Rh5DG013700

Alkaline ceramidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008451
Physical Location & Seq
Reverse (-)
977180 .. 979751
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG013700.1

Sequence Viewer

Length: 558 bp
ATGGAAAGCTTAATTTTGAAGATTACTTTTGGATATACTTGCAGGCAACAGCAGAGCGATGAGACTCCTATGGTTTGGGAGATGCTCCTATACATGTATATCCTCTACTCCCCAGATTGGCACTATCGCACTACAATGCCGACCTTCCTCTTCCTATATGGTGTTGTTTTTGCTGCAGTTCATTCAGTGGTCCGATTTGAGATTGGTTTTAAGGTGCACTATGTGATCTTGTGTCTTCTTTGCATACCTCGAATGTACAAGTATTATATATACACACAGGACGTCTATGCCAAGAGGCTCGCAAAGCTGTATGTGGTCACTCTTCTTATAGGGAGTTTGTGTTGGCTCGGCGACAATGTTTTCTGCAAAGAGATATCCAGTTGGCCCGTTAACCCACAAGGTCATGCCTTGTGGCATGTATTCATGGGTTTTAATTCCTATTTTGCAAACACTTTCTTGATGTTTTGCCGGGCTGAACAACGAGGATGGGCTCCTAAGATTGTTCGTTTTATGGGTCTCTTGCCCTATGTGAAGATTGAAAAACCAAAAACCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

185

Amino Acids

21.99

Weight (kDa)

9.08

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 15 - 169 6.1e-31 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 223
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 117
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 2 cut(s) 19, 539
AluBI AGCT 2 cut(s) 9, 307
AluI AGCT 2 cut(s) 9, 307
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 2 cut(s) 56, 521
Alw44I GTGCAC 1 cut(s) 215
AoxI GGCC 1 cut(s) 383
ApaLI GTGCAC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 173
AspS9I GGNCC 2 cut(s) 190, 384
AsuC2I CCSGG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 219
BanII GRGCYC 1 cut(s) 493
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 160
BccI CCATC 1 cut(s) 480
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 2 cut(s) 56, 521
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 470
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 2 cut(s) 190, 384
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 1 cut(s) 72
BpiI GAAGAC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 521
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse1I ACTGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 491
BseLI CCNNNNNNNGG 1 cut(s) 117
BseNI ACTGG 1 cut(s) 378
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 469
BslI CCNNNNNNNGG 1 cut(s) 117
BsmAI GTCTC 2 cut(s) 56, 521
BsnI GGCC 1 cut(s) 385
Bso31I GGTCTC 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 219, 493
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 1 cut(s) 225
BspANI GGCC 1 cut(s) 385
BspLI GGNNCC 1 cut(s) 492
BspMAI CTGCAG 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 521
BsrGI TGTACA 1 cut(s) 255
BsrI ACTGG 1 cut(s) 378
BssMI GATC 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 282
Bst6I CTCTTC 2 cut(s) 155, 327
BstACI GRCGYC 1 cut(s) 282
BstAUI TGTACA 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 44, 300
BstDEI CTNAG 1 cut(s) 495
BstF5I GGATG 1 cut(s) 491
BstKTI GATC 1 cut(s) 228
BstMAI GTCTC 2 cut(s) 56, 521
BstMBI GATC 1 cut(s) 225
BstMWI GCNNNNNNNGC 1 cut(s) 304
BstNSI RCATGY 2 cut(s) 97, 419
BstSCI CCNGG 1 cut(s) 468
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 160
BstV2I GAAGAC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 385
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 491
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 44, 300
Cfr13I GGNCC 2 cut(s) 190, 384
Csp6I GTAC 1 cut(s) 256
CviAII CATG 4 cut(s) 94, 404, 416, 424
CviJI RGCY 7 cut(s) 9, 298, 307, 346, 385, 473, 491
CviKI_1 RGCY 7 cut(s) 9, 298, 307, 346, 385, 473, 491
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 1 cut(s) 495
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
DraIII CACNNNGTG 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 155, 327
EarI CTCTTC 2 cut(s) 155, 327
Eco24I GRGCYC 1 cut(s) 493
Eco31I GGTCTC 1 cut(s) 521
Eco32I GATATC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 190
EcoRV GATATC 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 493
FaeI CATG 4 cut(s) 97, 407, 419, 427
FatI CATG 4 cut(s) 93, 403, 415, 423
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 498
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 385
HapII CCGG 1 cut(s) 469
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 4 cut(s) 97, 407, 419, 427
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 1 cut(s) 64
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 1 cut(s) 469
Hpy166II GTNNAC 2 cut(s) 217, 391
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 1 cut(s) 457
Hpy8I GTNNAC 2 cut(s) 217, 391
HpyAV CCTTC 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 6 cut(s) 42, 176, 217, 243, 366, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 304
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 1 cut(s) 282
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 4 cut(s) 97, 407, 419, 427
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 1 cut(s) 225
LmnI GCTCC 2 cut(s) 90, 496
LpnPI CCDG 5 cut(s) 28, 126, 263, 391, 482
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 1 cut(s) 72
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 316
MalI GATC 1 cut(s) 227
MboI GATC 1 cut(s) 225
MboII GAAGA 5 cut(s) 31, 142, 227, 314, 544
MhlI GDGCHC 2 cut(s) 219, 493
MluCI AATT 2 cut(s) 12, 433
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 5 cut(s) 113, 158, 258, 288, 476
MseI TTAA 4 cut(s) 11, 210, 390, 432
MslI CAYNNNNRTG 1 cut(s) 134
MspI CCGG 1 cut(s) 469
MspR9I CCNGG 1 cut(s) 470
MwoI GCNNNNNNNGC 1 cut(s) 304
NciI CCSGG 1 cut(s) 470
NdeII GATC 1 cut(s) 225
NlaIII CATG 4 cut(s) 97, 407, 419, 427
NlaIV GGNNCC 1 cut(s) 492
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 316
NspI RCATGY 2 cut(s) 97, 419
PciI ACATGT 1 cut(s) 93
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PscI ACATGT 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 2 cut(s) 190, 384
PstI CTGCAG 1 cut(s) 178
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 4 cut(s) 11, 210, 390, 432
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 2 cut(s) 190, 384
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 2 cut(s) 219, 493
SetI ASST 7 cut(s) 11, 146, 216, 250, 285, 309, 403
SfaNI GCATC 1 cut(s) 72
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 134
Sse9I AATT 2 cut(s) 12, 433
StyD4I CCNGG 1 cut(s) 468
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 250
TasI AATT 2 cut(s) 12, 433
TatI WGTACW 1 cut(s) 255
Tru1I TTAA 4 cut(s) 11, 210, 390, 432
Tru9I TTAA 4 cut(s) 11, 210, 390, 432
TscAI CASTG 1 cut(s) 192
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 170, 412
TspRI CASTG 1 cut(s) 192
VneI GTGCAC 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 190
XceI RCATGY 2 cut(s) 97, 419
ZraI GACGTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.