Rmu_co8400549.1_g000001

Alkaline ceramidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8400549.1
OG0008451
Physical Location & Seq
Forward (+)
126 .. 801
676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8400549.1_g000001.1.cds

Sequence Viewer

Length: 531 bp
atgcacgtccactttagtcaacagcagagcgatgagactcctatggtttgggagatgctcctatacatgtatatcctctactccccagattggcactatcgcactacaatgccgaccttcctcttcctatatggtgttgtttttgctgcagttcattcagtgttccgatttgagattggttttaaggtgcactatgtgatcttgtgtcttctttgcatacctcgaatgtacaagtattatatatacacacaggacgtctatgccaagaggctcgcaaagctgtatgtggccactcttcttatagggagtttgtgttggctcggcgacaatgttttctgcaaagagatatccagttggcccgttaacccacaaggtcatgccttgtggcatgtattcatgggttttaattcctattttgctaacactttcttgatgttttgccgggctgaacaacgaggatgggctcctaagattgttcgttttatgggtctcttgccctatgtgaagattgaaaaaccaaaaacccaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

176

Amino Acids

20.99

Weight (kDa)

9.06

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 90
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 1 cut(s) 512
AjiI CACGTC 1 cut(s) 7
AluBI AGCT 1 cut(s) 280
AluI AGCT 1 cut(s) 280
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 29, 494
Alw44I GTGCAC 1 cut(s) 188
AoxI GGCC 2 cut(s) 288, 356
ApaLI GTGCAC 1 cut(s) 188
ApeKI GCWGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 133
BccI CCATC 1 cut(s) 453
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 2 cut(s) 29, 494
BfmI CTRYAG 1 cut(s) 147
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 443
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 443
BmsI GCATC 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 255
BsaI GGTCTC 1 cut(s) 494
BsaXI ACNNNNNCTCC 2 cut(s) 298, 328
Bsc4I CCNNNNNNNGG 1 cut(s) 90
Bse1I ACTGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 1 cut(s) 90
BseNI ACTGG 1 cut(s) 351
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 1 cut(s) 133
BshFI GGCC 2 cut(s) 290, 358
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 90
BsmAI GTCTC 2 cut(s) 29, 494
BsnI GGCC 2 cut(s) 290, 358
Bso31I GGTCTC 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 192, 466
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 1 cut(s) 198
BspANI GGCC 2 cut(s) 290, 358
BspLI GGNNCC 1 cut(s) 465
BspMAI CTGCAG 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 494
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 1 cut(s) 351
BssMI GATC 1 cut(s) 198
BssNI GRCGYC 1 cut(s) 255
Bst6I CTCTTC 2 cut(s) 128, 300
BstACI GRCGYC 1 cut(s) 255
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 464
BstKTI GATC 1 cut(s) 201
BstMAI GTCTC 2 cut(s) 29, 494
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 1 cut(s) 277
BstNSI RCATGY 2 cut(s) 70, 392
BstSCI CCNGG 1 cut(s) 441
BstSFI CTRYAG 1 cut(s) 147
BstSLI GKGCMC 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 133
BstV2I GAAGAC 1 cut(s) 200
BsuRI GGCC 2 cut(s) 290, 358
BtgZI GCGATG 1 cut(s) 45
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 1 cut(s) 229
CviAII CATG 4 cut(s) 67, 377, 389, 397
CviJI RGCY 7 cut(s) 271, 280, 290, 319, 358, 446, 464
CviKI_1 RGCY 7 cut(s) 271, 280, 290, 319, 358, 446, 464
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 1 cut(s) 468
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
DraIII CACNNNGTG 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 128, 300
EarI CTCTTC 2 cut(s) 128, 300
Eco24I GRGCYC 1 cut(s) 466
Eco31I GGTCTC 1 cut(s) 494
Eco32I GATATC 1 cut(s) 348
EcoRV GATATC 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 466
FaeI CATG 4 cut(s) 70, 380, 392, 400
FatI CATG 4 cut(s) 66, 376, 388, 396
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 471
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 290, 358
HapII CCGG 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 4 cut(s) 70, 380, 392, 400
HincII GTYRAC 2 cut(s) 20, 364
HindII GTYRAC 2 cut(s) 20, 364
HinfI GANTC 1 cut(s) 37
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 1 cut(s) 442
Hpy166II GTNNAC 4 cut(s) 10, 20, 190, 364
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 1 cut(s) 430
Hpy8I GTNNAC 4 cut(s) 10, 20, 190, 364
HpyAV CCTTC 1 cut(s) 127
HpyCH4IV ACGT 2 cut(s) 6, 255
HpyCH4V TGCA 5 cut(s) 4, 149, 190, 216, 339
HpyF10VI GCNNNNNNNGC 1 cut(s) 277
HpyF3I CTNAG 1 cut(s) 468
HpySE526I ACGT 2 cut(s) 6, 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 4 cut(s) 70, 380, 392, 400
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 2 cut(s) 63, 469
LpnPI CCDG 4 cut(s) 99, 236, 364, 455
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 1 cut(s) 45
MaeII ACGT 2 cut(s) 6, 255
MalI GATC 1 cut(s) 200
MboI GATC 1 cut(s) 198
MboII GAAGA 4 cut(s) 115, 200, 287, 517
MhlI GDGCHC 2 cut(s) 192, 466
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 1 cut(s) 406
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 31
MnlI CCTC 5 cut(s) 86, 131, 231, 261, 449
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 3 cut(s) 183, 363, 405
MslI CAYNNNNRTG 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 290
MspI CCGG 1 cut(s) 442
MspR9I CCNGG 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 443
NdeII GATC 1 cut(s) 198
NlaIII CATG 4 cut(s) 70, 380, 392, 400
NlaIV GGNNCC 1 cut(s) 465
NmeAIII GCCGAG 1 cut(s) 300
NspI RCATGY 2 cut(s) 70, 392
PciI ACATGT 1 cut(s) 66
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PscI ACATGT 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 151
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 3 cut(s) 183, 363, 405
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 1 cut(s) 198
Sau96I GGNCC 1 cut(s) 357
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 2 cut(s) 192, 466
SetI ASST 7 cut(s) 9, 119, 189, 223, 258, 282, 376
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 107
Sse9I AATT 1 cut(s) 406
StyD4I CCNGG 1 cut(s) 441
TaiI ACGT 2 cut(s) 9, 258
TaqI TCGA 1 cut(s) 223
TasI AATT 1 cut(s) 406
TatI WGTACW 1 cut(s) 228
Tru1I TTAA 3 cut(s) 183, 363, 405
Tru9I TTAA 3 cut(s) 183, 363, 405
TscAI CASTG 1 cut(s) 165
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 143, 385
TspRI CASTG 1 cut(s) 165
VneI GTGCAC 1 cut(s) 188
XceI RCATGY 2 cut(s) 70, 392
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.