AT4G22330

Alkaline ceramidase 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008451
Physical Location & Seq
Forward (+)
11797548 .. 11799946
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22330.1

Sequence Viewer

Length: 768 bp
ATGGCAGATGGGATATCGAGCTTTTGGGGTCCTGTGACTTCTACTATAGAGTGTTGTGAGATGAACTACGCCTACTCATCCTACATTGCTGAGTTCTACAACACTATATCCAATGTCCCTGGAATCCTATTGGCTCTCATTGGTCTTGTCAATGCATTAAGGCAACGGTTTGAGAAGAGGTTTAGCATTCTTCACATTTCGAATATGATACTTGCTATCGGCAGCATGCTCTACCATGCCACTTTGCAACACGTGCAACAACAGAGTGATGAGACCCCAATGGTGTGGGAGATACTTCTATACATGTACATCCTTTACTCACCAGATTGGCATTACCGAAGTACAATGCCCACTTTTCTCTTCCTCTACGGTGCTGCCTTTGCCATAGTCCATGCTTACCTCAGGTTTGGAATTGGTTTCAAGGTCCACTACGTGATACTTTGCCTTCTATGCATCCCTCGGATGTACAAGTACTACATTCACACAGAGGACACAGCAGCCAAAAGGATTGCGAAATGGTATGTTGCTACGATCTTAGTGGGAAGCATATGCTGGTTCTGTGACCGTGTCTTCTGCAAGACGATATCTCAGTGGCCTGTGAATCCTCAGGGACATGCTCTGTGGCATGTGTTCATGAGTTTCAACTCTTACTGCGCAAACACATTCTTGATGTTCTGCAGAGCTCAGCAACGTGGATGGAACCCAAAGGTAAAATACTTTCTGGGAGTTCTCCCTTATGTCAAGATCGAGAAGCCAAAAACACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

255

Amino Acids

29.74

Weight (kDa)

8.98

Isoelectric Point (pI)

55.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 10 - 247 1.6e-63 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 655
AcvI CACGTG 1 cut(s) 253
AdeI CACNNNGTG 1 cut(s) 433
AfaI GTAC 4 cut(s) 308, 343, 467, 473
AflIII ACRYGT 2 cut(s) 250, 303
AgsI TTSAA 2 cut(s) 421, 643
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 2 cut(s) 21, 683
AluI AGCT 2 cut(s) 21, 683
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 1 cut(s) 266
AoxI GGCC 1 cut(s) 593
ApeKI GCWGC 3 cut(s) 222, 374, 497
AspLEI GCGC 1 cut(s) 656
AspS9I GGNCC 2 cut(s) 29, 424
AsuHPI GGTGA 1 cut(s) 312
AsuII TTCGAA 1 cut(s) 200
AvaII GGWCC 2 cut(s) 29, 424
AxyI CCTNAGG 2 cut(s) 401, 606
BanII GRGCYC 1 cut(s) 685
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 3 cut(s) 234, 361, 509
BccI CCATC 2 cut(s) 2, 690
BciT130I CCWGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 266
BfmI CTRYAG 2 cut(s) 45, 676
BisI GCNGC 3 cut(s) 223, 375, 498
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 3 cut(s) 224, 376, 499
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 2 cut(s) 29, 424
BmgT120I GGNCC 2 cut(s) 29, 424
BmiI GGNNCC 2 cut(s) 30, 701
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 562
Bpu1102I GCTNAGC 1 cut(s) 684
Bpu14I TTCGAA 1 cut(s) 200
BsaAI YACGTR 2 cut(s) 253, 433
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 2 cut(s) 118, 458
Bse21I CCTNAGG 2 cut(s) 401, 606
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 118, 458
BseGI GGATG 5 cut(s) 77, 309, 453, 468, 701
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 5 cut(s) 81, 415, 602, 620, 698
BseXI GCAGC 3 cut(s) 234, 361, 509
BshFI GGCC 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 685
BslFI GGGAC 2 cut(s) 101, 624
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 2 cut(s) 101, 624
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 1 cut(s) 595
Bso31I GGTCTC 1 cut(s) 266
Bsp119I TTCGAA 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 685
Bsp1407I TGTACA 2 cut(s) 306, 465
Bsp143I GATC 2 cut(s) 531, 744
Bsp1720I GCTNAGC 1 cut(s) 684
BspANI GGCC 1 cut(s) 595
BspCNI CTCAG 5 cut(s) 82, 414, 601, 619, 697
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 2 cut(s) 30, 701
BspMAI CTGCAG 1 cut(s) 680
BspT104I TTCGAA 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 84
BsrGI TGTACA 2 cut(s) 306, 465
BssECI CCNNGG 2 cut(s) 118, 458
BssMI GATC 2 cut(s) 531, 744
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 168, 371, 566
Bst6I CTCTTC 2 cut(s) 170, 365
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 2 cut(s) 306, 465
BstBAI YACGTR 2 cut(s) 253, 433
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 6 cut(s) 90, 401, 535, 588, 606, 684
BstF5I GGATG 5 cut(s) 77, 309, 453, 468, 701
BstHHI GCGC 1 cut(s) 656
BstKTI GATC 2 cut(s) 534, 747
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 2 cut(s) 531, 744
BstMWI GCNNNNNNNGC 3 cut(s) 253, 380, 450
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 4 cut(s) 229, 307, 617, 629
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 2 cut(s) 45, 676
BstV1I GCAGC 3 cut(s) 234, 361, 509
BstV2I GAAGAC 1 cut(s) 562
BstXI CCANNNNNNTGG 1 cut(s) 285
Bsu36I CCTNAGG 2 cut(s) 401, 606
BsuRI GGCC 1 cut(s) 595
BtsCI GGATG 5 cut(s) 77, 309, 453, 468, 701
BtsIMutI CAGTG 1 cut(s) 596
Cac8I GCNNGC 1 cut(s) 227
CciI TCATGA 1 cut(s) 633
CfoI GCGC 1 cut(s) 656
Cfr13I GGNCC 2 cut(s) 29, 424
Csp6I GTAC 4 cut(s) 307, 342, 466, 472
CviAII CATG 7 cut(s) 226, 236, 304, 392, 614, 626, 634
CviJI RGCY 6 cut(s) 21, 134, 500, 595, 683, 754
CviKI_1 RGCY 6 cut(s) 21, 134, 500, 595, 683, 754
CviQI GTAC 4 cut(s) 307, 342, 466, 472
DdeI CTNAG 6 cut(s) 90, 401, 535, 588, 606, 684
DpnI GATC 2 cut(s) 533, 746
DpnII GATC 2 cut(s) 531, 744
DraIII CACNNNGTG 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 170, 365
EarI CTCTTC 2 cut(s) 170, 365
Ecl136II GAGCTC 1 cut(s) 683
Eco24I GRGCYC 1 cut(s) 685
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 2 cut(s) 15, 585
Eco47I GGWCC 2 cut(s) 29, 424
Eco53kI GAGCTC 1 cut(s) 683
Eco72I CACGTG 1 cut(s) 253
Eco81I CCTNAGG 2 cut(s) 401, 606
EcoICRI GAGCTC 1 cut(s) 683
EcoO109I RGGNCCY 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 118
EcoRV GATATC 2 cut(s) 15, 585
EcoT22I ATGCAT 2 cut(s) 157, 455
EcoT38I GRGCYC 1 cut(s) 685
FaeI CATG 7 cut(s) 229, 239, 307, 395, 617, 629, 637
FaqI GGGAC 2 cut(s) 101, 624
FatI CATG 7 cut(s) 225, 235, 303, 391, 613, 625, 633
FauNDI CATATG 1 cut(s) 548
Fnu4HI GCNGC 3 cut(s) 223, 375, 498
FokI GGATG 5 cut(s) 64, 296, 440, 475, 708
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 3 cut(s) 223, 375, 498
FspI TGCGCA 1 cut(s) 655
GlaI GCGC 1 cut(s) 655
GluI GCNGC 3 cut(s) 223, 375, 498
HaeIII GGCC 1 cut(s) 595
HhaI GCGC 1 cut(s) 656
Hin1II CATG 7 cut(s) 229, 239, 307, 395, 617, 629, 637
Hin6I GCGC 1 cut(s) 654
HinP1I GCGC 1 cut(s) 654
HinfI GANTC 2 cut(s) 123, 601
HphI GGTGA 1 cut(s) 312
Hpy166II GTNNAC 1 cut(s) 427
Hpy188I TCNGA 1 cut(s) 462
Hpy188III TCNNGA 4 cut(s) 634, 667, 742, 748
Hpy8I GTNNAC 1 cut(s) 427
HpyAV CCTTC 1 cut(s) 455
HpyCH4III ACNGT 3 cut(s) 168, 371, 566
HpyCH4IV ACGT 3 cut(s) 252, 432, 691
HpyCH4V TGCA 6 cut(s) 155, 247, 256, 453, 576, 678
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 380, 450
HpyF3I CTNAG 6 cut(s) 90, 401, 535, 588, 606, 684
HpySE526I ACGT 3 cut(s) 252, 432, 691
Hsp92II CATG 7 cut(s) 229, 239, 307, 395, 617, 629, 637
HspAI GCGC 1 cut(s) 654
Kzo9I GATC 2 cut(s) 531, 744
LpnPI CCDG 9 cut(s) 45, 105, 132, 336, 388, 538, 593, 609, 707
Lsp1109I GCAGC 3 cut(s) 234, 361, 509
LweI GCATC 1 cut(s) 462
MaeII ACGT 3 cut(s) 252, 432, 691
MaeIII GTNAC 2 cut(s) 34, 560
MalI GATC 2 cut(s) 533, 746
MboI GATC 2 cut(s) 531, 744
MboII GAAGA 4 cut(s) 182, 187, 352, 562
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 1 cut(s) 411
MnlI CCTC 6 cut(s) 171, 374, 410, 468, 481, 615
Mph1103I ATGCAT 2 cut(s) 157, 455
MseI TTAA 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 253, 380, 450
NdeI CATATG 1 cut(s) 548
NdeII GATC 2 cut(s) 531, 744
NlaIII CATG 7 cut(s) 229, 239, 307, 395, 617, 629, 637
NlaIV GGNNCC 2 cut(s) 30, 701
NmuCI GTSAC 2 cut(s) 34, 560
NsbI TGCGCA 1 cut(s) 655
NsiI ATGCAT 2 cut(s) 157, 455
NspI RCATGY 4 cut(s) 229, 307, 617, 629
NspV TTCGAA 1 cut(s) 200
PaeI GCATGC 1 cut(s) 229
PagI TCATGA 1 cut(s) 633
PciI ACATGT 1 cut(s) 303
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 123, 601
PflFI GACNNNGTC 1 cut(s) 566
PkrI GCNGC 3 cut(s) 224, 376, 499
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
Ppu21I YACGTR 2 cut(s) 253, 433
PpuMI RGGWCCY 1 cut(s) 29
PscI ACATGT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 685
Psp5II RGGWCCY 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 118
PspCI CACGTG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 30, 701
PspPI GGNCC 2 cut(s) 29, 424
PspPPI RGGWCCY 1 cut(s) 29
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 1 cut(s) 680
PsyI GACNNNGTC 1 cut(s) 566
RsaI GTAC 4 cut(s) 308, 343, 467, 473
RsaNI GTAC 4 cut(s) 307, 342, 466, 472
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 1 cut(s) 158
SatI GCNGC 3 cut(s) 223, 375, 498
Sau3AI GATC 2 cut(s) 531, 744
Sau96I GGNCC 2 cut(s) 29, 424
ScaI AGTACT 1 cut(s) 473
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 1 cut(s) 462
SfcI CTRYAG 2 cut(s) 45, 676
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 2 cut(s) 29, 424
SphI GCATGC 1 cut(s) 229
Sse9I AATT 1 cut(s) 411
SstI GAGCTC 1 cut(s) 685
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 168, 371, 566
TaiI ACGT 3 cut(s) 255, 435, 694
TaqI TCGA 3 cut(s) 17, 200, 747
TasI AATT 1 cut(s) 411
TatI WGTACW 4 cut(s) 306, 341, 465, 471
TfiI GAWTC 2 cut(s) 123, 601
Tru1I TTAA 1 cut(s) 158
Tru9I TTAA 1 cut(s) 158
TscAI CASTG 1 cut(s) 596
TseFI GTSAC 2 cut(s) 34, 560
TseI GCWGC 3 cut(s) 222, 374, 497
Tsp45I GTSAC 2 cut(s) 34, 560
TspDTI ATGAA 2 cut(s) 77, 622
TspRI CASTG 1 cut(s) 596
Tth111I GACNNNGTC 1 cut(s) 566
VpaK11BI GGWCC 2 cut(s) 29, 424
XceI RCATGY 4 cut(s) 229, 307, 617, 629
ZrmI AGTACT 1 cut(s) 473
Zsp2I ATGCAT 2 cut(s) 157, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.