AT4G23950

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013940
Physical Location & Seq
Forward (+)
12442047 .. 12444547
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23950.2

Sequence Viewer

Length: 1686 bp
ATGGCGAGGCGCGGAAGCTGTTCGACGATATGCCTGAATGAAAAATTGCAAAGGTTTCGAATTGTCAGAATCTCCGACAAGGCTGATAATTTTAATTCCAGAAGCGGCAGCTTCTTCGAGCGTTCTATCTCTCTCGTTCTCTTACTCTGGTGCTTCCTCTTCCTCGTCTACTCCAAGCTTGGCCAAAGCCATGATGATTATGGTAATGCAGATAGAATTGGGAATTACACTGATGGATCAGTCTCAAAGACACTGAATTCCACGAGTTCTGTCTTCCCTCAGGCCACTGAGAAAGAAAACAACTTTTGTCTCCTGAGAAAAGGCCAGTTACAAGATGTGTATGAGCATGTTCTTGTGAACAACGCTTTGTTGATCTGCAAAGTTGTGTTGCCAGAACGAAGAATAAGCAAGAAAACTCTGGAAGCTCGTGATCCTAGATATGTCAATCTTGAAGACAAGAGTCTCAAAGTTAATGGCTCATCACAGCTTGTGAATAATGGGACTCGTTATAGGTTAGAACCAGATGGAAATGGGTATAACTACGCTTCTGCTATGAAGGGTGCTAAAGTTGTGGATCACAACAAGGAAGCAAAAGGAGCTAGCAATGTGCTTGGCAAAGATCATGATAAGTATCTAAGAAATCCCTGTTCAGTGTCAGATAAGTATGTTGTGATTGAGCTCGCCGAGGAGACACTGGTTGATACGGTGAGAATTGCAAATTTTGAGCATTACTCTTCGAATCCCAAAGAGTTTAGCTTGTCAGGGAGTCTGAGTTTCCCTAGTGATATGTGGACACCTGCTGGGAGTTTTGCGGCTGCAAACGTTAAGCAGATCCAGTCATTCCGGCTCCCGGAACCTAAATGGTTGAGATATCTGAAACTTAATTTAGTTAGTCACTATGGGTCTGAGTTTTACTGTACTCTGAGTGTTGTGGAGGTATTTGGCATTGATGCTCTTGAGCAAATGCTTGAAGATCTGTTTGTTCCATCAGAGACTCCTCCCAGTAAACCAGCTATGGTGGAACTAAAAACTGCAGATGAAAAACAAGATGGGGAAATAAAAAGCAATAGGACAGATCAGATTGGCAAAGAAACTGAAGCTCAGAAGAAGAAAGATGATGTTGTGAAGACGATTAATATCATAGGAGACAAAAAGTATGAAGTCAAGGAGAAGCATAACGTTTTGAAAGTGATGATGCAGAAAGTGAAATTGATTGAGATGAATTTATCGCTTCTTGAGGATTCTGTAAAGAAAATGAATGACAAACAACCAGAGGTTAGCTTGGAGATGAAGAAAACCCTAGTACTTGTGGAGAAAAGCAAAGCTGACATCAGAGAGATCACAGAATGGAAAGGGAAAATGGAAAAGGAACTTAGAGACCTAGAGCTATGGAAAACTCTTGTAGCTTCCCGTGTAGAATCATTGGCTAGAGGAAACTCTGCATTGAGATTGGATGTAGAGAAGATTGTGAAAGAACAAGCTAATCTAGAGAGTAAAGAGCTGGGAGTTCTATTGATAAGCCTCTTTTTCGTGGTCTTAGCAACGATTCGGTTAGTCTCAACACGACTCTGGGCATTTCTGGGGATGTCTATTACGGATAAAGCTCGATCACTATGGCCGGATAGTGGCTGGGTCATGATACTGCTAAGCAGCAGCATCATGATTTTCATTCATTTACTCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0043621 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sad1_UNC

Protein Analysis

561

Amino Acids

63.59

Weight (kDa)

8.74

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 193 - 315 7.8e-31 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23950 AT4G23950
fragaria_vesca FvH4_5g04871 FvH4_5g04871
malus_domestica MD06G1109600.v1.1 MD14G1130600.v1.1
prunus_persica Prupe.5G122600_v2.0.a1 Prupe.5G122600_v2.0.a1
pyrus_communis pycom06g10320
rosa_chinensis RchiOBHm_Chr7g0194211
rosa_laevigata RLG00000004210
rosa_multiflora Rmu_sc0018372.1_g000001
rosa_rugosa Rorug07G0014400
rosa_samantha Rh7AG141600 Rh7BG142100 Rh7DG143100
rosa_wichuraiana Rw7G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 805
Acc36I ACCTGC 1 cut(s) 805
AccI GTMKAC 1 cut(s) 168
AccII CGCG 1 cut(s) 12
AciI CCGC 3 cut(s) 12, 105, 812
AclI AACGTT 2 cut(s) 822, 1179
AclWI GGATC 4 cut(s) 244, 425, 582, 826
AcoI YGGCCR 2 cut(s) 181, 1616
AcsI RAATTY 3 cut(s) 256, 718, 1222
AcuI CTGAAG 1 cut(s) 1116
AfaI GTAC 2 cut(s) 919, 1305
AfiI CCNNNNNNNGG 2 cut(s) 850, 1625
AgsI TTSAA 3 cut(s) 452, 971, 1186
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 8 cut(s) 247, 314, 467, 683, 986, 1140, 1371, 1561
AlwI GGATC 4 cut(s) 244, 425, 582, 826
AoxI GGCC 4 cut(s) 181, 282, 322, 1616
ApeKI GCWGC 4 cut(s) 108, 815, 1650, 1653
ApoI RAATTY 3 cut(s) 256, 718, 1222
AseI ATTAAT 1 cut(s) 1134
Asp700I GAANNNNTTC 1 cut(s) 19
AspLEI GCGC 1 cut(s) 12
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 1 cut(s) 718
AsuII TTCGAA 2 cut(s) 58, 737
AsuNHI GCTAGC 1 cut(s) 599
AxyI CCTNAGG 1 cut(s) 279
BalI TGGCCA 1 cut(s) 183
BanII GRGCYC 1 cut(s) 681
BauI CACGAG 2 cut(s) 262, 426
BbsI GAAGAC 3 cut(s) 265, 459, 1133
Bbv12I GWGCWC 1 cut(s) 681
BbvI GCAGC 4 cut(s) 120, 802, 1662, 1665
BccI CCATC 4 cut(s) 227, 518, 994, 1043
BcgI CGANNNNNNTGC 4 cut(s) 38, 72, 97, 131
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 8 cut(s) 247, 314, 467, 683, 986, 1140, 1371, 1561
BfaI CTAG 7 cut(s) 435, 600, 780, 1301, 1382, 1428, 1487
BfmI CTRYAG 1 cut(s) 1032
BfuAI ACCTGC 1 cut(s) 805
BglII AGATCT 1 cut(s) 973
BisI GCNGC 6 cut(s) 106, 109, 813, 816, 1651, 1654
BlpI GCTNAGC 1 cut(s) 1646
BlsI GCNGC 6 cut(s) 107, 110, 814, 817, 1652, 1655
BmcAI AGTACT 1 cut(s) 1305
Bme1390I CCNGG 1 cut(s) 851
BmiI GGNNCC 2 cut(s) 848, 855
BmrFI CCNGG 1 cut(s) 851
BmrI ACTGGG 1 cut(s) 996
BmsI GCATC 3 cut(s) 940, 1185, 1665
BmtI GCTAGC 1 cut(s) 603
BmuI ACTGGG 1 cut(s) 996
BoxI GACNNNNGTC 1 cut(s) 459
BpiI GAAGAC 3 cut(s) 265, 459, 1133
BplI GAGNNNNNCTC 2 cut(s) 716, 748
Bpu1102I GCTNAGC 1 cut(s) 1646
Bpu14I TTCGAA 2 cut(s) 58, 737
BpuEI CTTGAG 2 cut(s) 977, 1256
BpuMI CCSGG 1 cut(s) 851
BsaBI GATNNNNATC 2 cut(s) 630, 1136
BsaI GGTCTC 1 cut(s) 1371
BsaJI CCNNGG 1 cut(s) 684
BsaXI ACNNNNNCTCC 2 cut(s) 757, 787
Bsc4I CCNNNNNNNGG 2 cut(s) 850, 1625
Bse1I ACTGG 4 cut(s) 325, 699, 835, 1002
Bse21I CCTNAGG 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 610
Bse8I GATNNNNATC 2 cut(s) 630, 1136
BseDI CCNNGG 1 cut(s) 684
BseGI GGATG 2 cut(s) 1459, 1590
BseJI GATNNNNATC 2 cut(s) 630, 1136
BseLI CCNNNNNNNGG 2 cut(s) 850, 1625
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 7 cut(s) 279, 293, 305, 761, 897, 914, 1115
BseNI ACTGG 4 cut(s) 325, 699, 835, 1002
BseRI GAGGAG 2 cut(s) 701, 987
BseXI GCAGC 4 cut(s) 120, 802, 1662, 1665
BseYI CCCAGC 3 cut(s) 800, 1501, 1629
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 183, 284, 324, 1618
BsiHKAI GWGCWC 1 cut(s) 681
BsiSI CCGG 3 cut(s) 844, 851, 1619
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 2 cut(s) 850, 1625
BsmAI GTCTC 8 cut(s) 247, 314, 467, 683, 986, 1140, 1371, 1561
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 4 cut(s) 183, 284, 324, 1618
Bso31I GGTCTC 1 cut(s) 1371
Bsp119I TTCGAA 2 cut(s) 58, 737
Bsp1286I GDGCHC 1 cut(s) 681
Bsp1720I GCTNAGC 1 cut(s) 1646
BspACI CCGC 3 cut(s) 12, 105, 812
BspANI GGCC 4 cut(s) 183, 284, 324, 1618
BspCNI CTCAG 7 cut(s) 280, 292, 306, 762, 898, 915, 1114
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 3 cut(s) 622, 1635, 1659
BspLI GGNNCC 2 cut(s) 848, 855
BspMAI CTGCAG 1 cut(s) 1036
BspMI ACCTGC 1 cut(s) 805
BspOI GCTAGC 1 cut(s) 603
BspPI GGATC 4 cut(s) 244, 425, 582, 826
BspT104I TTCGAA 2 cut(s) 58, 737
BspTNI GGTCTC 1 cut(s) 1371
BsrDI GCAATG 1 cut(s) 610
BsrI ACTGG 4 cut(s) 325, 699, 835, 1002
BssECI CCNNGG 1 cut(s) 684
BssSI CACGAG 2 cut(s) 262, 426
Bst2BI CACGAG 2 cut(s) 262, 426
Bst4CI ACNGT 2 cut(s) 706, 917
Bst6I CTCTTC 2 cut(s) 164, 739
BstBI TTCGAA 2 cut(s) 58, 737
BstC8I GCNNGC 2 cut(s) 601, 681
BstF5I GGATG 2 cut(s) 1459, 1590
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 12
BstMAI GTCTC 8 cut(s) 247, 314, 467, 683, 986, 1140, 1371, 1561
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstNSI RCATGY 1 cut(s) 350
BstPAI GACNNNNGTC 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 849
BstSFI CTRYAG 1 cut(s) 1032
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 4 cut(s) 120, 802, 1662, 1665
BstV2I GAAGAC 3 cut(s) 265, 459, 1133
BstX2I RGATCY 2 cut(s) 831, 973
BstYI RGATCY 2 cut(s) 831, 973
Bsu36I CCTNAGG 1 cut(s) 279
BsuRI GGCC 4 cut(s) 183, 284, 324, 1618
BtsCI GGATG 2 cut(s) 1459, 1590
BtsIMutI CAGTG 5 cut(s) 228, 251, 285, 657, 692
BveI ACCTGC 1 cut(s) 805
Cac8I GCNNGC 2 cut(s) 601, 681
CciI TCATGA 3 cut(s) 622, 1635, 1659
CfoI GCGC 1 cut(s) 12
Csp6I GTAC 2 cut(s) 918, 1304
CviAII CATG 5 cut(s) 191, 347, 623, 1636, 1660
CviQI GTAC 2 cut(s) 918, 1304
EaeI YGGCCR 2 cut(s) 181, 1616
Eam1104I CTCTTC 2 cut(s) 164, 739
EarI CTCTTC 2 cut(s) 164, 739
Ecl136II GAGCTC 1 cut(s) 679
Eco24I GRGCYC 1 cut(s) 681
Eco31I GGTCTC 1 cut(s) 1371
Eco32I GATATC 1 cut(s) 872
Eco53kI GAGCTC 1 cut(s) 679
Eco57I CTGAAG 1 cut(s) 1116
Eco81I CCTNAGG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 679
EcoRI GAATTC 1 cut(s) 256
EcoRV GATATC 1 cut(s) 872
EcoT38I GRGCYC 1 cut(s) 681
FaeI CATG 5 cut(s) 194, 350, 626, 1639, 1663
FaqI GGGAC 1 cut(s) 514
FatI CATG 5 cut(s) 190, 346, 622, 1635, 1659
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 6 cut(s) 106, 109, 813, 816, 1651, 1654
FokI GGATG 2 cut(s) 1466, 1597
FriOI GRGCYC 1 cut(s) 681
Fsp4HI GCNGC 6 cut(s) 106, 109, 813, 816, 1651, 1654
FspBI CTAG 7 cut(s) 435, 600, 780, 1301, 1382, 1428, 1487
GlaI GCGC 1 cut(s) 11
GluI GCNGC 6 cut(s) 106, 109, 813, 816, 1651, 1654
GsaI CCCAGC 3 cut(s) 804, 1505, 1633
HaeIII GGCC 4 cut(s) 183, 284, 324, 1618
HapII CCGG 3 cut(s) 844, 851, 1619
HhaI GCGC 1 cut(s) 12
Hin1II CATG 5 cut(s) 194, 350, 626, 1639, 1663
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 176
HpaII CCGG 3 cut(s) 844, 851, 1619
HphI GGTGA 1 cut(s) 718
Hpy166II GTNNAC 4 cut(s) 169, 358, 792, 1007
Hpy8I GTNNAC 4 cut(s) 169, 358, 792, 1007
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 2 cut(s) 706, 917
HpyCH4IV ACGT 2 cut(s) 822, 1179
HpyCH4V TGCA 8 cut(s) 49, 209, 378, 716, 818, 1034, 1198, 1442
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpySE526I ACGT 2 cut(s) 822, 1179
Hsp92II CATG 5 cut(s) 194, 350, 626, 1639, 1663
HspAI GCGC 1 cut(s) 10
LmnI GCTCC 2 cut(s) 596, 852
Lsp1109I GCAGC 4 cut(s) 120, 802, 1662, 1665
LweI GCATC 3 cut(s) 940, 1185, 1665
MaeI CTAG 7 cut(s) 435, 600, 780, 1301, 1382, 1428, 1487
MaeII ACGT 2 cut(s) 822, 1179
MaeIII GTNAC 2 cut(s) 327, 893
MflI RGATCY 2 cut(s) 831, 973
MhlI GDGCHC 1 cut(s) 681
MlsI TGGCCA 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 183
MlyI GAGTC 5 cut(s) 469, 496, 775, 988, 1560
MmeI TCCRAC 1 cut(s) 99
Mox20I TGGCCA 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 19
MscI TGGCCA 1 cut(s) 183
MseI TTAA 5 cut(s) 93, 471, 825, 882, 1134
Msp20I TGGCCA 1 cut(s) 183
MspI CCGG 3 cut(s) 844, 851, 1619
MspR9I CCNGG 1 cut(s) 851
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 596
NciI CCSGG 1 cut(s) 851
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 5 cut(s) 194, 350, 626, 1639, 1663
NlaIV GGNNCC 2 cut(s) 848, 855
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 1 cut(s) 893
NspI RCATGY 1 cut(s) 350
NspV TTCGAA 2 cut(s) 58, 737
PagI TCATGA 3 cut(s) 622, 1635, 1659
PaqCI CACCTGC 1 cut(s) 805
PdmI GAANNNNTTC 1 cut(s) 19
PfeI GAWTC 5 cut(s) 69, 739, 1241, 1418, 1546
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 6 cut(s) 107, 110, 814, 817, 1652, 1655
PleI GAGTC 5 cut(s) 468, 496, 774, 988, 1560
PpsI GAGTC 5 cut(s) 468, 496, 774, 988, 1560
PshAI GACNNNNGTC 1 cut(s) 459
PshBI ATTAAT 1 cut(s) 1134
Psp124BI GAGCTC 1 cut(s) 681
Psp1406I AACGTT 2 cut(s) 822, 1179
PspFI CCCAGC 3 cut(s) 800, 1501, 1629
PspN4I GGNNCC 2 cut(s) 848, 855
PstI CTGCAG 1 cut(s) 1036
PsuI RGATCY 2 cut(s) 831, 973
RsaI GTAC 2 cut(s) 919, 1305
RsaNI GTAC 2 cut(s) 918, 1304
SacI GAGCTC 1 cut(s) 681
SaqAI TTAA 5 cut(s) 93, 471, 825, 882, 1134
SatI GCNGC 6 cut(s) 106, 109, 813, 816, 1651, 1654
ScaI AGTACT 1 cut(s) 1305
SchI GAGTC 5 cut(s) 469, 496, 775, 988, 1560
ScrFI CCNGG 1 cut(s) 851
SduI GDGCHC 1 cut(s) 681
SfaNI GCATC 3 cut(s) 940, 1185, 1665
SfcI CTRYAG 1 cut(s) 1032
SfuI TTCGAA 2 cut(s) 58, 737
SmlI CTYRAG 2 cut(s) 956, 1235
SmoI CTYRAG 2 cut(s) 956, 1235
SsiI CCGC 3 cut(s) 12, 105, 812
SspMI CTAG 7 cut(s) 435, 600, 780, 1301, 1382, 1428, 1487
SstI GAGCTC 1 cut(s) 681
StyD4I CCNGG 1 cut(s) 849
TaaI ACNGT 2 cut(s) 706, 917
TaiI ACGT 2 cut(s) 825, 1182
TaqI TCGA 5 cut(s) 23, 58, 117, 737, 1606
TatI WGTACW 2 cut(s) 917, 1303
TauI GCSGC 2 cut(s) 108, 815
TfiI GAWTC 5 cut(s) 69, 739, 1241, 1418, 1546
Tru1I TTAA 5 cut(s) 93, 471, 825, 882, 1134
Tru9I TTAA 5 cut(s) 93, 471, 825, 882, 1134
TscAI CASTG 5 cut(s) 235, 258, 292, 657, 699
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 4 cut(s) 108, 815, 1650, 1653
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 9 cut(s) 54, 569, 1053, 1173, 1235, 1271, 1304, 1657, 1661
TspGWI ACGGA 1 cut(s) 1610
TspRI CASTG 5 cut(s) 235, 258, 292, 657, 699
VspI ATTAAT 1 cut(s) 1134
XapI RAATTY 3 cut(s) 256, 718, 1222
XbaI TCTAGA 1 cut(s) 1486
XceI RCATGY 1 cut(s) 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 7 cut(s) 435, 600, 780, 1301, 1382, 1428, 1487
ZrmI AGTACT 1 cut(s) 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.