Rw5G001230

Alkaline ceramidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008451
Physical Location & Seq
Reverse (-)
990472 .. 991875
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001230.1

Sequence Viewer

Length: 732 bp
ATGGCTGATGCGATATCGAGCTTCTGGGGTCCTGTCACATCAACGATCGAGTGTTGTGAGAAGAACTATGCCTACTCTTCTTATATTGCGGAATTTTTCAACACCATGTCGAACATTCCCAGCATACTCTTGGCACTCATTGGTCTTATAAATGCTCTAAGGCAGCGGTTTGAGAAGAGATTCAGTGTTCTTCATATATCTAATATGATACTTGCCATTGGGAGCATGTTATACCACGCTACACTGCAGCACGTTCAACAGCAGAGCGATGAGACTCCTATGGTTTGGGAGATGCTCCTATACATGTATATCCTCTACTCCCCAGATTGGCACTATCGCACTACAATGCCGACCTTCCTCTTCCTATATGGTGTTATTGGTTTTAAGGTGCACTATGTGATCTTGTGTCTTCTTTGCATACCTCGAATGTACAAGTATTATATATACACACAGGACGTCTATGCCAAGAGGCTCGCAAAGCTGTATGTGGCCACTCTTCTTATAGGGAGTTTGTGTTGGCTCGGCGACAATGTTTTCTGCAAAGAGATATCCAGTTGGCCTGTTAACCCACAAGGTCATGCCTTGTGGCATGTATTCATGGGTTTTAATTCCTATTTTGCCAACACTTTCTTGATGTTTTGCCGGGCTGAACAACGAGGATGGGCTCCTAAGATTGTTCGTTTTATGGGTCTCTTGCCCTATGTGAAGATTGAAAAACCAAAAACCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

243

Amino Acids

28.42

Weight (kDa)

8.87

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 10 - 227 5.5e-55 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AatII GACGTC 1 cut(s) 459
AciI CCGC 2 cut(s) 89, 166
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 456
AdeI CACNNNGTG 1 cut(s) 397
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 327
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 3 cut(s) 100, 257, 713
AluBI AGCT 2 cut(s) 21, 481
AluI AGCT 2 cut(s) 21, 481
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 2 cut(s) 266, 695
Alw44I GTGCAC 1 cut(s) 389
AoxI GGCC 2 cut(s) 489, 557
ApaLI GTGCAC 1 cut(s) 389
ApeKI GCWGC 2 cut(s) 163, 247
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 29
AsuC2I CCSGG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 29
BaeGI GKGCMC 1 cut(s) 393
BalI TGGCCA 1 cut(s) 491
BanII GRGCYC 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 175, 259
BccI CCATC 1 cut(s) 654
BcnI CCSGG 1 cut(s) 644
BcoDI GTCTC 2 cut(s) 266, 695
BfmI CTRYAG 1 cut(s) 245
BisI GCNGC 2 cut(s) 164, 248
BlsI GCNGC 2 cut(s) 165, 249
Bme1390I CCNGG 1 cut(s) 644
Bme18I GGWCC 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 2 cut(s) 30, 666
BmrFI CCNGG 1 cut(s) 644
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 644
BsaHI GRCGYC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 695
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 552
BseGI GGATG 1 cut(s) 665
BseLI CCNNNNNNNGG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 552
BseSI GKGCMC 1 cut(s) 393
BseXI GCAGC 2 cut(s) 175, 259
BseYI CCCAGC 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 491, 559
BsiEI CGRYCG 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 1 cut(s) 643
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 2 cut(s) 266, 695
BsnI GGCC 2 cut(s) 491, 559
Bso31I GGTCTC 1 cut(s) 695
Bsp1286I GDGCHC 2 cut(s) 393, 667
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 2 cut(s) 45, 399
BspACI CCGC 2 cut(s) 89, 166
BspANI GGCC 2 cut(s) 491, 559
BspLI GGNNCC 2 cut(s) 30, 666
BspMAI CTGCAG 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 695
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 1 cut(s) 552
BssMI GATC 2 cut(s) 45, 399
BssNI GRCGYC 1 cut(s) 456
Bst6I CTCTTC 4 cut(s) 82, 170, 365, 501
BstACI GRCGYC 1 cut(s) 456
BstAUI TGTACA 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 158, 669
BstF5I GGATG 1 cut(s) 665
BstKTI GATC 2 cut(s) 48, 402
BstMAI GTCTC 2 cut(s) 266, 695
BstMBI GATC 2 cut(s) 45, 399
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 1 cut(s) 478
BstNSI RCATGY 3 cut(s) 229, 307, 593
BstSCI CCNGG 1 cut(s) 642
BstSFI CTRYAG 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 175, 259
BstV2I GAAGAC 1 cut(s) 401
BsuRI GGCC 2 cut(s) 491, 559
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 1 cut(s) 665
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 2 cut(s) 190, 242
Cac8I GCNNGC 1 cut(s) 474
Cfr13I GGNCC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 430
CviAII CATG 6 cut(s) 106, 226, 304, 578, 590, 598
CviJI RGCY 9 cut(s) 5, 21, 472, 481, 491, 520, 559, 647, 665
CviKI_1 RGCY 9 cut(s) 5, 21, 472, 481, 491, 520, 559, 647, 665
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 2 cut(s) 158, 669
DpnI GATC 2 cut(s) 47, 401
DpnII GATC 2 cut(s) 45, 399
DraIII CACNNNGTG 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 4 cut(s) 82, 170, 365, 501
EarI CTCTTC 4 cut(s) 82, 170, 365, 501
Eco24I GRGCYC 1 cut(s) 667
Eco31I GGTCTC 1 cut(s) 695
Eco32I GATATC 2 cut(s) 15, 549
Eco47I GGWCC 1 cut(s) 29
EcoO109I RGGNCCY 1 cut(s) 29
EcoRV GATATC 2 cut(s) 15, 549
EcoT38I GRGCYC 1 cut(s) 667
FaeI CATG 6 cut(s) 109, 229, 307, 581, 593, 601
FatI CATG 6 cut(s) 105, 225, 303, 577, 589, 597
Fnu4HI GCNGC 2 cut(s) 164, 248
FokI GGATG 1 cut(s) 672
FriOI GRGCYC 1 cut(s) 667
Fsp4HI GCNGC 2 cut(s) 164, 248
GluI GCNGC 2 cut(s) 164, 248
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 2 cut(s) 491, 559
HapII CCGG 1 cut(s) 643
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 6 cut(s) 109, 229, 307, 581, 593, 601
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 2 cut(s) 180, 274
HpaI GTTAAC 1 cut(s) 565
HpaII CCGG 1 cut(s) 643
Hpy166II GTNNAC 2 cut(s) 391, 565
Hpy188III TCNNGA 1 cut(s) 631
Hpy8I GTNNAC 2 cut(s) 391, 565
HpyAV CCTTC 1 cut(s) 364
HpyCH4IV ACGT 2 cut(s) 252, 456
HpyCH4V TGCA 4 cut(s) 247, 391, 417, 540
HpyF10VI GCNNNNNNNGC 1 cut(s) 478
HpyF3I CTNAG 2 cut(s) 158, 669
HpySE526I ACGT 2 cut(s) 252, 456
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 6 cut(s) 109, 229, 307, 581, 593, 601
KspAI GTTAAC 1 cut(s) 565
Kzo9I GATC 2 cut(s) 45, 399
LmnI GCTCC 3 cut(s) 222, 300, 670
LpnPI CCDG 8 cut(s) 10, 45, 133, 336, 437, 565, 573, 656
Lsp1109I GCAGC 2 cut(s) 175, 259
LweI GCATC 1 cut(s) 282
MaeII ACGT 2 cut(s) 252, 456
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 47, 401
MboI GATC 2 cut(s) 45, 399
MboII GAAGA 8 cut(s) 69, 73, 182, 187, 352, 401, 488, 718
MhlI GDGCHC 2 cut(s) 393, 667
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 2 cut(s) 92, 607
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 1 cut(s) 268
MnlI CCTC 5 cut(s) 323, 368, 432, 462, 650
Mox20I TGGCCA 1 cut(s) 491
MroXI GAANNNNTTC 1 cut(s) 179
MscI TGGCCA 1 cut(s) 491
MseI TTAA 3 cut(s) 384, 564, 606
MslI CAYNNNNRTG 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 1 cut(s) 643
MspR9I CCNGG 1 cut(s) 644
MwoI GCNNNNNNNGC 1 cut(s) 478
NciI CCSGG 1 cut(s) 644
NdeII GATC 2 cut(s) 45, 399
NlaIII CATG 6 cut(s) 109, 229, 307, 581, 593, 601
NlaIV GGNNCC 2 cut(s) 30, 666
NmeAIII GCCGAG 1 cut(s) 501
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 3 cut(s) 229, 307, 593
PciI ACATGT 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 1 cut(s) 180
PkrI GCNGC 2 cut(s) 165, 249
Ple19I CGATCG 1 cut(s) 48
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 29
PscI ACATGT 1 cut(s) 303
PsiI TTATAA 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 30, 666
PspPI GGNCC 1 cut(s) 29
PspPPI RGGWCCY 1 cut(s) 29
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 1 cut(s) 249
PvuI CGATCG 1 cut(s) 48
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 3 cut(s) 384, 564, 606
SatI GCNGC 2 cut(s) 164, 248
Sau3AI GATC 2 cut(s) 45, 399
Sau96I GGNCC 1 cut(s) 29
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 1 cut(s) 644
SduI GDGCHC 2 cut(s) 393, 667
SetI ASST 8 cut(s) 23, 255, 356, 390, 424, 459, 483, 577
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 245
SinI GGWCC 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 2 cut(s) 92, 607
SsiI CCGC 2 cut(s) 89, 166
StyD4I CCNGG 1 cut(s) 642
TaiI ACGT 2 cut(s) 255, 459
TaqI TCGA 4 cut(s) 17, 48, 110, 424
TasI AATT 2 cut(s) 92, 607
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 3 cut(s) 384, 564, 606
Tru9I TTAA 3 cut(s) 384, 564, 606
TscAI CASTG 2 cut(s) 190, 249
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 2 cut(s) 163, 247
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 182, 586
TspRI CASTG 2 cut(s) 190, 249
VneI GTGCAC 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 29
XapI RAATTY 1 cut(s) 92
XceI RCATGY 3 cut(s) 229, 307, 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 179
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.