Rorug04G0404400

Abnormal spindle-like microcephaly-associated protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006240
Physical Location & Seq
Reverse (-)
56850147 .. 56853894
3748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0404400.1

Sequence Viewer

Length: 1617 bp
ATGGGGGAGGTTAGAGAAGAAAATAAAAGGCTAAAGGTGTTATTAGCGCAAATCGTGAAGGATTACCAGGAACTTCAGATGCATTTTCTTGATGTTATTCAAAAACAAGAAACAAATAAGAAATCTATGGATACCAGTAGTACAGCTGATCATCAAGGAACTGTTTCGGCTGAAGCTGATGAACTTGTGTCCCTTAGCCTTGGGAGAACCTCGAGTAGTACTAGTACTCATGATCATCATCTCAGAAAGGATCATCATGAGATGATGAAAAAGACCAGCAGAGATGACGATTACGGGGTAATGAATGAAGCTGGACTTGCATTGGAATTGGGATGCAGATCATTTGAGCCAGCTGCTGCTGATCATGAAACTACGAAGGTTAATTCAAGCTCCGAAATTAATAGTTCAGCCGCAGATCCAAAGGAAGACGAAGTAACTAAGATATGGCCGCCTAGTAAAATGTTGAAGACAAGAGATGATGATGTTTCACAACAGCAGACCCATTTGAAGAAAGCTAGGGTTTCTGTCCGAGCTAGATGCGATGCTCCAACATTGAATGATGGATGTCAATGGAGAAAATATGGACAGAAAATAGCAAAAGGAAATCCATGCCCTCGAGCATACTACCGTTGCACAGTTTCACCGTCGTGCCCTGTGAGAAAACAAGTGCAAAGATGTGCGGAAGACATGTCGATATTGATCACAACCTATGAGGGTAACCACAACCATCCACTTCCCATGTCAGCCACGGCCATGGCCTCCACCACCTCCGCCGCGGCATCCATGCTTCAGTCTCACTCATCCACCTCGCAACAAGGCCTTGTCAACTCAACCACCGCCCCCATCTCCACCTCCACTAGTAACCTCCATGGCCATGGACCAAACTTCACCAATAGTCTCTCCCAGAATTCATCGAGACTACTACCACAGTCACAGTTCTATTTCCCCAACAGTTCAATCTCAACCAACAATTCCCACCCAACCATCACCCTTGATCTCACTGCTCCATCTCCCTCCCATTTCGGACTAAGATTTCCTTCAGGGTTACCGAGATATTCCTCGAGTACAAGCCTCAACTTTTCGTCATCGTCTTCCTCTTCTTTCGACCACAACACATTACTACAAGCACCAAATTGGATTAACAATCCCGCTGCTGCAGGCTATTTCAACTATGGGACGGTACTATCTCATCGGAATAGGATGAACCAAGCTGTTGGGGGGTCTTCTCTTAACAACGGAAAGCAGCCATTTCAAGAACCTAATTTCTACCAATCTTACATACAAAATTCTCAGAAAGCAGCTCCTGCTGCACCTCAGCATCATCTGTTTACAGAGACATTAGCCACTGCAACAAAAGCAATCACATCAAACCCTACGTTTCAATCAGCTTTAGCAGCGGCACTCACGTCATTTGTTGGTGCTAATGGGGGTACTAGAGGGGCGGTCAGAGAAACTAGTCACATCGTTAATGGCAGTAGTAGTACTGAAAGTTCTGGGCTGAAATTGAAGTGGGGTGAGTCCTTGACGCCGATTAATCCAATCTACCCATCAACCCAGAAAGGAATCGGGTGCGCATCACCAGGCTACTTGAACAAATCATCGTCGCCATTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm CH IQ

Protein Analysis

538

Amino Acids

58.33

Weight (kDa)

8.75

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 186 - 244 1.5e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1573
AccII CGCG 1 cut(s) 776
AclWI GGATC 2 cut(s) 258, 410
AcoI YGGCCR 3 cut(s) 446, 750, 871
AcsI RAATTY 2 cut(s) 907, 1285
AcuI CTGAAG 4 cut(s) 59, 192, 773, 1023
AcyI GRCGYC 1 cut(s) 1526
AfaI GTAC 7 cut(s) 142, 220, 226, 1066, 1182, 1432, 1483
AflIII ACRYGT 1 cut(s) 687
AhlI ACTAGT 3 cut(s) 221, 857, 1454
AjiI CACGTC 1 cut(s) 1407
AjnI CCWGG 2 cut(s) 66, 1579
AleI CACNNNNGTG 1 cut(s) 646
Alw26I GTCTC 4 cut(s) 798, 902, 910, 1328
AlwI GGATC 2 cut(s) 258, 410
AlwNI CAGNNNCTG 2 cut(s) 356, 1304
Ama87I CYCGRG 3 cut(s) 211, 615, 1060
AoxI GGCC 5 cut(s) 446, 750, 756, 817, 871
ApeKI GCWGC 8 cut(s) 353, 356, 1151, 1154, 1243, 1298, 1307, 1394
ApoI RAATTY 2 cut(s) 907, 1285
AseI ATTAAT 2 cut(s) 399, 1533
Asp700I GAANNNNTTC 1 cut(s) 163
AspLEI GCGC 2 cut(s) 49, 1574
AspS9I GGNCC 1 cut(s) 878
AsuHPI GGTGA 5 cut(s) 633, 880, 979, 1526, 1569
AvaI CYCGRG 3 cut(s) 211, 615, 1060
AvaII GGWCC 1 cut(s) 878
BaeGI GKGCMC 1 cut(s) 653
BalI TGGCCA 1 cut(s) 873
BbsI GAAGAC 5 cut(s) 432, 473, 690, 1083, 1215
BbvCI CCTCAGC 1 cut(s) 1314
BbvI GCAGC 8 cut(s) 340, 343, 1138, 1141, 1255, 1294, 1310, 1406
BccI CCATC 6 cut(s) 554, 735, 851, 992, 1015, 1555
BceAI ACGGC 1 cut(s) 765
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BciT130I CCWGG 2 cut(s) 68, 1581
BciVI GTATCC 1 cut(s) 124
BclI TGATCA 4 cut(s) 148, 232, 361, 699
BcoDI GTCTC 4 cut(s) 798, 902, 910, 1328
BcuI ACTAGT 3 cut(s) 221, 857, 1454
BfaI CTAG 7 cut(s) 222, 453, 516, 534, 858, 1434, 1455
BfmI CTRYAG 1 cut(s) 1155
BfuI GTATCC 1 cut(s) 124
BmcAI AGTACT 3 cut(s) 220, 226, 1483
Bme1390I CCNGG 2 cut(s) 68, 1581
Bme18I GGWCC 1 cut(s) 878
BmeT110I CYCGRG 3 cut(s) 211, 615, 1060
BmgBI CACGTC 1 cut(s) 1407
BmgT120I GGNCC 1 cut(s) 878
BmrFI CCNGG 2 cut(s) 68, 1581
BmsI GCATC 7 cut(s) 69, 323, 527, 532, 788, 1327, 1583
BpiI GAAGAC 5 cut(s) 432, 473, 690, 1083, 1215
Bpu10I CCTNAGC 2 cut(s) 194, 1314
BsaBI GATNNNNATC 3 cut(s) 237, 337, 698
BsaHI GRCGYC 1 cut(s) 1526
BsaJI CCNNGG 6 cut(s) 199, 747, 753, 774, 868, 874
Bse1I ACTGG 1 cut(s) 135
Bse8I GATNNNNATC 3 cut(s) 237, 337, 698
BseBI CCWGG 2 cut(s) 68, 1581
BseDI CCNNGG 6 cut(s) 199, 747, 753, 774, 868, 874
BseGI GGATG 6 cut(s) 338, 569, 727, 779, 800, 1206
BseJI GATNNNNATC 3 cut(s) 237, 337, 698
BseMII CTCAG 3 cut(s) 256, 1304, 1328
BseNI ACTGG 1 cut(s) 135
BseSI GKGCMC 1 cut(s) 653
BseXI GCAGC 8 cut(s) 340, 343, 1138, 1141, 1255, 1294, 1310, 1406
BsgI GTGCAG 1 cut(s) 1293
Bsh1236I CGCG 1 cut(s) 776
BshFI GGCC 5 cut(s) 448, 752, 758, 819, 873
BsiHKCI CYCGRG 3 cut(s) 211, 615, 1060
BslFI GGGAC 2 cut(s) 175, 1189
BsmAI GTCTC 4 cut(s) 798, 902, 910, 1328
BsmFI GGGAC 2 cut(s) 175, 1189
BsnI GGCC 5 cut(s) 448, 752, 758, 819, 873
BsoBI CYCGRG 3 cut(s) 211, 615, 1060
Bsp1286I GDGCHC 1 cut(s) 653
Bsp143I GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
Bsp19I CCATGG 3 cut(s) 753, 868, 874
BspANI GGCC 5 cut(s) 448, 752, 758, 819, 873
BspCNI CTCAG 3 cut(s) 255, 1303, 1327
BspFNI CGCG 1 cut(s) 776
BspHI TCATGA 3 cut(s) 229, 256, 364
BspMAI CTGCAG 1 cut(s) 1159
BspPI GGATC 2 cut(s) 258, 410
BsrI ACTGG 1 cut(s) 135
BssECI CCNNGG 6 cut(s) 199, 747, 753, 774, 868, 874
BssMI GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
BssNI GRCGYC 1 cut(s) 1526
BssT1I CCWWGG 4 cut(s) 199, 753, 868, 874
Bst2UI CCWGG 2 cut(s) 68, 1581
Bst4CI ACNGT 8 cut(s) 163, 629, 637, 645, 930, 936, 953, 1180
Bst6I CTCTTC 1 cut(s) 1102
BstACI GRCGYC 1 cut(s) 1526
BstAPI GCANNNNNTGC 1 cut(s) 1304
BstC8I GCNNGC 2 cut(s) 351, 1159
BstDEI CTNAG 6 cut(s) 194, 242, 438, 1028, 1290, 1314
BstDSI CCRYGG 5 cut(s) 747, 753, 774, 868, 874
BstEII GGTNACC 2 cut(s) 716, 1044
BstF5I GGATG 6 cut(s) 338, 569, 727, 779, 800, 1206
BstFNI CGCG 1 cut(s) 776
BstHHI GCGC 2 cut(s) 49, 1574
BstKTI GATC 8 cut(s) 151, 235, 253, 341, 364, 418, 702, 997
BstMAI GTCTC 4 cut(s) 798, 902, 910, 1328
BstMBI GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
BstMWI GCNNNNNNNGC 5 cut(s) 317, 1304, 1307, 1355, 1394
BstNI CCWGG 2 cut(s) 68, 1581
BstNSI RCATGY 1 cut(s) 691
BstPI GGTNACC 2 cut(s) 716, 1044
BstSCI CCNGG 2 cut(s) 66, 1579
BstSFI CTRYAG 1 cut(s) 1155
BstSLI GKGCMC 1 cut(s) 653
BstUI CGCG 1 cut(s) 776
BstV1I GCAGC 8 cut(s) 340, 343, 1138, 1141, 1255, 1294, 1310, 1406
BstV2I GAAGAC 5 cut(s) 432, 473, 690, 1083, 1215
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 3 cut(s) 754, 875, 1214
BstYI RGATCY 1 cut(s) 415
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 5 cut(s) 448, 752, 758, 819, 873
BtgI CCRYGG 5 cut(s) 747, 753, 774, 868, 874
BtgZI GCGATG 1 cut(s) 555
BtrI CACGTC 1 cut(s) 1407
BtsCI GGATG 6 cut(s) 338, 569, 727, 779, 800, 1206
BtsI GCAGTG 2 cut(s) 999, 1344
BtsIMutI CAGTG 2 cut(s) 999, 1344
Cac8I GCNNGC 2 cut(s) 351, 1159
CaiI CAGNNNCTG 2 cut(s) 356, 1304
CciI TCATGA 3 cut(s) 229, 256, 364
CfoI GCGC 2 cut(s) 49, 1574
Cfr13I GGNCC 1 cut(s) 878
Cfr42I CCGCGG 1 cut(s) 777
CseI GACGC 1 cut(s) 1534
Csp6I GTAC 7 cut(s) 141, 219, 225, 1065, 1181, 1431, 1482
CviQI GTAC 7 cut(s) 141, 219, 225, 1065, 1181, 1431, 1482
DdeI CTNAG 6 cut(s) 194, 242, 438, 1028, 1290, 1314
DpnI GATC 8 cut(s) 150, 234, 252, 340, 363, 417, 701, 996
DpnII GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
EaeI YGGCCR 3 cut(s) 446, 750, 871
Eam1104I CTCTTC 1 cut(s) 1102
EarI CTCTTC 1 cut(s) 1102
EciI GGCGGA 1 cut(s) 760
Eco130I CCWWGG 4 cut(s) 199, 753, 868, 874
Eco147I AGGCCT 1 cut(s) 819
Eco47I GGWCC 1 cut(s) 878
Eco57I CTGAAG 4 cut(s) 59, 192, 773, 1023
Eco88I CYCGRG 3 cut(s) 211, 615, 1060
Eco91I GGTNACC 2 cut(s) 716, 1044
EcoO65I GGTNACC 2 cut(s) 716, 1044
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 66, 1579
EcoT14I CCWWGG 4 cut(s) 199, 753, 868, 874
EcoT22I ATGCAT 1 cut(s) 84
ErhI CCWWGG 4 cut(s) 199, 753, 868, 874
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 1021, 1053
FaqI GGGAC 2 cut(s) 175, 1189
FauI CCCGC 1 cut(s) 1156
FbaI TGATCA 4 cut(s) 148, 232, 361, 699
FokI GGATG 6 cut(s) 345, 576, 714, 766, 787, 1213
FspAI RTGCGCAY 1 cut(s) 1573
FspBI CTAG 7 cut(s) 222, 453, 516, 534, 858, 1434, 1455
FspI TGCGCA 1 cut(s) 1573
GlaI GCGC 2 cut(s) 48, 1573
HaeIII GGCC 5 cut(s) 448, 752, 758, 819, 873
HgaI GACGC 1 cut(s) 1534
HhaI GCGC 2 cut(s) 49, 1574
Hin1I GRCGYC 1 cut(s) 1526
Hin6I GCGC 2 cut(s) 47, 1572
HinP1I GCGC 2 cut(s) 47, 1572
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HinfI GANTC 2 cut(s) 1517, 1563
HphI GGTGA 5 cut(s) 633, 880, 979, 1526, 1569
Hpy166II GTNNAC 2 cut(s) 826, 1329
Hpy188I TCNGA 8 cut(s) 78, 245, 394, 530, 1025, 1194, 1293, 1448
Hpy188III TCNNGA 7 cut(s) 55, 89, 230, 257, 365, 915, 1253
Hpy8I GTNNAC 2 cut(s) 826, 1329
Hpy99I CGWCG 2 cut(s) 649, 1606
HpyAV CCTTC 3 cut(s) 52, 370, 1047
HpyCH4III ACNGT 8 cut(s) 163, 629, 637, 645, 930, 936, 953, 1180
HpyCH4IV ACGT 2 cut(s) 1376, 1406
HpyCH4V TGCA 8 cut(s) 82, 320, 336, 633, 670, 1157, 1310, 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 317, 1304, 1307, 1355, 1394
HpyF3I CTNAG 6 cut(s) 194, 242, 438, 1028, 1290, 1314
HpySE526I ACGT 2 cut(s) 1376, 1406
Hsp92I GRCGYC 1 cut(s) 1526
HspAI GCGC 2 cut(s) 47, 1572
Ksp22I TGATCA 4 cut(s) 148, 232, 361, 699
KspI CCGCGG 1 cut(s) 777
Kzo9I GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
LmnI GCTCC 4 cut(s) 395, 550, 1009, 1306
Lsp1109I GCAGC 8 cut(s) 340, 343, 1138, 1141, 1255, 1294, 1310, 1406
LweI GCATC 7 cut(s) 69, 323, 527, 532, 788, 1327, 1583
MaeI CTAG 7 cut(s) 222, 453, 516, 534, 858, 1434, 1455
MaeII ACGT 2 cut(s) 1376, 1406
MaeIII GTNAC 6 cut(s) 433, 716, 860, 930, 1044, 1457
MalI GATC 8 cut(s) 150, 234, 252, 340, 363, 417, 701, 996
MboI GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
MboII GAAGA 8 cut(s) 29, 437, 478, 520, 695, 1083, 1089, 1215
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 653
MlsI TGGCCA 1 cut(s) 873
MluNI TGGCCA 1 cut(s) 873
MlyI GAGTC 1 cut(s) 1526
MmeI TCCRAC 1 cut(s) 572
Mox20I TGGCCA 1 cut(s) 873
Mph1103I ATGCAT 1 cut(s) 84
MroXI GAANNNNTTC 1 cut(s) 163
MscI TGGCCA 1 cut(s) 873
MseI TTAA 6 cut(s) 381, 399, 1140, 1230, 1467, 1533
MslI CAYNNNNRTG 3 cut(s) 646, 752, 873
Msp20I TGGCCA 1 cut(s) 873
MspA1I CMGCKG 5 cut(s) 146, 353, 776, 1151, 1397
MspR9I CCNGG 2 cut(s) 68, 1581
MvaI CCWGG 2 cut(s) 68, 1581
MvnI CGCG 1 cut(s) 776
MwoI GCNNNNNNNGC 5 cut(s) 317, 1304, 1307, 1355, 1394
NcoI CCATGG 3 cut(s) 753, 868, 874
NdeII GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
NmuCI GTSAC 2 cut(s) 930, 1457
NsbI TGCGCA 1 cut(s) 1573
NsiI ATGCAT 1 cut(s) 84
NspI RCATGY 1 cut(s) 691
OliI CACNNNNGTG 1 cut(s) 646
PaeR7I CTCGAG 3 cut(s) 211, 615, 1060
PagI TCATGA 3 cut(s) 229, 256, 364
PceI AGGCCT 1 cut(s) 819
PciI ACATGT 1 cut(s) 687
PdmI GAANNNNTTC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 1563
PleI GAGTC 1 cut(s) 1525
PpsI GAGTC 1 cut(s) 1525
PscI ACATGT 1 cut(s) 687
PshBI ATTAAT 2 cut(s) 399, 1533
Psp6I CCWGG 2 cut(s) 66, 1579
PspEI GGTNACC 2 cut(s) 716, 1044
PspGI CCWGG 2 cut(s) 66, 1579
PspPI GGNCC 1 cut(s) 878
PspXI VCTCGAGB 3 cut(s) 211, 615, 1060
PsrI GAACNNNNNNTAC 2 cut(s) 1474, 1506
PstI CTGCAG 1 cut(s) 1159
PstNI CAGNNNCTG 2 cut(s) 356, 1304
PsuI RGATCY 1 cut(s) 415
PvuII CAGCTG 2 cut(s) 146, 353
RsaI GTAC 7 cut(s) 142, 220, 226, 1066, 1182, 1432, 1483
RsaNI GTAC 7 cut(s) 141, 219, 225, 1065, 1181, 1431, 1482
RseI CAYNNNNRTG 3 cut(s) 646, 752, 873
SacII CCGCGG 1 cut(s) 777
SaqAI TTAA 6 cut(s) 381, 399, 1140, 1230, 1467, 1533
Sau3AI GATC 8 cut(s) 148, 232, 250, 338, 361, 415, 699, 994
Sau96I GGNCC 1 cut(s) 878
ScaI AGTACT 3 cut(s) 220, 226, 1483
SchI GAGTC 1 cut(s) 1526
ScrFI CCNGG 2 cut(s) 68, 1581
SduI GDGCHC 1 cut(s) 653
SfaNI GCATC 7 cut(s) 69, 323, 527, 532, 788, 1327, 1583
SfcI CTRYAG 1 cut(s) 1155
Sfr274I CTCGAG 3 cut(s) 211, 615, 1060
Sfr303I CCGCGG 1 cut(s) 777
SgrBI CCGCGG 1 cut(s) 777
SinI GGWCC 1 cut(s) 878
SlaI CTCGAG 3 cut(s) 211, 615, 1060
SmiMI CAYNNNNRTG 3 cut(s) 646, 752, 873
SmlI CTYRAG 3 cut(s) 211, 615, 1060
SmoI CTYRAG 3 cut(s) 211, 615, 1060
SpeI ACTAGT 3 cut(s) 221, 857, 1454
SseBI AGGCCT 1 cut(s) 819
SspMI CTAG 7 cut(s) 222, 453, 516, 534, 858, 1434, 1455
StuI AGGCCT 1 cut(s) 819
StyD4I CCNGG 2 cut(s) 66, 1579
StyI CCWWGG 4 cut(s) 199, 753, 868, 874
TaaI ACNGT 8 cut(s) 163, 629, 637, 645, 930, 936, 953, 1180
TaiI ACGT 2 cut(s) 1379, 1409
TaqI TCGA 6 cut(s) 212, 616, 692, 914, 1061, 1104
TatI WGTACW 5 cut(s) 140, 218, 224, 1064, 1481
TauI GCSGC 5 cut(s) 413, 451, 776, 779, 1400
TfiI GAWTC 1 cut(s) 1563
Tru1I TTAA 6 cut(s) 381, 399, 1140, 1230, 1467, 1533
Tru9I TTAA 6 cut(s) 381, 399, 1140, 1230, 1467, 1533
TscAI CASTG 2 cut(s) 1006, 1351
TseFI GTSAC 2 cut(s) 930, 1457
TseI GCWGC 8 cut(s) 353, 356, 1151, 1154, 1243, 1298, 1307, 1394
Tsp45I GTSAC 2 cut(s) 930, 1457
TspDTI ATGAA 7 cut(s) 195, 281, 317, 321, 381, 900, 1217
TspGWI ACGGA 1 cut(s) 1251
TspRI CASTG 2 cut(s) 1006, 1351
VpaK11BI GGWCC 1 cut(s) 878
VspI ATTAAT 2 cut(s) 399, 1533
XapI RAATTY 2 cut(s) 907, 1285
XceI RCATGY 1 cut(s) 691
XhoI CTCGAG 3 cut(s) 211, 615, 1060
XmnI GAANNNNTTC 1 cut(s) 163
XspI CTAG 7 cut(s) 222, 453, 516, 534, 858, 1434, 1455
ZrmI AGTACT 3 cut(s) 220, 226, 1483
Zsp2I ATGCAT 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.