MD14G1110300.v1.1

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008948
Physical Location & Seq
Forward (+)
17532064 .. 17535772
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1110300.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGAGGAGGACTCCCATGAATACTCAGTCATCTGAGATGGTGATGGCAGTAGTATTTGTTGTTTTATTGGCCACAACAAAAGGAGTTGAAAGCCGCAAGGCTCGGAGGGAGAATGCAGGAGGAGAATACTTCGAGTACTGCGCCATAAGTTGCAGAGCCAAAAGTGCTTCTCTGACAGATTATGGAGGAGTTGGCGATGGAACGACATCAAACACTAAGGCTTTTCAAGAAGCAATCACTCATCTGAGTCAGTACAAGTCAGAAGGCGGGTCACAACTCGTTGTCCCTCCTGGTAAATGGTTAACTGGCAGCTTCAACCTCACCAGCCATTTCACGCTTTATCTCCATAAGGATGCAGTTCTTCTTGCTTCTCAGGACGAGAGCGAATGGCCGGTGATTGAACCCCTTCCGTCGTACGGCCGAGGAAGGGACAACACAGATGGTGGAAGATTCATCAGTCTGATATTTGGAACCAATCTCACTGACGTTGTAATAACAGGTGACAATGGCACCATCGATGGCCAAGGTGAAGTATGGTGGCAGAAATTCAAAAAGGGACAGTTAAATTACACGCGACCGTACCTGATCGAAATCATGTACTCCGAAAACATCCAGATTTCCAACCTCACTTTGGTTAATTCTCCTTCATGGAATGTTCATCCCATTTACAGCAGCAATGTTCTTGTTCAAGGCATTACAATTCTTGCACCAGTGACATCTCCAAACACAGATGGGATCAACCCCGATTCTTGCACAAATACTCGAATTGAGGACTGCTACATTGTCTCTGGGGATGATTGCATAGCAGTAAAGAGCGGTTGGGATGAGTATGGAATTGCTTTCGGGATGCCTACGAAACAGCTCGTGATCAGACGGCTGACATGCATTTCTCCATTCAGTGCAGTGATTGCACTTGGGAGCGAGATGTCAGGTGGAATCCAAGATGTGAGGGCGGAGGATATTTTTGCCATTGACTCGGAATCAGGAGTTCGAATCAAAACTGCTGTGGGAAGAGGAGGGTTTGTGAAAGACATATACGTGAGAGGGATGACTATGAAAACAATGAAATGGGCGTTTTGGATGACAGGAAACTACGGATCCCATGCAGATAATGGCTACGATCCGAATGCCCTTCCTGCGATTCAGAATATAAATTATCATGACATGGTTGCTGAGAATGTGACAATGGCAGCTAAGTTGGAGGGGATTCCTGGCGATCCGTTTACGGGGATATGCATATCGAACGTTACGATTACACTGGCAAAGAAGGCGAAGAAATTACCGTGGAATTGCACTGATATTGCTGGGATTTCAAGCGGCGTGGTTCCTCGGCCATGTGAGGCTCTGGCAGACCAGGGTCCAGGCAATGTTACAGCGTGCAATTTTCCAGAAGAGGGCCTGCCAATTGATAATGTTCAAGTTCAGGTGTGCTCTTACAGGAGGGAGCACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

485

Amino Acids

52.81

Weight (kDa)

5.41

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 153 - 426 8e-38 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AccBSI CCGCTC 1 cut(s) 816
AccII CGCG 1 cut(s) 574
AciI CCGC 5 cut(s) 94, 267, 816, 953, 1317
AclI AACGTT 1 cut(s) 1245
AclWI GGATC 5 cut(s) 743, 1092, 1105, 1115, 1211
AcoI YGGCCR 5 cut(s) 69, 389, 418, 520, 1331
AcsI RAATTY 1 cut(s) 545
AfaI GTAC 5 cut(s) 137, 254, 416, 581, 599
AfiI CCNNNNNNNGG 5 cut(s) 416, 427, 631, 1226, 1394
AgsI TTSAA 8 cut(s) 89, 227, 316, 401, 550, 689, 1314, 1418
AjnI CCWGG 4 cut(s) 289, 1210, 1353, 1360
AluBI AGCT 3 cut(s) 312, 862, 1193
AluI AGCT 3 cut(s) 312, 862, 1193
Alw21I GWGCWC 2 cut(s) 1433, 1449
Alw26I GTCTC 1 cut(s) 790
AlwI GGATC 5 cut(s) 743, 1092, 1105, 1115, 1211
AoxI GGCC 6 cut(s) 69, 389, 418, 520, 1331, 1396
ApeKI GCWGC 3 cut(s) 309, 672, 1190
ApoI RAATTY 1 cut(s) 545
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 2 cut(s) 1358, 1396
AsuHPI GGTGA 5 cut(s) 52, 313, 406, 512, 539
AsuII TTCGAA 1 cut(s) 991
AvaII GGWCC 1 cut(s) 1358
BalI TGGCCA 2 cut(s) 71, 522
BamHI GGATCC 1 cut(s) 1097
BanI GGYRCC 1 cut(s) 509
BauI CACGAG 1 cut(s) 863
Bbv12I GWGCWC 2 cut(s) 1433, 1449
BbvI GCAGC 3 cut(s) 321, 684, 1202
BccI CCATC 7 cut(s) 31, 37, 191, 434, 512, 521, 725
BceAI ACGGC 2 cut(s) 433, 890
BcgI CGANNNNNNTGC 2 cut(s) 1109, 1143
BciT130I CCWGG 4 cut(s) 291, 1212, 1355, 1362
BclI TGATCA 1 cut(s) 867
BcoDI GTCTC 1 cut(s) 790
BisI GCNGC 5 cut(s) 94, 310, 673, 1191, 1318
BlsI GCNGC 5 cut(s) 95, 311, 674, 1192, 1319
BmcAI AGTACT 1 cut(s) 137
Bme1390I CCNGG 4 cut(s) 291, 1212, 1355, 1362
Bme18I GGWCC 1 cut(s) 1358
BmgT120I GGNCC 2 cut(s) 1358, 1396
BmiI GGNNCC 5 cut(s) 472, 511, 1099, 1326, 1359
BmrFI CCNGG 4 cut(s) 291, 1212, 1355, 1362
BmsI GCATC 2 cut(s) 343, 837
BoxI GACNNNNGTC 1 cut(s) 1356
BplI GAGNNNNNCTC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 991
Bsa29I ATCGAT 1 cut(s) 516
BsaAI YACGTR 1 cut(s) 1039
BsaBI GATNNNNATC 1 cut(s) 590
BsaJI CCNNGG 5 cut(s) 421, 523, 1283, 1328, 1354
BsaXI ACNNNNNCTCC 3 cut(s) 1008, 1038, 1436
Bsc4I CCNNNNNNNGG 5 cut(s) 416, 427, 631, 1226, 1394
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 4 cut(s) 310, 710, 1263, 1454
Bse3DI GCAATG 2 cut(s) 682, 1372
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 4 cut(s) 291, 1212, 1355, 1362
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 5 cut(s) 421, 523, 1283, 1328, 1354
BseGI GGATG 8 cut(s) 358, 609, 658, 799, 829, 852, 1053, 1086
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 5 cut(s) 416, 427, 631, 1226, 1394
BseMI GCAATG 2 cut(s) 682, 1372
BseMII CTCAG 5 cut(s) 24, 38, 236, 386, 1164
BseNI ACTGG 4 cut(s) 310, 710, 1263, 1454
BseRI GAGGAG 4 cut(s) 19, 135, 201, 1029
BseX3I CGGCCG 1 cut(s) 418
BseXI GCAGC 3 cut(s) 321, 684, 1202
BseYI CCCAGC 1 cut(s) 1304
BsgI GTGCAG 1 cut(s) 921
Bsh1236I CGCG 1 cut(s) 574
Bsh1285I CGRYCG 2 cut(s) 421, 578
BshFI GGCC 6 cut(s) 71, 391, 420, 522, 1333, 1398
BshNI GGYRCC 1 cut(s) 509
BshVI ATCGAT 1 cut(s) 516
BsiEI CGRYCG 2 cut(s) 421, 578
BsiHKAI GWGCWC 2 cut(s) 1433, 1449
BsiSI CCGG 1 cut(s) 392
BsiWI CGTACG 1 cut(s) 414
BslFI GGGAC 3 cut(s) 269, 443, 570
BslI CCNNNNNNNGG 5 cut(s) 416, 427, 631, 1226, 1394
BsmAI GTCTC 1 cut(s) 790
BsmFI GGGAC 3 cut(s) 269, 443, 570
BsmI GAATGC 2 cut(s) 118, 1132
BsnI GGCC 6 cut(s) 71, 391, 420, 522, 1333, 1398
Bsp119I TTCGAA 1 cut(s) 991
Bsp1286I GDGCHC 2 cut(s) 1433, 1449
Bsp143I GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
BspACI CCGC 5 cut(s) 94, 267, 816, 953, 1317
BspANI GGCC 6 cut(s) 71, 391, 420, 522, 1333, 1398
BspCNI CTCAG 5 cut(s) 25, 37, 237, 385, 1165
BspDI ATCGAT 1 cut(s) 516
BspFNI CGCG 1 cut(s) 574
BspHI TCATGA 1 cut(s) 1159
BspLI GGNNCC 5 cut(s) 472, 511, 1099, 1326, 1359
BspPI GGATC 5 cut(s) 743, 1092, 1105, 1115, 1211
BspT104I TTCGAA 1 cut(s) 991
BspT107I GGYRCC 1 cut(s) 509
BsrBI CCGCTC 1 cut(s) 816
BsrDI GCAATG 2 cut(s) 682, 1372
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 4 cut(s) 310, 710, 1263, 1454
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 5 cut(s) 421, 523, 1283, 1328, 1354
BssMI GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
BssSI CACGAG 1 cut(s) 863
BssT1I CCWWGG 1 cut(s) 523
Bst2BI CACGAG 1 cut(s) 863
Bst2UI CCWGG 4 cut(s) 291, 1212, 1355, 1362
Bst4CI ACNGT 3 cut(s) 561, 579, 1284
Bst6I CTCTTC 2 cut(s) 1006, 1386
BstAPI GCANNNNNTGC 1 cut(s) 908
BstBAI YACGTR 1 cut(s) 1039
BstBI TTCGAA 1 cut(s) 991
BstC8I GCNNGC 2 cut(s) 1378, 1400
BstDEI CTNAG 7 cut(s) 24, 33, 216, 245, 372, 1173, 1194
BstDSI CCRYGG 1 cut(s) 1283
BstF5I GGATG 8 cut(s) 358, 609, 658, 799, 829, 852, 1053, 1086
BstFNI CGCG 1 cut(s) 574
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 6 cut(s) 588, 738, 870, 1100, 1123, 1219
BstMAI GTCTC 1 cut(s) 790
BstMBI GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
BstMCI CGRYCG 2 cut(s) 421, 578
BstMWI GCNNNNNNNGC 4 cut(s) 164, 908, 1136, 1268
BstNI CCWGG 4 cut(s) 291, 1212, 1355, 1362
BstNSI RCATGY 1 cut(s) 885
BstPAI GACNNNNGTC 1 cut(s) 1356
BstSCI CCNGG 4 cut(s) 289, 1210, 1353, 1360
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 3 cut(s) 321, 684, 1202
BstX2I RGATCY 1 cut(s) 1097
BstYI RGATCY 1 cut(s) 1097
BstZI CGGCCG 1 cut(s) 418
Bsu15I ATCGAT 1 cut(s) 516
BsuRI GGCC 6 cut(s) 71, 391, 420, 522, 1333, 1398
BsuTUI ATCGAT 1 cut(s) 516
BtgI CCRYGG 1 cut(s) 1283
BtgZI GCGATG 1 cut(s) 210
BtsCI GGATG 8 cut(s) 358, 609, 658, 799, 829, 852, 1053, 1086
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 7 cut(s) 480, 717, 904, 909, 1256, 1293, 1447
Cac8I GCNNGC 2 cut(s) 1378, 1400
CciI TCATGA 1 cut(s) 1159
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 1358, 1396
ClaI ATCGAT 1 cut(s) 516
Csp6I GTAC 5 cut(s) 136, 253, 415, 580, 598
CspCI CAANNNNNGTGG 2 cut(s) 1302, 1337
CviAII CATG 8 cut(s) 16, 595, 648, 882, 1103, 1160, 1165, 1335
CviQI GTAC 5 cut(s) 136, 253, 415, 580, 598
DdeI CTNAG 7 cut(s) 24, 33, 216, 245, 372, 1173, 1194
DpnI GATC 6 cut(s) 587, 737, 869, 1099, 1122, 1218
DpnII GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
EaeI YGGCCR 5 cut(s) 69, 389, 418, 520, 1331
EagI CGGCCG 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 1006, 1386
EarI CTCTTC 2 cut(s) 1006, 1386
EciI GGCGGA 1 cut(s) 968
EclXI CGGCCG 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 1358
Eco52I CGGCCG 1 cut(s) 418
EcoO109I RGGNCCY 1 cut(s) 1396
EcoRII CCWGG 4 cut(s) 289, 1210, 1353, 1360
EcoT14I CCWWGG 1 cut(s) 523
EcoT22I ATGCAT 2 cut(s) 887, 1238
ErhI CCWWGG 1 cut(s) 523
FaeI CATG 8 cut(s) 19, 598, 651, 885, 1106, 1163, 1168, 1338
FaqI GGGAC 3 cut(s) 269, 443, 570
FatI CATG 8 cut(s) 15, 594, 647, 881, 1102, 1159, 1164, 1334
FauI CCCGC 1 cut(s) 260
FbaI TGATCA 1 cut(s) 867
Fnu4HI GCNGC 5 cut(s) 94, 310, 673, 1191, 1318
FokI GGATG 8 cut(s) 365, 596, 645, 806, 836, 859, 1060, 1093
Fsp4HI GCNGC 5 cut(s) 94, 310, 673, 1191, 1318
GlaI GCGC 1 cut(s) 142
GluI GCNGC 5 cut(s) 94, 310, 673, 1191, 1318
GsaI CCCAGC 1 cut(s) 1308
HaeIII GGCC 6 cut(s) 71, 391, 420, 522, 1333, 1398
HapII CCGG 1 cut(s) 392
HhaI GCGC 1 cut(s) 143
Hin1II CATG 8 cut(s) 19, 598, 651, 885, 1106, 1163, 1168, 1338
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HpaI GTTAAC 1 cut(s) 303
HpaII CCGG 1 cut(s) 392
HphI GGTGA 5 cut(s) 52, 313, 406, 512, 539
Hpy166II GTNNAC 2 cut(s) 303, 1224
Hpy188III TCNNGA 8 cut(s) 227, 374, 613, 844, 865, 984, 1160, 1388
Hpy8I GTNNAC 2 cut(s) 303, 1224
Hpy99I CGWCG 1 cut(s) 415
HpyAV CCTTC 6 cut(s) 257, 416, 420, 654, 1142, 1261
HpyCH4III ACNGT 3 cut(s) 561, 579, 1284
HpyCH4IV ACGT 3 cut(s) 486, 1038, 1245
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 908, 1136, 1268
HpyF3I CTNAG 7 cut(s) 24, 33, 216, 245, 372, 1173, 1194
HpySE526I ACGT 3 cut(s) 486, 1038, 1245
Hsp92II CATG 8 cut(s) 19, 598, 651, 885, 1106, 1163, 1168, 1338
HspAI GCGC 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 867
KspAI GTTAAC 1 cut(s) 303
Kzo9I GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
LmnI GCTCC 2 cut(s) 918, 1444
Lsp1109I GCAGC 3 cut(s) 321, 684, 1202
LweI GCATC 2 cut(s) 343, 837
MaeII ACGT 3 cut(s) 486, 1038, 1245
MaeIII GTNAC 6 cut(s) 270, 500, 712, 1180, 1246, 1369
MalI GATC 6 cut(s) 587, 737, 869, 1099, 1122, 1218
MbiI CCGCTC 1 cut(s) 816
MboI GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
MboII GAAGA 5 cut(s) 353, 459, 1023, 1285, 1403
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 1 cut(s) 1097
MhlI GDGCHC 2 cut(s) 1433, 1449
MlsI TGGCCA 2 cut(s) 71, 522
MluNI TGGCCA 2 cut(s) 71, 522
MlyI GAGTC 3 cut(s) 4, 256, 968
MmeI TCCRAC 2 cut(s) 645, 1179
Mox20I TGGCCA 2 cut(s) 71, 522
Mph1103I ATGCAT 2 cut(s) 887, 1238
MroXI GAANNNNTTC 1 cut(s) 405
MscI TGGCCA 2 cut(s) 71, 522
MseI TTAA 3 cut(s) 302, 563, 636
MslI CAYNNNNRTG 2 cut(s) 351, 1037
Msp20I TGGCCA 2 cut(s) 71, 522
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 4 cut(s) 291, 1212, 1355, 1362
MunI CAATTG 1 cut(s) 1404
Mva1269I GAATGC 2 cut(s) 118, 1132
MvaI CCWGG 4 cut(s) 291, 1212, 1355, 1362
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 4 cut(s) 164, 908, 1136, 1268
NdeII GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
NlaIII CATG 8 cut(s) 19, 598, 651, 885, 1106, 1163, 1168, 1338
NlaIV GGNNCC 5 cut(s) 472, 511, 1099, 1326, 1359
NmeAIII GCCGAG 2 cut(s) 446, 1309
NmuCI GTSAC 4 cut(s) 270, 500, 712, 1180
NsiI ATGCAT 2 cut(s) 887, 1238
NspI RCATGY 1 cut(s) 885
NspV TTCGAA 1 cut(s) 991
PagI TCATGA 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 1 cut(s) 1247
PctI GAATGC 2 cut(s) 118, 1132
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 7 cut(s) 450, 746, 936, 980, 993, 1141, 1207
Pfl23II CGTACG 1 cut(s) 414
PkrI GCNGC 5 cut(s) 95, 311, 674, 1192, 1319
PleI GAGTC 3 cut(s) 4, 255, 968
PpsI GAGTC 3 cut(s) 4, 255, 968
Ppu21I YACGTR 1 cut(s) 1039
PshAI GACNNNNGTC 1 cut(s) 1356
Psp1406I AACGTT 1 cut(s) 1245
Psp6I CCWGG 4 cut(s) 289, 1210, 1353, 1360
PspFI CCCAGC 1 cut(s) 1304
PspGI CCWGG 4 cut(s) 289, 1210, 1353, 1360
PspLI CGTACG 1 cut(s) 414
PspN4I GGNNCC 5 cut(s) 472, 511, 1099, 1326, 1359
PspPI GGNCC 2 cut(s) 1358, 1396
PsuI RGATCY 1 cut(s) 1097
RsaI GTAC 5 cut(s) 137, 254, 416, 581, 599
RsaNI GTAC 5 cut(s) 136, 253, 415, 580, 598
RseI CAYNNNNRTG 2 cut(s) 351, 1037
SaqAI TTAA 3 cut(s) 302, 563, 636
SatI GCNGC 5 cut(s) 94, 310, 673, 1191, 1318
Sau3AI GATC 6 cut(s) 585, 735, 867, 1097, 1120, 1216
Sau96I GGNCC 2 cut(s) 1358, 1396
ScaI AGTACT 1 cut(s) 137
SchI GAGTC 3 cut(s) 4, 256, 968
ScrFI CCNGG 4 cut(s) 291, 1212, 1355, 1362
SduI GDGCHC 2 cut(s) 1433, 1449
SfaNI GCATC 2 cut(s) 343, 837
SfuI TTCGAA 1 cut(s) 991
SinI GGWCC 1 cut(s) 1358
SmiMI CAYNNNNRTG 2 cut(s) 351, 1037
SsiI CCGC 5 cut(s) 94, 267, 816, 953, 1317
StyD4I CCNGG 4 cut(s) 289, 1210, 1353, 1360
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 3 cut(s) 561, 579, 1284
TaiI ACGT 3 cut(s) 489, 1041, 1248
TaqI TCGA 6 cut(s) 132, 516, 588, 763, 991, 1241
TatI WGTACW 3 cut(s) 135, 252, 597
TauI GCSGC 2 cut(s) 96, 1320
TfiI GAWTC 7 cut(s) 450, 746, 936, 980, 993, 1141, 1207
Tru1I TTAA 3 cut(s) 302, 563, 636
Tru9I TTAA 3 cut(s) 302, 563, 636
TscAI CASTG 7 cut(s) 487, 717, 904, 909, 1263, 1300, 1454
TseFI GTSAC 4 cut(s) 270, 500, 712, 1180
TseI GCWGC 3 cut(s) 309, 672, 1190
Tsp45I GTSAC 4 cut(s) 270, 500, 712, 1180
TspDTI ATGAA 6 cut(s) 32, 442, 636, 647, 1070, 1079
TspGWI ACGGA 3 cut(s) 399, 1110, 1209
TspRI CASTG 7 cut(s) 487, 717, 904, 909, 1263, 1300, 1454
VpaK11BI GGWCC 1 cut(s) 1358
XapI RAATTY 1 cut(s) 545
XceI RCATGY 1 cut(s) 885
XcmI CCANNNNNNNNNTGG 2 cut(s) 628, 1109
XmnI GAANNNNTTC 1 cut(s) 405
ZrmI AGTACT 1 cut(s) 137
Zsp2I ATGCAT 2 cut(s) 887, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.