MD10G1319300.v1.1

Abnormal spindle-like microcephaly-associated protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006240
Physical Location & Seq
Forward (+)
40155347 .. 40162874
7528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1319300.v1.1.491

Sequence Viewer

Length: 4284 bp
ATGGAAGGCCGAGAGCCACCGCCTTCTCCGTCGCCGTCGCCTTACAGAAACCCCTCCACTCTCTTCAAAGACATCACCAATTTCAAAACCCCGAAACCCTCCTCGCGAATCTCCAATTTCCAGTCCCCCGGCCCCAAATTCTTCACTGCCTCGAAACAAACCCCTGGTACTTCTTCGTCATTTCGCCGCAGGCCGTCTCTCGCTCCGTCAAACTCTGGCCGGATAAAGGCGGCGTCCCGGAAACTCAAGGCATTTGAGCTCGAGCAGTCGCAGTCGTCCCGCAAGGCCCAAATTCAGAAAGAACAATCGCTCAAGTCGCTGGCCAAGTCTCTCTCCGTCTGGCTCAATTTCCTCTTTCAAAACCCTACATCCTGTGGCTGCAATTTGTCCGTCGATGGAGACCGCACGGGAACGCTCCCCAAGGGAAAGAGGGATAGCGATCCCGGCAGTGCGGTTGGGGTTGATTCGGCGTGGCGGGACCCCAAGCGTCAGAGAGACTCGTCGTGGCGGGCCGTGAGCGCTGTAGCGTTTTCGAGCTCCAAGTATTCGAACCTGCGTTCTTCGTTGGAGCATGTGTGCAGTGTTGATGATTTGACGCAGAGGATGCGACTCTATCTGAGCATGGGCAGTTGCAAGGAGGTTTTCGATGCGATGACTCATGTAGCAAAGAATATTGATGAAGGGAGATTAAAGATGAAGGCACATTGTCCTTTAGTTACGGATTTTGGGTTGAAGGAGAAGGCCACTAGAATCCTCATGAGTTATAACCCAATTTGGCTTCGAATTGGATTGTACATTGTTTTTGGTGGAGATTCTTTGTTGTCTGACAGAGATGCTAATTCTGATGAAGAACTCAGGTTTCTGAAAATGATCATTGAGAAGCAATTTTTTGCACATTCGGGTCTAGCAAAAGATTATGCTTACAACAAGAAGGTTGAGGGTCTTTACAGACCGGGTTATTACGAAGCTCTGGGAAACGTCACTTTGAAGAGATTTCTGATGCTCGCTCTTATCCTTGATAGAGCGAAATGCCAGAGCAGTCTTTCTCTTAAGTATGGAATTGATGGAGTGGATGGGGGTTCTCCGTTATTGTTTACAGTTGAATCTCGTATTAAATCGAGTCATCAGGTGATCCATGATTTTCTGTCATCTGATGTCATGCTTGGAGAAGGTAATATTTCAGCACATCTGGTGGTTTTAGGATACAAAGTATCCTATCAGCAGCATCCCCTTGTTGAATATGACTTCCGAGTTACAAATTTATTTGCTGATCTTCAAGATGGGGTGCGACTGTGCAGAGCCATTCAACTTTTGCTAGATGACGCCTCTATCCTCACGGTTAAAATGGTTGTTCCAGCAGATACGCTTAAGAAGAACATGGCAAATTGTGGCATTGCCCTGCAGTACCTTAAACAGGCTGGCGTTGTTTTACATGATGAAGACGGAATGATGATTGTGGCAGATGATATTGCTGGCGGGGACAAGGAACTTACTCTTTCCTTGCTCTGGAACGTGTTTGTCCATTTGCAGCTACCGCTTCTGGTCAAGAAAACAAACTTAGCTGAGGAAATTTGCAAAATTCGTGGCAGTGTGATCAGTTTTGATTCCTCTTCATTGGAAATGATTTTAAAATGGATTCAGGCAATTTGTGAAAATTATGATTGCAAGGTTGACAGTTTTGCTTCATTGGTTGATGGAAAAGCCATATGGTGCTTGCTGGACTATTACTTTCGTAAGCAACTATCTTGTGGTCGGTCATCAAAGGATCGTAATAAATCAAGTCACGAAGAATCAATCATGTTGGCCACTGATTATTCAGATGCAGTGCACAACTTTTTATTATCCCAGAAATTGTTGACGTTATTGGGAAACTTTCCAGAGGTTCTGCAAATTAGTGACATTCTTGAATACAACGGTGCACGAAATGACCGGAGTGTGGTGATACTATTGGTGTTCCTTTCATCTCAACTTATTGTCAAGAAAAATATGGACCAATTAAATTTCCATAAACTCTTGGGTTGTGATTGCCAAAGTACAGACAGAAAATATACATGTATGGGATGCTATGTAAGACCCGAAGCAACACAGGTCGAAGAGGAAACATATGACCACCACATCGAAGATTCTGTAAGAAAGTTTAAGGCTATTCAGGCCTGGTGGCAAGATATGGCTGAAAGGAACCATAAATCTGTTGCAAAACCAACTTCTCCTACTTCGCTTAACCTATCTACGAATAAGGATAACATTAATATTGAAAAAGTAAATACTGACAAAGGCAATTATGAGGGAGGAAGTATTGTAACTCATGATATATCTACTCCTGATCTAGTTAAAGCTGCCATTGTTGTCCAAAGATATATTCGTGGGTGGCTTGCAAGGTTGAGATACATTCATGGGGTTGCTCTTGTAGATAAATCTTGCAATCGGTGCCAAGAAAATGTTGTGCGTGATCTTCAAGTAAGGGCAGAAAATACTGAGAAAAGCAATTATGAGGGAGGAAGTATTGTAACTCATGATATATCTGCTCCTGATCTAGTCAAAGCTGCCATTGTTGTGCAAAGATATATTCATAAATCTTGCAATCTGTGCCAAGAAAATATTGTGCGTGATCTTCAAGTAAGGGCAGAAAATACTGAAAAAAGCAATTATGAGGGAGGAAGTATTGTAACTCATGATATATCTGCTCCTGATCTAGTCAAAGCTGCCATTGTTATCCAAAGTATTGTAACTCATGATATATCTGCTCCTGATCTAGTCAAAGCTGCCATTGTTATCCAAAGATATATTCGTGGGTGGCTTGCAAGGTTGAGATACATTCATGGGGTTGATCTTGTAGATAAATCTTCCAATCTCTGCCAAGAAAGTGTTGCGCGTGATCTTCAAGTAAGGGCAGCAGTTAAAATCCAGCTTGCGTGGAAGAATTTTTCTTTCTGTCATTCTCTTCTCTATCAGCTTTCCGCTGCAACTAAAATTCAAAGTCATTTTCGTGGTTGGCTAGTGAGGAGGAGGTCTCATATTAAAAGGCAAGCAATAATAAAAATCCAAAGTGTATATCGAATGTCAAGATGCTGGAAGGCTTATCAGCAATACAAAATTGCAGCTGGTTCAGCCACCGTCATTCAATCTTATATACGTGGATGTTTTGCCAGGAAAGGCGCTGAAAATCATAGGCTTCTCATTGTTGCAATCCAAAGATACTTCCGTGGCTGGTTGATCAGAAGTCAATTCTCGTGTCAAAGACAGGCTGCAATAAAGATCCAAAGTGCTATTCGAGGCTTGATATGGCGTCAATCATTTCATCGTCATAGACAAGCTGCAGTGGAAATTCAACGGATAGTCAGGGGGCAGATTAGTCGAAATAGGCTCTTAGGTGCTGCTTCCCTACGCCCAATCGTCCGCAATGGTTGTCCTTTGAATAGAACAGAAGCCCTTTATATGGGTGCTGAACTAAATAAAGTTGTTTGCTCCGTGTTGAAATTGCAAAGGTGGTGGAGGAGTGTCATGTCACTAAACTTTAGAACGAATTCTGCAGTCATTATTCAGTCTCATATTCGAGGGTGGTTTGCTAGGCAAAAAGCTGTTAGAGAGAGGCAATGTATTGTTGCGATACAATCATGCTGGAGAGGCTACCTTCTAAGGAGGAAAGAGACGGAAGGTCAGCTACTGGAGCTGCGCTTGAGAGTGCAAAAGTCTGCCACAAATGTAGATGATAGCATGCGGATTATAAACAGACTTGTAGCAGCACTTTCGGAGCTACAAACAATGAAAAGTATCAGCGGCATTCTTCACACTTGTGTTACTTTGGACAAGGCCACTGCACATTCTCAGAAATGTTGTGAAAAACTCGTGGAGGAAGGAGCTATTAAAACATTGCTGAAGATTTTTGGCTCAGCCACTCGAAGTATACCCGATCAGGAGGTTCAAAAACACGTGCTCTCAACTCTAAGGAACCTTGCCCGCTATCCACACCTGGTAGAAGTGCTAATTGACAGTCCGGGATCAGTAGAAACTGTTGTATGCGAGTTTGTAAGGAACAAGGAAGAGGGTTATTTCACAGCTTCCGAACTTCTGAAGAAGGTTTGTGCTAGTCGTAAAGGCGTTGAAGCTGTGCGTAAATCGCCCGCTCTTTTGAAAAGGCTACACAATCTTGTTGAGGAACTTAGTAGGAAGGCTGGCAACAATGACAAGAGGAATGCCGGCCGAGTTACAGTTGCTAGAGAAGCTACGGAAAGGAGATTAAGGGAGGCTGTTGAAATTATGAAAATGGTAAAACAGAGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm CH IQ

Protein Analysis

1428

Amino Acids

161.23

Weight (kDa)

9.56

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 986 - 1003 6.7e-06 IQ calmodulin-binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 765, 3725
Acc36I ACCTGC 1 cut(s) 561
AccB1I GGYRCC 1 cut(s) 2427
AccBSI CCGCTC 1 cut(s) 4124
AccI GTMKAC 1 cut(s) 3904
AccII CGCG 2 cut(s) 106, 2872
AclWI GGATC 5 cut(s) 434, 1126, 1773, 3250, 4006
AcoI YGGCCR 4 cut(s) 217, 321, 1803, 4198
AcuI CTGAAG 2 cut(s) 3896, 4091
AcvI CACGTG 1 cut(s) 3931
AcyI GRCGYC 3 cut(s) 233, 1323, 3286
AfaI GTAC 5 cut(s) 169, 794, 1406, 2035, 4280
AfeI AGCGCT 1 cut(s) 520
AfiI CCNNNNNNNGG 5 cut(s) 226, 1414, 1506, 1936, 3436
AflII CTTAAG 2 cut(s) 1049, 1367
AflIII ACRYGT 3 cut(s) 1512, 2051, 3928
AhdI GACNNNNNGTC 1 cut(s) 3654
AjnI CCWGG 4 cut(s) 163, 2153, 3146, 3969
AjuI GAANNNNNNNTTGG 6 cut(s) 2343, 2375, 2769, 2801, 3252, 3284
AleI CACNNNNGTG 1 cut(s) 3792
Alw21I GWGCWC 5 cut(s) 261, 539, 1830, 1921, 3936
Alw26I GTCTC 7 cut(s) 201, 333, 393, 489, 3015, 3549, 3641
Alw44I GTGCAC 2 cut(s) 1826, 1917
AlwI GGATC 5 cut(s) 434, 1126, 1773, 3250, 4006
AlwNI CAGNNNCTG 1 cut(s) 3664
Ama87I CYCGRG 1 cut(s) 260
Aor51HI AGCGCT 1 cut(s) 520
ApaLI GTGCAC 2 cut(s) 1826, 1917
AseI ATTAAT 1 cut(s) 2247
Asp700I GAANNNNTTC 1 cut(s) 3523
AspLEI GCGC 4 cut(s) 521, 2872, 3158, 3675
AspS9I GGNCC 5 cut(s) 131, 286, 478, 510, 1990
AsuC2I CCSGG 5 cut(s) 129, 238, 444, 954, 3996
AsuHPI GGTGA 3 cut(s) 67, 1141, 1951
AsuII TTCGAA 2 cut(s) 548, 781
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 2 cut(s) 478, 1990
BaeGI GKGCMC 2 cut(s) 1830, 1921
BaeI ACNNNNGTAYC 2 cut(s) 3187, 3220
BalI TGGCCA 2 cut(s) 323, 1805
BanI GGYRCC 1 cut(s) 2427
BanII GRGCYC 2 cut(s) 261, 539
BarI GAAGNNNNNNTAC 2 cut(s) 3645, 3677
BauI CACGAG 2 cut(s) 3229, 3845
BbrPI CACGTG 1 cut(s) 3931
BbsI GAAGAC 1 cut(s) 1446
Bbv12I GWGCWC 5 cut(s) 261, 539, 1830, 1921, 3936
BbvCI CCTCAGC 1 cut(s) 1563
BccI CCATC 5 cut(s) 389, 1058, 1067, 1274, 1688
BceAI ACGGC 3 cut(s) 19, 178, 497
BcgI CGANNNNNNTGC 4 cut(s) 3335, 3369, 3729, 3763
BciT130I CCWGG 4 cut(s) 165, 2155, 3148, 3971
BciVI GTATCC 2 cut(s) 1196, 1222
BclI TGATCA 3 cut(s) 870, 1593, 3213
BcnI CCSGG 5 cut(s) 129, 238, 444, 954, 3996
BcoDI GTCTC 7 cut(s) 201, 333, 393, 489, 3015, 3549, 3641
BfmI CTRYAG 4 cut(s) 522, 1400, 3315, 3528
BfoI RGCGCY 2 cut(s) 522, 3159
BfrI CTTAAG 2 cut(s) 1049, 1367
BfuAI ACCTGC 1 cut(s) 561
BfuI GTATCC 2 cut(s) 1196, 1222
BlpI GCTNAGC 1 cut(s) 3889
Bme1390I CCNGG 9 cut(s) 129, 165, 238, 444, 954, 2155, 3148, 3971, 3996
Bme18I GGWCC 2 cut(s) 478, 1990
BmeRI GACNNNNNGTC 1 cut(s) 3654
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 5 cut(s) 131, 286, 478, 510, 1990
BmiI GGNNCC 6 cut(s) 133, 479, 480, 2180, 2429, 3950
BmrFI CCNGG 9 cut(s) 129, 165, 238, 444, 954, 2155, 3148, 3971, 3996
BmsI GCATC 8 cut(s) 594, 637, 823, 990, 1234, 1810, 2051, 3056
BpiI GAAGAC 1 cut(s) 1446
BplI GAGNNNNNCTC 2 cut(s) 2995, 3027
BpmI CTGGAG 2 cut(s) 3640, 3686
Bpu10I CCTNAGC 1 cut(s) 1563
Bpu1102I GCTNAGC 1 cut(s) 3889
Bpu14I TTCGAA 2 cut(s) 548, 781
BpuEI CTTGAG 3 cut(s) 230, 296, 3697
BpuMI CCSGG 5 cut(s) 129, 238, 444, 954, 3996
BsaAI YACGTR 2 cut(s) 3134, 3931
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 3 cut(s) 233, 1323, 3286
BsaI GGTCTC 2 cut(s) 393, 3015
BsaJI CCNNGG 4 cut(s) 127, 163, 420, 3202
BsaWI WCCGGW 1 cut(s) 1929
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 5 cut(s) 226, 1414, 1506, 1936, 3436
Bse118I RCCGGY 1 cut(s) 4196
Bse1I ACTGG 2 cut(s) 121, 3669
Bse3DI GCAATG 4 cut(s) 1392, 3406, 3599, 3869
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 4 cut(s) 165, 2155, 3148, 3971
BseDI CCNNGG 4 cut(s) 127, 163, 420, 3202
BseGI GGATG 6 cut(s) 368, 609, 1078, 1225, 2066, 3143
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 5 cut(s) 226, 1414, 1506, 1936, 3436
BseMI GCAATG 4 cut(s) 1392, 3406, 3599, 3869
BseMII CTCAG 6 cut(s) 608, 868, 1554, 2466, 3839, 3903
BseNI ACTGG 2 cut(s) 121, 3669
BseRI GAGGAG 4 cut(s) 91, 3016, 3019, 3508
BseSI GKGCMC 2 cut(s) 1830, 1921
BseX3I CGGCCG 1 cut(s) 4198
BsgI GTGCAG 3 cut(s) 598, 1315, 3801
Bsh1236I CGCG 2 cut(s) 106, 2872
Bsh1285I CGRYCG 1 cut(s) 4201
BshNI GGYRCC 1 cut(s) 2427
BsiEI CGRYCG 1 cut(s) 4201
BsiHKAI GWGCWC 5 cut(s) 261, 539, 1830, 1921, 3936
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 8 cut(s) 129, 220, 238, 444, 953, 1930, 3995, 4197
BslFI GGGAC 5 cut(s) 109, 220, 262, 491, 1493
BslI CCNNNNNNNGG 5 cut(s) 226, 1414, 1506, 1936, 3436
BsmAI GTCTC 7 cut(s) 201, 333, 393, 489, 3015, 3549, 3641
BsmBI CGTCTC 2 cut(s) 201, 3641
BsmFI GGGAC 5 cut(s) 109, 220, 262, 491, 1493
BsmI GAATGC 2 cut(s) 3780, 4198
Bso31I GGTCTC 2 cut(s) 393, 3015
BsoBI CYCGRG 1 cut(s) 260
Bsp119I TTCGAA 2 cut(s) 548, 781
Bsp1286I GDGCHC 5 cut(s) 261, 539, 1830, 1921, 3936
Bsp1407I TGTACA 1 cut(s) 792
Bsp1720I GCTNAGC 1 cut(s) 3889
Bsp68I TCGCGA 1 cut(s) 106
BspCNI CTCAG 6 cut(s) 609, 867, 1555, 2467, 3838, 3902
BspFNI CGCG 2 cut(s) 106, 2872
BspHI TCATGA 5 cut(s) 756, 2305, 2512, 2671, 2731
BspLI GGNNCC 6 cut(s) 133, 479, 480, 2180, 2429, 3950
BspMAI CTGCAG 3 cut(s) 1404, 3319, 3532
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 5 cut(s) 434, 1126, 1773, 3250, 4006
BspT104I TTCGAA 2 cut(s) 548, 781
BspT107I GGYRCC 1 cut(s) 2427
BspTI CTTAAG 2 cut(s) 1049, 1367
BspTNI GGTCTC 2 cut(s) 393, 3015
BsrBI CCGCTC 1 cut(s) 4124
BsrDI GCAATG 4 cut(s) 1392, 3406, 3599, 3869
BsrFI RCCGGY 1 cut(s) 4196
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 2 cut(s) 121, 3669
BssAI RCCGGY 1 cut(s) 4196
BssECI CCNNGG 4 cut(s) 127, 163, 420, 3202
BssNAI GTATAC 1 cut(s) 3905
BssNI GRCGYC 3 cut(s) 233, 1323, 3286
BssSI CACGAG 2 cut(s) 3229, 3845
BssT1I CCWWGG 1 cut(s) 420
Bst1107I GTATAC 1 cut(s) 3905
Bst2BI CACGAG 2 cut(s) 3229, 3845
Bst2UI CCWGG 4 cut(s) 165, 2155, 3148, 3971
Bst4CI ACNGT 9 cut(s) 1099, 1293, 1339, 1676, 1916, 3115, 3992, 4012, 4210
Bst6I CTCTTC 6 cut(s) 68, 983, 1615, 2088, 2946, 4035
BstACI GRCGYC 3 cut(s) 233, 1323, 3286
BstAFI CTTAAG 2 cut(s) 1049, 1367
BstAPI GCANNNNNTGC 3 cut(s) 604, 2427, 2586
BstAUI TGTACA 1 cut(s) 792
BstBAI YACGTR 2 cut(s) 3134, 3931
BstBI TTCGAA 2 cut(s) 548, 781
BstDSI CCRYGG 1 cut(s) 3202
BstENI CCTNNNNNAGG 1 cut(s) 1412
BstF5I GGATG 6 cut(s) 368, 609, 1078, 1225, 2066, 3143
BstFNI CGCG 2 cut(s) 106, 2872
BstH2I RGCGCY 2 cut(s) 522, 3159
BstHHI GCGC 4 cut(s) 521, 2872, 3158, 3675
BstMAI GTCTC 7 cut(s) 201, 333, 393, 489, 3015, 3549, 3641
BstMCI CGRYCG 1 cut(s) 4201
BstNI CCWGG 4 cut(s) 165, 2155, 3148, 3971
BstNSI RCATGY 3 cut(s) 575, 2055, 3718
BstSCI CCNGG 9 cut(s) 127, 163, 236, 442, 952, 2153, 3146, 3969, 3994
BstSFI CTRYAG 4 cut(s) 522, 1400, 3315, 3528
BstSLI GKGCMC 2 cut(s) 1830, 1921
BstUI CGCG 2 cut(s) 106, 2872
BstV2I GAAGAC 1 cut(s) 1446
BstX2I RGATCY 1 cut(s) 3255
BstYI RGATCY 1 cut(s) 3255
BstZ17I GTATAC 1 cut(s) 3905
BstZI CGGCCG 1 cut(s) 4198
BsuI GTATCC 2 cut(s) 1196, 1222
BtgI CCRYGG 1 cut(s) 3202
BtgZI GCGATG 1 cut(s) 665
BtsCI GGATG 6 cut(s) 368, 609, 1078, 1225, 2066, 3143
BtsI GCAGTG 7 cut(s) 144, 454, 586, 1594, 1830, 3324, 3813
BtsIMutI CAGTG 8 cut(s) 144, 454, 586, 1594, 1806, 1830, 3324, 3813
BtuMI TCGCGA 1 cut(s) 106
BveI ACCTGC 1 cut(s) 561
CaiI CAGNNNCTG 1 cut(s) 3664
CciI TCATGA 5 cut(s) 756, 2305, 2512, 2671, 2731
CfoI GCGC 4 cut(s) 521, 2872, 3158, 3675
Cfr10I RCCGGY 1 cut(s) 4196
Cfr13I GGNCC 5 cut(s) 131, 286, 478, 510, 1990
CseI GACGC 5 cut(s) 222, 476, 604, 1331, 3275
CsiI ACCWGGT 1 cut(s) 3969
Csp6I GTAC 5 cut(s) 168, 793, 1405, 2034, 4279
CspCI CAANNNNNGTGG 4 cut(s) 1564, 1599, 3470, 3505
CviQI GTAC 5 cut(s) 168, 793, 1405, 2034, 4279
DraI TTTAAA 1 cut(s) 1629
DriI GACNNNNNGTC 1 cut(s) 3654
EaeI YGGCCR 4 cut(s) 217, 321, 1803, 4198
EagI CGGCCG 1 cut(s) 4198
Eam1104I CTCTTC 6 cut(s) 68, 983, 1615, 2088, 2946, 4035
Eam1105I GACNNNNNGTC 1 cut(s) 3654
EarI CTCTTC 6 cut(s) 68, 983, 1615, 2088, 2946, 4035
Ecl136II GAGCTC 2 cut(s) 259, 537
EclXI CGGCCG 1 cut(s) 4198
Eco130I CCWWGG 1 cut(s) 420
Eco147I AGGCCT 1 cut(s) 2153
Eco24I GRGCYC 2 cut(s) 261, 539
Eco31I GGTCTC 2 cut(s) 393, 3015
Eco47I GGWCC 2 cut(s) 478, 1990
Eco47III AGCGCT 1 cut(s) 520
Eco52I CGGCCG 1 cut(s) 4198
Eco53kI GAGCTC 2 cut(s) 259, 537
Eco57I CTGAAG 2 cut(s) 3896, 4091
Eco72I CACGTG 1 cut(s) 3931
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 2 cut(s) 259, 537
EcoNI CCTNNNNNAGG 1 cut(s) 1412
EcoO109I RGGNCCY 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 3523
EcoRII CCWGG 4 cut(s) 163, 2153, 3146, 3969
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 2 cut(s) 261, 539
ErhI CCWWGG 1 cut(s) 420
Esp3I CGTCTC 2 cut(s) 201, 3641
FaqI GGGAC 5 cut(s) 109, 220, 262, 491, 1493
FauI CCCGC 6 cut(s) 287, 468, 501, 1469, 3965, 4129
FauNDI CATATG 2 cut(s) 1706, 2104
FbaI TGATCA 3 cut(s) 870, 1593, 3213
FblI GTMKAC 1 cut(s) 3904
FokI GGATG 6 cut(s) 355, 616, 1085, 1212, 2073, 3150
FriOI GRGCYC 2 cut(s) 261, 539
GlaI GCGC 4 cut(s) 520, 2871, 3157, 3674
GsuI CTGGAG 2 cut(s) 3640, 3686
HaeII RGCGCY 2 cut(s) 522, 3159
HapII CCGG 8 cut(s) 129, 220, 238, 444, 953, 1930, 3995, 4197
HgaI GACGC 5 cut(s) 222, 476, 604, 1331, 3275
HhaI GCGC 4 cut(s) 521, 2872, 3158, 3675
Hin1I GRCGYC 3 cut(s) 233, 1323, 3286
Hin6I GCGC 4 cut(s) 519, 2870, 3156, 3673
HinP1I GCGC 4 cut(s) 519, 2870, 3156, 3673
HincII GTYRAC 2 cut(s) 1672, 1857
HindII GTYRAC 2 cut(s) 1672, 1857
HpaII CCGG 8 cut(s) 129, 220, 238, 444, 953, 1930, 3995, 4197
HphI GGTGA 3 cut(s) 67, 1141, 1951
Hpy166II GTNNAC 6 cut(s) 1095, 1672, 1828, 1857, 1919, 3905
Hpy8I GTNNAC 6 cut(s) 1095, 1672, 1828, 1857, 1919, 3905
Hpy99I CGWCG 4 cut(s) 34, 40, 395, 505
HpyCH4III ACNGT 9 cut(s) 1099, 1293, 1339, 1676, 1916, 3115, 3992, 4012, 4210
HpyCH4IV ACGT 5 cut(s) 978, 1512, 1859, 3133, 3930
HpySE526I ACGT 5 cut(s) 978, 1512, 1859, 3133, 3930
Hsp92I GRCGYC 3 cut(s) 233, 1323, 3286
HspAI GCGC 4 cut(s) 519, 2870, 3156, 3673
KflI GGGWCCC 1 cut(s) 478
KroI GCCGGC 1 cut(s) 4196
KroNI GCCGGC 1 cut(s) 4198
Ksp22I TGATCA 3 cut(s) 870, 1593, 3213
LweI GCATC 8 cut(s) 594, 637, 823, 990, 1234, 1810, 2051, 3056
MabI ACCWGGT 1 cut(s) 3969
MaeII ACGT 5 cut(s) 978, 1512, 1859, 3133, 3930
MbiI CCGCTC 1 cut(s) 4124
MflI RGATCY 1 cut(s) 3255
MhlI GDGCHC 5 cut(s) 261, 539, 1830, 1921, 3936
MlsI TGGCCA 2 cut(s) 323, 1805
MluNI TGGCCA 2 cut(s) 323, 1805
MlyI GAGTC 4 cut(s) 491, 603, 649, 1129
MmeI TCCRAC 1 cut(s) 546
Mox20I TGGCCA 2 cut(s) 323, 1805
MroNI GCCGGC 1 cut(s) 4196
MroXI GAANNNNTTC 1 cut(s) 3523
MscI TGGCCA 2 cut(s) 323, 1805
MslI CAYNNNNRTG 3 cut(s) 2552, 2985, 3792
Msp20I TGGCCA 2 cut(s) 323, 1805
MspA1I CMGCKG 3 cut(s) 2960, 3101, 3777
MspCI CTTAAG 2 cut(s) 1049, 1367
MspI CCGG 8 cut(s) 129, 220, 238, 444, 953, 1930, 3995, 4197
MspR9I CCNGG 9 cut(s) 129, 165, 238, 444, 954, 2155, 3148, 3971, 3996
Mva1269I GAATGC 2 cut(s) 3780, 4198
MvaI CCWGG 4 cut(s) 165, 2155, 3148, 3971
MvnI CGCG 2 cut(s) 106, 2872
NaeI GCCGGC 1 cut(s) 4198
NciI CCSGG 5 cut(s) 129, 238, 444, 954, 3996
NdeI CATATG 2 cut(s) 1706, 2104
NgoMIV GCCGGC 1 cut(s) 4196
NlaIV GGNNCC 6 cut(s) 133, 479, 480, 2180, 2429, 3950
NmeAIII GCCGAG 2 cut(s) 35, 4226
NmuCI GTSAC 4 cut(s) 979, 1781, 1895, 3504
NruI TCGCGA 1 cut(s) 106
NspI RCATGY 3 cut(s) 575, 2055, 3718
NspV TTCGAA 2 cut(s) 548, 781
OliI CACNNNNGTG 1 cut(s) 3792
PaeI GCATGC 1 cut(s) 3718
PaeR7I CTCGAG 1 cut(s) 260
PagI TCATGA 5 cut(s) 756, 2305, 2512, 2671, 2731
PceI AGGCCT 1 cut(s) 2153
PciI ACATGT 1 cut(s) 2051
PctI GAATGC 2 cut(s) 3780, 4198
PdiI GCCGGC 1 cut(s) 4198
PdmI GAANNNNTTC 1 cut(s) 3523
PfeI GAWTC 9 cut(s) 108, 464, 750, 812, 1103, 1604, 1636, 1790, 2123
PfoI TCCNGGA 2 cut(s) 236, 3994
PleI GAGTC 4 cut(s) 491, 603, 649, 1128
PmaCI CACGTG 1 cut(s) 3931
PmlI CACGTG 1 cut(s) 3931
PpsI GAGTC 4 cut(s) 491, 603, 649, 1128
Ppu21I YACGTR 2 cut(s) 3134, 3931
PpuMI RGGWCCY 1 cut(s) 478
PscI ACATGT 1 cut(s) 2051
PshBI ATTAAT 1 cut(s) 2247
PsiI TTATAA 2 cut(s) 765, 3725
Psp124BI GAGCTC 2 cut(s) 261, 539
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 4 cut(s) 163, 2153, 3146, 3969
PspCI CACGTG 1 cut(s) 3931
PspGI CCWGG 4 cut(s) 163, 2153, 3146, 3969
PspN4I GGNNCC 6 cut(s) 133, 479, 480, 2180, 2429, 3950
PspPI GGNCC 5 cut(s) 131, 286, 478, 510, 1990
PspPPI RGGWCCY 1 cut(s) 478
PspXI VCTCGAGB 1 cut(s) 260
PstI CTGCAG 3 cut(s) 1404, 3319, 3532
PstNI CAGNNNCTG 1 cut(s) 3664
PsuI RGATCY 1 cut(s) 3255
PvuII CAGCTG 1 cut(s) 3101
RruI TCGCGA 1 cut(s) 106
RsaI GTAC 5 cut(s) 169, 794, 1406, 2035, 4280
RsaNI GTAC 5 cut(s) 168, 793, 1405, 2034, 4279
RseI CAYNNNNRTG 3 cut(s) 2552, 2985, 3792
SacI GAGCTC 2 cut(s) 261, 539
Sau96I GGNCC 5 cut(s) 131, 286, 478, 510, 1990
SchI GAGTC 4 cut(s) 491, 603, 649, 1129
ScrFI CCNGG 9 cut(s) 129, 165, 238, 444, 954, 2155, 3148, 3971, 3996
SduI GDGCHC 5 cut(s) 261, 539, 1830, 1921, 3936
SexAI ACCWGGT 1 cut(s) 3969
SfaNI GCATC 8 cut(s) 594, 637, 823, 990, 1234, 1810, 2051, 3056
SfcI CTRYAG 4 cut(s) 522, 1400, 3315, 3528
Sfr274I CTCGAG 1 cut(s) 260
SfuI TTCGAA 2 cut(s) 548, 781
SinI GGWCC 2 cut(s) 478, 1990
SlaI CTCGAG 1 cut(s) 260
SmiMI CAYNNNNRTG 3 cut(s) 2552, 2985, 3792
SmlI CTYRAG 6 cut(s) 245, 260, 311, 1049, 1367, 3676
SmoI CTYRAG 6 cut(s) 245, 260, 311, 1049, 1367, 3676
SphI GCATGC 1 cut(s) 3718
SseBI AGGCCT 1 cut(s) 2153
SspI AATATT 4 cut(s) 673, 1177, 2251, 2599
SstI GAGCTC 2 cut(s) 261, 539
StuI AGGCCT 1 cut(s) 2153
StyD4I CCNGG 9 cut(s) 127, 163, 236, 442, 952, 2153, 3146, 3969, 3994
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 9 cut(s) 1099, 1293, 1339, 1676, 1916, 3115, 3992, 4012, 4210
TaiI ACGT 5 cut(s) 981, 1515, 1862, 3136, 3933
TaqII GACCGA 1 cut(s) 1743
TatI WGTACW 2 cut(s) 792, 2033
TauI GCSGC 3 cut(s) 189, 233, 3780
TfiI GAWTC 9 cut(s) 108, 464, 750, 812, 1103, 1604, 1636, 1790, 2123
TscAI CASTG 8 cut(s) 151, 454, 586, 1594, 1813, 1830, 3324, 3820
TseFI GTSAC 4 cut(s) 979, 1781, 1895, 3504
Tsp45I GTSAC 4 cut(s) 979, 1781, 1895, 3504
TspRI CASTG 8 cut(s) 151, 454, 586, 1594, 1813, 1830, 3324, 3820
Vha464I CTTAAG 2 cut(s) 1049, 1367
VneI GTGCAC 2 cut(s) 1826, 1917
VpaK11BI GGWCC 2 cut(s) 478, 1990
VspI ATTAAT 1 cut(s) 2247
XagI CCTNNNNNAGG 1 cut(s) 1412
XceI RCATGY 3 cut(s) 575, 2055, 3718
XhoI CTCGAG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 3904
XmnI GAANNNNTTC 1 cut(s) 3523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.