FvH4_5g03040

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008948
Physical Location & Seq
Forward (+)
1823143 .. 1826739
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03040.t3

Sequence Viewer

Length: 1527 bp
ATGAAGCTAAAGTTAATGACTATATATACTTTGGTCTGCACCAAACCAATATACCAGGTTTCAGAAACTACTTTGTGTATGATGGGATTTTGGAATACTCCCCAAAAAGCTCAGTTACCTACGATGGCTGTGGTAGTACTACTGGTTCTGGTTTTCTTATGCACAAACGGAGTTGAAAGCCGGAAGGCTAAACATGTGGACTGTTTTCAGTACTCAGCCATAAGTTGCAGAGCCTACGGTGCATCACTGGCAGATTTTGGAGGAGTTGGTGATGGTACCACATCAAACACCAAGGCCTTTCAGGCAGCAATTCAGCATCTGAGTCAATATGAATCACAAGGTGGATCCCAACTTTTTGTTCCTGCTGGGAAATGGTTAACTGGGAGCTTCAACCTCACCAGCCATTTCACTCTCTATCTCCACAAGGATGCTGTACTTCTTGCCTCTCAGGATGAGAATGAATGGCCTGTGATTGATCCACTTCCATCGTACGGTCGAGGGAGGGATACAGCTGGAGGAAGATTCATCAGTCTGATATTTGGAACCAACCTCACTGATGTTGTAGTAACAGGGGAAAATGGCACAATTGATGGTCAAGGTGAACTATGGTGGCAAAAATTCCGCAAGGGGGAGTTAAAGTACACTCGGCCTTACCTGATCGAAATCATGTTCTCAGAGAATATCCAGATTTCCAACCTCACTTTCATCAATTCTCCATCATGGAATGTCCATCCTGTTTACAGCAGCAATGTTCTTGTTCAAGGCCTTACAATCCTTGCACCAGTGACATCTCCAAATACAGATGGAATCAACCCTGACTCTTGCACTAATACTCGAATTGAGGACTGTTACATTGTTTCTGGAGATGATTGCATAGCCGTGAAGAGTGGTTGGGATGAGTATGGTATTGCTTTTGGGATGCCAACCAAAGCACTTGTGATCAGACGTTTGACATGCATTTCTCCATTTAGTGCGGTGATTGCACTCGGAAGCGAGATGTCAGGCGGGATCCAAGATGTTAGGGCTGAGGATATCTTTGCCATCAATACGGAATCAGCAGTCAGAATCAAAACTGCTGTAGGAAGAGGAGGTTTTGTGAAAGACATATATGTTAGAAGGATGACCATGCATACCATGAAGTATGTATTTTGGATTGCAGGAGATTATGGATCGCATGCAGATAATGGTTATGATCCTAATGCACTTCCGGTGATTCAGAACATAAATTATCATGATATGGTAGCTGAGAATGTAACAATGGCTGGTAAGCTAGGGGGATTAACTGGTGATCCATTTACCGGGATTTGCATATCTAATGTTACAATAACAATGGCAAAGAAGGGAAAGAAAGTACCTTGGAACTGCACTGATGTTGCTGGGATTTCAAGTGGAGTGGTTCCTCAGCCGTGTGAGCTTCTGGCAGACCAGGGTCCGGAGAAGATAGGGGCATGTAACTTCCCAGAAGAAAGTCTGCCAGTTGATAATATGCAAGTTCAGCTGTGCACTTACAGAGGAAACTACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

509

Amino Acids

55.5

Weight (kDa)

5.68

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 173 - 453 6.6e-38 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 275
AccIII TCCGGA 1 cut(s) 1432
AciI CCGC 3 cut(s) 622, 974, 1005
AclWI GGATC 8 cut(s) 339, 352, 470, 1003, 1016, 1177, 1187, 1283
AcsI RAATTY 1 cut(s) 617
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 7 cut(s) 138, 212, 277, 435, 491, 641, 1353
AfiI CCNNNNNNNGG 4 cut(s) 491, 628, 1298, 1432
AflIII ACRYGT 1 cut(s) 193
AgsI TTSAA 4 cut(s) 176, 391, 761, 1386
AjnI CCWGG 2 cut(s) 54, 1425
AjuI GAANNNNNNNTTGG 4 cut(s) 342, 374, 686, 718
AluBI AGCT 8 cut(s) 7, 110, 387, 512, 1244, 1270, 1414, 1498
AluI AGCT 8 cut(s) 7, 110, 387, 512, 1244, 1270, 1414, 1498
Alw21I GWGCWC 1 cut(s) 1505
Alw44I GTGCAC 1 cut(s) 1501
AlwI GGATC 8 cut(s) 339, 352, 470, 1003, 1016, 1177, 1187, 1283
AlwNI CAGNNNCTG 1 cut(s) 319
Aor13HI TCCGGA 1 cut(s) 1432
AoxI GGCC 4 cut(s) 294, 464, 647, 763
ApaLI GTGCAC 1 cut(s) 1501
ApeKI GCWGC 2 cut(s) 305, 744
ApoI RAATTY 1 cut(s) 617
Asp718I GGTACC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 1430
AsuC2I CCSGG 1 cut(s) 1300
AsuHPI GGTGA 6 cut(s) 281, 388, 611, 988, 1222, 1298
AvaII GGWCC 1 cut(s) 1430
BaeGI GKGCMC 1 cut(s) 1505
BamHI GGATCC 2 cut(s) 344, 1008
BanI GGYRCC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 1505
BbvCI CCTCAGC 2 cut(s) 1026, 1401
BbvI GCAGC 2 cut(s) 317, 756
BccI CCATC 9 cut(s) 76, 118, 266, 493, 584, 724, 738, 797, 1049
BceAI ACGGC 2 cut(s) 863, 1390
BciT130I CCWGG 2 cut(s) 56, 1427
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 1 cut(s) 939
BcnI CCSGG 1 cut(s) 1300
BfaI CTAG 1 cut(s) 1271
BfmI CTRYAG 1 cut(s) 1077
BfuI GTATCC 1 cut(s) 499
BglI GCCNNNNNGGC 1 cut(s) 302
BisI GCNGC 2 cut(s) 306, 745
BlsI GCNGC 2 cut(s) 307, 746
BmcAI AGTACT 2 cut(s) 138, 212
Bme1390I CCNGG 3 cut(s) 56, 1300, 1427
Bme18I GGWCC 1 cut(s) 1430
BmgT120I GGNCC 1 cut(s) 1430
BmiI GGNNCC 6 cut(s) 277, 346, 544, 1010, 1398, 1431
BmrFI CCNGG 3 cut(s) 56, 1300, 1427
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 4 cut(s) 251, 325, 418, 909
BmuI ACTGGG 1 cut(s) 390
BoxI GACNNNNGTC 1 cut(s) 1428
BpmI CTGGAG 2 cut(s) 534, 882
Bpu10I CCTNAGC 2 cut(s) 1026, 1401
BpuMI CCSGG 1 cut(s) 1300
BsaBI GATNNNNATC 1 cut(s) 662
BsaJI CCNNGG 3 cut(s) 291, 1355, 1426
BsaWI WCCGGW 2 cut(s) 1207, 1432
BsaXI ACNNNNNCTCC 4 cut(s) 623, 653, 1080, 1110
Bsc4I CCNNNNNNNGG 4 cut(s) 491, 628, 1298, 1432
Bse1I ACTGG 6 cut(s) 147, 252, 385, 782, 1288, 1475
Bse3DI GCAATG 1 cut(s) 754
Bse8I GATNNNNATC 1 cut(s) 662
BseAI TCCGGA 1 cut(s) 1432
BseBI CCWGG 2 cut(s) 56, 1427
BseDI CCNNGG 3 cut(s) 291, 1355, 1426
BseGI GGATG 6 cut(s) 433, 457, 730, 901, 924, 1125
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 4 cut(s) 491, 628, 1298, 1432
BseMI GCAATG 1 cut(s) 754
BseMII CTCAG 8 cut(s) 125, 228, 311, 461, 687, 1017, 1236, 1415
BseNI ACTGG 6 cut(s) 147, 252, 385, 782, 1288, 1475
BseRI GAGGAG 2 cut(s) 276, 1101
BseSI GKGCMC 1 cut(s) 1505
BseXI GCAGC 2 cut(s) 317, 756
BseYI CCCAGC 2 cut(s) 365, 1376
BsgI GTGCAG 2 cut(s) 22, 1348
Bsh1285I CGRYCG 1 cut(s) 496
BshFI GGCC 4 cut(s) 296, 466, 649, 765
BshNI GGYRCC 1 cut(s) 275
BsiEI CGRYCG 1 cut(s) 496
BsiHKAI GWGCWC 1 cut(s) 1505
BsiSI CCGG 4 cut(s) 181, 1208, 1299, 1433
BsiWI CGTACG 1 cut(s) 489
BslI CCNNNNNNNGG 4 cut(s) 491, 628, 1298, 1432
BsnI GGCC 4 cut(s) 296, 466, 649, 765
Bsp1286I GDGCHC 1 cut(s) 1505
Bsp13I TCCGGA 1 cut(s) 1432
Bsp143I GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
BspACI CCGC 3 cut(s) 622, 974, 1005
BspANI GGCC 4 cut(s) 296, 466, 649, 765
BspCNI CTCAG 8 cut(s) 124, 227, 312, 460, 686, 1018, 1237, 1414
BspEI TCCGGA 1 cut(s) 1432
BspHI TCATGA 1 cut(s) 1231
BspLI GGNNCC 6 cut(s) 277, 346, 544, 1010, 1398, 1431
BspPI GGATC 8 cut(s) 339, 352, 470, 1003, 1016, 1177, 1187, 1283
BspT107I GGYRCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 754
BsrI ACTGG 6 cut(s) 147, 252, 385, 782, 1288, 1475
BssECI CCNNGG 3 cut(s) 291, 1355, 1426
BssMI GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
BssT1I CCWWGG 2 cut(s) 291, 1355
Bst2UI CCWGG 2 cut(s) 56, 1427
Bst4CI ACNGT 4 cut(s) 203, 239, 494, 848
Bst6I CTCTTC 2 cut(s) 878, 1078
BstC8I GCNNGC 1 cut(s) 1176
BstDEI CTNAG 8 cut(s) 111, 214, 320, 447, 673, 1026, 1245, 1401
BstF5I GGATG 6 cut(s) 433, 457, 730, 901, 924, 1125
BstKTI GATC 8 cut(s) 347, 478, 660, 942, 1011, 1172, 1195, 1291
BstMBI GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
BstMCI CGRYCG 1 cut(s) 496
BstMWI GCNNNNNNNGC 6 cut(s) 239, 248, 302, 980, 1411, 1495
BstNI CCWGG 2 cut(s) 56, 1427
BstNSI RCATGY 4 cut(s) 197, 957, 1178, 1452
BstPAI GACNNNNGTC 1 cut(s) 1428
BstSCI CCNGG 3 cut(s) 54, 1298, 1425
BstSFI CTRYAG 1 cut(s) 1077
BstSLI GKGCMC 1 cut(s) 1505
BstV1I GCAGC 2 cut(s) 317, 756
BstX2I RGATCY 2 cut(s) 344, 1008
BstYI RGATCY 2 cut(s) 344, 1008
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 4 cut(s) 296, 466, 649, 765
BtsCI GGATG 6 cut(s) 433, 457, 730, 901, 924, 1125
BtsIMutI CAGTG 4 cut(s) 245, 552, 789, 1365
Cac8I GCNNGC 1 cut(s) 1176
CaiI CAGNNNCTG 1 cut(s) 319
CciI TCATGA 1 cut(s) 1231
Cfr13I GGNCC 1 cut(s) 1430
CsiI ACCWGGT 1 cut(s) 54
Csp6I GTAC 7 cut(s) 137, 211, 276, 434, 490, 640, 1352
CspCI CAANNNNNGTGG 2 cut(s) 1374, 1409
CviAII CATG 9 cut(s) 194, 667, 720, 954, 1126, 1135, 1175, 1232, 1449
CviQI GTAC 7 cut(s) 137, 211, 276, 434, 490, 640, 1352
DdeI CTNAG 8 cut(s) 111, 214, 320, 447, 673, 1026, 1245, 1401
DpnI GATC 8 cut(s) 346, 477, 659, 941, 1010, 1171, 1194, 1290
DpnII GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
DraIII CACNNNGTG 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 878, 1078
EarI CTCTTC 2 cut(s) 878, 1078
Eco130I CCWWGG 2 cut(s) 291, 1355
Eco147I AGGCCT 2 cut(s) 296, 765
Eco32I GATATC 1 cut(s) 1033
Eco47I GGWCC 1 cut(s) 1430
EcoRII CCWGG 2 cut(s) 54, 1425
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 2 cut(s) 291, 1355
EcoT22I ATGCAT 2 cut(s) 959, 1131
ErhI CCWWGG 2 cut(s) 291, 1355
FaeI CATG 9 cut(s) 197, 670, 723, 957, 1129, 1138, 1178, 1235, 1452
FatI CATG 9 cut(s) 193, 666, 719, 953, 1125, 1134, 1174, 1231, 1448
FauI CCCGC 1 cut(s) 998
FbaI TGATCA 1 cut(s) 939
Fnu4HI GCNGC 2 cut(s) 306, 745
FokI GGATG 6 cut(s) 440, 464, 717, 908, 931, 1132
Fsp4HI GCNGC 2 cut(s) 306, 745
FspBI CTAG 1 cut(s) 1271
GluI GCNGC 2 cut(s) 306, 745
GsaI CCCAGC 2 cut(s) 369, 1380
GsuI CTGGAG 2 cut(s) 534, 882
HaeIII GGCC 4 cut(s) 296, 466, 649, 765
HapII CCGG 4 cut(s) 181, 1208, 1299, 1433
Hin1II CATG 9 cut(s) 197, 670, 723, 957, 1129, 1138, 1178, 1235, 1452
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 8 cut(s) 322, 332, 522, 807, 818, 1052, 1065, 1213
HpaI GTTAAC 1 cut(s) 378
HpaII CCGG 4 cut(s) 181, 1208, 1299, 1433
HphI GGTGA 6 cut(s) 281, 388, 611, 988, 1222, 1298
Hpy166II GTNNAC 6 cut(s) 199, 378, 602, 642, 739, 1503
Hpy188I TCNGA 8 cut(s) 64, 321, 534, 676, 944, 989, 1064, 1218
Hpy188III TCNNGA 5 cut(s) 449, 685, 861, 1232, 1433
Hpy8I GTNNAC 6 cut(s) 199, 378, 602, 642, 739, 1503
HpyAV CCTTC 3 cut(s) 178, 1110, 1333
HpyCH4III ACNGT 4 cut(s) 203, 239, 494, 848
HpyCH4IV ACGT 1 cut(s) 946
HpyF10VI GCNNNNNNNGC 6 cut(s) 239, 248, 302, 980, 1411, 1495
HpyF3I CTNAG 8 cut(s) 111, 214, 320, 447, 673, 1026, 1245, 1401
HpySE526I ACGT 1 cut(s) 946
Hsp92II CATG 9 cut(s) 197, 670, 723, 957, 1129, 1138, 1178, 1235, 1452
Kpn2I TCCGGA 1 cut(s) 1432
KpnI GGTACC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 939
KspAI GTTAAC 1 cut(s) 378
Kzo9I GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
LmnI GCTCC 1 cut(s) 384
Lsp1109I GCAGC 2 cut(s) 317, 756
LweI GCATC 4 cut(s) 251, 325, 418, 909
MabI ACCWGGT 1 cut(s) 54
MaeI CTAG 1 cut(s) 1271
MaeII ACGT 1 cut(s) 946
MaeIII GTNAC 7 cut(s) 114, 565, 784, 848, 1252, 1318, 1451
MalI GATC 8 cut(s) 346, 477, 659, 941, 1010, 1171, 1194, 1290
MboI GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
MboII GAAGA 5 cut(s) 531, 895, 1095, 1450, 1475
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 2 cut(s) 344, 1008
MhlI GDGCHC 1 cut(s) 1505
MluCI AATT 6 cut(s) 309, 585, 617, 709, 837, 1225
MlyI GAGTC 2 cut(s) 331, 812
MmeI TCCRAC 1 cut(s) 717
Mph1103I ATGCAT 2 cut(s) 959, 1131
MroI TCCGGA 1 cut(s) 1432
MseI TTAA 4 cut(s) 14, 377, 635, 1280
MslI CAYNNNNRTG 2 cut(s) 426, 878
MspA1I CMGCKG 2 cut(s) 512, 1498
MspI CCGG 4 cut(s) 181, 1208, 1299, 1433
MspR9I CCNGG 3 cut(s) 56, 1300, 1427
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 2 cut(s) 56, 1427
MwoI GCNNNNNNNGC 6 cut(s) 239, 248, 302, 980, 1411, 1495
NciI CCSGG 1 cut(s) 1300
NdeII GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
NlaIII CATG 9 cut(s) 197, 670, 723, 957, 1129, 1138, 1178, 1235, 1452
NlaIV GGNNCC 6 cut(s) 277, 346, 544, 1010, 1398, 1431
NmeAIII GCCGAG 1 cut(s) 625
NmuCI GTSAC 1 cut(s) 784
NsiI ATGCAT 2 cut(s) 959, 1131
NspI RCATGY 4 cut(s) 197, 957, 1178, 1452
PaeI GCATGC 1 cut(s) 1178
PagI TCATGA 1 cut(s) 1231
PceI AGGCCT 2 cut(s) 296, 765
PciI ACATGT 1 cut(s) 193
PfeI GAWTC 6 cut(s) 332, 522, 807, 1052, 1065, 1213
Pfl23II CGTACG 1 cut(s) 489
PkrI GCNGC 2 cut(s) 307, 746
PleI GAGTC 2 cut(s) 330, 812
PpsI GAGTC 2 cut(s) 330, 812
PscI ACATGT 1 cut(s) 193
PshAI GACNNNNGTC 1 cut(s) 1428
Psp6I CCWGG 2 cut(s) 54, 1425
PspFI CCCAGC 2 cut(s) 365, 1376
PspGI CCWGG 2 cut(s) 54, 1425
PspLI CGTACG 1 cut(s) 489
PspN4I GGNNCC 6 cut(s) 277, 346, 544, 1010, 1398, 1431
PspPI GGNCC 1 cut(s) 1430
PsrI GAACNNNNNNTAC 2 cut(s) 129, 161
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 2 cut(s) 344, 1008
PvuII CAGCTG 2 cut(s) 512, 1498
RsaI GTAC 7 cut(s) 138, 212, 277, 435, 491, 641, 1353
RsaNI GTAC 7 cut(s) 137, 211, 276, 434, 490, 640, 1352
RseI CAYNNNNRTG 2 cut(s) 426, 878
SaqAI TTAA 4 cut(s) 14, 377, 635, 1280
SatI GCNGC 2 cut(s) 306, 745
Sau3AI GATC 8 cut(s) 344, 475, 657, 939, 1008, 1169, 1192, 1288
Sau96I GGNCC 1 cut(s) 1430
ScaI AGTACT 2 cut(s) 138, 212
SchI GAGTC 2 cut(s) 331, 812
ScrFI CCNGG 3 cut(s) 56, 1300, 1427
SduI GDGCHC 1 cut(s) 1505
SexAI ACCWGGT 1 cut(s) 54
SfaNI GCATC 4 cut(s) 251, 325, 418, 909
SfcI CTRYAG 1 cut(s) 1077
SinI GGWCC 1 cut(s) 1430
SmiMI CAYNNNNRTG 2 cut(s) 426, 878
SphI GCATGC 1 cut(s) 1178
Sse9I AATT 6 cut(s) 309, 585, 617, 709, 837, 1225
SseBI AGGCCT 2 cut(s) 296, 765
SsiI CCGC 3 cut(s) 622, 974, 1005
SspMI CTAG 1 cut(s) 1271
StuI AGGCCT 2 cut(s) 296, 765
StyD4I CCNGG 3 cut(s) 54, 1298, 1425
StyI CCWWGG 2 cut(s) 291, 1355
TaaI ACNGT 4 cut(s) 203, 239, 494, 848
TaiI ACGT 1 cut(s) 949
TaqI TCGA 3 cut(s) 496, 660, 835
TasI AATT 6 cut(s) 309, 585, 617, 709, 837, 1225
TatI WGTACW 4 cut(s) 136, 210, 433, 639
TfiI GAWTC 6 cut(s) 332, 522, 807, 1052, 1065, 1213
Tru1I TTAA 4 cut(s) 14, 377, 635, 1280
Tru9I TTAA 4 cut(s) 14, 377, 635, 1280
TscAI CASTG 4 cut(s) 252, 559, 789, 1372
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 2 cut(s) 305, 744
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 6 cut(s) 17, 345, 474, 514, 694, 1151
TspGWI ACGGA 2 cut(s) 183, 1064
TspRI CASTG 4 cut(s) 252, 559, 789, 1372
VneI GTGCAC 1 cut(s) 1501
VpaK11BI GGWCC 1 cut(s) 1430
XapI RAATTY 1 cut(s) 617
XceI RCATGY 4 cut(s) 197, 957, 1178, 1452
XspI CTAG 1 cut(s) 1271
ZrmI AGTACT 2 cut(s) 138, 212
Zsp2I ATGCAT 2 cut(s) 959, 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.