RLG00000004210

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013940
Physical Location & Seq
Forward (+)
57289327 .. 57292100
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004210

Sequence Viewer

Length: 1893 bp
ATGAAGAAGCCTCGCCACGGTTCCTTACATATCAAAATCCAAAGCAACAATAACTCTGACACCAAGGACAAAGGAGACAAAAACAACAAGAGCAGCAACCACTACAACAACAACAATAAAGGGAAGAGCTTCTATGAGCTCTCTCTGTCTTTGATCTTCTCTCTCTGGTGTCTTGTTGTCTTGTTCTACTCTAAGCTTGGTCTTGGCCATGGCAATGGAGGGAATATTACAGGTCCGAGTAACAGAACTACACATTGTCCTGGTGTTCACAATGGTAAGCATGGTGAGCATGCATATTCATTTGTGGCAAATGGAGGCGAAAATAAGACAAATGGGATGCTTTTAGATTATAATTCGTCTGAAAGTTGTAATGGTACTGCTGTTGGTCACAACTTGCCAGTTTCGAAGTACTCACTGCCGGAGACAAATGGATTAGAAAGGATAGTTTGGAGAGTTTTGGGTTATAGCAGTTTGGTCTGTGAACTCAACCAAGAACAAGAAGAGCATAACAGAAACTGTCCAGGACAATGTGTGAATAGAACTTCTCACTCTACCTATCTCAATCTTGATGAATTTCGAAACGTAACAAAGCAAGAGAAAGACCGTGTAATGCCTAGTCAGCTTGGTAACATTACCCATGGGTTCGAACCTGATGGAACGGAGTACAACTATGCCTCGGAATCCAAGGGGGCAAAGGTGGTGTCTCACAACAAGGAAGCAAAAGGAGCAAGTAACATATTAGGCAGGGATCACGATAAATACCTCAGAAACCCTTGTTCCGTAGAGGGAAAGTTTGTTATAATTGAGCTCGCAGAGGAGACTCTGGTAGATGCTGTCAAAATTGCAAATTTTGAGCACTACTCTTCTAATTTCAAGGAATTTAAATTGTCTGGCAGTTTAAGCTATCCAACAGAAGCATGGTCACCGCTGGGTAGCTTTGTTGCTGCTAATAGTAAGCATGCTCAAACCTTTAAGCTTCCTGAAGCCAAATGGGTGAGATACCTAAAGTTGGATTTACTAAGCCACTACGGAGCAGAGTTTTACTGCACACTTAGTGTTCTAGAGGTGTATGGTATTAATGCAATCGAAAGAATGCTTGAAGATCTCATTGTGGAGTCTGCAGAATCTGCAACCAACAAGTTGCCAGAGCCTAATGCAACTGTAATATCTTCTACAAAGGAAGTGGTTGGTCCAACTGACAGGAAAACAAGCAGCGATAGTCAAAATGGGATTGGTGCAGCTGTAGGGATACAAAGCACTGAAGAAGCGCAACGAGCCAATTCAGATGCTTCCAAGAATTCAGTAACTACTAACAAGATTCCTGATCCGGTTATGACAGTTAGGCAGCAGCCAAATGGAAGAATTCCTGGTGATTCTGTTCTGAAGATTTTGATGCAGAAGGTGCGGTCACTTGAGCTGAACTTGTCCGTGCTGGAGGAGTTTATCAAAGAATTGAACCGAAGACAAGGTGATGTTTTGCCAGAGCTTGGTAAAGAGATATTAAGAATATCATTGCTGTTGGAGCAAAGCAAAATGGAGATTAAAGATCTCATGCAATGGAAGGAAACTATGGAAAAACAAATGAGTGACCTTGAACTATGGAAAGCTGCTGTCTCGTCTCAATTGAATGCATTGGTCAGGGAAAATAACTTGTTGAGATTAGATTTCGAAAAGGTTGTAAATGATCAAGCCAGTCTCGAAAGCAAAGAGCTTGCTATTTTAGCAGTTAGTCTTTTCTTTTTGTGCTTCGCGATCCTCCAGCTAGTTTCGTTTCAAATTTTGACATTTTTCAGAACCTCTCAGCCTGACAACAGATGCAGGAAAAGTAGAGGTTGGGTATTAGTCATTGTTAGCAGCAGTATGACAATATTCATAACTTTGATATATAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sad1_UNC

Protein Analysis

631

Amino Acids

70.36

Weight (kDa)

7.0

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 236 - 358 6.2e-34 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23950 AT4G23950
fragaria_vesca FvH4_5g04871 FvH4_5g04871
malus_domestica MD06G1109600.v1.1 MD14G1130600.v1.1
prunus_persica Prupe.5G122600_v2.0.a1 Prupe.5G122600_v2.0.a1
pyrus_communis pycom06g10320
rosa_chinensis RchiOBHm_Chr7g0194211
rosa_laevigata RLG00000004210
rosa_multiflora Rmu_sc0018372.1_g000001
rosa_rugosa Rorug07G0014400
rosa_samantha Rh7AG141600 Rh7BG142100 Rh7DG143100
rosa_wichuraiana Rw7G012110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 351, 800
AccB7I CCANNNNNTGG 1 cut(s) 1487
AccII CGCG 1 cut(s) 1751
AciI CCGC 2 cut(s) 926, 1405
AclWI GGATC 3 cut(s) 756, 1319, 1747
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 6 cut(s) 572, 847, 878, 1297, 1362, 1776
AcuI CTGAAG 3 cut(s) 1002, 1281, 1403
AdeI CACNNNGTG 1 cut(s) 1055
AfaI GTAC 3 cut(s) 376, 410, 665
AfiI CCNNNNNNNGG 3 cut(s) 17, 1009, 1487
AgsI TTSAA 6 cut(s) 874, 1100, 1456, 1595, 1627, 1775
AjnI CCWGG 3 cut(s) 259, 520, 1366
Alw21I GWGCWC 3 cut(s) 141, 810, 858
Alw26I GTCTC 7 cut(s) 69, 416, 708, 812, 1618, 1623, 1700
AlwI GGATC 3 cut(s) 756, 1319, 1747
AlwNI CAGNNNCTG 2 cut(s) 516, 1127
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 8 cut(s) 93, 944, 1212, 1238, 1345, 1348, 1607, 1854
ApoI RAATTY 6 cut(s) 572, 847, 878, 1297, 1362, 1776
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AseI ATTAAT 1 cut(s) 1077
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 1270
AspS9I GGNCC 2 cut(s) 233, 1190
AsuHPI GGTGA 5 cut(s) 296, 915, 1006, 1382, 1481
AsuII TTCGAA 4 cut(s) 404, 577, 645, 1668
AvaII GGWCC 2 cut(s) 233, 1190
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 207
BanII GRGCYC 2 cut(s) 141, 810
BbsI GAAGAC 1 cut(s) 1468
Bbv12I GWGCWC 3 cut(s) 141, 810, 858
BbvI GCAGC 8 cut(s) 105, 931, 1224, 1250, 1357, 1360, 1594, 1866
BccI CCATC 1 cut(s) 647
BciT130I CCWGG 3 cut(s) 261, 522, 1368
BciVI GTATCC 1 cut(s) 1242
BclI TGATCA 1 cut(s) 1684
BcoDI GTCTC 7 cut(s) 69, 416, 708, 812, 1618, 1623, 1700
BfaI CTAG 4 cut(s) 615, 1061, 1763, 1891
BfmI CTRYAG 2 cut(s) 1119, 1242
BfuI GTATCC 1 cut(s) 1242
BglII AGATCT 2 cut(s) 1102, 1546
BisI GCNGC 8 cut(s) 94, 945, 1213, 1239, 1346, 1349, 1608, 1855
BlsI GCNGC 8 cut(s) 95, 946, 1214, 1240, 1347, 1350, 1609, 1856
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 3 cut(s) 261, 522, 1368
Bme18I GGWCC 2 cut(s) 233, 1190
BmgT120I GGNCC 2 cut(s) 233, 1190
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 3 cut(s) 261, 522, 1368
BmsI GCATC 5 cut(s) 327, 820, 1276, 1383, 1805
BpiI GAAGAC 1 cut(s) 1468
BplI GAGNNNNNCTC 2 cut(s) 845, 877
BpmI CTGGAG 2 cut(s) 1454, 1742
Bpu14I TTCGAA 4 cut(s) 404, 577, 645, 1668
BpuEI CTTGAG 1 cut(s) 1433
BsaJI CCNNGG 6 cut(s) 16, 63, 208, 637, 675, 684
BsaWI WCCGGW 1 cut(s) 1327
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 1009, 1487
Bse1I ACTGG 2 cut(s) 398, 1692
Bse3DI GCAATG 3 cut(s) 220, 1511, 1562
BseBI CCWGG 3 cut(s) 261, 522, 1368
BseDI CCNNGG 6 cut(s) 16, 63, 208, 637, 675, 684
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 3 cut(s) 17, 1009, 1487
BseMI GCAATG 3 cut(s) 220, 1511, 1562
BseMII CTCAG 2 cut(s) 778, 1814
BseNI ACTGG 2 cut(s) 398, 1692
BseRI GAGGAG 2 cut(s) 830, 1451
BseXI GCAGC 8 cut(s) 105, 931, 1224, 1250, 1357, 1360, 1594, 1866
BseYI CCCAGC 1 cut(s) 928
BsgI GTGCAG 2 cut(s) 1030, 1257
Bsh1236I CGCG 1 cut(s) 1751
BshFI GGCC 1 cut(s) 207
BsiHKAI GWGCWC 3 cut(s) 141, 810, 858
BsiSI CCGG 2 cut(s) 419, 1328
BslI CCNNNNNNNGG 3 cut(s) 17, 1009, 1487
BsmAI GTCTC 7 cut(s) 69, 416, 708, 812, 1618, 1623, 1700
BsmBI CGTCTC 1 cut(s) 1623
BsmI GAATGC 2 cut(s) 1098, 1633
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 4 cut(s) 404, 577, 645, 1668
Bsp1286I GDGCHC 3 cut(s) 141, 810, 858
Bsp143I GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
Bsp19I CCATGG 2 cut(s) 208, 637
Bsp68I TCGCGA 1 cut(s) 1751
BspACI CCGC 2 cut(s) 926, 1405
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 2 cut(s) 777, 1813
BspFNI CGCG 1 cut(s) 1751
BspLI GGNNCC 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 1123
BspPI GGATC 3 cut(s) 756, 1319, 1747
BspQI GCTCTTC 2 cut(s) 119, 495
BspT104I TTCGAA 4 cut(s) 404, 577, 645, 1668
BsrDI GCAATG 3 cut(s) 220, 1511, 1562
BsrI ACTGG 2 cut(s) 398, 1692
BssECI CCNNGG 6 cut(s) 16, 63, 208, 637, 675, 684
BssMI GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
BssT1I CCWWGG 4 cut(s) 63, 208, 637, 684
Bst2UI CCWGG 3 cut(s) 261, 522, 1368
Bst4CI ACNGT 5 cut(s) 20, 518, 605, 1162, 1339
Bst6I CTCTTC 3 cut(s) 119, 495, 868
BstAPI GCANNNNNTGC 2 cut(s) 1127, 1402
BstBI TTCGAA 4 cut(s) 404, 577, 645, 1668
BstC8I GCNNGC 4 cut(s) 291, 810, 960, 1713
BstDEI CTNAG 5 cut(s) 192, 764, 1019, 1052, 1800
BstDSI CCRYGG 3 cut(s) 16, 208, 637
BstEII GGTNACC 1 cut(s) 921
BstF5I GGATG 1 cut(s) 342
BstFNI CGCG 1 cut(s) 1751
BstHHI GCGC 1 cut(s) 1270
BstKTI GATC 7 cut(s) 156, 751, 1105, 1327, 1549, 1687, 1755
BstMAI GTCTC 7 cut(s) 69, 416, 708, 812, 1618, 1623, 1700
BstMBI GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
BstMWI GCNNNNNNNGC 9 cut(s) 286, 619, 725, 900, 1127, 1274, 1402, 1522, 1721
BstNI CCWGG 3 cut(s) 261, 522, 1368
BstNSI RCATGY 2 cut(s) 293, 962
BstPI GGTNACC 1 cut(s) 921
BstSCI CCNGG 3 cut(s) 259, 520, 1366
BstSFI CTRYAG 2 cut(s) 1119, 1242
BstUI CGCG 1 cut(s) 1751
BstV1I GCAGC 8 cut(s) 105, 931, 1224, 1250, 1357, 1360, 1594, 1866
BstV2I GAAGAC 1 cut(s) 1468
BstX2I RGATCY 2 cut(s) 1102, 1546
BstXI CCANNNNNNTGG 1 cut(s) 215
BstYI RGATCY 2 cut(s) 1102, 1546
BsuI GTATCC 1 cut(s) 1242
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 3 cut(s) 16, 208, 637
BtsCI GGATG 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 413
BtsIMutI CAGTG 2 cut(s) 413, 1257
BtuMI TCGCGA 1 cut(s) 1751
Cac8I GCNNGC 4 cut(s) 291, 810, 960, 1713
CaiI CAGNNNCTG 2 cut(s) 516, 1127
CfoI GCGC 1 cut(s) 1270
Cfr13I GGNCC 2 cut(s) 233, 1190
Csp6I GTAC 3 cut(s) 375, 409, 664
CspCI CAANNNNNGTGG 2 cut(s) 1164, 1199
CviAII CATG 7 cut(s) 209, 281, 290, 638, 918, 959, 1552
CviQI GTAC 3 cut(s) 375, 409, 664
DdeI CTNAG 5 cut(s) 192, 764, 1019, 1052, 1800
DpnI GATC 7 cut(s) 155, 750, 1104, 1326, 1548, 1686, 1754
DpnII GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
DraI TTTAAA 1 cut(s) 883
DraIII CACNNNGTG 1 cut(s) 1055
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 3 cut(s) 119, 495, 868
EarI CTCTTC 3 cut(s) 119, 495, 868
Ecl136II GAGCTC 2 cut(s) 139, 808
Eco130I CCWWGG 4 cut(s) 63, 208, 637, 684
Eco24I GRGCYC 2 cut(s) 141, 810
Eco47I GGWCC 2 cut(s) 233, 1190
Eco53kI GAGCTC 2 cut(s) 139, 808
Eco57I CTGAAG 3 cut(s) 1002, 1281, 1403
Eco91I GGTNACC 1 cut(s) 921
EcoICRI GAGCTC 2 cut(s) 139, 808
EcoO65I GGTNACC 1 cut(s) 921
EcoRI GAATTC 2 cut(s) 1297, 1362
EcoRII CCWGG 3 cut(s) 259, 520, 1366
EcoT14I CCWWGG 4 cut(s) 63, 208, 637, 684
EcoT22I ATGCAT 2 cut(s) 295, 1633
EcoT38I GRGCYC 2 cut(s) 141, 810
ErhI CCWWGG 4 cut(s) 63, 208, 637, 684
Esp3I CGTCTC 1 cut(s) 1623
FaeI CATG 7 cut(s) 212, 284, 293, 641, 921, 962, 1555
FatI CATG 7 cut(s) 208, 280, 289, 637, 917, 958, 1551
FbaI TGATCA 1 cut(s) 1684
Fnu4HI GCNGC 8 cut(s) 94, 945, 1213, 1239, 1346, 1349, 1608, 1855
FokI GGATG 1 cut(s) 349
FriOI GRGCYC 2 cut(s) 141, 810
Fsp4HI GCNGC 8 cut(s) 94, 945, 1213, 1239, 1346, 1349, 1608, 1855
FspBI CTAG 4 cut(s) 615, 1061, 1763, 1891
GlaI GCGC 1 cut(s) 1269
GluI GCNGC 8 cut(s) 94, 945, 1213, 1239, 1346, 1349, 1608, 1855
GsaI CCCAGC 1 cut(s) 932
GsuI CTGGAG 2 cut(s) 1454, 1742
HaeIII GGCC 1 cut(s) 207
HapII CCGG 2 cut(s) 419, 1328
HhaI GCGC 1 cut(s) 1270
Hin1II CATG 7 cut(s) 212, 284, 293, 641, 921, 962, 1555
Hin6I GCGC 1 cut(s) 1268
HinP1I GCGC 1 cut(s) 1268
HindIII AAGCTT 2 cut(s) 194, 974
HinfI GANTC 6 cut(s) 680, 820, 1115, 1124, 1318, 1373
HpaII CCGG 2 cut(s) 419, 1328
HphI GGTGA 5 cut(s) 296, 915, 1006, 1382, 1481
Hpy166II GTNNAC 2 cut(s) 268, 482
Hpy188I TCNGA 8 cut(s) 58, 237, 361, 679, 767, 1285, 1383, 1793
Hpy188III TCNNGA 7 cut(s) 566, 752, 980, 1061, 1322, 1697, 1750
Hpy8I GTNNAC 2 cut(s) 268, 482
HpyAV CCTTC 2 cut(s) 1393, 1555
HpyCH4III ACNGT 5 cut(s) 20, 518, 605, 1162, 1339
HpyCH4IV ACGT 1 cut(s) 582
HpyF10VI GCNNNNNNNGC 9 cut(s) 286, 619, 725, 900, 1127, 1274, 1402, 1522, 1721
HpyF3I CTNAG 5 cut(s) 192, 764, 1019, 1052, 1800
HpySE526I ACGT 1 cut(s) 582
Hsp92II CATG 7 cut(s) 212, 284, 293, 641, 921, 962, 1555
HspAI GCGC 1 cut(s) 1268
Ksp22I TGATCA 1 cut(s) 1684
Kzo9I GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
LguI GCTCTTC 2 cut(s) 119, 495
LmnI GCTCC 3 cut(s) 725, 1031, 1522
Lsp1109I GCAGC 8 cut(s) 105, 931, 1224, 1250, 1357, 1360, 1594, 1866
LweI GCATC 5 cut(s) 327, 820, 1276, 1383, 1805
MaeI CTAG 4 cut(s) 615, 1061, 1763, 1891
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 9 cut(s) 239, 386, 583, 626, 731, 921, 1303, 1407, 1586
MalI GATC 7 cut(s) 155, 750, 1104, 1326, 1548, 1686, 1754
MboI GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
MfeI CAATTG 1 cut(s) 1622
MflI RGATCY 2 cut(s) 1102, 1546
MhlI GDGCHC 3 cut(s) 141, 810, 858
MlsI TGGCCA 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 2 cut(s) 814, 1124
MmeI TCCRAC 4 cut(s) 932, 990, 1217, 1500
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 2 cut(s) 295, 1633
MroXI GAANNNNTTC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 207
MseI TTAA 6 cut(s) 882, 899, 972, 1077, 1502, 1542
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 928, 1241
MspI CCGG 2 cut(s) 419, 1328
MspR9I CCNGG 3 cut(s) 261, 522, 1368
MunI CAATTG 1 cut(s) 1622
Mva1269I GAATGC 2 cut(s) 1098, 1633
MvaI CCWGG 3 cut(s) 261, 522, 1368
MvnI CGCG 1 cut(s) 1751
MwoI GCNNNNNNNGC 9 cut(s) 286, 619, 725, 900, 1127, 1274, 1402, 1522, 1721
NcoI CCATGG 2 cut(s) 208, 637
NdeII GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
NlaIII CATG 7 cut(s) 212, 284, 293, 641, 921, 962, 1555
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 4 cut(s) 386, 921, 1407, 1586
NruI TCGCGA 1 cut(s) 1751
NsiI ATGCAT 2 cut(s) 295, 1633
NspI RCATGY 2 cut(s) 293, 962
NspV TTCGAA 4 cut(s) 404, 577, 645, 1668
PaeI GCATGC 2 cut(s) 293, 962
PciSI GCTCTTC 2 cut(s) 119, 495
PctI GAATGC 2 cut(s) 1098, 1633
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 4 cut(s) 680, 1124, 1318, 1373
PflMI CCANNNNNTGG 1 cut(s) 1487
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 8 cut(s) 95, 946, 1214, 1240, 1347, 1350, 1609, 1856
PleI GAGTC 2 cut(s) 814, 1123
PpsI GAGTC 2 cut(s) 814, 1123
PshBI ATTAAT 1 cut(s) 1077
PsiI TTATAA 2 cut(s) 351, 800
Psp124BI GAGCTC 2 cut(s) 141, 810
Psp6I CCWGG 3 cut(s) 259, 520, 1366
PspEI GGTNACC 1 cut(s) 921
PspFI CCCAGC 1 cut(s) 928
PspGI CCWGG 3 cut(s) 259, 520, 1366
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 233, 1190
PstI CTGCAG 1 cut(s) 1123
PstNI CAGNNNCTG 2 cut(s) 516, 1127
PsuI RGATCY 2 cut(s) 1102, 1546
PvuII CAGCTG 1 cut(s) 1241
RruI TCGCGA 1 cut(s) 1751
RsaI GTAC 3 cut(s) 376, 410, 665
RsaNI GTAC 3 cut(s) 375, 409, 664
RseI CAYNNNNRTG 1 cut(s) 213
SacI GAGCTC 2 cut(s) 141, 810
SapI GCTCTTC 2 cut(s) 119, 495
SaqAI TTAA 6 cut(s) 882, 899, 972, 1077, 1502, 1542
SatI GCNGC 8 cut(s) 94, 945, 1213, 1239, 1346, 1349, 1608, 1855
Sau3AI GATC 7 cut(s) 153, 748, 1102, 1324, 1546, 1684, 1752
Sau96I GGNCC 2 cut(s) 233, 1190
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 2 cut(s) 814, 1124
ScrFI CCNGG 3 cut(s) 261, 522, 1368
SduI GDGCHC 3 cut(s) 141, 810, 858
SfaNI GCATC 5 cut(s) 327, 820, 1276, 1383, 1805
SfcI CTRYAG 2 cut(s) 1119, 1242
SfuI TTCGAA 4 cut(s) 404, 577, 645, 1668
SinI GGWCC 2 cut(s) 233, 1190
SmiI ATTTAAAT 1 cut(s) 883
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 1 cut(s) 1412
SmoI CTYRAG 1 cut(s) 1412
SphI GCATGC 2 cut(s) 293, 962
SsiI CCGC 2 cut(s) 926, 1405
SspI AATATT 2 cut(s) 226, 1869
SspMI CTAG 4 cut(s) 615, 1061, 1763, 1891
SstI GAGCTC 2 cut(s) 141, 810
StyD4I CCNGG 3 cut(s) 259, 520, 1366
StyI CCWWGG 4 cut(s) 63, 208, 637, 684
SwaI ATTTAAAT 1 cut(s) 883
TaaI ACNGT 5 cut(s) 20, 518, 605, 1162, 1339
TaiI ACGT 1 cut(s) 585
TaqI TCGA 6 cut(s) 404, 577, 645, 1086, 1668, 1698
TatI WGTACW 2 cut(s) 408, 663
TfiI GAWTC 4 cut(s) 680, 1124, 1318, 1373
Tru1I TTAA 6 cut(s) 882, 899, 972, 1077, 1502, 1542
Tru9I TTAA 6 cut(s) 882, 899, 972, 1077, 1502, 1542
TscAI CASTG 2 cut(s) 420, 1264
TseFI GTSAC 4 cut(s) 386, 921, 1407, 1586
TseI GCWGC 8 cut(s) 93, 944, 1212, 1238, 1345, 1348, 1607, 1854
Tsp45I GTSAC 4 cut(s) 386, 921, 1407, 1586
TspDTI ATGAA 4 cut(s) 17, 288, 585, 1861
TspGWI ACGGA 4 cut(s) 674, 769, 1044, 1417
TspRI CASTG 2 cut(s) 420, 1264
Van91I CCANNNNNTGG 1 cut(s) 1487
VpaK11BI GGWCC 2 cut(s) 233, 1190
VspI ATTAAT 1 cut(s) 1077
XapI RAATTY 6 cut(s) 572, 847, 878, 1297, 1362, 1776
XbaI TCTAGA 1 cut(s) 1060
XceI RCATGY 2 cut(s) 293, 962
XcmI CCANNNNNNNNNTGG 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 4 cut(s) 615, 1061, 1763, 1891
ZrmI AGTACT 1 cut(s) 410
Zsp2I ATGCAT 2 cut(s) 295, 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.