Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Forward (+)

3504268 .. 3516625

Gene Structure 12358 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00281150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3807 bp

ATGCCTTGCTCTGCGATTAGCGGCGGCGGTAGCGACGGTAATGGTTCTCTGCGTAATTTCAAGCTCAACGAGTCCACGTTCTTAGCCTCGCTCATGCCGAAGAAGGAGATCGGCGCCGATCGATTCGTCGAGGCTCATCCGAATTACGACGGCCGCGGCGTCGTTATTGCTATCTTCGATTCTGGAGTGGACCCGGCTGCTGCTGGATTACAGGTTACGTCAGATGGAAAACCGAAAATTCTAGATGTTCTTGACTGCACTGGGAGTGGAGATGTTGATACTTCAAAGGTGGTGAAGGCCGATGACAACGGTTGCATACCTGGAGCTTCAGGGGCATGTCTGCACGTCAACTCTTCATGGAAAAATCCTTCTGGTGAATGGCACGTGGGTTATAAGTTGGTTTACGAGCTATTTACTGACACATTGACTTCCCGCTTGAAGAGAGAAAGAAGGAAAAAATGGGACGAACAAAACCAAGAGGAAATTGCTAAGGCTGTTAAGCATCTTCATGAATTTGACCAGAAACACATTAGAGTGGAGGATGCTAACTTGAAAAGGGCTCGGGAGGACCTGCAAAACAGAGTTGATTACCTACAAAAGCAAGCTGATAGCTATGATGACAAAGGGCCTGTCATAGATGCTGTTGTATGGCATGATGGAGAAGTATGGAGGGTTGCCATTGACACACAGACTCTTGAGGATGACCCTGATTGTGGAAAACTCGCTGACTTTGTGCCCCTAACTAATTATAGGATAGAACGAAAGTATGGCGTTTTCAGCAAATTGGATGCCTGTACGTTTGTTGTTAATGTGTATGATGAAGGGAAGATATTGAGTATCGTAACAGATTGCTCTCCCCATGGCACACATGTTGCTGGTATTGCTACTGCTTTCCATGCGAAGGAGCCCCTGTTGAATGGAGTTGCACCTGGAGCACAAGTTATATCTTGTAAAATTGGGGATGCACGCTTAGGTTCAATGGAGACAGGAACTGGGCTGACTCGAGCTTTGATTGCTGCTGTGGAGCATAAATGTGATCTGATCAACATGAGCTATGGAGAACCAGCGTTACTGCCAAACTATGGGCGCTTTGTTGACCTCGTTAATGAAGCTGTGAACAAGCACCGTCTGGTATTTGTGAGTAGTGCCGGCAATAGTGGGCCGGCATTGAGCACTGTTGGGGCACCTGGTGGTACCACATCAAGCATTATAGGAGTTGGTGCATATGTATCTCCTGCAATGGCTGCTGGTGCTCATTGTGTAGTTGAAGCCCCCGGTGAAGGTCTTGAATACACTTGGTCTAGCCGAGGACCAACTGCCGATGGAGATCTTGGTGTCTGTATTAGTGCTCCCGGTGCAGCTATTGCTCCTGTTCCTACGTGGACCCTCCAACGACGGATGCTCATGAATGGAACATCAATGGCATCCCCATCTGCTTGTGGTGGAATTGCACTACTCATAAGTGCATTGAAGGCTGAGGGTATTTCTGTAAGTCCATATAGTGTAAGGAAGGCTCTTGAAAATACATCTGTTCCTGTAGGCAGTTTGCCAGAAGATAAATTATCAACTGGGCAAGGACTTATGCAAGTTGACAAGGCACATGAATACCTAAAGCAGTCTCGGGATGTACCATCTGTCTGGTATCAGATAAAGATAAACCAATCTGGTAAAACAACTCCTACATCACGAGGTATTTACTTAAGGGAGGCTAGTGCTTGCCAACAATCTACCGAGTGGACAGTGCAAGTTGAACCAAAATTTCATGAGGGTGCGAGTAATTTAGAAGAATTGGTTCCTTTTGAGGAGTGTATTGAGTTACATTCCACTGACAACGCAGTTGTGAGGGCTCCTGAGTTTCTCCTTCTTACTCATAATGGCCGTAGCTTCAACATAGTTGTAAATCCTACCAACCTTAGTGAAGGTCTGCACTATTATGAACTACATGGTGTTGATTGCAAAGCACCTTGGCGTGGCCCTCTTTTTAGAATCCCAATTACTATAACAAAGCCAATCACTGTCATAAGTCGACCTCCACTATATTCATTTTCGAGGATGTCTTTTTTGCCAGGCCACATAGAAAGAAGATTTATTGAAGTACCTCTTGGTGCTACATGGGTTGAAGCAACCATGCAAACATCAGGATTTGATACAACACGAAGATTTTTTGTTGACTCTGTTCAGCTTTGTCCCTTACAAAGACCTCGTAAGTGGGAGAGTGTTGTAACATTCTCTTCGCCTGCTGCCAAAAGCTTTTCATTTCCTGTTGTGGGTGGTCAAACAATGGAATTAGCTATAGCTCAGTTTTGGTCAAGTGGCATAGGAAGCAACGAAACAACTTGTGTGGATTTTGAGATTGTGTTTCATGGGATTAATGTTAATAAAGAGGAACTAGTGCTTGATGGAAGTGAGGCACCAGTAAGAATTGAAGCTGAAGCTCTACTAGCATCTGAGAAACTTGCACCTGCTGCTACTCTAAGCAAGATCAGAATTCCATATCGACCAGTTGCGGCTGAACTTTGTTCTCTTCCAACAGATCGTGACAAACTACCCTCAGAAAAACGTATTTTGGCGCTGACATTAACTTACAAGTTCAAATTGGAAGATGGAGCTGAAGTAAAACCTCAAGTTCCATTACTTAACGATCGCATATATGATACTAAATTTGAGTCTCAGTTTTATATGATCTCTGATGCAAATAAGCGTGTATATGCAACGGGTGAAGCTTATCCAAGCTCTTCAAAACTGCCCAAAGGCGAATACACTTTACAGCTATATCTTAGGCACGAAAACATGCAATATCTGGAAAAATTGAAGCAGCTGGTTTTGTTCATTGAAAGAAAATTGGAAGAGAAGTTTTCTCCGCAAGGATCTGTTTTGCTTGGAGCTATCTCCTATGGGAAGCTATCATATGTTGAAAAAGGACAAGGGAAAGATCCGCAAAAGAACCCGGTGTCTTATCAGATTTCTTATATTGTGCCACCGAATAAGATGGATGAAGACAAAGGAAAAGGTTCTTCTACTTCCACCAAAGCTGTTTCTGAACGTTTAGAAGAAGAGGTTCGAGATGCGAAGATTAAAGTTCTTACAAGCCTCAAACAAGACAATGAAGAAGAACGCTCAGAGTGGAACAAATTATCTACCTCTCTTAAGTCTGAATACCCAAAACACACCCCATTGCTTGCAAAGATTTTGGAAGGTTTGCTTTCTCGAAACAATGTCGAGGACAAAATCTGTCATGACAAAGAGGTTATTGATGCAGCAAATGAGGTTGTTGATAGTATTGACAGAGATGAGCTAGCGAAATTTTTTTCACTTAGAACTGATCCCGAAGATGAGGAGGCAGAGAAAAATAAAAAGAAGATGGAGACGACTCGTGATCAGTTAGCAGAGGCATTGTACCAAAAAGGACTAGCACTGGCAGATATTTTGTCTTTACAGGGGGATAAGCCTTCGGCATCGGAAGTAACTGAAGTTTCGAAAGAGGCAGACAAGACTGCTGATTCTGGACCAGGCTCTGGTGTCCTGCTTGAACGCTTTGAAGACACTTTTAAAGAACTTCAAAAATGGGTCGACGTGAAATCCTCTAAATACGGAATCCTCTCAGTAACACGTGAGAAACATCATGGAAGGCTTGGAACAGCACTTAAGGTCTTGAATGACATAATTCAAGAGAATACGGAGCCTCCGAAGAAGAAGCTGTACGAAGAAAAGCTATCTCTGCTTGAAGAGATCGAGTGGCAGCACTTGGTGACATATGAGAGACAATGGATGCATGTGCGCTTTCCACCAAGTTTACCTCTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1268

Amino Acids

139.97

Weight (kDa)

5.68

Isoelectric Point (pI)

40.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	51 - 514	8.3e-45	Subtilase family
TPPII_Ig-like-1	PF21223	561 - 667	9.1e-23	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	687 - 771	1.9e-15	TPPII, galactose-binding domain-like
TPPII	PF12580	813 - 951	4.3e-51	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	393
AarI	CACCTGC	1 cut(s)	2470
Acc36I	ACCTGC	2 cut(s)	579, 2470
Acc65I	GGTACC	1 cut(s)	1193
AccB1I	GGYRCC	4 cut(s)	113, 1183, 1193, 2410
AccB7I	CCANNNNNTGG	2 cut(s)	1082, 3515
AccI	GTMKAC	2 cut(s)	2026, 3570
AccII	CGCG	1 cut(s)	156
AciI	CCGC	9 cut(s)	21, 24, 27, 154, 156, 433, 2507, 2861, 2936
AclI	AACGTT	1 cut(s)	3043
AclWI	GGATC	3 cut(s)	2875, 2927, 3317
AcoI	YGGCCR	2 cut(s)	151, 1876
AcsI	RAATTY	6 cut(s)	237, 512, 1757, 2487, 2660, 3302
AcuI	CTGAAG	4 cut(s)	312, 2451, 2631, 3489
AcvI	CACGTG	2 cut(s)	385, 3611
AcyI	GRCGYC	2 cut(s)	114, 159
AdeI	CACNNNGTG	2 cut(s)	1190, 3748
AfaI	GTAC	6 cut(s)	796, 1195, 1629, 2097, 3398, 3701
AfiI	CCNNNNNNNGG	7 cut(s)	713, 901, 1082, 1280, 1970, 2267, 3515
AflII	CTTAAG	3 cut(s)	1699, 3146, 3644
AflIII	ACRYGT	2 cut(s)	868, 3608
AhlI	ACTAGT	1 cut(s)	2389
AjiI	CACGTC	2 cut(s)	346, 3574
AjnI	CCWGG	5 cut(s)	319, 928, 1186, 2065, 3508
AjuI	GAANNNNNNNTTGG	2 cut(s)	2085, 2117
Alw21I	GWGCWC	4 cut(s)	937, 1175, 1255, 1351
Alw26I	GTCTC	5 cut(s)	977, 1623, 2673, 3359, 3754
AlwI	GGATC	3 cut(s)	2875, 2927, 3317
AlwNI	CAGNNNCTG	2 cut(s)	992, 3515
Ama87I	CYCGRG	3 cut(s)	561, 1002, 1620
AoxI	GGCC	7 cut(s)	151, 297, 626, 1160, 1876, 1972, 2068
ApoI	RAATTY	6 cut(s)	237, 512, 1757, 2487, 2660, 3302
ArsI	GACNNNNNNTTYG	4 cut(s)	615, 647, 991, 1023
AseI	ATTAAT	1 cut(s)	2370
Asp700I	GAANNNNTTC	3 cut(s)	1791, 3010, 3057
Asp718I	GGTACC	1 cut(s)	1193
AspLEI	GCGC	4 cut(s)	116, 1089, 2572, 3780
AspS9I	GGNCC	8 cut(s)	190, 568, 626, 1160, 1310, 1383, 1973, 3506
AsuC2I	CCSGG	4 cut(s)	194, 1275, 1353, 2948
AsuHPI	GGTGA	5 cut(s)	304, 386, 1289, 2729, 3760
AsuII	TTCGAA	1 cut(s)	3476
AsuNHI	GCTAGC	1 cut(s)	3295
AvaI	CYCGRG	3 cut(s)	561, 1002, 1620
AvaII	GGWCC	5 cut(s)	190, 568, 1310, 1383, 3506
BaeGI	GKGCMC	2 cut(s)	738, 1186
BanI	GGYRCC	4 cut(s)	113, 1183, 1193, 2410
BanII	GRGCYC	3 cut(s)	562, 909, 1849
BarI	GAAGNNNNNNTAC	2 cut(s)	3683, 3715
BauI	CACGAG	2 cut(s)	1686, 3372
BbrPI	CACGTG	2 cut(s)	385, 3611
BbsI	GAAGAC	2 cut(s)	3003, 3546
Bbv12I	GWGCWC	4 cut(s)	937, 1175, 1255, 1351
BbvCI	CCTCAGC	1 cut(s)	1476
BccI	CCATC	9 cut(s)	218, 650, 1316, 1438, 1639, 2393, 2597, 2983, 3355
BceAI	ACGGC	2 cut(s)	166, 1863
BciT130I	CCWGG	5 cut(s)	321, 930, 1188, 2067, 3510
BclI	TGATCA	2 cut(s)	1041, 3376
BcnI	CCSGG	4 cut(s)	194, 1275, 1353, 2948
BcoDI	GTCTC	5 cut(s)	977, 1623, 2673, 3359, 3754
BcuI	ACTAGT	1 cut(s)	2389
BfaI	CTAG	7 cut(s)	242, 1302, 1710, 2390, 2441, 3296, 3410
BfmI	CTRYAG	2 cut(s)	1536, 2292
BfoI	RGCGCY	3 cut(s)	117, 1090, 2573
BfrI	CTTAAG	3 cut(s)	1699, 3146, 3644
BfuAI	ACCTGC	2 cut(s)	579, 2470
BglII	AGATCT	1 cut(s)	1327
Bme1390I	CCNGG	9 cut(s)	194, 321, 930, 1188, 1275, 1353, 2067, 2948, 3510
Bme18I	GGWCC	5 cut(s)	190, 568, 1310, 1383, 3506
BmeT110I	CYCGRG	3 cut(s)	561, 1002, 1620
BmgBI	CACGTC	2 cut(s)	346, 3574
BmgT120I	GGNCC	8 cut(s)	190, 568, 626, 1160, 1310, 1383, 1973, 3506
BmrFI	CCNGG	9 cut(s)	194, 321, 930, 1188, 1275, 1353, 2067, 2948, 3510
BmrI	ACTGGG	3 cut(s)	270, 1002, 1578
BmtI	GCTAGC	1 cut(s)	3299
BmuI	ACTGGG	3 cut(s)	270, 1002, 1578
BpiI	GAAGAC	2 cut(s)	3003, 3546
BpmI	CTGGAG	3 cut(s)	204, 342, 951
Bpu10I	CCTNAGC	3 cut(s)	489, 970, 1476
Bpu14I	TTCGAA	1 cut(s)	3476
BpuEI	CTTGAG	2 cut(s)	716, 2607
BpuMI	CCSGG	4 cut(s)	194, 1275, 1353, 2948
Bsa29I	ATCGAT	1 cut(s)	121
BsaAI	YACGTR	3 cut(s)	385, 1380, 3611
BsaBI	GATNNNNATC	1 cut(s)	1326
BsaHI	GRCGYC	2 cut(s)	114, 159
BsaJI	CCNNGG	5 cut(s)	154, 859, 1273, 1306, 1964
BsaXI	ACNNNNNCTCC	8 cut(s)	896, 924, 926, 954, 2598, 2628, 3667, 3697
Bsc4I	CCNNNNNNNGG	7 cut(s)	713, 901, 1082, 1280, 1970, 2267, 3515
Bse118I	RCCGGY	2 cut(s)	1148, 1162
Bse1I	ACTGG	6 cut(s)	265, 997, 1573, 2414, 2501, 3420
Bse3DI	GCAATG	2 cut(s)	1245, 3173
Bse8I	GATNNNNATC	1 cut(s)	1326
BseBI	CCWGG	5 cut(s)	321, 930, 1188, 2067, 3510
BseCI	ATCGAT	1 cut(s)	121
BseDI	CCNNGG	5 cut(s)	154, 859, 1273, 1306, 1964
BseJI	GATNNNNATC	1 cut(s)	1326
BseLI	CCNNNNNNNGG	7 cut(s)	713, 901, 1082, 1280, 1970, 2267, 3515
BseMI	GCAATG	2 cut(s)	1245, 3173
BseMII	CTCAG	8 cut(s)	1467, 1842, 2312, 2439, 2565, 2684, 3132, 3615
BseNI	ACTGG	6 cut(s)	265, 997, 1573, 2414, 2501, 3420
BseRI	GAGGAG	2 cut(s)	1817, 3350
BseSI	GKGCMC	2 cut(s)	738, 1186
BseX3I	CGGCCG	1 cut(s)	151
BsgI	GTGCAG	4 cut(s)	241, 326, 1377, 1910
Bsh1236I	CGCG	1 cut(s)	156
Bsh1285I	CGRYCG	3 cut(s)	121, 154, 2644
BshFI	GGCC	7 cut(s)	153, 299, 628, 1162, 1878, 1974, 2070
BshNI	GGYRCC	4 cut(s)	113, 1183, 1193, 2410
BshVI	ATCGAT	1 cut(s)	121
BsiEI	CGRYCG	3 cut(s)	121, 154, 2644
BsiHKAI	GWGCWC	4 cut(s)	937, 1175, 1255, 1351
BsiHKCI	CYCGRG	3 cut(s)	561, 1002, 1620
BsiSI	CCGG	6 cut(s)	194, 1149, 1163, 1275, 1353, 2948
BslFI	GGGAC	2 cut(s)	476, 2172
BslI	CCNNNNNNNGG	7 cut(s)	713, 901, 1082, 1280, 1970, 2267, 3515
BsmAI	GTCTC	5 cut(s)	977, 1623, 2673, 3359, 3754
BsmBI	CGTCTC	1 cut(s)	3359
BsmFI	GGGAC	2 cut(s)	476, 2172
BsnI	GGCC	7 cut(s)	153, 299, 628, 1162, 1878, 1974, 2070
BsoBI	CYCGRG	3 cut(s)	561, 1002, 1620
Bsp119I	TTCGAA	1 cut(s)	3476
Bsp1286I	GDGCHC	9 cut(s)	562, 738, 909, 937, 1175, 1186, 1255, 1351, 1849
Bsp19I	CCATGG	1 cut(s)	859
BspACI	CCGC	9 cut(s)	21, 24, 27, 154, 156, 433, 2507, 2861, 2936
BspANI	GGCC	7 cut(s)	153, 299, 628, 1162, 1878, 1974, 2070
BspCNI	CTCAG	8 cut(s)	1468, 1843, 2311, 2440, 2564, 2683, 3131, 3614
BspDI	ATCGAT	1 cut(s)	121
BspFNI	CGCG	1 cut(s)	156
BspHI	TCATGA	4 cut(s)	508, 1404, 1762, 3235
BspMI	ACCTGC	2 cut(s)	579, 2470
BspOI	GCTAGC	1 cut(s)	3299
BspPI	GGATC	3 cut(s)	2875, 2927, 3317
BspQI	GCTCTTC	1 cut(s)	2740
BspT104I	TTCGAA	1 cut(s)	3476
BspT107I	GGYRCC	4 cut(s)	113, 1183, 1193, 2410
BspTI	CTTAAG	3 cut(s)	1699, 3146, 3644
BsrDI	GCAATG	2 cut(s)	1245, 3173
BsrFI	RCCGGY	2 cut(s)	1148, 1162
BsrI	ACTGG	6 cut(s)	265, 997, 1573, 2414, 2501, 3420
BssAI	RCCGGY	2 cut(s)	1148, 1162
BssECI	CCNNGG	5 cut(s)	154, 859, 1273, 1306, 1964
BssNI	GRCGYC	2 cut(s)	114, 159
BssSI	CACGAG	2 cut(s)	1686, 3372
BssT1I	CCWWGG	2 cut(s)	859, 1964
Bst2BI	CACGAG	2 cut(s)	1686, 3372
Bst2UI	CCWGG	5 cut(s)	321, 930, 1188, 2067, 3510
Bst4CI	ACNGT	6 cut(s)	38, 311, 1127, 1177, 1741, 2017
Bst6I	CTCTTC	8 cut(s)	358, 434, 2236, 2529, 2740, 2841, 3048, 3720
BstACI	GRCGYC	2 cut(s)	114, 159
BstAFI	CTTAAG	3 cut(s)	1699, 3146, 3644
BstAPI	GCANNNNNTGC	3 cut(s)	1244, 1364, 2465
BstBAI	YACGTR	3 cut(s)	385, 1380, 3611
BstBI	TTCGAA	1 cut(s)	3476
BstC8I	GCNNGC	8 cut(s)	603, 967, 1150, 1164, 1717, 2238, 3180, 3297
BstDSI	CCRYGG	2 cut(s)	154, 859
BstFNI	CGCG	1 cut(s)	156
BstH2I	RGCGCY	3 cut(s)	117, 1090, 2573
BstHHI	GCGC	4 cut(s)	116, 1089, 2572, 3780
BstMAI	GTCTC	5 cut(s)	977, 1623, 2673, 3359, 3754
BstMCI	CGRYCG	3 cut(s)	121, 154, 2644
BstNI	CCWGG	5 cut(s)	321, 930, 1188, 2067, 3510
BstNSI	RCATGY	4 cut(s)	339, 872, 2794, 3776
BstSCI	CCNGG	9 cut(s)	192, 319, 928, 1186, 1273, 1351, 2065, 2946, 3508
BstSFI	CTRYAG	2 cut(s)	1536, 2292
BstSLI	GKGCMC	2 cut(s)	738, 1186
BstUI	CGCG	1 cut(s)	156
BstV2I	GAAGAC	2 cut(s)	3003, 3546
BstX2I	RGATCY	3 cut(s)	1327, 2867, 2932
BstXI	CCANNNNNNTGG	1 cut(s)	1638
BstYI	RGATCY	3 cut(s)	1327, 2867, 2932
BstZI	CGGCCG	1 cut(s)	151
Bsu15I	ATCGAT	1 cut(s)	121
BsuRI	GGCC	7 cut(s)	153, 299, 628, 1162, 1878, 1974, 2070
BsuTUI	ATCGAT	1 cut(s)	121
BtgI	CCRYGG	2 cut(s)	154, 859
BtrI	CACGTC	2 cut(s)	346, 3574
BtsIMutI	CAGTG	6 cut(s)	258, 1173, 1746, 1824, 2013, 3413
BveI	ACCTGC	2 cut(s)	579, 2470
Cac8I	GCNNGC	8 cut(s)	603, 967, 1150, 1164, 1717, 2238, 3180, 3297
CaiI	CAGNNNCTG	2 cut(s)	992, 3515
CciI	TCATGA	4 cut(s)	508, 1404, 1762, 3235
CfoI	GCGC	4 cut(s)	116, 1089, 2572, 3780
Cfr10I	RCCGGY	2 cut(s)	1148, 1162
Cfr13I	GGNCC	8 cut(s)	190, 568, 626, 1160, 1310, 1383, 1973, 3506
Cfr42I	CCGCGG	1 cut(s)	157
ClaI	ATCGAT	1 cut(s)	121
CseI	GACGC	1 cut(s)	148
CsiI	ACCWGGT	1 cut(s)	1186
Csp6I	GTAC	6 cut(s)	795, 1194, 1628, 2096, 3397, 3700
CspCI	CAANNNNNGTGG	2 cut(s)	2322, 2357
CviQI	GTAC	6 cut(s)	795, 1194, 1628, 2096, 3397, 3700
DinI	GGCGCC	1 cut(s)	115
DraI	TTTAAA	1 cut(s)	3550
DraIII	CACNNNGTG	2 cut(s)	1190, 3748
EaeI	YGGCCR	2 cut(s)	151, 1876
EagI	CGGCCG	1 cut(s)	151
Eam1104I	CTCTTC	8 cut(s)	358, 434, 2236, 2529, 2740, 2841, 3048, 3720
EarI	CTCTTC	8 cut(s)	358, 434, 2236, 2529, 2740, 2841, 3048, 3720
EclXI	CGGCCG	1 cut(s)	151
Eco130I	CCWWGG	2 cut(s)	859, 1964
Eco24I	GRGCYC	3 cut(s)	562, 909, 1849
Eco47I	GGWCC	5 cut(s)	190, 568, 1310, 1383, 3506
Eco52I	CGGCCG	1 cut(s)	151
Eco57I	CTGAAG	4 cut(s)	312, 2451, 2631, 3489
Eco72I	CACGTG	2 cut(s)	385, 3611
Eco88I	CYCGRG	3 cut(s)	561, 1002, 1620
EcoO109I	RGGNCCY	2 cut(s)	568, 626
EcoRI	GAATTC	1 cut(s)	2487
EcoRII	CCWGG	5 cut(s)	319, 928, 1186, 2065, 3508
EcoT14I	CCWWGG	2 cut(s)	859, 1964
EcoT22I	ATGCAT	1 cut(s)	3774
EcoT38I	GRGCYC	3 cut(s)	562, 909, 1849
EgeI	GGCGCC	1 cut(s)	115
EheI	GGCGCC	1 cut(s)	115
ErhI	CCWWGG	2 cut(s)	859, 1964
Esp3I	CGTCTC	1 cut(s)	3359
FalI	AAGNNNNNCTT	4 cut(s)	2085, 2117, 3186, 3218
FaqI	GGGAC	2 cut(s)	476, 2172
FauI	CCCGC	1 cut(s)	440
FauNDI	CATATG	3 cut(s)	1225, 2908, 3754
FbaI	TGATCA	2 cut(s)	1041, 3376
FblI	GTMKAC	2 cut(s)	2026, 3570
FriOI	GRGCYC	3 cut(s)	562, 909, 1849
FspBI	CTAG	7 cut(s)	242, 1302, 1710, 2390, 2441, 3296, 3410
GlaI	GCGC	4 cut(s)	115, 1088, 2571, 3779
GsuI	CTGGAG	3 cut(s)	204, 342, 951
HaeII	RGCGCY	3 cut(s)	117, 1090, 2573
HaeIII	GGCC	7 cut(s)	153, 299, 628, 1162, 1878, 1974, 2070
HapII	CCGG	6 cut(s)	194, 1149, 1163, 1275, 1353, 2948
HgaI	GACGC	1 cut(s)	148
HhaI	GCGC	4 cut(s)	116, 1089, 2572, 3780
Hin1I	GRCGYC	2 cut(s)	114, 159
Hin6I	GCGC	4 cut(s)	114, 1087, 2570, 3778
HinP1I	GCGC	4 cut(s)	114, 1087, 2570, 3778
HincII	GTYRAC	6 cut(s)	349, 1096, 1591, 2027, 2170, 3571
HindII	GTYRAC	6 cut(s)	349, 1096, 1591, 2027, 2170, 3571
HindIII	AAGCTT	2 cut(s)	2248, 2721
HpaII	CCGG	6 cut(s)	194, 1149, 1163, 1275, 1353, 2948
HphI	GGTGA	5 cut(s)	304, 386, 1289, 2729, 3760
Hpy99I	CGWCG	6 cut(s)	38, 131, 152, 164, 1398, 3575
HpyCH4III	ACNGT	6 cut(s)	38, 311, 1127, 1177, 1741, 2017
Hsp92I	GRCGYC	2 cut(s)	114, 159
HspAI	GCGC	4 cut(s)	114, 1087, 2570, 3778
KasI	GGCGCC	1 cut(s)	113
KpnI	GGTACC	1 cut(s)	1197
KroI	GCCGGC	2 cut(s)	1148, 1162
KroNI	GCCGGC	2 cut(s)	1150, 1164
Ksp22I	TGATCA	2 cut(s)	1041, 3376
KspI	CCGCGG	1 cut(s)	157
LguI	GCTCTTC	1 cut(s)	2740
MabI	ACCWGGT	1 cut(s)	1186
MaeI	CTAG	7 cut(s)	242, 1302, 1710, 2390, 2441, 3296, 3410
MaeIII	GTNAC	9 cut(s)	214, 841, 1068, 1815, 2221, 2537, 3463, 3604, 3748
MflI	RGATCY	3 cut(s)	1327, 2867, 2932
MhlI	GDGCHC	9 cut(s)	562, 738, 909, 937, 1175, 1186, 1255, 1351, 1849
Mly113I	GGCGCC	1 cut(s)	114
MlyI	GAGTC	6 cut(s)	80, 685, 994, 2165, 2675, 3364
MmeI	TCCRAC	2 cut(s)	1414, 2552
Mph1103I	ATGCAT	1 cut(s)	3774
MroNI	GCCGGC	2 cut(s)	1148, 1162
MroXI	GAANNNNTTC	3 cut(s)	1791, 3010, 3057
MslI	CAYNNNNRTG	5 cut(s)	507, 533, 1032, 1767, 1932
MspA1I	CMGCKG	2 cut(s)	156, 2818
MspCI	CTTAAG	3 cut(s)	1699, 3146, 3644
MspI	CCGG	6 cut(s)	194, 1149, 1163, 1275, 1353, 2948
MspR9I	CCNGG	9 cut(s)	194, 321, 930, 1188, 1275, 1353, 2067, 2948, 3510
MvaI	CCWGG	5 cut(s)	321, 930, 1188, 2067, 3510
MvnI	CGCG	1 cut(s)	156
NaeI	GCCGGC	2 cut(s)	1150, 1164
NarI	GGCGCC	1 cut(s)	114
NciI	CCSGG	4 cut(s)	194, 1275, 1353, 2948
NcoI	CCATGG	1 cut(s)	859
NdeI	CATATG	3 cut(s)	1225, 2908, 3754
NgoMIV	GCCGGC	2 cut(s)	1148, 1162
NheI	GCTAGC	1 cut(s)	3295
NmeAIII	GCCGAG	1 cut(s)	1331
NmuCI	GTSAC	2 cut(s)	2537, 3748
NsiI	ATGCAT	1 cut(s)	3774
NspI	RCATGY	4 cut(s)	339, 872, 2794, 3776
NspV	TTCGAA	1 cut(s)	3476
PaeR7I	CTCGAG	1 cut(s)	1002
PagI	TCATGA	4 cut(s)	508, 1404, 1762, 3235
PaqCI	CACCTGC	1 cut(s)	2470
PciI	ACATGT	1 cut(s)	868
PciSI	GCTCTTC	1 cut(s)	2740
PcsI	WCGNNNNNNNCGW	3 cut(s)	95, 156, 3683
PdiI	GCCGGC	2 cut(s)	1150, 1164
PdmI	GAANNNNTTC	3 cut(s)	1791, 3010, 3057
PfeI	GAWTC	5 cut(s)	123, 179, 1986, 3500, 3594
PflMI	CCANNNNNTGG	2 cut(s)	1082, 3515
Ple19I	CGATCG	2 cut(s)	121, 2644
PleI	GAGTC	6 cut(s)	79, 685, 994, 2165, 2674, 3364
PluTI	GGCGCC	1 cut(s)	117
PmaCI	CACGTG	2 cut(s)	385, 3611
PmlI	CACGTG	2 cut(s)	385, 3611
PpsI	GAGTC	6 cut(s)	79, 685, 994, 2165, 2674, 3364
Ppu21I	YACGTR	3 cut(s)	385, 1380, 3611
PpuMI	RGGWCCY	1 cut(s)	568
PscI	ACATGT	1 cut(s)	868
PshBI	ATTAAT	1 cut(s)	2370
PsiI	TTATAA	1 cut(s)	393
Psp1406I	AACGTT	1 cut(s)	3043
Psp5II	RGGWCCY	1 cut(s)	568
Psp6I	CCWGG	5 cut(s)	319, 928, 1186, 2065, 3508
PspCI	CACGTG	2 cut(s)	385, 3611
PspGI	CCWGG	5 cut(s)	319, 928, 1186, 2065, 3508
PspPI	GGNCC	8 cut(s)	190, 568, 626, 1160, 1310, 1383, 1973, 3506
PspPPI	RGGWCCY	1 cut(s)	568
PspXI	VCTCGAGB	1 cut(s)	1002
PsrI	GAACNNNNNNTAC	2 cut(s)	1053, 1085
PstNI	CAGNNNCTG	2 cut(s)	992, 3515
PsuI	RGATCY	3 cut(s)	1327, 2867, 2932
PvuI	CGATCG	2 cut(s)	121, 2644
PvuII	CAGCTG	1 cut(s)	2818
RsaI	GTAC	6 cut(s)	796, 1195, 1629, 2097, 3398, 3701
RsaNI	GTAC	6 cut(s)	795, 1194, 1628, 2096, 3397, 3700
RseI	CAYNNNNRTG	5 cut(s)	507, 533, 1032, 1767, 1932
SacII	CCGCGG	1 cut(s)	157
SalI	GTCGAC	2 cut(s)	2025, 3569
SapI	GCTCTTC	1 cut(s)	2740
Sau96I	GGNCC	8 cut(s)	190, 568, 626, 1160, 1310, 1383, 1973, 3506
SchI	GAGTC	6 cut(s)	80, 685, 994, 2165, 2675, 3364
ScrFI	CCNGG	9 cut(s)	194, 321, 930, 1188, 1275, 1353, 2067, 2948, 3510
SduI	GDGCHC	9 cut(s)	562, 738, 909, 937, 1175, 1186, 1255, 1351, 1849
SexAI	ACCWGGT	1 cut(s)	1186
SfcI	CTRYAG	2 cut(s)	1536, 2292
SfoI	GGCGCC	1 cut(s)	115
Sfr274I	CTCGAG	1 cut(s)	1002
Sfr303I	CCGCGG	1 cut(s)	157
SfuI	TTCGAA	1 cut(s)	3476
SgrBI	CCGCGG	1 cut(s)	157
SinI	GGWCC	5 cut(s)	190, 568, 1310, 1383, 3506
SlaI	CTCGAG	1 cut(s)	1002
SmiMI	CAYNNNNRTG	5 cut(s)	507, 533, 1032, 1767, 1932
SmlI	CTYRAG	6 cut(s)	695, 1002, 1699, 2622, 3146, 3644
SmoI	CTYRAG	6 cut(s)	695, 1002, 1699, 2622, 3146, 3644
SpeI	ACTAGT	1 cut(s)	2389
SsiI	CCGC	9 cut(s)	21, 24, 27, 154, 156, 433, 2507, 2861, 2936
SspDI	GGCGCC	1 cut(s)	113
SspMI	CTAG	7 cut(s)	242, 1302, 1710, 2390, 2441, 3296, 3410
StyD4I	CCNGG	9 cut(s)	192, 319, 928, 1186, 1273, 1351, 2065, 2946, 3508
StyI	CCWWGG	2 cut(s)	859, 1964
TaaI	ACNGT	6 cut(s)	38, 311, 1127, 1177, 1741, 2017
TauI	GCSGC	5 cut(s)	24, 27, 156, 159, 2510
TfiI	GAWTC	5 cut(s)	123, 179, 1986, 3500, 3594
TscAI	CASTG	6 cut(s)	265, 1180, 1746, 1831, 2020, 3420
TseFI	GTSAC	2 cut(s)	2537, 3748
Tsp45I	GTSAC	2 cut(s)	2537, 3748
TspGWI	ACGGA	3 cut(s)	1411, 3606, 3692
TspRI	CASTG	6 cut(s)	265, 1180, 1746, 1831, 2020, 3420
Van91I	CCANNNNNTGG	2 cut(s)	1082, 3515
Vha464I	CTTAAG	3 cut(s)	1699, 3146, 3644
VpaK11BI	GGWCC	5 cut(s)	190, 568, 1310, 1383, 3506
VspI	ATTAAT	1 cut(s)	2370
XapI	RAATTY	6 cut(s)	237, 512, 1757, 2487, 2660, 3302
XbaI	TCTAGA	1 cut(s)	241
XceI	RCATGY	4 cut(s)	339, 872, 2794, 3776
XhoI	CTCGAG	1 cut(s)	1002
XmiI	GTMKAC	2 cut(s)	2026, 3570
XmnI	GAANNNNTTC	3 cut(s)	1791, 3010, 3057
XspI	CTAG	7 cut(s)	242, 1302, 1710, 2390, 2441, 3296, 3410
Zsp2I	ATGCAT	1 cut(s)	3774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.