FvH4_3g02340

Abnormal spindle-like microcephaly-associated protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006240
Physical Location & Seq
Reverse (-)
1184010 .. 1192131
8122 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02340.t3

Sequence Viewer

Length: 4149 bp
ATGGACGCCGCCGAACCGCCGCCGTCACCGTCACCGTCGCCGTCACGTTACAAACCCCCCTCCACACTCTTCAAAGACATCTCCAATTTCAAAACCCCAAAACGCCCCTCCCGAATCTCCGAATTCCACTCCCCTTGCCCCCAATTCTTCACCGCCGCCAAACAAACCCCCCGGACAAACTCGTCATTTCGCCGCCGCCCTCCGCCGTCCTCCGCCCGCACCAACGCCGTCCGCAAACTCAAGGCCGTGGAGCTCGAGCAGTCACAGTCCTCGCGCAAGGTCCAAATCCGGAAGGAGCAGTCTCTGAAGTCTCTCGAGAAATCTCTCACCGTCTGGCTCAATTTCCTCTTCGAAAACCCTAGATCCTGCGGCTGTAACTTTCCGGTCGATGAGGATCACGGCCAAACGCTCCGGAAGGGGAAGAGGGACAGCGGCTCCGGCGTCCAGGTCCGGGTCGACGCGGCGTGGAGGGATCCGAAGCGGCAGAGAGACTCGTCATGGAGAGGTGAGGATTCCGAAGGTGCGGTTGCGTTTTCGATCTCCAAGTACTCGAAATTGCAGAGTTCTTTGAAGCTTTTGTGTAGTTTCGATGATTTGATTCTGCGAATGCGGTTTCATTTGAGCCTGGGGAGTTGCCAGGAGGTTTTCGACTCGATGATTCAAGTCGTCAAGAATATTGATGAAGGGAGGTTAAAGATGAAGGTGCATTGCCCTTTAGTGACCGATGTTGGACTGAGAAAGAAGGCCACTAAAGTTCTTTTGAGTTATAACCCAATCTGGCTTCGAATTGGACTGTACATTATATTCGGTGGCGATTCTTTGTTGTCTGACCGAGATGTTAACTCTGATGAGGAAGTCGCGTTTTTGAAGATGATCATTGAGAAGCAACTGTTTGCACACGCTGGTCTAGCAAAAGAGTATGCTTATAACAAAAAGGTTGAGGGTCTTTACCGACCGGGTTATTATGAAGCTTTGGGAAATGTCATATTGAAGAGGTTTCTGTTGCTTGTTATTATCCTGGATAGAGCTAAATCTCAGAGCTGTCTTTCTCTTAAATATGGTCTTGATGGAGTGGATGGGGGTTCTCCTTTATTGTTTACGGTACAGTCTAGTATCAAATCAAGTCAACAGGTGATCCATGATTTTCTCTCATCTGATATCATGCTTGGAGAAGGTAACGTCTCAGCGCATCTGGTGATTCTCGGATATAAAGTATCTCATCAGCAGTCTCCGATCGTGGATTTTGACTTTCGAGTTACAGATTTGTTTGCTGATCTTCAAGATGGGTTGCACCTCTGTAGAGCTATTCAACTTTTGCAGGATGACTCCTCTATCTTAATGAAAATGGTTGTTCCATCAGATACACATAAAAAGCACCTGTCAAATTGTGGCACTGCCCTGCAGTATCTTAAGGAGGCTGGTGTTGTTTTACACGATGATGGATTGATGATTGTGGAAGATGATATTGCTAATGGGGACAAGGAACTTACCATTTCCTTGCTTTGGAATATGTTTGTTCATTTGCAGCTACCTCTTTTGGTCAAGAAAACAACATTAGCTGATGAAATATGCAAAATCCGGGGAACTATGGATAGCTTTATGGATGTTGATTCTGCTCCACTGGAAATGCTTTTAAGTTGGATTCAGGCCATTTGTGAAATTTATGATTGCAAGATCGACAGTTTTTCTTCATTGGTAGATGGAAAAGCCGTATGGTGCTTGCTGGATTATCACTTCCGTAAGCAACTTTGTTGTGCCTGGTCATCGAAGGATCCTAATAAATCAAGTCAGGAAGAATCAATCATGTTAGCCAGTGAATATTCAGATGCCGTGCACAATTTCTTATTATCACAGAAATTGATGTCGCTATTGGGAAACTTTCCAGAGGTTCTGCAAATAAGTGACATTCTTGAGTATAATGGCGTCCCTAATGATCGTAGTGTGGTAATCCTGTTGGCATTCCTTTCATCTCAACTGATTGGGAAGAAAAACATGGATCAGATAAACTTTCATAAACTATTAGGCTGTGATTGTCAAAGTTCAGAGCGAATATGCTCAGTAAGACCTGAACCAACACAGATACAAGAAGAAACATATGTTCAGCATACTGAAGGTTCTGTAAGAAACTTTAAGGCTATCCAGGCTTGGTGGCAAGATATGGCTGAAAAGAACCGTAAACTTCCAAAACCATCAGCTCCTACTTTACAAAACTTCTCTACAAACAAAGATGAAATTAACATTTCAAGAGTAAATGCTGCAAAAAGGATACGGTCAGTGTTACTAATCCAACAAGCTGTAAGAAATTGGATGATGCGAAGACGTCAGGATAGAAGTATGCTGACTCATGATGCACACAGACATCAGGATAGAAGTATGCTGACTCATGATGCACACATTCATGATCTAGTAAATGCTGCTATTGTTGTCCAAAGATATACTCGTGGGTGGCTTGCAAGATCAAGATGCATTCATGGAGGCCCTCTGATTCATAAATCGTTAAGTATGTTTCAGGAAAATGGTGCAAAAAGGATACCGTCAGTATTACTTATCCAACAAGCTGCAAGAAATTGGATCATGCGAAGACATCAGGATAGAAGTATGATAACTCATGATGCACGTATCCACGATCAAGTAAATGCAGCTATTGTTGTTCAAAGATATACTCGTGGGTGGCTTGGAAGATCAAGATGTAATCTAGGGGGCCCTCTCATTCATAAATCCTCAAGTATGTTTCAGGAAAATGGTGCACTAGATGTTCGAACATGGGCAGCAGTTAAAATCCAACTTGCTTGGAATTATTATGTCTGTCACACTCTTCACAAAAAGCACTTTGCTGCTATTAAAATACAAAGTCATTTTTGTAGTTGGCTATTGAGAAGGAGGTTTCACATTCAAAGGCAAGCAACAATAAAAGTCCAAAGTGCTTTGCGTATGTTAAAATGTTGGAAGGCTTATCAGCAATACAAAGTTGCAACTAAATCAGCCACTACCATTCAATCTTATGTACGTGGATGGGCTGCCCGAAGAGAAGCTAACATACGTAGGCATCTCATCGTTACTATCCAAAGATACTGCCATGGTTGGTTGAGAAGGAGGCATTTCTTACATCAAAGAAAAGCTGTAGTGAAGATCCAAAGCACTATTCGGTGCCTGCTATACCGTCAGGCATTCCAATGTCTTAGACATGCTGCAGTGGAAATTCAACGGATAGTCAGGGGGGAAATTTGTCGAAGTAATCTCTTAGGTTCTTCGTGTCTACACCCTGTCATCCCCCACGGTTGTCTTTCAAAGACCACGAGTGCCTTTTATAGTAGTTTCGAACTAAATATAGTGTTTTGCTCTGTGTTGAAATTGCAAAGGTGGTGGAGGAGTGTCCTATCTTTAAAACTAAGAACAAAGTCTGCAGTACTTATTCAGTCTCGTATTCGAGAATGGTTAGCCAGGCAAAAGGCTTCTAGAGAGAAGCATTGTAGTGTTGTGATACAATCACACTGGAGAGGTTACCAGGCACGAAAAAAAGAGTCAAGGGAACAGCTCAAGGATATACGCTTGAGAGTGCAGAAGTCTTCTACTAGTGTGGACGATAGCATGCGTATTATCAACAGACTTGTTGCTGCACTTTCAGAACTACTAAGAATGAAAAGTCTCAGCAACATTCTTCATACTTGTGCCACTTTGGATATGGCTACAAGGTATTCTCAGAAATGTTGTGAAAGACTCGTGGATGCTGGAGCTATCAAGACCTTGCTGAAGCTTATTCACTCAGGCAGCCGAAGCATACCTGATCAGGAGGTTCTAAAGCATGCACTGTCAACTCTAAGAAACCTTGCTCGCTATCCACACTTGGTTGAAGTACTAACTGACTGTGAGGGGTCTGTAGAAACTATTCTTTGGGAGTTGCTAAGGAACAAGGAGGAAGGTTACTTCATTGCATCTGAACTTTTGAAGAAGATATGTTCTAGTCCTAAAGGTGCTGAAGCTGTACGCAAGTCACCTGCCCATTTAAAGAGACTGCGCAGTCTTGTGGAGGAACTAAGTAGGAAATCATGCAATGAGAAGAGGAATGCTCGACTCGCAAATGCTCGAGAGAATACAGAGAGGAGACTGAAGGAGGCTACTGTGATTCTGAAAATGGCAACAGAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm CH IQ

Protein Analysis

1383

Amino Acids

158.13

Weight (kDa)

9.71

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 418 - 507 6.8e-07 Calponin homology (CH) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 768, 927
AarI CACCTGC 1 cut(s) 4003
AasI GACNNNNNNGTC 2 cut(s) 181, 4017
AatII GACGTC 1 cut(s) 2323
Acc16I TGCGCA 1 cut(s) 4016
Acc36I ACCTGC 1 cut(s) 4003
AccB1I GGYRCC 1 cut(s) 3147
AccI GTMKAC 2 cut(s) 456, 3256
AccII CGCG 3 cut(s) 274, 461, 860
AccIII TCCGGA 2 cut(s) 288, 411
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 4 cut(s) 122, 1659, 3198, 3222
AcuI CTGAAG 5 cut(s) 326, 2130, 3770, 3996, 4127
AcyI GRCGYC 4 cut(s) 6, 441, 1923, 2320
AfaI GTAC 7 cut(s) 548, 797, 1104, 3006, 3408, 3855, 3984
AfiI CCNNNNNNNGG 2 cut(s) 451, 1503
AflII CTTAAG 1 cut(s) 1409
AhlI ACTAGT 1 cut(s) 3572
AjnI CCWGG 8 cut(s) 444, 624, 636, 1017, 1757, 2139, 3440, 3504
AjuI GAANNNNNNNTTGG 2 cut(s) 3126, 3158
Alw21I GWGCWC 3 cut(s) 255, 1836, 2749
Alw26I GTCTC 9 cut(s) 306, 315, 483, 1186, 1233, 3423, 3650, 4003, 4096
Alw44I GTGCAC 2 cut(s) 1832, 2745
AlwNI CAGNNNCTG 1 cut(s) 304
Ama87I CYCGRG 3 cut(s) 254, 314, 4083
Aor13HI TCCGGA 2 cut(s) 288, 411
AoxI GGCC 6 cut(s) 243, 400, 744, 1647, 2476, 2701
ApaI GGGCCC 1 cut(s) 2705
ApaLI GTGCAC 2 cut(s) 1832, 2745
ApoI RAATTY 4 cut(s) 122, 1659, 3198, 3222
Asp700I GAANNNNTTC 1 cut(s) 3885
AspLEI GCGC 3 cut(s) 276, 1189, 4017
AspS9I GGNCC 5 cut(s) 280, 448, 2477, 2701, 2702
AsuC2I CCSGG 4 cut(s) 172, 452, 957, 1580
AsuHPI GGTGA 8 cut(s) 18, 24, 142, 319, 518, 1144, 1207, 3984
AsuII TTCGAA 4 cut(s) 351, 784, 2758, 3318
AvaI CYCGRG 3 cut(s) 254, 314, 4083
AvaII GGWCC 2 cut(s) 280, 448
BaeGI GKGCMC 3 cut(s) 1836, 2705, 2749
BamHI GGATCC 2 cut(s) 472, 1771
BanI GGYRCC 1 cut(s) 3147
BanII GRGCYC 2 cut(s) 255, 2705
BarI GAAGNNNNNNTAC 2 cut(s) 3021, 3053
BauI CACGAG 4 cut(s) 2439, 2664, 3295, 3719
BbsI GAAGAC 3 cut(s) 2323, 2587, 3558
Bbv12I GWGCWC 3 cut(s) 255, 1836, 2749
BccI CCATC 8 cut(s) 1061, 1070, 1277, 1363, 1433, 1694, 2197, 3006
BceAI ACGGC 8 cut(s) 7, 25, 190, 212, 230, 415, 1694, 1814
BciT130I CCWGG 8 cut(s) 446, 626, 638, 1019, 1759, 2141, 3442, 3506
BciVI GTATCC 3 cut(s) 2259, 2523, 2630
BclI TGATCA 2 cut(s) 873, 3784
BcnI CCSGG 4 cut(s) 172, 452, 957, 1580
BcoDI GTCTC 9 cut(s) 306, 315, 483, 1186, 1233, 3423, 3650, 4003, 4096
BcuI ACTAGT 1 cut(s) 3572
BfmI CTRYAG 6 cut(s) 1297, 1400, 3120, 3189, 3402, 3876
BfrI CTTAAG 1 cut(s) 1409
BfuAI ACCTGC 1 cut(s) 4003
BfuI GTATCC 3 cut(s) 2259, 2523, 2630
BmcAI AGTACT 3 cut(s) 548, 3408, 3855
Bme18I GGWCC 2 cut(s) 280, 448
BmeT110I CYCGRG 3 cut(s) 254, 314, 4083
BmgT120I GGNCC 5 cut(s) 280, 448, 2477, 2701, 2702
BmiI GGNNCC 6 cut(s) 436, 474, 1773, 2702, 2703, 3149
BpiI GAAGAC 3 cut(s) 2323, 2587, 3558
BplI GAGNNNNNCTC 2 cut(s) 4051, 4083
BpmI CTGGAG 2 cut(s) 3514, 3750
Bpu10I CCTNAGC 1 cut(s) 3902
Bpu14I TTCGAA 4 cut(s) 351, 784, 2758, 3318
BpuEI CTTGAG 5 cut(s) 224, 1931, 2707, 3521, 3571
BpuMI CCSGG 4 cut(s) 172, 452, 957, 1580
BsaAI YACGTR 3 cut(s) 2618, 3008, 3041
BsaBI GATNNNNATC 1 cut(s) 393
BsaHI GRCGYC 4 cut(s) 6, 441, 1923, 2320
BsaJI CCNNGG 6 cut(s) 170, 246, 625, 1579, 3076, 3274
BsaWI WCCGGW 3 cut(s) 288, 382, 411
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 1503
Bse1I ACTGG 3 cut(s) 1626, 1812, 3497
Bse3DI GCAATG 3 cut(s) 706, 3927, 4057
Bse8I GATNNNNATC 1 cut(s) 393
BseAI TCCGGA 2 cut(s) 288, 411
BseBI CCWGG 8 cut(s) 446, 626, 638, 1019, 1759, 2141, 3442, 3506
BseDI CCNNGG 6 cut(s) 170, 246, 625, 1579, 3076, 3274
BseGI GGATG 7 cut(s) 1081, 1327, 1609, 2313, 3017, 3267, 3730
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 451, 1503
BseMI GCAATG 3 cut(s) 706, 3927, 4057
BseMII CTCAG 7 cut(s) 725, 1049, 1197, 2070, 3661, 3713, 3777
BseNI ACTGG 3 cut(s) 1626, 1812, 3497
BseRI GAGGAG 3 cut(s) 1318, 3382, 4114
BseSI GKGCMC 3 cut(s) 1836, 2705, 2749
BsgI GTGCAG 2 cut(s) 3578, 3600
Bsh1236I CGCG 3 cut(s) 274, 461, 860
Bsh1285I CGRYCG 3 cut(s) 387, 956, 1236
BshFI GGCC 6 cut(s) 245, 402, 746, 1649, 2478, 2703
BshNI GGYRCC 1 cut(s) 3147
BsiEI CGRYCG 3 cut(s) 387, 956, 1236
BsiHKAI GWGCWC 3 cut(s) 255, 1836, 2749
BsiHKCI CYCGRG 3 cut(s) 254, 314, 4083
BsiSI CCGG 8 cut(s) 172, 289, 383, 412, 438, 451, 956, 1579
BslFI GGGAC 3 cut(s) 440, 1490, 1910
BslI CCNNNNNNNGG 2 cut(s) 451, 1503
BsmAI GTCTC 9 cut(s) 306, 315, 483, 1186, 1233, 3423, 3650, 4003, 4096
BsmBI CGTCTC 1 cut(s) 1186
BsmFI GGGAC 3 cut(s) 440, 1490, 1910
BsmI GAATGC 5 cut(s) 612, 1958, 2466, 3167, 4069
BsnI GGCC 6 cut(s) 245, 402, 746, 1649, 2478, 2703
BsoBI CYCGRG 3 cut(s) 254, 314, 4083
Bsp119I TTCGAA 4 cut(s) 351, 784, 2758, 3318
Bsp120I GGGCCC 1 cut(s) 2701
Bsp1286I GDGCHC 4 cut(s) 255, 1836, 2705, 2749
Bsp13I TCCGGA 2 cut(s) 288, 411
Bsp1407I TGTACA 1 cut(s) 795
Bsp19I CCATGG 1 cut(s) 3076
BspANI GGCC 6 cut(s) 245, 402, 746, 1649, 2478, 2703
BspCNI CTCAG 7 cut(s) 726, 1048, 1196, 2069, 3660, 3712, 3776
BspEI TCCGGA 2 cut(s) 288, 411
BspFNI CGCG 3 cut(s) 274, 461, 860
BspHI TCATGA 4 cut(s) 2344, 2383, 2398, 2608
BspLI GGNNCC 6 cut(s) 436, 474, 1773, 2702, 2703, 3149
BspMAI CTGCAG 3 cut(s) 1404, 3193, 3406
BspMI ACCTGC 1 cut(s) 4003
BspT104I TTCGAA 4 cut(s) 351, 784, 2758, 3318
BspT107I GGYRCC 1 cut(s) 3147
BspTI CTTAAG 1 cut(s) 1409
BsrDI GCAATG 3 cut(s) 706, 3927, 4057
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 3 cut(s) 1626, 1812, 3497
BssECI CCNNGG 6 cut(s) 170, 246, 625, 1579, 3076, 3274
BssNI GRCGYC 4 cut(s) 6, 441, 1923, 2320
BssSI CACGAG 4 cut(s) 2439, 2664, 3295, 3719
BssT1I CCWWGG 1 cut(s) 3076
Bst2BI CACGAG 4 cut(s) 2439, 2664, 3295, 3719
Bst2UI CCWGG 8 cut(s) 446, 626, 638, 1019, 1759, 2141, 3442, 3506
Bst6I CTCTTC 7 cut(s) 74, 353, 416, 986, 2820, 3019, 4052
BstACI GRCGYC 4 cut(s) 6, 441, 1923, 2320
BstAFI CTTAAG 1 cut(s) 1409
BstAUI TGTACA 1 cut(s) 795
BstBAI YACGTR 3 cut(s) 2618, 3008, 3041
BstBI TTCGAA 4 cut(s) 351, 784, 2758, 3318
BstC8I GCNNGC 8 cut(s) 217, 1721, 2451, 2901, 3152, 3590, 3804, 3832
BstDSI CCRYGG 3 cut(s) 246, 3076, 3274
BstEII GGTNACC 1 cut(s) 3500
BstF5I GGATG 7 cut(s) 1081, 1327, 1609, 2313, 3017, 3267, 3730
BstFNI CGCG 3 cut(s) 274, 461, 860
BstHHI GCGC 3 cut(s) 276, 1189, 4017
BstMAI GTCTC 9 cut(s) 306, 315, 483, 1186, 1233, 3423, 3650, 4003, 4096
BstMCI CGRYCG 3 cut(s) 387, 956, 1236
BstMWI GCNNNNNNNGC 5 cut(s) 438, 908, 2141, 3774, 4073
BstNI CCWGG 8 cut(s) 446, 626, 638, 1019, 1759, 2141, 3442, 3506
BstNSI RCATGY 3 cut(s) 3188, 3592, 3806
BstPI GGTNACC 1 cut(s) 3500
BstSFI CTRYAG 6 cut(s) 1297, 1400, 3120, 3189, 3402, 3876
BstSLI GKGCMC 3 cut(s) 1836, 2705, 2749
BstSNI TACGTA 1 cut(s) 3041
BstUI CGCG 3 cut(s) 274, 461, 860
BstV2I GAAGAC 3 cut(s) 2323, 2587, 3558
BstX2I RGATCY 4 cut(s) 362, 472, 1771, 3129
BstYI RGATCY 4 cut(s) 362, 472, 1771, 3129
BsuI GTATCC 3 cut(s) 2259, 2523, 2630
BsuRI GGCC 6 cut(s) 245, 402, 746, 1649, 2478, 2703
BtgI CCRYGG 3 cut(s) 246, 3076, 3274
BtsCI GGATG 7 cut(s) 1081, 1327, 1609, 2313, 3017, 3267, 3730
BtsI GCAGTG 2 cut(s) 1392, 3198
BtsIMutI CAGTG 7 cut(s) 1392, 1619, 1819, 2280, 3198, 3490, 3806
BveI ACCTGC 1 cut(s) 4003
Cac8I GCNNGC 8 cut(s) 217, 1721, 2451, 2901, 3152, 3590, 3804, 3832
CaiI CAGNNNCTG 1 cut(s) 304
CciI TCATGA 4 cut(s) 2344, 2383, 2398, 2608
CfoI GCGC 3 cut(s) 276, 1189, 4017
Cfr13I GGNCC 5 cut(s) 280, 448, 2477, 2701, 2702
CseI GACGC 4 cut(s) 14, 430, 467, 1912
Csp6I GTAC 7 cut(s) 547, 796, 1103, 3005, 3407, 3854, 3983
CspCI CAANNNNNGTGG 4 cut(s) 116, 151, 3344, 3379
CviQI GTAC 7 cut(s) 547, 796, 1103, 3005, 3407, 3854, 3983
DraI TTTAAA 2 cut(s) 3384, 4005
DrdI GACNNNNNNGTC 2 cut(s) 181, 4017
DseDI GACNNNNNNGTC 2 cut(s) 181, 4017
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 7 cut(s) 74, 353, 416, 986, 2820, 3019, 4052
EarI CTCTTC 7 cut(s) 74, 353, 416, 986, 2820, 3019, 4052
EciI GGCGGA 2 cut(s) 192, 202
Ecl136II GAGCTC 1 cut(s) 253
Eco105I TACGTA 1 cut(s) 3041
Eco130I CCWWGG 1 cut(s) 3076
Eco24I GRGCYC 2 cut(s) 255, 2705
Eco32I GATATC 1 cut(s) 1159
Eco47I GGWCC 2 cut(s) 280, 448
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 5 cut(s) 326, 2130, 3770, 3996, 4127
Eco88I CYCGRG 3 cut(s) 254, 314, 4083
Eco91I GGTNACC 1 cut(s) 3500
EcoICRI GAGCTC 1 cut(s) 253
EcoO109I RGGNCCY 3 cut(s) 2477, 2701, 2702
EcoO65I GGTNACC 1 cut(s) 3500
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 8 cut(s) 444, 624, 636, 1017, 1757, 2139, 3440, 3504
EcoRV GATATC 1 cut(s) 1159
EcoT14I CCWWGG 1 cut(s) 3076
EcoT22I ATGCAT 1 cut(s) 2468
EcoT38I GRGCYC 2 cut(s) 255, 2705
ErhI CCWWGG 1 cut(s) 3076
Esp3I CGTCTC 1 cut(s) 1186
FaqI GGGAC 3 cut(s) 440, 1490, 1910
FauI CCCGC 1 cut(s) 224
FauNDI CATATG 1 cut(s) 2095
FbaI TGATCA 2 cut(s) 873, 3784
FblI GTMKAC 2 cut(s) 456, 3256
FokI GGATG 7 cut(s) 1088, 1334, 1616, 2320, 3024, 3254, 3737
FriOI GRGCYC 2 cut(s) 255, 2705
FspI TGCGCA 1 cut(s) 4016
GlaI GCGC 3 cut(s) 275, 1188, 4016
GsuI CTGGAG 2 cut(s) 3514, 3750
HaeIII GGCC 6 cut(s) 245, 402, 746, 1649, 2478, 2703
HapII CCGG 8 cut(s) 172, 289, 383, 412, 438, 451, 956, 1579
HgaI GACGC 4 cut(s) 14, 430, 467, 1912
HhaI GCGC 3 cut(s) 276, 1189, 4017
Hin1I GRCGYC 4 cut(s) 6, 441, 1923, 2320
Hin6I GCGC 3 cut(s) 274, 1187, 4015
HinP1I GCGC 3 cut(s) 274, 1187, 4015
HincII GTYRAC 4 cut(s) 457, 841, 1127, 3813
HindII GTYRAC 4 cut(s) 457, 841, 1127, 3813
HindIII AAGCTT 3 cut(s) 572, 969, 3752
HpaI GTTAAC 1 cut(s) 841
HpaII CCGG 8 cut(s) 172, 289, 383, 412, 438, 451, 956, 1579
HphI GGTGA 8 cut(s) 18, 24, 142, 319, 518, 1144, 1207, 3984
Hpy99I CGWCG 2 cut(s) 40, 461
HpyCH4IV ACGT 6 cut(s) 46, 1179, 2320, 2617, 3007, 3040
HpyF10VI GCNNNNNNNGC 5 cut(s) 438, 908, 2141, 3774, 4073
HpySE526I ACGT 6 cut(s) 46, 1179, 2320, 2617, 3007, 3040
Hsp92I GRCGYC 4 cut(s) 6, 441, 1923, 2320
HspAI GCGC 3 cut(s) 274, 1187, 4015
Kpn2I TCCGGA 2 cut(s) 288, 411
Ksp22I TGATCA 2 cut(s) 873, 3784
KspAI GTTAAC 1 cut(s) 841
LmnI GCTCC 7 cut(s) 250, 295, 414, 440, 1621, 2200, 3731
MaeII ACGT 6 cut(s) 46, 1179, 2320, 2617, 3007, 3040
MflI RGATCY 4 cut(s) 362, 472, 1771, 3129
MhlI GDGCHC 4 cut(s) 255, 1836, 2705, 2749
MlyI GAGTC 8 cut(s) 485, 644, 1319, 2335, 2374, 3530, 3711, 4065
MmeI TCCRAC 6 cut(s) 709, 1619, 2311, 2575, 2806, 2924
Mph1103I ATGCAT 1 cut(s) 2468
MroI TCCGGA 2 cut(s) 288, 411
MroXI GAANNNNTTC 1 cut(s) 3885
MslI CAYNNNNRTG 5 cut(s) 1437, 2397, 3172, 3471, 3666
MspA1I CMGCKG 1 cut(s) 432
MspCI CTTAAG 1 cut(s) 1409
MspI CCGG 8 cut(s) 172, 289, 383, 412, 438, 451, 956, 1579
Mva1269I GAATGC 5 cut(s) 612, 1958, 2466, 3167, 4069
MvaI CCWGG 8 cut(s) 446, 626, 638, 1019, 1759, 2141, 3442, 3506
MvnI CGCG 3 cut(s) 274, 461, 860
MwoI GCNNNNNNNGC 5 cut(s) 438, 908, 2141, 3774, 4073
NciI CCSGG 4 cut(s) 172, 452, 957, 1580
NcoI CCATGG 1 cut(s) 3076
NdeI CATATG 1 cut(s) 2095
NlaIV GGNNCC 6 cut(s) 436, 474, 1773, 2702, 2703, 3149
NmuCI GTSAC 8 cut(s) 24, 30, 42, 261, 718, 1901, 2807, 3990
NsbI TGCGCA 1 cut(s) 4016
NsiI ATGCAT 1 cut(s) 2468
NspI RCATGY 3 cut(s) 3188, 3592, 3806
NspV TTCGAA 4 cut(s) 351, 784, 2758, 3318
PaeI GCATGC 2 cut(s) 3592, 3806
PaeR7I CTCGAG 3 cut(s) 254, 314, 4083
PagI TCATGA 4 cut(s) 2344, 2383, 2398, 2608
PaqCI CACCTGC 1 cut(s) 4003
PcsI WCGNNNNNNNCGW 1 cut(s) 109
PctI GAATGC 5 cut(s) 612, 1958, 2466, 3167, 4069
PdmI GAANNNNTTC 1 cut(s) 3885
PfoI TCCNGGA 1 cut(s) 1017
Ple19I CGATCG 1 cut(s) 1236
PleI GAGTC 8 cut(s) 485, 644, 1319, 2335, 2374, 3529, 3711, 4065
PpsI GAGTC 8 cut(s) 485, 644, 1319, 2335, 2374, 3529, 3711, 4065
Ppu21I YACGTR 3 cut(s) 2618, 3008, 3041
PsiI TTATAA 2 cut(s) 768, 927
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 8 cut(s) 444, 624, 636, 1017, 1757, 2139, 3440, 3504
PspEI GGTNACC 1 cut(s) 3500
PspGI CCWGG 8 cut(s) 444, 624, 636, 1017, 1757, 2139, 3440, 3504
PspN4I GGNNCC 6 cut(s) 436, 474, 1773, 2702, 2703, 3149
PspOMI GGGCCC 1 cut(s) 2701
PspPI GGNCC 5 cut(s) 280, 448, 2477, 2701, 2702
PspXI VCTCGAGB 1 cut(s) 254
PstI CTGCAG 3 cut(s) 1404, 3193, 3406
PstNI CAGNNNCTG 1 cut(s) 304
PsuI RGATCY 4 cut(s) 362, 472, 1771, 3129
PvuI CGATCG 1 cut(s) 1236
RsaI GTAC 7 cut(s) 548, 797, 1104, 3006, 3408, 3855, 3984
RsaNI GTAC 7 cut(s) 547, 796, 1103, 3005, 3407, 3854, 3983
RseI CAYNNNNRTG 5 cut(s) 1437, 2397, 3172, 3471, 3666
SacI GAGCTC 1 cut(s) 255
SalI GTCGAC 1 cut(s) 455
Sau96I GGNCC 5 cut(s) 280, 448, 2477, 2701, 2702
ScaI AGTACT 3 cut(s) 548, 3408, 3855
SchI GAGTC 8 cut(s) 485, 644, 1319, 2335, 2374, 3530, 3711, 4065
SduI GDGCHC 4 cut(s) 255, 1836, 2705, 2749
SfcI CTRYAG 6 cut(s) 1297, 1400, 3120, 3189, 3402, 3876
Sfr274I CTCGAG 3 cut(s) 254, 314, 4083
SfuI TTCGAA 4 cut(s) 351, 784, 2758, 3318
SinI GGWCC 2 cut(s) 280, 448
SlaI CTCGAG 3 cut(s) 254, 314, 4083
SmiMI CAYNNNNRTG 5 cut(s) 1437, 2397, 3172, 3471, 3666
SmlI CTYRAG 9 cut(s) 239, 254, 314, 1409, 1910, 2722, 3536, 3550, 4083
SmoI CTYRAG 9 cut(s) 239, 254, 314, 1409, 1910, 2722, 3536, 3550, 4083
SnaBI TACGTA 1 cut(s) 3041
SpeI ACTAGT 1 cut(s) 3572
SphI GCATGC 2 cut(s) 3592, 3806
SspI AATATT 2 cut(s) 676, 1820
SstI GAGCTC 1 cut(s) 255
StyI CCWWGG 1 cut(s) 3076
TaiI ACGT 6 cut(s) 49, 1182, 2323, 2620, 3010, 3043
TaqII GACCGA 2 cut(s) 737, 846
TatI WGTACW 4 cut(s) 546, 795, 3406, 3853
TauI GCSGC 9 cut(s) 11, 22, 158, 195, 198, 372, 435, 464, 484
TscAI CASTG 7 cut(s) 1399, 1626, 1819, 2280, 3198, 3497, 3813
TseFI GTSAC 8 cut(s) 24, 30, 42, 261, 718, 1901, 2807, 3990
Tsp45I GTSAC 8 cut(s) 24, 30, 42, 261, 718, 1901, 2807, 3990
TspGWI ACGGA 2 cut(s) 1727, 3220
TspRI CASTG 7 cut(s) 1399, 1626, 1819, 2280, 3198, 3497, 3813
Vha464I CTTAAG 1 cut(s) 1409
VneI GTGCAC 2 cut(s) 1832, 2745
VpaK11BI GGWCC 2 cut(s) 280, 448
XapI RAATTY 4 cut(s) 122, 1659, 3198, 3222
XbaI TCTAGA 1 cut(s) 3455
XceI RCATGY 3 cut(s) 3188, 3592, 3806
XcmI CCANNNNNNNNNTGG 1 cut(s) 3679
XhoI CTCGAG 3 cut(s) 254, 314, 4083
XmiI GTMKAC 2 cut(s) 456, 3256
XmnI GAANNNNTTC 1 cut(s) 3885
ZraI GACGTC 1 cut(s) 2321
ZrmI AGTACT 3 cut(s) 548, 3408, 3855
Zsp2I ATGCAT 1 cut(s) 2468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.