pycom05g32300

Alkaline ceramidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008451
Physical Location & Seq
Forward (+)
31752839 .. 31754175
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32300.2

Sequence Viewer

Length: 768 bp
ATGGCTGATGGAATATCAAGCTTCTGGGGTCCTGTCACATCAACAATTGAGTGTTGCGAAAAGAATTATGCCTACTCTTCTTATATTGCTGAATTTTACAACACTATATCAAACATTCCAGGCATTCTGTTGGCACTCATTGGCCTTATAAATGCCTTGAGGCAGCGGTTTGAGAAGAGATTTGGCATTCTTCACATATCTAATATGATTCTTGCCATCGGAAGCATGTTATACCATGCCACATTGCAACATGTGCAACAGCAGAGCGATGAGACTCCAATGGTTTGGGAGATGCTCCTTTACATGTATATACTCTACTCCCCAGATTGGCACTATAGGAGTACAATGCCCACCTTCCTCTTCCTCTATGGTGCTGTGTTCGCAGGTGTTCATTCTGTGGTTCGTTTTGAGATTGGCTTTAAGGTGCACTACATGATCTTGTGTCTTCTCTGCATACCTCGAATGTACAAGTACTACATATACACGCAAGACGTGTGTGCTAAGCGGATTGCAAAGCTGTATGTGGCCACCCTTGTTACGGGCAGTTTGTGTTGGCTCTGCGATAAGATCTTCTGCAAAGAGATATCCGGTTGGATGATTAACCCGCAGGGTCATGCCTTGTGGCATTTGTTCATGGGTTTCAATTCCTACTTTGCAAACACTTTCTTGATGTTTTGCCGGGCCCAGCAGCGAGGATGGTCCCCCAGGATTGTTCGTTTTATGGGGTTTTTGCCCTATGTGAAGATTGAGAAACCAAAAACCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

256

Amino Acids

29.77

Weight (kDa)

8.99

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AarI CACCTGC 1 cut(s) 374
Acc36I ACCTGC 1 cut(s) 374
AciI CCGC 3 cut(s) 166, 505, 605
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 92
AfaI GTAC 3 cut(s) 343, 467, 473
AfiI CCNNNNNNNGG 2 cut(s) 327, 538
AflIII ACRYGT 3 cut(s) 250, 303, 492
AgsI TTSAA 1 cut(s) 643
AjiI CACGTC 1 cut(s) 493
AjnI CCWGG 2 cut(s) 118, 704
AluBI AGCT 2 cut(s) 21, 517
AluI AGCT 2 cut(s) 21, 517
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 266
Alw44I GTGCAC 1 cut(s) 425
AoxI GGCC 3 cut(s) 142, 525, 681
ApaI GGGCCC 1 cut(s) 685
ApaLI GTGCAC 1 cut(s) 425
ApeKI GCWGC 2 cut(s) 163, 688
ApoI RAATTY 1 cut(s) 92
AspS9I GGNCC 4 cut(s) 29, 681, 682, 699
AsuC2I CCSGG 1 cut(s) 680
AvaII GGWCC 2 cut(s) 29, 699
BaeGI GKGCMC 2 cut(s) 429, 685
BalI TGGCCA 1 cut(s) 527
BanII GRGCYC 1 cut(s) 685
BbsI GAAGAC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 175, 700
BccI CCATC 3 cut(s) 2, 224, 690
BciT130I CCWGG 2 cut(s) 120, 706
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 374
BglII AGATCT 1 cut(s) 567
BisI GCNGC 2 cut(s) 164, 689
BlpI GCTNAGC 1 cut(s) 501
BlsI GCNGC 2 cut(s) 165, 690
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 3 cut(s) 120, 680, 706
Bme18I GGWCC 2 cut(s) 29, 699
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 4 cut(s) 29, 681, 682, 699
BmiI GGNNCC 3 cut(s) 30, 683, 701
BmrFI CCNGG 3 cut(s) 120, 680, 706
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 437
Bpu1102I GCTNAGC 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 680
BsaJI CCNNGG 1 cut(s) 704
BsaWI WCCGGW 1 cut(s) 587
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 538
Bse3DI GCAATG 1 cut(s) 242
BseBI CCWGG 2 cut(s) 120, 706
BseDI CCNNGG 1 cut(s) 704
BseGI GGATG 2 cut(s) 600, 701
BseLI CCNNNNNNNGG 2 cut(s) 327, 538
BseMI GCAATG 1 cut(s) 242
BseSI GKGCMC 2 cut(s) 429, 685
BseXI GCAGC 2 cut(s) 175, 700
BseYI CCCAGC 1 cut(s) 684
BshFI GGCC 3 cut(s) 144, 527, 683
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 2 cut(s) 588, 679
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 2 cut(s) 327, 538
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 685
BsmI GAATGC 2 cut(s) 123, 186
BsnI GGCC 3 cut(s) 144, 527, 683
Bsp120I GGGCCC 1 cut(s) 681
Bsp1286I GDGCHC 2 cut(s) 429, 685
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 2 cut(s) 435, 567
Bsp1720I GCTNAGC 1 cut(s) 501
BspACI CCGC 3 cut(s) 166, 505, 605
BspANI GGCC 3 cut(s) 144, 527, 683
BspLI GGNNCC 3 cut(s) 30, 683, 701
BspMI ACCTGC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 465
BssECI CCNNGG 1 cut(s) 704
BssMI GATC 2 cut(s) 435, 567
Bst2UI CCWGG 2 cut(s) 120, 706
Bst6I CTCTTC 3 cut(s) 82, 170, 365
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 465
BstDEI CTNAG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 600, 701
BstKTI GATC 2 cut(s) 438, 570
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 2 cut(s) 435, 567
BstMWI GCNNNNNNNGC 2 cut(s) 253, 380
BstNI CCWGG 2 cut(s) 120, 706
BstNSI RCATGY 3 cut(s) 229, 254, 307
BstSCI CCNGG 3 cut(s) 118, 678, 704
BstSFI CTRYAG 1 cut(s) 334
BstSLI GKGCMC 2 cut(s) 429, 685
BstV1I GCAGC 2 cut(s) 175, 700
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 567
BstXI CCANNNNNNTGG 1 cut(s) 285
BstYI RGATCY 1 cut(s) 567
BsuRI GGCC 3 cut(s) 144, 527, 683
BtgZI GCGATG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 2 cut(s) 600, 701
BveI ACCTGC 1 cut(s) 374
Cfr13I GGNCC 4 cut(s) 29, 681, 682, 699
Csp6I GTAC 3 cut(s) 342, 466, 472
CviAII CATG 7 cut(s) 226, 236, 251, 304, 433, 614, 634
CviJI RGCY 8 cut(s) 5, 21, 144, 417, 517, 527, 556, 683
CviKI_1 RGCY 8 cut(s) 5, 21, 144, 417, 517, 527, 556, 683
CviQI GTAC 3 cut(s) 342, 466, 472
DdeI CTNAG 1 cut(s) 501
DpnI GATC 2 cut(s) 437, 569
DpnII GATC 2 cut(s) 435, 567
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 3 cut(s) 82, 170, 365
EarI CTCTTC 3 cut(s) 82, 170, 365
Eco24I GRGCYC 1 cut(s) 685
Eco32I GATATC 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 29, 699
EcoO109I RGGNCCY 1 cut(s) 29
EcoRII CCWGG 2 cut(s) 118, 704
EcoRV GATATC 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 685
FaeI CATG 7 cut(s) 229, 239, 254, 307, 436, 617, 637
FaqI GGGAC 1 cut(s) 685
FatI CATG 7 cut(s) 225, 235, 250, 303, 432, 613, 633
FauI CCCGC 1 cut(s) 612
Fnu4HI GCNGC 2 cut(s) 164, 689
FokI GGATG 2 cut(s) 607, 708
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 2 cut(s) 164, 689
GluI GCNGC 2 cut(s) 164, 689
GsaI CCCAGC 1 cut(s) 688
HaeIII GGCC 3 cut(s) 144, 527, 683
HapII CCGG 2 cut(s) 588, 679
Hin1II CATG 7 cut(s) 229, 239, 254, 307, 436, 617, 637
HindIII AAGCTT 1 cut(s) 19
HinfI GANTC 2 cut(s) 208, 274
HpaII CCGG 2 cut(s) 588, 679
Hpy166II GTNNAC 1 cut(s) 427
Hpy188I TCNGA 1 cut(s) 221
Hpy188III TCNNGA 1 cut(s) 667
Hpy8I GTNNAC 1 cut(s) 427
HpyAV CCTTC 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 7 cut(s) 247, 256, 427, 453, 512, 576, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 380
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 7 cut(s) 229, 239, 254, 307, 436, 617, 637
Kzo9I GATC 2 cut(s) 435, 567
LmnI GCTCC 1 cut(s) 300
Lsp1109I GCAGC 2 cut(s) 175, 700
LweI GCATC 1 cut(s) 282
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 2 cut(s) 34, 535
MalI GATC 2 cut(s) 437, 569
MboI GATC 2 cut(s) 435, 567
MboII GAAGA 7 cut(s) 69, 182, 187, 352, 437, 562, 754
MfeI CAATTG 1 cut(s) 45
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 429, 685
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 4 cut(s) 45, 64, 92, 643
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 5 cut(s) 153, 368, 374, 468, 686
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 2 cut(s) 420, 600
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 2 cut(s) 588, 679
MspR9I CCNGG 3 cut(s) 120, 680, 706
MunI CAATTG 1 cut(s) 45
Mva1269I GAATGC 2 cut(s) 123, 186
MvaI CCWGG 2 cut(s) 120, 706
MwoI GCNNNNNNNGC 2 cut(s) 253, 380
NciI CCSGG 1 cut(s) 680
NdeII GATC 2 cut(s) 435, 567
NlaIII CATG 7 cut(s) 229, 239, 254, 307, 436, 617, 637
NlaIV GGNNCC 3 cut(s) 30, 683, 701
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 3 cut(s) 229, 254, 307
PaqCI CACCTGC 1 cut(s) 374
PciI ACATGT 2 cut(s) 250, 303
PctI GAATGC 2 cut(s) 123, 186
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 2 cut(s) 165, 690
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 29
PscI ACATGT 2 cut(s) 250, 303
PsiI TTATAA 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 118, 704
PspFI CCCAGC 1 cut(s) 684
PspGI CCWGG 2 cut(s) 118, 704
PspN4I GGNNCC 3 cut(s) 30, 683, 701
PspOMI GGGCCC 1 cut(s) 681
PspPI GGNCC 4 cut(s) 29, 681, 682, 699
PspPPI RGGWCCY 1 cut(s) 29
PsuI RGATCY 1 cut(s) 567
RsaI GTAC 3 cut(s) 343, 467, 473
RsaNI GTAC 3 cut(s) 342, 466, 472
SaqAI TTAA 2 cut(s) 420, 600
SatI GCNGC 2 cut(s) 164, 689
Sau3AI GATC 2 cut(s) 435, 567
Sau96I GGNCC 4 cut(s) 29, 681, 682, 699
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 3 cut(s) 120, 680, 706
SduI GDGCHC 2 cut(s) 429, 685
SetI ASST 7 cut(s) 23, 356, 388, 426, 460, 495, 519
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 334
SinI GGWCC 2 cut(s) 29, 699
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 4 cut(s) 45, 64, 92, 643
SsiI CCGC 3 cut(s) 166, 505, 605
StyD4I CCNGG 3 cut(s) 118, 678, 704
TaiI ACGT 1 cut(s) 495
TaqI TCGA 1 cut(s) 460
TasI AATT 4 cut(s) 45, 64, 92, 643
TatI WGTACW 3 cut(s) 341, 465, 471
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 2 cut(s) 420, 600
Tru9I TTAA 2 cut(s) 420, 600
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 2 cut(s) 163, 688
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 380, 622
VneI GTGCAC 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 29, 699
XapI RAATTY 1 cut(s) 92
XceI RCATGY 3 cut(s) 229, 254, 307
ZrmI AGTACT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.