AT4G23500

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008948
Physical Location & Seq
Forward (+)
12264462 .. 12267285
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23500.1

Sequence Viewer

Length: 1488 bp
ATGACCTCTCTCTCCACCGCCGGTTACCTTCTTCTCCTTCTCCTTCTCCCTATAAGCTACGACGGTGGCTTCATCGGAGCCGCCGATGCAGAACATGACCTCAGAGCCACTGATCTCCATAATCATCGCCATCGTCGCCACCGTAACCACCATCGCGGAGAAGAGTTTGAGTACTCAGCCATATCATGTAGAGCTTACTCAGCCTCATTGGACGAGTTTGGCGCCGTAGGAGACGGTGTAACTTCAAATACAGCGGCGTTTCGCGACGCGGTGTCACAACTTAGCCGTTTCGCAGACTACGGTGGCTCATTGTTATTTGTTCCCGCCGGACGGTGGCTCACCGGAAACTTTAACCTGACCAGCCATTTTACGCTTTTCCTCCACCGTGATGCTGTTATCCTCGCCTCTCAAGAAGAGAGTGACTATGAAGTGATAGAGCCACTGCCTTCGTACGGAAGAGGGCGAGACACAGATGGTGGAAGGTTCATCAGTCTACTTTTTGGTTCAAACTTAACCGATGTTGTTATCACCGGTGAGAATGGGACAATTGATGGACAAGGAGAACCATGGTGGGGGAAATTCAAGAGAGGAGAACTAAAATACACAAGACCTTATTTGATTGAGATTATGCATTCTGATGGTATCCAAATCTCCAATCTCACTTTCTTGAATTCTCCTTCTTGGCATATTCACCCGGTCTACAGCAGCAATATATATATCCAAGGTTTGACGATATTAGCACCGGTCACGGTACCAAACACTGACGGTATCAACCCAGATTCTTGTACCAACACGAGGATTGAGGACTGCTACATTGTCTCCGGCGACGATTGTATCGCCGTTAAAAGTGGATGGGATCAATACGGAATCAACTACGGCATGCCTACGAAACAGCTCTTGATCCGACGGCTCACATGCATTTCTCCTGATAGTGCCGTGATCGCTCTCGGCAGTGAAATGTCCGGTGGAATTGAAGATGTTCGAGCTGAAGATATTGTTGCCATTAACTCTGAATCTGGAATCAGGATTAAAACAGCTATCGGACGAGGAGGTTACGTGAAGGATGTATACGTAAGAGGAATGACAATGATGACAATGAAATATGTGTTTTGGATGACCGGAAGTTACGGTTCGCATCCGGACGATCATTATGATCCGAAAGCGTTACCGGTGATTCAGAACATTAATTATCAAGACATGGTCGCCGAGAACGTGACGATGCCTGCTCAGTTAGCTGGAATATCGGGAGATCAGTTTACTGGAATTTGTATCTCTAACGTGACTATCACTCTATCGAAGAAACCTAAGAAAGTGCTTTGGAATTGTACTGATGTTTCGGGTTATACTAGTGGTGTGACTCCGCAGCCGTGTCAGTTGTTACCGGAGAAGCAGCCGGGGACGGTTGTGCCGTGTAATTTCCCGGAAGATCCGATTCCTATTGATGAAGTGAAGCTTCAACGTTGCTACTCCAGAAGAAGGAATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

495

Amino Acids

54.69

Weight (kDa)

5.53

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 166 - 445 8.8e-34 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 751
AccB1I GGYRCC 2 cut(s) 221, 751
AccI GTMKAC 3 cut(s) 493, 699, 1068
AccII CGCG 3 cut(s) 156, 264, 269
AccIII TCCGGA 1 cut(s) 1138
AciI CCGC 7 cut(s) 18, 81, 156, 254, 269, 324, 1361
AclI AACGTT 1 cut(s) 1459
AclWI GGATC 4 cut(s) 864, 895, 1148, 1421
AcsI RAATTY 3 cut(s) 578, 670, 1263
AcuI CTGAAG 1 cut(s) 1008
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 5 cut(s) 173, 452, 753, 787, 1327
AfiI CCNNNNNNNGG 6 cut(s) 330, 333, 452, 572, 795, 1476
AgeI ACCGGT 3 cut(s) 530, 742, 1168
AgsI TTSAA 6 cut(s) 246, 507, 583, 670, 974, 1457
AhdI GACNNNNNGTC 1 cut(s) 271
AhlI ACTAGT 1 cut(s) 1346
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 7 cut(s) 57, 194, 895, 986, 1037, 1235, 1453
AluI AGCT 7 cut(s) 57, 194, 895, 986, 1037, 1235, 1453
Alw26I GTCTC 3 cut(s) 225, 459, 823
AlwI GGATC 4 cut(s) 864, 895, 1148, 1421
Aor13HI TCCGGA 1 cut(s) 1138
ApeKI GCWGC 3 cut(s) 705, 1363, 1390
ApoI RAATTY 3 cut(s) 578, 670, 1263
AseI ATTAAT 1 cut(s) 1185
AsiGI ACCGGT 3 cut(s) 530, 742, 1168
Asp700I GAANNNNTTC 1 cut(s) 978
Asp718I GGTACC 1 cut(s) 751
AspLEI GCGC 1 cut(s) 224
AsuC2I CCSGG 3 cut(s) 695, 1395, 1421
AsuHPI GGTGA 5 cut(s) 331, 520, 545, 683, 1183
BaeI ACNNNNGTAYC 2 cut(s) 817, 850
BanI GGYRCC 2 cut(s) 221, 751
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
BauI CACGAG 1 cut(s) 793
BbvI GCAGC 3 cut(s) 717, 1375, 1402
BccI CCATC 6 cut(s) 138, 159, 467, 545, 632, 846
BceAI ACGGC 8 cut(s) 209, 270, 824, 892, 920, 923, 1351, 1393
BciVI GTATCC 1 cut(s) 653
BcnI CCSGG 3 cut(s) 695, 1395, 1421
BcoDI GTCTC 3 cut(s) 225, 459, 823
BcuI ACTAGT 1 cut(s) 1346
BfaI CTAG 1 cut(s) 1347
BfmI CTRYAG 1 cut(s) 700
BfoI RGCGCY 1 cut(s) 225
BfuI GTATCC 1 cut(s) 653
BisI GCNGC 5 cut(s) 81, 255, 706, 1364, 1391
BlsI GCNGC 5 cut(s) 82, 256, 707, 1365, 1392
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 3 cut(s) 695, 1395, 1421
BmeRI GACNNNNNGTC 1 cut(s) 271
BmiI GGNNCC 3 cut(s) 79, 223, 753
BmrFI CCNGG 3 cut(s) 695, 1395, 1421
BmsI GCATC 4 cut(s) 76, 379, 1144, 1209
BpmI CTGGAG 1 cut(s) 1453
BpuEI CTTGAG 1 cut(s) 393
BpuMI CCSGG 3 cut(s) 695, 1395, 1421
BsaAI YACGTR 2 cut(s) 1057, 1072
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 566, 721, 1394
BsaWI WCCGGW 8 cut(s) 341, 530, 742, 962, 1118, 1138, 1168, 1381
BsaXI ACNNNNNCTCC 6 cut(s) 222, 252, 803, 833, 1041, 1071
Bsc4I CCNNNNNNNGG 6 cut(s) 330, 333, 452, 572, 795, 1476
Bse118I RCCGGY 4 cut(s) 20, 530, 742, 1168
Bse1I ACTGG 1 cut(s) 1264
BseAI TCCGGA 1 cut(s) 1138
BseDI CCNNGG 3 cut(s) 566, 721, 1394
BseGI GGATG 4 cut(s) 857, 1069, 1119, 1135
BseLI CCNNNNNNNGG 6 cut(s) 330, 333, 452, 572, 795, 1476
BseMII CTCAG 4 cut(s) 115, 189, 213, 1241
BseNI ACTGG 1 cut(s) 1264
BseRI GAGGAG 2 cut(s) 603, 1062
BseXI GCAGC 3 cut(s) 717, 1375, 1402
Bsh1236I CGCG 3 cut(s) 156, 264, 269
BshNI GGYRCC 2 cut(s) 221, 751
BshTI ACCGGT 3 cut(s) 530, 742, 1168
BsiWI CGTACG 1 cut(s) 450
BslFI GGGAC 2 cut(s) 556, 1411
BslI CCNNNNNNNGG 6 cut(s) 330, 333, 452, 572, 795, 1476
BsmAI GTCTC 3 cut(s) 225, 459, 823
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 2 cut(s) 556, 1411
BsmI GAATGC 1 cut(s) 631
Bsp13I TCCGGA 1 cut(s) 1138
Bsp143I GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
Bsp19I CCATGG 1 cut(s) 566
Bsp68I TCGCGA 1 cut(s) 264
BspACI CCGC 7 cut(s) 18, 81, 156, 254, 269, 324, 1361
BspCNI CTCAG 4 cut(s) 114, 188, 212, 1240
BspEI TCCGGA 1 cut(s) 1138
BspFNI CGCG 3 cut(s) 156, 264, 269
BspLI GGNNCC 3 cut(s) 79, 223, 753
BspPI GGATC 4 cut(s) 864, 895, 1148, 1421
BspT107I GGYRCC 2 cut(s) 221, 751
BsrFI RCCGGY 4 cut(s) 20, 530, 742, 1168
BsrI ACTGG 1 cut(s) 1264
BssAI RCCGGY 4 cut(s) 20, 530, 742, 1168
BssECI CCNNGG 3 cut(s) 566, 721, 1394
BssMI GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
BssNAI GTATAC 1 cut(s) 1069
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 1 cut(s) 793
BssT1I CCWWGG 2 cut(s) 566, 721
Bst1107I GTATAC 1 cut(s) 1069
Bst2BI CACGAG 1 cut(s) 793
Bst6I CTCTTC 3 cut(s) 156, 408, 451
BstACI GRCGYC 1 cut(s) 222
BstBAI YACGTR 2 cut(s) 1057, 1072
BstC8I GCNNGC 2 cut(s) 881, 1224
BstDEI CTNAG 6 cut(s) 101, 175, 199, 281, 1227, 1305
BstDSI CCRYGG 1 cut(s) 566
BstEII GGTNACC 1 cut(s) 23
BstF5I GGATG 4 cut(s) 857, 1069, 1119, 1135
BstFNI CGCG 3 cut(s) 156, 264, 269
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 8 cut(s) 115, 859, 903, 942, 1147, 1156, 1252, 1429
BstMAI GTCTC 3 cut(s) 225, 459, 823
BstMBI GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
BstMWI GCNNNNNNNGC 5 cut(s) 86, 135, 200, 941, 1232
BstNSI RCATGY 2 cut(s) 883, 918
BstPI GGTNACC 1 cut(s) 23
BstSCI CCNGG 3 cut(s) 693, 1393, 1419
BstSFI CTRYAG 1 cut(s) 700
BstSNI TACGTA 1 cut(s) 1072
BstUI CGCG 3 cut(s) 156, 264, 269
BstV1I GCAGC 3 cut(s) 717, 1375, 1402
BstX2I RGATCY 1 cut(s) 1426
BstYI RGATCY 1 cut(s) 1426
BstZ17I GTATAC 1 cut(s) 1069
BsuI GTATCC 1 cut(s) 653
BtgI CCRYGG 1 cut(s) 566
BtgZI GCGATG 2 cut(s) 110, 137
BtsCI GGATG 4 cut(s) 857, 1069, 1119, 1135
BtsI GCAGTG 2 cut(s) 440, 958
BtsIMutI CAGTG 4 cut(s) 108, 440, 759, 958
BtuMI TCGCGA 1 cut(s) 264
Cac8I GCNNGC 2 cut(s) 881, 1224
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 4 cut(s) 20, 530, 742, 1168
CseI GACGC 1 cut(s) 275
Csp6I GTAC 5 cut(s) 172, 451, 752, 786, 1326
CspAI ACCGGT 3 cut(s) 530, 742, 1168
CviAII CATG 6 cut(s) 95, 186, 567, 880, 915, 1198
CviQI GTAC 5 cut(s) 172, 451, 752, 786, 1326
DdeI CTNAG 6 cut(s) 101, 175, 199, 281, 1227, 1305
DinI GGCGCC 1 cut(s) 223
DpnI GATC 8 cut(s) 114, 858, 902, 941, 1146, 1155, 1251, 1428
DpnII GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
DriI GACNNNNNGTC 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 156, 408, 451
Eam1105I GACNNNNNGTC 1 cut(s) 271
EarI CTCTTC 3 cut(s) 156, 408, 451
Eco105I TACGTA 1 cut(s) 1072
Eco130I CCWWGG 2 cut(s) 566, 721
Eco57I CTGAAG 1 cut(s) 1008
Eco91I GGTNACC 1 cut(s) 23
EcoO65I GGTNACC 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 670
EcoT14I CCWWGG 2 cut(s) 566, 721
EcoT22I ATGCAT 2 cut(s) 633, 920
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 566, 721
Esp3I CGTCTC 1 cut(s) 225
FaeI CATG 6 cut(s) 98, 189, 570, 883, 918, 1201
FalI AAGNNNNNCTT 2 cut(s) 1437, 1469
FaqI GGGAC 2 cut(s) 556, 1411
FatI CATG 6 cut(s) 94, 185, 566, 879, 914, 1197
FauI CCCGC 1 cut(s) 331
FblI GTMKAC 3 cut(s) 493, 699, 1068
Fnu4HI GCNGC 5 cut(s) 81, 255, 706, 1364, 1391
FokI GGATG 4 cut(s) 864, 1076, 1122, 1126
Fsp4HI GCNGC 5 cut(s) 81, 255, 706, 1364, 1391
FspBI CTAG 1 cut(s) 1347
GlaI GCGC 1 cut(s) 223
GluI GCNGC 5 cut(s) 81, 255, 706, 1364, 1391
GsuI CTGGAG 1 cut(s) 1453
HaeII RGCGCY 1 cut(s) 225
HgaI GACGC 1 cut(s) 275
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 6 cut(s) 98, 189, 570, 883, 918, 1201
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HindIII AAGCTT 1 cut(s) 1451
HinfI GANTC 7 cut(s) 779, 867, 1013, 1020, 1174, 1357, 1432
HphI GGTGA 5 cut(s) 331, 520, 545, 683, 1183
Hpy166II GTNNAC 4 cut(s) 494, 700, 1069, 1257
Hpy188I TCNGA 9 cut(s) 77, 104, 637, 905, 1012, 1043, 1158, 1179, 1431
Hpy8I GTNNAC 4 cut(s) 494, 700, 1069, 1257
Hpy99I CGWCG 5 cut(s) 65, 138, 269, 830, 909
HpyAV CCTTC 8 cut(s) 38, 47, 53, 456, 474, 687, 1054, 1470
HpyCH4IV ACGT 5 cut(s) 1056, 1071, 1212, 1278, 1459
HpyCH4V TGCA 3 cut(s) 89, 631, 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 135, 200, 941, 1232
HpyF3I CTNAG 6 cut(s) 101, 175, 199, 281, 1227, 1305
HpySE526I ACGT 5 cut(s) 1056, 1071, 1212, 1278, 1459
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 6 cut(s) 98, 189, 570, 883, 918, 1201
HspAI GCGC 1 cut(s) 222
KasI GGCGCC 1 cut(s) 221
Kpn2I TCCGGA 1 cut(s) 1138
KpnI GGTACC 1 cut(s) 755
Kzo9I GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 3 cut(s) 717, 1375, 1402
LweI GCATC 4 cut(s) 76, 379, 1144, 1209
MaeI CTAG 1 cut(s) 1347
MaeII ACGT 5 cut(s) 1056, 1071, 1212, 1278, 1459
MalI GATC 8 cut(s) 114, 858, 902, 941, 1146, 1155, 1251, 1428
MboI GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
MboII GAAGA 9 cut(s) 23, 173, 425, 468, 986, 1001, 1309, 1436, 1485
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 1426
MluCI AATT 8 cut(s) 546, 578, 670, 969, 1186, 1263, 1321, 1414
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 1351
MmeI TCCRAC 1 cut(s) 928
Mph1103I ATGCAT 2 cut(s) 633, 920
MroI TCCGGA 1 cut(s) 1138
MroXI GAANNNNTTC 1 cut(s) 978
MseI TTAA 6 cut(s) 351, 512, 843, 1005, 1029, 1185
MslI CAYNNNNRTG 2 cut(s) 387, 636
MspA1I CMGCKG 1 cut(s) 254
MspR9I CCNGG 3 cut(s) 695, 1395, 1421
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 631
MvnI CGCG 3 cut(s) 156, 264, 269
MwoI GCNNNNNNNGC 5 cut(s) 86, 135, 200, 941, 1232
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 3 cut(s) 695, 1395, 1421
NcoI CCATGG 1 cut(s) 566
NdeII GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
NlaIII CATG 6 cut(s) 98, 189, 570, 883, 918, 1201
NlaIV GGNNCC 3 cut(s) 79, 223, 753
NmeAIII GCCGAG 2 cut(s) 927, 1231
NmuCI GTSAC 6 cut(s) 273, 419, 745, 1213, 1279, 1354
NruI TCGCGA 1 cut(s) 264
NsiI ATGCAT 2 cut(s) 633, 920
NspI RCATGY 2 cut(s) 883, 918
PaeI GCATGC 1 cut(s) 883
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 139, 1209, 1406
PctI GAATGC 1 cut(s) 631
PdmI GAANNNNTTC 1 cut(s) 978
PfeI GAWTC 6 cut(s) 779, 867, 1013, 1020, 1174, 1432
Pfl23II CGTACG 1 cut(s) 450
PflFI GACNNNGTC 1 cut(s) 1199
PfoI TCCNGGA 1 cut(s) 1419
PinAI ACCGGT 3 cut(s) 530, 742, 1168
PkrI GCNGC 5 cut(s) 82, 256, 707, 1365, 1392
PleI GAGTC 1 cut(s) 1351
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 1351
Ppu21I YACGTR 2 cut(s) 1057, 1072
PshBI ATTAAT 1 cut(s) 1185
Psp1406I AACGTT 1 cut(s) 1459
PspEI GGTNACC 1 cut(s) 23
PspLI CGTACG 1 cut(s) 450
PspN4I GGNNCC 3 cut(s) 79, 223, 753
PsuI RGATCY 1 cut(s) 1426
PsyI GACNNNGTC 1 cut(s) 1199
RruI TCGCGA 1 cut(s) 264
RsaI GTAC 5 cut(s) 173, 452, 753, 787, 1327
RsaNI GTAC 5 cut(s) 172, 451, 752, 786, 1326
RseI CAYNNNNRTG 2 cut(s) 387, 636
SaqAI TTAA 6 cut(s) 351, 512, 843, 1005, 1029, 1185
SatI GCNGC 5 cut(s) 81, 255, 706, 1364, 1391
Sau3AI GATC 8 cut(s) 112, 856, 900, 939, 1144, 1153, 1249, 1426
ScaI AGTACT 1 cut(s) 173
SchI GAGTC 1 cut(s) 1351
ScrFI CCNGG 3 cut(s) 695, 1395, 1421
SfaNI GCATC 4 cut(s) 76, 379, 1144, 1209
SfcI CTRYAG 1 cut(s) 700
SfoI GGCGCC 1 cut(s) 223
SgrAI CRCCGGYG 1 cut(s) 530
SmiMI CAYNNNNRTG 2 cut(s) 387, 636
SmlI CTYRAG 1 cut(s) 408
SmoI CTYRAG 1 cut(s) 408
SnaBI TACGTA 1 cut(s) 1072
SpeI ACTAGT 1 cut(s) 1346
SphI GCATGC 1 cut(s) 883
Sse9I AATT 8 cut(s) 546, 578, 670, 969, 1186, 1263, 1321, 1414
SsiI CCGC 7 cut(s) 18, 81, 156, 254, 269, 324, 1361
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 1 cut(s) 1347
StyD4I CCNGG 3 cut(s) 693, 1393, 1419
StyI CCWWGG 2 cut(s) 566, 721
TaiI ACGT 5 cut(s) 1059, 1074, 1215, 1281, 1462
TaqI TCGA 2 cut(s) 982, 1295
TasI AATT 8 cut(s) 546, 578, 670, 969, 1186, 1263, 1321, 1414
TatI WGTACW 2 cut(s) 171, 1325
TauI GCSGC 2 cut(s) 83, 257
TfiI GAWTC 6 cut(s) 779, 867, 1013, 1020, 1174, 1432
Tru1I TTAA 6 cut(s) 351, 512, 843, 1005, 1029, 1185
Tru9I TTAA 6 cut(s) 351, 512, 843, 1005, 1029, 1185
TscAI CASTG 4 cut(s) 115, 447, 766, 958
TseFI GTSAC 6 cut(s) 273, 419, 745, 1213, 1279, 1354
TseI GCWGC 3 cut(s) 705, 1363, 1390
Tsp45I GTSAC 6 cut(s) 273, 419, 745, 1213, 1279, 1354
TspDTI ATGAA 5 cut(s) 61, 441, 475, 1112, 1458
TspGWI ACGGA 2 cut(s) 468, 879
TspRI CASTG 4 cut(s) 115, 447, 766, 958
Tth111I GACNNNGTC 1 cut(s) 1199
VspI ATTAAT 1 cut(s) 1185
XapI RAATTY 3 cut(s) 578, 670, 1263
XceI RCATGY 2 cut(s) 883, 918
XmiI GTMKAC 3 cut(s) 493, 699, 1068
XmnI GAANNNNTTC 1 cut(s) 978
XspI CTAG 1 cut(s) 1347
ZrmI AGTACT 1 cut(s) 173
Zsp2I ATGCAT 2 cut(s) 633, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.