Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Reverse (-)

62896624 .. 62908931

Gene Structure 12308 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG411700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4134 bp

ATGCAAAACGCCGTCGTATTATTCAAATTACCTTATTTGCCATCGTCACGTGACTCACGTCTCCTCCCACTTCATCATCACTATCACTCGCTAATCATTAGACCCAAAAAGCCGAGCAGAGCGAGACGAAGACCAAGAGAGACCGAGTGGAGTGATCGGAGTAGAACCAGTAGCGTTAGGGTTAGGGCAATGCCTTGCTCTGCGATTAGCGGCGGCGGTAGCGACGGTAATGGTTCTCTGCGTAATTTCAAGCTCAACGAGTCCACGTTCTTAGCCTCGCTCATGCCGAAGAAGGAGATCGGCGCCGATCGATTCGTCGAGGCTCATCCGAATTACGACGGCCGTGGCGTCGTTATTGCTATCTTCGATTCTGGAGTGGACCCGGCTGCTGCTGGATTACAGGTTACGTCAGATGGAAAACCGAAAATTCTAGATGTTCTTGACTGCACTGGGAGTGGAGATGTTGATACTTCAAAGGTGGTGAAGGCCGATGAGAACGGTTGCATACCTGGAGCTTCAGGGGCATCTCTGCACGTCAACTCTTCATGGAAAAATCCTTCTGGTGAATGGCACGTGGGTTATAAGTTGGTTTACGAGCTATTTACTAACACATTGACTTCCCGCTTGAAGAGAGAAAGAAGGAAAAAATGGGACGAACAAAACCAAGAGGAAATTGCTAAGGCTGTTAAGCATCTTCATGAATTTGACCAGAAACACATTAGAGCGGAGGATCCTAACTTGAAAAGGGCTCGGGAGGACCTGCAAAACAGAGTTGATTACCTACAAAAGCAAGCTGATAGCTATGATGACAAAGGGCCTGTCATAGATGCTGTTGTATGGCATGATGGAGAAGTATGGAGGGTTGCCATTGACACACAGACTCTTGAGGATGACCCTGATTGTGGAAAACTCGCTGACTTTGTGCCCCTAACTAATTATAGGATAGAACGAAAGTATGGCGTTTTCAGCAAATTGGATGCCTGTACGTTTGTTGTTAATGTGTATGATGAAGGGAAAATATTGAGTATCGTAACAGATTGCTCTCCCCATGGCACACATGTTGCTGGTATTGCTACTGCTTTCCATGCGAAGGAGCCCCTGTTGAATGGAGTTGCACCTGGAGCACAAGTTATATCTTGTAAAATTGGGGATGCACGCTTAGGTTCAATGGAGACAGGAACTGGGCTGACTCGAGCTTTGATTGCTGCTGTGGAGCATAAATGTGATCTGATCAACATGAGCTATGGAGAACCAGCGTTACTGCCAAACTATGGGCGCTTTGTTGACCTCGTTAATGAAGCTGTGAACAAGCACCGTCTGGTATTTGTGAGTAGTGCCGGCAATAGTGGGCCGGCATTGAGCACTGTTGGGGCACCTGGTGGTACCACATCAAGCATTATAGGAGTTGGTGCATATGTCTCTCCTGCAATGGCTGCTGGTGCTCATTGTGTAGTTGAAGCCCCCGGTGAAGGGCTTGAATACACTTGGTCTAGCCGAGGACCAACTGCCGATGGAGATCTTGGTGTCTGTATTAGTGCTCCTGGTGCAGCTATTGCTCCTGTTCCTACGTGGACCCTCCAACGACGGATGCTCATGAATGGAACATCAATGGCATCCCCATCTGCTTGTGGTGGAATTGCACTACTCATAAGTGCATTAAAGGCTGAGGGTATTTCTGTGAGTCCATATAGTGTAAGGAAGGCTCTTGAAAATACATCTGTTCCTGTAGGCAGTTTACCAGAAGATAAATTATCAACTGGGCAAGGACTTATGCAAGTTGACAAGGCACATGAATACCTAAAGCAGTCTCGGGATGTACCATCTGTCTGGTATCAGATAAAGATAAACCAATCTGGTAAAACAACTCCTACATCACGAGGTATTTACCTAAGGGAGGCTAGTGCTTGCCAACATTCTACCGAGTGGACAGTGCAAGTTGAACCAAAATTTCATGAGGGTGCGAGTAATTTAGAAGAATTGGTTCCTTTTGAGGAGTGTATTGAGTTACATTCCACTGACAATGCAGTTGTGAGGGCTCCTGAGTTTCTTCTTCTTACTCATAATGGCCGTAGCTTCAACATAGTTGTAAATCCTACCAACCTTAGTGAAGGTCTGCACTATTATGAACTATATGGTGTTGATTGCAAAGCACCTTGGCGCGGCCCACTTTTCAGAATCCCAATTACTATAACAAAGCCAATCACTGTCATAAGTCGACCTCCACTATATTCATTTTCGAGGATGTCTTTTTTGCCAGGCCACATAGAAAGGAGATTTATTAAAGTACCTCTTGGTGCCACATGGGTTGAAGCAACCATGCAAACATCAGGATTTGATACAACACGAAGATTTTTTGTTGACTCTGTTCAGCTTTGTCCCTTACAAAGACCTCGTAAGTGGGAGAGTGTTGTAACATTCTCTTCGCCTGCTGCCAAAAGCTTTTCATTTCCTGTTGTGGGTGGTCAGACAATGGAATTAGCTATAGCTCAGTTTTGGTCAAGTGGCATAGGAAGCAACGAAACAACTTGTGTGGATTTTGAGATTGTGTTTCATGGGATTAATGTTAATAAAGAGGAACTAGTACTTGATGGAAGTGAGGCACCAGTAAGAATTGAAGCTGAAGCTCTACTAGCATCCGAGAAACTTGCACCTGCTGCTACTCTAAGCAAGATCAGAATTCCATATCGACCAGTTGCGGCTGAACTTTGTTCTCTTCCAACAGATCGTGACAAACTACCCTCAGAAAAACGTATTTTGGCACTGACATTAACTTACAAGTTCAAATTGGAAGATGGAGCTGAAGTAAAACCTCAAGTTCCATTACTTAACGATCGCATATATGATACTAAATTTGAGTCTCAGTTTTATATGATCTCTGATGCAAACAAGCGTGTATATGCAACGGGTGAAGCTTATCCAAGCTCTTCAAAACTGCCCAAAGGCGAATACACTTTACAACTATATCTTAGGCATGAAAACATGCAATATCTGGAAAAATTGAAGCAGCTGGTTTTGTTCATTGAAAGAAAATTGGAAGAGAAGGATGTAATGCGTTTGAGTTTTTTCTCTCAACCGGATGGCCCTGTGATGGGAAATGGTGCTTATAAGTCCTCAGTTTTAGTTCCAGGGAAAAAAGAAGCAATCTATTTGGGCCCACCATCCAAAGACAAGCTCCTAAAGTTTTCTCCGCAAGGATCTGTTTTGCTTGGAGCTATCTCCTATGGGAAGCTATCATATGTTGAAAAAGGACAAGGGAAAGATCCGCAAAAGAACCCGGTGTCTTATCAGATTTCTTATATTGTGCCACCGAATAAGATGGATGAAGACAAAGGAAAAGGTTCTTCTACTTCCACCAAAGCTGTTTCTGAACGTTTAGAAGAAGAGGTTCGAGATGCAAAGATTAAAGTTCTTACAAGCCTCAAACAAGACAATGAAGAAGAACGCTCAGAGTGGAACAAATTATCTACCTCTCTTAAGTCTGAATACCCAAAACACACCCCACTGCTTGCAAAGATTTTGGAAGGTTTGCTTTCTCGAAACAATGTCGAGGACAAAATCTGTCATGACAAAGAGGTTATTGATGCAGCAAATGAGGTTGTTGATAGTATTGACAGAGATGAACTAGCAAAATTTTTGTCACTTAGAACTGATCCCGAAGATGAGGAGGCAGAGAAAAATAAAAAGAAGATGGAGACCACTCGTGATCAGTTAGCAGAGGCATTGTACCAAAAAGGACTAGCACTGGCTGATATTTTGTCTTTACAGGGGGATAAGCCTTCGGCGTCGGAAGTAACTGAAGGTTCAAAAGAGGCAGACAAGACTGCTGATTCTGGACCAGGCTCTGGTGTCCTGCTTGAATGCTTTGAAGATACTTTTAAAGAACTTCAAAAATGGGTCGAAGTGAAATCCTCTAAATACGGAATCCTCTCAGTAACACGTGAGAAACATCATGGAAGGCTTGGAACAGCACTTAAGGTCTTGAATGACATAATTCAAGAGAATACGGAGCCTCCGAAGAAGAAGCTGTACGAAGAAAAGCTATCTCTGCTTGAAGAGATCGGGTGGCAGCATTTGGTGACATATGAGAGACAATGGATGCATGTGCGCTTTCCACCAAGTTTACCTCTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1377

Amino Acids

152.28

Weight (kDa)

6.46

Isoelectric Point (pI)

40.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	114 - 577	1e-44	Subtilase family
TPPII_Ig-like-1	PF21223	624 - 730	3.1e-22	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	750 - 834	2.1e-15	TPPII, galactose-binding domain-like
TPPII	PF12580	876 - 1060	1.2e-59	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	582, 3105
AarI	CACCTGC	1 cut(s)	2659
Acc36I	ACCTGC	2 cut(s)	768, 2659
Acc65I	GGTACC	1 cut(s)	1382
AccB1I	GGYRCC	5 cut(s)	302, 1372, 1382, 2294, 2599
AccB7I	CCANNNNNTGG	2 cut(s)	1271, 3842
AccBSI	CCGCTC	1 cut(s)	725
AccI	GTMKAC	1 cut(s)	2215
AccII	CGCG	1 cut(s)	2160
AciI	CCGC	9 cut(s)	210, 213, 216, 622, 725, 2160, 2696, 3188, 3263
AclI	AACGTT	1 cut(s)	3370
AclWI	GGATC	5 cut(s)	725, 738, 3202, 3254, 3644
AcoI	YGGCCR	2 cut(s)	340, 2065
AcsI	RAATTY	6 cut(s)	426, 701, 1946, 2676, 2849, 3629
AcuI	CTGAAG	4 cut(s)	501, 2640, 2820, 3816
AcvI	CACGTG	3 cut(s)	50, 574, 3938
AcyI	GRCGYC	3 cut(s)	303, 348, 3782
AdeI	CACNNNGTG	1 cut(s)	1379
AfaI	GTAC	7 cut(s)	985, 1384, 1818, 2286, 2583, 3725, 4028
AflII	CTTAAG	2 cut(s)	3473, 3971
AflIII	ACRYGT	2 cut(s)	1057, 3935
AhlI	ACTAGT	1 cut(s)	2578
AjiI	CACGTC	2 cut(s)	59, 535
AjnI	CCWGG	7 cut(s)	508, 1117, 1375, 1540, 2254, 3124, 3835
Alw21I	GWGCWC	4 cut(s)	1126, 1364, 1444, 1540
Alw26I	GTCTC	9 cut(s)	65, 118, 134, 1166, 1423, 1812, 2862, 3686, 4081
AlwI	GGATC	5 cut(s)	725, 738, 3202, 3254, 3644
AlwNI	CAGNNNCTG	2 cut(s)	1181, 3842
Ama87I	CYCGRG	3 cut(s)	750, 1191, 1809
AoxI	GGCC	9 cut(s)	340, 486, 815, 1349, 2065, 2161, 2257, 3079, 3151
ApaI	GGGCCC	1 cut(s)	3155
ApoI	RAATTY	6 cut(s)	426, 701, 1946, 2676, 2849, 3629
ArsI	GACNNNNNNTTYG	6 cut(s)	804, 836, 1180, 1212, 3620, 3652
AseI	ATTAAT	1 cut(s)	2559
Asp700I	GAANNNNTTC	3 cut(s)	1980, 3337, 3384
Asp718I	GGTACC	1 cut(s)	1382
AspLEI	GCGC	4 cut(s)	305, 1278, 2160, 4107
AsuC2I	CCSGG	3 cut(s)	383, 1464, 3275
AsuHPI	GGTGA	5 cut(s)	493, 575, 1478, 2918, 4087
AvaI	CYCGRG	3 cut(s)	750, 1191, 1809
AvaII	GGWCC	5 cut(s)	379, 757, 1499, 1572, 3833
AxyI	CCTNAGG	1 cut(s)	1889
BaeGI	GKGCMC	3 cut(s)	927, 1375, 3155
BamHI	GGATCC	1 cut(s)	730
BanI	GGYRCC	5 cut(s)	302, 1372, 1382, 2294, 2599
BanII	GRGCYC	4 cut(s)	751, 1098, 2038, 3155
BarI	GAAGNNNNNNTAC	2 cut(s)	4010, 4042
BauI	CACGAG	2 cut(s)	1875, 3699
BbrPI	CACGTG	3 cut(s)	50, 574, 3938
BbsI	GAAGAC	2 cut(s)	136, 3330
Bbv12I	GWGCWC	4 cut(s)	1126, 1364, 1444, 1540
BbvCI	CCTCAGC	1 cut(s)	1665
BceAI	ACGGC	3 cut(s)	327, 355, 2052
BcgI	CGANNNNNNTGC	2 cut(s)	2627, 2661
BciT130I	CCWGG	7 cut(s)	510, 1119, 1377, 1542, 2256, 3126, 3837
BclI	TGATCA	2 cut(s)	1230, 3703
BcnI	CCSGG	3 cut(s)	383, 1464, 3275
BcoDI	GTCTC	9 cut(s)	65, 118, 134, 1166, 1423, 1812, 2862, 3686, 4081
BcuI	ACTAGT	1 cut(s)	2578
BfaI	CTAG	7 cut(s)	431, 1491, 1899, 2579, 2630, 3623, 3737
BfmI	CTRYAG	2 cut(s)	1725, 2481
BfoI	RGCGCY	2 cut(s)	306, 1279
BfrI	CTTAAG	2 cut(s)	3473, 3971
BfuAI	ACCTGC	2 cut(s)	768, 2659
BglII	AGATCT	1 cut(s)	1516
BmcAI	AGTACT	1 cut(s)	2583
Bme18I	GGWCC	5 cut(s)	379, 757, 1499, 1572, 3833
BmeT110I	CYCGRG	3 cut(s)	750, 1191, 1809
BmgBI	CACGTC	2 cut(s)	59, 535
BmrI	ACTGGG	3 cut(s)	459, 1191, 1767
BmuI	ACTGGG	3 cut(s)	459, 1191, 1767
BoxI	GACNNNNGTC	1 cut(s)	57
BpiI	GAAGAC	2 cut(s)	136, 3330
BpmI	CTGGAG	3 cut(s)	393, 531, 1140
Bpu10I	CCTNAGC	3 cut(s)	678, 1159, 1665
BpuEI	CTTGAG	2 cut(s)	905, 2796
BpuMI	CCSGG	3 cut(s)	383, 1464, 3275
Bsa29I	ATCGAT	1 cut(s)	310
BsaAI	YACGTR	4 cut(s)	50, 574, 1569, 3938
BsaBI	GATNNNNATC	1 cut(s)	1515
BsaHI	GRCGYC	3 cut(s)	303, 348, 3782
BsaI	GGTCTC	2 cut(s)	134, 3686
BsaJI	CCNNGG	6 cut(s)	343, 1048, 1462, 1495, 2153, 3125
BsaWI	WCCGGW	1 cut(s)	3073
Bse118I	RCCGGY	2 cut(s)	1337, 1351
Bse1I	ACTGG	7 cut(s)	168, 454, 1186, 1762, 2603, 2690, 3747
Bse21I	CCTNAGG	1 cut(s)	1889
Bse3DI	GCAATG	2 cut(s)	195, 1434
Bse8I	GATNNNNATC	1 cut(s)	1515
BseBI	CCWGG	7 cut(s)	510, 1119, 1377, 1542, 2256, 3126, 3837
BseCI	ATCGAT	1 cut(s)	310
BseDI	CCNNGG	6 cut(s)	343, 1048, 1462, 1495, 2153, 3125
BseJI	GATNNNNATC	1 cut(s)	1515
BseMI	GCAATG	2 cut(s)	195, 1434
BseMII	CTCAG	8 cut(s)	1656, 2031, 2501, 2754, 2873, 3126, 3459, 3942
BseNI	ACTGG	7 cut(s)	168, 454, 1186, 1762, 2603, 2690, 3747
BseRI	GAGGAG	3 cut(s)	53, 2006, 3677
BseSI	GKGCMC	3 cut(s)	927, 1375, 3155
BseX3I	CGGCCG	1 cut(s)	340
BsgI	GTGCAG	4 cut(s)	430, 515, 1566, 2099
Bsh1236I	CGCG	1 cut(s)	2160
Bsh1285I	CGRYCG	3 cut(s)	310, 343, 2833
BshFI	GGCC	9 cut(s)	342, 488, 817, 1351, 2067, 2163, 2259, 3081, 3153
BshNI	GGYRCC	5 cut(s)	302, 1372, 1382, 2294, 2599
BshVI	ATCGAT	1 cut(s)	310
BsiEI	CGRYCG	3 cut(s)	310, 343, 2833
BsiHKAI	GWGCWC	4 cut(s)	1126, 1364, 1444, 1540
BsiHKCI	CYCGRG	3 cut(s)	750, 1191, 1809
BsiSI	CCGG	6 cut(s)	383, 1338, 1352, 1464, 3074, 3275
BslFI	GGGAC	2 cut(s)	665, 2361
BsmAI	GTCTC	9 cut(s)	65, 118, 134, 1166, 1423, 1812, 2862, 3686, 4081
BsmBI	CGTCTC	2 cut(s)	65, 118
BsmFI	GGGAC	2 cut(s)	665, 2361
BsmI	GAATGC	1 cut(s)	3863
BsnI	GGCC	9 cut(s)	342, 488, 817, 1351, 2067, 2163, 2259, 3081, 3153
Bso31I	GGTCTC	2 cut(s)	134, 3686
BsoBI	CYCGRG	3 cut(s)	750, 1191, 1809
Bsp120I	GGGCCC	1 cut(s)	3151
Bsp19I	CCATGG	1 cut(s)	1048
BspACI	CCGC	9 cut(s)	210, 213, 216, 622, 725, 2160, 2696, 3188, 3263
BspANI	GGCC	9 cut(s)	342, 488, 817, 1351, 2067, 2163, 2259, 3081, 3153
BspCNI	CTCAG	8 cut(s)	1657, 2032, 2500, 2753, 2872, 3125, 3458, 3941
BspDI	ATCGAT	1 cut(s)	310
BspFNI	CGCG	1 cut(s)	2160
BspHI	TCATGA	4 cut(s)	697, 1593, 1951, 3562
BspMI	ACCTGC	2 cut(s)	768, 2659
BspPI	GGATC	5 cut(s)	725, 738, 3202, 3254, 3644
BspQI	GCTCTTC	1 cut(s)	2929
BspT107I	GGYRCC	5 cut(s)	302, 1372, 1382, 2294, 2599
BspTI	CTTAAG	2 cut(s)	3473, 3971
BspTNI	GGTCTC	2 cut(s)	134, 3686
BsrBI	CCGCTC	1 cut(s)	725
BsrDI	GCAATG	2 cut(s)	195, 1434
BsrFI	RCCGGY	2 cut(s)	1337, 1351
BsrI	ACTGG	7 cut(s)	168, 454, 1186, 1762, 2603, 2690, 3747
BssAI	RCCGGY	2 cut(s)	1337, 1351
BssECI	CCNNGG	6 cut(s)	343, 1048, 1462, 1495, 2153, 3125
BssNI	GRCGYC	3 cut(s)	303, 348, 3782
BssSI	CACGAG	2 cut(s)	1875, 3699
BssT1I	CCWWGG	2 cut(s)	1048, 2153
Bst2BI	CACGAG	2 cut(s)	1875, 3699
Bst2UI	CCWGG	7 cut(s)	510, 1119, 1377, 1542, 2256, 3126, 3837
Bst4CI	ACNGT	6 cut(s)	227, 500, 1316, 1366, 1930, 2206
Bst6I	CTCTTC	8 cut(s)	547, 623, 2425, 2718, 2929, 3030, 3375, 4047
BstACI	GRCGYC	3 cut(s)	303, 348, 3782
BstAFI	CTTAAG	2 cut(s)	3473, 3971
BstAPI	GCANNNNNTGC	3 cut(s)	1433, 1553, 2654
BstBAI	YACGTR	4 cut(s)	50, 574, 1569, 3938
BstC8I	GCNNGC	7 cut(s)	792, 1156, 1339, 1353, 1906, 2427, 3507
BstDSI	CCRYGG	2 cut(s)	343, 1048
BstENI	CCTNNNNNAGG	1 cut(s)	1892
BstFNI	CGCG	1 cut(s)	2160
BstH2I	RGCGCY	2 cut(s)	306, 1279
BstHHI	GCGC	4 cut(s)	305, 1278, 2160, 4107
BstMAI	GTCTC	9 cut(s)	65, 118, 134, 1166, 1423, 1812, 2862, 3686, 4081
BstMCI	CGRYCG	3 cut(s)	310, 343, 2833
BstNI	CCWGG	7 cut(s)	510, 1119, 1377, 1542, 2256, 3126, 3837
BstNSI	RCATGY	3 cut(s)	1061, 2983, 4103
BstPAI	GACNNNNGTC	1 cut(s)	57
BstSFI	CTRYAG	2 cut(s)	1725, 2481
BstSLI	GKGCMC	3 cut(s)	927, 1375, 3155
BstUI	CGCG	1 cut(s)	2160
BstV2I	GAAGAC	2 cut(s)	136, 3330
BstX2I	RGATCY	4 cut(s)	730, 1516, 3194, 3259
BstXI	CCANNNNNNTGG	1 cut(s)	1827
BstYI	RGATCY	4 cut(s)	730, 1516, 3194, 3259
BstZI	CGGCCG	1 cut(s)	340
Bsu15I	ATCGAT	1 cut(s)	310
Bsu36I	CCTNAGG	1 cut(s)	1889
BsuRI	GGCC	9 cut(s)	342, 488, 817, 1351, 2067, 2163, 2259, 3081, 3153
BsuTUI	ATCGAT	1 cut(s)	310
BtgI	CCRYGG	2 cut(s)	343, 1048
BtrI	CACGTC	2 cut(s)	59, 535
BtsI	GCAGTG	1 cut(s)	3500
BtsIMutI	CAGTG	8 cut(s)	447, 1362, 1935, 2013, 2202, 2759, 3500, 3740
BveI	ACCTGC	2 cut(s)	768, 2659
Cac8I	GCNNGC	7 cut(s)	792, 1156, 1339, 1353, 1906, 2427, 3507
CaiI	CAGNNNCTG	2 cut(s)	1181, 3842
CciI	TCATGA	4 cut(s)	697, 1593, 1951, 3562
CfoI	GCGC	4 cut(s)	305, 1278, 2160, 4107
Cfr10I	RCCGGY	2 cut(s)	1337, 1351
ClaI	ATCGAT	1 cut(s)	310
CseI	GACGC	2 cut(s)	337, 3771
CsiI	ACCWGGT	1 cut(s)	1375
Csp6I	GTAC	7 cut(s)	984, 1383, 1817, 2285, 2582, 3724, 4027
CspCI	CAANNNNNGTGG	4 cut(s)	2287, 2322, 2511, 2546
CviQI	GTAC	7 cut(s)	984, 1383, 1817, 2285, 2582, 3724, 4027
DinI	GGCGCC	1 cut(s)	304
DraI	TTTAAA	1 cut(s)	3877
DraIII	CACNNNGTG	1 cut(s)	1379
EaeI	YGGCCR	2 cut(s)	340, 2065
EagI	CGGCCG	1 cut(s)	340
Eam1104I	CTCTTC	8 cut(s)	547, 623, 2425, 2718, 2929, 3030, 3375, 4047
EarI	CTCTTC	8 cut(s)	547, 623, 2425, 2718, 2929, 3030, 3375, 4047
EclXI	CGGCCG	1 cut(s)	340
Eco130I	CCWWGG	2 cut(s)	1048, 2153
Eco24I	GRGCYC	4 cut(s)	751, 1098, 2038, 3155
Eco31I	GGTCTC	2 cut(s)	134, 3686
Eco47I	GGWCC	5 cut(s)	379, 757, 1499, 1572, 3833
Eco52I	CGGCCG	1 cut(s)	340
Eco57I	CTGAAG	4 cut(s)	501, 2640, 2820, 3816
Eco72I	CACGTG	3 cut(s)	50, 574, 3938
Eco81I	CCTNAGG	1 cut(s)	1889
Eco88I	CYCGRG	3 cut(s)	750, 1191, 1809
EcoNI	CCTNNNNNAGG	1 cut(s)	1892
EcoO109I	RGGNCCY	2 cut(s)	757, 815
EcoRI	GAATTC	1 cut(s)	2676
EcoRII	CCWGG	7 cut(s)	508, 1117, 1375, 1540, 2254, 3124, 3835
EcoT14I	CCWWGG	2 cut(s)	1048, 2153
EcoT22I	ATGCAT	1 cut(s)	4101
EcoT38I	GRGCYC	4 cut(s)	751, 1098, 2038, 3155
EgeI	GGCGCC	1 cut(s)	304
EheI	GGCGCC	1 cut(s)	304
ErhI	CCWWGG	2 cut(s)	1048, 2153
Esp3I	CGTCTC	2 cut(s)	65, 118
FalI	AAGNNNNNCTT	4 cut(s)	2274, 2306, 3513, 3545
FaqI	GGGAC	2 cut(s)	665, 2361
FauI	CCCGC	1 cut(s)	629
FauNDI	CATATG	3 cut(s)	1414, 3235, 4081
FbaI	TGATCA	2 cut(s)	1230, 3703
FblI	GTMKAC	1 cut(s)	2215
FriOI	GRGCYC	4 cut(s)	751, 1098, 2038, 3155
FspBI	CTAG	7 cut(s)	431, 1491, 1899, 2579, 2630, 3623, 3737
GlaI	GCGC	4 cut(s)	304, 1277, 2159, 4106
GsuI	CTGGAG	3 cut(s)	393, 531, 1140
HaeII	RGCGCY	2 cut(s)	306, 1279
HaeIII	GGCC	9 cut(s)	342, 488, 817, 1351, 2067, 2163, 2259, 3081, 3153
HapII	CCGG	6 cut(s)	383, 1338, 1352, 1464, 3074, 3275
HgaI	GACGC	2 cut(s)	337, 3771
HhaI	GCGC	4 cut(s)	305, 1278, 2160, 4107
Hin1I	GRCGYC	3 cut(s)	303, 348, 3782
Hin6I	GCGC	4 cut(s)	303, 1276, 2158, 4105
HinP1I	GCGC	4 cut(s)	303, 1276, 2158, 4105
HincII	GTYRAC	5 cut(s)	538, 1285, 1780, 2216, 2359
HindII	GTYRAC	5 cut(s)	538, 1285, 1780, 2216, 2359
HindIII	AAGCTT	2 cut(s)	2437, 2910
HpaII	CCGG	6 cut(s)	383, 1338, 1352, 1464, 3074, 3275
HphI	GGTGA	5 cut(s)	493, 575, 1478, 2918, 4087
Hpy99I	CGWCG	7 cut(s)	17, 227, 320, 341, 353, 1587, 3787
HpyCH4III	ACNGT	6 cut(s)	227, 500, 1316, 1366, 1930, 2206
Hsp92I	GRCGYC	3 cut(s)	303, 348, 3782
HspAI	GCGC	4 cut(s)	303, 1276, 2158, 4105
KasI	GGCGCC	1 cut(s)	302
KpnI	GGTACC	1 cut(s)	1386
KroI	GCCGGC	2 cut(s)	1337, 1351
KroNI	GCCGGC	2 cut(s)	1339, 1353
Ksp22I	TGATCA	2 cut(s)	1230, 3703
LguI	GCTCTTC	1 cut(s)	2929
MabI	ACCWGGT	1 cut(s)	1375
MaeI	CTAG	7 cut(s)	431, 1491, 1899, 2579, 2630, 3623, 3737
MbiI	CCGCTC	1 cut(s)	725
MflI	RGATCY	4 cut(s)	730, 1516, 3194, 3259
Mly113I	GGCGCC	1 cut(s)	303
MlyI	GAGTC	7 cut(s)	47, 269, 874, 1183, 1690, 2354, 2864
MmeI	TCCRAC	3 cut(s)	1603, 2741, 3765
Mph1103I	ATGCAT	1 cut(s)	4101
MroNI	GCCGGC	2 cut(s)	1337, 1351
MroXI	GAANNNNTTC	3 cut(s)	1980, 3337, 3384
MslI	CAYNNNNRTG	4 cut(s)	696, 1221, 1956, 2121
MspA1I	CMGCKG	1 cut(s)	3007
MspCI	CTTAAG	2 cut(s)	3473, 3971
MspI	CCGG	6 cut(s)	383, 1338, 1352, 1464, 3074, 3275
Mva1269I	GAATGC	1 cut(s)	3863
MvaI	CCWGG	7 cut(s)	510, 1119, 1377, 1542, 2256, 3126, 3837
MvnI	CGCG	1 cut(s)	2160
NaeI	GCCGGC	2 cut(s)	1339, 1353
NarI	GGCGCC	1 cut(s)	303
NciI	CCSGG	3 cut(s)	383, 1464, 3275
NcoI	CCATGG	1 cut(s)	1048
NdeI	CATATG	3 cut(s)	1414, 3235, 4081
NgoMIV	GCCGGC	2 cut(s)	1337, 1351
NmeAIII	GCCGAG	2 cut(s)	138, 1520
NmuCI	GTSAC	5 cut(s)	45, 50, 2726, 3636, 4075
NsiI	ATGCAT	1 cut(s)	4101
NspI	RCATGY	3 cut(s)	1061, 2983, 4103
PaeR7I	CTCGAG	1 cut(s)	1191
PagI	TCATGA	4 cut(s)	697, 1593, 1951, 3562
PaqCI	CACCTGC	1 cut(s)	2659
PciI	ACATGT	1 cut(s)	1057
PciSI	GCTCTTC	1 cut(s)	2929
PcsI	WCGNNNNNNNCGW	4 cut(s)	55, 284, 345, 4010
PctI	GAATGC	1 cut(s)	3863
PdiI	GCCGGC	2 cut(s)	1339, 1353
PdmI	GAANNNNTTC	3 cut(s)	1980, 3337, 3384
PfeI	GAWTC	5 cut(s)	312, 368, 2175, 3827, 3921
PflMI	CCANNNNNTGG	2 cut(s)	1271, 3842
Ple19I	CGATCG	2 cut(s)	310, 2833
PleI	GAGTC	7 cut(s)	47, 268, 874, 1183, 1689, 2354, 2863
PluTI	GGCGCC	1 cut(s)	306
PmaCI	CACGTG	3 cut(s)	50, 574, 3938
PmlI	CACGTG	3 cut(s)	50, 574, 3938
PpsI	GAGTC	7 cut(s)	47, 268, 874, 1183, 1689, 2354, 2863
Ppu21I	YACGTR	4 cut(s)	50, 574, 1569, 3938
PpuMI	RGGWCCY	1 cut(s)	757
PscI	ACATGT	1 cut(s)	1057
PshAI	GACNNNNGTC	1 cut(s)	57
PshBI	ATTAAT	1 cut(s)	2559
PsiI	TTATAA	2 cut(s)	582, 3105
Psp1406I	AACGTT	1 cut(s)	3370
Psp5II	RGGWCCY	1 cut(s)	757
Psp6I	CCWGG	7 cut(s)	508, 1117, 1375, 1540, 2254, 3124, 3835
PspCI	CACGTG	3 cut(s)	50, 574, 3938
PspGI	CCWGG	7 cut(s)	508, 1117, 1375, 1540, 2254, 3124, 3835
PspOMI	GGGCCC	1 cut(s)	3151
PspPPI	RGGWCCY	1 cut(s)	757
PspXI	VCTCGAGB	1 cut(s)	1191
PsrI	GAACNNNNNNTAC	2 cut(s)	1242, 1274
PstNI	CAGNNNCTG	2 cut(s)	1181, 3842
PsuI	RGATCY	4 cut(s)	730, 1516, 3194, 3259
PvuI	CGATCG	2 cut(s)	310, 2833
PvuII	CAGCTG	1 cut(s)	3007
RsaI	GTAC	7 cut(s)	985, 1384, 1818, 2286, 2583, 3725, 4028
RsaNI	GTAC	7 cut(s)	984, 1383, 1817, 2285, 2582, 3724, 4027
RseI	CAYNNNNRTG	4 cut(s)	696, 1221, 1956, 2121
SalI	GTCGAC	1 cut(s)	2214
SapI	GCTCTTC	1 cut(s)	2929
ScaI	AGTACT	1 cut(s)	2583
SchI	GAGTC	7 cut(s)	47, 269, 874, 1183, 1690, 2354, 2864
SexAI	ACCWGGT	1 cut(s)	1375
SfcI	CTRYAG	2 cut(s)	1725, 2481
SfoI	GGCGCC	1 cut(s)	304
Sfr274I	CTCGAG	1 cut(s)	1191
SinI	GGWCC	5 cut(s)	379, 757, 1499, 1572, 3833
SlaI	CTCGAG	1 cut(s)	1191
SmiMI	CAYNNNNRTG	4 cut(s)	696, 1221, 1956, 2121
SmlI	CTYRAG	5 cut(s)	884, 1191, 2811, 3473, 3971
SmoI	CTYRAG	5 cut(s)	884, 1191, 2811, 3473, 3971
SpeI	ACTAGT	1 cut(s)	2578
SsiI	CCGC	9 cut(s)	210, 213, 216, 622, 725, 2160, 2696, 3188, 3263
SspDI	GGCGCC	1 cut(s)	302
SspI	AATATT	1 cut(s)	1020
SspMI	CTAG	7 cut(s)	431, 1491, 1899, 2579, 2630, 3623, 3737
StyI	CCWWGG	2 cut(s)	1048, 2153
TaaI	ACNGT	6 cut(s)	227, 500, 1316, 1366, 1930, 2206
TaqII	GACCGA	1 cut(s)	158
TatI	WGTACW	1 cut(s)	2581
TauI	GCSGC	4 cut(s)	213, 216, 2163, 2699
TfiI	GAWTC	5 cut(s)	312, 368, 2175, 3827, 3921
TscAI	CASTG	8 cut(s)	454, 1369, 1935, 2020, 2209, 2766, 3507, 3747
TseFI	GTSAC	5 cut(s)	45, 50, 2726, 3636, 4075
Tsp45I	GTSAC	5 cut(s)	45, 50, 2726, 3636, 4075
TspGWI	ACGGA	3 cut(s)	1600, 3933, 4019
TspRI	CASTG	8 cut(s)	454, 1369, 1935, 2020, 2209, 2766, 3507, 3747
Van91I	CCANNNNNTGG	2 cut(s)	1271, 3842
Vha464I	CTTAAG	2 cut(s)	3473, 3971
VpaK11BI	GGWCC	5 cut(s)	379, 757, 1499, 1572, 3833
VspI	ATTAAT	1 cut(s)	2559
XagI	CCTNNNNNAGG	1 cut(s)	1892
XapI	RAATTY	6 cut(s)	426, 701, 1946, 2676, 2849, 3629
XbaI	TCTAGA	1 cut(s)	430
XceI	RCATGY	3 cut(s)	1061, 2983, 4103
XhoI	CTCGAG	1 cut(s)	1191
XmiI	GTMKAC	1 cut(s)	2215
XmnI	GAANNNNTTC	3 cut(s)	1980, 3337, 3384
XspI	CTAG	7 cut(s)	431, 1491, 1899, 2579, 2630, 3623, 3737
ZrmI	AGTACT	1 cut(s)	2583
Zsp2I	ATGCAT	1 cut(s)	4101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.