Rroxscaffold_1G00074700

Alkaline ceramidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008451
Physical Location & Seq
Forward (+)
95597814 .. 95600216
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00074700.1

Sequence Viewer

Length: 768 bp
ATGGCTGATGCGATATCGAGCTTCTGGGGTCCTGTCACATCAACGATCGAGTGTTGTGAGAAGAACTATGCCTACTCTTCTTATATTGCGGAATTTTTCAACACCATGTCGAACATTCCCAGCATACTCTTGGCACTCATTGGTCTTATAAATGCTCTAAGGCAGCGGTTTGAGAAGAGATTCAGTGTTCTTCATATATCTAATATGATACTCGCCATTGGGAGCATGTTATACCACGCTACACTGCAGCACGTGCAACAGCAGAGCGATGAGACTCCTATGGTTTGGGAGATGCTCCTATACATGTATATCCTCTACTCCCCAGATTGGCACTATCGCACTACAATGCCGACCTTCCTCTTCCTATATGGTGTTGTTTTTGCTGCAGTTCATTCAGTGGTCCGATTTGAGATTGGTTTTAAGGTGCACTATGTGATCTTGTGTCTTCTTTGCATACCTCGAATGTACAAGTATTATATATACACACAGGACTTCTATGCCAAGAGGCTCGCAAAGCTGTATGTGGCCACTCTTCTTATAGGGAGTTTGTGTTGGCTCGGTGACAATGTTTTCTGCAAAGAGATATCCAGTTGGCCCATTAACCCGCAAGGTCATGCCTTGTGGCATGTATTCATGGGTTTTAATTCCTATTTTGCAAACACTTTCTTGATGTTTTGCCGGGCTGAACAACGAGGATGGGCTCCTAAGATTGTTTGTTTTATGGGTCTCTTGCCCTATGTGAAGATTGAAAAACCAAAAACCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0001101 GO:0002238 GO:0002376 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0006066 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006671 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008152 GO:0008284 GO:0008610 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009814 GO:0009987 GO:0010025 GO:0010033 GO:0010119 GO:0010166 GO:0012505 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0016810 GO:0016811 GO:0017040 GO:0019751 GO:0030104 GO:0030148 GO:0030173 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031228 GO:0031300 GO:0031301 GO:0031984 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0042127 GO:0042175 GO:0042221 GO:0042592 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046165 GO:0046173 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0048518 GO:0048522 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0070774 GO:0071602 GO:0071704 GO:0090333 GO:0097164 GO:0097305 GO:0098542 GO:0098588 GO:0098791 GO:0098827 GO:1901564 GO:1901566 GO:1901568 GO:1901570 GO:1901576 GO:1901615 GO:1901617 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00600 map00600
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

255

Amino Acids

29.77

Weight (kDa)

8.7

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 10 - 237 1e-62 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AciI CCGC 3 cut(s) 89, 166, 605
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 92
AcvI CACGTG 1 cut(s) 253
AdeI CACNNNGTG 1 cut(s) 433
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 1 cut(s) 327
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 2 cut(s) 100, 749
AluBI AGCT 2 cut(s) 21, 517
AluI AGCT 2 cut(s) 21, 517
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 266, 731
Alw44I GTGCAC 1 cut(s) 425
AoxI GGCC 2 cut(s) 525, 593
ApaLI GTGCAC 1 cut(s) 425
ApeKI GCWGC 3 cut(s) 163, 247, 383
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 179
AspS9I GGNCC 3 cut(s) 29, 400, 594
AsuC2I CCSGG 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 572
AvaII GGWCC 2 cut(s) 29, 400
BaeGI GKGCMC 1 cut(s) 429
BalI TGGCCA 1 cut(s) 527
BanII GRGCYC 1 cut(s) 703
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 175, 259, 370
BccI CCATC 1 cut(s) 690
BcnI CCSGG 1 cut(s) 680
BcoDI GTCTC 2 cut(s) 266, 731
BfmI CTRYAG 2 cut(s) 245, 384
BisI GCNGC 3 cut(s) 164, 248, 384
BlsI GCNGC 3 cut(s) 165, 249, 385
Bme1390I CCNGG 1 cut(s) 680
Bme18I GGWCC 2 cut(s) 29, 400
BmgT120I GGNCC 3 cut(s) 29, 400, 594
BmiI GGNNCC 2 cut(s) 30, 702
BmrFI CCNGG 1 cut(s) 680
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 680
BsaAI YACGTR 1 cut(s) 253
BsaI GGTCTC 1 cut(s) 731
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 588
BseGI GGATG 1 cut(s) 701
BseLI CCNNNNNNNGG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 588
BseSI GKGCMC 1 cut(s) 429
BseXI GCAGC 3 cut(s) 175, 259, 370
BseYI CCCAGC 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 527, 595
BsiEI CGRYCG 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 1 cut(s) 679
BslI CCNNNNNNNGG 1 cut(s) 327
BsmAI GTCTC 2 cut(s) 266, 731
BsnI GGCC 2 cut(s) 527, 595
Bso31I GGTCTC 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 429, 703
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 2 cut(s) 45, 435
BspACI CCGC 3 cut(s) 89, 166, 605
BspANI GGCC 2 cut(s) 527, 595
BspLI GGNNCC 2 cut(s) 30, 702
BspMAI CTGCAG 2 cut(s) 249, 388
BspTNI GGTCTC 1 cut(s) 731
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 1 cut(s) 588
BssMI GATC 2 cut(s) 45, 435
Bst6I CTCTTC 4 cut(s) 82, 170, 365, 537
BstAPI GCANNNNNTGC 1 cut(s) 253
BstAUI TGTACA 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 2 cut(s) 158, 705
BstF5I GGATG 1 cut(s) 701
BstKTI GATC 2 cut(s) 48, 438
BstMAI GTCTC 2 cut(s) 266, 731
BstMBI GATC 2 cut(s) 45, 435
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 2 cut(s) 253, 514
BstNSI RCATGY 3 cut(s) 229, 307, 629
BstSCI CCNGG 1 cut(s) 678
BstSFI CTRYAG 2 cut(s) 245, 384
BstSLI GKGCMC 1 cut(s) 429
BstV1I GCAGC 3 cut(s) 175, 259, 370
BstV2I GAAGAC 1 cut(s) 437
BsuRI GGCC 2 cut(s) 527, 595
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 1 cut(s) 701
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 3 cut(s) 190, 242, 402
Cac8I GCNNGC 1 cut(s) 510
Cfr13I GGNCC 3 cut(s) 29, 400, 594
Csp6I GTAC 1 cut(s) 466
CviAII CATG 6 cut(s) 106, 226, 304, 614, 626, 634
CviJI RGCY 9 cut(s) 5, 21, 508, 517, 527, 556, 595, 683, 701
CviKI_1 RGCY 9 cut(s) 5, 21, 508, 517, 527, 556, 595, 683, 701
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 2 cut(s) 158, 705
DpnI GATC 2 cut(s) 47, 437
DpnII GATC 2 cut(s) 45, 435
DraIII CACNNNGTG 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 4 cut(s) 82, 170, 365, 537
EarI CTCTTC 4 cut(s) 82, 170, 365, 537
Eco24I GRGCYC 1 cut(s) 703
Eco31I GGTCTC 1 cut(s) 731
Eco32I GATATC 2 cut(s) 15, 585
Eco47I GGWCC 2 cut(s) 29, 400
Eco72I CACGTG 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 29
EcoRV GATATC 2 cut(s) 15, 585
EcoT38I GRGCYC 1 cut(s) 703
FaeI CATG 6 cut(s) 109, 229, 307, 617, 629, 637
FatI CATG 6 cut(s) 105, 225, 303, 613, 625, 633
FauI CCCGC 1 cut(s) 612
Fnu4HI GCNGC 3 cut(s) 164, 248, 384
FokI GGATG 1 cut(s) 708
FriOI GRGCYC 1 cut(s) 703
Fsp4HI GCNGC 3 cut(s) 164, 248, 384
GluI GCNGC 3 cut(s) 164, 248, 384
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 2 cut(s) 527, 595
HapII CCGG 1 cut(s) 679
Hin1II CATG 6 cut(s) 109, 229, 307, 617, 629, 637
HinfI GANTC 2 cut(s) 180, 274
HpaII CCGG 1 cut(s) 679
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 1 cut(s) 427
Hpy188I TCNGA 1 cut(s) 404
Hpy188III TCNNGA 1 cut(s) 667
Hpy8I GTNNAC 1 cut(s) 427
HpyAV CCTTC 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 7 cut(s) 247, 256, 386, 427, 453, 576, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 514
HpyF3I CTNAG 2 cut(s) 158, 705
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 6 cut(s) 109, 229, 307, 617, 629, 637
Kzo9I GATC 2 cut(s) 45, 435
LmnI GCTCC 3 cut(s) 222, 300, 706
LpnPI CCDG 7 cut(s) 10, 45, 133, 336, 473, 601, 692
Lsp1109I GCAGC 3 cut(s) 175, 259, 370
LweI GCATC 1 cut(s) 282
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 34, 560
MalI GATC 2 cut(s) 47, 437
MboI GATC 2 cut(s) 45, 435
MboII GAAGA 8 cut(s) 69, 73, 182, 187, 352, 437, 524, 754
MhlI GDGCHC 2 cut(s) 429, 703
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 2 cut(s) 92, 643
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 1 cut(s) 268
MnlI CCTC 5 cut(s) 323, 368, 468, 498, 686
Mox20I TGGCCA 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 179
MscI TGGCCA 1 cut(s) 527
MseI TTAA 3 cut(s) 420, 600, 642
MslI CAYNNNNRTG 1 cut(s) 344
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 1 cut(s) 679
MspR9I CCNGG 1 cut(s) 680
MwoI GCNNNNNNNGC 2 cut(s) 253, 514
NciI CCSGG 1 cut(s) 680
NdeII GATC 2 cut(s) 45, 435
NlaIII CATG 6 cut(s) 109, 229, 307, 617, 629, 637
NlaIV GGNNCC 2 cut(s) 30, 702
NmuCI GTSAC 2 cut(s) 34, 560
NspI RCATGY 3 cut(s) 229, 307, 629
PciI ACATGT 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 179
PfeI GAWTC 1 cut(s) 180
PkrI GCNGC 3 cut(s) 165, 249, 385
Ple19I CGATCG 1 cut(s) 48
PleI GAGTC 1 cut(s) 268
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
PpsI GAGTC 1 cut(s) 268
Ppu21I YACGTR 1 cut(s) 253
PpuMI RGGWCCY 1 cut(s) 29
PscI ACATGT 1 cut(s) 303
PsiI TTATAA 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 29
PspCI CACGTG 1 cut(s) 253
PspFI CCCAGC 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 30, 702
PspPI GGNCC 3 cut(s) 29, 400, 594
PspPPI RGGWCCY 1 cut(s) 29
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 2 cut(s) 249, 388
PvuI CGATCG 1 cut(s) 48
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 3 cut(s) 420, 600, 642
SatI GCNGC 3 cut(s) 164, 248, 384
Sau3AI GATC 2 cut(s) 45, 435
Sau96I GGNCC 3 cut(s) 29, 400, 594
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 2 cut(s) 429, 703
SetI ASST 7 cut(s) 23, 255, 356, 426, 460, 519, 613
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 245, 384
SinI GGWCC 2 cut(s) 29, 400
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 2 cut(s) 92, 643
SsiI CCGC 3 cut(s) 89, 166, 605
StyD4I CCNGG 1 cut(s) 678
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 17, 48, 110, 460
TasI AATT 2 cut(s) 92, 643
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 3 cut(s) 420, 600, 642
Tru9I TTAA 3 cut(s) 420, 600, 642
TscAI CASTG 3 cut(s) 190, 249, 402
TseFI GTSAC 2 cut(s) 34, 560
TseI GCWGC 3 cut(s) 163, 247, 383
Tsp45I GTSAC 2 cut(s) 34, 560
TspDTI ATGAA 3 cut(s) 182, 380, 622
TspRI CASTG 3 cut(s) 190, 249, 402
VneI GTGCAC 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 29, 400
XapI RAATTY 1 cut(s) 92
XceI RCATGY 3 cut(s) 229, 307, 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.