Rh7BG162400

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008948
Physical Location & Seq
Reverse (-)
12540626 .. 12545366
4741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG162400.1

Sequence Viewer

Length: 1404 bp
ATGGCTATGGTGGTACTACTGGTTCTGGTTTTATTGTGCACAAACGGAGTTGAAAGCCGGAAGGCTAAGAATGTGGACAGCTTTGAGTACTCAGCCATAAGTTGCAGAGCCCAAAGTGCATCACTGGCAGATTTTGGAGGAGTTGGTGATGGTATCACATCAAACACCAAGGCTTTTCAGGCAGCAATTCAGCATCTGAGTCAATATGACTCACAAGGTGGATCCCAACTTTTTGTTCCTGCTGGGAAATGGTTAACTGGGAGCTTCAACCTCACCAGCCATTTCACTCTCTATCTCCACAAGGATGCTGTTCTTCTTGCCTCTCAGGATGAGAATGAATGGCCAGTGATTGATCCACTTCCATCGTACGGTAGAGGAAGGGATACTGCTGGAGGAAGATTCATCAGTCTGATATTTGGAACCAACCTCACTGATGTTGTAGTAACAGGGGGAAATGGCACAATTGATGGTCAAGGTGAACTATGGTGGCAAAAATTCCACAAGGGGGAGTTAAAGTACACTCGGCCTTACCTGATTGAAATCATGTTCTCAGAAAATATCCAAATTTCGAACCTTACTTTGGTTAATTCTCCATCATGGAATGTTCATCCTGTTTACAGCAGCAATGTTCTAGTTCAAGGCCTTACAATCCTTGCACCAGTAACGTCTCCAAACACAGATGGGATCAACCCCGACTCTTGCACAAATACTCGAATTGAGGACTGTTACATTGTCTCTGGAGATGATTGTATAGCCGTAAAGAGTGGTTGGGATGAGTATGGTATTGCTTTTGGGATGCCAACCAAAGCGCTTGTGATCAGACGTTTGACATGCATTTCTCCATTTAGTGCTGTGATTGCACTCGGAAGTGAGATGTCTGGTGGGATCCAAGATGTCAGGGCTGAGGATATCCTTGCCATTAACACGGAATCAGGAGTCAGAATCAAAACTGCTGTAGGAAGAGGAGGCTTTGTGAAAGACATATATGTCAGAGGGATGACTATGAAAACCATGAAGTATGTATTTTGGATTGCAGGAGACTATGGATCACATGCAGATGATGGCTATGATCCTAATGCGCTTCCAGTGATCCAGAACATAAATTATCATGATATGGTGGCTGAGAATGTAACAATGGCAGGTAAACTGGGGGGAATAACTGGAGATCCATTTACTGGGATTTGCATATCTAATGTTACAATTACAATGGCAAAGAAGGGAAAGAAAGTACCTTGGAACTGCACTGACGTTGCTGGGATTTCAAGCGGAGTGGTTCCTCAGCCGTGTGAGCTTCTAGCAGACCAGGGTCCGGAGAAGATCGGGGCATGTGATTTCCCAGAAGAAAGTCTTCCGGTTGATAATATGCAACTTCAGCTCTGCTGTTACAGAGGAAAGTACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

467

Amino Acids

50.45

Weight (kDa)

5.09

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 138 - 417 1.3e-39 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 986
Acc36I ACCTGC 1 cut(s) 1130
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccIII TCCGGA 1 cut(s) 1309
AciI CCGC 1 cut(s) 1266
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 2 cut(s) 494, 564
AcuI CTGAAG 1 cut(s) 1355
AdeI CACNNNGTG 1 cut(s) 218
AfaI GTAC 6 cut(s) 15, 89, 368, 518, 1230, 1397
AfeI AGCGCT 1 cut(s) 810
AfiI CCNNNNNNNGG 5 cut(s) 368, 505, 580, 1175, 1309
AgsI TTSAA 5 cut(s) 53, 268, 539, 638, 1263
AjnI CCWGG 1 cut(s) 1302
AjuI GAANNNNNNNTTGG 4 cut(s) 219, 251, 563, 595
AluBI AGCT 4 cut(s) 81, 264, 1291, 1375
AluI AGCT 4 cut(s) 81, 264, 1291, 1375
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 3 cut(s) 672, 739, 1032
Alw44I GTGCAC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 196
Aor13HI TCCGGA 1 cut(s) 1309
Aor51HI AGCGCT 1 cut(s) 810
AoxI GGCC 3 cut(s) 341, 524, 640
ApaLI GTGCAC 1 cut(s) 37
ApeKI GCWGC 2 cut(s) 182, 621
ApoI RAATTY 2 cut(s) 494, 564
Asp700I GAANNNNTTC 1 cut(s) 1347
AspLEI GCGC 2 cut(s) 811, 1081
AspS9I GGNCC 1 cut(s) 1307
AsuHPI GGTGA 3 cut(s) 158, 265, 488
AsuII TTCGAA 1 cut(s) 569
AvaII GGWCC 1 cut(s) 1307
BaeGI GKGCMC 1 cut(s) 41
BalI TGGCCA 1 cut(s) 343
BamHI GGATCC 2 cut(s) 221, 885
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 1340
Bbv12I GWGCWC 1 cut(s) 41
BbvCI CCTCAGC 2 cut(s) 903, 1278
BbvI GCAGC 2 cut(s) 194, 633
BccI CCATC 6 cut(s) 143, 370, 461, 601, 674, 1055
BceAI ACGGC 2 cut(s) 740, 1267
BciT130I CCWGG 1 cut(s) 1304
BciVI GTATCC 1 cut(s) 376
BclI TGATCA 1 cut(s) 816
BcoDI GTCTC 3 cut(s) 672, 739, 1032
BfaI CTAG 2 cut(s) 632, 1295
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 1 cut(s) 812
BfuAI ACCTGC 1 cut(s) 1130
BfuI GTATCC 1 cut(s) 376
BisI GCNGC 2 cut(s) 183, 622
BlsI GCNGC 2 cut(s) 184, 623
BmcAI AGTACT 2 cut(s) 89, 1397
Bme1390I CCNGG 1 cut(s) 1304
Bme18I GGWCC 1 cut(s) 1307
BmgT120I GGNCC 1 cut(s) 1307
BmiI GGNNCC 5 cut(s) 223, 421, 887, 1275, 1308
BmrFI CCNGG 1 cut(s) 1304
BmrI ACTGGG 3 cut(s) 267, 1157, 1185
BmsI GCATC 4 cut(s) 128, 202, 295, 786
BmuI ACTGGG 3 cut(s) 267, 1157, 1185
BoxI GACNNNNGTC 1 cut(s) 1305
BpiI GAAGAC 1 cut(s) 1340
BpmI CTGGAG 3 cut(s) 411, 759, 1182
Bpu10I CCTNAGC 2 cut(s) 903, 1278
Bpu14I TTCGAA 1 cut(s) 569
BsaBI GATNNNNATC 1 cut(s) 539
BsaJI CCNNGG 3 cut(s) 168, 1232, 1303
BsaWI WCCGGW 2 cut(s) 1309, 1351
BsaXI ACNNNNNCTCC 4 cut(s) 500, 530, 957, 987
Bsc4I CCNNNNNNNGG 5 cut(s) 368, 505, 580, 1175, 1309
Bse1I ACTGG 9 cut(s) 24, 129, 262, 344, 659, 1085, 1152, 1165, 1180
Bse3DI GCAATG 1 cut(s) 631
Bse8I GATNNNNATC 1 cut(s) 539
BseAI TCCGGA 1 cut(s) 1309
BseBI CCWGG 1 cut(s) 1304
BseDI CCNNGG 3 cut(s) 168, 1232, 1303
BseGI GGATG 6 cut(s) 310, 334, 607, 778, 801, 1002
BseJI GATNNNNATC 1 cut(s) 539
BseLI CCNNNNNNNGG 5 cut(s) 368, 505, 580, 1175, 1309
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 7 cut(s) 105, 188, 338, 564, 894, 1113, 1292
BseNI ACTGG 9 cut(s) 24, 129, 262, 344, 659, 1085, 1152, 1165, 1180
BseRI GAGGAG 2 cut(s) 153, 978
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 2 cut(s) 194, 633
BseYI CCCAGC 2 cut(s) 242, 1253
BsgI GTGCAG 1 cut(s) 1225
BshFI GGCC 3 cut(s) 343, 526, 642
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 3 cut(s) 58, 1310, 1352
BsiWI CGTACG 1 cut(s) 366
BslI CCNNNNNNNGG 5 cut(s) 368, 505, 580, 1175, 1309
BsmAI GTCTC 3 cut(s) 672, 739, 1032
BsmBI CGTCTC 1 cut(s) 672
BsnI GGCC 3 cut(s) 343, 526, 642
Bsp119I TTCGAA 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 41, 112
Bsp13I TCCGGA 1 cut(s) 1309
BspACI CCGC 1 cut(s) 1266
BspANI GGCC 3 cut(s) 343, 526, 642
BspCNI CTCAG 7 cut(s) 104, 189, 337, 563, 895, 1114, 1291
BspEI TCCGGA 1 cut(s) 1309
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 5 cut(s) 223, 421, 887, 1275, 1308
BspMI ACCTGC 1 cut(s) 1130
BspT104I TTCGAA 1 cut(s) 569
BsrDI GCAATG 1 cut(s) 631
BsrI ACTGG 9 cut(s) 24, 129, 262, 344, 659, 1085, 1152, 1165, 1180
BssECI CCNNGG 3 cut(s) 168, 1232, 1303
BssT1I CCWWGG 2 cut(s) 168, 1232
Bst2UI CCWGG 1 cut(s) 1304
Bst4CI ACNGT 2 cut(s) 371, 725
Bst6I CTCTTC 1 cut(s) 955
BstBI TTCGAA 1 cut(s) 569
BstDEI CTNAG 8 cut(s) 66, 91, 197, 324, 550, 903, 1122, 1278
BstF5I GGATG 6 cut(s) 310, 334, 607, 778, 801, 1002
BstH2I RGCGCY 1 cut(s) 812
BstHHI GCGC 2 cut(s) 811, 1081
BstMAI GTCTC 3 cut(s) 672, 739, 1032
BstMWI GCNNNNNNNGC 6 cut(s) 116, 125, 179, 857, 1288, 1372
BstNI CCWGG 1 cut(s) 1304
BstNSI RCATGY 3 cut(s) 834, 1055, 1329
BstPAI GACNNNNGTC 1 cut(s) 1305
BstSCI CCNGG 1 cut(s) 1302
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 1 cut(s) 41
BstV1I GCAGC 2 cut(s) 194, 633
BstV2I GAAGAC 1 cut(s) 1340
BstX2I RGATCY 3 cut(s) 221, 885, 1165
BstYI RGATCY 3 cut(s) 221, 885, 1165
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 3 cut(s) 343, 526, 642
BtsCI GGATG 6 cut(s) 310, 334, 607, 778, 801, 1002
BtsIMutI CAGTG 5 cut(s) 122, 351, 429, 1092, 1242
BveI ACCTGC 1 cut(s) 1130
CaiI CAGNNNCTG 1 cut(s) 196
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 2 cut(s) 811, 1081
Cfr13I GGNCC 1 cut(s) 1307
Csp6I GTAC 6 cut(s) 14, 88, 367, 517, 1229, 1396
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviAII CATG 7 cut(s) 544, 597, 831, 1012, 1052, 1109, 1326
CviQI GTAC 6 cut(s) 14, 88, 367, 517, 1229, 1396
DdeI CTNAG 8 cut(s) 66, 91, 197, 324, 550, 903, 1122, 1278
DraIII CACNNNGTG 1 cut(s) 218
DrdI GACNNNNNNGTC 1 cut(s) 986
DseDI GACNNNNNNGTC 1 cut(s) 986
EaeI YGGCCR 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 955
EarI CTCTTC 1 cut(s) 955
Eco130I CCWWGG 2 cut(s) 168, 1232
Eco147I AGGCCT 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 112
Eco32I GATATC 1 cut(s) 910
Eco47I GGWCC 1 cut(s) 1307
Eco47III AGCGCT 1 cut(s) 810
Eco57I CTGAAG 1 cut(s) 1355
EcoRII CCWGG 1 cut(s) 1302
EcoRV GATATC 1 cut(s) 910
EcoT14I CCWWGG 2 cut(s) 168, 1232
EcoT22I ATGCAT 1 cut(s) 836
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 2 cut(s) 168, 1232
Esp3I CGTCTC 1 cut(s) 672
FaeI CATG 7 cut(s) 547, 600, 834, 1015, 1055, 1112, 1329
FalI AAGNNNNNCTT 2 cut(s) 1332, 1364
FatI CATG 7 cut(s) 543, 596, 830, 1011, 1051, 1108, 1325
FbaI TGATCA 1 cut(s) 816
Fnu4HI GCNGC 2 cut(s) 183, 622
FokI GGATG 6 cut(s) 317, 341, 594, 785, 808, 1009
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 2 cut(s) 183, 622
FspBI CTAG 2 cut(s) 632, 1295
GlaI GCGC 2 cut(s) 810, 1080
GluI GCNGC 2 cut(s) 183, 622
GsaI CCCAGC 2 cut(s) 246, 1257
GsuI CTGGAG 3 cut(s) 411, 759, 1182
HaeII RGCGCY 1 cut(s) 812
HaeIII GGCC 3 cut(s) 343, 526, 642
HapII CCGG 3 cut(s) 58, 1310, 1352
HhaI GCGC 2 cut(s) 811, 1081
Hin1II CATG 7 cut(s) 547, 600, 834, 1015, 1055, 1112, 1329
Hin6I GCGC 2 cut(s) 809, 1079
HinP1I GCGC 2 cut(s) 809, 1079
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HinfI GANTC 7 cut(s) 199, 209, 399, 695, 929, 936, 942
HpaI GTTAAC 1 cut(s) 255
HpaII CCGG 3 cut(s) 58, 1310, 1352
HphI GGTGA 3 cut(s) 158, 265, 488
Hpy166II GTNNAC 7 cut(s) 39, 76, 255, 479, 519, 616, 1145
Hpy188I TCNGA 7 cut(s) 198, 411, 553, 821, 866, 941, 992
Hpy188III TCNNGA 6 cut(s) 326, 738, 933, 1093, 1109, 1310
Hpy8I GTNNAC 7 cut(s) 39, 76, 255, 479, 519, 616, 1145
HpyAV CCTTC 3 cut(s) 55, 372, 1210
HpyCH4III ACNGT 2 cut(s) 371, 725
HpyCH4IV ACGT 3 cut(s) 665, 823, 1248
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 125, 179, 857, 1288, 1372
HpyF3I CTNAG 8 cut(s) 66, 91, 197, 324, 550, 903, 1122, 1278
HpySE526I ACGT 3 cut(s) 665, 823, 1248
Hsp92II CATG 7 cut(s) 547, 600, 834, 1015, 1055, 1112, 1329
HspAI GCGC 2 cut(s) 809, 1079
Kpn2I TCCGGA 1 cut(s) 1309
Ksp22I TGATCA 1 cut(s) 816
KspAI GTTAAC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 2 cut(s) 194, 633
LweI GCATC 4 cut(s) 128, 202, 295, 786
MaeI CTAG 2 cut(s) 632, 1295
MaeII ACGT 3 cut(s) 665, 823, 1248
MaeIII GTNAC 6 cut(s) 442, 661, 725, 1129, 1195, 1382
MboII GAAGA 6 cut(s) 305, 408, 972, 1327, 1340, 1352
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 3 cut(s) 221, 885, 1165
MhlI GDGCHC 2 cut(s) 41, 112
MlsI TGGCCA 1 cut(s) 343
MluCI AATT 8 cut(s) 186, 462, 494, 564, 586, 714, 1102, 1200
MluNI TGGCCA 1 cut(s) 343
MlyI GAGTC 4 cut(s) 203, 208, 689, 945
Mox20I TGGCCA 1 cut(s) 343
Mph1103I ATGCAT 1 cut(s) 836
MroI TCCGGA 1 cut(s) 1309
MroXI GAANNNNTTC 1 cut(s) 1347
MscI TGGCCA 1 cut(s) 343
MseI TTAA 4 cut(s) 254, 512, 585, 921
MslI CAYNNNNRTG 2 cut(s) 303, 1056
Msp20I TGGCCA 1 cut(s) 343
MspI CCGG 3 cut(s) 58, 1310, 1352
MspR9I CCNGG 1 cut(s) 1304
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 1304
MwoI GCNNNNNNNGC 6 cut(s) 116, 125, 179, 857, 1288, 1372
NlaIII CATG 7 cut(s) 547, 600, 834, 1015, 1055, 1112, 1329
NlaIV GGNNCC 5 cut(s) 223, 421, 887, 1275, 1308
NmeAIII GCCGAG 1 cut(s) 502
NsiI ATGCAT 1 cut(s) 836
NspI RCATGY 3 cut(s) 834, 1055, 1329
NspV TTCGAA 1 cut(s) 569
PagI TCATGA 1 cut(s) 1108
PceI AGGCCT 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 1347
PfeI GAWTC 3 cut(s) 399, 929, 942
Pfl23II CGTACG 1 cut(s) 366
PflMI CCANNNNNTGG 1 cut(s) 1175
PkrI GCNGC 2 cut(s) 184, 623
PleI GAGTC 4 cut(s) 203, 207, 689, 944
PpsI GAGTC 4 cut(s) 203, 207, 689, 944
PshAI GACNNNNGTC 1 cut(s) 1305
Psp6I CCWGG 1 cut(s) 1302
PspFI CCCAGC 2 cut(s) 242, 1253
PspGI CCWGG 1 cut(s) 1302
PspLI CGTACG 1 cut(s) 366
PspN4I GGNNCC 5 cut(s) 223, 421, 887, 1275, 1308
PspPI GGNCC 1 cut(s) 1307
PsrI GAACNNNNNNTAC 1 cut(s) 38
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 3 cut(s) 221, 885, 1165
RsaI GTAC 6 cut(s) 15, 89, 368, 518, 1230, 1397
RsaNI GTAC 6 cut(s) 14, 88, 367, 517, 1229, 1396
RseI CAYNNNNRTG 2 cut(s) 303, 1056
SaqAI TTAA 4 cut(s) 254, 512, 585, 921
SatI GCNGC 2 cut(s) 183, 622
Sau96I GGNCC 1 cut(s) 1307
ScaI AGTACT 2 cut(s) 89, 1397
SchI GAGTC 4 cut(s) 203, 208, 689, 945
ScrFI CCNGG 1 cut(s) 1304
SduI GDGCHC 2 cut(s) 41, 112
SfaNI GCATC 4 cut(s) 128, 202, 295, 786
SfcI CTRYAG 1 cut(s) 954
SfuI TTCGAA 1 cut(s) 569
SinI GGWCC 1 cut(s) 1307
SmiMI CAYNNNNRTG 2 cut(s) 303, 1056
Sse9I AATT 8 cut(s) 186, 462, 494, 564, 586, 714, 1102, 1200
SseBI AGGCCT 1 cut(s) 642
SsiI CCGC 1 cut(s) 1266
SspMI CTAG 2 cut(s) 632, 1295
StuI AGGCCT 1 cut(s) 642
StyD4I CCNGG 1 cut(s) 1302
StyI CCWWGG 2 cut(s) 168, 1232
TaaI ACNGT 2 cut(s) 371, 725
TaiI ACGT 3 cut(s) 668, 826, 1251
TaqI TCGA 2 cut(s) 569, 712
TasI AATT 8 cut(s) 186, 462, 494, 564, 586, 714, 1102, 1200
TatI WGTACW 3 cut(s) 87, 516, 1395
TfiI GAWTC 3 cut(s) 399, 929, 942
Tru1I TTAA 4 cut(s) 254, 512, 585, 921
Tru9I TTAA 4 cut(s) 254, 512, 585, 921
TscAI CASTG 5 cut(s) 129, 351, 436, 1092, 1249
TseI GCWGC 2 cut(s) 182, 621
TspDTI ATGAA 5 cut(s) 351, 391, 596, 1019, 1028
TspGWI ACGGA 2 cut(s) 60, 941
TspRI CASTG 5 cut(s) 129, 351, 436, 1092, 1249
Van91I CCANNNNNTGG 1 cut(s) 1175
VneI GTGCAC 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 1307
XapI RAATTY 2 cut(s) 494, 564
XceI RCATGY 3 cut(s) 834, 1055, 1329
XmnI GAANNNNTTC 1 cut(s) 1347
XspI CTAG 2 cut(s) 632, 1295
ZrmI AGTACT 2 cut(s) 89, 1397
Zsp2I ATGCAT 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.