Prupe.4G011800_v2.0.a1

Alkaline ceramidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0008451
Physical Location & Seq
Reverse (-)
568534 .. 571398
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G011800.1

Sequence Viewer

Length: 768 bp
ATGGCTGATACCGGATCAAGCTTCTGGGGTCCTGTCACATCCACGATTGAATGTTGCGAGAAGAATTATGCCTACTCTTCTTATATTGCGGAATTTTTCAACACTTTATCAAACATCCCAAGCATTCTTTTGGCGCTCATTGGGCTTATAAATGTCTTAAGGCAGCGGTTTGAGAAGAGATTCAGCATTCTTCACATATCTAATATGATTCTTGCCATTGGAAGCATGTTATACCATGCCACGTTGCAGCATGTGCAACAGCAGAGCGATGAGACTCCTATGGTTTGGGAGATGCTCCTTTACATGTATATCCTCTACTCCCCAGATTGGCACTATCGCAGTACAATGCCCATCTTCCTCTTCCTCTATGGTGCTGTTTTTGCTGCTGTTCATTCTGTGGCTCGTTTTGAAATTGGCTTTAAGGTGCACTATGTGATCTTGTGCCTCCTCTGCATTCCTCGAATGTACAAGTATTATATATACACCCAAGACTTGTCTGCTAAGAGGATTGCAAAGCTGTATGTTGCCACCCTTCTTATAGGGAGTTTGTGTTGGCTCTGTGATAATGTTTACTGCGAAGAGATATCCAGTTGGACCATTAACCCGCAAGGTCATGCCTTGTGGCATTTGTTCATGGGTTTTAATTCCTACTTTGCAAACACTTTCTTGATGTTTTGCCGGGCTCAGCAACGAGGGTGGGGCCCCAAAGTAGTTCATTTTATGGGGGTTTTGCCCTATGTGAAGATTGAAAAACCGAAAACCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

256

Amino Acids

29.67

Weight (kDa)

8.55

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AciI CCGC 3 cut(s) 89, 166, 605
AclWI GGATC 1 cut(s) 22
AcsI RAATTY 1 cut(s) 92
AdeI CACNNNGTG 1 cut(s) 433
AfaI GTAC 2 cut(s) 343, 467
AfiI CCNNNNNNNGG 1 cut(s) 327
AflII CTTAAG 1 cut(s) 157
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 50, 100, 410, 749
AluBI AGCT 2 cut(s) 21, 517
AluI AGCT 2 cut(s) 21, 517
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 266
Alw44I GTGCAC 1 cut(s) 425
AlwI GGATC 1 cut(s) 22
AoxI GGCC 1 cut(s) 700
ApaI GGGCCC 1 cut(s) 704
ApaLI GTGCAC 1 cut(s) 425
ApeKI GCWGC 3 cut(s) 163, 247, 383
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 4 cut(s) 29, 594, 700, 701
AsuC2I CCSGG 1 cut(s) 680
AvaII GGWCC 2 cut(s) 29, 594
BaeGI GKGCMC 2 cut(s) 429, 704
BanII GRGCYC 2 cut(s) 685, 704
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 175, 259, 370
BccI CCATC 1 cut(s) 359
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 137
BfrI CTTAAG 1 cut(s) 157
BisI GCNGC 3 cut(s) 164, 248, 384
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 3 cut(s) 165, 249, 385
Bme1390I CCNGG 1 cut(s) 680
Bme18I GGWCC 2 cut(s) 29, 594
BmgT120I GGNCC 4 cut(s) 29, 594, 700, 701
BmiI GGNNCC 4 cut(s) 30, 701, 702, 703
BmrFI CCNGG 1 cut(s) 680
BmsI GCATC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 684
BpuMI CCSGG 1 cut(s) 680
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 588
BseGI GGATG 2 cut(s) 38, 114
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 1 cut(s) 588
BseRI GAGGAG 1 cut(s) 437
BseSI GKGCMC 2 cut(s) 429, 704
BseXI GCAGC 3 cut(s) 175, 259, 370
BshFI GGCC 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 2 cut(s) 12, 679
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 1 cut(s) 266
BsmI GAATGC 3 cut(s) 123, 186, 453
BsnI GGCC 1 cut(s) 702
Bsp120I GGGCCC 1 cut(s) 700
Bsp1286I GDGCHC 3 cut(s) 429, 685, 704
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 2 cut(s) 14, 435
Bsp1720I GCTNAGC 1 cut(s) 684
BspACI CCGC 3 cut(s) 89, 166, 605
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 1 cut(s) 697
BspLI GGNNCC 4 cut(s) 30, 701, 702, 703
BspPI GGATC 1 cut(s) 22
BspTI CTTAAG 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 1 cut(s) 588
BssMI GATC 2 cut(s) 14, 435
Bst6I CTCTTC 4 cut(s) 82, 170, 365, 573
BstAFI CTTAAG 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 465
BstDEI CTNAG 2 cut(s) 501, 684
BstF5I GGATG 2 cut(s) 38, 114
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 2 cut(s) 17, 438
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 2 cut(s) 14, 435
BstMWI GCNNNNNNNGC 4 cut(s) 142, 253, 380, 450
BstNSI RCATGY 3 cut(s) 229, 254, 307
BstSCI CCNGG 1 cut(s) 678
BstSLI GKGCMC 2 cut(s) 429, 704
BstV1I GCAGC 3 cut(s) 175, 259, 370
BsuRI GGCC 1 cut(s) 702
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 2 cut(s) 38, 114
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 4 cut(s) 29, 594, 700, 701
Csp6I GTAC 2 cut(s) 342, 466
CspCI CAANNNNNGTGG 2 cut(s) 677, 712
CviAII CATG 6 cut(s) 226, 236, 251, 304, 614, 634
CviJI RGCY 9 cut(s) 5, 21, 145, 401, 417, 517, 556, 683, 702
CviKI_1 RGCY 9 cut(s) 5, 21, 145, 401, 417, 517, 556, 683, 702
CviQI GTAC 2 cut(s) 342, 466
DdeI CTNAG 2 cut(s) 501, 684
DpnI GATC 2 cut(s) 16, 437
DpnII GATC 2 cut(s) 14, 435
DraIII CACNNNGTG 1 cut(s) 433
Eam1104I CTCTTC 4 cut(s) 82, 170, 365, 573
EarI CTCTTC 4 cut(s) 82, 170, 365, 573
Eco24I GRGCYC 2 cut(s) 685, 704
Eco32I GATATC 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 29, 594
EcoO109I RGGNCCY 3 cut(s) 29, 700, 701
EcoRV GATATC 1 cut(s) 585
EcoT38I GRGCYC 2 cut(s) 685, 704
FaeI CATG 6 cut(s) 229, 239, 254, 307, 617, 637
FatI CATG 6 cut(s) 225, 235, 250, 303, 613, 633
FauI CCCGC 1 cut(s) 612
Fnu4HI GCNGC 3 cut(s) 164, 248, 384
FokI GGATG 2 cut(s) 25, 101
FriOI GRGCYC 2 cut(s) 685, 704
Fsp4HI GCNGC 3 cut(s) 164, 248, 384
GlaI GCGC 1 cut(s) 135
GluI GCNGC 3 cut(s) 164, 248, 384
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 1 cut(s) 702
HapII CCGG 2 cut(s) 12, 679
HhaI GCGC 1 cut(s) 136
Hin1II CATG 6 cut(s) 229, 239, 254, 307, 617, 637
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HindIII AAGCTT 1 cut(s) 19
HinfI GANTC 3 cut(s) 180, 208, 274
HpaII CCGG 2 cut(s) 12, 679
Hpy166II GTNNAC 2 cut(s) 427, 571
Hpy188III TCNNGA 1 cut(s) 667
Hpy8I GTNNAC 2 cut(s) 427, 571
HpyAV CCTTC 1 cut(s) 542
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 6 cut(s) 247, 256, 427, 453, 512, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 253, 380, 450
HpyF3I CTNAG 2 cut(s) 501, 684
HpySE526I ACGT 1 cut(s) 242
Hsp92II CATG 6 cut(s) 229, 239, 254, 307, 617, 637
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 2 cut(s) 14, 435
LmnI GCTCC 1 cut(s) 300
LpnPI CCDG 6 cut(s) 10, 25, 45, 336, 601, 692
Lsp1109I GCAGC 3 cut(s) 175, 259, 370
LweI GCATC 1 cut(s) 282
MaeII ACGT 1 cut(s) 242
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 16, 437
MboI GATC 2 cut(s) 14, 435
MboII GAAGA 8 cut(s) 69, 73, 182, 187, 346, 352, 590, 754
MhlI GDGCHC 3 cut(s) 429, 685, 704
MluCI AATT 4 cut(s) 64, 92, 411, 643
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 8 cut(s) 323, 368, 374, 455, 458, 468, 498, 686
MroXI GAANNNNTTC 1 cut(s) 179
MseI TTAA 4 cut(s) 158, 420, 600, 642
MspA1I CMGCKG 1 cut(s) 166
MspCI CTTAAG 1 cut(s) 157
MspI CCGG 2 cut(s) 12, 679
MspR9I CCNGG 1 cut(s) 680
Mva1269I GAATGC 3 cut(s) 123, 186, 453
MwoI GCNNNNNNNGC 4 cut(s) 142, 253, 380, 450
NciI CCSGG 1 cut(s) 680
NdeII GATC 2 cut(s) 14, 435
NlaIII CATG 6 cut(s) 229, 239, 254, 307, 617, 637
NlaIV GGNNCC 4 cut(s) 30, 701, 702, 703
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 3 cut(s) 229, 254, 307
PciI ACATGT 1 cut(s) 303
PctI GAATGC 3 cut(s) 123, 186, 453
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 180, 208
PkrI GCNGC 3 cut(s) 165, 249, 385
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 29
PscI ACATGT 1 cut(s) 303
PsiI TTATAA 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 29
PspN4I GGNNCC 4 cut(s) 30, 701, 702, 703
PspOMI GGGCCC 1 cut(s) 700
PspPI GGNCC 4 cut(s) 29, 594, 700, 701
PspPPI RGGWCCY 1 cut(s) 29
RsaI GTAC 2 cut(s) 343, 467
RsaNI GTAC 2 cut(s) 342, 466
SaqAI TTAA 4 cut(s) 158, 420, 600, 642
SatI GCNGC 3 cut(s) 164, 248, 384
Sau3AI GATC 2 cut(s) 14, 435
Sau96I GGNCC 4 cut(s) 29, 594, 700, 701
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 3 cut(s) 429, 685, 704
SetI ASST 5 cut(s) 23, 245, 426, 519, 613
SfaNI GCATC 1 cut(s) 282
SinI GGWCC 2 cut(s) 29, 594
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 4 cut(s) 64, 92, 411, 643
SsiI CCGC 3 cut(s) 89, 166, 605
StyD4I CCNGG 1 cut(s) 678
TaiI ACGT 1 cut(s) 245
TaqI TCGA 1 cut(s) 460
TasI AATT 4 cut(s) 64, 92, 411, 643
TatI WGTACW 2 cut(s) 341, 465
TfiI GAWTC 2 cut(s) 180, 208
Tru1I TTAA 4 cut(s) 158, 420, 600, 642
Tru9I TTAA 4 cut(s) 158, 420, 600, 642
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 3 cut(s) 163, 247, 383
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 3 cut(s) 380, 622, 704
Vha464I CTTAAG 1 cut(s) 157
VneI GTGCAC 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 29, 594
XapI RAATTY 1 cut(s) 92
XceI RCATGY 3 cut(s) 229, 254, 307
XmnI GAANNNNTTC 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.