RchiOBHm_Chr7g0196791

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008948
Physical Location & Seq
Reverse (-)
14756782 .. 14759928
3147 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17604

Sequence Viewer

Length: 1446 bp
ATGGGACTTTGGAGGACTCCCCCAAAAGCTCAGTTACCTAAGATGGCTATGGTGGTACTACTGGTTCTGGTTTCATTGTGCACAAACGGAGTTGAAAGCCGGAAGGCTAAGAATGTGGACAGCTTTGAGTACTCAGCCATAAGTTGCAGAGCCCAAAGTGCATCACTGGCAGATTTTGGAGGAGTTGGTGATGGTATCACATCAAACACCAAGGCTTTTCAGGCAGCAATTCAGCATCTGAGTCAATATGACTCACAAGGTGGATCCCAACTTTTTGTTCCTGCTGGGAAATGGTTAACTGGGAGCTTCAACCTCACGAGCCATTTCACTCTCTATCTCCACAAGGATGCTGTTCTTCTTGCCTCTCAGGATGAGAATGAATGGCCAGTGATTGATCCACTTCCATCGTACGGTAGAGGAAGGGATACTGCTGGAGGAAGATTCATCAGTCTGATATTTGGAACCAACCTCACTGATGTTGTAGTAACAGGGGAAAATGGCACAATTGATGGTCAAGGTGAACTATGGTGGCAAAAATTCCACAAGGGGGAGTTAAAGTACACTCGGCCTTACCTGATTGAAATCATGTTCTCAGAAAATATCCAAATTTCGAACCTTACTTTGGTTAATTCTCCATCATGGAATGTTCATCCTGTTTACAGCAGCAATGTTCTAGTTCAAGGCCTTACAATCCTTGCACCAGTAACGTCTCCAAACACAGATGGGATCAACCCCGACTCTTGCACAAATACTCGAATTGAGGACTGTTACATTGTCTCTGGAGATGATTGTATAGCCGTAAAGAGTGGTTGGGATGAGTATGGTATTGCCTTTGGGATGCCAACCAAAGCGCTTGTGATCAGACGTTTGACATGCATTTCTCCATTTAGTGCTGTGATTGCACTCGGAAGTGAGATGTCTGGTGGGATCCAAGATGTCAGGGCTGAGGATATCCTTGCCATTAACACGGAATCAGGAGTCAGAATCAAAACTGCTGTAGGAAGAGGAGGCTTTGTGAAAGACATATATGTCAGAGGGATGACCATGAAAACCATGAAGTATGTATTTTGGATTGCAGGAGACTATGGATCACATGCAGATGATGGCTATGATCCTAATGCGCTTCCAGTGATCCAGAACATAAATTATCATGATATGGTGGCTGAGAATGTAACAATGGCAGGTAAACTGGGGGGAATAACTGGAGATCCATTTACTGGGATTTGCATATCTAATGTTACAATTACAATGGCAAAGAAGGGAAAGAAAGTACCTTGGAACTGTACTGACGTTGCTGGGATTTCAAGCAGAGTGGTTCCTCAGCCGTGTGAGCTTCTAGCAGACCAGGGTCCGGAGAAGATCGGGGCATGTGATTTCCCAGAAGAAAGTCTTCCGGTTGATAATATGCAACTTCAGCTCTGCTCTTACAGAGGAAAGTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

481

Amino Acids

52.26

Weight (kDa)

5.37

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 151 - 431 6.5e-39 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1028
Acc36I ACCTGC 1 cut(s) 1172
AccB7I CCANNNNNTGG 1 cut(s) 1217
AccIII TCCGGA 1 cut(s) 1351
AcoI YGGCCR 1 cut(s) 383
AcsI RAATTY 2 cut(s) 536, 606
AcuI CTGAAG 1 cut(s) 1397
AdeI CACNNNGTG 1 cut(s) 260
AfaI GTAC 7 cut(s) 57, 131, 410, 560, 1272, 1285, 1439
AfeI AGCGCT 1 cut(s) 852
AfiI CCNNNNNNNGG 5 cut(s) 410, 547, 622, 1217, 1351
AgsI TTSAA 5 cut(s) 95, 310, 581, 680, 1305
AjnI CCWGG 1 cut(s) 1344
AjuI GAANNNNNNNTTGG 4 cut(s) 261, 293, 605, 637
AluBI AGCT 5 cut(s) 29, 123, 306, 1333, 1417
AluI AGCT 5 cut(s) 29, 123, 306, 1333, 1417
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 3 cut(s) 714, 781, 1074
Alw44I GTGCAC 1 cut(s) 79
AlwNI CAGNNNCTG 1 cut(s) 238
Aor13HI TCCGGA 1 cut(s) 1351
Aor51HI AGCGCT 1 cut(s) 852
AoxI GGCC 3 cut(s) 383, 566, 682
ApaLI GTGCAC 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 224, 663
ApoI RAATTY 2 cut(s) 536, 606
Asp700I GAANNNNTTC 1 cut(s) 1389
AspLEI GCGC 2 cut(s) 853, 1123
AspS9I GGNCC 1 cut(s) 1349
AsuHPI GGTGA 2 cut(s) 200, 530
AsuII TTCGAA 1 cut(s) 611
AvaII GGWCC 1 cut(s) 1349
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 1 cut(s) 385
BamHI GGATCC 2 cut(s) 263, 927
BanII GRGCYC 1 cut(s) 154
BauI CACGAG 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 1382
Bbv12I GWGCWC 1 cut(s) 83
BbvCI CCTCAGC 2 cut(s) 945, 1320
BbvI GCAGC 2 cut(s) 236, 675
BccI CCATC 7 cut(s) 37, 185, 412, 503, 643, 716, 1097
BceAI ACGGC 2 cut(s) 782, 1309
BciT130I CCWGG 1 cut(s) 1346
BciVI GTATCC 1 cut(s) 418
BclI TGATCA 1 cut(s) 858
BcoDI GTCTC 3 cut(s) 714, 781, 1074
BfaI CTAG 2 cut(s) 674, 1337
BfmI CTRYAG 1 cut(s) 996
BfoI RGCGCY 1 cut(s) 854
BfuAI ACCTGC 1 cut(s) 1172
BfuI GTATCC 1 cut(s) 418
BisI GCNGC 2 cut(s) 225, 664
BlsI GCNGC 2 cut(s) 226, 665
BmcAI AGTACT 2 cut(s) 131, 1439
Bme1390I CCNGG 1 cut(s) 1346
Bme18I GGWCC 1 cut(s) 1349
BmgT120I GGNCC 1 cut(s) 1349
BmiI GGNNCC 5 cut(s) 265, 463, 929, 1317, 1350
BmrFI CCNGG 1 cut(s) 1346
BmrI ACTGGG 3 cut(s) 309, 1199, 1227
BmsI GCATC 4 cut(s) 170, 244, 337, 828
BmuI ACTGGG 3 cut(s) 309, 1199, 1227
BoxI GACNNNNGTC 1 cut(s) 1347
BpiI GAAGAC 1 cut(s) 1382
BpmI CTGGAG 3 cut(s) 453, 801, 1224
Bpu10I CCTNAGC 2 cut(s) 945, 1320
Bpu14I TTCGAA 1 cut(s) 611
BsaBI GATNNNNATC 1 cut(s) 581
BsaJI CCNNGG 3 cut(s) 210, 1274, 1345
BsaWI WCCGGW 2 cut(s) 1351, 1393
BsaXI ACNNNNNCTCC 4 cut(s) 542, 572, 999, 1029
Bsc4I CCNNNNNNNGG 5 cut(s) 410, 547, 622, 1217, 1351
Bse3DI GCAATG 1 cut(s) 673
Bse8I GATNNNNATC 1 cut(s) 581
BseAI TCCGGA 1 cut(s) 1351
BseBI CCWGG 1 cut(s) 1346
BseDI CCNNGG 3 cut(s) 210, 1274, 1345
BseGI GGATG 6 cut(s) 352, 376, 649, 820, 843, 1044
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 5 cut(s) 410, 547, 622, 1217, 1351
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 8 cut(s) 44, 147, 230, 380, 606, 936, 1155, 1334
BseRI GAGGAG 2 cut(s) 195, 1020
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 2 cut(s) 236, 675
BseYI CCCAGC 2 cut(s) 284, 1295
BshFI GGCC 3 cut(s) 385, 568, 684
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 3 cut(s) 100, 1352, 1394
BsiWI CGTACG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 5 cut(s) 410, 547, 622, 1217, 1351
BsmAI GTCTC 3 cut(s) 714, 781, 1074
BsmBI CGTCTC 1 cut(s) 714
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 3 cut(s) 385, 568, 684
Bsp119I TTCGAA 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 83, 154
Bsp13I TCCGGA 1 cut(s) 1351
BspANI GGCC 3 cut(s) 385, 568, 684
BspCNI CTCAG 8 cut(s) 43, 146, 231, 379, 605, 937, 1156, 1333
BspEI TCCGGA 1 cut(s) 1351
BspHI TCATGA 1 cut(s) 1150
BspLI GGNNCC 5 cut(s) 265, 463, 929, 1317, 1350
BspMI ACCTGC 1 cut(s) 1172
BspT104I TTCGAA 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 673
BssECI CCNNGG 3 cut(s) 210, 1274, 1345
BssSI CACGAG 1 cut(s) 316
BssT1I CCWWGG 2 cut(s) 210, 1274
Bst2BI CACGAG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 1346
Bst4CI ACNGT 3 cut(s) 413, 767, 1283
Bst6I CTCTTC 1 cut(s) 997
BstBI TTCGAA 1 cut(s) 611
BstF5I GGATG 6 cut(s) 352, 376, 649, 820, 843, 1044
BstH2I RGCGCY 1 cut(s) 854
BstHHI GCGC 2 cut(s) 853, 1123
BstMAI GTCTC 3 cut(s) 714, 781, 1074
BstMWI GCNNNNNNNGC 6 cut(s) 158, 167, 221, 899, 1330, 1414
BstNI CCWGG 1 cut(s) 1346
BstNSI RCATGY 3 cut(s) 876, 1097, 1371
BstPAI GACNNNNGTC 1 cut(s) 1347
BstSCI CCNGG 1 cut(s) 1344
BstSFI CTRYAG 1 cut(s) 996
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 236, 675
BstV2I GAAGAC 1 cut(s) 1382
BstX2I RGATCY 3 cut(s) 263, 927, 1207
BstYI RGATCY 3 cut(s) 263, 927, 1207
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 3 cut(s) 385, 568, 684
BtsCI GGATG 6 cut(s) 352, 376, 649, 820, 843, 1044
BtsIMutI CAGTG 4 cut(s) 164, 393, 471, 1134
BveI ACCTGC 1 cut(s) 1172
CaiI CAGNNNCTG 1 cut(s) 238
CciI TCATGA 1 cut(s) 1150
CfoI GCGC 2 cut(s) 853, 1123
Cfr13I GGNCC 1 cut(s) 1349
Csp6I GTAC 7 cut(s) 56, 130, 409, 559, 1271, 1284, 1438
CspCI CAANNNNNGTGG 2 cut(s) 1293, 1328
CviAII CATG 8 cut(s) 586, 639, 873, 1045, 1054, 1094, 1151, 1368
CviQI GTAC 7 cut(s) 56, 130, 409, 559, 1271, 1284, 1438
DraIII CACNNNGTG 1 cut(s) 260
DrdI GACNNNNNNGTC 1 cut(s) 1028
DseDI GACNNNNNNGTC 1 cut(s) 1028
EaeI YGGCCR 1 cut(s) 383
Eam1104I CTCTTC 1 cut(s) 997
EarI CTCTTC 1 cut(s) 997
Eco130I CCWWGG 2 cut(s) 210, 1274
Eco147I AGGCCT 1 cut(s) 684
Eco24I GRGCYC 1 cut(s) 154
Eco32I GATATC 1 cut(s) 952
Eco47I GGWCC 1 cut(s) 1349
Eco47III AGCGCT 1 cut(s) 852
Eco57I CTGAAG 1 cut(s) 1397
EcoRII CCWGG 1 cut(s) 1344
EcoRV GATATC 1 cut(s) 952
EcoT14I CCWWGG 2 cut(s) 210, 1274
EcoT22I ATGCAT 1 cut(s) 878
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 2 cut(s) 210, 1274
Esp3I CGTCTC 1 cut(s) 714
FaeI CATG 8 cut(s) 589, 642, 876, 1048, 1057, 1097, 1154, 1371
FalI AAGNNNNNCTT 2 cut(s) 1374, 1406
FaqI GGGAC 1 cut(s) 18
FatI CATG 8 cut(s) 585, 638, 872, 1044, 1053, 1093, 1150, 1367
FbaI TGATCA 1 cut(s) 858
Fnu4HI GCNGC 2 cut(s) 225, 664
FokI GGATG 6 cut(s) 359, 383, 636, 827, 850, 1051
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 2 cut(s) 225, 664
FspBI CTAG 2 cut(s) 674, 1337
GlaI GCGC 2 cut(s) 852, 1122
GluI GCNGC 2 cut(s) 225, 664
GsaI CCCAGC 2 cut(s) 288, 1299
GsuI CTGGAG 3 cut(s) 453, 801, 1224
HaeII RGCGCY 1 cut(s) 854
HaeIII GGCC 3 cut(s) 385, 568, 684
HapII CCGG 3 cut(s) 100, 1352, 1394
HhaI GCGC 2 cut(s) 853, 1123
Hin1II CATG 8 cut(s) 589, 642, 876, 1048, 1057, 1097, 1154, 1371
Hin6I GCGC 2 cut(s) 851, 1121
HinP1I GCGC 2 cut(s) 851, 1121
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HinfI GANTC 8 cut(s) 16, 241, 251, 441, 737, 971, 978, 984
HpaI GTTAAC 1 cut(s) 297
HpaII CCGG 3 cut(s) 100, 1352, 1394
HphI GGTGA 2 cut(s) 200, 530
Hpy166II GTNNAC 7 cut(s) 81, 118, 297, 521, 561, 658, 1187
Hpy188I TCNGA 7 cut(s) 240, 453, 595, 863, 908, 983, 1034
Hpy188III TCNNGA 7 cut(s) 316, 368, 780, 975, 1135, 1151, 1352
Hpy8I GTNNAC 7 cut(s) 81, 118, 297, 521, 561, 658, 1187
HpyAV CCTTC 3 cut(s) 97, 414, 1252
HpyCH4III ACNGT 3 cut(s) 413, 767, 1283
HpyCH4IV ACGT 3 cut(s) 707, 865, 1290
HpyF10VI GCNNNNNNNGC 6 cut(s) 158, 167, 221, 899, 1330, 1414
HpySE526I ACGT 3 cut(s) 707, 865, 1290
Hsp92II CATG 8 cut(s) 589, 642, 876, 1048, 1057, 1097, 1154, 1371
HspAI GCGC 2 cut(s) 851, 1121
Kpn2I TCCGGA 1 cut(s) 1351
Ksp22I TGATCA 1 cut(s) 858
KspAI GTTAAC 1 cut(s) 297
LmnI GCTCC 1 cut(s) 303
Lsp1109I GCAGC 2 cut(s) 236, 675
LweI GCATC 4 cut(s) 170, 244, 337, 828
MaeI CTAG 2 cut(s) 674, 1337
MaeII ACGT 3 cut(s) 707, 865, 1290
MaeIII GTNAC 6 cut(s) 33, 484, 703, 767, 1171, 1237
MboII GAAGA 6 cut(s) 347, 450, 1014, 1369, 1382, 1394
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 3 cut(s) 263, 927, 1207
MhlI GDGCHC 2 cut(s) 83, 154
MlsI TGGCCA 1 cut(s) 385
MluCI AATT 8 cut(s) 228, 504, 536, 606, 628, 756, 1144, 1242
MluNI TGGCCA 1 cut(s) 385
MlyI GAGTC 5 cut(s) 10, 245, 250, 731, 987
Mox20I TGGCCA 1 cut(s) 385
Mph1103I ATGCAT 1 cut(s) 878
MroI TCCGGA 1 cut(s) 1351
MroXI GAANNNNTTC 1 cut(s) 1389
MscI TGGCCA 1 cut(s) 385
MseI TTAA 4 cut(s) 296, 554, 627, 963
MslI CAYNNNNRTG 2 cut(s) 345, 1098
Msp20I TGGCCA 1 cut(s) 385
MspI CCGG 3 cut(s) 100, 1352, 1394
MspR9I CCNGG 1 cut(s) 1346
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 1 cut(s) 1346
MwoI GCNNNNNNNGC 6 cut(s) 158, 167, 221, 899, 1330, 1414
NlaIII CATG 8 cut(s) 589, 642, 876, 1048, 1057, 1097, 1154, 1371
NlaIV GGNNCC 5 cut(s) 265, 463, 929, 1317, 1350
NmeAIII GCCGAG 1 cut(s) 544
NsiI ATGCAT 1 cut(s) 878
NspI RCATGY 3 cut(s) 876, 1097, 1371
NspV TTCGAA 1 cut(s) 611
PagI TCATGA 1 cut(s) 1150
PceI AGGCCT 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 1389
PfeI GAWTC 3 cut(s) 441, 971, 984
Pfl23II CGTACG 1 cut(s) 408
PflMI CCANNNNNTGG 1 cut(s) 1217
PkrI GCNGC 2 cut(s) 226, 665
PleI GAGTC 5 cut(s) 10, 245, 249, 731, 986
PpsI GAGTC 5 cut(s) 10, 245, 249, 731, 986
PshAI GACNNNNGTC 1 cut(s) 1347
Psp6I CCWGG 1 cut(s) 1344
PspFI CCCAGC 2 cut(s) 284, 1295
PspGI CCWGG 1 cut(s) 1344
PspLI CGTACG 1 cut(s) 408
PspN4I GGNNCC 5 cut(s) 265, 463, 929, 1317, 1350
PspPI GGNCC 1 cut(s) 1349
PsrI GAACNNNNNNTAC 2 cut(s) 48, 80
PstNI CAGNNNCTG 1 cut(s) 238
PsuI RGATCY 3 cut(s) 263, 927, 1207
RsaI GTAC 7 cut(s) 57, 131, 410, 560, 1272, 1285, 1439
RsaNI GTAC 7 cut(s) 56, 130, 409, 559, 1271, 1284, 1438
RseI CAYNNNNRTG 2 cut(s) 345, 1098
SaqAI TTAA 4 cut(s) 296, 554, 627, 963
SatI GCNGC 2 cut(s) 225, 664
Sau96I GGNCC 1 cut(s) 1349
ScaI AGTACT 2 cut(s) 131, 1439
SchI GAGTC 5 cut(s) 10, 245, 250, 731, 987
ScrFI CCNGG 1 cut(s) 1346
SduI GDGCHC 2 cut(s) 83, 154
SfaNI GCATC 4 cut(s) 170, 244, 337, 828
SfcI CTRYAG 1 cut(s) 996
SfuI TTCGAA 1 cut(s) 611
SinI GGWCC 1 cut(s) 1349
SmiMI CAYNNNNRTG 2 cut(s) 345, 1098
Sse9I AATT 8 cut(s) 228, 504, 536, 606, 628, 756, 1144, 1242
SseBI AGGCCT 1 cut(s) 684
SspMI CTAG 2 cut(s) 674, 1337
StuI AGGCCT 1 cut(s) 684
StyD4I CCNGG 1 cut(s) 1344
StyI CCWWGG 2 cut(s) 210, 1274
TaaI ACNGT 3 cut(s) 413, 767, 1283
TaiI ACGT 3 cut(s) 710, 868, 1293
TaqI TCGA 2 cut(s) 611, 754
TasI AATT 8 cut(s) 228, 504, 536, 606, 628, 756, 1144, 1242
TatI WGTACW 4 cut(s) 129, 558, 1283, 1437
TfiI GAWTC 3 cut(s) 441, 971, 984
Tru1I TTAA 4 cut(s) 296, 554, 627, 963
Tru9I TTAA 4 cut(s) 296, 554, 627, 963
TscAI CASTG 4 cut(s) 171, 393, 478, 1134
TseI GCWGC 2 cut(s) 224, 663
TspDTI ATGAA 6 cut(s) 63, 393, 433, 638, 1061, 1070
TspGWI ACGGA 2 cut(s) 102, 983
TspRI CASTG 4 cut(s) 171, 393, 478, 1134
Van91I CCANNNNNTGG 1 cut(s) 1217
VneI GTGCAC 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 1349
XapI RAATTY 2 cut(s) 536, 606
XceI RCATGY 3 cut(s) 876, 1097, 1371
XmnI GAANNNNTTC 1 cut(s) 1389
XspI CTAG 2 cut(s) 674, 1337
ZrmI AGTACT 2 cut(s) 131, 1439
Zsp2I ATGCAT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.