cut(s)	145, 553, 862, 1639, 2869, 2978, 3145, 3903
BtsI	GCAGTG	2 cut(s)	1076, 1159
BveI	ACCTGC	1 cut(s)	588
CciI	TCATGA	2 cut(s)	1413, 1771
CfoI	GCGC	4 cut(s)	125, 145, 170, 1980
Cfr10I	RCCGGY	3 cut(s)	2470, 2505, 2509
Cfr13I	GGNCC	8 cut(s)	577, 635, 1169, 1319, 1982, 2420, 2900, 2972
Cfr42I	CCGCGG	2 cut(s)	166, 1318
ClaI	ATCGAT	2 cut(s)	130, 138
CseI	GACGC	2 cut(s)	806, 1413
CsiI	ACCWGGT	1 cut(s)	1195
CspAI	ACCGGT	1 cut(s)	2470
CspCI	CAANNNNNGTGG	2 cut(s)	2331, 2366
DinI	GGCGCC	2 cut(s)	124, 169
DraI	TTTAAA	1 cut(s)	3679
DraIII	CACNNNGTG	1 cut(s)	1199
EaeI	YGGCCR	2 cut(s)	160, 2507
EagI	CGGCCG	2 cut(s)	160, 2507
Eam1104I	CTCTTC	7 cut(s)	367, 443, 1401, 1806, 2850, 3195, 3477
EarI	CTCTTC	7 cut(s)	367, 443, 1401, 1806, 2850, 3195, 3477
EciI	GGCGGA	1 cut(s)	45
Ecl136II	GAGCTC	2 cut(s)	569, 1337
EclXI	CGGCCG	2 cut(s)	160, 2507
Eco130I	CCWWGG	3 cut(s)	498, 1973, 2757
Eco147I	AGGCCT	1 cut(s)	2209
Eco24I	GRGCYC	3 cut(s)	571, 1339, 1858
Eco32I	GATATC	1 cut(s)	2064
Eco47I	GGWCC	3 cut(s)	577, 1982, 2972
Eco52I	CGGCCG	2 cut(s)	160, 2507
Eco53kI	GAGCTC	2 cut(s)	569, 1337
Eco88I	CYCGRG	2 cut(s)	570, 1011
EcoICRI	GAGCTC	2 cut(s)	569, 1337
EcoO109I	RGGNCCY	2 cut(s)	577, 635
EcoRI	GAATTC	1 cut(s)	2496
EcoRII	CCWGG	7 cut(s)	937, 1195, 1282, 1360, 2074, 2944, 3649
EcoRV	GATATC	1 cut(s)	2064
EcoT14I	CCWWGG	3 cut(s)	498, 1973, 2757
EcoT22I	ATGCAT	1 cut(s)	3903
EcoT38I	GRGCYC	3 cut(s)	571, 1339, 1858
EgeI	GGCGCC	2 cut(s)	124, 169
EheI	GGCGCC	2 cut(s)	124, 169
ErhI	CCWWGG	3 cut(s)	498, 1973, 2757
Esp3I	CGTCTC	2 cut(s)	2247, 3429
FalI	AAGNNNNNCTT	8 cut(s)	2094, 2126, 2388, 2420, 2976, 3008, 3333, 3365
FaqI	GGGAC	3 cut(s)	56, 2181, 2927
FauI	CCCGC	1 cut(s)	1310
FauNDI	CATATG	2 cut(s)	1234, 3055
FbaI	TGATCA	3 cut(s)	526, 1050, 3523
FblI	GTMKAC	1 cut(s)	2035
FokI	GGATG	8 cut(s)	132, 560, 849, 1646, 2876, 2965, 3152, 3910
FriOI	GRGCYC	3 cut(s)	571, 1339, 1858
FspAI	RTGCGCAY	1 cut(s)	144
FspBI	CTAG	5 cut(s)	251, 1311, 1719, 1782, 2058
FspI	TGCGCA	1 cut(s)	144
GlaI	GCGC	4 cut(s)	124, 144, 169, 1979
GsuI	CTGGAG	3 cut(s)	213, 960, 1266
HaeII	RGCGCY	2 cut(s)	126, 171
HapII	CCGG	7 cut(s)	270, 381, 1630, 2471, 2506, 2510, 2872
HgaI	GACGC	2 cut(s)	806, 1413
HhaI	GCGC	4 cut(s)	125, 145, 170, 1980
Hin1I	GRCGYC	3 cut(s)	123, 168, 798
Hin6I	GCGC	4 cut(s)	123, 143, 168, 1978
HinP1I	GCGC	4 cut(s)	123, 143, 168, 1978
HincII	GTYRAC	8 cut(s)	358, 435, 556, 1008, 1105, 1600, 2036, 3421
HindII	GTYRAC	8 cut(s)	358, 435, 556, 1008, 1105, 1600, 2036, 3421
HindIII	AAGCTT	2 cut(s)	2257, 2990
HpaI	GTTAAC	1 cut(s)	556
HpaII	CCGG	7 cut(s)	270, 381, 1630, 2471, 2506, 2510, 2872
HphI	GGTGA	4 cut(s)	290, 395, 2738, 3599
Hpy99I	CGWCG	2 cut(s)	161, 1407
HpyCH4III	ACNGT	8 cut(s)	47, 1136, 1186, 1511, 1750, 1950, 3324, 3425
HpyCH4IV	ACGT	7 cut(s)	86, 227, 431, 976, 2569, 3739, 3748
HpySE526I	ACGT	7 cut(s)	86, 227, 431, 976, 2569, 3739, 3748
Hsp92I	GRCGYC	3 cut(s)	123, 168, 798
HspAI	GCGC	4 cut(s)	123, 143, 168, 1978
KasI	GGCGCC	2 cut(s)	122, 167
KpnI	GGTACC	1 cut(s)	1206
Ksp22I	TGATCA	3 cut(s)	526, 1050, 3523
KspAI	GTTAAC	1 cut(s)	556
KspI	CCGCGG	2 cut(s)	166, 1318
Lsp1109I	GCAGC	8 cut(s)	193, 1012, 1068, 1240, 1379, 2236, 2836, 3416
LweI	GCATC	8 cut(s)	130, 154, 353, 637, 2689, 3202, 3391, 3888
MabI	ACCWGGT	1 cut(s)	1195
MaeI	CTAG	5 cut(s)	251, 1311, 1719, 1782, 2058
MaeII	ACGT	7 cut(s)	86, 227, 431, 976, 2569, 3739, 3748
MaeIII	GTNAC	5 cut(s)	223, 850, 2002, 2546, 2726
MbiI	CCGCTC	1 cut(s)	3190
MflI	RGATCY	5 cut(s)	199, 1909, 3014, 3333, 3637
MhlI	GDGCHC	9 cut(s)	571, 747, 946, 1184, 1195, 1264, 1339, 1360, 1858
Mly113I	GGCGCC	2 cut(s)	123, 168
MlyI	GAGTC	9 cut(s)	89, 694, 965, 1003, 1387, 1510, 2174, 2525, 2684
MmeI	TCCRAC	2 cut(s)	1168, 1753
Mph1103I	ATGCAT	1 cut(s)	3903
MroXI	GAANNNNTTC	1 cut(s)	3204
MslI	CAYNNNNRTG	7 cut(s)	867, 1041, 1412, 1776, 1833, 1904, 2796
MspA1I	CMGCKG	5 cut(s)	165, 206, 1317, 1370, 2827
MspCI	CTTAAG	2 cut(s)	1708, 3293
MspI	CCGG	7 cut(s)	270, 381, 1630, 2471, 2506, 2510, 2872
MspR9I	CCNGG	9 cut(s)	271, 939, 1197, 1284, 1362, 1631, 2076, 2946, 3651
Mva1269I	GAATGC	1 cut(s)	2960
MvaI	CCWGG	7 cut(s)	939, 1197, 1284, 1362, 2076, 2946, 3651
MvnI	CGCG	4 cut(s)	165, 1317, 1980, 2655
NarI	GGCGCC	2 cut(s)	123, 168
NciI	CCSGG	2 cut(s)	271, 1631
NcoI	CCATGG	1 cut(s)	1973
NdeI	CATATG	2 cut(s)	1234, 3055
NmuCI	GTSAC	2 cut(s)	2546, 2726
NsbI	TGCGCA	1 cut(s)	144
NsiI	ATGCAT	1 cut(s)	3903
NspI	RCATGY	2 cut(s)	1411, 3905
NspV	TTCGAA	1 cut(s)	1944
PaeI	GCATGC	1 cut(s)	1411
PaeR7I	CTCGAG	1 cut(s)	1011
PagI	TCATGA	2 cut(s)	1413, 1771
PasI	CCCWGGG	1 cut(s)	2945
PceI	AGGCCT	1 cut(s)	2209
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 3745
PctI	GAATGC	1 cut(s)	2960
PdmI	GAANNNNTTC	1 cut(s)	3204
PfeI	GAWTC	4 cut(s)	132, 1701, 1995, 3575
Pfl23II	CGTACG	1 cut(s)	3039
PflFI	GACNNNGTC	1 cut(s)	3374
PflMI	CCANNNNNTGG	2 cut(s)	722, 1091
PinAI	ACCGGT	1 cut(s)	2470
Ple19I	CGATCG	1 cut(s)	130
PleI	GAGTC	9 cut(s)	88, 694, 965, 1003, 1387, 1509, 2174, 2524, 2683
PluTI	GGCGCC	2 cut(s)	126, 171
PpsI	GAGTC	9 cut(s)	88, 694, 965, 1003, 1387, 1509, 2174, 2524, 2683
Ppu21I	YACGTR	1 cut(s)	3740
PpuMI	RGGWCCY	1 cut(s)	577
PshBI	ATTAAT	1 cut(s)	2385
Psp124BI	GAGCTC	2 cut(s)	571, 1339
Psp1406I	AACGTT	1 cut(s)	3748
Psp5II	RGGWCCY	1 cut(s)	577
Psp6I	CCWGG	7 cut(s)	937, 1195, 1282, 1360, 2074, 2944, 3649
PspGI	CCWGG	7 cut(s)	937, 1195, 1282, 1360, 2074, 2944, 3649
PspLI	CGTACG	1 cut(s)	3039
PspPI	GGNCC	8 cut(s)	577, 635, 1169, 1319, 1982, 2420, 2900, 2972
PspPPI	RGGWCCY	1 cut(s)	577
PspXI	VCTCGAGB	1 cut(s)	1011
PsuI	RGATCY	5 cut(s)	199, 1909, 3014, 3333, 3637
PsyI	GACNNNGTC	1 cut(s)	3374
PvuI	CGATCG	1 cut(s)	130
PvuII	CAGCTG	3 cut(s)	206, 1370, 2827
RseI	CAYNNNNRTG	7 cut(s)	867, 1041, 1412, 1776, 1833, 1904, 2796
SacI	GAGCTC	2 cut(s)	571, 1339
SacII	CCGCGG	2 cut(s)	166, 1318
SalI	GTCGAC	1 cut(s)	2034
Sau96I	GGNCC	8 cut(s)	577, 635, 1169, 1319, 1982, 2420, 2900, 2972
ScaI	AGTACT	2 cut(s)	2807, 3735
SchI	GAGTC	9 cut(s)	89, 694, 965, 1003, 1387, 1510, 2174, 2525, 2684
ScrFI	CCNGG	9 cut(s)	271, 939, 1197, 1284, 1362, 1631, 2076, 2946, 3651
SduI	GDGCHC	9 cut(s)	571, 747, 946, 1184, 1195, 1264, 1339, 1360, 1858
SexAI	ACCWGGT	1 cut(s)	1195
SfaNI	GCATC	8 cut(s)	130, 154, 353, 637, 2689, 3202, 3391, 3888
SfcI	CTRYAG	2 cut(s)	1545, 2019
SfoI	GGCGCC	2 cut(s)	124, 169
Sfr274I	CTCGAG	1 cut(s)	1011
Sfr303I	CCGCGG	2 cut(s)	166, 1318
SfuI	TTCGAA	1 cut(s)	1944
SgrBI	CCGCGG	2 cut(s)	166, 1318
SinI	GGWCC	3 cut(s)	577, 1982, 2972
SlaI	CTCGAG	1 cut(s)	1011
SmiMI	CAYNNNNRTG	7 cut(s)	867, 1041, 1412, 1776, 1833, 1904, 2796
SmlI	CTYRAG	7 cut(s)	704, 1011, 1295, 1526, 1708, 2631, 3293
SmoI	CTYRAG	7 cut(s)	704, 1011, 1295, 1526, 1708, 2631, 3293
SphI	GCATGC	1 cut(s)	1411
SseBI	AGGCCT	1 cut(s)	2209
SspDI	GGCGCC	2 cut(s)	122, 167
SspI	AATATT	2 cut(s)	838, 2235
SspMI	CTAG	5 cut(s)	251, 1311, 1719, 1782, 2058
SstI	GAGCTC	2 cut(s)	571, 1339
StuI	AGGCCT	1 cut(s)	2209
StyD4I	CCNGG	9 cut(s)	269, 937, 1195, 1282, 1360, 1629, 2074, 2944, 3649
StyI	CCWWGG	3 cut(s)	498, 1973, 2757
TaaI	ACNGT	8 cut(s)	47, 1136, 1186, 1511, 1750, 1950, 3324, 3425
TaiI	ACGT	7 cut(s)	89, 230, 434, 979, 2572, 3742, 3751
TatI	WGTACW	5 cut(s)	803, 1299, 2805, 3346, 3733
TauI	GCSGC	4 cut(s)	165, 168, 1317, 3881
TfiI	GAWTC	4 cut(s)	132, 1701, 1995, 3575
TseFI	GTSAC	2 cut(s)	2546, 2726
TseI	GCWGC	8 cut(s)	206, 1025, 1081, 1253, 1367, 2249, 2824, 3404
Tsp45I	GTSAC	2 cut(s)	2546, 2726
TspGWI	ACGGA	3 cut(s)	249, 440, 3735
Tth111I	GACNNNGTC	1 cut(s)	3374
Van91I	CCANNNNNTGG	2 cut(s)	722, 1091
Vha464I	CTTAAG	2 cut(s)	1708, 3293
VpaK11BI	GGWCC	3 cut(s)	577, 1982, 2972
VspI	ATTAAT	1 cut(s)	2385
XapI	RAATTY	5 cut(s)	521, 1766, 2496, 2848, 3449
XceI	RCATGY	2 cut(s)	1411, 3905
XhoI	CTCGAG	1 cut(s)	1011
XmiI	GTMKAC	1 cut(s)	2035
XmnI	GAANNNNTTC	1 cut(s)	3204
XspI	CTAG	5 cut(s)	251, 1311, 1719, 1782, 2058
ZrmI	AGTACT	2 cut(s)	2807, 3735
Zsp2I	ATGCAT	1 cut(s)	3903

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.