Rroxscaffold_3G00258950

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008948
Physical Location & Seq
Forward (+)
53362064 .. 53366743
4680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258950.1

Sequence Viewer

Length: 1755 bp
ATGCTCTCGGCCTCTGCTCCGGTCGTTTTGCGGAACCACCGCCGCTCCGATCTTTTTGCGGAACAACCACTCGTCATTGTCGCTCCGATTAGCCTTGTCGACGACGCAAAACCACCGGTCGCCATCGTTTTGCGAACCCAATCTGTCGGAGGTGCGGCCCGTGCGGCAATAGTCATGAGGCGGCGCCATGAATCGTGCCACCGAGACGACTTCATGCAACTCGGAGTCGTGCGGCTCCTTTTTGACTCTATATATACTTTGGTTCCGCAACAAACCAAAATACCATGCTTGAGAAACTACCTTTTGTGTGATGGGACTTTGGAGGACTCCCCAAAAGCTCAGTTACCTAAGATGGCTATGGTGGTACTACTGGTTCTGGTCTTATTGTGCACAAACGGAGTTGAAAGCCGGAAGGCTAAGAATGTGGACAGCTTTGAGTACTCAGCCATAAGTTGCAGAGCCCAAAGTGCATCACTGGCAGATTTTGGAGGAGTTGGTGATGGTATCACATCAAACACCAAGGCTTTTCAGGCAGCAATTCAGCATCTGAGTCAATATGAGTCACAAGGTGGATCCCAACTTTTTGTTCCTGCTGGGAAATGGTTAACTGGGAGCTTCAACCTCACCAGCCATTTCACTCTCTATCTCCACAAGGATGCTGTTCTTCTTGCCTCTCAGGATGAGAATGAATGGCCAGTGATTGATCCACTTCCATCGTACGGTAGAGGAAGGGATACTGCTGGAGGAAGATTCATCAGTCTGATATTTGGAACCAACCTCACTGATGTTGTAGTAACAGGGGAAAATGGCACAATTGATGGACAAGGTGAACTATGGTGGCAAAAATTCCACAAGGGGGAGTTAAAGTACACTCGGCCTTACCTGATTGAAATCATGTTCTCAGAAAATATCCAAATTTCGAACCTTACTTTGGTTAATTCTCCATCATGGAATGTTCATCCTGTTTACAGCAGCAATGTTCTAGTTCAAGGCCTTACAATCCTTGCACCAGTAACGTCTCCAAACACAGATGGGATCAACCCCGACTCTTGCACAAATACTCGAATTGAGGACTGTTACATTGTCTCTGGAGATGATTGTATAGCCGTAAAAAGTGGTTGGGATGAGTATGGTATTGCTTTTGGGATGCCAACCAAAGCGCTTGTGATCAGACGTTTGACATGCATTTCTCCATTTAGTGCTGTGATTGCACTCGGAAGTGAGATGTCTGGTGGGATCCAAGATGTCAGGGCTGAGGATATCCTTGCCATTAACACGGAATCAGGAGTCAGAATCAAAACTGCTGTAGGAAGAGGAGGCTTTGTGAAAGACATATATGTCAGAGGGATGACCATGAAAACCATGAAGTATGTATTTTGGATTGCAGGAGACTATGGATCACATGCAGATGATGGCTATGATCCTAATGCGCTTCCAGTGATCCAGAACATAAATTATCATGATATGGTGGCTGAGAATGTAACAATGGCAGGTAAACTGGGGGGAATAACTGGAGATCCATTTACTGGGATTTGCATATCTAATGTTACAATTACAATGGCAAAGAAGGGAAAGAAAGTACCTTGGAACTGCACTGACGTTGCTGGGATTTCAAGCAGAGTGGTTCCTCAGCCGTGTGAGCTTCTAGCAGACCAGGGTCCGGAGAAGATCGGGGCATGTGATTTCCCAGAAGAAAGTCTTCCGGTTGATAATATGCAACTTCAGCTCTGCTCTTACAGAGGAAAGTACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

584

Amino Acids

63.59

Weight (kDa)

5.75

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 254 - 534 1.1e-38 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1337
Acc36I ACCTGC 1 cut(s) 1481
AccB1I GGYRCC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 1526
AccBSI CCGCTC 1 cut(s) 45
AccI GTMKAC 1 cut(s) 99
AccIII TCCGGA 1 cut(s) 1660
AciI CCGC 9 cut(s) 31, 40, 43, 59, 155, 164, 181, 232, 266
AcoI YGGCCR 1 cut(s) 692
AcsI RAATTY 2 cut(s) 845, 915
AcuI CTGAAG 1 cut(s) 1706
AcyI GRCGYC 1 cut(s) 184
AdeI CACNNNGTG 1 cut(s) 569
AfaI GTAC 6 cut(s) 366, 440, 719, 869, 1581, 1748
AfeI AGCGCT 1 cut(s) 1161
AfiI CCNNNNNNNGG 5 cut(s) 719, 856, 931, 1526, 1660
AgeI ACCGGT 1 cut(s) 115
AgsI TTSAA 5 cut(s) 404, 619, 890, 989, 1614
AjnI CCWGG 1 cut(s) 1653
AjuI GAANNNNNNNTTGG 4 cut(s) 570, 602, 914, 946
AluBI AGCT 5 cut(s) 338, 432, 615, 1642, 1726
AluI AGCT 5 cut(s) 338, 432, 615, 1642, 1726
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 4 cut(s) 198, 1023, 1090, 1383
Alw44I GTGCAC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 547
Aor13HI TCCGGA 1 cut(s) 1660
Aor51HI AGCGCT 1 cut(s) 1161
AoxI GGCC 5 cut(s) 9, 156, 692, 875, 991
ApaLI GTGCAC 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 533, 972
ApoI RAATTY 2 cut(s) 845, 915
AsiGI ACCGGT 1 cut(s) 115
Asp700I GAANNNNTTC 1 cut(s) 1698
AspLEI GCGC 3 cut(s) 186, 1162, 1432
AspS9I GGNCC 2 cut(s) 157, 1658
AsuHPI GGTGA 3 cut(s) 509, 616, 839
AsuII TTCGAA 1 cut(s) 920
AvaII GGWCC 1 cut(s) 1658
BaeGI GKGCMC 1 cut(s) 392
BalI TGGCCA 1 cut(s) 694
BamHI GGATCC 2 cut(s) 572, 1236
BanI GGYRCC 1 cut(s) 183
BanII GRGCYC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 1691
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 2 cut(s) 1254, 1629
BbvI GCAGC 2 cut(s) 545, 984
BccI CCATC 9 cut(s) 131, 305, 346, 494, 721, 812, 952, 1025, 1406
BceAI ACGGC 2 cut(s) 1091, 1618
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 1 cut(s) 1655
BciVI GTATCC 1 cut(s) 727
BclI TGATCA 1 cut(s) 1167
BcoDI GTCTC 4 cut(s) 198, 1023, 1090, 1383
BfaI CTAG 2 cut(s) 983, 1646
BfmI CTRYAG 1 cut(s) 1305
BfoI RGCGCY 2 cut(s) 187, 1163
BfuAI ACCTGC 1 cut(s) 1481
BfuI GTATCC 1 cut(s) 727
BglI GCCNNNNNGGC 1 cut(s) 164
BisI GCNGC 7 cut(s) 43, 156, 165, 182, 233, 534, 973
BlsI GCNGC 7 cut(s) 44, 157, 166, 183, 234, 535, 974
BmcAI AGTACT 2 cut(s) 440, 1748
Bme1390I CCNGG 1 cut(s) 1655
Bme18I GGWCC 1 cut(s) 1658
BmgT120I GGNCC 2 cut(s) 157, 1658
BmiI GGNNCC 9 cut(s) 35, 185, 236, 264, 574, 772, 1238, 1626, 1659
BmrFI CCNGG 1 cut(s) 1655
BmrI ACTGGG 3 cut(s) 618, 1508, 1536
BmsI GCATC 4 cut(s) 479, 553, 646, 1137
BmuI ACTGGG 3 cut(s) 618, 1508, 1536
BoxI GACNNNNGTC 1 cut(s) 1656
BpiI GAAGAC 1 cut(s) 1691
BpmI CTGGAG 3 cut(s) 762, 1110, 1533
Bpu10I CCTNAGC 2 cut(s) 1254, 1629
Bpu14I TTCGAA 1 cut(s) 920
BpuEI CTTGAG 1 cut(s) 310
BsaBI GATNNNNATC 1 cut(s) 890
BsaHI GRCGYC 1 cut(s) 184
BsaJI CCNNGG 3 cut(s) 519, 1583, 1654
BsaWI WCCGGW 4 cut(s) 19, 115, 1660, 1702
BsaXI ACNNNNNCTCC 6 cut(s) 29, 59, 851, 881, 1308, 1338
Bsc4I CCNNNNNNNGG 5 cut(s) 719, 856, 931, 1526, 1660
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 9 cut(s) 375, 480, 613, 695, 1010, 1436, 1503, 1516, 1531
Bse3DI GCAATG 1 cut(s) 982
Bse8I GATNNNNATC 1 cut(s) 890
BseAI TCCGGA 1 cut(s) 1660
BseBI CCWGG 1 cut(s) 1655
BseDI CCNNGG 3 cut(s) 519, 1583, 1654
BseGI GGATG 6 cut(s) 661, 685, 958, 1129, 1152, 1353
BseJI GATNNNNATC 1 cut(s) 890
BseLI CCNNNNNNNGG 5 cut(s) 719, 856, 931, 1526, 1660
BseMI GCAATG 1 cut(s) 982
BseMII CTCAG 8 cut(s) 353, 456, 539, 689, 915, 1245, 1464, 1643
BseNI ACTGG 9 cut(s) 375, 480, 613, 695, 1010, 1436, 1503, 1516, 1531
BseRI GAGGAG 2 cut(s) 504, 1329
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 2 cut(s) 545, 984
BseYI CCCAGC 2 cut(s) 593, 1604
BsgI GTGCAG 1 cut(s) 1576
Bsh1285I CGRYCG 2 cut(s) 24, 120
BshFI GGCC 5 cut(s) 11, 158, 694, 877, 993
BshNI GGYRCC 1 cut(s) 183
BshTI ACCGGT 1 cut(s) 115
BsiEI CGRYCG 2 cut(s) 24, 120
BsiHKAI GWGCWC 1 cut(s) 392
BsiSI CCGG 5 cut(s) 20, 116, 409, 1661, 1703
BsiWI CGTACG 1 cut(s) 717
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 5 cut(s) 719, 856, 931, 1526, 1660
BsmAI GTCTC 4 cut(s) 198, 1023, 1090, 1383
BsmBI CGTCTC 2 cut(s) 198, 1023
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 5 cut(s) 11, 158, 694, 877, 993
Bsp119I TTCGAA 1 cut(s) 920
Bsp1286I GDGCHC 2 cut(s) 392, 463
Bsp13I TCCGGA 1 cut(s) 1660
BspACI CCGC 9 cut(s) 31, 40, 43, 59, 155, 164, 181, 232, 266
BspANI GGCC 5 cut(s) 11, 158, 694, 877, 993
BspCNI CTCAG 8 cut(s) 352, 455, 540, 688, 914, 1246, 1465, 1642
BspEI TCCGGA 1 cut(s) 1660
BspHI TCATGA 2 cut(s) 174, 1459
BspLI GGNNCC 9 cut(s) 35, 185, 236, 264, 574, 772, 1238, 1626, 1659
BspMI ACCTGC 1 cut(s) 1481
BspT104I TTCGAA 1 cut(s) 920
BspT107I GGYRCC 1 cut(s) 183
BsrBI CCGCTC 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 982
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 9 cut(s) 375, 480, 613, 695, 1010, 1436, 1503, 1516, 1531
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 519, 1583, 1654
BssNI GRCGYC 1 cut(s) 184
BssT1I CCWWGG 2 cut(s) 519, 1583
Bst2UI CCWGG 1 cut(s) 1655
Bst4CI ACNGT 2 cut(s) 722, 1076
Bst6I CTCTTC 1 cut(s) 1306
BstACI GRCGYC 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 920
BstF5I GGATG 6 cut(s) 661, 685, 958, 1129, 1152, 1353
BstH2I RGCGCY 2 cut(s) 187, 1163
BstHHI GCGC 3 cut(s) 186, 1162, 1432
BstMAI GTCTC 4 cut(s) 198, 1023, 1090, 1383
BstMCI CGRYCG 2 cut(s) 24, 120
BstMWI GCNNNNNNNGC 8 cut(s) 161, 164, 467, 476, 530, 1208, 1639, 1723
BstNI CCWGG 1 cut(s) 1655
BstNSI RCATGY 3 cut(s) 1185, 1406, 1680
BstPAI GACNNNNGTC 1 cut(s) 1656
BstSCI CCNGG 1 cut(s) 1653
BstSFI CTRYAG 1 cut(s) 1305
BstSLI GKGCMC 1 cut(s) 392
BstV1I GCAGC 2 cut(s) 545, 984
BstV2I GAAGAC 1 cut(s) 1691
BstX2I RGATCY 3 cut(s) 572, 1236, 1516
BstYI RGATCY 3 cut(s) 572, 1236, 1516
BsuI GTATCC 1 cut(s) 727
BsuRI GGCC 5 cut(s) 11, 158, 694, 877, 993
BtsCI GGATG 6 cut(s) 661, 685, 958, 1129, 1152, 1353
BtsIMutI CAGTG 5 cut(s) 473, 702, 780, 1443, 1593
BveI ACCTGC 1 cut(s) 1481
CaiI CAGNNNCTG 1 cut(s) 547
CciI TCATGA 2 cut(s) 174, 1459
CfoI GCGC 3 cut(s) 186, 1162, 1432
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 2 cut(s) 157, 1658
CseI GACGC 1 cut(s) 113
Csp6I GTAC 6 cut(s) 365, 439, 718, 868, 1580, 1747
CspAI ACCGGT 1 cut(s) 115
CspCI CAANNNNNGTGG 2 cut(s) 1602, 1637
CviQI GTAC 6 cut(s) 365, 439, 718, 868, 1580, 1747
DinI GGCGCC 1 cut(s) 185
DraIII CACNNNGTG 1 cut(s) 569
DrdI GACNNNNNNGTC 1 cut(s) 1337
DseDI GACNNNNNNGTC 1 cut(s) 1337
EaeI YGGCCR 1 cut(s) 692
Eam1104I CTCTTC 1 cut(s) 1306
EarI CTCTTC 1 cut(s) 1306
Eco130I CCWWGG 2 cut(s) 519, 1583
Eco147I AGGCCT 1 cut(s) 993
Eco24I GRGCYC 1 cut(s) 463
Eco32I GATATC 1 cut(s) 1261
Eco47I GGWCC 1 cut(s) 1658
Eco47III AGCGCT 1 cut(s) 1161
Eco57I CTGAAG 1 cut(s) 1706
EcoRII CCWGG 1 cut(s) 1653
EcoRV GATATC 1 cut(s) 1261
EcoT14I CCWWGG 2 cut(s) 519, 1583
EcoT22I ATGCAT 1 cut(s) 1187
EcoT38I GRGCYC 1 cut(s) 463
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
ErhI CCWWGG 2 cut(s) 519, 1583
Esp3I CGTCTC 2 cut(s) 198, 1023
FalI AAGNNNNNCTT 2 cut(s) 1683, 1715
FaqI GGGAC 1 cut(s) 328
FbaI TGATCA 1 cut(s) 1167
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 7 cut(s) 43, 156, 165, 182, 233, 534, 973
FokI GGATG 6 cut(s) 668, 692, 945, 1136, 1159, 1360
FriOI GRGCYC 1 cut(s) 463
Fsp4HI GCNGC 7 cut(s) 43, 156, 165, 182, 233, 534, 973
FspBI CTAG 2 cut(s) 983, 1646
GlaI GCGC 3 cut(s) 185, 1161, 1431
GluI GCNGC 7 cut(s) 43, 156, 165, 182, 233, 534, 973
GsaI CCCAGC 2 cut(s) 597, 1608
GsuI CTGGAG 3 cut(s) 762, 1110, 1533
HaeII RGCGCY 2 cut(s) 187, 1163
HaeIII GGCC 5 cut(s) 11, 158, 694, 877, 993
HapII CCGG 5 cut(s) 20, 116, 409, 1661, 1703
HgaI GACGC 1 cut(s) 113
HhaI GCGC 3 cut(s) 186, 1162, 1432
Hin1I GRCGYC 1 cut(s) 184
Hin6I GCGC 3 cut(s) 184, 1160, 1430
HinP1I GCGC 3 cut(s) 184, 1160, 1430
HincII GTYRAC 2 cut(s) 100, 606
HindII GTYRAC 2 cut(s) 100, 606
HpaI GTTAAC 1 cut(s) 606
HpaII CCGG 5 cut(s) 20, 116, 409, 1661, 1703
HphI GGTGA 3 cut(s) 509, 616, 839
Hpy166II GTNNAC 8 cut(s) 100, 390, 427, 606, 830, 870, 967, 1496
Hpy188III TCNNGA 7 cut(s) 175, 677, 1089, 1284, 1444, 1460, 1661
Hpy8I GTNNAC 8 cut(s) 100, 390, 427, 606, 830, 870, 967, 1496
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 3 cut(s) 406, 723, 1561
HpyCH4III ACNGT 2 cut(s) 722, 1076
HpyCH4IV ACGT 3 cut(s) 1016, 1174, 1599
HpyF10VI GCNNNNNNNGC 8 cut(s) 161, 164, 467, 476, 530, 1208, 1639, 1723
HpySE526I ACGT 3 cut(s) 1016, 1174, 1599
Hsp92I GRCGYC 1 cut(s) 184
HspAI GCGC 3 cut(s) 184, 1160, 1430
KasI GGCGCC 1 cut(s) 183
Kpn2I TCCGGA 1 cut(s) 1660
Ksp22I TGATCA 1 cut(s) 1167
KspAI GTTAAC 1 cut(s) 606
LmnI GCTCC 5 cut(s) 22, 50, 88, 240, 612
Lsp1109I GCAGC 2 cut(s) 545, 984
LweI GCATC 4 cut(s) 479, 553, 646, 1137
MaeI CTAG 2 cut(s) 983, 1646
MaeII ACGT 3 cut(s) 1016, 1174, 1599
MaeIII GTNAC 7 cut(s) 342, 561, 793, 1012, 1076, 1480, 1546
MbiI CCGCTC 1 cut(s) 45
MboII GAAGA 6 cut(s) 656, 759, 1323, 1678, 1691, 1703
MfeI CAATTG 1 cut(s) 813
MflI RGATCY 3 cut(s) 572, 1236, 1516
MhlI GDGCHC 2 cut(s) 392, 463
MlsI TGGCCA 1 cut(s) 694
MluCI AATT 8 cut(s) 537, 813, 845, 915, 937, 1065, 1453, 1551
MluNI TGGCCA 1 cut(s) 694
Mly113I GGCGCC 1 cut(s) 184
MlyI GAGTC 7 cut(s) 234, 239, 320, 559, 569, 1040, 1296
MmeI TCCRAC 1 cut(s) 127
Mox20I TGGCCA 1 cut(s) 694
Mph1103I ATGCAT 1 cut(s) 1187
MroI TCCGGA 1 cut(s) 1660
MroXI GAANNNNTTC 1 cut(s) 1698
MscI TGGCCA 1 cut(s) 694
MseI TTAA 4 cut(s) 605, 863, 936, 1272
MslI CAYNNNNRTG 2 cut(s) 654, 1407
Msp20I TGGCCA 1 cut(s) 694
MspI CCGG 5 cut(s) 20, 116, 409, 1661, 1703
MspR9I CCNGG 1 cut(s) 1655
MunI CAATTG 1 cut(s) 813
MvaI CCWGG 1 cut(s) 1655
MwoI GCNNNNNNNGC 8 cut(s) 161, 164, 467, 476, 530, 1208, 1639, 1723
NarI GGCGCC 1 cut(s) 184
NlaIV GGNNCC 9 cut(s) 35, 185, 236, 264, 574, 772, 1238, 1626, 1659
NmeAIII GCCGAG 1 cut(s) 853
NmuCI GTSAC 1 cut(s) 561
NsiI ATGCAT 1 cut(s) 1187
NspI RCATGY 3 cut(s) 1185, 1406, 1680
NspV TTCGAA 1 cut(s) 920
PagI TCATGA 2 cut(s) 174, 1459
PceI AGGCCT 1 cut(s) 993
PdmI GAANNNNTTC 1 cut(s) 1698
PfeI GAWTC 4 cut(s) 191, 750, 1280, 1293
Pfl23II CGTACG 1 cut(s) 717
PflMI CCANNNNNTGG 1 cut(s) 1526
PinAI ACCGGT 1 cut(s) 115
PkrI GCNGC 7 cut(s) 44, 157, 166, 183, 234, 535, 974
PleI GAGTC 7 cut(s) 233, 239, 320, 558, 568, 1040, 1295
PluTI GGCGCC 1 cut(s) 187
PpsI GAGTC 7 cut(s) 233, 239, 320, 558, 568, 1040, 1295
PshAI GACNNNNGTC 1 cut(s) 1656
Psp6I CCWGG 1 cut(s) 1653
PspFI CCCAGC 2 cut(s) 593, 1604
PspGI CCWGG 1 cut(s) 1653
PspLI CGTACG 1 cut(s) 717
PspN4I GGNNCC 9 cut(s) 35, 185, 236, 264, 574, 772, 1238, 1626, 1659
PspPI GGNCC 2 cut(s) 157, 1658
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PstNI CAGNNNCTG 1 cut(s) 547
PsuI RGATCY 3 cut(s) 572, 1236, 1516
RsaI GTAC 6 cut(s) 366, 440, 719, 869, 1581, 1748
RsaNI GTAC 6 cut(s) 365, 439, 718, 868, 1580, 1747
RseI CAYNNNNRTG 2 cut(s) 654, 1407
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 4 cut(s) 605, 863, 936, 1272
SatI GCNGC 7 cut(s) 43, 156, 165, 182, 233, 534, 973
Sau96I GGNCC 2 cut(s) 157, 1658
ScaI AGTACT 2 cut(s) 440, 1748
SchI GAGTC 7 cut(s) 234, 239, 320, 559, 569, 1040, 1296
ScrFI CCNGG 1 cut(s) 1655
SduI GDGCHC 2 cut(s) 392, 463
SfaNI GCATC 4 cut(s) 479, 553, 646, 1137
SfcI CTRYAG 1 cut(s) 1305
SfoI GGCGCC 1 cut(s) 185
SfuI TTCGAA 1 cut(s) 920
SinI GGWCC 1 cut(s) 1658
SmiMI CAYNNNNRTG 2 cut(s) 654, 1407
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 8 cut(s) 537, 813, 845, 915, 937, 1065, 1453, 1551
SseBI AGGCCT 1 cut(s) 993
SsiI CCGC 9 cut(s) 31, 40, 43, 59, 155, 164, 181, 232, 266
SspDI GGCGCC 1 cut(s) 183
SspMI CTAG 2 cut(s) 983, 1646
StuI AGGCCT 1 cut(s) 993
StyD4I CCNGG 1 cut(s) 1653
StyI CCWWGG 2 cut(s) 519, 1583
TaaI ACNGT 2 cut(s) 722, 1076
TaiI ACGT 3 cut(s) 1019, 1177, 1602
TaqI TCGA 3 cut(s) 99, 920, 1063
TasI AATT 8 cut(s) 537, 813, 845, 915, 937, 1065, 1453, 1551
TatI WGTACW 3 cut(s) 438, 867, 1746
TauI GCSGC 5 cut(s) 45, 158, 167, 184, 235
TfiI GAWTC 4 cut(s) 191, 750, 1280, 1293
Tru1I TTAA 4 cut(s) 605, 863, 936, 1272
Tru9I TTAA 4 cut(s) 605, 863, 936, 1272
TscAI CASTG 5 cut(s) 480, 702, 787, 1443, 1600
TseFI GTSAC 1 cut(s) 561
TseI GCWGC 2 cut(s) 533, 972
Tsp45I GTSAC 1 cut(s) 561
TspDTI ATGAA 7 cut(s) 202, 204, 702, 742, 947, 1370, 1379
TspGWI ACGGA 2 cut(s) 411, 1292
TspRI CASTG 5 cut(s) 480, 702, 787, 1443, 1600
Van91I CCANNNNNTGG 1 cut(s) 1526
VneI GTGCAC 1 cut(s) 388
VpaK11BI GGWCC 1 cut(s) 1658
XapI RAATTY 2 cut(s) 845, 915
XceI RCATGY 3 cut(s) 1185, 1406, 1680
XmiI GTMKAC 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 1698
XspI CTAG 2 cut(s) 983, 1646
ZrmI AGTACT 2 cut(s) 440, 1748
Zsp2I ATGCAT 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.