AT4G21820

Calponin homology (CH) domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006240
Physical Location & Seq
Forward (+)
11577419 .. 11584073
6655 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21820.3

Sequence Viewer

Length: 3768 bp
ATGGATGAAAACGAACCGCCGTGCGCTTCACCGGCGCCGCCACGTAATCCGGCGTCATCGTTACTCAGTGACATATCCAATTTCAAAACGCCGCGGCGTACTTCCGTTGTGAATTCGAATATTAGTAAATCTCCGTATCCGCATTTCTTCACTGCATCAAAGCAAACCCCAAAATCTTCGTCTTCTAATTTCCGTCGTCCTTCAATGGTTCATTCTTATGCATCTCGGTCTAAGGTATCAACATCGTCGAGGCGGCTCAAAGCATTTGAACTTCAGCAATCTCAATCTTCACGCAAGGCTGAGTTGACGAAAGAGAAGAATCTTAGATCTCTTGCTAAATCACTTACTGTGTGGCTCAATTTCTTGTTTGAAAACCCGGAAAATTGCGGTTGCGATCCGTTCGAGAACGAGTCTGGTGTTGGGAATTTAGGTAAGGGGAAGAGAGATAGTGGTGAGGCTTTAGGAAATAGTAAATCTGTTGGAGTTGATACAATGTGGAGAAGCCCTAAAAGGTTGAGGAATTTAGGTTGGTGTGGTGAGAAAAAACGTTCAGAGATTGACTCTTCCTTGACTGGTTCTAAGTATTCTACGTTGAGAGAATCTTTAAGAGATGTGTGTAGTTTAGATGATTTGAAGCAAAGAATGCAATTTCACTTGAGTTTGGGAAGTTGCAAGGAGATTTTTGATGTGATGACTCGTGTTTCAAAGAATATAGATGAAGGGAGGATAAAAATGAAACCACAATGCCCTCTTGTAACTGATTTTGGAATGAAGGAGAAGGCCATCAAGGCACTGATGAGCTATAACCAAGTTTGGCTTCGTTTAGGGTTATATATCATCTTTGGTGGTGATTCCTTTTTGTCTGATAGTGAAGTTAACTCAGATCAAGAAATGGCGTTCTTGAAGATGGTAATTAGTAAGCAGTTCTTTTCCCACGATGGCCTTGCTAGATCTTATGTTTATAACAAGATGGTTGAGGGTTTATACAGACCAGGGTACTATGAAGCTCTTGGAAATGTGATTTTGAAGAGGATTCTGTTGCTGGTTCTTGTAATTGATAGAGCCAAATCTCAAAGCTGCCTTTCGCTCAAGTATGGGATTGATGGGATAGATGGTGGCTCACCCCTCATGTTCTCAGAGAAATCTAGTATAAAGTCTAGCCACCAACTTATATGTGAATTATTGTCCTCTGATGTAATGCATGGAGAAGGGAATCTCCTAGCGCATCTAGTGATTATAGGATACAAAATACCTTATCAGCAGTCTCCTTTAGTTGAATACAACTTTAGAGTGAGAGAGTTGTTTGCTGACCTCCAAGATGGCGTGCGGCTCTGCAGAGCTATTCAACTACTACTTCATGATCCGTCCATTCTCACGAAAATGGTAGTTCCATCCGACAATCGAAAGAAGAACTTGGCCAATTGTAGAATTGCACTTCAATATCTTAAGGATGCTGGTGTTTCGTTAAAAGATGACGAAGGAATGATGATAACTGGGGAAGATGTTGCAGATGGTGACAGAGAGCTCACTATTTCACTACTATGGAACATTTTTGTACATTTGCAGCTGCCTCTTCTGATTAATGGGAGACTCTTGACAGAGGAAATCTACAAAGTCCAGGGAGTGGAACAGAATAATCAAATTACCATGTCTACTCCTCTGGAAATGCTCTTGAACTGGATCCAGGATCCTGGTGGTCAAGAAGGCCCTCAATCAGTAATGTCCAATACCGATTACCATGATGCAGTTCAAAATTTTATTCTGTCGCAAAAGTTGACAGCACTTTTGGGAAGCTTTCCTGAGATTGGTGACTTACTGGAACATAATGCAGTAGTTAGCAACCAAAGTGTGATTATACTGTTGGCTTTCCTATCATCAAAGCTGATCGTCAAGGAGAATTTGTTAGATTTTACATTGGCAGGTTATCTTACAAATTCCGTCTTTTCTCAGGAGCAACTGAATTTTCATAAGTTGCTGTGCTCTAGCTGTCAAGTTCAGGAGAAGAGATATTCGCGCATCAGAATCAGTTGCAGCAGCTCTGAAGCAGTTACAATCGAGGAACCAGACAGAGAAAATGGAGAAGATGCTACTAAAAGGTTCCAGGCAATCAAAGCCTGGTGGCAGGACATGGCCAACCAAAATCAAATTTCTGTTGGAAAAGCTAATAGCCATACTCTGCAAGGCTCCTTGTCTAGAAAATGCACCACGGATTCCCAACGAGCATACCTCTTACTTGCAGAAATTGCAGCAGTGATCATACAATCGAATATTAGGGGACTTCATGCACGCCGCAAGTTTAGAAATAAGATGAGAGCAATCTGCTTCTTACAAGCTGCTGTTCGGACATGGTTATCAGTGAAAAATATACAAGTTGTTGAGAAATTCAATGTTGAGGAAGTTACTTTACATTTATCTGAAAGATCGGCCAACTTAAAACCTGTAGCTAGATATGTCAAGTTCATTGTTGATCGAAGTCGTTTCATCAAGTTGAGGAAATCTGTTTCCGTCATTCAGAAAGCTGTAAGGAGACATCAGAGCAACCTTCATCATGAGCTGAAAGCAGCACTCAGAATTCAACTAGCTTGGAGGAGTTATAAAGAAAAAGTTATCTCTTCTATCACCATCCAATCTTATGTTCGTGGATGGATCACACGCAGAATGAATCGGACATACAAGTTTTCTTCCATACTTATTCAAAGATACTGCCGTGGTTGGTTGGCGAGAAGGAAGTTTTGTCTTCAGAGAGAAGCAACGATAAGCATTCAGAGTGCTATCCGAAAATTTAACTGCATGATGTCATTTCATCGTTGCAAGCATGCAGCCACACAGGTTCAACGGCTTGTTAGGGGACAAATTGTTCGAAGCAGGCTTCAAGGAGCTTCTACTCTCTATTCAAAACTTGACGAAGGCGTTTCAAGACTTCCACAACACAGCTTTGGGATGACAAAAATGCTACATTCCGTCATTAAAGTGCAGCGTTGGTGGAGAGAATTTCTCCATTCAAAGAATATGAGAATAAAATCAGCAGTCTTAATACAGAGTCATATTCGAGGTTTATTTGCCAGACGAAAAACTTCTGTGGAAAGACATAACATTGTCATGATTCAATCACACTGGAGAGGTTATCTCACACGCAAAGCATCAAAGGCTCAAGTTCTGGATCTGAGAGTACGAATGCAAACTTCTGCAGCAAACATAGATGACAAGAAACGGTTGATAAACAAGCTTCTTTCTGCGCTTTCGGAACTACTGAGCATGAAAAACGTCCACAACATTCTTCACATTTGTGAAACTTTGGAATCGGCAACCAAATACTCTGACAAATGCTGTGAAGAGCTTGTGGCAGCTGGAGCTATAGAAAAGTTGCTAACATTGATCCGGTCTGCAAGCAGAAGCATCCCTGATCAACAAGTTTCAAAACATGCACTATCAACTTTGGGACATCTTGCTCGTTATCCACAAATGGCAGATGAGCTAATCAACACGAAAGGTTCCATTCAGACAATCTTCTGGGAACTACTCAGGAACAAAGAAGAAGCATATTTCATTGCATCAGATGTTCTGAAGAAAATATGCAGTAGCCATAAAGGCGTAGAAGCAGTTCGAAAGCTTCCTGCACTGGTAAAACGGTTACACGTCTTAGTTGAGGAGCTAACTCGTAAGGCAAACATCGAGAAACGGAACGTTAAGGGACAGACTGGAAAAGAAAAGAGTGAGAGAAGATTAAAAGAAGCTATTGAGCTTGTTAAGCTTATAACCAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm CH IQ

Protein Analysis

1255

Amino Acids

142.81

Weight (kDa)

9.79

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 428 - 520 5.8e-07 Calponin homology (CH) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 963, 2595, 3755
Acc36I ACCTGC 1 cut(s) 1910
AccB1I GGYRCC 1 cut(s) 34
AccB7I CCANNNNNTGG 1 cut(s) 1624
AccI GTMKAC 1 cut(s) 1652
AccII CGCG 2 cut(s) 94, 2014
AciI CCGC 9 cut(s) 17, 38, 92, 94, 140, 253, 387, 1327, 2290
AclI AACGTT 2 cut(s) 547, 3684
AclWI GGATC 9 cut(s) 389, 1355, 1675, 1682, 1688, 1695, 2654, 3168, 3370
AcoI YGGCCR 3 cut(s) 1416, 2130, 2424
AcuI CTGAAG 4 cut(s) 257, 2061, 2723, 3584
AcyI GRCGYC 2 cut(s) 35, 53
AfaI GTAC 4 cut(s) 100, 998, 1557, 3171
AfiI CCNNNNNNNGG 2 cut(s) 1624, 1805
AflII CTTAAG 1 cut(s) 1445
AflIII ACRYGT 1 cut(s) 3634
AjiI CACGTC 1 cut(s) 3637
AjnI CCWGG 6 cut(s) 991, 1617, 1683, 1690, 2100, 2114
AjuI GAANNNNNNNTTGG 4 cut(s) 801, 833, 2619, 2651
AleI CACNNNNGTG 1 cut(s) 3285
AloI GAACNNNNNNTCC 2 cut(s) 2841, 2873
Alw21I GWGCWC 2 cut(s) 1527, 1982
Alw26I GTCTC 3 cut(s) 1269, 1582, 2521
AlwI GGATC 9 cut(s) 389, 1355, 1675, 1682, 1688, 1695, 2654, 3168, 3370
AoxI GGCC 6 cut(s) 780, 940, 1416, 1705, 2130, 2424
ArsI GACNNNNNNTTYG 6 cut(s) 164, 196, 1768, 1800, 2841, 2873
AseI ATTAAT 1 cut(s) 1581
Asp700I GAANNNNTTC 2 cut(s) 3073, 3600
AspLEI GCGC 5 cut(s) 26, 37, 1225, 2016, 3238
AspS9I GGNCC 1 cut(s) 1706
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 8 cut(s) 21, 464, 548, 860, 1113, 1526, 1820, 2611
AsuII TTCGAA 3 cut(s) 116, 2860, 3604
BaeI ACNNNNGTAYC 2 cut(s) 2692, 2725
BalI TGGCCA 2 cut(s) 1418, 2132
BamHI GGATCC 2 cut(s) 1680, 1687
BanI GGYRCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 1527
BarI GAAGNNNNNNTAC 2 cut(s) 2388, 2420
BauI CACGAG 1 cut(s) 696
BbsI GAAGAC 2 cut(s) 174, 2729
Bbv12I GWGCWC 2 cut(s) 1527, 1982
BceAI ACGGC 3 cut(s) 4, 2691, 2852
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BciT130I CCWGG 6 cut(s) 993, 1619, 1685, 1692, 2102, 2116
BciVI GTATCC 2 cut(s) 147, 1235
BclI TGATCA 2 cut(s) 2253, 3403
BcnI CCSGG 1 cut(s) 377
BcoDI GTCTC 3 cut(s) 1269, 1582, 2521
BfaI CTAG 9 cut(s) 948, 1146, 1158, 1220, 1229, 1983, 2193, 2445, 2579
BfmI CTRYAG 4 cut(s) 1333, 2439, 3186, 3354
BfoI RGCGCY 1 cut(s) 38
BfrI CTTAAG 1 cut(s) 1445
BfuAI ACCTGC 1 cut(s) 1910
BfuI GTATCC 2 cut(s) 147, 1235
BglI GCCNNNNNGGC 2 cut(s) 788, 3588
BglII AGATCT 2 cut(s) 326, 950
Bme1390I CCNGG 7 cut(s) 377, 993, 1619, 1685, 1692, 2102, 2116
BmgBI CACGTC 1 cut(s) 3637
BmgT120I GGNCC 1 cut(s) 1706
BmiI GGNNCC 7 cut(s) 36, 1682, 1689, 2061, 2099, 2185, 3493
BmrFI CCNGG 7 cut(s) 377, 993, 1619, 1685, 1692, 2102, 2116
BmrI ACTGGG 1 cut(s) 1503
BmuI ACTGGG 1 cut(s) 1503
BpiI GAAGAC 2 cut(s) 174, 2729
BplI GAGNNNNNCTC 8 cut(s) 545, 577, 2211, 2243, 2979, 3011, 3111, 3143
BpmI CTGGAG 2 cut(s) 3136, 3369
Bpu14I TTCGAA 3 cut(s) 116, 2860, 3604
BpuEI CTTGAG 3 cut(s) 676, 1073, 3135
BpuMI CCSGG 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 44
BsaHI GRCGYC 2 cut(s) 35, 53
BsaJI CCNNGG 5 cut(s) 92, 992, 1618, 2205, 2707
BsaWI WCCGGW 1 cut(s) 3378
BsaXI ACNNNNNCTCC 2 cut(s) 1886, 1916
Bsc4I CCNNNNNNNGG 2 cut(s) 1624, 1805
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 7 cut(s) 577, 1498, 1682, 1821, 3119, 3624, 3703
Bse3DI GCAATG 1 cut(s) 3546
BseBI CCWGG 6 cut(s) 993, 1619, 1685, 1692, 2102, 2116
BseDI CCNNGG 5 cut(s) 92, 992, 1618, 2205, 2707
BseGI GGATG 7 cut(s) 10, 1391, 1456, 2622, 2648, 2946, 3396
BseLI CCNNNNNNNGG 2 cut(s) 1624, 1805
BseMI GCAATG 1 cut(s) 3546
BseNI ACTGG 7 cut(s) 577, 1498, 1682, 1821, 3119, 3624, 3703
BseRI GAGGAG 3 cut(s) 1647, 2602, 3662
BsgI GTGCAG 2 cut(s) 2993, 3600
Bsh1236I CGCG 2 cut(s) 94, 2014
BshFI GGCC 6 cut(s) 782, 942, 1418, 1707, 2132, 2426
BshNI GGYRCC 1 cut(s) 34
BsiHKAI GWGCWC 2 cut(s) 1527, 1982
BsiSI CCGG 4 cut(s) 32, 50, 377, 3379
BslFI GGGAC 4 cut(s) 2289, 2862, 3453, 3705
BslI CCNNNNNNNGG 2 cut(s) 1624, 1805
BsmAI GTCTC 3 cut(s) 1269, 1582, 2521
BsmFI GGGAC 4 cut(s) 2289, 2862, 3453, 3705
BsmI GAATGC 3 cut(s) 648, 2760, 3180
BsnI GGCC 6 cut(s) 782, 942, 1418, 1707, 2132, 2426
Bsp119I TTCGAA 3 cut(s) 116, 2860, 3604
Bsp1286I GDGCHC 2 cut(s) 1527, 1982
Bsp1407I TGTACA 1 cut(s) 1555
BspACI CCGC 9 cut(s) 17, 38, 92, 94, 140, 253, 387, 1327, 2290
BspANI GGCC 6 cut(s) 782, 942, 1418, 1707, 2132, 2426
BspFNI CGCG 2 cut(s) 94, 2014
BspHI TCATGA 3 cut(s) 1357, 2548, 3099
BspLI GGNNCC 7 cut(s) 36, 1682, 1689, 2061, 2099, 2185, 3493
BspMAI CTGCAG 2 cut(s) 1337, 3190
BspMI ACCTGC 1 cut(s) 1910
BspPI GGATC 9 cut(s) 389, 1355, 1675, 1682, 1688, 1695, 2654, 3168, 3370
BspQI GCTCTTC 1 cut(s) 3327
BspT104I TTCGAA 3 cut(s) 116, 2860, 3604
BspT107I GGYRCC 1 cut(s) 34
BspTI CTTAAG 1 cut(s) 1445
BsrDI GCAATG 1 cut(s) 3546
BsrFI RCCGGY 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 1555
BsrI ACTGG 7 cut(s) 577, 1498, 1682, 1821, 3119, 3624, 3703
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 5 cut(s) 92, 992, 1618, 2205, 2707
BssNI GRCGYC 2 cut(s) 35, 53
BssSI CACGAG 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 6 cut(s) 993, 1619, 1685, 1692, 2102, 2116
Bst4CI ACNGT 4 cut(s) 349, 1860, 3213, 3630
Bst6I CTCTTC 7 cut(s) 434, 568, 1022, 1578, 1997, 2617, 3327
BstACI GRCGYC 2 cut(s) 35, 53
BstAFI CTTAAG 1 cut(s) 1445
BstAPI GCANNNNNTGC 2 cut(s) 643, 2243
BstAUI TGTACA 1 cut(s) 1555
BstBAI YACGTR 1 cut(s) 44
BstBI TTCGAA 3 cut(s) 116, 2860, 3604
BstC8I GCNNGC 6 cut(s) 1325, 2287, 2813, 2817, 2867, 3388
BstDSI CCRYGG 3 cut(s) 92, 2205, 2707
BstF5I GGATG 7 cut(s) 10, 1391, 1456, 2622, 2648, 2946, 3396
BstFNI CGCG 2 cut(s) 94, 2014
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 5 cut(s) 26, 37, 1225, 2016, 3238
BstMAI GTCTC 3 cut(s) 1269, 1582, 2521
BstMWI GCNNNNNNNGC 9 cut(s) 32, 643, 788, 2111, 2243, 3146, 3350, 3588, 3748
BstNI CCWGG 6 cut(s) 993, 1619, 1685, 1692, 2102, 2116
BstNSI RCATGY 2 cut(s) 2819, 3425
BstSCI CCNGG 7 cut(s) 375, 991, 1617, 1683, 1690, 2100, 2114
BstSFI CTRYAG 4 cut(s) 1333, 2439, 3186, 3354
BstUI CGCG 2 cut(s) 94, 2014
BstV2I GAAGAC 2 cut(s) 174, 2729
BstX2I RGATCY 5 cut(s) 326, 950, 1680, 1687, 3160
BstXI CCANNNNNNTGG 1 cut(s) 1691
BstYI RGATCY 5 cut(s) 326, 950, 1680, 1687, 3160
BsuI GTATCC 2 cut(s) 147, 1235
BsuRI GGCC 6 cut(s) 782, 942, 1418, 1707, 2132, 2426
BtgI CCRYGG 3 cut(s) 92, 2205, 2707
BtrI CACGTC 1 cut(s) 3637
BtsCI GGATG 7 cut(s) 10, 1391, 1456, 2622, 2648, 2946, 3396
BtsI GCAGTG 2 cut(s) 150, 2256
BtsIMutI CAGTG 7 cut(s) 73, 150, 791, 2256, 2361, 3112, 3617
BveI ACCTGC 1 cut(s) 1910
Cac8I GCNNGC 6 cut(s) 1325, 2287, 2813, 2817, 2867, 3388
CciI TCATGA 3 cut(s) 1357, 2548, 3099
CfoI GCGC 5 cut(s) 26, 37, 1225, 2016, 3238
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 1706
Cfr42I CCGCGG 1 cut(s) 95
CseI GACGC 1 cut(s) 42
Csp6I GTAC 4 cut(s) 99, 997, 1556, 3170
CviQI GTAC 4 cut(s) 99, 997, 1556, 3170
DinI GGCGCC 1 cut(s) 36
EaeI YGGCCR 3 cut(s) 1416, 2130, 2424
Eam1104I CTCTTC 7 cut(s) 434, 568, 1022, 1578, 1997, 2617, 3327
EarI CTCTTC 7 cut(s) 434, 568, 1022, 1578, 1997, 2617, 3327
Ecl136II GAGCTC 1 cut(s) 1525
Eco24I GRGCYC 1 cut(s) 1527
Eco53kI GAGCTC 1 cut(s) 1525
Eco57I CTGAAG 4 cut(s) 257, 2061, 2723, 3584
EcoICRI GAGCTC 1 cut(s) 1525
EcoO109I RGGNCCY 1 cut(s) 1706
EcoRI GAATTC 2 cut(s) 112, 2571
EcoRII CCWGG 6 cut(s) 991, 1617, 1683, 1690, 2100, 2114
EcoT22I ATGCAT 2 cut(s) 223, 1203
EcoT38I GRGCYC 1 cut(s) 1527
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
FalI AAGNNNNNCTT 6 cut(s) 801, 833, 911, 943, 1397, 1429
FaqI GGGAC 4 cut(s) 2289, 2862, 3453, 3705
FbaI TGATCA 2 cut(s) 2253, 3403
FblI GTMKAC 1 cut(s) 1652
FokI GGATG 7 cut(s) 17, 1378, 1463, 2609, 2655, 2953, 3383
FriOI GRGCYC 1 cut(s) 1527
FspBI CTAG 9 cut(s) 948, 1146, 1158, 1220, 1229, 1983, 2193, 2445, 2579
GlaI GCGC 5 cut(s) 25, 36, 1224, 2015, 3237
GsuI CTGGAG 2 cut(s) 3136, 3369
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 6 cut(s) 782, 942, 1418, 1707, 2132, 2426
HapII CCGG 4 cut(s) 32, 50, 377, 3379
HgaI GACGC 1 cut(s) 42
HhaI GCGC 5 cut(s) 26, 37, 1225, 2016, 3238
Hin1I GRCGYC 2 cut(s) 35, 53
Hin6I GCGC 5 cut(s) 24, 35, 1223, 2014, 3236
HinP1I GCGC 5 cut(s) 24, 35, 1223, 2014, 3236
HincII GTYRAC 3 cut(s) 306, 877, 1776
HindII GTYRAC 3 cut(s) 306, 877, 1776
HindIII AAGCTT 4 cut(s) 1792, 3224, 3608, 3749
HpaI GTTAAC 1 cut(s) 877
HpaII CCGG 4 cut(s) 32, 50, 377, 3379
HphI GGTGA 8 cut(s) 21, 464, 548, 860, 1113, 1526, 1820, 2611
Hpy166II GTNNAC 5 cut(s) 306, 877, 1653, 1776, 3268
Hpy8I GTNNAC 5 cut(s) 306, 877, 1653, 1776, 3268
Hpy99I CGWCG 2 cut(s) 198, 250
HpyCH4III ACNGT 4 cut(s) 349, 1860, 3213, 3630
HpyCH4IV ACGT 6 cut(s) 43, 547, 590, 3264, 3636, 3684
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 643, 788, 2111, 2243, 3146, 3350, 3588, 3748
HpySE526I ACGT 6 cut(s) 43, 547, 590, 3264, 3636, 3684
Hsp92I GRCGYC 2 cut(s) 35, 53
HspAI GCGC 5 cut(s) 24, 35, 1223, 2014, 3236
KasI GGCGCC 1 cut(s) 34
Ksp22I TGATCA 2 cut(s) 2253, 3403
KspAI GTTAAC 1 cut(s) 877
KspI CCGCGG 1 cut(s) 95
LguI GCTCTTC 1 cut(s) 3327
LmnI GCTCC 5 cut(s) 1951, 2189, 2876, 3350, 3649
MaeI CTAG 9 cut(s) 948, 1146, 1158, 1220, 1229, 1983, 2193, 2445, 2579
MaeII ACGT 6 cut(s) 43, 547, 590, 3264, 3636, 3684
MaeIII GTNAC 8 cut(s) 60, 68, 754, 1514, 1808, 2047, 2398, 3630
MfeI CAATTG 1 cut(s) 1420
MflI RGATCY 5 cut(s) 326, 950, 1680, 1687, 3160
MhlI GDGCHC 2 cut(s) 1527, 1982
MlsI TGGCCA 2 cut(s) 1418, 2132
MluNI TGGCCA 2 cut(s) 1418, 2132
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 5 cut(s) 419, 554, 688, 1584, 3049
MmeI TCCRAC 3 cut(s) 460, 1419, 2134
Mox20I TGGCCA 2 cut(s) 1418, 2132
Mph1103I ATGCAT 2 cut(s) 223, 1203
MroXI GAANNNNTTC 2 cut(s) 3073, 3600
MscI TGGCCA 2 cut(s) 1418, 2132
MslI CAYNNNNRTG 6 cut(s) 216, 1379, 1540, 2969, 3098, 3285
Msp20I TGGCCA 2 cut(s) 1418, 2132
MspA1I CMGCKG 3 cut(s) 94, 1567, 3347
MspCI CTTAAG 1 cut(s) 1445
MspI CCGG 4 cut(s) 32, 50, 377, 3379
MspR9I CCNGG 7 cut(s) 377, 993, 1619, 1685, 1692, 2102, 2116
MunI CAATTG 1 cut(s) 1420
Mva1269I GAATGC 3 cut(s) 648, 2760, 3180
MvaI CCWGG 6 cut(s) 993, 1619, 1685, 1692, 2102, 2116
MvnI CGCG 2 cut(s) 94, 2014
MwoI GCNNNNNNNGC 9 cut(s) 32, 643, 788, 2111, 2243, 3146, 3350, 3588, 3748
NarI GGCGCC 1 cut(s) 35
NciI CCSGG 1 cut(s) 377
NlaIV GGNNCC 7 cut(s) 36, 1682, 1689, 2061, 2099, 2185, 3493
NmuCI GTSAC 3 cut(s) 68, 1514, 1808
NsiI ATGCAT 2 cut(s) 223, 1203
NspI RCATGY 2 cut(s) 2819, 3425
NspV TTCGAA 3 cut(s) 116, 2860, 3604
OliI CACNNNNGTG 1 cut(s) 3285
PaeI GCATGC 1 cut(s) 2819
PagI TCATGA 3 cut(s) 1357, 2548, 3099
PciSI GCTCTTC 1 cut(s) 3327
PctI GAATGC 3 cut(s) 648, 2760, 3180
PdmI GAANNNNTTC 2 cut(s) 3073, 3600
PflMI CCANNNNNTGG 1 cut(s) 1624
PfoI TCCNGGA 1 cut(s) 1683
PleI GAGTC 5 cut(s) 418, 554, 688, 1584, 3048
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 5 cut(s) 418, 554, 688, 1584, 3048
Ppu21I YACGTR 1 cut(s) 44
PshBI ATTAAT 1 cut(s) 1581
PsiI TTATAA 3 cut(s) 963, 2595, 3755
Psp124BI GAGCTC 1 cut(s) 1527
Psp1406I AACGTT 2 cut(s) 547, 3684
Psp6I CCWGG 6 cut(s) 991, 1617, 1683, 1690, 2100, 2114
PspGI CCWGG 6 cut(s) 991, 1617, 1683, 1690, 2100, 2114
PspN4I GGNNCC 7 cut(s) 36, 1682, 1689, 2061, 2099, 2185, 3493
PspPI GGNCC 1 cut(s) 1706
PsrI GAACNNNNNNTAC 2 cut(s) 3585, 3617
PstI CTGCAG 2 cut(s) 1337, 3190
PsuI RGATCY 5 cut(s) 326, 950, 1680, 1687, 3160
PvuII CAGCTG 2 cut(s) 1567, 3347
RsaI GTAC 4 cut(s) 100, 998, 1557, 3171
RsaNI GTAC 4 cut(s) 99, 997, 1556, 3170
RseI CAYNNNNRTG 6 cut(s) 216, 1379, 1540, 2969, 3098, 3285
SacI GAGCTC 1 cut(s) 1527
SacII CCGCGG 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 3327
Sau96I GGNCC 1 cut(s) 1706
SchI GAGTC 5 cut(s) 419, 554, 688, 1584, 3049
ScrFI CCNGG 7 cut(s) 377, 993, 1619, 1685, 1692, 2102, 2116
SduI GDGCHC 2 cut(s) 1527, 1982
SfcI CTRYAG 4 cut(s) 1333, 2439, 3186, 3354
SfoI GGCGCC 1 cut(s) 36
Sfr303I CCGCGG 1 cut(s) 95
SfuI TTCGAA 3 cut(s) 116, 2860, 3604
SgrAI CRCCGGYG 1 cut(s) 31
SgrBI CCGCGG 1 cut(s) 95
SmiMI CAYNNNNRTG 6 cut(s) 216, 1379, 1540, 2969, 3098, 3285
SmlI CTYRAG 4 cut(s) 655, 1088, 1445, 3150
SmoI CTYRAG 4 cut(s) 655, 1088, 1445, 3150
SphI GCATGC 1 cut(s) 2819
SsiI CCGC 9 cut(s) 17, 38, 92, 94, 140, 253, 387, 1327, 2290
SspDI GGCGCC 1 cut(s) 34
SspI AATATT 2 cut(s) 121, 2269
SspMI CTAG 9 cut(s) 948, 1146, 1158, 1220, 1229, 1983, 2193, 2445, 2579
SstI GAGCTC 1 cut(s) 1527
StyD4I CCNGG 7 cut(s) 375, 991, 1617, 1683, 1690, 2100, 2114
TaaI ACNGT 4 cut(s) 349, 1860, 3213, 3630
TaiI ACGT 6 cut(s) 46, 550, 593, 3267, 3639, 3687
TaqII GACCGA 1 cut(s) 216
TatI WGTACW 1 cut(s) 1555
TauI GCSGC 6 cut(s) 40, 94, 97, 256, 1330, 2292
TscAI CASTG 7 cut(s) 73, 157, 798, 2256, 2361, 3119, 3624
TseFI GTSAC 3 cut(s) 68, 1514, 1808
Tsp45I GTSAC 3 cut(s) 68, 1514, 1808
TspRI CASTG 7 cut(s) 73, 157, 798, 2256, 2361, 3119, 3624
Van91I CCANNNNNTGG 1 cut(s) 1624
Vha464I CTTAAG 1 cut(s) 1445
VspI ATTAAT 1 cut(s) 1581
XbaI TCTAGA 1 cut(s) 2192
XceI RCATGY 2 cut(s) 2819, 3425
XcmI CCANNNNNNNNNTGG 1 cut(s) 1691
XmiI GTMKAC 1 cut(s) 1652
XmnI GAANNNNTTC 2 cut(s) 3073, 3600
XspI CTAG 9 cut(s) 948, 1146, 1158, 1220, 1229, 1983, 2193, 2445, 2579
Zsp2I ATGCAT 2 cut(s) 223, 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.